CN109251857A - Laser microprobe dating instrument and its working method - Google Patents
Laser microprobe dating instrument and its working method Download PDFInfo
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- CN109251857A CN109251857A CN201811297289.2A CN201811297289A CN109251857A CN 109251857 A CN109251857 A CN 109251857A CN 201811297289 A CN201811297289 A CN 201811297289A CN 109251857 A CN109251857 A CN 109251857A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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Abstract
The present invention provides a kind of laser microprobe dating instrument and its working method, laser microprobe dating instrument includes laser, object lens；Object lens include: the optical channel having in shell along casing center axis；Laser is arranged in shell, and the wavelength of the rupture of membranes light of outgoing is λ1,Laser is 0 perpendicular to the projected area on the section on the central axis the optical channel；Wavelength convert component is arranged in the shell, and in the emergent light optical path in the laser；The wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2∈[380nm,780nm]；Filter switch, and the optical filtering switch is λ for preventing wavelength as needed1Rupture of membranes light emission to embryo；Rupture of membranes light and/or conversion light are reflected into reflective mirror by two-dimensional scanning mirror unit；The projected area of two-dimensional scanning mirror unit and wavelength convert component on the section is 0；Reflective mirror is arranged in the optical channel；Reflected light on reflective mirror is transmitted in the optical channel.The present invention, which has, controls accurate, advantages of simple structure and simple.
Description
Technical field
The present invention relates to Embryo Cultures, in particular to laser microprobe dating instrument and its working method.
Background technique
Laser microprobe dating instrument is a kind of equipment for being scanned cutting operation to embryo by emission pulse laser beam.Swash
In light rupture of membranes technology, the design to laser and instruction light is a very crucial job.Since laser is usually infrared light, people
Eye it is invisible, indicate that the design of light can help operator that the position of laser beam is accurately positioned, if instruction light with
The positional relationship processing of laser is bad, then beam of laser energy can be made to get to other positions, cause to damage to embryo.It is existing at present
Scheme is main are as follows:
The first scheme: laser and instruction light are individually placed to object lens two sides, a face reflecting mirror and object are put among object lens
The central axis of mirror fixation at a certain angle, the laser that laser issues, is incident on after being incident on the head-on reflection of reflecting mirror
Object lens, guidance light are incident on eyepiece after being incident on the reverse side reflection of reflecting mirror.Guide light and laser all in the central axis of object lens
On, it is contrary.The deficiency of this method is:
1. needing to use two light source variables, the difficulty and error of adjustment will increase in the process of adjustment, it is not easily-controllable
System.
2. can only carry out laser single-point shooting, if to realize cutting must by way of manual moving stage,
It cannot achieve automatically scanning cutting.
Second scheme: scanning system is mounted on the fluorescence channel of inverted microscope, and deficiency is: occupying fluorescence function
It can use, it is very inconvenient.
Summary of the invention
To solve the deficiency in above-mentioned prior art, the present invention provides a kind of laser microprobe dating instrument, realize
Two-dimensional scanning, and it is not take up fluorescence channel.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of laser microprobe dating instrument, the laser microprobe dating instrument include laser, object lens；The object lens
Include:
Shell, the interior optical channel having along the casing center axis of the shell；The laser is arranged in shell
In vivo, the wavelength of the rupture of membranes light of outgoing is λ1,The laser the optical channel perpendicular to
The projected area on section on the central axis is 0；
Wavelength convert component, the wavelength convert component are arranged in the shell, and are in the outgoing of the laser
In light optical path；The wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2∈[380nm,
780nm]；
Filter switch, and the optical filtering switch is λ for preventing wavelength as needed1Rupture of membranes light emission to embryo；
Rupture of membranes light and/or conversion light are reflected into reflective mirror by two-dimensional scanning mirror unit, the two-dimensional scanning mirror unit；It is described
The projected area of two-dimensional scanning mirror unit and wavelength convert component on the section is 0；
Reflective mirror, the reflective mirror are arranged in the optical channel；Reflected light on reflective mirror is in the optical channel
Interior transmission.
According to above-mentioned laser microprobe dating instrument, it is preferable that the reflective mirror is greater than the reflectivity of the rupture of membranes light
90%, which is greater than the reflectivity to the conversion light.
According to above-mentioned laser microprobe dating instrument, it is preferable that the reflective mirror is to wavelength X3The reflectivity of light be less than
1%, λ3∈[380nm,λ)2∪(λ2, 780nm].
According to above-mentioned laser microprobe dating instrument, optionally, the object lens further comprise:
Mirror unit, the mirror unit are arranged in the shell, and the conversion light is reflected into two-dimensional scanning
Mirror unit；Projected area of the mirror unit on the section is 0.
According to above-mentioned laser microprobe dating instrument, optionally, the mirror unit includes:
First reflecting mirror, the emergent light are successively reflected by the first reflecting mirror and the second reflecting mirror；
Second reflecting mirror, first reflecting mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel.
According to above-mentioned laser microprobe dating instrument, optionally, the two-dimensional scanning mirror unit includes:
First scanning mirror, first scanning mirror and the second scanning mirror are setting up and down；
The rotary shaft of second scanning mirror, the first scanning mirror and the second scanning mirror is mutually perpendicular to.
According to above-mentioned laser microprobe dating instrument, it is preferable that the wavelength convert component is frequency multiplication component.
According to above-mentioned laser microprobe dating instrument, it is preferable that wavelength X1, λ1∈[1400nm,1500nm]。
The object of the invention is also to provide the working method of above-mentioned laser microprobe dating instrument, which is logical
Cross what following technical scheme was achieved:
According to the working method of above-mentioned laser microprobe dating instrument, the working method the following steps are included:
(A1) the rupture of membranes light that laser issues is incident on wavelength convert component；
(A2) wavelength convert component works, by the wavelength X of the rupture of membranes light1It is transformed into wavelength X2∈[380nm,780nm]；
(A3) conversion light is mapped on embryo after two-dimensional scanning mirror unit, reflective mirror；
Two-dimensional scanning mirror unit is adjusted, so that conversion light is mapped to the designated position of embryo；Wavelength X1Rupture of membranes light be prevented from
It is mapped on embryo；
(A4) working condition of wavelength convert component is adjusted, the rupture of membranes light that laser issues successively passes through the two-dimensional scanning
The designated position of embryo is mapped to after mirror unit and reflective mirror.
According to above-mentioned working method, optionally, in step (A4), the rupture of membranes light passes through the wavelength convert component
When, small part rupture of membranes light is converted to conversion light, and most of rupture of membranes optical wavelength unchangeably passes through wavelength convert component；Rupture of membranes light and turn
Change the designated position that light is mapped to embryo.
Compared with prior art, the device have the advantages that are as follows:
1. object lens have both laser two-dimension scanning function, accurate positioning；
2. each component and optical path are not take up fluorescence channel in use process.
Detailed description of the invention
Referring to attached drawing, the disclosure of the present invention will become more readily understood.Skilled addressee readily understands that: this
It is only used to illustrate the technical scheme of the present invention for a little attached drawings, and is not intended to limit the scope of protection of the present invention.
In figure:
Fig. 1 is the structure diagram of according to embodiments of the present invention 1 laser microprobe dating instrument；
Fig. 2 is the structure diagram of according to embodiments of the present invention 2 laser microprobe dating instrument.
Specific embodiment
Fig. 1-2 and following description describe optional embodiments of the invention to instruct how those skilled in the art implement
It is of the invention with reproducing.In order to teach the technical scheme of the invention, it has simplified or omitted some conventional aspects.Those skilled in the art
It should be understood that the variation or replacement from these embodiments will within the scope of the invention.Those skilled in the art should understand that
The following features can be combined in various ways to form multiple variations of the present invention.As a result, the invention is not limited to it is following can
Embodiment is selected, and is only limited by the claims and their equivalents.
Embodiment 1:
Fig. 1 schematically illustrates the structure diagram of the laser microprobe dating instrument of the embodiment of the present invention 1, as shown in Figure 1, institute
Stating laser microprobe dating instrument includes:
Laser, the wavelength for the rupture of membranes light that the laser issues are λ1, λ1∈ [1400nm, 1500nm],
Object lens, the object lens include:
Shell, the interior optical channel having along the casing center axis of the shell；The laser is arranged in shell
In vivo, the laser is 0 perpendicular to projected area on the section on the central axis in the optical channel；
Wavelength convert component, such as frequency multiplication component, the wavelength convert component is arranged in the shell, and swashs in described
In the emergent light optical path of light device；The wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2
∈[380nm,780nm]；The transfer efficiency of wavelength convert component is adjustable；
Filter switch, and it is λ that the optical filtering switch filters out wavelength as needed1Rupture of membranes light；
Two-dimensional scanning mirror unit, such as MEMS scanning galvanometer, the two-dimensional scanning mirror unit is anti-by rupture of membranes light and/or conversion light
It is mapped to reflective mirror；The projected area of the two-dimensional scanning mirror unit and wavelength convert component on the section is 0, and laser
Optical path and optical channel between device, wavelength convert component and two-dimensional scanning mirror unit are not intersected；
Reflective mirror, the reflective mirror are arranged in the optical channel；Reflected light on reflective mirror is in the optical channel
Interior transmission；The reflective mirror is greater than 90%, such as 99% to the reflectivity of the rupture of membranes light, which is greater than to the conversion light
Reflectivity, such as 40-60%；And the reflective mirror is to wavelength X3Light reflectivity less than 1%, λ3∈[380nm,λ2∪(λ2,
780nm]。
The working method of the laser microprobe dating instrument of the embodiment of the present invention, the working method the following steps are included:
(A1) the rupture of membranes light that laser issues is incident on wavelength convert component；
(A2) wavelength convert component works, by the wavelength X of the rupture of membranes light1It is transformed into wavelength X2∈[380nm,780nm]；
(A3) conversion light is mapped on embryo after two-dimensional scanning mirror unit, reflective mirror；
Two-dimensional scanning mirror unit is adjusted, so that conversion light is mapped to the designated position of embryo；
The switch that filters is opened, and wavelength X is filtered out1Rupture of membranes light, prevent the indicating positions stage damage embryo；
(A4) working condition for adjusting wavelength convert component, when the rupture of membranes light passes through the wavelength convert component, few portion
Point rupture of membranes light is converted to conversion light, and most of constant the passes through wavelength convert component；Rupture of membranes light and conversion light successively pass through two dimension
The reflection for scanning mirror unit, reflective mirror, is mapped to the designated position of embryo.
Embodiment 2:
Fig. 2 schematically illustrates the structure diagram of the laser microprobe dating instrument of the embodiment of the present invention 2, as shown in Fig. 2, institute
Stating laser microprobe dating instrument includes:
Laser, the wavelength for the rupture of membranes light that the laser issues are λ1, λ1∈ [1400nm, 1500nm],Laser power range is 200mW-600mW.；
Object lens, the object lens include:
Shell, the interior optical channel having along the casing center axis of the shell；The laser is arranged in shell
In vivo, the laser is 0 perpendicular to projected area on the section on the central axis in the optical channel；
Wavelength convert component, such as frequency multiplication component, the wavelength convert component is arranged in the shell, and swashs in described
In the emergent light optical path of light device；The wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2
∈[380nm,780nm]；The transfer efficiency of wavelength convert component is adjustable；
Filter switch, and it is λ that the optical filtering switch filters out wavelength as needed1Rupture of membranes light；
Mirror unit, the mirror unit are arranged in the shell, and the conversion light is reflected into two-dimensional scanning
Mirror unit；Projected area of the mirror unit on the section is 0；The mirror unit includes:
First reflecting mirror, the emergent light are successively reflected by the first reflecting mirror and the second reflecting mirror；
Second reflecting mirror, first reflecting mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel；
Rupture of membranes light and/or conversion light are reflected into reflective mirror by two-dimensional scanning mirror unit, the two-dimensional scanning mirror unit；It is described
The projected area of two-dimensional scanning mirror unit and wavelength convert component on the section be 0, and laser, wavelength convert component,
Optical path and optical channel between mirror unit and two-dimensional scanning mirror unit are not intersected；The two-dimensional scanning mirror unit packet
It includes:
First scanning mirror, first scanning mirror and the second scanning mirror are setting up and down；First scanning mirror, the first reflection
Mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel；
The rotary shaft of second scanning mirror, the first scanning mirror and the second scanning mirror is mutually perpendicular to, to realize two-dimensional scanning function
Energy；
Reflective mirror, the reflective mirror are arranged in the optical channel；Reflected light on reflective mirror is in the optical channel
Interior transmission；The reflective mirror is greater than 90%, such as 99% to the reflectivity of the rupture of membranes light, which is greater than to the conversion light
Reflectivity, such as 40-60%；And the reflective mirror is to wavelength X3Light reflectivity less than 1%, λ3∈[380nm,λ2)∪
(λ2, 780nm].
The working method of the laser microprobe dating instrument of the embodiment of the present invention, the working method the following steps are included:
(A1) the rupture of membranes light that laser issues is incident on wavelength convert component；
(A2) wavelength convert component works, by the wavelength X of the rupture of membranes light1It is transformed into wavelength X2∈[380nm,780nm]；
(A3) conversion light successively by the first reflecting mirror, the second reflecting mirror reflection after be incident on the first scanning mirror,；
It is mapped on embryo after two-dimensional scanning mirror unit, reflective mirror；
Two-dimensional scanning mirror unit is adjusted, so that conversion light is mapped to the designated position of embryo；
The switch that filters is opened, and wavelength X is filtered out1Rupture of membranes light, prevent the indicating positions stage damage embryo；
(A4) working condition for adjusting wavelength convert component, when the rupture of membranes light passes through the wavelength convert component, few portion
Point rupture of membranes light is converted to conversion light, and most of constant the passes through wavelength convert component；Rupture of membranes light and conversion light successively pass through two dimension
The reflection for scanning mirror unit, reflective mirror, is mapped to the designated position of embryo.
Embodiment 3:
According to embodiments of the present invention 2 laser microprobe dating instrument and its application examples of working method.
In the application examples, the wavelength X of the rupture of membranes light of semiconductor laser outgoing1For 1480nm ± 20nm, power 500
±50mW；Wavelength convert component uses frequency multiplication component, the wavelength X of conversion light2For 740nm ± 10nm；First scanning mirror,
One reflecting mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel, between the main shaft of optical path be in perpendicular to shell
Central axis plane on；First scanning mirror is rotated along first rotating shaft, and the second scanning mirror is rotated along the second shaft, and first
Shaft and the second shaft are mutually perpendicular to；Shell cylindrical part, internal void are divided into optical channel, diameter R1；In shell
Between partially have larger section of internal diameter, diameter R2, R2> R1；Above-mentioned laser, wavelength convert component, mirror unit and two dimension
Scanning mirror unit and between optical path be in larger section of internal diameter, and be in R2> R > R1Region in, namely in the light
The projected area learned in the section perpendicular to the central axis of shell in channel is 0.
Claims (10)
1. a kind of laser microprobe dating instrument, the laser microprobe dating instrument includes laser, object lens；It is characterized by: institute
Stating object lens includes:
Shell, the interior optical channel having along the casing center axis of the shell；The laser is arranged in shell,
The wavelength of the rupture of membranes light of outgoing is λ1,The laser is in the optical channel perpendicular to described
The projected area on section on central axis is 0；
Wavelength convert component, the wavelength convert component are arranged in the shell, and are in the emergent light light of the laser
On the road；The wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2∈[380nm,
780nm]；
Filter switch, and the optical filtering switch is λ for preventing wavelength as needed1Rupture of membranes light emission to embryo；
Rupture of membranes light and/or conversion light are reflected into reflective mirror by two-dimensional scanning mirror unit, the two-dimensional scanning mirror unit；The two dimension
Scanning the projected area of mirror unit and wavelength convert component on the section is 0；
Reflective mirror, the reflective mirror are arranged in the optical channel；Reflected light on reflective mirror passes in the optical channel
It is defeated.
2. laser microprobe dating instrument according to claim 1, it is characterised in that: the reflective mirror is anti-to the rupture of membranes light
Rate is penetrated greater than 90%, which is greater than the reflectivity to the conversion light.
3. laser microprobe dating instrument according to claim 2, it is characterised in that: the reflective mirror is to wavelength X3Light it is anti-
Rate is penetrated less than 1%, λ3∈[380nm,λ2)∪(λ2, 780nm].
4. laser microprobe dating instrument according to claim 1, it is characterised in that: the object lens further comprise:
Mirror unit, the mirror unit are arranged in the shell, and the conversion light is reflected into Scan mirror list
Member；Projected area of the mirror unit on the section is 0.
5. laser microprobe dating instrument according to claim 4, it is characterised in that: the mirror unit includes:
First reflecting mirror, the emergent light are successively reflected by the first reflecting mirror and the second reflecting mirror；
Second reflecting mirror, first reflecting mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel.
6. laser microprobe dating instrument according to claim 5, it is characterised in that: the two-dimensional scanning mirror unit includes:
First scanning mirror, first scanning mirror and the second scanning mirror are setting up and down；First reflecting mirror, the second reflecting mirror and
First scanning mirror is arranged along the circumferential direction of the optical channel；
The rotary shaft of second scanning mirror, the first scanning mirror and the second scanning mirror is mutually perpendicular to.
7. laser microprobe dating instrument according to claim 1, it is characterised in that: the wavelength convert component is frequency multiplication portion
Part.
8. laser microprobe dating instrument according to claim 1, it is characterised in that: wavelength X1, λ1∈[1400nm,1500nm]。
9. the working method of -8 any laser microprobe dating instrument according to claim 1, the working method includes following
Step:
(A1) the rupture of membranes light that laser issues is incident on wavelength convert component；
(A2) wavelength convert component works, by the wavelength X of the rupture of membranes light1It is transformed into wavelength X2∈[380nm,780nm]；
(A3) conversion light is mapped on embryo after two-dimensional scanning mirror unit, reflective mirror；
Two-dimensional scanning mirror unit is adjusted, so that conversion light is mapped to the designated position of embryo；Wavelength X1Rupture of membranes light be prevented from being mapped to embryo
On tire；
(A4) working condition of wavelength convert component is adjusted, the rupture of membranes light that laser issues successively passes through the Scan mirror list
The designated position of embryo is mapped to after member and reflective mirror.
10. working method according to claim 9, it is characterised in that: in step (A4), the rupture of membranes light passes through described
When wavelength convert component, small part rupture of membranes light is converted to conversion light, and most of rupture of membranes optical wavelength unchangeably passes through wavelength conversion section
Part；Rupture of membranes light and conversion light are mapped to the designated position of embryo.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810249142X | 2018-03-26 | ||
| CN201810249142 | 2018-03-26 |
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| Publication Number | Publication Date |
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| CN109251857A true CN109251857A (en) | 2019-01-22 |
| CN109251857B CN109251857B (en) | 2023-09-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811297289.2A Active CN109251857B (en) | 2018-03-26 | 2018-11-01 | Laser micro-cutting instrument and working method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111283335A (en) * | 2020-03-24 | 2020-06-16 | 宁波大学 | Laser microdissection device and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201586115U (en) * | 2009-11-23 | 2010-09-22 | 上海康奥医疗科技有限公司 | Laser biological tissue micromachining device |
| US20110317727A1 (en) * | 2009-12-14 | 2011-12-29 | Hiroyuki Furuya | Wavelength conversion light source, optical element and image display device |
| CN106994557A (en) * | 2017-04-20 | 2017-08-01 | 武汉铱科赛科技有限公司 | A kind of dynamic controllable laser-processing system and method for focal position of laser |
| CN209508277U (en) * | 2018-03-26 | 2019-10-18 | 广州市华粤行医疗科技有限公司 | Laser microprobe dating instrument |
-
2018
- 2018-11-01 CN CN201811297289.2A patent/CN109251857B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201586115U (en) * | 2009-11-23 | 2010-09-22 | 上海康奥医疗科技有限公司 | Laser biological tissue micromachining device |
| US20110317727A1 (en) * | 2009-12-14 | 2011-12-29 | Hiroyuki Furuya | Wavelength conversion light source, optical element and image display device |
| CN106994557A (en) * | 2017-04-20 | 2017-08-01 | 武汉铱科赛科技有限公司 | A kind of dynamic controllable laser-processing system and method for focal position of laser |
| CN209508277U (en) * | 2018-03-26 | 2019-10-18 | 广州市华粤行医疗科技有限公司 | Laser microprobe dating instrument |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111283335A (en) * | 2020-03-24 | 2020-06-16 | 宁波大学 | Laser microdissection device and method |
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| CN109251857B (en) | 2023-09-05 |
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