CN109207388B - Preparation and application of glass fiber/high polymer composite immobilized microbial filler - Google Patents
Preparation and application of glass fiber/high polymer composite immobilized microbial filler Download PDFInfo
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- CN109207388B CN109207388B CN201710531167.4A CN201710531167A CN109207388B CN 109207388 B CN109207388 B CN 109207388B CN 201710531167 A CN201710531167 A CN 201710531167A CN 109207388 B CN109207388 B CN 109207388B
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- 239000003365 glass fiber Substances 0.000 title claims abstract description 51
- 239000000945 filler Substances 0.000 title claims abstract description 38
- 229920000642 polymer Polymers 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000002131 composite material Substances 0.000 title claims abstract description 12
- 230000000813 microbial effect Effects 0.000 title abstract description 4
- 241000894006 Bacteria Species 0.000 claims abstract description 88
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 80
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000002033 PVDF binder Substances 0.000 claims abstract description 13
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims abstract description 13
- 238000000576 coating method Methods 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 12
- 229920002492 poly(sulfone) Polymers 0.000 claims abstract description 12
- 239000011248 coating agent Substances 0.000 claims abstract description 11
- 239000002351 wastewater Substances 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 19
- 238000009630 liquid culture Methods 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 16
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 13
- 238000005286 illumination Methods 0.000 claims description 11
- 238000001179 sorption measurement Methods 0.000 claims description 11
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- 239000012153 distilled water Substances 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
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- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 229910052564 epsomite Inorganic materials 0.000 claims description 5
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 4
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- FXHOOIRPVKKKFG-UHFFFAOYSA-N N,N-Dimethylacetamide Chemical compound CN(C)C(C)=O FXHOOIRPVKKKFG-UHFFFAOYSA-N 0.000 claims description 2
- SECXISVLQFMRJM-UHFFFAOYSA-N N-Methylpyrrolidone Chemical compound CN1CCCC1=O SECXISVLQFMRJM-UHFFFAOYSA-N 0.000 claims description 2
- 241000190932 Rhodopseudomonas Species 0.000 claims description 2
- 239000011247 coating layer Substances 0.000 claims description 2
- 230000000593 degrading effect Effects 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 244000005700 microbiome Species 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 3
- 229920000098 polyolefin Polymers 0.000 abstract description 2
- 238000002834 transmittance Methods 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000002775 capsule Substances 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 description 12
- 230000015556 catabolic process Effects 0.000 description 8
- 238000006731 degradation reaction Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 5
- 238000005303 weighing Methods 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- 239000004697 Polyetherimide Substances 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000000852 hydrogen donor Substances 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 229920001601 polyetherimide Polymers 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
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Abstract
The invention discloses a preparation method of a glass fiber/high polymer composite immobilized microbial filler, belonging to the field of water treatment. The technical scheme of the invention is to provide a capsule type immobilized filler which is easy for the growth of microorganisms and has the characteristics of the immobilized filler, and the specific method comprises the following steps: (1) obtaining a high-efficiency photosynthetic bacteria strain through enrichment culture; (2) mechanical hot ligation of glass fiber balls; (3) photosynthetic bacteria are adsorbed and grown in the glass fiber balls; (4) preparing precursor solution of high polymer coating materials (polyvinylidene fluoride, polysulfone, polyolefins and the like); (5) and (3) synthesis of the photosynthetic bacteria immobilized filler. The filler prepared by the microorganism immobilization method has stronger mechanical property, and overcomes the problems of poor mass transfer performance and low light utilization rate of the traditional embedding filler due to the porosity and light transmittance of the glass fiber ball.
Description
Technical Field
The invention belongs to the technical field of water treatment, and particularly relates to a preparation method of an efficient fixed photosynthetic bacteria filler.
Background
Photosynthetic bacteria (PSB) are a type of microorganism that uses light as an energy source and can use organic matter, sulfide, ammonia, etc. in nature as a hydrogen donor and carbon source to perform photosynthesis under anaerobic illumination or aerobic dark conditions. Because photosynthetic bacteria have small thalli and are difficult to naturally settle, two problems of thallus loss and solid-liquid separation exist in practical application. In order to solve the two problems, fresh thalli need to be continuously cultured and added, and solid-liquid separation treatment work is also needed, so that the treatment process flow is complicated, the treatment cost is increased, and the popularization and the application of the microbial biomass in production are seriously influenced. The traditional immobilization method has a plurality of applications, but filler prepared by polyvinyl alcohol, sodium alginate, agar, gelatin and the like as embedding agents has poor mechanical properties, embedding materials can influence the diffusivity of substrates and products, most of the materials have poor mechanical properties and are easy to break, so that the application of the method is limited, and the adsorption method mostly uses porous materials as carriers to attach bacteria thereon, so that the bacteria are easy to fall off from the carriers under the action of water power. Therefore, the invention uses inorganic carrier material glass fiber ball as carrier, polyvinylidene fluoride or polysulfone as porous shell, the prepared immobilized filler not only has better mass transfer property, but also has high mechanical strength and is not easy to break.
Disclosure of Invention
The invention aims to solve the main problems in the prior bacterial immobilization, and provides an immobilized microorganism technology, wherein photosynthetic bacteria are adsorbed in a glass fiber ball by an adsorption method, and a layer of film is coated on the outer surface of the glass fiber ball by a coating method, so that an immobilized filler is prepared.
The purpose of the invention is realized by the following technical scheme:
a composite type photosynthetic bacteria immobilized filler is prepared by the following method:
(1) culturing photosynthetic bacteria in a photosynthetic bacteria liquid culture medium under the condition of illumination and anaerobic conditions until the concentration is 1-2g/L in terms of dry cell mass to obtain a photosynthetic bacteria enrichment culture solution, adding glass fiber balls, and adsorbing the photosynthetic bacteria in pore channel structures of the glass fiber balls through the adsorption action of the glass fiber balls to obtain the photosynthetic bacteria adsorption glass fiber balls;
(2) adding the high polymer into an organic solvent, stirring and dissolving at the temperature of 20-40 ℃ to obtain a coating reagent; the high polymer is polysulfone or polyolefin; the mass fraction of the high polymer in the coating reagent is 8-20%;
(3) and (2) immersing the glass fiber balls adsorbing the photosynthetic bacteria obtained in the step (1) in the coating reagent prepared in the step (2), stirring, shaking uniformly, taking out, placing in deionized water, standing to form a film-shaped porous coating layer on the surfaces of the glass fiber balls, taking out, and washing with distilled water to obtain the composite photosynthetic bacteria immobilized filler.
Further, the final concentration of the photosynthetic bacteria liquid culture medium of the invention comprises the following components: 0.5g/L yeast extract and 0.5g/L, CH peptone3COONa 3g/L、NH4Cl 0.1g/L、NaCl 0.5g/L、NaHCO3 0.5g/L、K2HPO4 0.2g/L、MgSO4·7H2O 0.1g/L、CaCl20.1g/L, water as solvent, and initial pH of 7-9.
Further, the preparation method of the glass fiber ball comprises the following steps: sterilizing glass fibers, performing mechanical hot ligation on the glass fibers to prepare spheres with uniform sizes, and cleaning and drying the spheres to obtain the glass fiber spheres.
Further, in the step (2) of the present invention, the organic solvent is N, N-dimethylformamide, N-dimethylacetamide, or N-methylpyrrolidone.
Further, in step (2) of the present invention, the polymer is preferably polyetherimide, polysulfone, polyethersulfone, polyacrylonitrile, polytetrafluoroethylene, or polyvinylidene fluoride.
Still further, the high polymer in the step (2) of the present invention is more preferably polysulfone or polyvinylidene fluoride.
Further, the volume of the photosynthetic bacteria enrichment culture solution in the step (1) is 100-1000 mL/g based on the mass of the added glass fiber balls.
Further, the solid-liquid separation in the step (1) is realized by centrifugation at the rotating speed of 10000-12000 rpm.
Further, the photosynthetic bacteria of the present invention are Rhodopseudomonas palustris photosynthetic bacteria.
Further, the invention provides an application of the composite photosynthetic bacteria immobilized filler in degrading high-salinity wastewater COD.
The invention has the beneficial effects that: the filler prepared by the microorganism immobilization method has stronger mechanical property, and overcomes the problems of poor mass transfer performance and low light utilization rate of the traditional embedding filler due to the porosity and light transmittance of the glass fiber ball.
Drawings
FIG. 1: example 1 an immobilized filler prepared from polyvinylidene fluoride (PVDF) and Polysulfone (PSF) of example 3;
FIG. 2: the degradation effect of the immobilized filler on COD.
FIG. 3: example 3 and comparative example 2 effect on COD degradation.
Detailed Description
The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto.
The photosynthetic bacteria of the present invention are preferably Rhodopseudomonas (Rhodopseudomonas palustris) photosynthetic bacteria, and the photosynthetic bacteria seed solution is purchased from Dinglong group of Zhejiang.
The sterile water adopted by the invention is deionized water treated by a high-pressure steam sterilization pot, and the treatment condition is that the temperature is 121 ℃ and the pressure is maintained for 30 minutes under the condition of 0.105 Mpa.
Example 1:
preparation of glass fiber balls
Sterilizing 3g of glass fiber in a high-pressure steam sterilization pot, then performing mechanical heat ligation on glass fiber balls with different filling densities to prepare glass fiber balls with uniform sizes, cleaning in sterile water, and drying to obtain the glass fiber balls.
Second, culture of photosynthetic bacteria
1. Configuring photosynthetic bacteria liquid culture medium
Weighing yeast extract 0.5g, peptone 0.5g, and CH3COONa 3g、NH4Cl 0.1g、NaCl 0.5g、NaHCO30.5g、K2HPO4 0.2g、MgSO4·7H2O 0.1g、CaCl2Adding 0.1g of the mixture into 1000mL of distilled water, adjusting the pH value to 7 by using 10% of Na0H and 10% of HCI in percentage by mass, and uniformly stirring to obtain the photosynthetic bacteria liquid culture medium.
2. Enrichment culture of photosynthetic bacteria
200ml of photosynthetic bacteria liquid culture medium is prepared, the photosynthetic bacteria liquid culture medium is poured into a 250 ml conical flask for high-pressure steam sterilization, wherein the sterilization temperature is 115 ℃, the sterilization time is 30 minutes, and the conical flask is taken out and cooled to the room temperature. Transferring photosynthetic bacteria stored in the 7-ring solid culture medium into a conical flask by using an inoculating ring, uniformly mixing, wrapping the periphery of the conical flask by using gauze, placing the conical flask into an incubator, and performing illumination anaerobic culture for 48 hours under the condition that the light intensity is 2000lux, wherein the culture temperature is 30 ℃, so as to obtain the photosynthetic bacteria enrichment culture solution.
Preparation of photosynthetic bacteria immobilized filler
1. Preparation of coating solution
And adding polyvinylidene fluoride into the N, N-dimethylformamide solution, stirring and dissolving to obtain the polyvinylidene fluoride solution.
2. Adsorption of glass fiber balls to bacteria
0.5g of glass fiber balls are weighed, washed clean, dried and added to 100mL of photosynthetic bacteria with the concentration of 1g/L for adsorption.
3. Preparation of immobilized fillers
And (3) placing the glass fiber balls adsorbed with 0.3g of bacteria in the prepared polyvinylidene fluoride solution, stirring, shaking uniformly, taking out, placing in deionized water, standing for 4min, taking out, and washing with distilled water.
Fourthly, simulating high-salinity wastewater indexes: salinity of 5%, COD: 2900 mg/L.
Fifth, activity detection of immobilized filler
0.5g of immobilized filler is put into 200mL of high-salinity wastewater and treated for 72h under the conditions that the temperature is 30 ℃ and the illumination intensity is 2000 lux.
Two sets of experiments were set up, the difference being that the concentration of the polymer in the coating solution was 8% and 20%, respectively, and it can be seen from fig. 2 that the degradation of the high salinity wastewater COD by the immobilized photosynthetic bacteria was 88% and 82%, respectively.
Example 2:
preparation of glass fiber balls
Sterilizing 3g of glass fiber in a high-pressure steam sterilization pot, then performing mechanical heat ligation on glass fiber balls with different filling densities to prepare glass fiber balls with uniform sizes, cleaning in sterile water, and drying to obtain the glass fiber balls.
Second, culture of photosynthetic bacteria
1. Configuring photosynthetic bacteria liquid culture medium
Weighing yeast extract 0.5g, peptone 0.5g, and CH3COONa 3g、NH4Cl 0.1g、NaCl 0.5g、NaHCO30.5g、K2HPO4 0.2g、MgSO4·7H2O 0.1g、CaCl2Adding 0.1g of the mixture into 1000mL of distilled water, adjusting the pH value to 7 by using 10% of Na0H and 10% of HCI in percentage by mass, and uniformly stirring to obtain the photosynthetic bacteria liquid culture medium.
2. Enrichment culture of photosynthetic bacteria
200ml of photosynthetic bacteria liquid culture medium is prepared, the photosynthetic bacteria liquid culture medium is poured into a 250 ml conical flask for high-pressure steam sterilization, wherein the sterilization temperature is 115 ℃, the sterilization time is 30 minutes, and the conical flask is taken out and cooled to the room temperature. Transferring photosynthetic bacteria stored in the 7-ring solid culture medium into a conical flask by using an inoculating ring, uniformly mixing, wrapping the periphery of the conical flask by using gauze, placing the conical flask into an incubator, and performing illumination anaerobic culture for 48 hours under the condition that the light intensity is 2000lux, wherein the culture temperature is 30 ℃, so as to obtain the photosynthetic bacteria enrichment culture solution.
Preparation of photosynthetic bacteria immobilized filler
4. Preparation of coating solution
And adding polyvinylidene fluoride into the N, N-dimethylformamide solution, stirring and dissolving to obtain the polyvinylidene fluoride solution.
5. Adsorption of glass fiber balls to bacteria
Weighing 0.5g of glass fiber balls, washing, drying, and adding photosynthetic bacteria with the concentration of 2g/L for adsorption.
6. Preparation of immobilized fillers
Calculating the mass of the adsorbed bacteria to be 0.3g by a mass difference method, placing the glass fiber balls in the prepared polyvinylidene fluoride solution, stirring, shaking uniformly, taking out, placing in deionized water, standing for 4min, taking out, and washing with distilled water.
Fourthly, simulating high-salinity wastewater indexes: salinity of 5%, COD: 2900 mg/L.
Fifth, activity detection of immobilized filler
0.5g of immobilized filler is put into 200mL of high-salinity wastewater and treated for 72h under the conditions that the temperature is 30 ℃ and the illumination intensity is 2000 lux.
Two groups of experiments are set, and the difference is that the concentration of the high polymer in the coating solution is respectively 8% and 20%, and as can be seen from figure 2, the degradation of the high salinity wastewater COD by the immobilized photosynthetic bacteria with the concentration of the high polymer of 8% can reach 89%; the degradation of the high salinity wastewater COD by the immobilized photosynthetic bacteria with the high polymer concentration of 20 percent can reach 78 percent.
Comparative example 1:
cultivation of photosynthetic bacteria
1. Configuring photosynthetic bacteria liquid culture medium
Weighing yeast extract 0.5g, peptone 0.5g, and CH3COONa 3g、NH4Cl 0.1g、NaCl 0.5g、NaHCO30.5g、K2HPO4 0.2g、MgSO4·7H2O 0.1g、CaCl2Adding 0.1g of the mixture into 1000mL of distilled water, adjusting the pH value to 7 by using 10% of Na0H and 10% of HCI in percentage by mass, and uniformly stirring to obtain the photosynthetic bacteria liquid culture medium.
2. Enrichment culture of photosynthetic bacteria
200ml of photosynthetic bacteria liquid culture medium is prepared, the photosynthetic bacteria liquid culture medium is poured into a 250 ml conical flask for high-pressure steam sterilization, wherein the sterilization temperature is 115 ℃, the sterilization time is 30 minutes, and the conical flask is taken out and cooled to the room temperature. Transferring photosynthetic bacteria stored in the 7-ring solid culture medium into a conical flask by using an inoculating ring, uniformly mixing, wrapping the periphery of the conical flask by using gauze, placing the conical flask in an incubator, and performing illumination anaerobic culture for 48 hours under the condition that the light intensity is 2000lux, wherein the culture temperature is 30 ℃, so as to obtain the photosynthetic bacteria enrichment culture solution.
Secondly, simulating high-salinity wastewater indexes: salinity of 5%, COD: 2900 mg/L.
Thirdly, photosynthetic bacteria degrade high-salinity wastewater
0.3g of photosynthetic bacteria is put into 200mL of high-salinity wastewater and treated for 72 hours under the conditions that the temperature is 30 ℃ and the illumination intensity is 2000 lux.
As can be seen from FIG. 2, the suspended photosynthetic bacteria can degrade the COD of the high-salinity wastewater by 63%. Obviously lower than the degradation of the immobilized filler to COD.
Example 3:
firstly, preparing glass fiber balls: in accordance with example 1
Secondly, culturing photosynthetic bacteria: in accordance with example 1
Preparation of photosynthetic bacteria immobilized filler
1. Adding polysulfone (molecular weight: 65000Da) into N, N-dimethylformamide solution, stirring, and dissolving to obtain polysulfone solution; wherein the mass fraction of the polysulfone is 10%.
2. Adsorption of glass fiber balls to bacteria: in accordance with example 1
3. Preparation of immobilized fillers
Calculating the mass of the adsorbed bacteria to be 0.3g by a mass difference method, placing the glass fiber balls in the prepared polysulfone solution, stirring, shaking uniformly, taking out, placing in deionized water, standing for 3min, taking out, and washing with distilled water.
Fourthly, simulating indexes of the high-salinity wastewater: salinity of 7%, COD: 2900 mg/L.
Fourth, activity detection of immobilized filler
0.5g of immobilized filler is put into 200mL of high-salinity wastewater and treated for 72h under the conditions that the temperature is 30 ℃ and the illumination intensity is 2000 lux.
The degradation of the immobilized photosynthetic bacteria to the COD of the salt-containing wastewater can reach 91 percent.
Comparative example 2:
firstly, culturing photosynthetic bacteria: in accordance with example 3
Secondly, simulating high-salinity wastewater indexes: salinity of 7%, COD: 2900 mg/L.
Thirdly, photosynthetic bacteria degrade high-salinity wastewater
0.3g of photosynthetic bacteria is put into 200mL of high-salinity wastewater and treated for 72 hours under the conditions that the temperature is 30 ℃ and the illumination intensity is 2000 lux.
The degradation of suspended photosynthetic bacteria to the COD of the salt-containing wastewater is 75 percent.
Claims (5)
1. The composite photosynthetic bacteria immobilized filler is characterized by being prepared by the following method:
(1) culturing photosynthetic bacteria in a photosynthetic bacteria liquid culture medium under the condition of illumination and anaerobic conditions until the concentration is 1-2g/L in terms of dry cell mass to obtain a photosynthetic bacteria enrichment culture solution, adding glass fiber balls, and adsorbing the photosynthetic bacteria in pore channel structures of the glass fiber balls through the adsorption action of the glass fiber balls to obtain the photosynthetic bacteria adsorption glass fiber balls; the preparation method of the glass fiber ball comprises the following steps: sterilizing glass fibers, performing mechanical hot ligation on the glass fibers to prepare spheres with uniform sizes, and cleaning and drying the spheres to obtain the glass fiber spheres;
(2) adding the high polymer into an organic solvent, stirring and dissolving at the temperature of 20-40 ℃ to obtain a coating reagent; the mass fraction of the high polymer in the coating reagent is 8-20%; the organic solvent in the step (2) is N, N-dimethylformamide, N-dimethylacetamide or N-methylpyrrolidone; the high polymer is polysulfone or polyvinylidene fluoride;
(3) and (2) immersing the glass fiber balls adsorbing the photosynthetic bacteria obtained in the step (1) in the coating reagent prepared in the step (2), stirring, shaking uniformly, taking out, placing in deionized water, standing to form a film-shaped porous coating layer on the surfaces of the glass fiber balls, taking out, and washing with distilled water to obtain the composite photosynthetic bacteria immobilized filler.
2. The composite photosynthetic bacteria immobilized filler of claim 1, wherein: the final concentration of the photosynthetic bacteria liquid culture medium is as follows: 0.5g/L yeast extract and 0.5g/L, CH peptone3COONa 3 g/L、NH4Cl 0.1 g/L、NaCl 0.5 g/L、NaHCO30.5 g/L、K2HPO4 0.2 g/L、MgSO4·7H2O 0.1 g/L、CaCl20.1g/L, water as solvent, and initial pH of 7-9.
3. The composite photosynthetic bacteria immobilized filler of claim 1, wherein: the volume of the photosynthetic bacteria enrichment culture solution in the step (1) is 100-1000 mL/g based on the mass of the added glass fiber balls.
4. The composite photosynthetic bacteria immobilized filler of claim 1, wherein: the photosynthetic bacteria are Rhodopseudomonas (Rhodopseudomonas palustris) photosynthetic bacteria.
5. The use of the composite photosynthetic bacteria immobilized filler of claim 1 in degrading high-salinity wastewater COD.
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101353631A (en) * | 2007-07-27 | 2009-01-28 | 中国科学院沈阳应用生态研究所 | Cultivation method of Rhodopseudomonas sphaeroides for sea cucumber |
CN204455076U (en) * | 2015-01-30 | 2015-07-08 | 杨晓庆 | A kind of immobilized microorganism device for sewage disposal |
-
2017
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101353631A (en) * | 2007-07-27 | 2009-01-28 | 中国科学院沈阳应用生态研究所 | Cultivation method of Rhodopseudomonas sphaeroides for sea cucumber |
CN204455076U (en) * | 2015-01-30 | 2015-07-08 | 杨晓庆 | A kind of immobilized microorganism device for sewage disposal |
Non-Patent Citations (3)
Title |
---|
Confined encapsulation of living cells in self-assembly fiber macrospheres with micro/nanoporous polymer shell for transformation of contaminants to green energy;Lei Qin,et al;《Journal of Materials Chemistry A》;20191218;1-10 * |
Mesoporous Silica Spheres as Supports for Enzyme Immobilization and Encapsulation;Yajun Wang,et al;《Chem. Mater.》;20050204;第17卷;953-961 * |
固定化微生物技市研究进展及其在印染废水处理巾的应用;刘荣荣 等;《印染助剂》;20140331;第31卷(第3期);1-5 * |
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