CN109172866A - A kind of drying biological cardiac valves and preparation method thereof for the flattening that can quickly absorb water - Google Patents
A kind of drying biological cardiac valves and preparation method thereof for the flattening that can quickly absorb water Download PDFInfo
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- CN109172866A CN109172866A CN201811095786.4A CN201811095786A CN109172866A CN 109172866 A CN109172866 A CN 109172866A CN 201811095786 A CN201811095786 A CN 201811095786A CN 109172866 A CN109172866 A CN 109172866A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3625—Vascular tissue, e.g. heart valves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/18—Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/20—Materials or treatment for tissue regeneration for reconstruction of the heart, e.g. heart valves
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
Abstract
The invention discloses a kind of drying biological cardiac valves and preparation method thereof of flattening that can quickly absorb water, the preparation method includes: that animal pericardium is carried out crosslinking Treatment using glutaraldehyde water solution, it is impregnated in extracellular matrix biology enzyme aqueous solution later, it finally dries, the drying biological cardiac valves for the flattening that obtains quickly absorbing water.Have good quick water suction from performance is calmed down using dry biological cardiac valves made from preparation method of the present invention, can quickly absorb water flattening under simulation crimping test.
Description
Technical field
The present invention relates to the dry biologies of medical material and medical instruments field, more particularly to a kind of flattening that can quickly absorb water
Heart valve and preparation method thereof.
Background technique
Heart valve disease is a kind of common valve wasting conditions.Blood access is anatomically shown as to narrow or valve
Film incompetence.The treatment of heart valve disease includes opening chest valve replacement surgery and percutaneous heart valve replacement operation.It opens
Chest operation is big to patient trauma, risk is high, recovery is slow, extracorporal circulatory system is needed to support, many patients can not receive.Percutaneous heart valve
Replacement operation becomes the main trend of the following valve surgery because of small to patient trauma, risk is low.Biological cardiac valves refer to one
Class is for replacing the valvular biomedical material of human lesion.Biological cardiac valves are generally by Pigs Hearts coating, bovine pericardium
It is prepared Deng by glutaraldehyde cross-linking.
Existing intervention biovalve generally uses glutaraldehyde cross-linking, then sews biovalve in a kind of metallic support
On, then it is stored in glutaraldehyde solution.Before carrying out intervention valve replacement surgery, need to hold by being cleaned multiple times, pressing
And assembling.This preparation process is cumbersome, and operation additional risk is easy to cause to increase.The life in glutaraldehyde solution is deposited in for a long time
Object valve is easy to cause the residual of glutaraldehyde, increases the calcification and toxicity of biovalve.Therefore, biovalve is developed into
It is detached from the dry film that glutaraldehyde solution saves, and is pre-loaded in valve delivery system, can preferably be solved the above problems.But
It is the drying biovalve being pre-loaded in valve delivery system, due to the mechanical backfin of long period to be subjected to, because
To the quick water suction of the biovalve under drying regime, more stringent requirements are proposed from calming down performance, existing biovalve without
Method quickly absorbs water flattening.
Therefore, the existing technology needs to be improved and developed.
Summary of the invention
In view of above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a kind of dry lifes of flattening that can quickly absorb water
Object heart valve and preparation method thereof, it is intended to solve existing intervention biovalve need to be stored in glutaraldehyde solution, nothing
Method is pre-loaded in transportation system, needs temporarily to clean pressure and hold assembling and the problem of the flattening that can not quickly absorb water.
Technical scheme is as follows:
The present invention provides a kind of preparation methods of the drying biological cardiac valves of flattening that can quickly absorb water, wherein including
Step: animal pericardium is subjected to crosslinking Treatment using glutaraldehyde water solution, later in extracellular matrix biology enzyme aqueous solution
It impregnates, finally dries, the drying biological cardiac valves for the flattening that obtains quickly absorbing water.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein the extracellular matrix
Biological enzyme aqueous solution is one of Collagenase, elastoser, hyaluronidase, chondrosulphatase or a variety of mixing
Solution.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein the extracellular matrix
The concentration of biological enzyme aqueous solution is 0.1~50U/ml.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein after crosslinking Treatment
Animal pericardium impregnates 12~72h in extracellular matrix biology enzyme aqueous solution under 25~37 DEG C of oscillating condition.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein the glutaraldehyde is water-soluble
The volumetric concentration of liquid is that 0.1~5%, pH is 7~8.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein adopt animal pericardium
With glutaraldehyde water solution under 20~45 DEG C of oscillating condition crosslinking Treatment 1~7 day.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein the drying refers to
It is dehydrated in alcohols solvent.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein the dehydration and drying is adopted
With the dehydration and drying of gradient concentration alcohols mixed solvent.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein the alcohols solvent packet
Include methanol, ethyl alcohol, normal propyl alcohol, isopropanol, glycerine, n-butanol, n-amyl alcohol, n-undecane alcohol, n-dodecane alcohol, 2- propyl alcohol,
2- butanol, 2- hexanol, cyclohexanol, the tert-butyl alcohol it is one or more.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein animal pericardium exists
25% ethyl alcohol/25% isopropanol/50% 20~30h of glycerine, 45% ethyl alcohol/45% isopropyl are successively soaked at 25~45 DEG C
It is dry to complete serial dehydration for alcohol/10% 20~30h of glycerine.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein adopt animal pericardium
It further include that animal pericardium is carried out to de- cell processing before carrying out crosslinking Treatment with glutaraldehyde water solution.
The preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water, wherein adopt animal pericardium
Before carrying out crosslinking Treatment with glutaraldehyde water solution, the revolving speed by fresh animal pericardium in 3~5 DEG C, 80~120RPM shakes
1.5~3h is cleaned with distilled water under the conditions of swinging.
The present invention also provides a kind of drying biological cardiac valves of flattening that can quickly absorb water, wherein uses such as to take up an official post
Preparation method described in one is made.
The utility model has the advantages that the present invention provides a kind of drying biological cardiac valves of flattening that can quickly absorb water and its preparation sides
Method using dry biological cardiac valves made from preparation method of the present invention there is good quick water suction to calm down performance certainly,
Can quickly absorb water flattening under simulation crimping test.
Specific embodiment
The present invention provides a kind of drying biological cardiac valves and preparation method thereof of flattening that can quickly absorb water, to make the present invention
Purpose, technical solution and effect it is clearer, clear, the present invention is described in more detail below.It should be appreciated that this place
The specific embodiment of description is only used to explain the present invention, is not intended to limit the present invention.
A kind of preparation method of the drying biological cardiac valves for flattening that can quickly absorb water that present pre-ferred embodiments provide,
It that is to say that one kind can pre-install dry biological cardiac valves and quickly absorb water from the improved method calmed down, wherein comprising steps of by animal
Pericardium carries out crosslinking Treatment using glutaraldehyde water solution, impregnates in extracellular matrix biology enzyme aqueous solution, finally does later
It is dry, the drying biological cardiac valves for the flattening that obtains quickly absorbing water.Wherein, it is preferred that the drying refers in alcohols solvent
Dehydration and drying.
The present invention concentrates on the quick water suction for improving dry biovalve from calming down performance, to reach this purpose, the present invention
Creative will be added biological enzyme in the animal pericardium handled through glutaraldehyde cross-linking, keep bioprosthesis valve tissue fast to play
Speed covers the effect of water, to obtain pre-installing dry biological cardiac valves from calm down performance with quickly water suction.
Generally, preparation method of the present invention includes: the crosslinking of 1. biovalves；2. the water suction of biovalve improves
Biochemistry treatment；3. the dehydration and drying of gradient concentration alcohols solvent.Wherein, the water suction of biovalve improves biochemistry treatment
Dehydration and drying with gradient concentration alcohols solvent is committed step of the invention.
Further, animal pericardium of the present invention includes but is not limited to pig pericardium heart valve, bovine pericardium valve etc..This reality
It applies in example, further includes taking off animal pericardium before animal pericardium is carried out crosslinking Treatment using glutaraldehyde water solution
Cell processing, and the step of animal pericardium is cleaned up.When it is implemented, acquiring the pericardium group of fresh pig or ox
It knits, and is saved under 4 DEG C of low temperature moisture states.Using it is soft friction and Fluid pressure under oscillating condition wash with distilled water
Pericardial tissue, remove adherency non-pericardium and non-collagen tissue.Cleaning of the present invention is realized by osmotic shock to pericardium group
It is woven with the de- cell of effect, preferably cleans the non-pericardium for continueing to no visible adherency or non-collagen tissue.When it is implemented, it is preferred that
, described the step of cleaning up animal pericardium includes: by fresh animal pericardium in 3~5 DEG C, 80~120RPM
1.5~3h is cleaned with distilled water under the conditions of speed oscillation.For example, can under 4 DEG C of 100RPM speed oscillation conditions distilled water
2h is cleaned, the fresh animal cleaned up (pig or ox) pericardium is then immersed into glutaraldehyde water solution and carries out crosslinking Treatment.
Further, in the present embodiment, the volumetric concentration of the glutaraldehyde water solution is preferably 0.1~5%, such as can be with
It is 0.1%, 1%, 5% etc., the pH of the glutaraldehyde water solution is preferably 7~8, such as can be 7,7.4,8 etc..Preferably,
Animal pericardium is used into glutaraldehyde water solution crosslinking Treatment 1~7 day under 20~45 DEG C of oscillating condition.This step is biology
The crosslinking of valve will realize that stablizing for most of collagen tissue is crosslinked, improve the structural stability of entire pericardial tissue, reduce with
As for elimination immunogenicity.
Further, in the present embodiment, the extracellular matrix biology enzyme aqueous solution is Collagenase, elastin laminin
One of enzyme, hyaluronidase, chondrosulphatase or a variety of mixed solutions.The extracellular matrix biology enzyme aqueous solution
Concentration is preferably 0.1~50U/ml, such as can be 0.1U/ml, 1U/ml, 20U/ml, 50U/ml etc..It preferably, will be at crosslinking
Animal pericardium after reason impregnates 12~72h in extracellular matrix biology enzyme aqueous solution under 25~37 DEG C of oscillating condition.
This step is that the water suction of biovalve improves biochemistry treatment, therefore, to assure that extracellular matrix biological enzyme reaches close to saturation
Physical penetration, to introduce extracellular matrix biological enzyme as much as possible.
Further, in the present embodiment, the dehydration and drying of the present invention in alcohols solvent preferably uses gradient concentration
The dehydration and drying of alcohols mixed solvent.The alcohols solvent includes but is not limited to methanol, ethyl alcohol, normal propyl alcohol, isopropanol, the third three
In alcohol, n-butanol, n-amyl alcohol, n-undecane alcohol, n-dodecane alcohol, 2- propyl alcohol, 2- butanol, 2- hexanol, cyclohexanol, tert-butyl alcohol etc.
One or more mixing.When it is implemented, it is preferred, animal pericardium is successively soaked in 25% second at 25~45 DEG C
Alcohol/25% isopropanol/50% 20~30h of glycerine (such as 24 hours), the glycerine of 45% ethyl alcohol/45% isopropanol/10% 20~
30h (such as 24 hours), the serial dehydration for completing animal pericardium is dry.
The embodiment of the invention also provides a kind of drying biological cardiac valves of flattening that can quickly absorb water, wherein using such as
Preparation method described in any of the above item is made.Had using dry biological cardiac valves made from preparation method of the present invention
Good quick water suction is from performance is calmed down, and can quickly absorb water flattening under simulation crimping test.
The present invention is pre-installed dry biological cardiac valves and is quickly absorbed water from calming down improved technology by unique, so that preparation
Drying biological cardiac valves have good water suction from calming down performance after being implanted into human body, can be fast under simulation crimping test
Speed water suction flattening.Be detached from glutaraldehyde solution preservation pre-installs dry biovalve.The residual of glutaraldehyde can be reduced, reduces penta
Dialdehyde bring calcification problem and toxicity simplify the preoperative installation of valve system, reduce the additional risk of operation.
It is elaborated below with specific embodiment to the present invention:
Embodiment 1
Fresh pig pericardium comes from local slaughterhouse.Glutaraldehyde is from Chengdu bass spy Reagent Company.Collagenase
From Sigma-Aldrich company.By the Pigs Hearts coating cleaned up 1% volumetric concentration glutaraldehyde water solution (pH
7.4,25 DEG C) crosslinking 48h.Distilled water cleaning.Then it is impregnated for 24 hours at 37 DEG C in the collagen enzyme aqueous solution of 10U/ml.
Then 25% ethyl alcohol/25% isopropanol/50% glycerine is successively soaked under 25 DEG C of conditions for 24 hours, 45% ethyl alcohol/45% is different
Propyl alcohol/10% glycerine for 24 hours, realizes the serial dehydration of biovalve.Being prepared can from calm down performance with good water suction
Pre-install dry biological cardiac valves.
It is found through detection, drying biological cardiac valves (doubling two under simulation crimping test condition of this implementation preparation
10kg weight crimping 30 days after secondary) it can quickly be flattened within immersion 3min, without obvious Folding damage.Uniaxial tensile test
Under (1*5cm sample size) tensile break stress be greater than 15N.
Embodiment 2
Fresh pig pericardium comes from local slaughterhouse.Glutaraldehyde is from Chengdu bass spy Reagent Company.Elastoser
From Shanghai Aladdin biochemical technology limited liability company.By the Pigs Hearts coating cleaned up the penta 2 of 1% volumetric concentration
Aldehyde aqueous solution (7.4,25 DEG C of pH) is crosslinked 48h.Distilled water cleaning.Then 37 DEG C in the elastin laminin enzyme aqueous solution of 5U/ml
Lower immersion is for 24 hours.Then 25% ethyl alcohol/25% isopropanol/50% glycerine is successively soaked under 25 DEG C of conditions for 24 hours, 45%
Ethyl alcohol/45% isopropanol/10% glycerine for 24 hours, realizes the serial dehydration of biovalve.Being prepared has good water suction certainly flat
Renaturation energy pre-installs dry biological cardiac valves.
It is found through detection, drying biological cardiac valves (doubling two under simulation crimping test condition of this implementation preparation
10kg weight crimping 30 days after secondary) it can quickly be flattened within immersion 3min, without obvious Folding damage.Uniaxial tensile test
Under (1*5cm sample size) tensile break stress be greater than 15N.
Embodiment 3
Fresh pig pericardium comes from local slaughterhouse.Glutaraldehyde is from Chengdu bass spy Reagent Company.Elastoser,
Hyaluronidase is from Shanghai Aladdin biochemical technology limited liability company.By the Pigs Hearts coating cleaned up in 1% volume
The glutaraldehyde water solution (7.4,25 DEG C of pH) of concentration is crosslinked 48h.Distilled water cleaning.Then in the elastoser of 1U/ml and
It is impregnated for 24 hours at 37 DEG C in the hyaluronidase mixed aqueous solution of 5U/ml.Then 25% is successively soaked under 25 DEG C of conditions
For 24 hours, 45% ethyl alcohol/45% isopropanol/10% glycerine for 24 hours, realizes biovalve to ethyl alcohol/25% isopropanol/50% glycerine
Serial dehydration.It is prepared and pre-installs dry biological cardiac valves from calm down performance with good water suction.
It is found through detection, drying biological cardiac valves (doubling two under simulation crimping test condition of this implementation preparation
10kg weight crimping 30 days after secondary) it can quickly be flattened within immersion 3min, without obvious Folding damage.Uniaxial tensile test
Under (1*5cm sample size) tensile break stress be greater than 15N.
Embodiment 4
Fresh pig pericardium comes from local slaughterhouse.Glutaraldehyde is from Chengdu bass spy Reagent Company.Elastoser,
Hyaluronidase is from Shanghai Aladdin biochemical technology limited liability company.By the Pigs Hearts coating cleaned up in 1% volume
The glutaraldehyde water solution (7.4,25 DEG C of pH) of concentration is crosslinked 48h.Distilled water cleaning.Then in the chondrosulphatase of 50U/ml
It is impregnated for 24 hours at 37 DEG C in mixed aqueous solution.Then be successively soaked under 25 DEG C of conditions 25% ethyl alcohol/25% isopropanol/
For 24 hours, 45% ethyl alcohol/45% isopropanol/10% glycerine for 24 hours, realizes the serial dehydration of biovalve to 50% glycerine.It is prepared into
To having good water suction to pre-install dry biological cardiac valves from calm down performance.
It is found through detection, drying biological cardiac valves (doubling two under simulation crimping test condition of this implementation preparation
10kg weight crimping 30 days after secondary) it can quickly be flattened within immersion 3min, without obvious Folding damage.Uniaxial tensile test
Under (1*5cm sample size) tensile break stress be greater than 15N.
Embodiment 5
Fresh pig pericardium comes from local slaughterhouse.Glutaraldehyde is from Chengdu bass spy Reagent Company.Elastoser,
Hyaluronidase is from Shanghai Aladdin biochemical technology limited liability company.By the Pigs Hearts coating cleaned up in 1% volume
The glutaraldehyde water solution (7.4,25 DEG C of pH) of concentration is crosslinked 48h.Distilled water cleaning.Then in the Collagenase of 50U/ml and
It is impregnated for 24 hours at 37 DEG C in chondrosulphatase mixed aqueous solution.Then be successively soaked under 25 DEG C of conditions 25% ethyl alcohol/
For 24 hours, 45% ethyl alcohol/45% isopropanol/10% glycerine for 24 hours, realizes the gradient of biovalve to 25% isopropanol/50% glycerine
Dehydration.It is prepared and pre-installs dry biological cardiac valves from calm down performance with good water suction.
It is found through detection, drying biological cardiac valves (doubling two under simulation crimping test condition of this implementation preparation
10kg weight crimping 30 days after secondary) it can quickly be flattened within immersion 3min, without obvious Folding damage.Uniaxial tensile test
Under (1*5cm sample size) tensile break stress be greater than 15N.
Embodiment 6
Fresh pig pericardium comes from local slaughterhouse.Glutaraldehyde is from Chengdu bass spy Reagent Company.Elastoser,
Hyaluronidase is from Shanghai Aladdin biochemical technology limited liability company.By the Pigs Hearts coating cleaned up in 1% volume
The glutaraldehyde water solution (7.4,25 DEG C of pH) of concentration is crosslinked 48h.Distilled water cleaning.Then in the Collagenase of 0.1U/ml and
It is impregnated for 24 hours at 37 DEG C in chondrosulphatase mixed aqueous solution.Then be successively soaked under 25 DEG C of conditions 25% ethyl alcohol/
For 24 hours, 45% ethyl alcohol/45% isopropanol/10% glycerine for 24 hours, realizes the gradient of biovalve to 25% isopropanol/50% glycerine
Dehydration.It is prepared and pre-installs dry biological cardiac valves from calm down performance with good water suction.
It is found through detection, drying biological cardiac valves (doubling two under simulation crimping test condition of this implementation preparation
10kg weight crimping 30 days after secondary) it can quickly be flattened within immersion 3min, without obvious Folding damage.Uniaxial tensile test
Under (1*5cm sample size) tensile break stress be greater than 15N.
In conclusion the present invention provides a kind of drying biological cardiac valves of flattening that can quickly absorb water and its preparation sides
Method using dry biological cardiac valves made from preparation method of the present invention there is good quick water suction to calm down performance certainly,
Can quickly absorb water flattening under simulation crimping test.Be detached from that glutaraldehyde solution saves pre-installs dry biovalve, can be with
The residual of glutaraldehyde is reduced, glutaraldehyde bring calcification problem and toxicity are reduced, simplifies the preoperative installation of valve system, is reduced
The additional risk of operation.
It should be understood that the application of the present invention is not limited to the above for those of ordinary skills can
With improvement or transformation based on the above description, all these modifications and variations all should belong to the guarantor of appended claims of the present invention
Protect range.
Claims (13)
- The preparation method of the drying biological cardiac valves of flattening 1. one kind can quickly absorb water, which is characterized in that comprising steps ofAnimal pericardium is subjected to crosslinking Treatment using glutaraldehyde water solution, is soaked in extracellular matrix biology enzyme aqueous solution later Bubble, is finally dried, the drying biological cardiac valves for the flattening that obtains quickly absorbing water.
- 2. the preparation method of the drying biological cardiac valves of the flattening according to claim 1 that can quickly absorb water, feature exist In the extracellular matrix biology enzyme aqueous solution is Collagenase, elastoser, hyaluronidase, chondrosulphatase One of or a variety of mixed solutions.
- 3. the preparation method of the drying biological cardiac valves of the flattening according to claim 1 that can quickly absorb water, feature exist In the concentration of the extracellular matrix biology enzyme aqueous solution is 0.1~50U/ml.
- 4. the preparation method of the drying biological cardiac valves of the flattening according to claim 3 that can quickly absorb water, feature exist In the animal pericardium after crosslinking Treatment is soaked under 25~37 DEG C of oscillating condition in extracellular matrix biology enzyme aqueous solution Steep 12~72h.
- 5. the preparation method of the drying biological cardiac valves of the flattening according to claim 1 that can quickly absorb water, feature exist In the volumetric concentration of the glutaraldehyde water solution is that 0.1~5%, pH is 7~8.
- 6. the preparation method of the drying biological cardiac valves of the flattening according to claim 5 that can quickly absorb water, feature exist In by animal pericardium using glutaraldehyde water solution crosslinking Treatment 1~7 day under 20~45 DEG C of oscillating condition.
- 7. the preparation method of the drying biological cardiac valves of the flattening according to claim 1 that can quickly absorb water, feature exist In the drying refers to be dehydrated in alcohols solvent.
- 8. the preparation method of the drying biological cardiac valves of the flattening according to claim 7 that can quickly absorb water, feature exist In the dehydration and drying uses the dehydration and drying of gradient concentration alcohols mixed solvent.
- 9. the preparation method of the drying biological cardiac valves of the flattening according to claim 7 that can quickly absorb water, feature exist In, the alcohols solvent include methanol, ethyl alcohol, normal propyl alcohol, isopropanol, glycerine, n-butanol, n-amyl alcohol, n-undecane alcohol, just Dodecanol, 2- propyl alcohol, 2- butanol, 2- hexanol, cyclohexanol, the tert-butyl alcohol it is one or more.
- 10. the preparation method of the drying biological cardiac valves of the flattening that can quickly absorb water according to claim 8 or claim 9, special Sign is, animal pericardium is successively soaked at 25~45 DEG C the glycerine of 25% ethyl alcohol/25% isopropanol/50% 20~ It is dry to complete serial dehydration for 30h, 45% ethyl alcohol/45% isopropanol/10% 20~30h of glycerine.
- 11. the preparation method of the drying biological cardiac valves of the flattening according to claim 1 that can quickly absorb water, feature exist In further including that animal pericardium is carried out to de- cell before animal pericardium is carried out crosslinking Treatment using glutaraldehyde water solution Processing.
- 12. the preparation method of the drying biological cardiac valves of the flattening according to claim 1 that can quickly absorb water, feature exist In before animal pericardium is carried out crosslinking Treatment using glutaraldehyde water solution, by fresh animal pericardium in 3~5 DEG C, 80 1.5~3h is cleaned with distilled water under the conditions of the speed oscillation of~120RPM.
- The drying biological cardiac valves of flattening 13. one kind can quickly absorb water, which is characterized in that using such as claim 1~12 times Preparation method described in one is made.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811095786.4A CN109172866A (en) | 2018-09-19 | 2018-09-19 | A kind of drying biological cardiac valves and preparation method thereof for the flattening that can quickly absorb water |
| PCT/CN2019/105361 WO2020057414A1 (en) | 2018-09-19 | 2019-09-11 | Dry biological heart valve capable of rapidly absorbing water and flattening, and preparation method therefor |
Applications Claiming Priority (1)
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| CN201811095786.4A CN109172866A (en) | 2018-09-19 | 2018-09-19 | A kind of drying biological cardiac valves and preparation method thereof for the flattening that can quickly absorb water |
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| CN110101912A (en) * | 2019-05-16 | 2019-08-09 | 四川大学 | A kind of drying artificial biological heart valve and preparation method thereof of the endothelialization polypeptide containing promotion |
| CN110101911A (en) * | 2019-05-16 | 2019-08-09 | 四川大学 | A kind of drying artificial biological heart valve and preparation method thereof of the endothelialization growth factor containing promotion |
| CN110613865A (en) * | 2019-11-07 | 2019-12-27 | 四川大学 | Preparation and storage method of biological valve material subjected to combined treatment of carbodiimide and polyphenol |
| WO2020057414A1 (en) * | 2018-09-19 | 2020-03-26 | 杭州启明医疗器械股份有限公司 | Dry biological heart valve capable of rapidly absorbing water and flattening, and preparation method therefor |
| CN111567514A (en) * | 2020-04-20 | 2020-08-25 | 上海以心医疗器械有限公司 | Method for preserving fresh biological tissue |
| CN115335094A (en) * | 2020-04-15 | 2022-11-11 | 维他有限责任公司 | Method for processing collagen-based tissue for bioprosthetic devices |
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| WO2020057414A1 (en) * | 2018-09-19 | 2020-03-26 | 杭州启明医疗器械股份有限公司 | Dry biological heart valve capable of rapidly absorbing water and flattening, and preparation method therefor |
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| CN115335094A (en) * | 2020-04-15 | 2022-11-11 | 维他有限责任公司 | Method for processing collagen-based tissue for bioprosthetic devices |
| CN111567514A (en) * | 2020-04-20 | 2020-08-25 | 上海以心医疗器械有限公司 | Method for preserving fresh biological tissue |
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| WO2020057414A1 (en) | 2020-03-26 |
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Address after: 310000 Room 311, 3/F, Building No. 88, Jiangling Road, Binjiang District, Hangzhou City, Zhejiang Province Applicant after: Hangzhou Qiming Medical Devices Co.,Ltd. Applicant after: SICHUAN University Address before: 310000 Room 311, 3/F, Building No. 88, Jiangling Road, Binjiang District, Hangzhou City, Zhejiang Province Applicant before: VENUS MEDTECH (HANGZHOU), Inc. Applicant before: Sichuan University |
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Application publication date: 20190111 |