CN109260502A - A kind of fibroin hyaluronic acid mandruka and its preparation method and application - Google Patents
A kind of fibroin hyaluronic acid mandruka and its preparation method and application Download PDFInfo
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- CN109260502A CN109260502A CN201811495485.0A CN201811495485A CN109260502A CN 109260502 A CN109260502 A CN 109260502A CN 201811495485 A CN201811495485 A CN 201811495485A CN 109260502 A CN109260502 A CN 109260502A
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- fibroin
- hyaluronic acid
- mandruka
- silk
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/225—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
Abstract
The invention discloses a kind of fibroin albumen hyaluronic acid mandrukas and preparation method and application without using chemical reagent processing.The method comprises the steps of firstly, preparing silk fibroin water solutions;Then silk fibroin protein solution and hyaluronic acid solution are uniformly mixed, it is injected into metal die, it is freezed under conditions of -10~-80 DEG C, 48~96h is handled under the conditions of Frozen Body is placed in 0~7 DEG C again, freeze-drying process is finally carried out, the fibroin albumen hyaluronic acid mandruka for being insoluble in water is formed.The material has good water suction performance of keeping humidity, can provide moist environment for the surface of a wound;Simultaneously, preparation process mild condition of the present invention, insoluble processing is realized by the physical crosslinking of fibroin albumen and hyaluronic acid, it does not need to add any chemical cross-linking agent and denaturant, it is easy to be controllable, it is easy to industrialization, and can effectively keeps the biocompatibility of material and the growth factor of efficient loading bioactivity.
Description
Technical field
The invention belongs to technical field of biological material, and in particular to a kind of fibroin hyaluronic acid mandruka and preparation method thereof and
Using fibroin hyaluronic acid mandruka produced by the present invention can be used for Wound dressing.
Background technique
Covering of the medical dressing as wound can substitute impaired skin and play temporarily in wound healing process
The barrier action of when property avoids or controls wound infection, provides suitable microenvironment for wound healing.In recent years, fibroin albumen, thoroughly
The natural high polymers materials such as bright matter acid are received significant attention as the application of functional dressing.Silk fibroin has excellent
Biocompatibility, mechanical performance and degradability, have a extensive future as bio-medical material.The moisturizing however, fibroin albumen absorbs water
Performance is insufficient, it is difficult to the microenvironment soaked for a long time is provided for the surface of a wound.Hyaluronic acid is a kind of natural mucopolysaccharide, in human skin
In be widely present, have excellent moisture-keeping function, be ideal natural moisturizing factor.Therefore, in conjunction with two kinds of natural high polymers
Feature can prepare the mandruka material with good structural stability and performance of keeping humidity, be used for Wound dressing.But
Since fibroin and hyaluronic acid belong to water soluble polymer, need when preparing intermingling material using chemical cross-linking agent.If
Can find it is a kind of mandruka material not soluble in water is prepared without using chemical cross-linking agent, for fibroin hyaluronic acid in life
Application and industrialization in object medical dressing will have great importance.
The fibroin albumen of freezing saves 2 by the patent report of Publication No. CN101502669B at -5~-25 DEG C~
60d obtains silk fibroin porous material not soluble in water.The patent report of Publication No. CN105749343A is by the silk of freezing
Fibroin saves 2~168h at 0~-7 DEG C and porous material insoluble under the acquisition hygrometric state that thaws at 37 DEG C.The preparation of these methods
Fibroin porous material is not needed using chemical cross-linking agent and denaturant, but single fibroin material water suction and moisture retention is poor.
Patent publication No. is a kind of fibroin albumen hyaluronic acid prepared by chemical cross-linking agent of the patent report of CN102836465A
Gel rubber material, by being crosslinked, hyaluronic acid significantly improves the water absorbing properties of fibroin material, but this method needs chemistry
The use of crosslinking agent.A kind of patent report of the Publication No. CN104497327A fibroin hyaluronic acid composite membrane of cross-linking agent-free
Preparation method, the blend film of water is insoluble in using drying and forming-film slow at 37 DEG C preparation, but film is as Wound dressing application
It is limited.There is research also to prepare fibroin albumen hyaluronic acid plural gel, but gel mechanical property using the method for ultrasonic wave induction
Difference is not able to satisfy the application of dressing, and ultrasonic wave acts on can degrade fibroin albumen and hyaluronic acid macromolecular weight.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of fibroin hyaluronic acid mandruka and preparation method thereof and
Using.Fibroin hyaluronic acid mandruka produced by the present invention has good water imbibition, and preparation method of the present invention is not used
Any chemical cross-linking agent, it is environment-protecting and non-poisonous;In addition, fibroin hyaluronic acid mandruka produced by the present invention can be used as Wound dressing.
In order to realize above-mentioned first purpose of the present invention, the present invention adopts the following technical scheme:
A kind of fibroin hyaluronic acid mandruka, the fibroin hyaluronic acid mandruka are insoluble in water, have aperture
For 10~500 μm of the hole being mutually communicated;The fibroin hyaluronic acid mandruka includes fibroin albumen and hyaluronic acid,
It is in terms of 100% by the gross mass of mandruka, in which: the mass fraction of hyaluronic acid is 0.5~10%.
Further, above-mentioned technical proposal, the fibroin albumen primary structure are I structure of silk, are insoluble in water.
Second object of the present invention is to provide the preparation method of fibroin hyaluronic acid mandruka described above, institute
The method of stating includes the following steps:
(1) silk fibroin protein solution that mass percentage concentration is 2~10% is prepared, is then added into the silk fibroin protein solution
Enter hyaluronic acid or hyaluronate solution, is sufficiently mixed uniformly, obtains fibroin albumen-hyaluronic acid mixed solution;
(2) mixed solution that step (1) obtains is injected into metal die, under the conditions of -10~-80 DEG C freeze 2~
4h obtains a Frozen Body;
(3) 48~96h is handled under the conditions of a Frozen Body for obtaining step (2) is placed in 0~-7 DEG C, obtains secondary freezing
Body;
(4) the secondary Frozen Body that step (3) obtains is freeze-dried, obtains the silk of the present invention for being insoluble in water
Plain hyaluronic acid mandruka.
Further, above-mentioned technical proposal, hyaluronate described in step (1) are hyaluroni, potassium hyaluronate
Any one of salt, hyaluronic acid magnesium salts, hyaluronic acid calcium salt, hyaluronic acid zinc salt or two or more combination.
Further, above-mentioned technical proposal, silk fibroin protein solution described in step (1) are that domestic silkworm silk is carried out degumming, molten
The regenerated silk fibroin solution obtained after solution, dialysis treatment.
Preferably, regenerated silk fibroin solution described above is made with the following method, includes the following steps:
(a) natural silk is carried out boiling processing in the sodium carbonate liquor that mass fraction is 0.05~1.0%, makes silk
Degumming obtains pure silk cellulose fiber after the silk of degumming is sufficiently then washed drying;
(b) the pure silk cellulose fiber that step (a) obtains is added in lysate, is then stirred under the conditions of 60~80 DEG C molten
1~2h is solved, fibroin albumen mixed solution is obtained;
(c) the fibroin albumen mixed solution that step (b) obtains is fitted into bag filter, with 2~4d of deionized water dialysis, is obtained
To silk fibroin water solution.
Preferably, lysate described in above-mentioned technical proposal step (b) is LiBr solution or calcium nitrate solution;Described
Lysate can also be the ternary mixed solution being made of calcium chloride, saturation unitary fatty alcohol, deionized water.
It is highly preferred that saturation unitary fatty alcohol described in above-mentioned technical proposal is appointing in methanol, ethyl alcohol, propyl alcohol and butanol
It is one or more kinds of.
Compared with prior art, the invention has the following beneficial effects:
(1) the fibroin albumen hyaluronic acid mandruka of water is insoluble in using the method formation of freezing due to the present invention, is kept away
The biocompatibility of material is effectively guaranteed in the use for having exempted from chemical reagent.
(2) fibroin albumen hyaluronic acid mandruka made from the method for the present invention has good water suction performance of keeping humidity, energy
Enough moist environment is provided for the surface of a wound;
(3) preparation process mild condition of the present invention is prepared for whole water phase, without using any chemical cross-linking agent and denaturation
Agent also avoids the conditions such as high temperature, high pressure, so as to realize the growth factor of efficient loading bioactivity;
(4) the method for the present invention is easy to be controllable, is easy to industrialization.
Specific embodiment
Below by specific implementation case, invention is further described in detail.The implementation case is with the technology of the present invention
Premised under implemented, now provide detailed embodiment and specific operating process illustrate the present invention it is creative,
But protection scope of the present invention case study on implementation not limited to the following.
The information for including according to the application, to those skilled in the art can be easily to essence of the invention
Really description carries out various changes, without departing from spirit and scope of the appended claims.It should be understood that the scope of the present invention is not
Process, property defined by being confined to or component, because these embodiments and other descriptions are just for the sake of schematic
Illustrate certain aspects of the present disclosure.In fact, this field or those skilled in the relevant art obviously can be to embodiment party of the present invention
The various changes that formula is made all cover within the scope of the appended claims.
It is not intended to limit the scope of the invention for a better understanding of the present invention, expression dosage used in this application,
All numbers of percentage and other numerical value, are understood to be modified with word " about " in all cases.Therefore,
Unless stated otherwise, otherwise digital parameters listed in specification and appended book are all approximations, may
It can be changed according to the difference for the desirable properties for attempting to obtain.Each digital parameters at least should be considered as according to being reported
Effective digital and obtained by the conventional method of rounding up.
Embodiment 1
A kind of preparation method of fibroin hyaluronic acid mandruka of the present embodiment, described method includes following steps:
(1) preparation of regenerated silk fibroin solution
(a) natural silk is boiled into place in the sodium carbonate liquor that mass fraction is 0.05% under the conditions of 98~100 DEG C
30min is managed, is repeated 3 times, makes natural silk degumming, obtains pure silk cellulose fiber after the silk of degumming is sufficiently then washed drying;
(b) the pure silk cellulose fiber that step (a) obtains is added in the LiBr solution that concentration is 9.3mol/L, then 60
Stirring and dissolving 2h under the conditions of DEG C obtains fibroin albumen mixed solution;
(c) the fibroin albumen mixed solution that step (b) obtains is fitted into bag filter, with deionized water dialysis 3d, is obtained
Silk fibroin water solution;
(2) preparation of fibroin hyaluronic acid mandruka
(i) mass percentage concentration for the silk fibroin water solution that above-mentioned steps (c) obtain is adjusted to 2%, then to institute
It states and sodium hyaluronate solution is added in silk fibroin water solution, be sufficiently mixed uniformly, it is molten to obtain fibroin albumen-hyaluronic acid mixing
Liquid, the content of Sodium Hyaluronate is 0.5wt% in mixed solution;
(ii) mixed solution for obtaining step (i) is injected into metal die, is freezed 2h under the conditions of -80 DEG C, is obtained
Frozen Body;
(iii) Frozen Body for obtaining step (ii) handles 96h under the conditions of being placed in -7 DEG C, obtains secondary Frozen Body;
(iiii) the secondary Frozen Body for obtaining step (iii) obtains difficulty of the present invention in -80 DEG C of freeze-drying 2h
It is dissolved in the fibroin hyaluronic acid mandruka of water, it is 10~500 μm of the hole being mutually communicated that the mandruka, which has aperture,
Gap.
The present embodiment fibroin hyaluronic acid mandruka obtained above is placed in deionized water, is impregnated under the conditions of 37 DEG C
For 24 hours, weighing calculates it is found that sponge dissolve-loss ratio is only 2.8%.
Embodiment 2
A kind of preparation method of fibroin hyaluronic acid mandruka of the present embodiment, described method includes following steps:
(1) preparation of regenerated silk fibroin solution
(a) natural silk is boiled into place in the sodium carbonate liquor that mass fraction is 0.05% under the conditions of 98~100 DEG C
30min is managed, is repeated 3 times, makes natural silk degumming, obtains pure silk cellulose fiber after the silk of degumming is sufficiently then washed drying;
(b) the pure silk cellulose fiber that step (a) obtains is added to the CaCl that molar ratio is 1:2:82/CH3CH2OH/H2O tri-
In first solution, then stirring and dissolving 1h under the conditions of 70 DEG C, obtains fibroin albumen mixed solution;
(c) the fibroin albumen mixed solution that step (b) obtains is fitted into bag filter, with deionized water dialysis 3d, is obtained
Silk fibroin water solution;
(2) preparation of fibroin hyaluronic acid mandruka
(i) mass percentage concentration for the silk fibroin water solution that above-mentioned steps (c) obtain is adjusted to 5%, then to institute
Addition hyaluronic acid potassium solution in silk fibroin water solution is stated, is sufficiently mixed uniformly, it is molten to obtain fibroin albumen-hyaluronic acid mixing
Liquid, the content of Sodium Hyaluronate is 2.5wt% in mixed solution;
(ii) mixed solution for obtaining step (i) is injected into metal die, is freezed 3h under the conditions of -60 DEG C, is obtained
Frozen Body;
(iii) Frozen Body for obtaining step (ii) handles 72h under the conditions of being placed in -4 DEG C, obtains secondary Frozen Body;
(iiii) the secondary Frozen Body for obtaining step (iii) obtains difficulty of the present invention in -80 DEG C of freeze-drying 2h
It is dissolved in the fibroin hyaluronic acid mandruka of water, it is 10~500 μm of the hole being mutually communicated that the mandruka, which has aperture,
Gap.
The present embodiment fibroin hyaluronic acid mandruka obtained above is placed in deionized water, is impregnated under the conditions of 37 DEG C
For 24 hours, weighing calculates it is found that sponge dissolve-loss ratio is only 3.2%.
Embodiment 3
A kind of preparation method of fibroin hyaluronic acid mandruka of the present embodiment, described method includes following steps:
(1) preparation of regenerated silk fibroin solution
(a) natural silk is boiled into place in the sodium carbonate liquor that mass fraction is 0.05% under the conditions of 98~100 DEG C
30min is managed, is repeated 3 times, makes natural silk degumming, obtains pure silk cellulose fiber after the silk of degumming is sufficiently then washed drying;
(b) the pure silk cellulose fiber that step (a) obtains is added in the calcium nitrate solution that concentration is 7.5mol/L, is then existed
Stirring and dissolving 2h under the conditions of 80 DEG C, obtains fibroin albumen mixed solution;
(c) the fibroin albumen mixed solution that step (b) obtains is fitted into bag filter, with deionized water dialysis 3d, is obtained
Silk fibroin water solution;
(2) preparation of fibroin hyaluronic acid mandruka
(i) mass percentage concentration for the silk fibroin water solution that above-mentioned steps (c) obtain is adjusted to 7.5%, then to
Sodium hyaluronate solution is added in the silk fibroin water solution, is sufficiently mixed uniformly, obtains fibroin albumen-hyaluronic acid mixing
Solution, the content of Sodium Hyaluronate is 7.5wt% in mixed solution;
(ii) mixed solution for obtaining step (i) is injected into metal die, is freezed 4h under the conditions of -40 DEG C, is obtained
Frozen Body;
(iii) Frozen Body for obtaining step (ii) handles 48h under the conditions of being placed in -1 DEG C, obtains secondary Frozen Body;
(iiii) the secondary Frozen Body for obtaining step (iii) obtains difficulty of the present invention in -80 DEG C of freeze-drying 2h
It is dissolved in the fibroin hyaluronic acid mandruka of water, it is 10~500 μm of the hole being mutually communicated that the mandruka, which has aperture,
Gap.
The present embodiment fibroin hyaluronic acid mandruka obtained above is placed in deionized water, is impregnated under the conditions of 37 DEG C
For 24 hours, weighing calculates it is found that sponge dissolve-loss ratio is only 3.0%.
Embodiment 4
A kind of preparation method of fibroin hyaluronic acid mandruka of the present embodiment, described method includes following steps:
(1) preparation of regenerated silk fibroin solution
(a) natural silk is boiled into place in the sodium carbonate liquor that mass fraction is 0.05% under the conditions of 98~100 DEG C
30min is managed, is repeated 3 times, makes natural silk degumming, obtains pure silk cellulose fiber after the silk of degumming is sufficiently then washed drying;
(b) the pure silk cellulose fiber that step (a) obtains is added in the calcium nitrate solution that concentration is 7.5mol/L, is then existed
Stirring and dissolving 1h under the conditions of 60 DEG C, obtains fibroin albumen mixed solution;
(c) the fibroin albumen mixed solution that step (b) obtains is fitted into bag filter, with deionized water dialysis 3d, is obtained
Silk fibroin water solution;
(2) preparation of fibroin hyaluronic acid mandruka
(i) mass percentage concentration for the silk fibroin water solution that above-mentioned steps (c) obtain is adjusted to 10%, then to institute
It states and sodium hyaluronate solution is added in silk fibroin water solution, be sufficiently mixed uniformly, it is molten to obtain fibroin albumen-hyaluronic acid mixing
Liquid, the content of Sodium Hyaluronate is 10wt% in mixed solution;
(ii) mixed solution for obtaining step (i) is injected into metal die, is freezed 2h under the conditions of -40 DEG C, is obtained
Frozen Body;
(iii) Frozen Body for obtaining step (ii) handles 72h under the conditions of being placed in -4 DEG C, obtains secondary Frozen Body;
(iiii) the secondary Frozen Body for obtaining step (iii) obtains difficulty of the present invention in -80 DEG C of freeze-drying 2h
It is dissolved in the fibroin hyaluronic acid mandruka of water, it is 10~500 μm of the hole being mutually communicated that the mandruka, which has aperture,
Gap.
The present embodiment fibroin hyaluronic acid mandruka obtained above is placed in deionized water, is impregnated under the conditions of 37 DEG C
For 24 hours, weighing calculates it is found that sponge dissolve-loss ratio is only 3.0%.
Embodiment 5
A kind of preparation method of fibroin hyaluronic acid mandruka of the present embodiment, described method includes following steps:
(1) preparation of regenerated silk fibroin solution
(a) natural silk is boiled into place in the sodium carbonate liquor that mass fraction is 0.5% under the conditions of 98~100 DEG C
30min is managed, is repeated 3 times, makes natural silk degumming, obtains pure silk cellulose fiber after the silk of degumming is sufficiently then washed drying;
(b) the pure silk cellulose fiber that step (a) obtains is added in the LiBr solution that concentration is 9.3mol/L, then 65
Stirring and dissolving 1.5h under the conditions of DEG C obtains fibroin albumen mixed solution;
(c) the fibroin albumen mixed solution that step (b) obtains is fitted into bag filter, with deionized water dialysis 4d, is obtained
Silk fibroin water solution;
(2) preparation of fibroin hyaluronic acid mandruka
(i) mass percentage concentration for the silk fibroin water solution that above-mentioned steps (c) obtain is adjusted to 2%, then to institute
It states and sodium hyaluronate solution is added in silk fibroin water solution, be sufficiently mixed uniformly, it is molten to obtain fibroin albumen-hyaluronic acid mixing
Liquid, the content of Sodium Hyaluronate is 0.5wt% in mixed solution;
(ii) mixed solution for obtaining step (i) is injected into metal die, is freezed 4h under the conditions of -10 DEG C, is obtained
Frozen Body;
(iii) Frozen Body for obtaining step (ii), which is placed under the conditions of 0 DEG C, handles 96h, obtains secondary Frozen Body;
(iiii) the secondary Frozen Body for obtaining step (iii) obtains difficulty of the present invention in -80 DEG C of freeze-drying 2h
It is dissolved in the fibroin hyaluronic acid mandruka of water, it is 10~500 μm of the hole being mutually communicated that the mandruka, which has aperture,
Gap.
The present embodiment fibroin hyaluronic acid mandruka obtained above is placed in deionized water, is impregnated under the conditions of 37 DEG C
For 24 hours, weighing calculates it is found that sponge dissolve-loss ratio is only 2.9%.
Claims (8)
1. a kind of fibroin hyaluronic acid mandruka, it is characterised in that: the fibroin hyaluronic acid mandruka is insoluble in water,
The hole being mutually communicated for being 10~500 μm with aperture;The fibroin hyaluronic acid mandruka include fibroin albumen and
Hyaluronic acid is in terms of 100% by the gross mass of mandruka, in which: the mass fraction of hyaluronic acid is 0.5~10%.
2. fibroin hyaluronic acid mandruka according to claim 1, it is characterised in that: the fibroin albumen primary structure
For I structure of silk, it is insoluble in water.
3. the preparation method of fibroin hyaluronic acid mandruka described in claim 1, it is characterised in that: the method includes such as
Lower step:
(1) silk fibroin protein solution that mass percentage concentration is 2~10% is prepared, is then added into the silk fibroin protein solution saturating
Bright matter acid or hyaluronate solution, are sufficiently mixed uniformly, obtain fibroin albumen-hyaluronic acid mixed solution;
(2) mixed solution that step (1) obtains is injected into metal die, 2~4h is freezed under the conditions of -10~-80 DEG C, is obtained
To a Frozen Body;
(3) 48~96h is handled under the conditions of a Frozen Body for obtaining step (2) is placed in 0~-7 DEG C, obtains secondary Frozen Body;
(4) the secondary Frozen Body that step (3) obtains is freeze-dried, it is saturating obtains the fibroin of the present invention for being insoluble in water
Bright matter acid mandruka.
4. the preparation method of fibroin hyaluronic acid mandruka according to claim 3, it is characterised in that: in step (1)
The hyaluronate is hyaluroni, Hyaluronic acid potassium salt, hyaluronic acid magnesium salts, hyaluronic acid calcium salt, hyaluronic acid
Any one of zinc salt or two or more combination.
5. the preparation method of fibroin hyaluronic acid mandruka according to claim 3, it is characterised in that: in step (1)
The silk fibroin protein solution is the regenerated silk fibroin solution for obtain after degumming, dissolution, dialysis treatment by domestic silkworm silk.
6. the preparation method of fibroin hyaluronic acid mandruka according to claim 5, it is characterised in that: the regeneration
Silk fibroin protein solution is made with the following method, includes the following steps:
(a) natural silk is carried out boiling processing in the sodium carbonate liquor that mass fraction is 0.05~1.0%, keeps silk de-
Glue obtains pure silk cellulose fiber after the silk of degumming is sufficiently then washed drying;
(b) the pure silk cellulose fiber that step (a) obtains is added in lysate, then the stirring and dissolving 1 under the conditions of 60~80 DEG C
~2h obtains fibroin albumen mixed solution;
(c) the fibroin albumen mixed solution that step (b) obtains is fitted into bag filter, with 2~4d of deionized water dialysis, obtains silk
Fibroin aqueous solution.
7. the preparation method of fibroin hyaluronic acid mandruka according to claim 6, it is characterised in that: in step (b)
The lysate be LiBr solution or calcium nitrate solution or be made of calcium chloride, saturation unitary fatty alcohol, deionized water three
First mixed solution.
8. the preparation method of fibroin hyaluronic acid mandruka according to claim 7, it is characterised in that: the saturation
Unitary fatty alcohol is any one of methanol, ethyl alcohol, propyl alcohol and butanol or a variety of.
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Cited By (3)
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CN112023108A (en) * | 2020-09-02 | 2020-12-04 | 中山大学附属第一医院 | Three-dimensional dressing and preparation method thereof |
CN112245647A (en) * | 2020-10-26 | 2021-01-22 | 杭州电子科技大学 | Preparation method of sponge wound dressing |
CN115252889A (en) * | 2022-07-15 | 2022-11-01 | 武汉纺织大学 | Oxygen-generating stent and preparation method thereof |
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