CN109182152A - For improving the culture medium of aweto mycelium active constituent - Google Patents
For improving the culture medium of aweto mycelium active constituent Download PDFInfo
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Abstract
The invention discloses a kind of for improving the culture medium of aweto mycelium active constituent.Described includes: potato juice, nutrient powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, Radix Sophorae Flavescentis extractive liquid, Chinese medical stone water for improving the culture medium of aweto mycelium active constituent.Compared with prior art, provided by the present invention for improving the culture medium of aweto mycelium active constituent, the cultivation cycle that cordyceps sinensis can be shortened significantly improves the content of Cordyceps sinensis polysaccharide in aweto mycelium, cordycepic acid, cordycepin and adenosine, has high nutritive value.
Description
Technical field
The present invention relates to a kind of for improving the culture medium of aweto mycelium active constituent.
Background technique
Cordyceps Eumycota, Ascomycotina, gang pyrenomycetes, ergot Zoopagales, Clavicipitaceae Cordyceps.Has strengthening the essence
Gas, qi-restoratives damage, relieving cough and reducing sputum effect.Have immunological regulation, antitumor, increasing leucocyte, blood pressure lowering, anti-aging, it is antifatigue, adjust in
The multiple pharmacological effects such as secretion, antibacterial, antiviral, are considered as precious strengthening by means of tonics Chinese medicine since ancient times.It is by ergot
Cordyceps sinensis fungi, that is, Hirsutella hepiali Chen et Shen of Cordycepps Cordyceps parasitizes the bat moth larvae in alpine meadow soil, keeps larva stiff
Change, under optimum conditions, summer goes out long rod-shaped stroma by bombys batryticatus head end pumping and forms (the i.e. fructification of cordyceps sinensis fungi
The complex constituted with bombys batryticatus mycelia core (larva corpse)).It mainly originates in China Qinghai, Tibet, Sichuan, Yunnan, Gansu etc.
The severe cold areas and snow-capped mountains and marshlands of provinces and regions height above sea level 3500-5000m.Modern research shows that cultural mycelium and natural winter worm
Summer grass has similar chemical component, pharmacological action and clinical efficacy, and toxicity is smaller than natural, this is artificial culture Chinese caterpillar fungus hypha
Body replaces natural cordyceps to provide foundation.
Hirsutella hepiali Chen et Shen is the bat of isolated section ergot fungus cordyceps sinensis from fresh " cordyceps sinensis "
Moth coat spore strain, which has been included in through Ministry of Health of the People's Republic of China's approval " can be used for the fungi strain of health food
List " is natural " cordyceps sinensis " artificial substituting product, has " invigorating the lung and the kidney, hemostasis and phlegm function.For chronic cough and dyspnea of deficiency type, overstrain cough
Hemoptysis, impotence and seminal emission, soreness of waist and knee joint ".Modern medicine discovery also has calm, anticonvulsion, decompression, improves myocardial ischemia, is anti-ageing
Always, improve immunity, bidirectional modulation human endocrine, it is anti-rejection and anti-lung cancer, lymph cancer, liver cancer effect, medical value
Increasingly cause the attention of domestic and international pharmacy circle.Drug currently on the market containing Hirsutella hepiali Chen et Shen powder have Bailing capsule,
Hundred enable piece, hundred that particle, hundred is enabled to enable chewable tablets.Health food containing Hirsutella hepiali Chen et Shen powder have Hirsutella hepiali Chen et Shen powder,
Hirsutella hepiali Chen et Shen silk gum capsule etc..The cultivation cycle of aweto mycelium is long in the prior art, aweto mycelium
Quality is irregular, and active component content is relatively low, does not have the effect of natural cs.
Summary of the invention
Aiming at the above shortcomings existing in the prior art, technical problem to be solved by the invention is to provide one kind for mentioning
The culture medium of high aweto mycelium active constituent, can significantly improve Cordyceps sinensis polysaccharide in aweto mycelium, cordycepic acid,
The content of cordycepin and adenosine has high nutritive value.
Specific technical solution is as follows:
It is a kind of for improving the culture medium of aweto mycelium active constituent, including following raw material: potato juice, nutrition
Powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, Chinese medical stone water.
Another technical solution, a kind of for improving the culture medium of aweto mycelium active constituent, including following raw material:
Potato juice, nutrient powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, Radix Sophorae Flavescentis extractive liquid, Chinese medical stone water.
Preferably, the culture medium for being used to improve aweto mycelium active constituent, by the original of following weight parts
Material is mixed to get: 4-8 parts of potato juice, 2-6 parts of nutrient powder, 1-3 parts of glucose, 0.1-0.5 parts of peptone, potassium dihydrogen phosphate
0.02-0.08 parts, 0.02-0.06 parts of magnesium sulfate, 0.5-1 parts of Radix Sophorae Flavescentis extractive liquid, 120-180 parts of Chinese medical stone water.
The potato juice is prepared by the following method to obtain, and described part is parts by weight: by peeling potatoes, bud eye is removed,
With potato residues are obtained after broken crusher machine, 8-12 parts of potato residues and 30-50 parts of water are mixed, in 90-100 DEG C of heat preservation 40-
60 minutes, using 200-300 mesh filter-cloth filtering, obtain potato juice.
The nutrient powder is Hericium erinaceus extract and/or egg albumen powder.Preferably, the nutrient powder is Hericium erinaceus extraction
The mixture of object and egg albumen powder, wherein the mass ratio of the Hericium erinaceus extract and egg albumen powder is (1-5): (1-5).
The Hericium erinaceus extract is prepared by the following method to obtain, and described part is parts by weight: by Hericium erinaceus at 40-60 DEG C
Dry to constant weight, crushing crosses 60-100 mesh, obtains hedgehog hydnum mushroom powder, and 5-10 parts of hedgehog hydnum mushroom powders and 100-150 parts of water are mixed,
With 100-300 revs/min stirring 5-10 minutes, add 0.02-0.06 parts of acid proteases and 0.05-0.1 parts of cellulases, use
The salt acid for adjusting pH that mass fraction is 10% is 3.5-4.5, then 35-45 DEG C with 100-300 revs/min stirring 1-3 hours, obtain
Enzymolysis liquid, by enzymolysis liquid 90-100 DEG C heat preservation 5-10 minutes, then with 3000-5000 revs/min centrifugation 10-20 minutes, supernatant
The density that liquid is concentrated under reduced pressure into 40 DEG C is 1.08-1.12g/mL, then is spray-dried, and Hericium erinaceus extract is obtained.
The Chinese medical stone water is prepared by the following method to obtain, and described part is parts by weight: by 1 part of medical stone and 20-40 parts
Water mixing, 20-30 DEG C standing 20-30 minutes, be then sonicated 10-20 minutes, using 800 mesh filter-cloth filterings, filtrate is wheat
Meal stone water；The condition of the ultrasonic treatment are as follows: 40-60 DEG C of ultrasonic temperature, supersonic frequency 25-35KHz, ultrasonic power 100-
150W。
The preparation method of the Radix Sophorae Flavescentis extractive liquid includes the following steps that described part is parts by weight:
(1) kuh-seng is crushed, crosses 20-50 mesh, obtains kuh-seng powder, 5-10 parts of kuh-seng powder and 60-100 parts of water are mixed,
0.01-0.1 parts of mixed enzyme is added, it is 5.5-6.5 that the aqueous citric acid solution for being 10% with mass fraction, which adjusts pH, in 40-50
DEG C with 100-300 revs/min stirring 1-3 hours, obtain enzymolysis liquid；
(2) enzymolysis liquid 85-95 DEG C refluxing extraction 60-90 minutes, using 300 mesh filter-cloth filterings, obtain filtrate and filter residue, filter
40-80 part water are added in slag, 85-95 DEG C refluxing extraction 40-60 minutes, using 300 mesh filter-cloth filterings, merging filtrate twice,
The density for being concentrated under reduced pressure into 50 DEG C is 1.08-1.12g/mL, obtains Radix Sophorae Flavescentis extractive liquid.
The mixed enzyme is the cellulase of 70-80wt% and the mixture of 20-30wt% alpha-amylase.The present invention also mentions
The method with above-mentioned aweto mycelium culture aweto mycelium is supplied.It is specific as follows:
A kind of preparation method of aweto mycelium, comprising the following steps:
(1) inoculated and cultured: 2000mL conical flask is packed into 800-1200mL, and above-mentioned to be used to improve aweto mycelium living
The culture medium of property ingredient, 120-125 DEG C sterilizing 20-30 minutes, be cooled to 20-30 DEG C, be aseptically inoculated with bat moth
Coat spore strain, on 18-25 DEG C of constant-temperature table culture to mycelial concentration be 30-35wt%, shaking speed be 150-180 turn/
Point, obtain conical flask strain cultivation liquid；
(2) it seed tank culture: is packed into 200L fermentor above-mentioned for improving aweto mycelium active constituent
Culture medium 80-120L, 120-125 DEG C sterilizing 20-30 minutes, be cooled to 20-30 DEG C, conical flask strain cultivation liquid added
Enter in fermentor, is 18-25 DEG C in temperature, mixing speed is cultivated 8-12 days under the conditions of being 160-200 revs/min, in fermentation process
Tank presses 0.04-0.06MPa, and the ventilatory capacity of filtrated air is (0.5-1) vvm, obtains seed tank culture liquid；
(3) it fermentation tank culture: is packed into the fermentor of 2000L above-mentioned for improving aweto mycelium active constituent
Culture medium 1000-1300L, 120-125 DEG C sterilizing 20-30 minutes, be cooled to 20-30 DEG C, seed tank culture liquid be added
It is 18-25 DEG C in temperature, mixing speed is cultivated 8-12 days under the conditions of being 160-200 revs/min, tank in fermentation process in fermentor
0.04-0.06MPa is pressed, the ventilatory capacity of filtrated air is (0.5-1) vvm, obtains fermentation tank culture liquid；
(4) be centrifuged: by fermentation tank culture liquid 3000-5000 revs/min centrifugation 20-30 minutes, abandoning supernatant, precipitated
Object；
(5) ultra low temperature vacuum is dry: in absolute pressure being 0.01-0.03MPa by sediment, temperature is 30-40 DEG C of drying
It to constant weight, crushes, crosses 60-100 mesh, obtain aweto mycelium.
The ventilatory capacity of the filtrated air is the volume of the interior filtrated air by unit volume culture medium per minute, such as:
The volume of culture medium is 3m in fermentor3, it is passed through 1.5m per minute3Filtrated air, then ventilation ratio be 3:1.5=1:0.5, lead to
Tolerance is 0.5vvm.
Provided by the present invention for improving the culture medium of aweto mycelium active constituent, aweto can be shortened
The cultivation cycle of filament significantly improves the content of Cordyceps sinensis polysaccharide in aweto mycelium, cordycepic acid, cordycepin and adenosine, tool
There is high nutritive value.
Specific embodiment
In the present invention, if not refering in particular to, all devices and raw material is commercially available or the industry is common are following
Method in embodiment is unless otherwise instructed conventional method in that art.
Alpha-amylase, the emerging Biotechnology Co., Ltd in Wuhan one hundred provide, 50,000 U/g of enzyme activity.
Cellulase, the emerging Biotechnology Co., Ltd in Wuhan one hundred provide, 50,000 U/g of enzyme activity.
Acid protease, the Nanning road Dong Henghua Biotechnology Co., Ltd provide, 50,000 U/g of enzyme activity
Medical stone, the medical stone provided using Wen County Hong Yu active carbon factory, 100 mesh of partial size.
Kuh-seng, the dry root of leguminous plant kuh-seng Sophora flavescens Ait..Bozhou Bai Chuan pharmaceutcal corporation, Ltd
It provides, the place of production: Jilin.
Glucose, the food-grade glucose provided using the satisfactory Biotechnology Co., Ltd in Hunan ten thousand.
Peptone, Wei Feng Biotechnology Co., Ltd of Zhengzhou City provide.
Potassium dihydrogen phosphate, Langfang Peng Cai Fine Chemical Co., Ltd provide, pharmaceutical grade.
Magnesium sulfate, the epsom salt that Langfang Peng Cai Fine Chemical Co., Ltd provides, pharmaceutical grade.
Hericium erinaceus, Mycophyta Basidiomycetes Aphyllophorales tooth bacterium section Hericium erinaceus Hericium erinaceus (Bull.ex
Fr.) the fructification of Pers..Xichou County Xiang Guang medicinal material Co., Ltd provides, the place of production: Yunnan.
Egg albumen powder, the egg albumen powder that the emerging Bioisystech Co., Ltd in Wuhan one hundred provides.
Hirsutella hepiali Chen et Shen kind is bought from Chinese industrial Culture Collection, bacterium numbering: 14017.
Crusher, the fruit crusher produced using Jingjiang City spy prestige mechanical equipment factory, model PS, particle size after cracking 5-
8mm。
Cordycepin and Determination of Adenosine: referring to Guo Tao, dragon etc. " different culture medium cultivate cordycepin in Cordyceps militaris and
Determination of Adenosine " it is tested.
Cordyceps sinensis polysaccharide and cordycepic acid content measurement: referring to Zhu Mingwei, warm Shandong etc., " different culture solutions are to Polysaccharides in Cultured Cordyceps militaris and worm
The influence of oxalic acid content " it is tested.
Embodiment 1
A kind of preparation method of aweto mycelium, comprising the following steps:
(1) 2000mL conical flask loading 1000mL inoculated and cultured: is used to improve aweto mycelium active constituent
Culture medium sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, Hirsutella hepiali Chen et Shen kind is aseptically inoculated with, in 20 DEG C of perseverances
Culture to mycelial concentration is 32wt% on warm shaking table, and shaking speed is 160 revs/min, obtains conical flask strain cultivation liquid；
(2) culture for improving aweto mycelium active constituent seed tank culture: is packed into 200L fermentor
Base 100L sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, conical flask strain cultivation liquid is added in fermentor, in temperature
It is 20 DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa, the ventilation of filtrated air in fermentation process
Amount is 0.6vvm, obtains seed tank culture liquid；
(3) training for improving aweto mycelium active constituent fermentation tank culture: is packed into the fermentor of 2000L
Base 1200L is supported, sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, seed tank culture liquid is added in fermentor, is 20 in temperature
DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa in fermentation process, and the ventilatory capacity of filtrated air is
0.6vvm obtains fermentation tank culture liquid；
(4) it is centrifuged: fermentation tank culture liquid being centrifuged 30 minutes at 4000 revs/min, supernatant is abandoned, obtains sediment；
(5) ultra low temperature vacuum is dry: in absolute pressure being 0.02MPa by sediment, temperature is 35 DEG C dry to constant weight, powder
It is broken, 80 meshes are crossed, aweto mycelium is obtained.
The culture medium for improving aweto mycelium active constituent is mixed to get by the raw material of following weight parts:
6 parts of potato juice, 4 parts of nutrient powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of potassium dihydrogen phosphate, 0.04 part of magnesium sulfate, wheat
150 parts of meal stone water.
The nutrient powder is egg albumen powder.
The potato juice is prepared by the following method to obtain, and described part is parts by weight: by peeling potatoes, bud eye is removed,
With potato residues are obtained after broken crusher machine, 10 parts of potato residues and 40 parts of water are mixed, 50 minutes is kept the temperature at 95 DEG C, uses
300 mesh filter-cloth filterings, obtain potato juice.
The Chinese medical stone water is prepared by the following method to obtain, and described part is parts by weight: 1 part of medical stone and 30 parts of water are mixed
It closes, stands 25 minutes at 25 DEG C, be then sonicated 15 minutes, using 800 mesh filter-cloth filterings, filtrate is Chinese medical stone water, described super
The condition of sonication are as follows: 50 DEG C of ultrasonic temperature, supersonic frequency 30KHz, ultrasonic power 120W.
Comparative example 1
A kind of preparation method of aweto mycelium, comprising the following steps:
(1) 2000mL conical flask loading 1000mL inoculated and cultured: is used to improve aweto mycelium active constituent
Culture medium sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, Hirsutella hepiali Chen et Shen kind is aseptically inoculated with, in 20 DEG C of perseverances
Culture to mycelial concentration is 32wt% on warm shaking table, and shaking speed is 160 revs/min, obtains conical flask strain cultivation liquid；
(2) culture for improving aweto mycelium active constituent seed tank culture: is packed into 200L fermentor
Base 100L sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, conical flask strain cultivation liquid is added in fermentor, in temperature
It is 20 DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa, the ventilation of filtrated air in fermentation process
Amount is 0.6vvm, obtains seed tank culture liquid；
(3) training for improving aweto mycelium active constituent fermentation tank culture: is packed into the fermentor of 2000L
Base 1200L is supported, sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, seed tank culture liquid is added in fermentor, is 20 in temperature
DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa in fermentation process, and the ventilatory capacity of filtrated air is
0.6vvm obtains fermentation tank culture liquid；
(4) it is centrifuged: fermentation tank culture liquid being centrifuged 30 minutes at 4000 revs/min, supernatant is abandoned, obtains sediment；
(5) ultra low temperature vacuum is dry: in absolute pressure being 0.02MPa by sediment, temperature is 35 DEG C dry to constant weight, powder
It is broken, 80 meshes are crossed, aweto mycelium is obtained.
The culture medium for improving aweto mycelium active constituent is mixed by the raw material of following weight parts
Arrive: 6 parts of potato juice, 4 parts of nutrient powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of potassium dihydrogen phosphate, 0.04 part of magnesium sulfate,
150 parts of water.
The nutrient powder is egg albumen powder.
The potato juice is prepared by the following method to obtain, and described part is parts by weight: by peeling potatoes, bud eye is removed,
With potato residues are obtained after broken crusher machine, 10 parts of potato residues and 40 parts of water are mixed, 50 minutes is kept the temperature at 95 DEG C, uses
300 mesh filter-cloth filterings, obtain potato juice.
Embodiment 2
A kind of preparation method of aweto mycelium, comprising the following steps:
(1) 2000mL conical flask loading 1000mL inoculated and cultured: is used to improve aweto mycelium active constituent
Culture medium sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, Hirsutella hepiali Chen et Shen kind is aseptically inoculated with, in 20 DEG C of perseverances
Culture to mycelial concentration is 32wt% on warm shaking table, and shaking speed is 160 revs/min, obtains conical flask strain cultivation liquid；
(2) culture for improving aweto mycelium active constituent seed tank culture: is packed into 200L fermentor
Base 100L sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, conical flask strain cultivation liquid is added in fermentor, in temperature
It is 20 DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa, the ventilation of filtrated air in fermentation process
Amount is 0.6vvm, obtains seed tank culture liquid；
(3) training for improving aweto mycelium active constituent fermentation tank culture: is packed into the fermentor of 2000L
Base 1200L is supported, sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, seed tank culture liquid is added in fermentor, is 20 in temperature
DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa in fermentation process, and the ventilatory capacity of filtrated air is
0.6vvm obtains fermentation tank culture liquid；
(4) it is centrifuged: fermentation tank culture liquid being centrifuged 30 minutes at 4000 revs/min, supernatant is abandoned, obtains sediment；
(5) ultra low temperature vacuum is dry: in absolute pressure being 0.02MPa by sediment, temperature is 35 DEG C dry to constant weight, powder
It is broken, 80 meshes are crossed, aweto mycelium is obtained.
The culture medium for improving aweto mycelium active constituent is mixed by the raw material of following weight parts
Arrive: 6 parts of potato juice, 4 parts of nutrient powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of potassium dihydrogen phosphate, 0.04 part of magnesium sulfate,
0.6 part of Radix Sophorae Flavescentis extractive liquid, 150 parts of Chinese medical stone water.
The nutrient powder is egg albumen powder.
The preparation method is the same as that of Example 1 for the potato juice.
The preparation method is the same as that of Example 1 for the Chinese medical stone water.
The preparation method of the Radix Sophorae Flavescentis extractive liquid includes the following steps that described part is parts by weight:
(1) kuh-seng is crushed, crosses 40 meshes, obtains kuh-seng powder, 8 parts of kuh-seng powder and 80 parts of water are mixed, are added 0.05 part
Mixed enzyme, the aqueous citric acid solution for being 10% with mass fraction adjust pH be 6.0,45 DEG C with 200 revs/min stir 2 hours,
Obtain enzymolysis liquid；
(2) enzymolysis liquid 90 DEG C refluxing extraction 80 minutes, using 300 mesh filter-cloth filterings, obtain filtrate and filter residue, in filter residue plus
Enter 60 parts of water, 90 DEG C refluxing extraction 50 minutes, using 300 mesh filter-cloth filterings, merge filtrate twice, be in absolute pressure
0.02MPa, temperature are that 50 DEG C of density for being concentrated under reduced pressure into 50 DEG C are 1.10g/mL, obtain Radix Sophorae Flavescentis extractive liquid.
The mixed enzyme is the cellulase of 75wt% and the mixture of 25wt% alpha-amylase.
Embodiment 3
A kind of preparation method of aweto mycelium, comprising the following steps:
(1) 2000mL conical flask loading 1000mL inoculated and cultured: is used to improve aweto mycelium active constituent
Culture medium sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, Hirsutella hepiali Chen et Shen kind is aseptically inoculated with, in 20 DEG C of perseverances
Culture to mycelial concentration is 32wt% on warm shaking table, and shaking speed is 160 revs/min, obtains conical flask strain cultivation liquid；
(2) culture for improving aweto mycelium active constituent seed tank culture: is packed into 200L fermentor
Base 100L sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, conical flask strain cultivation liquid is added in fermentor, in temperature
It is 20 DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa, the ventilation of filtrated air in fermentation process
Amount is 0.6vvm, obtains seed tank culture liquid；
(3) training for improving aweto mycelium active constituent fermentation tank culture: is packed into the fermentor of 2000L
Base 1200L is supported, sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, seed tank culture liquid is added in fermentor, is 20 in temperature
DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa in fermentation process, and the ventilatory capacity of filtrated air is
0.6vvm obtains fermentation tank culture liquid；
(4) it is centrifuged: fermentation tank culture liquid being centrifuged 30 minutes at 4000 revs/min, supernatant is abandoned, obtains sediment；
(5) ultra low temperature vacuum is dry: in absolute pressure being 0.02MPa by sediment, temperature is 35 DEG C dry to constant weight, powder
It is broken, 80 meshes are crossed, aweto mycelium is obtained.
The culture medium for improving aweto mycelium active constituent is mixed by the raw material of following weight parts
Arrive: 6 parts of potato juice, 4 parts of nutrient powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of potassium dihydrogen phosphate, 0.04 part of magnesium sulfate,
0.6 part of Radix Sophorae Flavescentis extractive liquid, 150 parts of Chinese medical stone water.
The nutrient powder is egg albumen powder.
The preparation method is the same as that of Example 1 for the potato juice.
The preparation method is the same as that of Example 1 for the Chinese medical stone water.
The preparation method of the Radix Sophorae Flavescentis extractive liquid includes the following steps that described part is parts by weight: kuh-seng being crushed, crosses 40
Mesh obtains kuh-seng powder, and 8 parts of kuh-seng powder and 80 parts of water are mixed, 90 DEG C refluxing extraction 80 minutes, using 300 mesh filter cloth mistakes
Filter, obtains filtrate and filter residue, and 60 parts of water are added in filter residue, 90 DEG C refluxing extraction 50 minutes, using 300 mesh filter-cloth filterings, merge
Filtrate twice obtains hardship absolute pressure is 0.02MPa, temperature is that 50 DEG C of density for being concentrated under reduced pressure into 50 DEG C are 1.10g/mL
Join extracting solution.
Embodiment 4
A kind of preparation method of aweto mycelium, comprising the following steps:
(1) 2000mL conical flask loading 1000mL inoculated and cultured: is used to improve aweto mycelium active constituent
Culture medium sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, Hirsutella hepiali Chen et Shen kind is aseptically inoculated with, in 20 DEG C of perseverances
Culture to mycelial concentration is 32wt% on warm shaking table, and shaking speed is 160 revs/min, obtains conical flask strain cultivation liquid；
(2) culture for improving aweto mycelium active constituent seed tank culture: is packed into 200L fermentor
Base 100L sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, conical flask strain cultivation liquid is added in fermentor, in temperature
It is 20 DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa, the ventilation of filtrated air in fermentation process
Amount is 0.6vvm, obtains seed tank culture liquid；
(3) training for improving aweto mycelium active constituent fermentation tank culture: is packed into the fermentor of 2000L
Base 1200L is supported, sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, seed tank culture liquid is added in fermentor, is 20 in temperature
DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa in fermentation process, and the ventilatory capacity of filtrated air is
0.6vvm obtains fermentation tank culture liquid；
(4) it is centrifuged: fermentation tank culture liquid being centrifuged 30 minutes at 4000 revs/min, supernatant is abandoned, obtains sediment；
(5) ultra low temperature vacuum is dry: in absolute pressure being 0.02MPa by sediment, temperature is 35 DEG C dry to constant weight, powder
It is broken, 80 meshes are crossed, aweto mycelium is obtained.
The culture medium for improving aweto mycelium active constituent is mixed by the raw material of following weight parts
Arrive: 6 parts of potato juice, 4 parts of nutrient powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of potassium dihydrogen phosphate, 0.04 part of magnesium sulfate,
0.6 part of Radix Sophorae Flavescentis extractive liquid, 150 parts of Chinese medical stone water.
The nutrient powder is egg albumen powder.
The preparation method is the same as that of Example 1 for the potato juice.
The preparation method is the same as that of Example 1 for the Chinese medical stone water.
The preparation method of the Radix Sophorae Flavescentis extractive liquid includes the following steps that described part is parts by weight:
(1) kuh-seng is crushed, crosses 40 meshes, obtains kuh-seng powder, 8 parts of kuh-seng powder and 80 parts of water are mixed, are added 0.05 part
Cellulase, it is 6.0 that the aqueous citric acid solution for being 10% with mass fraction, which adjusts pH, 2 small with 200 revs/min of stirrings at 45 DEG C
When, obtain enzymolysis liquid；
(2) enzymolysis liquid 90 DEG C refluxing extraction 80 minutes, using 300 mesh filter-cloth filterings, obtain filtrate and filter residue, in filter residue plus
Enter 60 parts of water, 90 DEG C refluxing extraction 50 minutes, using 300 mesh filter-cloth filterings, merge filtrate twice, be in absolute pressure
0.02MPa, temperature are that 50 DEG C of density for being concentrated under reduced pressure into 50 DEG C are 1.10g/mL, obtain Radix Sophorae Flavescentis extractive liquid.
Embodiment 5
A kind of preparation method of aweto mycelium, comprising the following steps:
(1) 2000mL conical flask loading 1000mL inoculated and cultured: is used to improve aweto mycelium active constituent
Culture medium sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, Hirsutella hepiali Chen et Shen kind is aseptically inoculated with, in 20 DEG C of perseverances
Culture to mycelial concentration is 32wt% on warm shaking table, and shaking speed is 160 revs/min, obtains conical flask strain cultivation liquid；
(2) culture for improving aweto mycelium active constituent seed tank culture: is packed into 200L fermentor
Base 100L sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, conical flask strain cultivation liquid is added in fermentor, in temperature
It is 20 DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa, the ventilation of filtrated air in fermentation process
Amount is 0.6vvm, obtains seed tank culture liquid；
(3) training for improving aweto mycelium active constituent fermentation tank culture: is packed into the fermentor of 2000L
Base 1200L is supported, sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, seed tank culture liquid is added in fermentor, is 20 in temperature
DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa in fermentation process, and the ventilatory capacity of filtrated air is
0.6vvm obtains fermentation tank culture liquid；
(4) it is centrifuged: fermentation tank culture liquid being centrifuged 30 minutes at 4000 revs/min, supernatant is abandoned, obtains sediment；
(5) ultra low temperature vacuum is dry: in absolute pressure being 0.02MPa by sediment, temperature is 35 DEG C dry to constant weight, powder
It is broken, 80 meshes are crossed, aweto mycelium is obtained.
The culture medium for improving aweto mycelium active constituent is mixed by the raw material of following weight parts
Arrive: 6 parts of potato juice, 4 parts of nutrient powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of potassium dihydrogen phosphate, 0.04 part of magnesium sulfate,
0.6 part of Radix Sophorae Flavescentis extractive liquid, 150 parts of Chinese medical stone water.
The nutrient powder is egg albumen powder.
The preparation method is the same as that of Example 1 for the potato juice.
The preparation method is the same as that of Example 1 for the Chinese medical stone water.
The preparation method of the Radix Sophorae Flavescentis extractive liquid includes the following steps that described part is parts by weight:
(1) kuh-seng is crushed, crosses 40 meshes, obtains kuh-seng powder, 8 parts of kuh-seng powder and 80 parts of water are mixed, are added 0.05 part
Alpha-amylase, it is 6.0 that the aqueous citric acid solution for being 10% with mass fraction, which adjusts pH, 2 small with 200 revs/min of stirrings at 45 DEG C
When, obtain enzymolysis liquid；
(2) enzymolysis liquid 90 DEG C refluxing extraction 80 minutes, using 300 mesh filter-cloth filterings, obtain filtrate and filter residue, in filter residue plus
Enter 60 parts of water, 90 DEG C refluxing extraction 50 minutes, using 300 mesh filter-cloth filterings, merge filtrate twice, be in absolute pressure
0.02MPa, temperature are that 50 DEG C of density for being concentrated under reduced pressure into 50 DEG C are 1.10g/mL, obtain Radix Sophorae Flavescentis extractive liquid.
Embodiment 6
A kind of preparation method of aweto mycelium, comprising the following steps:
(1) 2000mL conical flask loading 1000mL inoculated and cultured: is used to improve aweto mycelium active constituent
Culture medium sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, Hirsutella hepiali Chen et Shen kind is aseptically inoculated with, in 20 DEG C of perseverances
Culture to mycelial concentration is 32wt% on warm shaking table, and shaking speed is 160 revs/min, obtains conical flask strain cultivation liquid；
(2) culture for improving aweto mycelium active constituent seed tank culture: is packed into 200L fermentor
Base 100L sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, conical flask strain cultivation liquid is added in fermentor, in temperature
It is 20 DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa, the ventilation of filtrated air in fermentation process
Amount is 0.6vvm, obtains seed tank culture liquid；
(3) training for improving aweto mycelium active constituent fermentation tank culture: is packed into the fermentor of 2000L
Base 1200L is supported, sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, seed tank culture liquid is added in fermentor, is 20 in temperature
DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa in fermentation process, and the ventilatory capacity of filtrated air is
0.6vvm obtains fermentation tank culture liquid；
(4) it is centrifuged: fermentation tank culture liquid being centrifuged 30 minutes at 4000 revs/min, supernatant is abandoned, obtains sediment；
(5) ultra low temperature vacuum is dry: in absolute pressure being 0.02MPa by sediment, temperature is 35 DEG C dry to constant weight, powder
It is broken, 80 meshes are crossed, aweto mycelium is obtained.
The culture medium for improving aweto mycelium active constituent is mixed by the raw material of following weight parts
Arrive: 6 parts of potato juice, 4 parts of nutrient powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of potassium dihydrogen phosphate, 0.04 part of magnesium sulfate,
0.6 part of Radix Sophorae Flavescentis extractive liquid, 150 parts of Chinese medical stone water.
The nutrient powder is Hericium erinaceus extract.
The Hericium erinaceus extract is prepared by the following method to obtain, and described part is parts by weight: Hericium erinaceus is dry at 50 DEG C
Dry to constant weight, crushing crosses 80 meshes, obtains hedgehog hydnum mushroom powder, and 8 parts of hedgehog hydnum mushroom powders and 120 parts of water are mixed, are stirred with 200 revs/min
8 minutes, 0.04 part of acid protease and 0.06 part of cellulase are added, the salt acid for adjusting pH for being 10% with mass fraction is
4.0, then stirred 2 hours at 40 DEG C with 200 revs/min, enzymolysis liquid is obtained, enzymolysis liquid is kept the temperature 6 minutes at 95 DEG C, then with 4000
Revs/min centrifugation 20 minutes, supernatant was absolute pressure is 0.02MPa, temperature is that 40 DEG C of density for being concentrated under reduced pressure into 40 DEG C are
1.10g/mL, then be spray-dried, obtain Hericium erinaceus extract, the condition of the spray drying are as follows: inlet air temperature is 170 DEG C, out
Air temperature is 90 DEG C.
The potato juice is prepared by the following method to obtain, and described part is parts by weight: by peeling potatoes, bud eye is removed,
With potato residues are obtained after broken crusher machine, 10 parts of potato residues and 40 parts of water are mixed, 50 minutes is kept the temperature at 95 DEG C, uses
300 mesh filter-cloth filterings, obtain potato juice.
The Chinese medical stone water is prepared by the following method to obtain, and described part is parts by weight: 1 part of medical stone and 30 parts of water are mixed
It closes, stands 25 minutes at 25 DEG C, be then sonicated 15 minutes, using 800 mesh filter-cloth filterings, filtrate is Chinese medical stone water, described super
The condition of sonication are as follows: 50 DEG C of ultrasonic temperature, supersonic frequency 30KHz, ultrasonic power 120W.
The preparation method of the Radix Sophorae Flavescentis extractive liquid includes the following steps that described part is parts by weight:
(1) kuh-seng is crushed, crosses 40 meshes, obtains kuh-seng powder, 8 parts of kuh-seng powder and 80 parts of water are mixed, are added 0.05 part
Mixed enzyme, the aqueous citric acid solution for being 10% with mass fraction adjust pH be 6.0,45 DEG C with 200 revs/min stir 2 hours,
Obtain enzymolysis liquid；
(2) enzymolysis liquid 90 DEG C refluxing extraction 80 minutes, using 300 mesh filter-cloth filterings, obtain filtrate and filter residue, in filter residue plus
Enter 60 parts of water, 90 DEG C refluxing extraction 50 minutes, using 300 mesh filter-cloth filterings, merge filtrate twice, be in absolute pressure
0.02MPa, temperature are that 50 DEG C of density for being concentrated under reduced pressure into 50 DEG C are 1.10g/mL, obtain Radix Sophorae Flavescentis extractive liquid.
The mixed enzyme is the cellulase of 75wt% and the mixture of 25wt% alpha-amylase.
The active component content test result of obtained aweto mycelium: Cordyceps sinensis polysaccharide 65.35mg/g, cordycepic acid
163.21, cordycepin 7.36mg/g, adenosine 10.17mg/g.
Embodiment 7
A kind of preparation method of aweto mycelium, comprising the following steps:
(1) 2000mL conical flask loading 1000mL inoculated and cultured: is used to improve aweto mycelium active constituent
Culture medium sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, Hirsutella hepiali Chen et Shen kind is aseptically inoculated with, in 20 DEG C of perseverances
Culture to mycelial concentration is 32wt% on warm shaking table, and shaking speed is 160 revs/min, obtains conical flask strain cultivation liquid；
(2) culture for improving aweto mycelium active constituent seed tank culture: is packed into 200L fermentor
Base 100L sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, conical flask strain cultivation liquid is added in fermentor, in temperature
It is 20 DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa, the ventilation of filtrated air in fermentation process
Amount is 0.6vvm, obtains seed tank culture liquid；
(3) training for improving aweto mycelium active constituent fermentation tank culture: is packed into the fermentor of 2000L
Base 1200L is supported, sterilizes 30 minutes at 121 DEG C, is cooled to 25 DEG C, seed tank culture liquid is added in fermentor, is 20 in temperature
DEG C, mixing speed is cultivated 10 days under the conditions of being 180 revs/min, and tank presses 0.05MPa in fermentation process, and the ventilatory capacity of filtrated air is
0.6vvm obtains fermentation tank culture liquid；
(4) it is centrifuged: fermentation tank culture liquid being centrifuged 30 minutes at 4000 revs/min, supernatant is abandoned, obtains sediment；
(5) ultra low temperature vacuum is dry: in absolute pressure being 0.02MPa by sediment, temperature is 35 DEG C dry to constant weight, powder
It is broken, 80 meshes are crossed, aweto mycelium is obtained.
The culture medium for improving aweto mycelium active constituent is mixed by the raw material of following weight parts
Arrive: 6 parts of potato juice, 4 parts of nutrient powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of potassium dihydrogen phosphate, 0.04 part of magnesium sulfate,
0.6 part of Radix Sophorae Flavescentis extractive liquid, 150 parts of Chinese medical stone water.
The nutrient powder is the mixture of Hericium erinaceus extract and egg albumen powder, wherein the Hericium erinaceus extract and chicken
The mass ratio of egg albumen powder is 3:1.
The Hericium erinaceus extract is prepared by the following method to obtain, and described part is parts by weight: Hericium erinaceus is dry at 50 DEG C
Dry to constant weight, crushing crosses 80 meshes, obtains hedgehog hydnum mushroom powder, and 8 parts of hedgehog hydnum mushroom powders and 120 parts of water are mixed, are stirred with 200 revs/min
8 minutes, 0.04 part of acid protease and 0.06 part of cellulase are added, the salt acid for adjusting pH for being 10% with mass fraction is
4.0, then stirred 2 hours at 40 DEG C with 200 revs/min, enzymolysis liquid is obtained, enzymolysis liquid is kept the temperature 6 minutes at 95 DEG C, then with 4000
Revs/min centrifugation 20 minutes, supernatant was absolute pressure is 0.02MPa, temperature is that 40 DEG C of density for being concentrated under reduced pressure into 40 DEG C are
1.10g/mL, then be spray-dried, obtain Hericium erinaceus extract, the condition of the spray drying are as follows: inlet air temperature is 170 DEG C, out
Air temperature is 90 DEG C.
The potato juice is prepared by the following method to obtain, and described part is parts by weight: by peeling potatoes, bud eye is removed,
With potato residues are obtained after broken crusher machine, 10 parts of potato residues and 40 parts of water are mixed, 50 minutes is kept the temperature at 95 DEG C, uses
300 mesh filter-cloth filterings, obtain potato juice.
The Chinese medical stone water is prepared by the following method to obtain, and described part is parts by weight: 1 part of medical stone and 30 parts of water are mixed
It closes, stands 25 minutes at 25 DEG C, be then sonicated 15 minutes, using 800 mesh filter-cloth filterings, filtrate is Chinese medical stone water, described super
The condition of sonication are as follows: 50 DEG C of ultrasonic temperature, supersonic frequency 30KHz, ultrasonic power 120W.
The preparation method of the Radix Sophorae Flavescentis extractive liquid includes the following steps that described part is parts by weight:
(1) kuh-seng is crushed, crosses 40 meshes, obtains kuh-seng powder, 8 parts of kuh-seng powder and 80 parts of water are mixed, are added 0.05 part
Mixed enzyme, the aqueous citric acid solution for being 10% with mass fraction adjust pH be 6.0,45 DEG C with 200 revs/min stir 2 hours,
Obtain enzymolysis liquid；
(2) enzymolysis liquid 90 DEG C refluxing extraction 80 minutes, using 300 mesh filter-cloth filterings, obtain filtrate and filter residue, in filter residue plus
Enter 60 parts of water, 90 DEG C refluxing extraction 50 minutes, using 300 mesh filter-cloth filterings, merge filtrate twice, be in absolute pressure
0.02MPa, temperature are that 50 DEG C of density for being concentrated under reduced pressure into 50 DEG C are 1.10g/mL, obtain Radix Sophorae Flavescentis extractive liquid.
The mixed enzyme is the cellulase of 75wt% and the mixture of 25wt% alpha-amylase.
The active component content test result of obtained aweto mycelium: Cordyceps sinensis polysaccharide 79.45mg/g, cordycepic acid
186.64, cordycepin 8.41mg/g, adenosine 12.36mg/g.
Test case 1
Cordyceps sinensis polysaccharide and cordycepic acid content are tested in the aweto mycelium obtained to embodiment, specific test knot
Fruit is shown in Table 1.
1 aweto mycelium Cordyceps sinensis polysaccharide of table and cordycepic acid content test result table
Test case 2
Cordycepin and adenosine content are tested in the aweto mycelium obtained to embodiment, and specific test result is shown in
Table 2.
2 aweto mycelium cordycepin of table and adenosine content test result table
| Cordycepin, mg/g | Adenosine, mg/g | |
| Embodiment 1 | 6.25 | 8.27 |
| Comparative example 1 | 4.82 | 7.11 |
| Embodiment 2 | 7.28 | 9.96 |
| Embodiment 3 | 6.47 | 8.74 |
| Embodiment 4 | 6.73 | 9.01 |
| Embodiment 5 | 6.58 | 8.92 |
Embodiment 1 can significantly promote the growth of aweto mycelium containing various trace elements using Chinese medical stone water,
Improve the content of various beneficiating ingredients.It is added to shrubby sophora extract in embodiment 2, and the extracting method of shrubby sophora extract is carried out
Screening, preferably optimum extracting method out, obtained shrubby sophora extract can be obviously improved in aweto mycelium it is various beneficial at
The content divided.Comparing embodiment 2 and embodiment 3-5, discovery have preferable synergistic effect using amylase and cellulase.This
It may be because hindering the dissolution of effective component, the enzymatic hydrolysis of starch hinders the enzymatic hydrolysis of cellulose containing more starch in kuh-seng
Degree reduces, and improves enzymolysis efficiency.
Claims (9)
1. a kind of for improving the culture medium of aweto mycelium active constituent, which is characterized in that including following raw material: Ma Ling
Potato juice, nutrient powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, Chinese medical stone water.
2. as described in claim 1 for improving the culture medium of aweto mycelium active constituent, it is characterised in that: also wrap
Include Radix Sophorae Flavescentis extractive liquid.
3. as claimed in claim 2 for improving the culture medium of aweto mycelium active constituent, which is characterized in that under
The raw material for stating parts by weight is mixed to get: 4-8 parts of potato juice, 2-6 parts of nutrient powder, 1-3 parts of glucose, 0.1-0.5 parts of peptone,
0.02-0.08 parts of potassium dihydrogen phosphate, 0.02-0.06 parts of magnesium sulfate, 0.5-1 parts of Radix Sophorae Flavescentis extractive liquid, 120-180 parts of Chinese medical stone water.
4. the culture medium for being used to improve aweto mycelium active constituent as described in claims 1 or 2 or 3, feature exist
In the potato juice is prepared by the following method to obtain, and described part is parts by weight: by peeling potatoes, bud eye is removed, with broken
Obtain potato residues after crusher machine, 8-12 parts of potato residues and 30-50 parts of water mixed, 90-100 DEG C heat preservation 40-60 minutes,
Using 200-300 mesh filter-cloth filtering, potato juice is obtained.
5. the culture medium for being used to improve aweto mycelium active constituent as described in claims 1 or 2 or 3, feature exist
In the nutrient powder is Hericium erinaceus extract and/or egg albumen powder.
6. as claimed in claim 5 for improving the culture medium of aweto mycelium active constituent, it is characterised in that: described
Hericium erinaceus extract is prepared by the following method to obtain, and described part is parts by weight: Hericium erinaceus is dried at 40-60 DEG C to constant weight,
Crush, cross 60-100 mesh, obtain hedgehog hydnum mushroom powder, by 5-10 part hedgehog hydnum mushroom powders and the mixing of 100-150 part water, with 100-300 turns/
Divide stirring 5-10 minutes, adds 0.02-0.06 parts of acid proteases and 0.05-0.1 parts of cellulases, be with mass fraction
10% salt acid for adjusting pH be 3.5-4.5, then 35-45 DEG C with 100-300 revs/min stirring 1-3 hours, obtain enzymolysis liquid, general
Enzymolysis liquid 90-100 DEG C heat preservation 5-10 minutes, then with 3000-5000 revs/min centrifugation 10-20 minutes, supernatant be concentrated under reduced pressure
It is 1.08-1.12g/mL to 40 DEG C of density, then is spray-dried, obtains Hericium erinaceus extract.
7. the culture medium for being used to improve aweto mycelium active constituent as described in claims 1 or 2 or 3, feature exist
In the Chinese medical stone water is prepared by the following method to obtain, and described part is parts by weight: 1 part of medical stone and 20-40 parts of water are mixed
Close, 20-30 DEG C standing 20-30 minutes, be then sonicated 10-20 minutes, using 800 mesh filter-cloth filterings, filtrate is medical stone
Water；The condition of the ultrasonic treatment are as follows: 40-60 DEG C of ultrasonic temperature, supersonic frequency 25-35KHz, ultrasonic power 100-150W.
8. as claimed in claim 2 or claim 3 for improving the culture medium of aweto mycelium active constituent, which is characterized in that
The preparation method of the Radix Sophorae Flavescentis extractive liquid includes the following steps that described part is parts by weight:
(1) kuh-seng is crushed, crosses 20-50 mesh, obtains kuh-seng powder, 5-10 parts of kuh-seng powder and 60-100 parts of water are mixed, are added
It is 5.5-6.5 that aqueous citric acid solution that 0.01-0.1 parts of mixed enzyme is 10% with mass fraction, which adjusts pH, 40-50 DEG C with
100-300 revs/min stirring 1-3 hours, obtain enzymolysis liquid；
(2) enzymolysis liquid 85-95 DEG C refluxing extraction 60-90 minutes, using 300 mesh filter-cloth filterings, filtrate and filter residue are obtained, in filter residue
40-80 part water are added, 85-95 DEG C refluxing extraction 40-60 minute, using 300 mesh filter-cloth filterings, merging filtrate twice is depressurized
The density for being concentrated into 50 DEG C is 1.08-1.12g/mL, obtains Radix Sophorae Flavescentis extractive liquid.
9. as claimed in claim 8 for improving the culture medium of aweto mycelium active constituent, it is characterised in that: described
Mixed enzyme is the cellulase of 70-80wt% and the mixture of 20-30wt% alpha-amylase.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111728200A (en) * | 2020-07-03 | 2020-10-02 | 杭州雪域生物技术有限公司 | Cordyceps sinensis enzyme and preparation method and application thereof |
| CN112342145A (en) * | 2020-11-04 | 2021-02-09 | 青海久实虫草生物科技有限公司 | Rejuvenation method of hirsutella hepiali Chen et Shen fungi |
| CN114438153A (en) * | 2022-03-18 | 2022-05-06 | 浙江汇能生物股份有限公司 | Process for improving extraction rate of cordycepin from Guni cordyceps sinensis |
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| WO2000030475A1 (en) * | 1998-11-26 | 2000-06-02 | Wakamoto Pharmaceutical Co., Ltd. | Hair-nourishing foods |
| CN1362513A (en) * | 2001-01-04 | 2002-08-07 | 怀化市益康生物高科技有限公司 | Artificial cordyceps culturing medium |
| CN101177665A (en) * | 2006-11-06 | 2008-05-14 | 张刚 | Industrial method for culturing north winter worm summer herb with high-content of cordycepin |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000030475A1 (en) * | 1998-11-26 | 2000-06-02 | Wakamoto Pharmaceutical Co., Ltd. | Hair-nourishing foods |
| CN1362513A (en) * | 2001-01-04 | 2002-08-07 | 怀化市益康生物高科技有限公司 | Artificial cordyceps culturing medium |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111728200A (en) * | 2020-07-03 | 2020-10-02 | 杭州雪域生物技术有限公司 | Cordyceps sinensis enzyme and preparation method and application thereof |
| CN111728200B (en) * | 2020-07-03 | 2023-06-02 | 杭州雪域生物技术有限公司 | Cordyceps sinensis ferment as well as preparation method and application thereof |
| CN112342145A (en) * | 2020-11-04 | 2021-02-09 | 青海久实虫草生物科技有限公司 | Rejuvenation method of hirsutella hepiali Chen et Shen fungi |
| CN114438153A (en) * | 2022-03-18 | 2022-05-06 | 浙江汇能生物股份有限公司 | Process for improving extraction rate of cordycepin from Guni cordyceps sinensis |
| CN114438153B (en) * | 2022-03-18 | 2024-02-02 | 浙江汇能生物股份有限公司 | Process for improving extraction rate of cordycepin from Guni cordyceps sinensis |
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