CN109225363B - Digital control platform based on surface tension - Google Patents
Digital control platform based on surface tension Download PDFInfo
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- CN109225363B CN109225363B CN201811120622.2A CN201811120622A CN109225363B CN 109225363 B CN109225363 B CN 109225363B CN 201811120622 A CN201811120622 A CN 201811120622A CN 109225363 B CN109225363 B CN 109225363B
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- 239000007788 liquid Substances 0.000 claims abstract description 172
- 238000002347 injection Methods 0.000 claims abstract description 63
- 239000007924 injection Substances 0.000 claims abstract description 63
- 238000000605 extraction Methods 0.000 claims abstract description 60
- 239000002245 particle Substances 0.000 claims abstract description 24
- 239000000314 lubricant Substances 0.000 claims description 25
- 239000004094 surface-active agent Substances 0.000 claims description 13
- 230000005012 migration Effects 0.000 claims description 12
- 238000013508 migration Methods 0.000 claims description 12
- 230000000149 penetrating effect Effects 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 abstract description 10
- 238000004458 analytical method Methods 0.000 abstract description 6
- 238000002156 mixing Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 230000000007 visual effect Effects 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004720 dielectrophoresis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
- B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention relates to a digital control platform based on surface tension, and belongs to the technical field of microfluidics. The digital control platform based on the surface tension comprises a rectangular control platform, a liquid drop injection and extraction pipeline, a groove, a liquid supply pipeline, a liquid supply system main valve, a control valve, a circular through hole and a support. The invention does not affect the PH value, surface charge, temperature, viscosity and other parameters of the controlled particles and liquid drops, and the activity of the specimen, so the invention can be used in the basic operations of sample preparation, mixing, reaction and transportation in biological, chemical and medical analysis processes. The invention can modify the size of the rectangular control platform according to actual needs, and can finish the preparation and pretreatment operations of hundreds of samples in a few minutes or even shorter.
Description
Technical Field
The invention relates to a digital control platform based on surface tension, and belongs to the technical field of microfluidics.
Background
The open microfluidic platform has important practical significance in the fields of biomedicine, chemistry, development of new drugs and the like, and the carrier-liquid drops serving as drugs or reactants need to be transported from one position to another, and the traditional method is to adopt a pipettor for manual operation. Such a method requires a lot of manpower and time and is error-prone to operate, while the small volume of droplets is kept unchanged and moved a small distance, which becomes impractical. For example, bacterial dry stained paper (prepared by absorbing a stain containing a certain bacteria on a filter paper sheet and drying, water is added for infiltration during use to precipitate bacterial cells in the stained smear, and observation under a microscope) is produced, and operations such as specimen smear, natural drying, flame fixation and the like are required to be manually performed, so that the operation time is long, the specimen concentration is inconsistent, and the stain consumption is large.
The microfluidic chip is a hotspot field of the development of the current micro total analysis system, and is a basic operation unit which takes a micro-scale chip as an operation platform and can automatically complete sample preparation, reaction, separation and the like in biological, chemical and medical analysis processes. The microfluidic chip has the advantages of controllable fluid flow, less reagent and sample consumption, high analysis speed, small volume and portability. The microfluidic chip adopts electrowetting, dielectrophoresis, thermal capillary method and the like to realize the manipulation and transportation of liquid drops or cells, but the particles or cells are influenced by electric field force and temperature field to cause the increase of dead volume and analysis errors. In recent years, a plurality of researchers at home and abroad push the liquid drop to migrate by producing a surface tension gradient on a control panel, and a layer of lubricant is paved on the control panel to reduce driving force, but mass consumption is generated due to heating evaporation in the movement process of the liquid drop.
The surface tension gradient is used as power to move the tiny objects (solid or liquid drops), so that the influence of electric field force and direct contact with the controlled object are avoided. The factors influencing the surface tension are the concentration of the surfactant and the concentration of the solution per se, and the influence of a temperature field on the controlled object can be avoided by adopting a method for changing the concentration of the surfactant or the concentration of the solution per se to generate a surface tension gradient.
Disclosure of Invention
Aiming at the problems and the defects existing in the prior art, the invention provides a digital control platform based on surface tension. The surface tension-based digital control platform is a precise control platform which is not in direct contact and is efficient in controlling solid particles or liquid drops. The invention is realized by the following technical scheme.
The digital control platform based on the surface tension comprises a rectangular control platform 1, a liquid drop injection and extraction pipeline 2, a groove 3, a liquid supply pipeline 4, a liquid supply system main valve 5, a control valve 6, a circular through hole 7 and a support 8;
the rectangular control platform 1 is a concave plate platform with four side plates on the top surface, and the four corners of the bottom surface of the rectangular control platform 1 are uniformly provided with supports 8;
the four side plates of the rectangular control platform 1 are respectively and uniformly provided with a plurality of liquid drop injection and extraction pipelines 2 penetrating through the side plates, the liquid drop injection and extraction pipelines 2 on each side plate are equally spaced, symmetrically distributed, the center line of the liquid drop injection and extraction pipeline 2 is vertical to the surface of the side plate of the rectangular control platform 1, and the outer side of the liquid drop injection and extraction pipeline 2 can be connected with a liquid drop injection device or an extraction device;
the bottom surface of the rectangular control platform 1 is provided with a plurality of evenly distributed grooves 3, the bottom center of each groove 3 is provided with a corresponding circular through hole 7, and the grooves 3 are communicated with the liquid supply pipeline 4 through the circular through holes 7; the liquid supply pipeline 4 consists of a main pipeline and a plurality of branches, one ends of the branches are respectively communicated with the circular through holes 7 through the control valve 6 and then are connected with the grooves 3, the other ends of the branches are connected to the main pipeline of the liquid supply pipeline 4, and the main pipeline of the liquid supply pipeline 4 is provided with a liquid supply system main valve 5;
when the digital control platform based on surface tension is used, a layer of liquid which is not compatible with controlled liquid drops or particles is paved in the rectangular control platform 1 to serve as a surface lubricant, the surface lubricant liquid level is immersed in the groove 3, and a surfactant capable of changing the surface tension of the surface lubricant liquid is introduced into the liquid supply pipeline 4.
The control valve 6 is a wireless control valve, the wireless control valve is connected with a valve control device, the valve control device is connected with a terminal server through a wireless communication network, the valve control device is used for receiving a command of the terminal server, sending back data, inquiring a valve state and controlling a valve function, and the terminal server is used for calculating the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point. The valve control device uses a single chip microcomputer as a main control chip, and can realize the functions of receiving a terminal server instruction, sending back data, inquiring valve state, controlling a valve and the like (the wireless remote valve control device described by CN201410307887.9 can be adopted), and the terminal server adopts software environments such as Visual basic, visual C++, labview and the like to calculate the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point.
The horizontal distance between the liquid drop injection and extraction pipeline 2 on each side plate of the rectangular control platform 1 is at least 6-10 times of the side length of the groove 3, the height of the liquid drop injection and extraction pipeline is greater than two thirds of the height of the rectangular control platform 1, and the inner side length of the instillation injection and extraction pipeline 2 penetrating through the side plate at least reaches the second row of grooves 3 of the rectangular control platform 1.
The section of the liquid drop injection and extraction pipeline 2 is circular, and the diameter is 50-700 mu m.
The groove 3 should have a side length smaller than one half of the diameter of the droplet or particle to be controlled.
The diameter of the circular through hole 7 is smaller than the side length of the groove 3, and the diameter is 0.1-1 mm.
When the controlled carrier is a particle, the liquid drop injection and extraction pipeline 2 can not be arranged.
The diameter of the particles or liquid drops to be controlled on the rectangular control platform 1 is 2-6 mm.
The working principle of the digital control platform based on the surface tension is as follows:
the control object of the digital control platform based on the surface tension is particles or liquid drops, a surface lubricant which is not compatible with the controlled particles or liquid drops is paved in the rectangular control platform 1, the liquid level of the surface lubricant is slightly higher than the groove 3, the control of the liquid drops is taken as an illustration of the operation process of the control platform by using the surface tension gradient generated by injecting the surfactant, and the principle of changing the concentration of the solution to control the particles or liquid drops is similar. Firstly, pumping controlled liquid drops from a liquid drop injection and extraction pipeline 2 to a surface lubricant in a rectangular control platform 1 by using an injection pump or a micro-flow pump, determining coordinates of a groove 3 corresponding to the position of the liquid drops according to serial numbers of the selected liquid drop injection and extraction pipeline 2 as shown in fig. 3 (a) -3 (b), setting end point coordinates of liquid film migration on a terminal server, and controlling opening and closing time of a valve 6 under the groove 3 corresponding to the position of the liquid drops by a control system through calculation and selection of an optimal direction and a shortest path reaching the end point; then the terminal server sends an instruction to the valve control device through a wireless communication network, the valve control device opens the corresponding control valve 6, and surfactant with different concentrations is released in the groove 3 (the surfactant refers to a substance which can cause the interface state of a solution system to change obviously by adding a small amount, and the molecular structure of the surfactant has amphipathy, wherein one end is hydrophilic and the other end is hydrophobic), as shown in fig. 3 (c); the surfactant is rapidly adsorbed on the gas-liquid interface of the surface lubricant in a row after reaching a certain concentration (the property of the surface lubricant determines the end of the active agent molecule adsorbed on the gas-liquid interface), the surface tension of the position is reduced, the surface tension of the position along the end point direction is unchanged, and the liquid drop is subjected to the action of the surface tension gradient to migrate towards the end point coordinate at a speed V, as shown in fig. 3 (d). In the whole process, the movement direction and speed of the liquid drops are digitally controlled by a control system on the terminal service server.
The beneficial effects of the invention are as follows:
1. the invention can accurately regulate and control the two-dimensional movement of the liquid drop under the conditions of no contact and no damage to the controlled particles and the liquid drop, and can realize digital control.
2. For relieving the burden of a user, a wireless digital control system suitable for various basic operations can be adopted to enable non-professional persons to control the platform, so that the platform is flexible to control and wide in application range.
3. The invention does not affect the PH value, surface charge, temperature, viscosity and other parameters of the controlled particles and liquid drops, and the activity of the specimen, so the invention can be used in the basic operations of sample preparation, mixing, reaction and transportation in biological, chemical and medical analysis processes.
4. The invention can modify the size of the rectangular control platform according to actual needs, and can finish the preparation and pretreatment operations of hundreds of samples in a few minutes or even shorter.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic partial cross-sectional view of the present invention;
FIG. 3 is a schematic diagram of the principles of the present invention;
FIG. 4 is a schematic illustration of droplet mixing for example 1 of the present invention;
FIG. 5 is a schematic illustration of a specimen smear of a bacterial dried stained paper sheet of example 2 of the present invention.
In the figure: the device comprises a 1-rectangular control platform, a 2-liquid drop injection and extraction pipeline, a 3-groove, a 4-liquid supply pipeline, a 5-liquid supply system main valve, a 6-control valve, a 7-circular through hole and an 8-support.
Detailed Description
The invention will be further described with reference to the drawings and detailed description.
Example 1
As shown in fig. 1 to 2, the surface tension-based digital control platform comprises a rectangular control platform 1, a liquid drop injection and extraction pipeline 2, a groove 3, a liquid supply pipeline 4, a liquid supply system total valve 5, a control valve 6, a circular through hole 7 and a support 8;
the rectangular control platform 1 is a concave plate platform with four side plates on the top surface, and the four corners of the bottom surface of the rectangular control platform 1 are uniformly provided with supports 8;
the four side plates of the rectangular control platform 1 are respectively and uniformly provided with a plurality of liquid drop injection and extraction pipelines 2 penetrating through the side plates, the liquid drop injection and extraction pipelines 2 on each side plate are equally spaced, symmetrically distributed, the center line of the liquid drop injection and extraction pipeline 2 is vertical to the surface of the side plate of the rectangular control platform 1, and the outer side of the liquid drop injection and extraction pipeline 2 can be connected with a liquid drop injection device or an extraction device;
the bottom surface of the rectangular control platform 1 is provided with a plurality of evenly distributed grooves 3, the bottom center of each groove 3 is provided with a corresponding circular through hole 7, and the grooves 3 are communicated with the liquid supply pipeline 4 through the circular through holes 7; the liquid supply pipeline 4 consists of a main pipeline and a plurality of branches, one ends of the branches are respectively communicated with the circular through holes 7 through the control valve 6 and then are connected with the grooves 3, the other ends of the branches are connected to the main pipeline of the liquid supply pipeline 4, and the main pipeline of the liquid supply pipeline 4 is provided with a liquid supply system main valve 5;
when the digital control platform based on surface tension is used, a layer of liquid which is not compatible with controlled liquid drops or particles is paved in the rectangular control platform 1 to serve as a surface lubricant, the surface lubricant liquid level is immersed in the groove 3, and a surfactant capable of changing the surface tension of the surface lubricant liquid is introduced into the liquid supply pipeline 4.
The control valve 6 is a wireless control valve, the wireless control valve is connected with a valve control device, the valve control device is connected with a terminal server through a wireless communication network, the valve control device is used for receiving a command of the terminal server, sending back data, inquiring a valve state and controlling a valve function, and the terminal server is used for calculating the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point. The valve control device takes an 89C51 singlechip as a main control chip, can realize the functions of receiving a terminal server instruction, sending back data, inquiring valve state, controlling a valve and the like (the wireless remote valve control device can be adopted in CN 201410307887.9), and the terminal server adopts software environments such as Visual basic, visual C++, labview and the like to calculate the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point.
The rectangular control platform 1 is made of a transparent silicon wafer, two liquid drop injection and extraction pipelines 2 are arranged on one pair of opposite side plates of the rectangular control platform 1, four liquid drop injection and extraction pipelines 2 are arranged on the other pair of opposite side plates, the horizontal distance between the liquid drop injection and extraction pipelines 2 is 6 times of the side length of the groove 3, the height is greater than two thirds of the height of the rectangular control platform 1, and the inner side length of the instillation injection and extraction pipeline 2 penetrating through the side plates at least reaches the second row of grooves 3 (the inner side length is 4 mm) of the rectangular control platform 1; the section of the liquid drop injection and extraction pipeline 2 is circular, and the diameter is 200 mu m; the side length of the groove 3 is smaller than one half of the diameter of the controlled liquid drop or particle; the diameter of the circular through hole 7 is smaller than the side length of the groove 3, and the diameter is 1mm.
The surface tension-based digital control platform in this embodiment is used for controlling droplet mixing, a schematic diagram of droplet mixing is shown in fig. 4, first, controlled droplets a and B are pumped onto a surface lubricant in a rectangular control platform 1 from droplet injection and extraction pipelines 2 with serial numbers of 1 and 4 respectively by using an injection pump, wherein the droplets a and B are different reagents to be mixed, an initial coordinate and an end point coordinate of the droplet a are set on a working interface of a control system, and the control system selects an optimal direction and a shortest path reaching the end point and opening and closing time of a wireless control valve 6 by calculation; drop a is then subjected to a surface tension gradient to migrate at a velocity V toward the endpoint coordinate, mixing with drop B, as shown in fig. 4 (B). Finally, the droplet mixture is pumped out into a collection tube through a droplet injection and pump-out line No. 4 (c), as shown in fig. 4 (c).
Example 2
As shown in fig. 1 to 2, the surface tension-based digital control platform comprises a rectangular control platform 1, a liquid drop injection and extraction pipeline 2, a groove 3, a liquid supply pipeline 4, a liquid supply system total valve 5, a control valve 6, a circular through hole 7 and a support 8;
the rectangular control platform 1 is a concave plate platform with four side plates on the top surface, and the four corners of the bottom surface of the rectangular control platform 1 are uniformly provided with supports 8;
the four side plates of the rectangular control platform 1 are respectively and uniformly provided with a plurality of liquid drop injection and extraction pipelines 2 penetrating through the side plates, the liquid drop injection and extraction pipelines 2 on each side plate are equally spaced, symmetrically distributed, the center line of the liquid drop injection and extraction pipeline 2 is vertical to the surface of the side plate of the rectangular control platform 1, and the outer side of the liquid drop injection and extraction pipeline 2 can be connected with a liquid drop injection device or an extraction device;
the bottom surface of the rectangular control platform 1 is provided with a plurality of evenly distributed grooves 3, the bottom center of each groove 3 is provided with a corresponding circular through hole 7, and the grooves 3 are communicated with the liquid supply pipeline 4 through the circular through holes 7; the liquid supply pipeline 4 consists of a main pipeline and a plurality of branches, one ends of the branches are respectively communicated with the circular through holes 7 through the control valve 6 and then are connected with the grooves 3, the other ends of the branches are connected to the main pipeline of the liquid supply pipeline 4, and the main pipeline of the liquid supply pipeline 4 is provided with a liquid supply system main valve 5;
when the digital control platform based on surface tension is used, a layer of liquid which is not compatible with controlled liquid drops or particles is paved in the rectangular control platform 1 to serve as a surface lubricant, the surface lubricant liquid level is immersed in the groove 3, and a surfactant capable of changing the surface tension of the surface lubricant liquid is introduced into the liquid supply pipeline 4.
The control valve 6 is a wireless control valve, the wireless control valve is connected with a valve control device, the valve control device is connected with a terminal server through a wireless communication network, the valve control device is used for receiving a command of the terminal server, sending back data, inquiring a valve state and controlling a valve function, and the terminal server is used for calculating the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point. The valve control device takes an 89C51 singlechip as a main control chip, can realize the functions of receiving a terminal server instruction, sending back data, inquiring valve state, controlling a valve and the like (the wireless remote valve control device can be adopted in CN 201410307887.9), and the terminal server adopts software environments such as Visual basic, visual C++, labview and the like to calculate the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point.
The rectangular control platform 1 is made of a transparent silicon wafer, two liquid drop injection and extraction pipelines 2 are arranged on one pair of opposite side plates of the rectangular control platform 1, four liquid drop injection and extraction pipelines 2 are arranged on the other pair of opposite side plates, the horizontal distance between the liquid drop injection and extraction pipelines 2 is 10 times of the side length of the groove 3, the height is greater than two thirds of the height of the rectangular control platform 1, and the inner side length of the instillation injection and extraction pipeline 2 penetrating through the side plates at least reaches the second row of grooves 3 (the inner side length is 4 mm) of the rectangular control platform 1; the section of the liquid drop injection and extraction pipeline 2 is circular, and the diameter is 700 mu m; the side length of the groove 3 is smaller than one half of the diameter of the controlled liquid drop or particle; the diameter of the circular through hole 7 is smaller than the side length of the groove 3, and the diameter is 0.1mm.
The digitized control platform based on surface tension in the embodiment is used for preparing a specimen smear of bacterial dry stained paper sheets, the controlled liquid drop is a stained liquid containing a certain bacterial concentration, a qualitative filter paper sheet is hung at a certain position above the rectangular control platform 1, the stained liquid is transported to the lower part of the filter paper sheet through a control system, and the filter paper sheet absorbs the stained liquid, so that the specimen smear is completed, as shown in fig. 5.
Example 3
As shown in fig. 1 to 2, the surface tension-based digital control platform comprises a rectangular control platform 1, a liquid drop injection and extraction pipeline 2, a groove 3, a liquid supply pipeline 4, a liquid supply system total valve 5, a control valve 6, a circular through hole 7 and a support 8;
the rectangular control platform 1 is a concave plate platform with four side plates on the top surface, and the four corners of the bottom surface of the rectangular control platform 1 are uniformly provided with supports 8;
the four side plates of the rectangular control platform 1 are respectively and uniformly provided with a plurality of liquid drop injection and extraction pipelines 2 penetrating through the side plates, the liquid drop injection and extraction pipelines 2 on each side plate are equally spaced, symmetrically distributed, the center line of the liquid drop injection and extraction pipeline 2 is vertical to the surface of the side plate of the rectangular control platform 1, and the outer side of the liquid drop injection and extraction pipeline 2 can be connected with a liquid drop injection device or an extraction device;
the bottom surface of the rectangular control platform 1 is provided with a plurality of evenly distributed grooves 3, the bottom center of each groove 3 is provided with a corresponding circular through hole 7, and the grooves 3 are communicated with the liquid supply pipeline 4 through the circular through holes 7; the liquid supply pipeline 4 consists of a main pipeline and a plurality of branches, one ends of the branches are respectively communicated with the circular through holes 7 through the control valve 6 and then are connected with the grooves 3, the other ends of the branches are connected to the main pipeline of the liquid supply pipeline 4, and the main pipeline of the liquid supply pipeline 4 is provided with a liquid supply system main valve 5;
when the digital control platform based on surface tension is used, a layer of liquid which is not compatible with controlled liquid drops or particles is paved in the rectangular control platform 1 to serve as a surface lubricant, the surface lubricant liquid level is immersed in the groove 3, and a surfactant capable of changing the surface tension of the surface lubricant liquid is introduced into the liquid supply pipeline 4.
The control valve 6 is a wireless control valve, the wireless control valve is connected with a valve control device, the valve control device is connected with a terminal server through a wireless communication network, the valve control device is used for receiving a command of the terminal server, sending back data, inquiring a valve state and controlling a valve function, and the terminal server is used for calculating the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point. The valve control device takes an 89C51 singlechip as a main control chip, can realize the functions of receiving a terminal server instruction, sending back data, inquiring valve state, controlling a valve and the like (the wireless remote valve control device described by CN201410307887.9 can be adopted), and the terminal server adopts software environments such as Visual basic, visual C++, labview and the like to calculate the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point.
The rectangular control platform 1 is made of a transparent silicon wafer, two liquid drop injection and extraction pipelines 2 are arranged on one pair of opposite side plates of the rectangular control platform 1, four liquid drop injection and extraction pipelines 2 are arranged on the other pair of opposite side plates, the horizontal distance between the liquid drop injection and extraction pipelines 2 is 8 times of the side length of the groove 3, the height is greater than two thirds of the height of the rectangular control platform 1, and the inner side length of the instillation injection and extraction pipeline 2 penetrating through the side plates at least reaches the second row of grooves 3 (the inner side length is 4 mm) of the rectangular control platform 1; the section of the liquid drop injection and extraction pipeline 2 is circular, and the diameter is 50 mu m; the side length of the groove 3 is smaller than one half of the diameter of the controlled liquid drop or particle; the diameter of the circular through hole 7 is smaller than the side length of the groove 3, and the diameter is 0.8mm.
Example 4
The digital control platform based on the surface tension is applied to the situation that the controlled carrier is particle, and comprises a rectangular control platform 1, a groove 3, a liquid supply pipeline 4, a liquid supply system total valve 5, a control valve 6, a circular through hole 7 and a support 8;
the rectangular control platform 1 is a concave plate platform with four side plates on the top surface, and the four corners of the bottom surface of the rectangular control platform 1 are uniformly provided with supports 8;
the bottom surface of the rectangular control platform 1 is provided with a plurality of evenly distributed grooves 3, the bottom center of each groove 3 is provided with a corresponding circular through hole 7, and the grooves 3 are communicated with the liquid supply pipeline 4 through the circular through holes 7; the liquid supply pipeline 4 consists of a main pipeline and a plurality of branches, one ends of the branches are respectively communicated with the circular through holes 7 through the control valve 6 and then are connected with the grooves 3, the other ends of the branches are connected to the main pipeline of the liquid supply pipeline 4, and the main pipeline of the liquid supply pipeline 4 is provided with a liquid supply system main valve 5;
when the digital control platform based on surface tension is used, a layer of liquid which is not compatible with controlled liquid drops or particles is paved in the rectangular control platform 1 to serve as a surface lubricant, the surface lubricant liquid level is immersed in the groove 3, and a surfactant capable of changing the surface tension of the surface lubricant liquid is introduced into the liquid supply pipeline 4.
The control valve 6 is a wireless control valve, the wireless control valve is connected with a valve control device, the valve control device is connected with a terminal server through a wireless communication network, the valve control device is used for receiving a command of the terminal server, sending back data, inquiring a valve state and controlling a valve function, and the terminal server is used for calculating the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point. The valve control device takes an 89C51 singlechip as a main control chip, can realize the functions of receiving a terminal server instruction, sending back data, inquiring valve state, controlling a valve and the like (the wireless remote valve control device described by CN201410307887.9 can be adopted), and the terminal server adopts software environments such as Visual basic, visual C++, labview and the like to calculate the optimal direction and the shortest path for the migration of the selected liquid film to reach the end point.
Wherein the groove 3 side length should be less than one half of the diameter of the controlled droplet or particle; the diameter of the circular through hole 7 is smaller than the side length of the groove 3, and the diameter is 0.8mm.
While the present invention has been described in detail with reference to the drawings, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (4)
1. The utility model provides a digital control platform based on surface tension which characterized in that: the device comprises a rectangular control platform (1), a liquid drop injection and extraction pipeline (2), a groove (3), a liquid supply pipeline (4), a liquid supply system total valve (5), a control valve (6), a circular through hole (7) and a support (8);
the rectangular control platform (1) is a concave plate platform with four side plates on the top surface, and the four corners of the bottom surface of the rectangular control platform (1) are uniformly provided with supports (8);
the four side plates of the rectangular control platform (1) are respectively and uniformly provided with a plurality of liquid drop injection and extraction pipelines (2) penetrating through the side plates, the liquid drop injection and extraction pipelines (2) on each side plate are equally spaced, are symmetrically distributed, the center line of the channel of the liquid drop injection and extraction pipeline (2) is vertical to the surface of the side plate of the rectangular control platform (1), and the outer side of the liquid drop injection and extraction pipeline (2) can be connected with a liquid drop injection device or an extraction device;
the bottom surface of the rectangular control platform (1) is provided with a plurality of evenly distributed grooves (3), the center of the bottom of each groove (3) is provided with a corresponding circular through hole (7), and the grooves (3) are communicated with the liquid supply pipeline (4) through the circular through holes (7); the liquid supply pipeline (4) consists of a main way and a plurality of branches, one ends of the branches are respectively communicated with the circular through holes (7) through the control valve (6) and then are connected with the grooves (3), the other ends of the branches are connected to the main way of the liquid supply pipeline (4), and the main way of the liquid supply pipeline (4) is provided with a main valve (5) of a liquid supply system;
when the digital control platform based on surface tension is used, a layer of liquid which is not compatible with controlled liquid drops or particles is paved in the rectangular control platform (1) to serve as a surface lubricant, the surface lubricant liquid level is immersed in the groove (3), and a surfactant capable of changing the surface tension of the surface lubricant liquid is introduced into the liquid supply pipeline (4);
the control valve (6) is a wireless control valve, the wireless control valve is connected with a valve control device, the valve control device is connected with a terminal server through a wireless communication network, the valve control device is used for receiving a command of the terminal server, sending back data, inquiring a valve state and controlling a valve function, and the terminal server is used for calculating an optimal direction and a shortest path for selecting liquid film migration to reach an end point;
the horizontal distance between the liquid drop injection and extraction pipelines (2) on each side plate of the rectangular control platform (1) is at least 6-10 times of the side length of the groove (3), the height of the liquid drop injection and extraction pipeline is greater than two thirds of the height of the rectangular control platform (1), and the instillation injection and extraction pipelines (2) penetrate through the inner side length of the side plate and at least reach the second row of grooves (3) of the rectangular control platform (1);
the section of the liquid drop injection and extraction pipeline (2) is circular, and the diameter is 50-700 mu m.
2. The surface tension based digital manipulation platform of claim 1, wherein: the groove (3) should have a side length smaller than one half of the diameter of the droplet or particle to be controlled.
3. The surface tension based digital manipulation platform of claim 1, wherein: the diameter of the circular through hole (7) is smaller than the side length of the groove (3), and the diameter is 0.1-1 mm.
4. A surface tension based digital manipulation platform according to any one of claims 1 to 3, wherein: when the controlled carrier is particles, the liquid drop injection and extraction pipeline (2) can be omitted.
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| WO2013115868A2 (en) * | 2011-11-04 | 2013-08-08 | President And Fellows Of Harvard College | Dynamic and switchable slippery surfaces |
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