CN109251863A - A kind of novel cell culture vessel - Google Patents
A kind of novel cell culture vessel Download PDFInfo
- Publication number
- CN109251863A CN109251863A CN201811397348.3A CN201811397348A CN109251863A CN 109251863 A CN109251863 A CN 109251863A CN 201811397348 A CN201811397348 A CN 201811397348A CN 109251863 A CN109251863 A CN 109251863A
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- CN
- China
- Prior art keywords
- mould group
- pedestal
- cell culture
- culture vessel
- fixedly installed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000004113 cell culture Methods 0.000 title claims abstract description 24
- 230000007246 mechanism Effects 0.000 claims abstract description 55
- 239000007788 liquid Substances 0.000 claims abstract description 46
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000012530 fluid Substances 0.000 claims abstract description 10
- 230000005540 biological transmission Effects 0.000 claims abstract description 8
- 238000009434 installation Methods 0.000 claims description 2
- 238000003466 welding Methods 0.000 claims description 2
- 230000009471 action Effects 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000004886 process control Methods 0.000 description 3
- 230000003749 cleanliness Effects 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/40—Manifolds; Distribution pieces
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of cell culture vessels, in particular a kind of novel cell culture vessel, including seal closure, full-automatic liquid relief fluid body, pedestal, it automatically uncaps and closes lid mechanism and mounting base, through-hole is offered at the top of the pedestal, the inside of the through-hole is provided through with liquid storehouse, it is described to be fixedly installed with motor rotating mechanism with liquid orlop portion, the motor rotating mechanism is fixedly connected with liquid storehouse by rotating seal ring, the motor rotating mechanism and pedestal are fixedly mounted by mounting base, it is described to be fixedly installed with dial feed structure with liquid the top of the warehouse, hollow design is provided on the dial feed structure, pedestal is fixedly installed at the top of the pedestal, it is provided with automatically to uncap at the top of the pedestal and closes lid mechanism, it is described automatically to uncap that close lid mechanism include transmission parts, third mould group, second mould group and One mould group, single unit system structure of the present invention is simple, and work efficiency is high, reaches GMP and closes rule, has certain popularizing action.
Description
Technical field
The present invention relates to a kind of cell culture vessel, specially a kind of novel cell culture vessel.
Background technique
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology, no matter for
For entire biotechnology, or one of them biological cloning technology, cell culture is all an essential mistake
Journey, the extensive clone of cell culture inherently cell, cell culture technology can by a cell by mass propgation at
For simple many cells that are unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture itself
It is exactly the clone of cell, a large amount of cell or its metabolite is obtained by cell culture, because biological product is all from cell
It gets, it is possible to say that cell culture technology is most crucial, most basic technology in biotechnology, and existing cell training at present
Feeding operating with liquid is to match liquid with liquid or lower than the GMP automation/semi-automation for closing rule by hand, but there are low efficiency, consumptions
When, high labor cost and it is unable to reach the defect that GMP closes rule, it is therefore desirable to which a kind of novel cell culture vessel is to the above problem
Make improvement.
Summary of the invention
It is mentioned above in the background art to solve the purpose of the present invention is to provide a kind of novel cell culture vessel
Problem.
To achieve the above object, the invention provides the following technical scheme:
A kind of novel cell culture vessel, including seal closure, full-automatic liquid relief fluid body, pedestal, automatically uncap
Lid mechanism and mounting base are closed, offers through-hole on the pedestal top, the inside of the through-hole is provided through with liquid storehouse, described
Motor rotating mechanism is fixedly installed with liquid orlop portion, the motor rotating mechanism and passes through the fixed company of rotating seal ring with liquid storehouse
It connects, the motor rotating mechanism and pedestal are fixedly mounted by mounting base, described to be fixedly installed with turntable feeding with liquid the top of the warehouse
Mechanism is provided with hollow design on the dial feed structure, pedestal is fixedly installed at the top of the pedestal, the pedestal
Top, which is provided with, automatically uncaps and closes lid mechanism, described automatically to uncap that close lid mechanism include transmission parts, third mould group, the
Two mould groups and the first mould group, the transmission parts, third mould group, the second mould group, the first mould group and pedestal are run through by fixing axle
Connection, the top of the fixing axle are fixedly installed with Z axis motor, and the third mould group, the second mould group and the first mould group are by twisting
For mandrel through connection, the top of the tightening axle, which is fixedly installed with, tightens motor, and the first mould group and dial feed structure are logical
Cross culture vessel flexible connection, described automatically uncap closes the periphery of lid mechanism and be arranged with seal closure, the back side of the seal closure
It is fixedly installed with full-automatic liquid relief fluid body.
Preferably, the turntable is in Pentagram shape.
Preferably, the fixing axle is provided with three groups, and size is equal.
Preferably, the pedestal is fixedly connected with mounting base by electric welding.
Preferably, the inner diameter size of the rotating seal ring and outer diameter with liquid storehouse are equal in magnitude.
Preferably, perforation is offered in the first mould group, the second mould group and third mould group.
Compared with prior art, the beneficial effects of the present invention are:
1, in the present invention, in use, the motor rotating mechanism by setting is started to work, and the turntable feeding of setting is driven
Mechanism starts turning, and closes lid mechanism bottom so that the culture vessel of setting is moved to automatically to uncap and carries out operation of uncapping,
And the full-automatic liquid relief fluid body bottom for being moved to setting carries out liquid relief to it and operates with liquid, then is moved to the full-automatic of setting
Uncap close lid mechanism bottom carry out close lid operation, by this operating process solve current automation/semi-automation match liquid can not
Reach GMP and close rule problem, and work efficiency is high, cost of labor is low.
2, in the present invention, in use, driving the first mould closed in lid mechanism of automatically uncapping by process control Z axis motor
Group, the second mould group and third mould group move up and down to operating position, tighten motor and drive tightening axle switch bottle cap, reach and open automatically
Close the purpose of lid, and by be set in automatically uncap close the seal closure of lid mechanism periphery the parts such as motor, cable seal every
From, while in the enterprising action sealing of whole axis, achieve the purpose that protect part and guarantee with liquid storehouse cleanliness.
3, in the present invention, in use, the dial feed structure by setting uses standardized designs, same station can be adapted to
Multiple types consumptive material, and the hollow design by being arranged on dial feed structure alleviates the weight of overall mechanism itself, and
The permeability of gas, while the drive part motor rotating mechanism of the dial feed structure by setting are increased, ensure that fortune
Dynamic precision, while ensure that the leakproofness with liquid storehouse by driving it the design of underlying and rotating seal ring.
4, in the present invention, in use, increasing the stability of single unit system by the pedestal being arranged, and pass through the peace of setting
Dress seat ensure that an integrality of motor-driven mechanism and single unit system, and the pedestal by being arranged makes the fixing axle of setting
With a supporting point, while it being made to reach a connection relationship with pedestal, closes lid mechanism to increase and automatically uncap
Stability.
Detailed description of the invention
Fig. 1 is integral outer structural schematic diagram of the present invention；
Fig. 3 is that full-automatic uncap of the present invention closes lid mechanism structural schematic diagram；
Fig. 2 is A partial enlargement diagram of the present invention；
In figure: the full-automatic liquid relief fluid body of 1- seal closure, 2-, 3- pedestal, 4-, which automatically uncap, closes lid mechanism, 5- installation
Seat, 6- pedestal, 7- fixing axle, the first mould of 8- group, 9- culture vessel, 10- dial feed structure, 11- hollow design, 12- transmission
Component, 13- tightening axle, the second mould of 14- group, 15- third mould group, 16- tighten motor, 17-Z spindle motor, 18- with liquid storehouse, 19- electricity
Machine rotating mechanism, 20- through-hole, 21- rotating seal ring.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1-3 is please referred to, the present invention provides a kind of technical solution:
A kind of novel cell culture vessel, including seal closure 1, full-automatic liquid relief fluid body 2, pedestal 3, full-automatic
Lid closes lid mechanism 4 and mounting base 5, offers through-hole 20 on 3 top of pedestal, the inside of the through-hole 20, which is provided through, matches
Liquid storehouse 18, it is described to be fixedly installed with motor rotating mechanism 19 with 18 bottom of liquid storehouse, the motor rotating mechanism 19 and match liquid storehouse 18
It is fixedly connected by rotating seal ring 21, the motor rotating mechanism 19 and pedestal 3 are fixedly mounted by mounting base 5, described to match
It is fixedly installed with dial feed structure 10 at the top of liquid storehouse 18, hollow design 11 is provided on the dial feed structure 10, uses
When, standardized designs are used by the dial feed structure 10 of setting, same station can be adapted to multiple types consumptive material, and by setting
The hollow design 11 set on dial feed structure 10 alleviates the weight of overall mechanism itself, and increases the penetrating of gas
Property, while the drive part motor rotating mechanism 19 of the dial feed structure 10 by setting, it ensure that the precision of movement, simultaneously
It ensure that the leakproofness with liquid storehouse 18 by driving it the design of underlying and rotating seal ring 21, the top of the pedestal 3 is solid
Dingan County is equipped with pedestal 6, and the top of the pedestal 6, which is provided with automatically to uncap, closes lid mechanism 4, and described automatically uncap closes lid mechanism
4 include transmission parts 12, third mould group 15, the second mould group 14 and the first mould group 8, the transmission parts 12, third mould group 15,
Second mould group 14, the first mould group 8 and pedestal 6 run through connection by fixing axle 7, in use, being increased by the pedestal 3 of setting whole
The stability of body device, and the mounting base 5 by being arranged ensure that an integrality of motor-driven mechanism 19 Yu single unit system,
And make the fixing axle 7 of setting that there is a supporting point by the pedestal 6 of setting, while it being made to reach a company with pedestal 3
Relationship is connect, closes the stability of lid mechanism 4 to increase and automatically uncap, the top of the fixing axle is fixedly installed with Z axis motor
17, the third mould group 15, the second mould group 14 and the first mould group 8 are by tightening axle 13 through connection, the top of the tightening axle 13
End, which is fixedly installed with, tightens motor 16, and the first mould group 8 and dial feed structure 10 are flexibly connected by culture vessel 9, institute
State automatically to uncap and close the periphery of lid mechanism 4 and be arranged with seal closure 1, in use, by process control Z axis motor 17 drive it is complete from
Dynamic the first mould group 8 closed in lid mechanism 4 of uncapping, the second mould group 14 and third mould group 15 move up and down to operating position, tighten electricity
Machine 16 drives tightening axle 13 to switch bottle cap, achievees the purpose that automatically opening and closing, and close lid mechanism 4 by being set in automatically to uncap
The seal closure 1 of periphery seals off the parts such as motor, cable, while in the enterprising action sealing of whole axis, reaching protection part
With the purpose for guaranteeing to match 18 cleanliness of liquid storehouse, the back side of the seal closure 1 is fixedly installed with full-automatic liquid relief fluid body 2, makes
Used time is started to work by the motor rotating mechanism 19 of setting, and the dial feed structure 10 of setting is driven to start turning, thus
It closes 4 bottom of lid mechanism so that the culture vessel 9 of setting is moved to automatically to uncap and carries out operation of uncapping, and be moved to the complete of setting
Automatic 2 bottom of liquid relief fluid body carries out liquid relief to it and operates with liquid, then is moved to automatically uncapping for setting and closes 4 bottom of lid mechanism
Portion carry out close lid operation, by this operating process solve current automation/semi-automation with liquid be unable to reach GMP close advise ask
Topic, and work efficiency is high, cost of labor is low.
Working principle of the present invention: in use, the motor rotating mechanism 19 by setting is started to work, and turning for setting is driven
Disk feeding mechanism 10 starts turning, so that the culture vessel 9 of setting, which is moved to automatically to uncap, closes 4 bottom of lid mechanism, leads to
Crossing process control Z axis motor 17 drives the first mould group 8, the second mould group 14 and third mould group 15 of setting to move up and down to working position
It sets, and the motor 16 of tightening by being arranged drives tightening axle 13 to carry out operation of uncapping, and the full-automatic liquid relief for being moved to setting is matched
2 bottom of liquid mechanism carries out liquid relief to it and operates with liquid, then is moved to automatically uncapping for setting and closes 4 bottom of lid mechanism and carry out pass lid
Operation, in cycles, single unit system structure is simple, and work efficiency is high, and cost of labor is low, reaches GMP and closes rule, has certain push away
Wide value.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of novel cell culture vessel, including seal closure (1), full-automatic liquid relief fluid body (2), pedestal (3), it is complete from
Dynamic uncap closes lid mechanism (4) and mounting base (5), it is characterised in that: it is offered through-hole (20) at the top of the pedestal (3), it is described
The inside of through-hole (20) is provided through with liquid storehouse (18), described to be fixedly installed with motor rotating mechanism with liquid storehouse (18) bottom
(19), the motor rotating mechanism (19) is fixedly connected with liquid storehouse (18) by rotating seal ring (21), the motor rotation
Mechanism (19) and pedestal (3) are fixedly mounted by mounting base (5), described to be fixedly installed with dial feed with liquid storehouse (18) top
Structure (10) is provided with hollow design (11) on the dial feed structure (10), is fixedly installed with bottom at the top of the pedestal (3)
Seat (6) is provided at the top of the pedestal (6) automatically to uncap and close lid mechanism (4), and described automatically uncap closes lid mechanism (4)
It include transmission parts (12), third mould group (15), the second mould group (14) and the first mould group (8), the transmission parts (12),
Three mould groups (15), the second mould group (14), the first mould group (8) and pedestal (6) are by fixing axle (7) through connection, the fixing axle
Top be fixedly installed with Z axis motor (17), the third mould group (15), the second mould group (14) and the first mould group (8) by twist
Mandrel (13) through connection, the top of the tightening axle (13), which is fixedly installed with, tightens motor (16), the first mould group (8) and
Dial feed structure (10) is flexibly connected by culture vessel (9), and described automatically uncap is closed the periphery of lid mechanism (4) and be arranged with
Seal closure (1), the back side of the seal closure (1) are fixedly installed with full-automatic liquid relief fluid body (2).
2. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: the turntable (10) is in five jiaos
Starriness.
3. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: fixing axle (7) setting
There are three groups, and size is equal.
4. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: the pedestal (3) and installation
Seat (5) is fixedly connected by electric welding.
5. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: the rotating seal ring (21)
Inner diameter size and with liquid storehouse (18) outer diameter it is equal in magnitude.
6. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: the first mould group (8),
Perforation is offered in two mould groups (14) and third mould group (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811397348.3A CN109251863A (en) | 2018-11-22 | 2018-11-22 | A kind of novel cell culture vessel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811397348.3A CN109251863A (en) | 2018-11-22 | 2018-11-22 | A kind of novel cell culture vessel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109251863A true CN109251863A (en) | 2019-01-22 |
Family
ID=65042020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811397348.3A Pending CN109251863A (en) | 2018-11-22 | 2018-11-22 | A kind of novel cell culture vessel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109251863A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110252430A (en) * | 2019-07-02 | 2019-09-20 | 英诺维尔智能科技（苏州）有限公司 | A kind of full-service fluid operating platform |
| CN110511872A (en) * | 2019-09-29 | 2019-11-29 | 深圳赛动生物自动化有限公司 | Cell batch automatic production equipment and its working method |
| CN110538592A (en) * | 2019-09-29 | 2019-12-06 | 深圳赛动生物自动化有限公司 | Automatic liquid preparation device and working method thereof |
| CN117947138A (en) * | 2024-03-27 | 2024-04-30 | 佳木斯大学 | In-vitro cytotoxicity test method for oral cavity material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110252430A (en) * | 2019-07-02 | 2019-09-20 | 英诺维尔智能科技（苏州）有限公司 | A kind of full-service fluid operating platform |
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| CN110511872A (en) * | 2019-09-29 | 2019-11-29 | 深圳赛动生物自动化有限公司 | Cell batch automatic production equipment and its working method |
| CN110538592A (en) * | 2019-09-29 | 2019-12-06 | 深圳赛动生物自动化有限公司 | Automatic liquid preparation device and working method thereof |
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| CN110538592B (en) * | 2019-09-29 | 2024-03-29 | 深圳赛动生物自动化有限公司 | Automatic liquid preparation device and working method thereof |
| CN117947138A (en) * | 2024-03-27 | 2024-04-30 | 佳木斯大学 | In-vitro cytotoxicity test method for oral cavity material |
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