CN109258683B

CN109258683B - Ointment with internal absorption and conduction functions for preventing and treating longhorn beetles and using method thereof

Info

Publication number: CN109258683B
Application number: CN201811186679.2A
Authority: CN
Inventors: 管凌君; 付方平; 陈小军; 沈殿晶; 孟志远; 范添乐; 石祖贵; 杨文�; 胡珊; 任莉
Original assignee: Nanjing Yingzhikang Agricultural Science & Technology Co ltd; Yangzhou Nuofeng Agricultural Technology Co ltd
Current assignee: Nanjing Yingzhikang Agricultural Science & Technology Co ltd; Yangzhou Nuofeng Agricultural Technology Co ltd
Priority date: 2018-10-12
Filing date: 2018-10-12
Publication date: 2021-04-20
Anticipated expiration: 2038-10-12
Also published as: CN109258683A

Abstract

The invention discloses an ointment with a systemic conduction effect for preventing and treating longicorn, which belongs to the field of pesticides and preparations thereof and is prepared by mixing trifluorfluoropyrimidine, quinalphos and an auxiliary agent, wherein the weight percentage of the trifluorfluoropyrimidine and the quinalphos in the total weight of the ointment is 0.1-20%, and the balance of the auxiliary agent. The auxiliary agent comprises a solvent, an adhesive and a filler, wherein the solvent, the adhesive and the filler account for 0.1-80.0%, 0.1-80.0% and 0.1-80.0% of the total weight of the ointment respectively. The invention also discloses a using method of the ointment, which comprises the following steps: the ointment is applied to the stripped bark, has good adhesive force and persistence, is simple and convenient to apply, improves the utilization rate of the medicament, reduces the environmental pollution, can enter plants through the absorption of the plant epidermis, has certain contact killing effect, can prevent pests from crawling up and down on the trunk, and thus can greatly reduce the damage to the plants.

Description

Ointment with internal absorption and conduction functions for preventing and treating longhorn beetles and using method thereof

Technical Field

The invention relates to an ointment with a systemic conduction function for preventing and treating longicorn and a using method thereof, belonging to the field of pesticides and preparations thereof.

Background

The longhorn beetles are important trunk-boring pests of poplar, willow, elm, maple and other tree species in China, and more than 100 longhorn beetles are reported in China. As the longhorn beetles have hidden lives, few varieties of natural enemies and complex life history, the poplar and the willow are one of the first choice tree species for afforestation, wind prevention and sand fixation, fast growth and high yield and other afforestation in China, so that the food resources of the longhorn beetles are sufficient, the longhorn beetles are seriously damaged in China, and the annual occurrence area is 60 ten thousand hm²Left and right. Over 10 years, longhorn beetles and longhorn beetles spread rapidly and are seriously damaged, so that the development of poplar and willow is greatly limited, and the healthy development of forestry is seriously influenced. The great loss of longhorn beetles to forestry in China is closely related to the characteristics of life habits and harmful factors of longhorn beetles, so that an effective eradication method for the control of longhorn beetles in production is lacked, and the method is mainly characterized in that: (1) the natural cattle has hidden life, less natural enemy types, large environment accommodation capacity, weak natural control acting force and strong individual survival adaptability. The existence of the artificial ecological bed is not easily threatened by severe natural geographical climate environment and the like. Once introduced into an area, it is difficult to completely destroy it. (2) The generation differentiation of the longicorn is complex, the generation is long, the life cycle is more than 1 year, and the life history of the longicorn is variable and complex due to the huge difference of the individual niche, so that great difficulty is caused to the prevention and treatment work of the longicorn. (3) Strong fecundity, high survival rate of the population and rapid and stable population proliferation. The egg laying amount of the longicorn in each female is between 30 and 70, about 90 percent of the longicorn can be normally hatched, wherein more than 100 longicorn can be hatched. The long-horned beetle eggs are mostly bred and matured in batches, have long service life, and can be mated and laid eggs for many times. To be provided withIn the case of anoplophora glabripennis, the population trend index can be as high as 2-4 or higher on a suitable host. (4) Sufficient food resources and various hosts. Relates to salicaceae, elmaceae, Moraceae, mechanismaceae and the like, can be used as a food resource of longicorn, and provides necessary conditions for the continuation of the population.

The control of the longicorn is an irresistible task because the longicorn larvae live in hidden places and great difficulty is brought to the control work. The control of the longicorn at present adopts a comprehensive control strategy, and the specific conditions are as follows: (1) forest culture measures: and 4, selecting excellent varieties with insect resistance, such as medium forest No. 46, medium forest No. 108 and the like for afforestation, strictly performing seedling quarantine and avoiding afforestation of seedlings with insects. The new forestation is far away from a pest source, or an isolation zone is planted to build a mixed forest; the method is characterized in that the anoplophora chinensis anomala tree species and the poplar are mixed and planted, block-shaped mixing is preferably adopted generally, and the protective forest belt can be mixed and planted at intervals in sections. Measures such as big holes, big seedlings, big water, frequent fertilization, frequent loosening and the like are adopted to promote the healthy and rapid growth of the forest and enhance the insect resistance (Pan hong Yang and the like, 2005). (2) Physical measures are as follows: the mulberry longicorn is active at night, has strong phototaxis and can be trapped and killed by a black light lamp. The anoplophora glabripennis can move around day and night, has weak phototaxis, and can be used for artificially catching imagoes. In winter, young forests can be combined to nurture and repair deinsectization branches, seriously damaged trees are cut down, and hidden dangers are eradicated. (3) The attractant is used for trapping and killing the longicorn adults. The oviposition inducing pheromone is used for inducing adult longicorn in the breeding stage, plant volatile matters and the insect pheromone have a synergistic effect, and the odor of the host plant can enhance the behavior reaction of the insect on the sex pheromone, the aggregation pheromone, the tracing pheromone, the alarm pheromone and the like. The intercropping attraction effect can be improved by applying a plant-source attractant and insect pheromone composite use technology. In addition, the composite use of the attractant and the pathogenic microorganisms of the longhorn such as beauveria bassiana (Beauveriavuillemin) and metarhizium anisopliae (Metarhizium anisopliae) can also improve the control effect. (4) Chemical measures are as follows: firstly, organic phosphorus pesticides such as zinc phosphide and the like can be made into poison sticks which are inserted into insect holes to fumigate and kill larvae. Secondly, 50 to 300 times of organophosphorus pesticide diluent is injected into the wormhole to kill the larva. Thirdly, the systemic pesticide such as monocrotophos, omethoate and the like is used for punching and injecting at the base part or the root part of the trunk, and the injected pesticide is sealed by wet soil, so that the larvas of tall trees can be prevented and treated. Fourthly, spraying 1000-2000 times of colloidal suspension of chlorbenzuron on a large area in the adult stage and the egg stage to kill the adult and the egg. Fifthly, using a novel contact insecticide 'green Weilei' to prevent and control longicorn adults.

The problems faced by the above methods are that the biological control effect is unstable, the control effect is affected by factors such as weather, and the longhorn beetles are used as boring pests, and the biological control agent hardly reaches adults and larvae of the longhorn beetles; chemical prevention methods such as using a poison tag, drilling injection, embedding medicine, spraying and the like are still used in large areas at present, but the methods have great problems in prevention effect or specific implementation operation. The dosage is large during spraying, fog drops can drift along with wind, a high-pressure spraying machine needs to be used, the pesticide cannot effectively reach the insect body, and the pesticide utilization rate is too low. The poison tagging method needs to find holes of longicorn larvae and imagoes, and the workload is too large when large-area prevention and control are carried out; the workload of drilling injection is too large during large-area prevention, the wood part of the tree is damaged after drilling, the self-healing capability of the tree is limited, most of the trees are in a mess, and farmers have certain difficulty in accepting the method. The targeted pesticide application technology has the characteristics of concentrated pesticide amount, less loss, high ecological selectivity, small influence on predatory property and parasitic natural enemies, high protection effect rate on tender branches and leaves newly grown out of plants after pesticide application, capability of killing pests hidden inside the plants, long residual effect period due to absorption and conduction effects, no influence from conditions such as external climate and the like, no environmental pollution and the like.

Disclosure of Invention

The invention aims to provide the ointment with the internal absorption and conduction effects for preventing and treating the longicorn, which is used for preventing and treating the longicorn insect pests, has long lasting period, small environmental pollution and simple and convenient application.

The invention aims to realize the technical scheme that the ointment with the internal absorption and conduction effects for preventing and treating the longicorn is prepared by mixing trifluorfluoropyrimidine, quinalphos and an auxiliary agent, wherein the trifluorfluoropyrimidine accounts for 0.1-20% of the total weight of the ointment, the quinalphos accounts for 0.1-20% of the total weight of the ointment, and the balance is the auxiliary agent.

Further, the weight ratio of the trifluoro-benzene pyrimidine to the quinalphos is 4:1, 2:1, 1:2 or 1: 4.

Further, the auxiliary agent comprises a solvent, an adhesive and a filler, wherein the solvent accounts for 0.1-80.0 wt% of the total weight of the ointment, the adhesive accounts for 0.1-80.0 wt% of the total weight of the ointment, and the filler accounts for 0.1-80.0 wt% of the total weight of the ointment.

Further, the solvent is selected from any one or a combination of any two or more of benzene, toluene, xylene, methanol, ethanol, propanol, butanol, isobutanol, cyclohexanone, methyl ethyl ketone, isophorone, pyrrolidone, N-methyl pyrrolidone, dimethylformamide, dimethyl sulfoxide and 2-pyrrolidone.

The adhesive is selected from one or a composition of any two or more of polyvinyl acetate, gelatin, polyvinyl alcohol, styrene butadiene latex, Arabic gum, sodium alginate, xanthan gum, sodium carboxymethylcellulose, sodium carboxypropylcellulose, polyethylene glycol, polyethylene oxide, polyvinylpyrrolidone, polyacrylamide, soluble starch, dextrin, polyvinyl acetate, coumarone resin, petroleum resin and naphthol-formaldehyde resin.

The filler is selected from one or a composition of any two or more of diatomite, attapulgite clay, bentonite, sepiolite, kaolin, pottery clay, light calcium carbonate, sawdust powder, rice hull, tobacco powder, walnut shell powder and lignin.

The application method of the ointment with the internal absorption and conduction effects for preventing and treating the longicorn comprises the following steps: and spirally stripping the bark from top to bottom around the trunk at a position on the trunk, which is 0.3-1.8 meters away from the root in a vertical manner, to form a spiral groove, wherein the width of the spiral groove is 2-20 centimeters, the depth of the spiral groove is up to the phloem of the trunk, the xylem of the trunk is leaked, and the ointment of the longicorn containing the trifluoro-phenylpyrimidine is coated at the position where the bark is stripped.

Compared with the prior art, the invention has the following beneficial effects:

firstly, the selected trifluoro-phenylpyrimidine and the quinalphos have good systemic effect, and the quinalphos has good contact killing effect. The prepared ointment is coated on the girdling part of a trunk, the medicament has good transportation and guiding effects on the phloem to reach the position where pests are harmful, particularly the position where boring pests are harmful, and meanwhile, the medicament with systemic and contact killing properties is coated on the girdling part of the trunk, so that the pests can be prevented from creeping back and forth from the bottom and the upper part of the trunk, and the opportunity of harm is greatly reduced;

secondly, after the trifluoro-fluoropyrimidine with good systemic property and the quinalphos are mixed, the solvent, the adhesive, the filler and the like are added according to the proportion to prepare the ointment, and the ointment is canned for forming; before the ointment is used, a self-made annular peeling knife is utilized to spirally peel a groove with the width of about 2-20 cm from top to bottom around a trunk at the position of 0.3-1.8 m of the trunk, only the peeled phloem is cut without damaging the xylem, the xylem leaks out (the width of the peeled bark and the vertical distance between an upper point and a lower point are changed due to the diameter change of the tree), the prepared ointment is coated on the peeled bark position, the ointment is conducted to the position where pests are harmful through plants, the duration of the ointment is long, the environmental pollution is small, the operation is simple and convenient, the bark is only required to be slightly peeled, the prepared ointment is coated on the peeled position through an extrusion canning device, and the bark peeling and the application can be continuously carried out. Meanwhile, the ointment used at the stripping part has a strong contact action, and a barrier with a certain width is formed between the upper part and the lower part of the trunk to make a round trip, so that when pests crawl on the trunk, the pests can be killed after contacting the ointment, and the damage of the crawling pests to plants is greatly reduced.

Thirdly, the ointment is applied to the bark peeled part, has good adhesive force and lasting effect, is simple and convenient to apply, improves the utilization rate of the medicament, reduces the environmental pollution, can enter plants through the absorption of the plant epidermis, has certain contact killing effect, can prevent pests from crawling up and down on the trunk, and thus can greatly reduce the damage to the plants.

Detailed Description

The following examples are further illustrative of the present invention but are not intended to limit the scope of the invention (all compositions in the following examples are in weight percent).

Example 1, 12.5% Trifluoropyrimidine Quihon ointment

10.0 parts of trifluoro-benzene pyrimidine, 2.5 parts of quinalphos, 4.0 parts of xylene, 5.0 parts of propanol, 2.0 parts of polyvinyl acetate, 2.0 parts of Arabic gum, 2.0 parts of gelatin, 15.0 parts of light carbonic acid and 57.5 parts of diatomite; mixing the binder, the filler and the solvent, adding the pesticide active ingredient under stirring at normal temperature to form paste, and canning.

Example 2, 15.0% Trifluoropyrimidine Quihon ointment

10.0 parts of trifluoro-benzene pyrimidine, 5.0 parts of quinalphos, 5.0 parts of pyrrolidone, 5.0 parts of methyl ethyl ketone, 4.0 parts of dimethylformamide, 6.0 parts of coumarone resin, 10.0 parts of sawdust powder and 55.0 parts of attapulgite clay; mixing the binder, the filler and the solvent, adding the pesticide active ingredient under stirring at normal temperature to form paste, and canning.

Example 3, 20.0% Trifluoropyrimidine Quihon ointment

10.0 parts of trifluoro-benzene pyrimidine, 10.0 parts of quinalphos, 6.0 parts of polyethylene oxide, 8.0 parts of 2-pyrrolidone, 4.0 parts of dextrin, 4.0 parts of coumarone resin, 6.0 parts of lignin and 52.0 parts of kaolin; mixing the binder, the filler and the solvent, adding the pesticide active ingredient under stirring at normal temperature to form paste, and canning.

Example 4, 15.0% Trifluoropyrimidine Quihon ointment

5.0 parts of trifluoro-benzene pyrimidine, 10.0 parts of quinalphos, 4.0 parts of methyl ethyl ketone, 4.0 parts of dimethyl sulfoxide, 2.0 parts of 2-pyrrolidone, 5.0 parts of sodium carboxymethyl cellulose, 8.0 parts of sodium alginate, 4.0 parts of xanthan gum, 18.0 parts of light calcium carbonate and 40.0 parts of lignin; mixing the binder, the filler and the solvent, adding the pesticide active ingredient under stirring at normal temperature to form paste, and canning.

Example 5, 12.5% Trifluoropyrimidine Quihon ointment

2.5 parts of trifluoro-benzene pyrimidine, 10.0 parts of quinalphos, 4.0 parts of butanol, 10.0 parts of isophorone, 5.0 parts of methyl ethyl ketone, 12.0 parts of sodium carboxymethyl cellulose, 3.0 parts of gelatin, 5.0 parts of tobacco powder, 6.0 parts of rice husk and 42.5 parts of pottery clay; mixing the binder, the filler and the solvent, adding the pesticide active ingredient under stirring at normal temperature to form paste, and canning.

Example 6, 20.1% Trifluoropyrimidine Quihon ointment

20 parts of trifluoro-benzene pyrimidine, 0.1 part of quinalphos, 5.0 parts of dimethylformamide, 8.0 parts of methanol, 5.0 parts of sodium carboxypropyl cellulose, 8.0 parts of soluble starch, 40.0 parts of coumarone resin and 13.9 parts of kaolin; mixing the binder, the filler and the solvent, adding the pesticide active ingredient under stirring at normal temperature to form paste, and canning.

Example 7, 20.1% Trifluoropyrimidine Quihon ointment

0.1 part of trifluoro-benzene pyrimidine, 20.0 parts of quinalphos, 6.0 parts of isophorone, 8.0 parts of pyrrolidone, 6.0 parts of polyoxyethylene, 8.0 parts of naphthol-formaldehyde resin, 5.0 parts of bentonite, 10.0 parts of attapulgite clay and 36.9 parts of sepiolite; mixing the binder, the filler and the solvent, adding the pesticide active ingredient under stirring at normal temperature to form paste, and canning.

Example 8, 40.0% Trifluoropyrimidine Quihon ointment

20 parts of trifluoro-benzene pyrimidine, 20.0 parts of quinalphos, 8.0 parts of dimethyl sulfoxide, 6.0 parts of isophorone, 6.0 parts of pyrrolidone, 6.0 parts of polyoxyethylene, 8.0 parts of naphthol-formaldehyde resin, 16.0 parts of pottery clay and 10 parts of attapulgite clay; mixing the binder, the filler and the solvent, adding the pesticide active ingredient under stirring at normal temperature to form paste, and canning.

Example 9: indoor mixed agent synergy toxicity determination

Respectively preparing each reagent to be tested into corresponding concentration, sucking the prepared pesticide from low concentration to high concentration by a micro-injector, dripping the pesticide on the forebreast back plate of 3-year larva of anoplophora glabripennis, washing the micro-injector by acetone among different medicaments, dripping 1 mu L of the pesticide on each end, using 4% acetone solution as a contrast at each concentration of 30 ends, observing the death number of the larva within 24 hours, and calculating LC₅₀Values, regression equation for virulence and co-virulence coefficients. The cotoxicity coefficient is calculated by the Sun Yunpei method. When the co-toxicity coefficient is obviously more than 100, the synergistic effect is shown; near 100, indicating additive effect; obviously less than 100, indicating antagonism. The calculation results are shown in Table 1. Co-toxicity coefficient CTC ═ 100 × virulence index of actual mixture/virulence index of theoretical mixtureAnd (4) counting.

TABLE 1 determination of toxicity of each indoor agent to anoplophora glabripennis

Reagent for testing	Regression equation of virulence	LC₅₀Value (μ g/mg)	Correlation coefficient	Co-toxicity coefficient
					Trifluorophenylpyrimidines	y＝2.8118+1.5886x	23.85	0.9815
Quinalphos	y＝2.1644+1.6197x	56.33	0.9748
					Trifluoropyrimidine quinalphos 4:1	y＝2.1889+2.0668x	22.92	0.9829	117.64
Trifluoropyrimidine quinalphos 2:1	y＝1.9055+2.2717x	23.02	0.9636	128.23
					1:1 ratio of trifluoro-phenylpyrimidine to quinalphos	y＝2.2348+1.9877x	24.61	0.9877	136.16
1:2 ratio of trifluoro-benzene pyrimidine quinalphos	y＝2.4607+1.8142x	25.10	0.9725	154.33
					1:4 of trifluoro-phenylpyrimidine quinalphos	y＝1.9014+2.0776x	31.00	0.9869	142.80

As can be seen from Table 1, the respective proportions of trifluorobenzene pyrimidine, quinalphos, trifluorobenzene pyrimidine and quinalphos are mixed to form the effective lethal medium concentration LC of anoplophora glabripennis₅₀The values in sequence (4:1, 2:1, 1:2 and 1:4) are 23.85, 56.33, 22.92, 23.02, 24.61, 25.10 and 31.00. mu.g/mL, respectively; the co-toxicity coefficients of the mixture of the trifluoro-phenylpyrimidine and the quinalphos are 117.64, 128.23, 136.16, 154.33 and 142.80 in sequence (4:1, 2:1, 1:2 and 1: 4). The proportion of the trifluoro-benzene pyrimidine and the quinalphos is2:1, 1:2 and 1:4 have synergistic effect, wherein the synergistic effect is most obvious when the proportion is 1: 2.

Example 10: test result of field drug effect

Test site: the growing conditions and the management conditions of willow in Siyang county of Jiangsu are consistent, and the anoplophora glabripennis in the growing area is serious. Test time: 7-8 months in 2018, weather conditions: the test period is all sunny, the temperature is 29-37 ℃, the humidity is 70%, and no other abnormal weather exists. The test area is 5 meters of willow, the diameter is 30 centimeters, the growth and management conditions are consistent, the trunk is spirally and circularly cut by a cutter at a position which is vertical to the root of the willow and is about 1.5 meters away from the root of the willow, the bark of the willow is removed from top to bottom, the bark is spirally stripped from top to bottom (without opening), a spiral groove is formed, the vertical distance between the stripping starting point and the terminal point is 50 centimeters, and the width of the groove is 4 centimeters. The prepared ointment of examples 1 to 8, 20 percent of trifluoropyrimidine ointment and 20 percent of quinalphos single-dose ointment are uniformly coated on the cut groove part, the death condition of anoplophora glabripennis in the trunk is investigated in 35 days, and the death rate and the prevention and treatment effect are calculated. The results are shown in Table 2:

TABLE 2 Effect of each preparation on preventing and treating anoplophora glabripennis

Treatment of	Control effect (%) 35 days after administration
		Control	-18.46±2.15
20% trifluoro-benzene pyrimidine ointment	78.13±1.67
		20% quinalphos ointment	72.86±1.25
Example 1	88.51±1.88
		Example 2	87.57±2.25
Example 3	93.56±3.17
		Example 4	91.18±1.29
Example 5	89.49±2.14
		Example 6	96.18±1.98
Example 7	92.17±2.63
		Example 8	100.00±0

Claims

1. An ointment with a systemic conduction function for preventing and treating longicorn is characterized in that: the ointment is prepared by mixing trifluorfluoropyrimidine, quinalphos and an auxiliary agent, wherein the weight percentage of the trifluorfluoropyrimidine to the total weight of the ointment is 0.1-20%, the weight percentage of the quinalphos to the total weight of the ointment is 0.1-20%, and the balance of the auxiliary agent.

2. The ointment for preventing and treating longicorn with systemic conduction effect according to claim 1, is characterized in that: the weight ratio of the trifluoro-benzene pyrimidine to the quinalphos is 4:1, 2:1, 1:2 or 1: 4.

3. The ointment for preventing and treating longicorn with systemic conduction effect according to claim 1, is characterized in that: the auxiliary agent comprises a solvent, an adhesive and a filler, wherein the solvent accounts for 0.1-80.0 wt% of the total weight of the ointment, the adhesive accounts for 0.1-80.0 wt% of the total weight of the ointment, and the filler accounts for 0.1-80.0 wt% of the total weight of the ointment.

4. The ointment for preventing and treating longicorn with systemic conduction effect according to claim 3, is characterized in that: the solvent is selected from any one or a composition of more than two of benzene, toluene, xylene, methanol, ethanol, propanol, butanol, isobutanol, cyclohexanone, methyl ethyl ketone, isophorone, pyrrolidone, N-methyl pyrrolidone, dimethylformamide, dimethyl sulfoxide and 2-pyrrolidone.

5. The ointment for preventing and treating longicorn with systemic conduction effect according to claim 3, is characterized in that: the adhesive is selected from one or more of polyvinyl acetate, gelatin, polyvinyl alcohol, styrene butadiene latex, Arabic gum, sodium alginate, xanthan gum, sodium carboxymethylcellulose, sodium carboxypropylcellulose, polyethylene glycol, polyethylene oxide, polyvinylpyrrolidone, polyacrylamide, soluble starch, dextrin, polyvinyl acetate, coumarone resin, petroleum resin and naphthol-formaldehyde resin.

6. The ointment for preventing and treating longicorn with systemic conduction effect according to claim 3, is characterized in that: the filler is selected from one or more of diatomite, attapulgite clay, bentonite, sepiolite, kaolin, pottery clay, light calcium carbonate, sawdust powder, rice hull, tobacco powder, walnut shell powder and lignin.

7. The use method of the ointment for preventing and treating longicorn with systemic conduction as claimed in any one of claims 1 to 6, is characterized in that: and spirally stripping off barks from top to bottom around the trunk at a position on the trunk, which is 0.3-1.8 meters away from the root in a vertical manner, to form a spiral groove, wherein the width of the spiral groove is 2-20 centimeters, the depth of the spiral groove is up to the phloem of the trunk, the xylem of the trunk is leaked, and the ointment with the internal absorption and conduction effect is coated at the position of stripping off the barks.