CN109182103B - Automatic microorganism coating device - Google Patents

Automatic microorganism coating device Download PDF

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Publication number
CN109182103B
CN109182103B CN201811303391.9A CN201811303391A CN109182103B CN 109182103 B CN109182103 B CN 109182103B CN 201811303391 A CN201811303391 A CN 201811303391A CN 109182103 B CN109182103 B CN 109182103B
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coating rod
culture dish
coating
connecting block
mounting seat
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CN201811303391.9A
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CN109182103A (en
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陈中奇
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Chongqing Yingteli Technology Co ltd
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Chongqing Yingteli Technology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of microorganisms, in particular to an automatic microorganism coating device, which comprises a coating rod and a driving mechanism, coating rod mount pad and culture dish lid, the coating rod mount pad is located culture dish lid middle part and with culture dish lid sliding connection, culture dish lid up end is provided with ultraviolet lamp, still be provided with bacterial suspension dropwise add hole on the culture dish lid, be equipped with the cavity in the coating rod mount pad, coating rod mount pad bottom is equipped with the through-hole of intercommunication cavity and culture dish lid lower extreme terminal surface, the through-hole internal rotation is connected with the connecting block, the connecting block is driven rotatoryly by actuating mechanism, the connecting block can be dismantled with the coating rod and be connected, the coating rod is made by the gasbag, the perpendicular fixedly connected with stick head piece in coating rod middle part, be equipped with the air vent with the coating rod intercommunication on the stick head piece, actuating mechanism locates in the cavity of coating rod mount pad, be used for aerifing and drive the coating rod rotatory for the. The invention can reduce the probability of bacterial contamination of the culture dish in the coating operation process.

Description

Automatic microorganism coating device
Technical Field
The invention relates to the technical field of microorganisms, in particular to an automatic microorganism coating device.
Background
The coating inoculation is a common microorganism culture method, can be used for counting and separating microorganisms, and can also be used for observing the microorganisms by utilizing the characteristic that the microorganisms grow on the surface of a flat plate to form colonies. In the traditional microbial culture technology, the microbial coating operation needs to firstly carry out a series of gradient dilution on bacterial liquid, then bacterial liquids with different dilutions are respectively coated on the surface of an agar solid culture medium, and a culture dish and a coating rod are needed in the coating operation. When the culture medium is used specifically, an operator drops a proper amount of bacteria on the culture medium of a culture dish, and the bacteria liquid is coated back and forth by a coating rod to be coated on the surface of the culture medium, so that single bacterial colonies or bacterial lawn grow on the culture medium, and the purposes of separation, counting or culture observation are achieved.
When a culture dish is adopted for coating in the traditional microorganism coating method, the opening amplitude of the dish cover is large, the culture dish is exposed in the air for a long time in the coating operation process, and for a microorganism test with strict requirements on aseptic conditions, the probability that the culture medium is polluted by other bacteria can be increased due to the long-time large-scale contact of the culture medium and the air, so that the purposes of separating, counting or culturing and observing a single colony or a lawn are difficult to achieve.
Disclosure of Invention
The invention mainly solves the technical problem of providing an automatic microorganism coating device which can reduce the probability of bacterial contamination of a culture dish in the coating operation process, so that a single colony or lawn can achieve the purposes of separation, counting or culture observation.
In order to solve the technical problems, the invention provides an automatic microorganism coating device which comprises a coating rod, a driving mechanism, a coating rod installation seat and a culture dish cover body, wherein the coating rod installation seat is arranged in the middle of the culture dish cover body and is connected with the culture dish cover body in a vertical sliding manner, the culture dish cover body can rotate around the coating rod installation seat, an ultraviolet lamp is arranged on the upper end surface of the culture dish cover body, a bacterial suspension dropping hole is also formed in the culture dish cover body, a cover plate used for closing the bacterial suspension dropping hole is arranged on the culture dish cover body, a cavity is arranged in the coating rod installation seat, a through hole communicated with the cavity and the lower end surface of the coating rod installation seat is arranged at the bottom of the coating rod installation seat, a connecting block is rotationally connected in the through hole, the connecting block is driven to rotate by the driving mechanism and is detachably connected with the coating rod, the coating rod is made, the middle part of the coating rod is vertically and fixedly connected with a rod head block, the lower end face of the rod head block is provided with an accommodating groove for accommodating the coating rod, the rod head block is provided with a vent hole communicated with the coating rod, and the driving mechanism is arranged in a cavity of the coating rod mounting seat and is used for inflating and deflating the coating rod and driving the coating rod to rotate;
the drive assembly comprises a speed regulating motor, a hollow gear and a hollow rotating shaft, the speed regulating motor is fixed in a cavity of the coating rod mounting seat, a transmission gear is connected to the rotating shaft of the speed regulating motor, a bearing is fixedly connected to the inside of the through hole, the hollow rotating shaft is rotatably connected with the bearing, the hollow rotating shaft is fixedly connected with a connecting block, blades are fixedly connected to the inner side wall of the hollow rotating shaft, the hollow gear is fixedly connected to the hollow rotating shaft and meshed with the transmission gear, and a ventilation opening communicated with the cavity is formed in the outer surface of the coating rod mounting seat.
The working principle and the advantages of the invention are as follows:
1. the ultraviolet lamp is arranged on the upper end face of the culture dish cover body, so that a relatively closed space is formed between the culture dish cover body and the culture dish when the automatic microorganism coating device coats, external bacteria can be prevented from entering the culture dish, meanwhile, the ultraviolet lamp can sterilize the interior of the culture dish, a relatively sterile environment can be formed in the interior space of the culture dish, and the success rate of experiments can be improved; the arrangement of the bacterial suspension dripping holes on the culture dish cover body can ensure that when the bacterial suspension is dripped into the culture dish, the bacterial suspension can be dripped into the culture dish only by opening an opening smaller than the opening of the culture dish without opening the whole culture dish cover body, thereby reducing the probability of external bacteria entering the inner space of the culture dish and reducing the influence of the external bacteria on a microorganism culture experiment; the holding tank that is used for holding the coating rod that terminal surface was equipped with under the stick head piece can make the coating rod made by the gasbag in the holding tank of terminal surface under the stick head piece is taken in under the compression state, after aerifing for the coating rod by drive assembly, available ultraviolet lamp disinfects culture dish inner space, it is rotatory to drive the coating rod by drive assembly again after bacterial suspension dropwise add, evenly coat bacterial suspension, bacterial suspension's dropwise add to the contact probability that has reduced the inside and foreign object bacterium of culture dish of the whole in-process of coating to a great extent.
2. The connecting block can be dismantled with the coating rod and be connected, after the even coating of the bacterial suspension in a culture dish, when needing to carry out even coating to the bacterial suspension in other culture dishes, deflate the coating rod through drive assembly, during the holding tank of terminal surface under the coating rod retraction stick head piece after will using, only need after the recovery take off stick head piece change new can, the convenient recovery of concentrating of stick head piece that takes off simultaneously, cross contamination between the different culture dishes has also been avoided.
3. The coating rod mounting seat is arranged in the middle of the culture dish cover body, so that the culture dish cover body can rotate around the coating rod mounting seat, the ultraviolet lamp fixed on the culture dish cover body can uniformly sterilize the culture dish conveniently, the sterilization range of the ultraviolet lamp is enlarged, and the effect is better; because the demand of microorganism culture is different, and need use the culture dish of different specifications, lead to the top of culture dish lateral wall and the culture medium surface in the culture dish high diverse easily, coating rod and the high fixed coating device of culture dish lid, there may exist the coating rod and can not contact culture medium surface in the culture dish, it is difficult to carry out the coating operation to the culture dish of different specifications, and, sliding connection's design can let the culture dish lid reciprocate at the coating rod mount pad, can satisfy coating device and can both carry out the coating operation to the culture dish of different specifications, application scope is wider.
Before the bacterial suspension is coated, the speed regulating motor is started to rotate at a high speed to drive the transmission gear to rotate, the transmission gear drives the hollow gear meshed with the transmission gear, the hollow gear drives the hollow rotating shaft to rotate, the hollow gear drives the blades fixed with the hollow gear to rotate when rotating, the hollow rotating shaft forms air flow, the rotating direction of the hollow rotating shaft is controlled to control the flowing direction of the air flow, and the functions of inflating and expanding, rotary coating, air suction and recovery and the like of a coating rod can be realized.
Furthermore, limiting rings are respectively arranged on the side wall and the bottom of the coating rod mounting seat, and the culture dish cover body is located between the two limiting rings.
The design of spacing ring is in order to prevent that culture dish lid and coating rod mount pad from breaking away from, causes awkward problem.
Furthermore, a bacterial suspension dropping pipe is sleeved in the bacterial suspension dropping hole. The bacterium suspension dropwise add pipe is established downthehole at the bacterium suspension dropwise add, avoids bacterium suspension and the direct contact of culture dish lid to cause the culture dish lid to be polluted, produces cross contamination when leading to the culture dish lid to contact with other culture dishes, influences the microbial cultivation experiment effect.
Further, the connecting block is cylindrical, magnet has been inlayed to the connecting block inside, the lower terminal surface of connecting block is equipped with the recess that agrees with the club head piece, the club head piece has magnetism, the recess top surface is equipped with air inlet and the gas outlet of intercommunication connecting block up end, all be equipped with the check valve on air inlet and the gas outlet, be equipped with on the gas outlet and satisfy the threshold value and the mechanism that overflows that starts for prevent that the coating rod from being aerifyd excessively and giving the coating rod gassing and retrieving.
When the drive mechanism inflates the coating rod, the hollow rotating shaft rotates to enable gas generated by rotation of the blades to enter the coating rod through the gas inlet of the connecting block to inflate the coating rod, when the flushed gas is excessive, the pressure in the coating rod is increased, and when the pressure is increased to a certain threshold value, the overflow mechanism is started to discharge the excessive gas in the coating rod from the gas outlet, so that the coating rod is always kept in an inflated state, and the excessive inflation cannot cause explosion; when the driving mechanism deflates the coating rod, the hollow rotating shaft rotates to rotate the blades, so that negative pressure is generated between the blades and the overflow mechanism, the negative pressure reaches a certain threshold value, the overflow mechanism is started to discharge all gas in the coating rod body from the gas outlet, and meanwhile, the coating rod is recovered into the rod head block.
Further, overflow mechanism and include reset spring and baffle, the connecting block up end is equipped with the spacing groove, the spacing tank bottom is located to the gas outlet, baffle sliding connection covers on the gas outlet at the spacing inslot, be equipped with the exhaust hole of staggering the setting with the gas outlet on the baffle, reset spring connects respectively between connecting block and baffle for make the baffle closely cover close on the gas outlet.
The reset spring and the gravity action of the baffle plate enable the tight baffle plate to cover the air outlet, only when the pressure in the coating rod body is too high and reaches a certain threshold value or when the negative pressure above the baffle plate reaches a certain threshold value, the reset spring is stretched, meanwhile, the baffle plate slides upwards in the limiting groove, the air outlet is opened, the air can be discharged through the exhaust hole in the baffle plate, and the functions of automatic coating rod deflation recovery and prevention of explosion caused by excessive inflation of the coating rod can be realized by combining with the speed regulating motor.
Drawings
FIG. 1 is a partial cross-sectional view of an embodiment of an automated microbial application apparatus of the present invention;
FIG. 2 is an enlarged schematic view of the connector block of FIG. 1;
fig. 3 is a top view of the hollow shaft of fig. 1 connected to a transmission gear.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the device comprises a coating rod 1, a culture dish cover body 2, a limiting ring 3, a cover plate 4, a bacterial suspension dropping pipe 5, a speed regulating motor 6, a transmission gear 7, a coating rod mounting seat 8, a hollow gear 9, a hollow rotating shaft 10, a ventilation opening 11, a bearing 12, a connecting block 13, an ultraviolet lamp 14, a rod head block 15, a magnet 16, a one-way valve 17, an air inlet 18, a limiting groove 19, a baffle plate 20, an exhaust hole 21, a reset spring 22, an air outlet 23 and blades 24.
The embodiment is basically as shown in the attached figure 1:
an automatic microorganism coating device comprises a coating rod 1, a driving mechanism, a coating rod mounting seat 8 and a culture dish cover body 2, a circular through hole is arranged in the middle of the culture dish cover body 2, the coating rod mounting seat 8 is cylindrical, the coating rod mounting seat 8 is arranged in the circular through hole of the culture dish cover body 2 and is connected with the culture dish cover body 2 in a vertical sliding way, the culture dish cover body 2 can rotate around the coating rod mounting seat 8, the side wall and the bottom of the coating rod mounting seat 8 are respectively provided with a limiting ring 3, the culture dish cover body 2 is positioned between the two limit rings 3, the upper end surface of the culture dish cover body 2 is fixedly provided with an ultraviolet lamp 14, the culture dish cover body 2 is also provided with a bacterial suspension dropping hole, a bacterial suspension dropping pipe 5 is sleeved in the bacterial suspension dropping hole, and the culture dish cover body 2 is provided with a cover plate 4 for closing the bacterial suspension dropping hole;
a cavity is arranged in the coating rod mounting seat 8, a through hole for communicating the cavity with the lower end face of the coating rod mounting seat 8 is arranged at the bottom of the coating rod mounting seat 8, a connecting block 13 is rotationally connected in the through hole, the connecting block 13 is driven by a driving mechanism to rotate, the connecting block 13 is detachably connected with the coating rod 1, the coating rod 1 is made of an air bag, a rod head block 15 is vertically and fixedly connected to the middle of the coating rod 1, an accommodating groove for accommodating the coating rod 1 is arranged on the lower end face of the rod head block 15, and an air hole communicated with the coating rod 1 is formed in the rod head block 15;
the coating rod driving mechanism is arranged in a cavity of the coating rod mounting seat 8 and used for inflating and deflating the coating rod 1 and driving the coating rod 1 to rotate, the driving assembly comprises an adjustable speed motor 6, a hollow gear 9 and a hollow rotating shaft 10, the adjustable speed motor 6 is fixed in the cavity of the coating rod mounting seat 8, a transmission gear 7 is connected to a rotating shaft of the adjustable speed motor 6, a bearing 12 is fixedly connected to the inside of the through hole, as shown in fig. 3, the hollow rotating shaft 10 is rotatably connected with the bearing 12, the hollow rotating shaft 10 is fixedly connected with a connecting block 13, blades 24 are fixedly connected to the inner side wall of the hollow rotating shaft 10, the hollow gear 9 is fixedly connected to the hollow rotating shaft 10 and meshed with the transmission gear 7, and a ventilation opening 11 communicated with the cavity is formed in the outer.
The coating rod mounting structure is characterized in that the connecting block 13 is cylindrical, as shown in fig. 2, a magnet 16 is embedded in the connecting block 13, a groove matched with the rod head block 15 is formed in the lower end face of the connecting block 13, the rod head block 15 is not in contact with the coating rod mounting seat 8, the rod head block 15 is made of a magnetic iron block, an air inlet 18 and an air outlet 23 which are communicated with the upper end face of the connecting block 13 are formed in the top face of the groove, one-way valves 17 are arranged on the air inlet 18 and the air outlet 23, and an overflow mechanism which is started when a threshold value is met is arranged on the air outlet 23 and is used for preventing the coating rod 1 from being over-inflated and deflating.
Spill over the mechanism and include reset spring 22 and baffle 20, connecting block 13 up end is equipped with spacing groove 19, 19 bottoms in spacing groove are located to gas outlet 23, baffle 20 sliding connection closes on gas outlet 23 at spacing inslot 19 and lid, be equipped with the exhaust hole 21 that staggers the setting with gas outlet 23 on the baffle 20, 19 bottoms in spacing groove still are equipped with the spring standing groove for place reset spring 22, reset spring 22 connects respectively between connecting block 13 and baffle 20 for make baffle 20 closely cover close on gas outlet 23, whole opening and the hollow rotating shaft 10 intercommunication of spacing groove 19.
The specific implementation process is as follows:
firstly, starting the speed regulating motor 6 to rotate in the forward direction, regulating to a high-speed rotation mode, enabling the transmission gear 7 to rotate at a high speed, driving the hollow rotating shaft 10 to rotate at a high speed through the hollow gear 9, further enabling the blades 24 to rotate at a high speed, generating downward flowing gas in the hollow rotating shaft 10, enabling the gas to enter the rod head block 15 through the connecting block 13, inflating the coating rod 1 contracted in the accommodating groove on the lower end face of the rod head block 15, enabling the coating rod 1 to be fully expanded, and stopping the speed regulating motor 6; when too much gas is flushed, the pressure in the coating rod 1 is increased, when the pressure is increased to a certain threshold value, the overflow mechanism is started, so that the baffle plate 20 of the limiting groove 19 is jacked upwards, the exhaust hole 21 on the baffle plate 20 is communicated with the air inlet 18, the redundant gas in the coating rod 1 is exhausted from the air outlet 23, when the coating rod 1 is restored to a certain pressure, the reset spring 22 drives the baffle plate 20 to move downwards, the air outlet 23 is covered, the coating rod 1 is always kept in a filling state, and the phenomenon that the explosion is generated due to over-inflation is avoided;
then cover 2 with the culture dish lid on the culture dish, start ultraviolet lamp 14, rotate culture dish lid 2 and make ultraviolet lamp 14 carry out germicidal treatment to whole culture dish, open the apron 4 on the bacterial suspension dropwise add hole on the culture dish lid 2 again, pack bacterial suspension dropwise add pipe 5 into in the bacterial suspension dropwise add hole, avoid bacterial suspension and culture dish lid 2 direct contact to cause the pollution, lead to whole device and other culture dishes to produce cross contamination, influence microbial cultivation experiment effect.
Then starting the speed regulating motor 6, regulating to a low-speed rotation mode, and driving the hollow rotating shaft 10 to rotate at a low speed, so that the coating rod 1 is driven to rotate at a low speed through the connecting block 13, and the bacterial suspension is uniformly coated; after the coating is finished, the speed regulating motor 6 is stopped, and the culture dish cover body 2 is taken down from the culture dish.
When a new coating rod 1 needs to be replaced, the speed regulating motor 6 is reversely started, the high-speed rotation mode is adjusted, the transmission gear 7 rotates at a high speed, the hollow gear 9 drives the hollow rotating shaft 10 to rotate at a high speed, the blades 24 rotate at a high speed, the hollow rotating shaft 10 rotates to enable the blades 24 to rotate, negative pressure is generated between the blades 24 and the overflow mechanism, due to the arrangement of the one-way valve 17, gas in the coating rod 1 can only flow out from the gas outlet 23 through the overflow mechanism, when the negative pressure reaches a certain threshold value, the baffle 20 in the limiting groove 19 is driven to move upwards, the exhaust holes 21 in the baffle 20 are communicated with the gas inlet 18, all gas in the coating rod 1 is exhausted from the gas outlet 23, finally, the coating rod 1 is recovered into the accommodating groove of the rod head block 15, and the used coating rod 1 is taken down and replaced with a new coating rod.
The foregoing is merely an example of the present invention, and common general knowledge in the field of known specific structures and characteristics is not described herein in any greater extent than that known in the art at the filing date or prior to the priority date of the application, so that those skilled in the art can now appreciate that all of the above-described techniques in this field and have the ability to apply routine experimentation before this date can be combined with one or more of the present teachings to complete and implement the present invention, and that certain typical known structures or known methods do not pose any impediments to the implementation of the present invention by those skilled in the art. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims (5)

1. Automatic microorganism coating unit, including coating rod, actuating mechanism, coating rod mount pad and culture dish lid, its characterized in that: the coating rod mounting seat is arranged in the middle of the culture dish cover body and is connected with the culture dish cover body in a vertical sliding mode, the culture dish cover body can rotate around the coating rod mounting seat, an ultraviolet lamp is arranged on the upper end face of the culture dish cover body, a bacterial suspension dropping hole is further formed in the culture dish cover body, a cover plate used for closing the bacterial suspension dropping hole is arranged on the culture dish cover body, a cavity is arranged in the coating rod mounting seat, a through hole which is communicated with the cavity and the lower end face of the coating rod mounting seat is formed in the bottom of the coating rod mounting seat, a connecting block is rotationally connected in the through hole and driven by a driving mechanism to rotate, the connecting block is detachably connected with the coating rod, the coating rod is made of an air bag, a rod head block is vertically and fixedly connected to the middle of the coating rod, a containing groove used for containing the coating rod is formed in the lower end face of the rod, the driving mechanism is arranged in the cavity of the coating rod mounting seat and is used for inflating and deflating the coating rod and driving the coating rod to rotate;
the driving mechanism comprises a speed regulating motor, a hollow gear and a hollow rotating shaft, the speed regulating motor is fixed in a cavity of the coating rod mounting seat, a transmission gear is connected to the rotating shaft of the speed regulating motor, a bearing is fixedly connected to the inside of the through hole, the hollow rotating shaft is rotatably connected with the bearing, the hollow rotating shaft is fixedly connected with a connecting block, blades are fixedly connected to the inner side wall of the hollow rotating shaft, the hollow gear is fixedly connected to the hollow rotating shaft and meshed with the transmission gear, and a ventilation opening communicated with the cavity is formed in the outer surface of the coating rod mounting seat.
2. The automated microbial coating apparatus of claim 1, wherein: limiting rings are respectively arranged on the side wall and the bottom of the coating rod mounting seat, and the culture dish cover is located between the two limiting rings.
3. The automated microbial coating apparatus of claim 1, wherein: and a bacterial suspension dropping pipe is sleeved in the bacterial suspension dropping hole.
4. The automated microbial coating apparatus of claim 1, wherein: the connecting block is cylindrical, magnet has been inlayed to the connecting block inside, the lower terminal surface of connecting block is equipped with the recess that agrees with the club head piece, the club head piece has magnetism, the recess top surface is equipped with air inlet and the gas outlet of intercommunication connecting block up end, all be equipped with the check valve on air inlet and the gas outlet, be equipped with the overflow mechanism that satisfies the threshold value and start on the gas outlet for prevent that the coating rod from being aerifyd excessively and giving the coating rod gassing and retrieving.
5. The automated microbial coating apparatus of claim 4, wherein: spill over the mechanism and include reset spring and baffle, the connecting block up end is equipped with the spacing groove, spacing groove bottom is located to the gas outlet, baffle sliding connection covers on the gas outlet at the spacing inslot, be equipped with the exhaust hole of staggering the setting with the gas outlet on the baffle, reset spring connects respectively between connecting block and baffle for make the baffle closely cover close on the gas outlet.
CN201811303391.9A 2018-11-02 2018-11-02 Automatic microorganism coating device Active CN109182103B (en)

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Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
CN201811303391.9A CN109182103B (en) 2018-11-02 2018-11-02 Automatic microorganism coating device

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CN109182103B true CN109182103B (en) 2021-06-29

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109593645A (en) * 2019-02-25 2019-04-09 江阴新雨生物科技有限公司 A kind of automatic microorganism killing strain apparatus for coating
CN114058488B (en) * 2021-12-15 2023-10-13 涌源合生科技(深圳)有限公司 Strain culture device based on enzyme engineering is convenient for coating

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5961846A (en) * 1996-02-28 1999-10-05 Marshfield Medical Research And Education Foundation Concentration of waterborn and foodborn microorganisms
JP5326117B2 (en) * 2008-08-25 2013-10-30 株式会社ＡＭＢｉＳ Antifoaming device, culture system and container with antifoaming device
CN203264985U (en) * 2013-06-09 2013-11-06 三峡大学 Rapid microorganism coating device
CN206591120U (en) * 2017-03-13 2017-10-27 苏州金唯智生物科技有限公司 A kind of apparatus for coating
CN207330933U (en) * 2017-10-23 2018-05-08 四川农业大学 A kind of microculture tablet apparatus for coating
CN207685289U (en) * 2017-12-19 2018-08-03 河南科技学院 A kind of microorganism apparatus for coating reducing contamination rate

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5961846A (en) * 1996-02-28 1999-10-05 Marshfield Medical Research And Education Foundation Concentration of waterborn and foodborn microorganisms
JP5326117B2 (en) * 2008-08-25 2013-10-30 株式会社ＡＭＢｉＳ Antifoaming device, culture system and container with antifoaming device
CN203264985U (en) * 2013-06-09 2013-11-06 三峡大学 Rapid microorganism coating device
CN206591120U (en) * 2017-03-13 2017-10-27 苏州金唯智生物科技有限公司 A kind of apparatus for coating
CN207330933U (en) * 2017-10-23 2018-05-08 四川农业大学 A kind of microculture tablet apparatus for coating
CN207685289U (en) * 2017-12-19 2018-08-03 河南科技学院 A kind of microorganism apparatus for coating reducing contamination rate

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