CN109187148A - A kind of pathological tissue specimen fixer - Google Patents
A kind of pathological tissue specimen fixer Download PDFInfo
- Publication number
- CN109187148A CN109187148A CN201811007167.5A CN201811007167A CN109187148A CN 109187148 A CN109187148 A CN 109187148A CN 201811007167 A CN201811007167 A CN 201811007167A CN 109187148 A CN109187148 A CN 109187148A
- Authority
- CN
- China
- Prior art keywords
- tissue specimen
- fixer
- pathological tissue
- pathological
- hpo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000001575 pathological effect Effects 0.000 title claims abstract description 54
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 36
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 35
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 12
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 12
- 229910000397 disodium phosphate Inorganic materials 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims abstract description 12
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims abstract description 12
- 229910000162 sodium phosphate Inorganic materials 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 9
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
- 238000003915 air pollution Methods 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract description 3
- 230000003834 intracellular effect Effects 0.000 abstract description 3
- 238000003908 quality control method Methods 0.000 abstract description 3
- 238000010186 staining Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 238000000338 in vitro Methods 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 5
- 230000007170 pathology Effects 0.000 description 3
- 239000000834 fixative Substances 0.000 description 2
- 238000010827 pathological analysis Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Abstract
The present invention relates to pathological tissue specimen fixer, the component and proportion for including are as follows: formalin solution: 90-130ml;Na2HPO4•12H2O:13-17g；NaH2PO4•2H2O:4.4-4.7g;NaCL:8-10g;Acetic acid: 27-32ml.The present invention keeps pathological tissue specimen fixation simple and convenient, and can significantly improve the fixed effect of pathological tissue, reduces the influence to intracellular reactive substance.This product is prepackage fixer, without preparing, twist-off closure puts down sample, largely facilitates the operation sequence of lab assistant or medical staff, avoids air pollution, and play the role of standardization and standardized, the sample quality control before being conducive to staining analysis.
Description
Technical field
The present invention relates to a kind of pathological tissue specimen fixers, belong to sample disposal technical field.
Background technique
Pathology technique is the basis of pathological diagnosis, and modern pathological diagnosis is increasingly dependent on the development of pathology technique.With point
The fast development of sub- pathology technique, the requirement to tissue fixed effect are higher and higher.The quality of pathological section manufacturing technology is related to
Multiple links, wherein the fixation of pathological tissue is a most important step, and the step that can not most remedy during entire film-making.
Pathological tissue is fixed refer to using some chemical reagent handle tissue by save tissue or cell position, in the form of and structure.
Traditional tissue fixative solution uses formalin, precipitates through the more suitable chromogenesis of the fixed outmoded tissue of formalin,
The acidic materials that storage generates for a long time will affect the fixation of tissue, the dyeing of nucleus and the preservation of tissue antigen.
Summary of the invention
The technical problem to be solved by the present invention is in view of the above-mentioned drawbacks in the prior art, provide a kind of instant, solid
Determine the good pathological tissue specimen fixer of effect.
In order to solve the above technical problems, a kind of pathological tissue specimen fixer provided by the invention, it is characterised in that packet
The component and proportion contained is as follows:
Formalin solution: 90-130ml;
Na2HPO4•12H2O:13-17g;
NaH2PO4•2H2O:4.4-4.7g;
NaCL:8-10g;
Acetic acid: 27-32ml.
As further embodiment, the pathological tissue specimen fixer, the component for including and proportion it is as follows:
Formalin solution: 100-120ml;
Na2HPO4•12H2O:14-16g;
NaH2PO4•2H2O:4.4-4.6g;
NaCL:8.5-10g;
Acetic acid: 28-31ml.
As further scheme, the pathological tissue specimen fixer, the component for including and proportion it is as follows:
Formalin solution: 120ml;
Na2HPO4•12H2O:15g;
NaH2PO4•2H2O:4.5g;
NaCL:8.5g;
Acetic acid: 30ml.
Pathological tissue specimen fixer of the present invention also further characterized in that
1, the pH value of pathological tissue specimen fixer is 7.0 ± 0.2.
2, the mass percent concentration of formaldehyde is 37%-40% in formalin solution.
The invention further relates to the application methods of above-mentioned pathological tissue specimen fixer, it is characterised in that: basis when in use
The size selection of tissue specimen to be fixed saves the specification of container, opens the container, tissue specimen is put into, make tissue specimen
It is completely submerged in fixer, closed container, stands 2-12h, that is, complete the fixation of tissue specimen.
Further, the container is fixed liquid device or sample storing bottle.
Further, the volume of the fixer and tissue specimen volume ratio are greater than or equal to 4.
Further, after opening the container, being put into for tissue specimen is completed in 10 minutes.
Compared with prior art, the beneficial effects of the present invention are:
The present invention keeps pathological tissue specimen fixation simple and convenient, and can significantly improve the fixed effect of pathological tissue, reduces to thin
The influence of intracellular activity substance.This product is prepackage fixer, and without preparing, twist-off closure puts down sample, in very great Cheng
Facilitate the operation sequence of lab assistant or medical staff on degree, avoid air pollution, and play standardization and it is standardized
Effect, the sample quality control before being conducive to staining analysis.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, right With reference to embodiment
The technical solution of this patent is described in further details.
Embodiment 1
The present embodiment pathological tissue specimen fixer, according to comprising component and proportion it is as follows: the formalin of 40% concentration is molten
Liquid: 90ml, Na2HPO4•12H2O:13g, NaH2PO4•2H2O:4.4g, NaCL:8g, acetic acid 27ml；The above ingredient mixes molten
Liquid, as pathological tissue specimen fixer.Pathological tissue specimen fixer is stored in fixed liquid device, for fixed and temporary
Shi Cunfang pathological tissue specimen.
The present embodiment pathological tissue specimen fixer selects fixer when in use, according to the size of tissue specimen to be fixed
The specification of device unscrews fixer device bottle cap, in vitro tissue is immediately placed in fixed liquid device (in 10min), fixed
Liquid and tissue specimen volume ratio are not less than 4 times, and tissue specimen should be completely submerged in fixer, screw bottle cap, and the set time is
2-12h。
Embodiment 2
The present embodiment pathological tissue specimen fixer, according to comprising component and proportion it is as follows: the formalin of 37% concentration is molten
Liquid: 100ml, Na2HPO4•12H2O:14g, NaH2PO4•2H2O:4.5g, NaCL:8.5g, acetic acid 28ml；The mixing of above ingredient and
In vitro pathological tissue specimen is placed in fixer as fixative for tissue sample at solution, for fixed and temporarily storage
Pathological tissue specimen.
The present embodiment pathological tissue specimen fixer when in use, the specification of sample storing bottle is selected according to tissue size,
Sample storing bottle is opened, after pathological tissue specimen fixer is imported, in vitro tissue is immediately placed in sample storing bottle and is carried out
Fixed (in 10min), fixer and tissue specimen volume ratio are not less than 4 times, and tissue specimen should be completely submerged in fixer,
Seal sheet glass storing bottle, set time 2-12h.
Embodiment 3
The present embodiment pathological tissue specimen fixer, according to comprising component and proportion it is as follows: the formalin of 40% concentration is molten
Liquid: 110ml, Na2HPO4•12H2O:15g, NaH2PO4•2H2O:4.6g, NaCL:9g, acetic acid 29ml；The above ingredient mixes
Solution, as pathological tissue specimen fixer.Pathological tissue specimen fixer is stored in fixed liquid device, for fixed and
Temporarily storage pathological tissue specimen.
The present embodiment pathological tissue specimen fixer selects fixer when in use, according to the size of tissue specimen to be fixed
The specification of device unscrews fixer device bottle cap, in vitro tissue is immediately placed in fixed liquid device (in 10min), fixed
Liquid and tissue specimen volume ratio are not less than 4 times, and tissue specimen should be completely submerged in fixer, screw bottle cap, and the set time is
2-12h。
Embodiment 4
The present embodiment pathological tissue specimen fixer, according to comprising component and proportion it is as follows: the formalin of 40% concentration is molten
Liquid: 120ml, Na2HPO4•12H2O:16g, NaH2PO4•2H2O:4.65g, NaCL:9.5g, acetic acid 30ml；The above ingredient mixing
Into solution, as pathological tissue specimen fixer.Pathological tissue specimen fixer is stored in fixed liquid device, for solid
Fixed and temporary storage pathological tissue specimen.
The present embodiment pathological tissue specimen fixer selects fixer when in use, according to the size of tissue specimen to be fixed
The specification of device unscrews fixer device bottle cap, in vitro tissue is immediately placed in fixed liquid device (in 10min), fixed
Liquid and tissue specimen volume ratio are not less than 4 times, and tissue specimen should be completely submerged in fixer, screw bottle cap, and the set time is
2-12h。
Embodiment 5
The present embodiment pathological tissue specimen fixer, according to comprising component and proportion it is as follows: the formalin of 40% concentration is molten
Liquid: 130ml, Na2HPO4•12H2O:17g, NaH2PO4•2H2O:4.7g, NaCL:10g, acetic acid 31ml；The mixing of above ingredient and
At solution, as pathological tissue specimen fixer.Pathological tissue specimen fixer is stored in fixed liquid device, for fixing
With temporary storage pathological tissue specimen.
The present embodiment pathological tissue specimen fixer selects fixer when in use, according to the size of tissue specimen to be fixed
The specification of device unscrews fixer device bottle cap, in vitro tissue is immediately placed in fixed liquid device (in 10min), fixed
Liquid and tissue specimen volume ratio are not less than 4 times, and tissue specimen should be completely submerged in fixer, screw bottle cap, and the set time is
2-12h。
The present invention keeps pathological tissue specimen fixation simple and convenient, and can significantly improve the fixed effect of pathological tissue, reduces
Influence to intracellular reactive substance.This product is prepackage fixer, and without preparing, plug and play largely facilitates
The operation sequence of lab assistant or medical staff avoids air pollution, and plays the role of standardization and standardized, favorably
Sample quality control before staining analysis.
In addition to the implementation, the present invention can also have other embodiments.It is all to use equivalent substitution or equivalent transformation shape
At technical solution, fall within the scope of protection required by the present invention.
Claims (9)
1. a kind of pathological tissue specimen fixer, it is characterised in that the component and proportion for including are as follows:
Formalin solution: 90-130ml;
Na2HPO4•12H2O:13-17g;
NaH2PO4•2H2O:4.4-4.7g;
NaCL:8-10g;
Acetic acid: 27-32ml.
2. pathological tissue specimen fixer according to claim 1, it is characterised in that the component and proportion for including are as follows:
Formalin solution: 100-120ml;
Na2HPO4•12H2O:14-16g;
NaH2PO4•2H2O:4.4-4.6g;
NaCL:8.5-10g;
Acetic acid: 28-31ml.
3. pathological tissue specimen fixer according to claim 1, it is characterised in that the component and proportion for including are as follows:
Formalin solution: 120ml;
Na2HPO4•12H2O:15g;
NaH2PO4•2H2O:4.5g;
NaCL:8.5g;
Acetic acid: 30ml.
4. any pathological tissue specimen fixer according to claim 1, it is characterised in that: pH value is 7.0 ± 0.2.
5. pathological tissue specimen fixer according to claim 1 to 4, it is characterised in that: first in formalin solution
The mass percent concentration of aldehyde is 37%-40%.
6. the application method of pathological tissue specimen fixer described in claim 5, it is characterised in that: when in use according to be fixed
The size selection of tissue specimen saves the specification of container, opens the container, tissue specimen is put into, soak tissue specimen completely
Not in fixer, closed container stands 2-12h, that is, completes the fixation of tissue specimen.
7. the application method of pathological tissue specimen fixer according to claim 6, it is characterised in that: the container is to fix
Liquid device or sample storing bottle.
8. the application method of pathological tissue specimen fixer according to claim 6, it is characterised in that: the body of the fixer
It is long-pending to be greater than or equal to 4 with tissue specimen volume ratio.
9. the application method of pathological tissue specimen fixer according to claim 6, it is characterised in that: open the container
Afterwards, being put into for tissue specimen is completed in 10 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811007167.5A CN109187148A (en) | 2018-08-31 | 2018-08-31 | A kind of pathological tissue specimen fixer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811007167.5A CN109187148A (en) | 2018-08-31 | 2018-08-31 | A kind of pathological tissue specimen fixer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109187148A true CN109187148A (en) | 2019-01-11 |
Family
ID=64917105
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811007167.5A Pending CN109187148A (en) | 2018-08-31 | 2018-08-31 | A kind of pathological tissue specimen fixer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109187148A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020182653A1 (en) * | 2001-05-29 | 2002-12-05 | Shigeki Namimatsu | Antigen activating method and antigen activator |
| CN102062709A (en) * | 2010-12-13 | 2011-05-18 | 苏州大学 | Method for quickly slicing fish tissues |
| CN104304414A (en) * | 2014-11-18 | 2015-01-28 | 苏州大福外贸食品有限公司 | Method for processing quick-frozen onions |
| CN105454219A (en) * | 2015-12-14 | 2016-04-06 | 浙江检康生物技术股份有限公司 | Stationary liquid for preserving pathological tissues |
| CN107375509A (en) * | 2017-08-04 | 2017-11-24 | 河南省科高植物天然产物开发工程技术有限公司 | It is a kind of to be used to improve immunity, the health food of pre- anti-cancer |
-
2018
- 2018-08-31 CN CN201811007167.5A patent/CN109187148A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020182653A1 (en) * | 2001-05-29 | 2002-12-05 | Shigeki Namimatsu | Antigen activating method and antigen activator |
| CN102062709A (en) * | 2010-12-13 | 2011-05-18 | 苏州大学 | Method for quickly slicing fish tissues |
| CN104304414A (en) * | 2014-11-18 | 2015-01-28 | 苏州大福外贸食品有限公司 | Method for processing quick-frozen onions |
| CN105454219A (en) * | 2015-12-14 | 2016-04-06 | 浙江检康生物技术股份有限公司 | Stationary liquid for preserving pathological tissues |
| CN107375509A (en) * | 2017-08-04 | 2017-11-24 | 河南省科高植物天然产物开发工程技术有限公司 | It is a kind of to be used to improve immunity, the health food of pre- anti-cancer |
Non-Patent Citations (1)
| Title |
|---|
| 曹江: "介绍一种***组织切片的优良固定液", 《浙江肿瘤通讯》 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190111 |
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| RJ01 | Rejection of invention patent application after publication |