CN109221257A - It prevents and treats large small moth dead wood and infuses dry bacterium solution and preparation method - Google Patents
It prevents and treats large small moth dead wood and infuses dry bacterium solution and preparation method Download PDFInfo
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- CN109221257A CN109221257A CN201811328976.6A CN201811328976A CN109221257A CN 109221257 A CN109221257 A CN 109221257A CN 201811328976 A CN201811328976 A CN 201811328976A CN 109221257 A CN109221257 A CN 109221257A
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- nutrient solution
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- small moth
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- Agronomy & Crop Science (AREA)
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Abstract
The present invention prevents and treats the dry bacterium solution of large small moth dead wood note and is formed by following components and weight: nutrient solution 60~78, stabilizer 5~17, muscardine 20~30；The present invention be select Huashan pine wither carpentery workshop be controlling object, bacterium solution is fixed in such a way that note is dry, microbial inoculum, which is not absorbed, slowly to be flowed outwardly instead into bark gap, spore will be propagated on entire trunk, the large small moth on trunk can not only be killed, the trunk, which also functions as, simultaneously passes spore body, and a large amount of spore can quickly be spread in entire woodland, be effectively controlled the diffusion of large small moth.Dry bacterium solution deinsectization significant effect is infused using dead wood, property is stablized, and prevention and treatment is long-acting, is protected from weather influences, and it is low in cost, it uses manpower and material resources sparingly, provides a kind of new way for prevention and treatment large small moth and other trunk borers, can be applicable in vast forest zone.
Description
Technical field
The present invention relates to a kind of insecticide and preparation method, specifically a kind of prevention and treatment large small moth dead wood infuses dry bacterium solution and preparation
Method belongs to plant protection field.
Background technique
Huashan pine is gained the name because concentrating hua-shan native to China, and it is flat to east that distributed areas are in China western part plateau
Former and low mountains and hills transition wide geographic area, many places are China the Changjiang river, Upper-middle Reaches of The Yellow River and Qinling-Dabashan region in mountain area area
One of the main composition tree species of forest and regeneration tree species and China's endemic tree have growth rapidly, and material is excellent etc.
Feature has important economic value and the ecological value.In recent years, with dendroctonus armandi for pioneer bark beetle in the Qinling Mountains
Bashan Mountain forest zone persistently occurs, and then induces other pest intrusions, causes huge economy and Ecological Loss.
In recent years, chemical prevention, biological control and pheromones application etc. are mainly used around dendroctonus armandi, chemistry is anti-
The method that emamection benaoate, flolimat, DDVP, imidacloprid and dinotefuran etc. spray that generally selects is controlled,
By various medicaments mix to reinforce control efficiency, also need sometimes by bark dig open spray or aggrieved wood cut down remove after dig skin
Spray, is limited to the weather of dendroctonus armandi generation area and the limitation of geographic factor, and medicament sprays that there are many drawbacks, no
A large amount of man power and material is only expended, and can cause environmental pollution；Biological control is that selection sprays muscardine, green muscardine fungus
Deng, but facility sprinkling need to be carried, extremely inconvenient, and inefficiency is carried in the woodland environment of mountainous region, once encountering rainy day, is sprayed
Applying the muscardine on trunk can be fallen by rain drop erosion, can not play a role at all；The optional timber quarantine of pheromones application,
The methods of repellent agent isolation, at present using relatively broad, but the objective reality of large small moth with can actually can not lure than column,
Aggravate the harm in aggrieved area still.So any single prevention and treatment at present is not significant effective, it need to be practical in conjunction with prevention and treatment area
Situation carries out integrated control using efficient medicament.
Summary of the invention
The purpose of the invention is to overcome defect present in current various prevention and treatment large small moth insecticides, and it is open a kind of
It prevents and treats large small moth dead wood and infuses dry bacterium solution and preparation method.
The present invention prevents and treats the dry bacterium solution of large small moth dead wood note and is formed by following components and weight:
Nutrient solution 60~78, stabilizer 5~17, muscardine 20~30；
Wherein nutrient solution is made of following components and parts by weight: 5 × 10^8 of sterile water, 7.5 × 10^6 of soybean powder, and sucrose 12.5 ×
10^6,7.5 × 10^5 of potassium dihydrogen phosphate, 2.5 × 10^5 of magnesium sulfate, 5 × 10^3 of calcium chloride, boric acid 15, four hydrated manganese sulfates 20,
Salzburg vitriol 40, two molybdic acid hydrate sodium 20, Zinc vitriol 200, Iron(III) chloride hexahydrate 250, VB115, VB27.5
VB61；Stabilizer is one or both of Tween-80 and sodium alginate mixture, and muscardine is beauveria bassiana, Bu Shi is white
Stiff bacterium and the one or more mixtures of Beauveria tenella；
Above-mentioned prevention and treatment large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. stabilizer is added into nutrient solution, survey pH value is 6.3-6.8,
C. finally addition muscardine, temperature are maintained at 25 DEG C -28 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
The present invention be select Huashan pine wither carpentery workshop be controlling object, utilize Huashan pine dead wood bark and xylem major part
Separation the characteristics of having lost transport capacity, in such a way that note is dry by bacterium solution it is fixed get on, microbial inoculum be not absorbed instead slowly to
Outer outflow enters bark gap, and spore will be propagated on entire trunk, can not only kill the large small moth on trunk, simultaneously should
Trunk, which also functions as, passes spore body, and a large amount of spore can quickly be spread in entire woodland, be effectively controlled the diffusion of large small moth.
Dry bacterium solution deinsectization significant effect is infused using dead wood, property is stablized, and prevention and treatment is long-acting, is protected from weather influences, and it is low in cost, save manpower
Material resources provide a kind of new way for prevention and treatment large small moth and other trunk borers, can be applicable in vast forest zone.
Specific embodiment
Below with reference to embodiment, the invention will be further described.
Embodiment one: prevention and treatment large small moth dead wood is infused dry bacterium solution and is formed by following components and weight:
Nutrient solution 64, Tween-80 12, beauveria bassiana 24；
Above-mentioned prevention and treatment large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. Tween-80 is added into nutrient solution, surveying pH value is 6.5,
C. finally addition muscardine, temperature are maintained at 25 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
Embodiment two: prevention and treatment large small moth dead wood is infused dry bacterium solution and is formed by following components and weight:
Nutrient solution 70, sodium alginate 10, beauveria bassiana 10, Beauveria tenella 10；
Above-mentioned prevention and treatment large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. sodium alginate is added into nutrient solution, surveying pH value is 6.5,
C. finally addition beauveria bassiana, Beauveria tenella, temperature are maintained at 25 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
Embodiment three: prevention and treatment large small moth dead wood is infused dry bacterium solution and is formed by following components and weight:
Nutrient solution 65, sodium alginate 5, beauveria bassiana 20, Beauveria tenella 10；
Prevent and treat large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. sodium alginate is added into nutrient solution, surveying pH value is 6.3,
C. finally addition beauveria bassiana, Beauveria tenella, temperature are maintained at 28 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
Example IV: prevention and treatment large small moth dead wood is infused dry bacterium solution and is formed by following components and weight:
Nutrient solution 66, sodium alginate 3, Tween-80 3, beauveria bassiana 10, Beauveria tenella 10, muscardine 8；
Prevent and treat large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. sodium alginate, Tween-80 3 are added into nutrient solution, surveying pH value is 6.5,
C. finally addition beauveria bassiana, Beauveria tenella, muscardine, temperature are maintained at 25 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
Embodiment five: prevention and treatment large small moth dead wood is infused dry bacterium solution and is formed by following components and weight:
Nutrient solution 70, sodium alginate 5, Tween-80 3, beauveria bassiana 12, muscardine 10；
Prevent and treat large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. sodium alginate, Tween-80 are added into nutrient solution, surveying pH value is 6.5,
C. it is finally added into beauveria bassiana, muscardine, temperature is maintained at 25 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
When infusing dry bacterium solution using present invention prevention and treatment large small moth dead wood, first with portable charged hand drill from earth's surface 60-
Hole of the oblique 45 ° of drill straights of 150cm butt through 4mm, deep 3~4cm, cuts off injection bottle pipe front end, is whittled into inclined plane shape, with point
Then medicine-pouring pipe is inserted into the tree hole being drilled by end or needle-penetration medicine injecting device blind hole, select to infuse according to trunk diameter of a cross-section of a tree trunk 1.3 meters above the ground size
The quantity of bacterium regularly replaces medicament.
Claims (6)
1. prevention and treatment large small moth dead wood is infused dry bacterium solution and is formed by following components and weight:
Nutrient solution 60~78, stabilizer 5~17, muscardine 20~30；
Its nutrient solution is made of following components and parts by weight: 5 × 10^8 of sterile water, 7.5 × 10^6 of soybean powder, sucrose 12.5 × 10
^6,7.5 × 10^5 of potassium dihydrogen phosphate, 2.5 × 10^5 of magnesium sulfate, 5 × 10^3 of calcium chloride, boric acid 15, four hydrated manganese sulfates 20, five
Hydrated copper sulfate 40, two molybdic acid hydrate sodium 20, Zinc vitriol 200, Iron(III) chloride hexahydrate 250, VB115, VB27.5
VB61；Stabilizer is one or both of Tween-80 and sodium alginate mixture, and muscardine is beauveria bassiana, Bu Shi is white
Stiff bacterium and the one or more mixtures of Beauveria tenella；
Above-mentioned prevention and treatment large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. stabilizer is added into nutrient solution, survey pH value is 6.3-6.8,
C. finally addition muscardine, temperature are maintained at 25 DEG C -28 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
2. prevention and treatment large small moth according to claim 1 infuses dry medicament, it is characterised in that by following components and weight
It forms:
Nutrient solution 64, Tween-80 12, beauveria bassiana 24；
Above-mentioned prevention and treatment large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. Tween-80 is added into nutrient solution, surveying pH value is 6.5,
C. finally addition muscardine, temperature are maintained at 25 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
3. prevention and treatment large small moth according to claim 1 infuses dry medicament, it is characterised in that by following components and weight
It forms:
Nutrient solution 70, sodium alginate 10, beauveria bassiana 10, Beauveria tenella 10；
Above-mentioned prevention and treatment large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. sodium alginate is added into nutrient solution, surveying pH value is 6.5,
C. finally addition beauveria bassiana, Beauveria tenella, temperature are maintained at 25 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
4. prevention and treatment large small moth according to claim 1 infuses dry medicament, it is characterised in that by following components and weight
It forms:
Nutrient solution 65, sodium alginate 5, beauveria bassiana 20, Beauveria tenella 10；
Prevent and treat large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. sodium alginate is added into nutrient solution, surveying pH value is 6.3,
C. finally addition beauveria bassiana, Beauveria tenella, temperature are maintained at 28 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
5. prevention and treatment large small moth according to claim 1 infuses dry medicament, it is characterised in that by following components and weight
It forms:
Nutrient solution 66, sodium alginate 3, Tween-80 3, beauveria bassiana 10, Beauveria tenella 10, muscardine 8；
Prevent and treat large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. sodium alginate, Tween-80 3 are added into nutrient solution, surveying pH value is 6.5,
C. finally addition beauveria bassiana, Beauveria tenella, muscardine, temperature are maintained at 25 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
6. prevention and treatment large small moth according to claim 1 infuses dry medicament, it is characterised in that by following components and weight
It forms:
Nutrient solution 70, sodium alginate 5, Tween-80 3, beauveria bassiana 12, muscardine 10；
Prevent and treat large small moth dead wood infuse dry bacterium solution the preparation method is as follows:
A. nutrient solution each component is simply mixed and is configured to nutrient solution,
B. sodium alginate, Tween-80 are added into nutrient solution, surveying pH value is 6.5,
C. it is finally added into beauveria bassiana, muscardine, temperature is maintained at 25 DEG C,
D. the bacterium solution prepared is dispensed into sharp mouth plastic bottle and is saved.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811328976.6A CN109221257A (en) | 2018-11-09 | 2018-11-09 | It prevents and treats large small moth dead wood and infuses dry bacterium solution and preparation method |
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| CN201811328976.6A CN109221257A (en) | 2018-11-09 | 2018-11-09 | It prevents and treats large small moth dead wood and infuses dry bacterium solution and preparation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004069A (en) * | 2019-05-14 | 2019-07-12 | 西南林业大学 | A kind of Strain of Beauveria bassiana and its application |
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| CN103535383A (en) * | 2013-10-31 | 2014-01-29 | 浙江农林大学天目学院 | Biopesticide containing natural fungus and preventing and treating monochamus alternatus in high-efficiency manner and application |
| CN105875653A (en) * | 2016-04-15 | 2016-08-24 | 佛山市聚成生化技术研发有限公司 | Microbial pesticide containing beauveria bassiana and preparation method of microbial pesticide |
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2018
- 2018-11-09 CN CN201811328976.6A patent/CN109221257A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535383A (en) * | 2013-10-31 | 2014-01-29 | 浙江农林大学天目学院 | Biopesticide containing natural fungus and preventing and treating monochamus alternatus in high-efficiency manner and application |
| CN105875653A (en) * | 2016-04-15 | 2016-08-24 | 佛山市聚成生化技术研发有限公司 | Microbial pesticide containing beauveria bassiana and preparation method of microbial pesticide |
Non-Patent Citations (3)
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| 李竹: "《物种战争之地道战》", 30 June 2015, 中国社会出版社 * |
| 聂虹珏等: ""用臼僵菌治松林大小蠹的研究"", 《研究性学习》 * |
| 赵明振: ""华山松大小蠹信息素筛选与活性检测"", 《中国博士学位论文全文数据库农业科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004069A (en) * | 2019-05-14 | 2019-07-12 | 西南林业大学 | A kind of Strain of Beauveria bassiana and its application |
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Application publication date: 20190118 |
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