CN109234344B - 一种藜麦肽及其制备方法和应用 - Google Patents
一种藜麦肽及其制备方法和应用 Download PDFInfo
- Publication number
- CN109234344B CN109234344B CN201811273826.XA CN201811273826A CN109234344B CN 109234344 B CN109234344 B CN 109234344B CN 201811273826 A CN201811273826 A CN 201811273826A CN 109234344 B CN109234344 B CN 109234344B
- Authority
- CN
- China
- Prior art keywords
- quinoa
- peptide
- enzyme
- protein
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 240000006162 Chenopodium quinoa Species 0.000 title claims abstract description 195
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 118
- 238000002360 preparation method Methods 0.000 title claims abstract description 43
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 66
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 66
- 229940088598 enzyme Drugs 0.000 claims abstract description 52
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 37
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 37
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 37
- 230000007062 hydrolysis Effects 0.000 claims abstract description 29
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 29
- 230000000694 effects Effects 0.000 claims abstract description 28
- 239000000843 powder Substances 0.000 claims abstract description 26
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 235000013305 food Nutrition 0.000 claims abstract description 14
- 238000000227 grinding Methods 0.000 claims abstract description 12
- 239000003208 petroleum Substances 0.000 claims abstract description 12
- 108010007119 flavourzyme Proteins 0.000 claims abstract description 8
- 108091005658 Basic proteases Proteins 0.000 claims abstract description 6
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 6
- 238000005406 washing Methods 0.000 claims abstract description 6
- 230000036772 blood pressure Effects 0.000 claims abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 32
- UUUHXMGGBIUAPW-UHFFFAOYSA-N 1-[1-[2-[[5-amino-2-[[1-[5-(diaminomethylideneamino)-2-[[1-[3-(1h-indol-3-yl)-2-[(5-oxopyrrolidine-2-carbonyl)amino]propanoyl]pyrrolidine-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbon Chemical compound C1CCC(C(=O)N2C(CCC2)C(O)=O)N1C(=O)C(C(C)CC)NC(=O)C(CCC(N)=O)NC(=O)C1CCCN1C(=O)C(CCCN=C(N)N)NC(=O)C1CCCN1C(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C1CCC(=O)N1 UUUHXMGGBIUAPW-UHFFFAOYSA-N 0.000 claims description 27
- 108090000882 Peptidyl-Dipeptidase A Proteins 0.000 claims description 27
- 102000004270 Peptidyl-Dipeptidase A Human genes 0.000 claims description 27
- 239000000758 substrate Substances 0.000 claims description 25
- 102000004190 Enzymes Human genes 0.000 claims description 22
- 108090000790 Enzymes Proteins 0.000 claims description 22
- 238000000605 extraction Methods 0.000 claims description 20
- 229910052757 nitrogen Inorganic materials 0.000 claims description 16
- 229920002472 Starch Polymers 0.000 claims description 13
- 235000019698 starch Nutrition 0.000 claims description 13
- 239000008107 starch Substances 0.000 claims description 13
- 230000002401 inhibitory effect Effects 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 238000000108 ultra-filtration Methods 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 235000013312 flour Nutrition 0.000 claims description 3
- 238000000643 oven drying Methods 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 239000008213 purified water Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 238000001035 drying Methods 0.000 abstract description 8
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 230000004071 biological effect Effects 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 238000002791 soaking Methods 0.000 abstract description 4
- 239000008280 blood Substances 0.000 abstract description 3
- 210000004369 blood Anatomy 0.000 abstract description 3
- 239000000427 antigen Substances 0.000 abstract description 2
- 108091007433 antigens Proteins 0.000 abstract description 2
- 102000036639 antigens Human genes 0.000 abstract description 2
- 238000011161 development Methods 0.000 abstract description 2
- 230000029087 digestion Effects 0.000 abstract description 2
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 230000009466 transformation Effects 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 description 53
- 230000005764 inhibitory process Effects 0.000 description 33
- 239000003392 amylase inhibitor Substances 0.000 description 18
- 238000000034 method Methods 0.000 description 18
- 239000000243 solution Substances 0.000 description 13
- 238000005303 weighing Methods 0.000 description 13
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- FAKRSMQSSFJEIM-RQJHMYQMSA-N captopril Chemical compound SC[C@@H](C)C(=O)N1CCC[C@H]1C(O)=O FAKRSMQSSFJEIM-RQJHMYQMSA-N 0.000 description 6
- 229960000830 captopril Drugs 0.000 description 6
- 238000001816 cooling Methods 0.000 description 6
- 239000003112 inhibitor Substances 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 6
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 5
- 235000015493 Chenopodium quinoa Nutrition 0.000 description 5
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 5
- 229940024606 amino acid Drugs 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 3
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 3
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 235000013922 glutamic acid Nutrition 0.000 description 3
- 239000004220 glutamic acid Substances 0.000 description 3
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 3
- 235000011285 magnesium acetate Nutrition 0.000 description 3
- 239000011654 magnesium acetate Substances 0.000 description 3
- 229940069446 magnesium acetate Drugs 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 239000004474 valine Substances 0.000 description 3
- 101710171801 Alpha-amylase inhibitor Proteins 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000001599 direct drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000413 hydrolysate Substances 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- KEQXNNJHMWSZHK-UHFFFAOYSA-L 1,3,2,4$l^{2}-dioxathiaplumbetane 2,2-dioxide Chemical compound [Pb+2].[O-]S([O-])(=O)=O KEQXNNJHMWSZHK-UHFFFAOYSA-L 0.000 description 1
- 239000005541 ACE inhibitor Substances 0.000 description 1
- 108090000145 Bacillolysin Proteins 0.000 description 1
- 208000003643 Callosities Diseases 0.000 description 1
- 241000871189 Chenopodiaceae Species 0.000 description 1
- 241000222336 Ganoderma Species 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 238000007696 Kjeldahl method Methods 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 108091005507 Neutral proteases Proteins 0.000 description 1
- 102000035092 Neutral proteases Human genes 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229940044094 angiotensin-converting-enzyme inhibitor Drugs 0.000 description 1
- 230000003276 anti-hypertensive effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 238000004380 ashing Methods 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000010304 firing Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 230000007065 protein hydrolysis Effects 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000019643 salty taste Nutrition 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 238000007921 solubility assay Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- -1 superoxide anions Chemical class 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/12—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
- A23J1/125—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses by treatment involving enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Nutrition Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Wood Science & Technology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Biotechnology (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Biophysics (AREA)
- Child & Adolescent Psychology (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
样品名称及编号 | 藜麦肽 | 藜麦蛋白 |
天冬门氨酸(133.1) | 0.1445 | 0.9772 |
苏氨酸(119.1) | 0.0691 | 0.5323 |
丝氨酸(105.1) | 0.0776 | 0.6535 |
谷氨酸(147.1) | 0.2911 | 1.9765 |
甘氨酸(75.1) | 0.0934 | 0.6000 |
丙氨酸(89.1) | 0.0699 | 0.4695 |
缬氨酸(117.2) | 0.1821 | 1.9941 |
蛋氨酸(149.2) | 0.0699 | 0.9611 |
异亮氨酸(131.2) | 0.0647 | 0.3893 |
亮氨酸(131.2) | 0.1055 | 0.6327 |
酪氨酸(181.2) | 0.0500 | 0.5026 |
苯丙氨酸(165.2) | 0.1098 | 1.0000 |
赖氨酸(146.2) | 0.0940 | 0.6047 |
组氨酸(155.2) | 0.0551 | 0.3087 |
精氨酸(174.2) | 0.1402 | 0.7449 |
脯氨酸(115.1) | 0.0744 | 0.4156 |
合计(%) | 1.6900 | 12.8000 |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811273826.XA CN109234344B (zh) | 2018-10-30 | 2018-10-30 | 一种藜麦肽及其制备方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811273826.XA CN109234344B (zh) | 2018-10-30 | 2018-10-30 | 一种藜麦肽及其制备方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109234344A CN109234344A (zh) | 2019-01-18 |
CN109234344B true CN109234344B (zh) | 2020-11-10 |
Family
ID=65079220
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811273826.XA Active CN109234344B (zh) | 2018-10-30 | 2018-10-30 | 一种藜麦肽及其制备方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109234344B (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109770366A (zh) * | 2019-02-25 | 2019-05-21 | 湖北瑞邦生物科技有限公司 | 一种藜麦肽的制备方法 |
CN110241163B (zh) * | 2019-06-25 | 2021-07-27 | 江南大学 | 一种碱提-膜法提取藜麦多肽的方法 |
CN110951809A (zh) * | 2019-09-04 | 2020-04-03 | 内蒙古立泰锐晟智能科技有限公司 | 一种红藜麦多肽粉的制作工艺 |
CN110786519A (zh) * | 2019-10-15 | 2020-02-14 | 广东羲准生物科技有限公司 | 藜麦肽和大豆肽的混合物 |
CN111000249A (zh) * | 2019-11-29 | 2020-04-14 | 南京迪维奥医药科技有限公司 | 一种减肥降糖复方植物多肽口服液及其制备方法 |
CN111197066B (zh) * | 2020-01-10 | 2023-05-16 | 成都大学 | 具有胆酸盐吸附作用的藜麦活性肽的酶解方法 |
CN111893157A (zh) * | 2020-08-17 | 2020-11-06 | 西北农林科技大学 | 一种降血糖藜麦肽及其制备方法和应用 |
CN113337563B (zh) * | 2021-06-01 | 2022-09-30 | 科丽思化妆品(上海)有限公司 | 一种具有美白、抗氧化活性的藜麦肽及其制备方法和应用 |
CN115286696B (zh) * | 2022-06-27 | 2024-02-27 | 上海理工大学 | 一种藜麦肽纳米载体及其制备方法和应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105076665A (zh) * | 2015-07-20 | 2015-11-25 | 聊城大学 | 一种藜麦蛋白粉的制备工艺 |
WO2016105215A1 (es) * | 2014-12-22 | 2016-06-30 | Alicorp S.A.A. | Procedimiento para obtener un instantáneo de quinua |
CN107319302A (zh) * | 2017-08-02 | 2017-11-07 | 李志军 | 一种以糙米和藜麦为主料的幼儿冲调粉及其制备方法 |
CN108041442A (zh) * | 2010-12-08 | 2018-05-18 | 雀巢产品技术援助有限公司 | 含水解的全谷粒的填充组合物 |
-
2018
- 2018-10-30 CN CN201811273826.XA patent/CN109234344B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108041442A (zh) * | 2010-12-08 | 2018-05-18 | 雀巢产品技术援助有限公司 | 含水解的全谷粒的填充组合物 |
WO2016105215A1 (es) * | 2014-12-22 | 2016-06-30 | Alicorp S.A.A. | Procedimiento para obtener un instantáneo de quinua |
CN105076665A (zh) * | 2015-07-20 | 2015-11-25 | 聊城大学 | 一种藜麦蛋白粉的制备工艺 |
CN107319302A (zh) * | 2017-08-02 | 2017-11-07 | 李志军 | 一种以糙米和藜麦为主料的幼儿冲调粉及其制备方法 |
Non-Patent Citations (2)
Title |
---|
"Impact of protease and amylase treatment on proteins and the product quality of a quinoa-based milk substitute";Stephanie Jeske et al.;《The Royal Society of Chemistry》;20180612(第9期);参见摘要,第3501页左栏第2段-第3505页左栏第1段 * |
"Release of dipeptidyl peptidase IV, a-amylase and a-glucosidase inhibitory peptides from quinoa (Chenopodium quinoa Willd.) during in vitro simulated gastrointestinal digestion";Rubén Vilcacundo et al.;《 Journal of Functional Foods》;20170621;第35卷;参见摘要,第532页左栏第3段-第538页左栏第3段 * |
Also Published As
Publication number | Publication date |
---|---|
CN109234344A (zh) | 2019-01-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109234344B (zh) | 一种藜麦肽及其制备方法和应用 | |
Li et al. | Optimization of ACE inhibitory peptides from black soybean by microwave-assisted enzymatic method and study on its stability | |
CN107141336B (zh) | 具有dpp-iv抑制活性的牦牛骨蛋白肽及制备方法 | |
Rui et al. | Angiotensin I-converting enzyme inhibitory properties of Phaseolus vulgaris bean hydrolysates: Effects of different thermal and enzymatic digestion treatments | |
Arise et al. | In vitro antioxidant and α-amylase inhibitory properties of watermelon seed protein hydrolysates. | |
CN101824455B (zh) | 一种大豆蛋白低聚肽及其制备方法和用途 | |
AU666531B2 (en) | Processes for the preparation of amylase inhibitor | |
CN111019989B (zh) | 一种豌豆低聚肽粉及其制备方法 | |
AO et al. | Moringa oleifera seed protein hydrolysates: kinetics of α-amylase inhibition and antioxidant potentials | |
CN108893515B (zh) | 高f值寡肽及其制备方法 | |
CN112961893A (zh) | 罗非鱼皮胶原蛋白抗氧化肽及制备方法和在制备化妆品或细胞氧化损伤保护的药物中的应用 | |
JP2009029772A (ja) | 酵素処理ローヤルゼリー、並びにそれを含有する抗酸化剤、保湿剤、皮膚繊維芽細胞の増殖促進剤、血圧降下剤、疲労回復剤、及びカルシウム吸収促進剤 | |
CN112680491A (zh) | 一种制备玉米黄粉高f值寡肽的方法 | |
CN112795611B (zh) | 一种不可溶性蛋白制备核桃蛋白多肽的方法 | |
CN107723328B (zh) | 一种瓜尔豆活性肽及其制备方法 | |
KR101647558B1 (ko) | 참치심장으로부터 분리한 항산화 펩타이드의 제조방법 | |
CN101366442A (zh) | 一种羊血红蛋白降血压肽及制备方法 | |
CN114989250B (zh) | 一种来源于海水珍珠的血管紧张素转化酶抑制多肽及其应用 | |
Sun et al. | Optimization of enzymatic hydrolysis conditions for preparation of gingko peptides from ginkgo nuts | |
CN114213512B (zh) | 增强藻胆蛋白光热稳定性的组合物及其制备方法与应用 | |
CN113999884B (zh) | 一种甲鱼生物活性肽的制备方法 | |
CN113087773B (zh) | 一种具有降血糖和抗氧化功能的牦牛骨肽及其制备方法 | |
WO2022032803A1 (zh) | 燕窝提取方法、提取物及其产品 | |
CN109554428B (zh) | 一种发酵法高效制备低苦味抗氧化玉米低聚肽的方法 | |
AU2021106749A4 (en) | Whey protein peptide with muscle-building effect and preparation method thereof, and compound protein powder |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information |
Inventor after: Yu Xiaoxiao Inventor after: Su Yuchun Inventor after: Li Yanli Inventor after: Chen Guang Inventor after: Sun Yang Inventor after: Wang Gang Inventor after: Chen Huan Inventor after: Su Yingjie Inventor after: Han Chong Inventor after: Tang Shanshan Inventor after: Wu Zhuofu Inventor before: Chen Guang Inventor before: Su Yuchun Inventor before: Li Yanli Inventor before: Yu Xiaoxiao Inventor before: Han Chong Inventor before: Sun Yang Inventor before: Wang Gang Inventor before: Chen Huan Inventor before: Su Yingjie Inventor before: Tang Shanshan Inventor before: Wu Zhuofu |
|
CB03 | Change of inventor or designer information | ||
GR01 | Patent grant | ||
GR01 | Patent grant |