CN109239381A - A kind of micro-fluidic chip and micro fluidic device for biomolecule detection - Google Patents
A kind of micro-fluidic chip and micro fluidic device for biomolecule detection Download PDFInfo
- Publication number
- CN109239381A CN109239381A CN201810870491.3A CN201810870491A CN109239381A CN 109239381 A CN109239381 A CN 109239381A CN 201810870491 A CN201810870491 A CN 201810870491A CN 109239381 A CN109239381 A CN 109239381A
- Authority
- CN
- China
- Prior art keywords
- miniflow
- micro
- connecting element
- cavity
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
Abstract
The invention discloses a kind of micro-fluidic chips and micro fluidic device for biomolecule detection, wherein the micro-fluidic chip includes signal source, first load circuit, first connecting element, two or more miniflow cavities, sensing chip, second connecting element and the second load circuit, wherein, a part of miniflow cavity is input miniflow cavity, for injecting liquid to be detected in the detection zone of sensing chip, another part of miniflow cavity is output miniflow cavity, for emptying the liquid detected, signal source when detection, first load circuit, first connecting element, sensing chip, second connecting element and the second load circuit are sequentially connected in series to form closed circuit, and the electric signal in the output circuit for passing through sensing chip detects the content of biomolecule in liquid to be detected.The present invention is additionally provided with miniflow cavity to convey liquid to be detected to separate electric signal with testing liquid, this is more suitable for real-time measurement and test result is more accurate.
Description
Technical field
The present invention relates to micro-fluidic field more particularly to a kind of micro-fluidic chips for biomolecule detection and micro-fluidic
Device.
Background technique
Biosensor has many advantages, such as low cost, high sensitivity, real-time detection, liquid environment detection, is suitable for
The detection of biomolecule in liquid.It can be to micro-fluidic when chemical/physical/biotic component in liquid is deposited on sensor surface
Electric signal in chip generates interference, by measuring this interference, finally learns the content of macromolecular or particle in a liquid.
However most liquid environments is all conductive, therefore under normal circumstances, the live part of biosensor needs and liquid
Separation.Common solution is that drop to be measured is taken to be detected in the sensitive area of biosensor using micropipette rifle,
But due to the pollution vulnerable to air or due to the evaporation of liquid itself, so that the measurement result of high-precision biosensor is simultaneously
It is unstable.When therefore, in using biosensor measurement liquid, in order to quantitative, quick, stable and inexpensive biology
For sensor application in liquid measure, manufacturing one kind can control testing liquid distribution, versatile, detachable, repeatable benefit
Micro-fluidic chip and device are at key to solve this problem.
Summary of the invention
The present invention is directed to solve at least some of the technical problems in related technologies.For this purpose, of the invention
One purpose be to provide it is a kind of can control testing liquid distribution, versatile, detachable, reusable micro-fluidic chip and
Device.
The technical scheme adopted by the invention is that:
In a first aspect, the present invention provides a kind of micro-fluidic chip for biomolecule detection, the micro-fluidic chip packet
Include signal source, the first load circuit, the first connecting element, two or more miniflow cavities, sensing chip, the second connecting element
And second load circuit, wherein a part of miniflow cavity is input miniflow cavity, for injecting liquid to be detected in sensing
The detection zone of chip, another part of miniflow cavity are output miniflow cavity, for emptying the liquid detected, signal when detection
Source, the first load circuit, the first connecting element, sensing chip, the second connecting element and the second load circuit are sequentially connected in series shape
At closed circuit, and the electric signal in the output circuit for passing through sensing chip detects containing for biomolecule in liquid to be detected
Amount.
Further, wherein sensing chip includes input circuit and output circuit, the first connecting element include the first probe and
Second probe, is connected to the both ends of the input circuit of sensing chip, and the second connecting element includes third probe and the 4th
Probe is connected to the both ends of the output circuit of sensing chip.
Further, the micro-fluidic chip further includes miniflow pump and syringe, wherein miniflow pump passes through syringe when detection
Injection liquid to be detected injects micro liquid to be detected to miniflow cavity, and miniflow pump is injected by syringe after the completion of detection
Air is to miniflow cavity to which emptying has detected liquid.
Second aspect, the present invention provide a kind of micro fluidic device comprising one kind described in first aspect present invention is used for
The micro-fluidic chip of biomolecule detection, and the micro fluidic device includes top plate, middle plate and pedestal, wherein top plate
It is provided with the first through hole being inserted into for the first connecting element and the second connecting element, middle plate is provided with two or more miniflows
Cavity and the second through-hole being inserted into for the first connecting element and the second connecting element are provided with sensing chip, upper layer on pedestal
Plate is additionally provided with the third through-hole for injecting and emptying for liquid to be detected, the quantity and the quantity phase of miniflow cavity of third through-hole
Together, when detection, top plate, middle plate are aligned with pedestal, so that the starting of a part of third through-hole and input miniflow cavity
End alignment is aligned with the another part for injecting liquid to be detected and third through-hole with the end of output miniflow cavity to empty
The liquid of detection, the first connecting element and the second connecting element pass through first through hole, the second through-hole is connected to sensing chip
Both ends.
Further, the position that the middle plate of micro fluidic device corresponds to sensing chip detection zone is provided with hollow reaction
Area.
Further, wherein sensing chip includes input circuit and output circuit, the first connecting element include the first probe and
Second probe, the second connecting element include third probe and the 4th probe, and when detection, the first probe and the second probe connect respectively
It connects at the both ends of the input circuit of sensing chip, third probe and the 4th probe are connected to the output circuit of sensing chip
Both ends.
Further, the micro fluidic device further includes miniflow pump and syringe, the wherein output end and top plate of syringe
The third through-hole of middle alignment input miniflow cavity is tightly connected, so that miniflow pump by syringe injects liquid to be detected when detection
Body injects micro liquid to be detected to miniflow cavity.
Further, the micro fluidic device further includes sealing ring, and the sealing ring is arranged on sensing chip and surrounds biography
The detection zone of sense chip.
Further, the first load circuit and the second load circuit are connected to the both ends of signal source by sub-miniature A connector.
Further, by magnetic attracting device that the top plate, middle plate and pedestal of micro fluidic device is closed together when detection.
The beneficial effects of the present invention are:
The present invention can be quantified by the way that miniflow cavity is arranged with conveying the buffer of liquid to be detected or its certain concentration
Measure the content of liquid to be detected certain ingredient in a certain concentration；
The present invention connects sensing chip by setting connecting element, and it is to be checked to convey to be additionally provided with miniflow cavity
Surveying liquid does not interfere with each other both so that electric signal be separated with testing liquid, this is more suitable for real-time measurement, and
And test result is more accurate；
The position that the middle plate of micro fluidic device of the invention corresponds to sensing chip detection zone is provided with hollow reaction
Liquid to be detected or drop, can be concentrated on the detection zone of sensing chip by area, this is especially suitable for quantitative liquid detecting；
Micro fluidic device of the invention surrounds the detection zone of sensing chip by setting sealing ring, to work as micro-fluidic dress
Further by the closed detection zone for concentrating on sensing chip of liquid to be detected or drop when setting closure, so that liquid to be detected
Or drop will not other regions of infiltration apparatus or by air pollution, which further improves the accuracys of testing result；
Connecting element is set as detachably connected with sensing chip in the present invention, therefore not only convenient for cleaning, can be with
Replacement sensing chip is to repeatedly use；
Micro fluidic device structure of the invention is simple, is easy to use.
Detailed description of the invention
Fig. 1 is according to a kind of structural representation of one embodiment of the micro-fluidic chip for biomolecule detection of the present invention
Figure；
Fig. 2 is according to a kind of structural schematic diagram of one embodiment of micro fluidic device of the present invention；
Fig. 3 is the perspective view of Fig. 2；
Fig. 4 is according to a kind of structural schematic diagram of another embodiment of micro fluidic device of the present invention；
Fig. 5 is according to a kind of structural schematic diagram of another embodiment of micro fluidic device of the present invention；
Fig. 6 is the side view of Fig. 5 embodiment；
Fig. 7 is according to a kind of structural schematic diagram of another embodiment of micro fluidic device of the present invention；
Fig. 8 is according to a kind of structural schematic diagram of another embodiment of micro fluidic device of the present invention.
Specific embodiment
It should be noted that in the absence of conflict, the features in the embodiments and the embodiments of the present application can phase
Mutually combination.
According to first embodiment of the invention, as shown in Figure 1, a kind of micro-fluidic chip for biomolecule detection,
It include signal source, the first load circuit, the first connecting element, two or more miniflow cavity (not shown), sensing chip,
Second connecting element and the second load circuit, wherein a part of miniflow cavity is input miniflow cavity, to be checked for injecting
Liquid is surveyed in the detection zone of sensing chip, another part of miniflow cavity is output miniflow cavity, for emptying the liquid detected
Body, signal source, the first load circuit, the first connecting element, sensing chip, the second connecting element and the second load electricity when detection
Road is sequentially connected in series to form closed circuit, and the electric signal in the output circuit for passing through sensing chip is raw in liquid to be detected to detect
The content of object molecule.For example, the sensing chip can be the bio-sensing chip with certain biological nature, it is also possible to it
He has the sensor of feedback function to electric signal.In another example the electric signal of sensing chip input terminal and output end can be passed through
Frequency variation determine the content of biomolecule in liquid to be detected.
Second embodiment according to the present invention, the basic structure with the above-mentioned one embodiment of the present invention, and
On the basis of above-described embodiment, the sensing chip of the micro-fluidic chip of the present embodiment may include input circuit and output electricity
Road, while the first connecting element may include the first probe and the second probe, be connected to the input circuit of sensing chip
Both ends, the second connecting element may include third probe and the 4th probe, be connected to the output circuit of sensing chip
Both ends.
As the preferred embodiment of the above-mentioned second embodiment of the present invention, wherein the first probe, the second probe, third probe
It can be spring probe with the 4th probe.
As the preferred embodiment of the above-mentioned second embodiment of the present invention, wherein the first load circuit and the second load circuit
The both ends of signal source can be connected to by sub-miniature A connector, so as to can be adapted for frequency range straight for micro-fluidic chip of the invention
It is flow to the application of the microwave regime of 26.5GHz, therefore bandwidth, performance are excellent, highly reliable, the service life is long.
Third embodiment according to the present invention, the basic structure with the above-mentioned one embodiment of the present invention, and
On the basis of above-described embodiment, the micro-fluidic chip of the present embodiment can also include miniflow pump and syringe, wherein when detecting
Miniflow pump injects liquid to be detected to miniflow cavity to inject micro liquid to be detected, miniflow after the completion of detection by syringe
Pump injects air to miniflow cavity to which emptying has detected liquid by syringe.
4th embodiment according to the present invention, as shown in Figures 2 and 3, a kind of micro fluidic device comprising the present invention the
A kind of micro-fluidic chip for biomolecule detection described in one embodiment, and the micro fluidic device can also include upper
Laminate 100, middle plate 200 and pedestal 300, wherein top plate 100 is provided with for the first connecting element and the second connecting element (not
Show) first through hole of insertion, such as 101,102,103 and 104, middle plate 200 is provided with two or more miniflow cavities,
Such as 201 and 202, and for the second through-hole that the first connecting element and the second connecting element are inserted into, such as 203,204,205 and
206, sensing chip (not shown) is provided on pedestal 300.Top plate 100 is additionally provided with to be injected and emptying for liquid to be detected
Third through-hole, such as 105 and 106, the quantity of third through-hole is identical as the quantity of miniflow cavity.When detection, top plate 100,
Middle plate 200 is directed at 300 with pedestal, so that a part of third through-hole is aligned with the starting point of input miniflow cavity to inject
Another part of liquid to be detected and third through-hole is aligned with the end of output miniflow cavity to empty the liquid detected, example
Such as, the position of through-hole 105 corresponds to the starting point of input miniflow cavity 201 and the position of through-hole 106 and corresponds to output miniflow
The end of cavity 202.When detection, the first connecting element and the second connecting element connect respectively across first through hole, the second through-hole
It connects at the both ends of sensing chip, for example, the first connecting element can pass through the through-hole 101 and 102 of top plate and pass through middle layer
The through-hole 203 and 204 of plate and the one end for being connected to sensing chip, the second connecting element can pass through 103 He of through-hole of top plate
It 104 and passes through the through-hole 205 and 206 of middle plate and is connected to the other end of sensing chip.Wherein, although Fig. 2 is merely illustrated
There is the case where through-hole and miniflow cavity of fixed quantity, but micro fluidic device of the invention plate, through-hole on middle plate at the middle and upper levels
Quantity and the quantity of miniflow cavity can specifically be set according to practical application.
As the preferred embodiment of fourth embodiment of the invention, wherein the middle plate 200 of micro fluidic device, which corresponds to, is passed
Hollow reaction zone 207 can be set in the position of sense chip detection zone.Preferably, the size of reaction zone can be equal to or bigger
In the size of sensing chip detection zone.
5th embodiment according to the present invention, the basic structure with the above-mentioned third embodiment of the present invention, and
On the basis of above-described embodiment, a kind of micro fluidic device of the present embodiment is as shown in figure 4, wherein sensing chip 400 includes defeated
Enter circuit 401 and output circuit 402, preferably the first connecting element may include the first probe and the second probe, the second connection
Element includes third probe and the 4th probe (not shown), and when detection, the first probe and the second probe are connected to sensing core
The both ends of the input circuit 402 of piece, third probe and the 4th probe are connected to the two of the output circuit 402 of sensing chip
End.
As the preferred embodiment of above-mentioned 5th embodiment of the present invention, wherein the first probe, the second probe, third probe
It can be spring probe with the 4th probe.
As the preferred embodiment of above-mentioned first to the 5th any embodiment of the present invention, the micro fluidic device can be with
Including miniflow pump and syringe, the third through-hole for inputting miniflow cavity, example wherein are directed in the output end of syringe and top plate
If through-hole 105 is tightly connected, so that miniflow pump is infused by syringe injection liquid to be detected to miniflow cavity when detection
Enter micro liquid to be detected.
According to the sixth embodiment of the present, the basic structure with the above-mentioned third embodiment of the present invention, and
On the basis of above-described embodiment, a kind of micro fluidic device of the present embodiment is as shown in figure 5, wherein the micro fluidic device may be used also
To include sealing ring 500, the sealing ring 500 is arranged on sensing chip 400 and surrounds the detection zone 403 of sensing chip 400.
Preferably, the size of sealing ring 500 is equal or slightly larger than the size of the detection zone 403 of sensing chip 400.Preferably, sealing ring
500 can be the stable silicone gum rubber circle of chemical property.
As the preferred embodiment of the above-mentioned sixth embodiment of the present invention, wherein first through hole is preferably on top plate 100
Be four, such as 101,102,103 and 104, and second through-hole is preferably four on middle plate 200, for example, 203,204,
205 and 206, so that the first probe 601, the second probe 602, third probe 603 and the 4th probe 604 can pass through top plate
100 and middle plate 200 to be connected to the both ends of sensing chip 400.For example, the first probe 601 and the second probe 602 are connected to biography
The both ends of the input circuit of sense chip 400, while the second probe 603 and the 4th probe 604 are connected to the output of sensing chip 400
The both ends of circuit.
As the preferred embodiment of the above-mentioned sixth embodiment of the present invention, as shown in Figure 6 (to put it more simply, part-structure is not
Show), pedestal 300 may be provided with groove 301 and 302, and sensing chip 400 is directly or indirectly fixed on pedestal 300, should
Fixed form can be the mode of buckle, so that sensing chip 400 is so detachable that be fixed on pedestal 300, therefore the present invention
Micro fluidic device it is reusable.
7th embodiment according to the present invention, the basic structure with the above-mentioned third embodiment of the present invention, and
On the basis of above-described embodiment, the micro fluidic device of the present embodiment is (to put it more simply, part-structure is not shown) as shown in Figure 7,
First load circuit and the second load circuit can be connected to the both ends of signal source by two sub-miniature A connectors 701 and 702.
8th embodiment according to the present invention, the basic structure with the above-mentioned third embodiment of the present invention, and
On the basis of above-described embodiment, the micro fluidic device of the present embodiment when detecting can be by magnetic attracting device by micro fluidic device
Top plate, middle plate and pedestal are closed together.
As the preferred embodiment of above-mentioned 8th embodiment of the present invention, the micro fluidic device of the present embodiment can be such as Fig. 8
Shown (to put it more simply, part-structure is not shown), wherein top plate 100 may be provided with magnet placing groove, such as 107,108,109
With 110, while corresponding magnet placing groove is may also set up on pedestal, such as 303,304,305 and 306.In magnet placing groove
It may be provided with magnetic grain.
As the preferred embodiment of above-mentioned 8th embodiment of the present invention, wherein middle plate 200 can be tied with top plate 100
It is combined, it is preferable that can be combined together middle plate 200 and top plate 100 by thermal method.In this way, micro-fluidic
Device has a semi-enclosed reaction zone 207.
As the preferred embodiment of above-mentioned 8th embodiment of the present invention, protrusion has been can be set in insole board 300, such as
307,308,309 and 310, while middle plate 200 can be correspondingly provided with through-hole, such as 208,209,210 and 211.When micro-
When flow control apparatus is closed, the protrusion on pedestal 300 can penetrate the through-hole on middle plate 200 with by middle plate 200 and pedestal 300
Corresponding region alignment, while the magnet in top plate 100 can be attracting entire micro-fluidic to compress with the magnet in pedestal 300
Device.Although Fig. 8 shows the fixed magnet placing groove of quantity and protrusion, the quantity of magnet placing groove of the invention and protrusion
And position can be arranged according to the actual situation and arbitrarily.
As the preferred embodiment of any of the above embodiment of the present invention, wherein micro fluidic device can be by utilizing biofacies
Capacitive is preferable, chemical property is stable acrylic makes.
It is to be illustrated to preferable implementation of the invention, but the invention is not limited to the implementation above
Example, those skilled in the art can also make various equivalent variations on the premise of without prejudice to spirit of the invention or replace
It changes, these equivalent deformations or replacement are all included in the scope defined by the claims of the present application.
Claims (10)
1. a kind of micro-fluidic chip for biomolecule detection, which is characterized in that the micro-fluidic chip includes signal source,
One load circuit, the first connecting element, two or more miniflow cavities, sensing chip, the second connecting element and second are negative
Carry circuit, wherein a part of miniflow cavity is input miniflow cavity, for injecting liquid to be detected in the detection of sensing chip
Area, another part of miniflow cavity are output miniflow cavity, signal source when detection, first negative for emptying the liquid detected
Circuit, the first connecting element, sensing chip, the second connecting element and the second load circuit is carried to be sequentially connected in series to be formed and be closed back
Road, and the electric signal in the output circuit for passing through sensing chip detects the content of biomolecule in liquid to be detected.
2. a kind of micro-fluidic chip for biomolecule detection according to claim 1, which is characterized in that wherein sense
Chip includes input circuit and output circuit, and the first connecting element includes the first probe and the second probe, is connected to biography
The both ends of the input circuit of sense chip, the second connecting element include third probe and the 4th probe, are connected to sensing core
The both ends of the output circuit of piece.
3. a kind of micro-fluidic chip for biomolecule detection according to claim 2, which is characterized in that the miniflow
Control chip further includes miniflow pump and syringe, wherein miniflow pump passes through syringe injection liquid to be detected to miniflow cavity when detection
To inject micro liquid to be detected, miniflow pump injects air to miniflow cavity to empty by syringe after the completion of detection
Detect liquid.
4. a kind of micro fluidic device including micro-fluidic chip described in claim 1, which is characterized in that the micro fluidic device packet
Top plate, middle plate and pedestal are included, wherein top plate is provided with first for the first connecting element and the insertion of the second connecting element
Through-hole, middle plate be provided with two or more miniflow cavity and be inserted into for the first connecting element and the second connecting element the
Two through-holes, are provided with sensing chip on pedestal, top plate be additionally provided with for liquid to be detected inject with the third through-hole that empties, the
The quantity of three through-holes is identical as the quantity of miniflow cavity, and when detection, top plate, middle plate are aligned with pedestal, so that third is logical
The a part in hole with input miniflow cavity starting point be directed at inject another part of liquid to be detected and third through-hole and
To empty the liquid detected, it is logical that the first connecting element and the second connecting element pass through first for the end alignment of output miniflow cavity
Hole, the second through-hole are connected to the both ends of sensing chip.
5. micro fluidic device according to claim 4, which is characterized in that the middle plate of micro fluidic device corresponds to sensing core
The position of piece detection zone is provided with hollow reaction zone.
6. micro fluidic device according to claim 4, which is characterized in that wherein sensing chip includes input circuit and output
Circuit, the first connecting element include the first probe and the second probe, and the second connecting element includes third probe and the 4th probe, when
The first probe and the second probe are connected to the both ends of the input circuit of sensing chip, third probe and the 4th probe when detection
It is connected to the both ends of the output circuit of sensing chip.
7. micro fluidic device according to claim 4, which is characterized in that the micro fluidic device further includes miniflow pump and note
Emitter is wherein directed at the third through-hole sealed connection for inputting miniflow cavity in the output end of syringe and top plate, so that inspection
Miniflow pump injects liquid to be detected to miniflow cavity to inject micro liquid to be detected by syringe when survey.
8. micro fluidic device according to claim 4, which is characterized in that the micro fluidic device further includes sealing ring, institute
It states sealing ring and the detection zone on sensing chip and surrounding sensing chip is set.
9. micro fluidic device according to claim 4, which is characterized in that the first load circuit passes through with the second load circuit
Sub-miniature A connector is connected to the both ends of signal source.
10. micro fluidic device according to claim 4, which is characterized in that pass through magnetic attracting device when detection for micro-fluidic dress
Top plate, middle plate and the pedestal set are closed together.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810870491.3A CN109239381A (en) | 2018-08-02 | 2018-08-02 | A kind of micro-fluidic chip and micro fluidic device for biomolecule detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810870491.3A CN109239381A (en) | 2018-08-02 | 2018-08-02 | A kind of micro-fluidic chip and micro fluidic device for biomolecule detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109239381A true CN109239381A (en) | 2019-01-18 |
Family
ID=65072780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810870491.3A Pending CN109239381A (en) | 2018-08-02 | 2018-08-02 | A kind of micro-fluidic chip and micro fluidic device for biomolecule detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109239381A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111190022A (en) * | 2020-01-07 | 2020-05-22 | 中国科学院半导体研究所 | Biochemical detection system and detection method based on resonant sensor |
| CN113751092A (en) * | 2021-10-19 | 2021-12-07 | 军事科学院***工程研究院卫勤保障技术研究所 | Silicon-based sensor micro-fluidic chip and preparation and packaging method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070238112A1 (en) * | 1999-08-26 | 2007-10-11 | The Trustees Of Princeton University | Microfluidic and nanofluidic electronic devices for detecting changes in capacitance of fluids and methods of using |
| CN101118226A (en) * | 2007-08-06 | 2008-02-06 | 浙江大学 | Multifunctional micro-electrode chip circulation detecting pool device |
| CN103323492A (en) * | 2013-06-03 | 2013-09-25 | 浙江大学 | Rapid assembly micro-flow detection cell device with magnetic separation |
| CN104549591A (en) * | 2015-01-27 | 2015-04-29 | 东南大学 | Universal device for fixing and connecting micro-fluidic chip with electrode |
| CN107699485A (en) * | 2017-11-06 | 2018-02-16 | 东南大学 | Microelectrode fluidic chip and adjustable parameter single cell electroporation device |
| CN207336418U (en) * | 2017-11-08 | 2018-05-08 | 陕西公众智能监测技术有限公司 | A kind of bacteria detecting apparatus based on micro-fluidic two-way dielectrophoresis |
-
2018
- 2018-08-02 CN CN201810870491.3A patent/CN109239381A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070238112A1 (en) * | 1999-08-26 | 2007-10-11 | The Trustees Of Princeton University | Microfluidic and nanofluidic electronic devices for detecting changes in capacitance of fluids and methods of using |
| CN101118226A (en) * | 2007-08-06 | 2008-02-06 | 浙江大学 | Multifunctional micro-electrode chip circulation detecting pool device |
| CN103323492A (en) * | 2013-06-03 | 2013-09-25 | 浙江大学 | Rapid assembly micro-flow detection cell device with magnetic separation |
| CN104549591A (en) * | 2015-01-27 | 2015-04-29 | 东南大学 | Universal device for fixing and connecting micro-fluidic chip with electrode |
| CN107699485A (en) * | 2017-11-06 | 2018-02-16 | 东南大学 | Microelectrode fluidic chip and adjustable parameter single cell electroporation device |
| CN207336418U (en) * | 2017-11-08 | 2018-05-08 | 陕西公众智能监测技术有限公司 | A kind of bacteria detecting apparatus based on micro-fluidic two-way dielectrophoresis |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111190022A (en) * | 2020-01-07 | 2020-05-22 | 中国科学院半导体研究所 | Biochemical detection system and detection method based on resonant sensor |
| CN113751092A (en) * | 2021-10-19 | 2021-12-07 | 军事科学院***工程研究院卫勤保障技术研究所 | Silicon-based sensor micro-fluidic chip and preparation and packaging method thereof |
| CN113751092B (en) * | 2021-10-19 | 2023-02-28 | 军事科学院***工程研究院卫勤保障技术研究所 | Silicon-based sensor micro-fluidic chip and preparation and packaging method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2320053C (en) | Capillary fill device with improved fluid delivery | |
| CN101754812B (en) | Fluidic connectors and microfluidic systems | |
| CN103975242B (en) | Electronics effluent testing arrangement and method | |
| CN109239381A (en) | A kind of micro-fluidic chip and micro fluidic device for biomolecule detection | |
| US20090314066A1 (en) | Sensor device for and a method of sensing particles | |
| Harnsoongnoen et al. | Microwave sensor for nitrate and phosphate concentration sensing | |
| CN108474814A (en) | Continuous, condenser type liquid mobile monitoring in microfluidic device | |
| KR101436150B1 (en) | Biosensor and measuring apparatus therefor | |
| CN101815946A (en) | Disposable analytical microprocessor device | |
| US20100066356A1 (en) | Sensor device comprising means for determining the sample covered area of the sensitive surface | |
| CN105158310A (en) | Microfluidic detection chip based on micropore electrode and application thereof | |
| KR100985475B1 (en) | Sensing apparatus and sensing method using dielectrophoretic impedance | |
| Jao et al. | Electrical characterization of single cell in microfluidic device | |
| CN101354392A (en) | Biosensor, biosensor chip, and biosensor device | |
| JPH0213959Y2 (en) | ||
| CA2009160C (en) | Apparatus for measuring a liquid specimen | |
| US20070158246A1 (en) | Coagulation detection | |
| US11541385B2 (en) | Methods of measuring hematocrit in fluidic channels including conductivity sensor | |
| RU178180U1 (en) | Cartridge for highly specific detection of biomarkers on a quartz resonator | |
| US9623416B2 (en) | Cuvette module having an electrically conductive cuvette carrier | |
| KR102544398B1 (en) | Biofet system | |
| CN111077170A (en) | High-sensitivity microwave microfluidic sensor based on electromagnetic band gap structure | |
| Liu et al. | Single cell impedance analysis and electrical characterization in micro-fluidic device | |
| CN208443458U (en) | A kind of exciting liquid liquid level sensor and liquid container | |
| WO2017167560A1 (en) | Lateral flow test strip comprising moisture-sensitive resistors |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190118 |