CN109258711B - Application of abrus oil ether extract in preventing and treating vegetable pests - Google Patents
Application of abrus oil ether extract in preventing and treating vegetable pests Download PDFInfo
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- CN109258711B CN109258711B CN201811290152.4A CN201811290152A CN109258711B CN 109258711 B CN109258711 B CN 109258711B CN 201811290152 A CN201811290152 A CN 201811290152A CN 109258711 B CN109258711 B CN 109258711B
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- 239000012259 ether extract Substances 0.000 title claims abstract description 38
- 235000013311 vegetables Nutrition 0.000 title claims abstract description 30
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 27
- 241000220436 Abrus Species 0.000 title claims abstract description 9
- 239000003208 petroleum Substances 0.000 claims abstract description 37
- 108010066676 Abrin Proteins 0.000 claims abstract description 16
- 241000500441 Plutellidae Species 0.000 claims abstract description 14
- 239000005871 repellent Substances 0.000 claims description 10
- 230000002940 repellent Effects 0.000 claims description 10
- 241000220479 Acacia Species 0.000 claims description 6
- 235000010643 Leucaena leucocephala Nutrition 0.000 claims description 6
- 239000002917 insecticide Substances 0.000 claims description 2
- 241000982105 Brevicoryne brassicae Species 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 10
- 239000000575 pesticide Substances 0.000 abstract description 7
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 206010059866 Drug resistance Diseases 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 241000238631 Hexapoda Species 0.000 description 18
- 238000011282 treatment Methods 0.000 description 13
- 238000012360 testing method Methods 0.000 description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 10
- 241001046947 Ectropis obliqua Species 0.000 description 8
- 239000003814 drug Substances 0.000 description 8
- 210000000582 semen Anatomy 0.000 description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 230000001846 repelling effect Effects 0.000 description 6
- 241000500437 Plutella xylostella Species 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000002265 prevention Effects 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000002147 killing effect Effects 0.000 description 4
- 238000002386 leaching Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000005868 ontogenesis Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 241001124076 Aphididae Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- OGFXBIXJCWAUCH-KPHUOKFYSA-N (+)-isolariciresinol Chemical compound C1([C@@H]2[C@@H](CO)[C@H](CO)CC=3C=C(C(=CC=32)O)OC)=CC=C(O)C(OC)=C1 OGFXBIXJCWAUCH-KPHUOKFYSA-N 0.000 description 1
- 244000144619 Abrus precatorius Species 0.000 description 1
- 241000273311 Aphis spiraecola Species 0.000 description 1
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 1
- 241000510667 Conioselinum Species 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 241001093152 Mangifera Species 0.000 description 1
- 241000255969 Pieris brassicae Species 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000001941 cymbopogon citratus dc and cymbopogon flexuosus oil Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- OEZZQOVAJDAVSJ-UHFFFAOYSA-N isolariciresinol Natural products COc1cc(ccc1O)C1C(CO)C(CO)Cc2cc(O)c(OC)cc12 OEZZQOVAJDAVSJ-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000010663 parsley oil Substances 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 244000089052 wirilda Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Catching Or Destruction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an application of an abrin petroleum ether extract in controlling vegetable pests, belonging to the technical field of biological pesticides. The invention adopts the abrin petroleum ether extract for preventing and controlling vegetable pests for the first time, and experiments show that the abrin petroleum ether extract has obvious placing effect on cabbage aphids and diamond back moths and has long validity period; the abrus petroleum ether extract is derived from plants, is easy to degrade in nature, is safe to the environment, does not pollute grains, and is not easy to generate drug resistance by pests.
Description
Technical Field
The invention belongs to the technical field of biological pesticides, and particularly relates to an application of an abrin petroleum ether extract in controlling vegetable pests.
Background
Vegetables are indispensable non-staple food in people's daily life, and the vegetables industry plays an important position in national economy. Since the 20 th century and the 50 th century, in particular, the reform was opened, the Chinese vegetable industry realized rapid development, was the first crop in terms of output value, export quantity and the like, and became the post industry for the income increase of farmers and the development of rural areas in China. The harm of vegetable pests is a main factor causing the reduction of the yield and the quality of vegetables, and the unreasonable use of pesticides in the vegetable pest control process is the most direct cause of the quality safety problem of vegetable products. Therefore, the prevention and control of vegetable pests are related to the national civilization, social stability and physical health of people. At present, the most main method and means for controlling vegetable pests internationally are chemical control, but the problem of '3R' caused by the chemical control is increasingly serious, so that the development of a novel environment-friendly vegetable pesticide has important significance. Because of the problems faced by the current ecology and environment, researchers at home and abroad devote to the development of a novel green protection means. For the prevention and treatment of drugs, plant-based insecticidal substances are widely used.
For example, chinese patent 201710520509.2 discloses a botanical pesticide and its preparation method and use, the main effective component of the botanical pesticide is the isolariciresinol, the component exists in large amount in nature, especially the content in plant is high, the toxicity is low, it is easy to degrade, it is safe to environment; is suitable for killing diamondback moth or cabbage caterpillar. Chinese patent 201010185447.2 discloses a botanical synergistic compound aphicide and a preparation method thereof, wherein the preparation contains hemlock parsley oil and lemongrass oil, and the mixture is mainly applied to control Aphis citricola, Aphis brassicae and other aphids on fruit trees, vegetables and flowers after being mixed and compounded; the botanical synergistic compound aphicide does not contain any chemical pesticide component, has great application and popularization values in the production of organic agricultural products, and is an ideal novel pollution-free aphicide.
The semen abri petroleum ether extract is prepared by pulverizing semen abri (Abrus precatorius L.) of Leguminosae, and extracting with organic solvent petroleum ether. At present, however, the petroleum ether extract of the abrus seeds is rarely applied to the prevention and control of vegetable pests.
Disclosure of Invention
1. Problems to be solved
Aiming at the problem that the existing semen abri petroleum ether extract is rarely applied to the prevention and control of vegetable pests, the invention provides the application of the semen abri petroleum ether extract to the prevention and control of the vegetable pests, and the semen abri petroleum ether extract can effectively prevent and control cabbage aphids and plutella xylostella.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
Application of semen abri petroleum ether extract in preventing and treating vegetable pests is provided.
Preferably, the vegetable pests include cabbage aphids and diamondback moths.
Preferably, the application of the abrin petroleum ether extract as a repellent in controlling vegetable pests is provided.
Preferably, the concentration of the petroleum ether extract of abrin is 1mg/ml-25 mg/ml.
Preferably, the application of the abrin petroleum ether extract as a contact insecticide in controlling vegetable pests.
Preferably, the concentration of the petroleum ether extract of abrin is 3.125mg/ml-50 mg/ml.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
the invention adopts the abrin petroleum ether extract for preventing and controlling vegetable pests for the first time, and experiments show that the abrin petroleum ether extract has obvious placing effect on cabbage aphids and diamond back moths and has long validity period; the abrus petroleum ether extract is derived from plants, is easy to degrade in nature, is safe to the environment, does not pollute grains, and is not easy to generate drug resistance by pests.
Detailed Description
The invention is further described with reference to specific examples.
Example 1
Acacia petroleum ether extract as repellent for repelling cabbage aphid, diamondback moth and tea geometrid
1. Test insects
Cabbage aphids: provided by university of agriculture in Anhui. The lighting cycle of aphid growth is L: d ═ 16: 8, selecting healthy fourth-instar larvae with consistent ontogeny for testing, wherein the culture temperature is 25 +/-1 ℃.
And (3) diamondback moth: provided by insect rearing rooms of the university of agriculture in Anhui. Cabbage seedlings cultured by vermiculite in an insect rearing room (25 ℃, RH 70% -80%, L: D is 12: 12h) are raised, and healthy second-instar larvae with consistent ontogenesis are selected for testing.
Tea geometrid: provided by insect rearing rooms of the university of agriculture in Anhui. Fresh tender tea is fed in an insect feeding room (25 ℃, RH 70% -80%, L: D is 12: 12h), and healthy third instar larvae with consistent ontogenesis are selected for testing.
2. Petroleum ether extract of acacia for test:
the seeds of the abrus are purchased in the big market of traditional Chinese medicinal materials in Bozhou, the seeds are cleaned and dried in the shade, a grinder is used for grinding, and the ground seeds are soaked in petroleum ether; the treatment temperature is room temperature, and the treatment time is one week; performing vacuum filtration after one week, collecting filtrate obtained after the filtration, continuously leaching filter residue obtained after the filtration with petroleum ether, repeating leaching operation for three times, and collecting leaching solution obtained after leaching; and finally, transferring the filtrate and the leacheate into a rotary evaporator for rotary evaporation to obtain the abrin petroleum ether extract, and refrigerating the abrin petroleum ether extract in a refrigerator at 4 ℃ for later use.
3. Test method
Adopts a blade drug film method. Dividing cabbage leaves with the diameter of 9cm into two equal semicircular blades, putting the leaves into a culture dish with the same diameter, diluting the petroleum ether extract of the semen abri with acetone into liquid medicines with different concentrations, respectively transferring 1mL of the liquid medicine with each concentration by using a liquid transfer gun, uniformly coating the liquid medicine on the leaves of an experimental group, coating 1mL of acetone on the other control blade, after the acetone is completely volatilized, inoculating 30 insects to be tested into each dish, and covering a wire netting to prevent the insects to be tested from escaping. Each treatment was repeated 3 times. And (4) placing each treatment in an insect breeding incubator, checking the number of insects on the treated side and the control side after 24, 48 and 72 hours of treatment respectively, and calculating the repellency rate.
Grading standard of the repellent rate:
level 0: the repelling rate is 0; grade III: the repelling rate is 40.1-60%;
stage I: the repelling rate is 0.1-20%; stage IV: the repelling rate is 60.1-80%;
II stage: the repelling rate is 20.1-40%; and V stage: the repellent rate is 80.1-100%;
TABLE 1
As can be seen from Table 1: the acacia petroleum ether extract has obvious repellent effect on cabbage aphids and diamondback moths, and the higher the dosage, the greater the repellent effect; the test insects were treated at a dose of 25mg/mL, and the average repellency rates for cabbage aphids, diamond back moths and tea geometrids were 84.60%, 67.74% and 46.35%, respectively, with the repellency ratings being V, IV and III, respectively. But when the dosage is more than 25mg/ml, the economic cost is increased, and the effect is not obviously increased; the repellent effect of the abrin petroleum ether extract on the cabbage aphids is higher than that of the diamond back moths under the same dosage, and the repellent rate of the tea geometrid on tea tree pests is far less than that of vegetable pests (the cabbage aphids and the diamond back moths); in addition, at the same dosage, the concentration is reduced due to the volatilization of the liquid medicine along with the prolonging of the treatment time, so that the repellent effect is reduced in the whole.
Example 2
The abrus oil ether extract as contact killer has contact killing effect on cabbage aphid, diamond back moth and tea geometrid
1. Test insects
The tested insects, i.e. cabbage aphids, diamondback moths and tea geometrids, are the same as in example 1.
2. Petroleum ether extract of acacia longissima for testing
The petroleum ether extract of acacia mangifera to be tested was the same as in example 1.
3. Test method
The dropping method is adopted. Putting filter paper with the diameter of 9cm into a culture dish with the same diameter, diluting the petroleum ether extract into liquid medicines with different concentrations by using acetone, selecting 30 tested insects with basically consistent growth conditions, placing the tested insects on the moisturized leaves in the culture dish, and adopting a micro-syringe with the use range of 5 mu l by adopting a dropping method. The test insects were spotted on their forebreast back plates using a micro-syringe, and about 0.5. mu.l of the drug solution was spotted on each test insect. Acetone was used as a blank control and treatment was repeated 3 times. The treatments were placed in an insect incubator and the death of the test insects was investigated at 24, 48 and 72 hours of treatment.
TABLE 2
From Table 2, it is known that the petroleum ether extract of abrus seeds has significant contact poisoning effect on cabbage aphids and diamondback moths. After 24 hours of treatment, when the concentration is 12.50mg/ml, the death rate of cabbage aphids reaches 87.42%, when the concentration is 25mg/ml, the death rates of diamond back moths and tea geometrids respectively reach 91.08% and 44.91%, the contact killing effect is obviously enhanced along with the increase of the treatment dosage, but when the dosage is more than 12.50mg/ml (for treating cabbage aphids) or more than 25mg/ml (for treating diamond back moths), the economic cost is improved, and the effect is reduced; in addition, at the same treatment dosage, the effect of the abrin petroleum ether extract on vegetable pests (cabbage aphids and diamond back moths) is higher than that of the tea plant pest, namely, tea geometrid, and the contact killing effect of the abrin petroleum ether extract is gradually enhanced along with the prolonging of time.
While the invention has been described in further detail in connection with specific embodiments thereof, it will be understood that the invention is not limited thereto, and that various other modifications and substitutions may be made by those skilled in the art without departing from the spirit of the invention, which should be considered to be within the scope of the invention as defined by the appended claims.
Claims (2)
1. The application of the abrin petroleum ether extract as a repellent in preventing and controlling vegetable pests is characterized in that:
the vegetable pests are diamondback moths;
the concentration of the acacia petroleum ether extract is 3.125mg/ml-50 mg/ml.
2. The application of the abrus oil ether extract as a contact insecticide in preventing and controlling vegetable pests is characterized in that:
the vegetable pests are diamondback moths;
the concentration of the acacia petroleum ether extract is 3.125mg/ml-50 mg/ml.
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| CN201811290152.4A CN109258711B (en) | 2018-10-31 | 2018-10-31 | Application of abrus oil ether extract in preventing and treating vegetable pests |
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| CN201811290152.4A CN109258711B (en) | 2018-10-31 | 2018-10-31 | Application of abrus oil ether extract in preventing and treating vegetable pests |
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| CN109258711B true CN109258711B (en) | 2021-08-24 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101361494A (en) * | 2008-09-28 | 2009-02-11 | 宁波市北仑区白峰山防大可桃子研究所 | Green non-poisonous pesticides and preparation method thereof |
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| CN106719936A (en) * | 2016-11-30 | 2017-05-31 | 河池市金城江区科学技术情报研究所 | It is exclusively used in insecticide of mulberry tree plantation and preparation method thereof |
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2018
- 2018-10-31 CN CN201811290152.4A patent/CN109258711B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101361494A (en) * | 2008-09-28 | 2009-02-11 | 宁波市北仑区白峰山防大可桃子研究所 | Green non-poisonous pesticides and preparation method thereof |
| CN106614831A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Insecticide for stevia rebaudiana |
| CN106719936A (en) * | 2016-11-30 | 2017-05-31 | 河池市金城江区科学技术情报研究所 | It is exclusively used in insecticide of mulberry tree plantation and preparation method thereof |
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