CN109251245A - A method of take beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof - Google Patents
A method of take beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof Download PDFInfo
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- CN109251245A CN109251245A CN201811157951.4A CN201811157951A CN109251245A CN 109251245 A CN109251245 A CN 109251245A CN 201811157951 A CN201811157951 A CN 201811157951A CN 109251245 A CN109251245 A CN 109251245A
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- cow hoof
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- 210000000003 hoof Anatomy 0.000 title claims abstract description 88
- 235000015278 beef Nutrition 0.000 title claims abstract description 70
- 210000002435 tendon Anatomy 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims abstract description 39
- 102000008186 Collagen Human genes 0.000 title claims abstract description 38
- 108010035532 Collagen Proteins 0.000 title claims abstract description 38
- 229920001436 collagen Polymers 0.000 title claims abstract description 37
- 235000015277 pork Nutrition 0.000 title claims abstract description 18
- 239000000284 extract Substances 0.000 claims abstract description 27
- 230000008569 process Effects 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- 239000004519 grease Substances 0.000 claims description 17
- 210000000988 bone and bone Anatomy 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 239000000047 product Substances 0.000 claims description 12
- 230000007062 hydrolysis Effects 0.000 claims description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims description 6
- 238000007605 air drying Methods 0.000 claims description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical group [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052782 aluminium Inorganic materials 0.000 claims description 4
- 230000000903 blocking effect Effects 0.000 claims description 4
- 238000005202 decontamination Methods 0.000 claims description 4
- 230000003588 decontaminative effect Effects 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
- 239000010935 stainless steel Substances 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 abstract description 10
- 108090000790 Enzymes Proteins 0.000 abstract description 10
- 239000000126 substance Substances 0.000 abstract description 6
- 210000003205 muscle Anatomy 0.000 abstract description 5
- 239000013043 chemical agent Substances 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 230000002411 adverse Effects 0.000 abstract description 2
- 238000007781 pre-processing Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 8
- 239000003513 alkali Substances 0.000 description 6
- 238000000605 extraction Methods 0.000 description 5
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- HLLSOEKIMZEGFV-UHFFFAOYSA-N 4-(dibutylsulfamoyl)benzoic acid Chemical compound CCCCN(CCCC)S(=O)(=O)C1=CC=C(C(O)=O)C=C1 HLLSOEKIMZEGFV-UHFFFAOYSA-N 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 239000001828 Gelatine Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- -1 trypsase Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The present invention discloses a kind of method for taking beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof, includes following steps: (1) pre-processing: stripping out beef tendon from cow hoof;(2) it extracts;(3) finished product is dry.The method of the present invention is using the beef tendon stripped out in cow hoof as raw material (being not the big muscle of ox of cow hoof part in succession), it abandons the out-of-date methods that the pretreatment of common chemicals, enzyme extract both at home and abroad and uses pure natural temperature control Hydrolyze method, effectively extract beef tendon collagen, human body adverse conditions may be occurred by avoiding the residue of chemical agent, and it avoids chemical substance that from may destroying the generation of effective component and avoids overall process secondary pollution, it is at low cost, product yield is high, and purity is also high, is worth of widely use.
Description
Technical field
The present invention relates to collagen field technologies, refer in particular to one kind and beef tendon is taken to extract tendons of beef, mutton or pork collagen egg from cow hoof
White method.
Background technique
Collagen is boiomacromolecule, the main component in animal connective tissue, and content is most in the mammalian body
Functional protein more, distribution is most wide, accounts for the 25%~30% of total protein, certain organisms are even as high as 80% or more.
The type of collagen has very much, and relatively conventional has bone collagen, and the bone collagen of the prior art mentions
It takes there are mainly three types of methods, is respectively as follows: 1, acid extraction method, the method is that right using sour (such as citric acid, lactic acid, acetic acid, hydrochloric acid)
The bone collagen in bone is extracted again after ox bone processing.But acid extraction method can destroy the structure of amino acid, extract institute
Acid, which can remain in gelatine albumen, to be caused harm to the human body, and the acid of extraction also has the defect of corrosion equipment;2, alkali
Extraction method, the method is that alkali carries take using certain density alkali to extracting again to the bone collagen in bone after ox bone processing
The common inorganic agent of method has lime, sodium hydroxide, sodium carbonate etc., these inorganic agents can also destroy the amino acid in bone collagen
Structure, and remaining alkali it is edible after have the suspicion for being unfavorable for human body;3, enzyme extraction method, this common enzyme of method have neutral protein
Enzyme, papain, trypsase, pepsin etc., but enzyme preparation has limitation, different protein needs to match not
Same protease, and enzyme preparation is expensive, it is at high cost.With progress of research, there is sour enzyme mixed processing method, alkali enzyme
Mixed processing method etc..And someone uses total mixing method a few days ago, the method is that material is first impregnated 10 points with 5% soda bath
Clock removes grease on surface, and is allowed to expand, then be washed till with clear water without alkali taste, then soaks decalcification in 24 hours with dilute hydrochloric acid, then
It is cleaned with clear water, is then placed in 4% limewash and soaks 10 days, then clean, last hot water extracts.
Market is there is not yet tendons of beef, mutton or pork collagen, tendons of beef, mutton or pork collagen have very high nutrition, health care and treatment function at present,
Tendons of beef, mutton or pork collagen is extracted according to above-mentioned various extracting methods there is material residual, destroy the collagen molecules knot extracted
Structure, the disadvantage that at high cost, product yield is low, purity is not high enough.Therefore, it is necessary to study a kind of methods can be effectively by tendons of beef, mutton or pork glue
Former albumen is extracted from cow hoof.
Summary of the invention
It is taken from cow hoof in view of this, in view of the deficiencies of the prior art, the present invention aims to provide one kind
The method that beef tendon extracts tendons of beef, mutton or pork collagen, can effectively extract tendons of beef, mutton or pork collagen from cow hoof, residual with no material
It stays, molecular structure that collagen will not be destroyed, the feature that at low cost, product yield is high, with high purity.
To achieve the above object, the present invention is using following technical solution:
A method of it takes beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof, includes following steps:
(1) it pre-processes: stripping out beef tendon from cow hoof;
(2) it extracts: including following steps:
(2.1) beef tendon is cut into small pieces and is put into the first container, 1800-2200 milliliters of water of ratio is added in beef tendon per jin
Add water into the first container, the first container is covered, open fire boiled, turn small fire after boiled, controls later by water temperature at 60-100 DEG C
Hydrolysis 25-35 hours is ceased fire, and is removed grease on liquid level with grease trap within every 3 hours in hydrolysis;
(2.2) supernatant of the beef tendon extract hydrolyzed is filtered with grease trap, takes out and cools, have grease solidifying on juice face
It is removed again when knot, then is condensed into the beef tendon extract liquid of content 40%;
(3) finished product is dry: include following steps:
(3.1) it by beef tendon extract liquid upper mold cup, then puts drying shed into and air-dry at least 5 days;
(3.2) break away from moulds cup has been solidified after air-drying and formed cow hoof collagen bulk is put into dry basin 25-35 again
DEG C temperature dry 12 to 15 days and be completely dried to it.
As a preferred embodiment, step (1) pretreatment includes following steps:
(1.1) cow hoof is scrubbed with running water, removes the dirt being stained on cow hoof;
(1.2) cow hoof scrubbed is divided into two incision;
(1.3) it puts the cow hoof separated after scrub into second container, discharges water to flood cow hoof for degree;It opens fire boiled, ten minutes
The crust knocked out on cow hoof is taken out afterwards;
(1.4) it puts the cow hoof for knocking out crust into second container again and adds water to flood cow hoof for degree, open fire boiled 10 minutes, to go
Except retaining dirt;
(1.5) cow hoof after decontamination is taken out, skin, hair and the blocking grease on hoof face is pruned with knife;
(1.6) it puts the cow hoof for eliminating fur into second container again and adds water to flood cow hoof for degree, boiled 30 minutes, then by cow hoof
It takes out and rinses;
(1.7) cow hoof rinsed well is splitted among two bones and strips out beef tendon.
As a preferred embodiment, the second container is aluminum pot or stainless-steel pan.
As a preferred embodiment, beef tendon is cut into small pieces for less than 1cm × 1cm × 0.5 in the step (2.1)
cm。
As a preferred embodiment, the step (3) further comprise have step (3.3) it is absolutely dry after beef tendon collagen
Protein product is made into blocky or powdered.
The present invention has obvious advantages and beneficial effects compared with the existing technology, specifically, by above-mentioned technical proposal
Known to:
The method of the present invention is using the beef tendon stripped out in cow hoof as raw material (being not the big muscle of ox of cow hoof part in succession),
It abandons the out-of-date methods that the pretreatment of common chemicals, enzyme extract both at home and abroad and uses pure natural temperature control Hydrolyze method, effectively extract
Beef tendon collagen out avoids the residue of chemical agent that human body adverse conditions may occur, and avoids chemical substance can
The generation of effective component can be destroyed and avoid overall process secondary pollution, at low cost, product yield is high, and purity is also high, is worth
It promotes the use of.
Detailed description of the invention
Fig. 1 is flow diagram of the invention.
Specific embodiment
The method that present invention discloses a kind of to take beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof, includes following step
It is rapid:
(1) it pre-processes: stripping out beef tendon from cow hoof;Specifically, including following steps:
(1.1) cow hoof is scrubbed with running water, removes the dirt being stained on cow hoof.
(1.2) cow hoof scrubbed is divided into two incision.
(1.3) cow hoof separated after scrub is put into second container, the second container is aluminum pot or stainless-steel pan, is put
Water is to flood cow hoof for degree;It opens fire boiled, takes out the crust knocked out on cow hoof ten minutes later.
(1.4) it puts the cow hoof for knocking out crust into second container again and adds water to flood cow hoof for degree, open fire boiled 10 minutes,
Dirt is retained with removal.
(1.5) cow hoof after decontamination is taken out, skin, hair and the blocking grease on hoof face is pruned with knife.
(1.6) putting second container into again to add water to flood cow hoof by the cow hoof for eliminating fur is to spend, boiled 30 minutes, then will
Cow hoof, which takes out, to be rinsed.
(1.7) cow hoof rinsed well is splitted among two bones and strips out beef tendon.
(2) it extracts: including following steps:
(2.1) beef tendon is cut to the fritter less than the cm of 1cm × 1cm × 0.5 and put into the first container, by cow hoof per jin
Muscle adds 1800-2200 milliliters of water of ratio to add water into the first container, and the first container is covered, and opens fire boiled, turns small fire after boiled,
Later by water temperature control 60-100 DEG C hydrolysis 25-35 hour, truce, hydrolyze when liquid level is oiled with grease trap within every 3 hours
Rouge removes.
(2.2) supernatant of the beef tendon extract hydrolyzed is filtered with grease trap, takes out and cools, have oil on juice face
Rouge is removed again when condensing, then is condensed into the beef tendon extract liquid of content 40%.
(3) finished product is dry: include following steps:
(3.1) it by beef tendon extract liquid upper mold cup, then puts drying shed into and air-dry at least 5 days.
(3.2) break away from moulds cup has been solidified after air-drying and formed cow hoof collagen bulk is put into dry basin use again
25-35 DEG C of temperature, which is dried 12 to 15 days, to be completely dried to it.
(3.3) the beef tendon collagen product after absolutely dry is made into blocky or powdered.
Optimum embodiment:
A method of it takes beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof, includes following steps:
(1) it pre-processes: stripping out beef tendon from cow hoof;Specifically, including following steps:
(1.1) cow hoof is scrubbed with running water, removes the dirt being stained on cow hoof.
(1.2) cow hoof scrubbed is divided into two incision.
(1.3) cow hoof separated after scrub is put into second container, the second container is aluminum pot or stainless-steel pan, is put
Water is to flood cow hoof for degree;It opens fire boiled, takes out the crust knocked out on cow hoof ten minutes later.
(1.4) it puts the cow hoof for knocking out crust into second container again and adds water to flood cow hoof for degree, open fire boiled 10 minutes,
Dirt is retained with removal.
(1.5) cow hoof after decontamination is taken out, skin, hair and the blocking grease on hoof face is pruned with knife.
(1.6) putting second container into again to add water to flood cow hoof by the cow hoof for eliminating fur is to spend, boiled 30 minutes, then will
Cow hoof, which takes out, to be rinsed.
(1.7) cow hoof rinsed well is splitted among two bones and strips out beef tendon.
(2) it extracts: including following steps:
(2.1) beef tendon is cut to the fritter less than the cm of 1cm × 1cm × 0.5 and put into the first container, by cow hoof per jin
Muscle adds 1800-2200 milliliters of water of ratio to add water into the first container, and the first container is covered, and opens fire boiled, turns small fire after boiled,
Later by water temperature control 60-100 DEG C hydrolysis 25-35 hour, truce, hydrolyze when liquid level is oiled with grease trap within every 3 hours
Rouge removes.
(2.2) supernatant of the beef tendon extract hydrolyzed is filtered with grease trap, takes out and cools, have oil on juice face
Rouge is removed again when condensing, then is condensed into the beef tendon extract liquid of content 40%.
(3) finished product is dry: include following steps:
(3.1) it by beef tendon extract liquid upper mold cup, then puts drying shed into and air-dry 5 days.
(3.2) break away from moulds cup has been solidified after air-drying and formed cow hoof collagen bulk is put into dry basin use again
25-35 DEG C of temperature, which is dried 12 to 15 days, to be completely dried to it.
(3.3) the beef tendon collagen product after absolutely dry is made into blocky or powdered.
Design focal point of the invention is: the method for the present invention be using the beef tendon stripped out in cow hoof as raw material (simultaneously
Non- is the big muscle of ox of cow hoof part in succession), it abandons the out-of-date methods that the pretreatment of common chemicals, enzyme extract both at home and abroad and uses pure
Natural temperature control Hydrolyze method, effectively extracts beef tendon collagen, avoids the residue of chemical agent may be unfavorable to human body
It happens, and avoids chemical substance that from may destroying the generation of effective component and avoid overall process secondary pollution, at low cost, product
Yield is high, and purity is also high, is worth of widely use.
The above described is only a preferred embodiment of the present invention, be not intended to limit the scope of the present invention,
Therefore any subtle modifications, equivalent variations and modifications to the above embodiments according to the technical essence of the invention, still
Belong in the range of technical solution of the present invention.
Claims (5)
1. a kind of method for taking beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof, it is characterised in that: include following steps:
(1) it pre-processes: stripping out beef tendon from cow hoof;
(2) it extracts: including following steps:
(2.1) beef tendon is cut into small pieces and is put into the first container, 1800-2200 milliliters of water of ratio is added in beef tendon per jin
Add water into the first container, the first container is covered, open fire boiled, turn small fire after boiled, controls later by water temperature at 60-100 DEG C
Hydrolysis 25-35 hours is ceased fire, and is removed grease on liquid level with grease trap within every 3 hours in hydrolysis;
(2.2) supernatant of the beef tendon extract hydrolyzed is filtered with grease trap, takes out and cools, have grease solidifying on juice face
It is removed again when knot, then is condensed into the beef tendon extract liquid of content 40%;
(3) finished product is dry: include following steps:
(3.1) it by beef tendon extract liquid upper mold cup, then puts drying shed into and air-dry at least 5 days;
(3.2) break away from moulds cup has been solidified after air-drying and formed cow hoof collagen bulk is put into dry basin 25-35 again
DEG C temperature dry 12 to 15 days and be completely dried to it.
2. a kind of method for taking beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof according to claim 1, feature exist
In: step (1) pretreatment includes following steps:
(1.1) cow hoof is scrubbed with running water, removes the dirt being stained on cow hoof;
(1.2) cow hoof scrubbed is divided into two incision;
(1.3) it puts the cow hoof separated after scrub into second container, discharges water to flood cow hoof for degree;It opens fire boiled, ten minutes
The crust knocked out on cow hoof is taken out afterwards;
(1.4) it puts the cow hoof for knocking out crust into second container again and adds water to flood cow hoof for degree, open fire boiled 10 minutes, to go
Except retaining dirt;
(1.5) cow hoof after decontamination is taken out, skin, hair and the blocking grease on hoof face is pruned with knife;
(1.6) it puts the cow hoof for eliminating fur into second container again and adds water to flood cow hoof for degree, boiled 30 minutes, then by cow hoof
It takes out and rinses;
(1.7) cow hoof rinsed well is splitted among two bones and strips out beef tendon.
3. a kind of method for taking beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof according to claim 2, feature exist
In: the second container is aluminum pot or stainless-steel pan.
4. a kind of method for taking beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof according to claim 1, feature exist
In: beef tendon is cut into small pieces for less than the cm of 1cm × 1cm × 0.5 in the step (2.1).
5. a kind of method for taking beef tendon to extract tendons of beef, mutton or pork collagen from cow hoof according to claim 1, feature exist
In: the step (3) further comprise have step (3.3) it is absolutely dry after beef tendon collagen product be made into blocky or powder
Shape.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110403044A (en) * | 2019-07-29 | 2019-11-05 | 青海宏健生物科技集团有限公司 | A kind of collagen soft sweets and preparation method thereof |
CN115989844A (en) * | 2023-03-22 | 2023-04-21 | 成都元能营养食品有限公司 | Collagen composition and preparation method thereof |
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YEHE LIU 等: "Method to extract minimally damaged collagen fibrils from tendon", 《J BIOL METHODS》 * |
杨萌萌 等: "超声波辅助法提取胶原蛋白工艺研究", 《甘肃农业大学学报》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110403044A (en) * | 2019-07-29 | 2019-11-05 | 青海宏健生物科技集团有限公司 | A kind of collagen soft sweets and preparation method thereof |
CN115989844A (en) * | 2023-03-22 | 2023-04-21 | 成都元能营养食品有限公司 | Collagen composition and preparation method thereof |
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