CN109171002B

CN109171002B - Sodium borate buffer solution for reducing TSNAs in cigarette smoke

Info

Publication number: CN109171002B
Application number: CN201811076835.XA
Authority: CN
Inventors: 刘兴余; 周骏; 王金明; 刘德水; 杨振东; 白若石; 马雁军; 严莉红; 马莉; 张�杰; 徐同广; 卢楠; 张晨; 杜国荣
Original assignee: Shanghai Tobacco Group Co Ltd
Current assignee: Shanghai Tobacco Group Co Ltd
Priority date: 2018-09-14
Filing date: 2018-09-14
Publication date: 2022-04-29
Anticipated expiration: 2038-09-14
Also published as: CN109171002A

Abstract

The invention relates to a sodium borate buffer solution for reducing TSNAs in cigarette smoke, which comprises boric acid and borax; wherein the spraying amount of the boric acid is 0.2-0.7% and the spraying amount of the borax is 0.3-1.5% based on the dry weight of the tobacco flakes. The sodium borate buffer solution is sprayed on the surface of a tobacco sheet, and then the tobacco sheet is made into a cigarette; the release amount of TSNAs in the smoke of the prepared cigarette is obviously lower than that of the cigarette sample without applying the sodium borate buffer solution.

Description

Sodium borate buffer solution for reducing TSNAs in cigarette smoke

Technical Field

The invention relates to a sodium borate buffer solution for reducing TSNAs in cigarette smoke.

Background

TSNAs (TSNAs, Tobacco Specific N-Nitrosamines) is a Specific carcinogenic component in Tobacco, has slow metabolism speed in human body, long half-life period and great harm to human health. Thus, TSNAs are included in a list of tobacco harmful components, one of the harmful components in cigarettes that needs to be disclosed and reduced. Currently, people mainly pay attention to the following 4 types: 4- (N-methyl-nitrosamine) -1- (3-pyridyl) -1-butanone (NNK, 4- (N-methyl-N-nitrosamine) -1- (3-pyridol) -1-butanone), N-Nitrosonornicotine (NNN, N ' -nitrosinonornicotine), N-nitrosoneonicotine (NAT, N ' -nitrosoanataine), N-nitrosopseudoequisetine (NAB, N ' -nitrosoanabaseine).

TSNAs are mainly generated in the preparation, alcoholization and processing stages of tobacco, once formed, TSNAs can stably exist in tobacco and can transfer part of TSNAs to cigarette smoke when the tobacco is burnt. In addition, TSNAs are also formed by thermal cracking reactions during the combustion of tobacco. Thus, the TSNAs in the cigarette smoke are derived in part from the direct transfer in tobacco and in part from the thermal cracking reaction of the tobacco combustion process. The thermal cracking reaction in the cigarette combustion process is influenced by the tobacco exogenous additive, and different tobacco additives influence the combustion reaction of tobacco, so that the TSNAs release amount in the cigarette smoke is different. At present, exogenous compounds such as nano materials, catalysts and the like are applied to tobacco leaves to change the combustion state of the tobacco, influence thermal cracking reaction and reduce TSNAs in cigarette smoke, but reports that sodium borate buffer solution is added to the tobacco to reduce TSNAs in the cigarette smoke are not found.

Disclosure of Invention

The invention aims to provide a sodium borate buffer solution for reducing TSNAs in cigarette smoke. The sodium borate buffer is an inorganic salt, and reports on the reduction of specific N-nitrosamine in cigarette smoke are not found. The sodium borate buffer solution comprises boric acid and borax.

In order to reduce the TSNAs content to the maximum extent and effectively ensure the taste of the cigarettes, the invention limits the maximum spraying amount of boric acid to be 0.7 percent of the dry basis weight of the tobacco leaves and the maximum spraying amount of borax to be 1.5 percent of the dry basis weight of the tobacco leaves. Preferably; the sodium borate buffer solution is characterized in that the spraying amount of boric acid is 0.2-0.7% and the spraying amount of borax is 0.3-1.5% based on the dry weight of the tobacco lamina.

The sodium borate buffer solution takes water as a solvent, and contains 2-3.5% of boric acid and 4-5% of borax by weight volume percentage;

preferably, the solvent is water, and the boric acid is 2.2-3.08% and the borax is 4.4-4.6%. The sodium borate buffer solution prepared according to the proportion can effectively reduce TSNAs in smoke, can change the pH value of tobacco raw materials, and has certain capacity of buffering the pH value of tobacco.

In order to further illustrate that the sodium borate buffer solution can effectively reduce TSNAs in cigarette smoke, the invention provides a method for reducing TSNAs in cigarette smoke, which comprises the following specific steps: uniformly spraying the sodium borate buffer solution on the surface of the tobacco flakes;

preferably, the spraying amount of the sodium borate buffer solution is calculated by the dry basis weight of the tobacco flakes, and 10-20 mL of the sodium borate buffer solution is sprayed every 100g of tobacco flakes; preferably 15-20 mL; most preferably, 20mL of the sodium borate buffer solution is sprayed every 100g of tobacco flakes;

optionally, the tobacco flakes are sun-cured tobacco.

After the sodium borate buffer solution is sprayed on the tobacco flakes, in order to enable the tobacco flakes to have good effects, the tobacco flakes are naturally dried in an environment with the temperature of 20-25 ℃ and the relative humidity of 50-70% until the moisture content of tobacco leaves is 10-15%.

Preferably, the tobacco flakes are naturally dried in an environment with the temperature of 22 ℃ and the relative humidity of 60% until the moisture content of the tobacco leaves is 12%;

and then cutting the sheet tobacco into tobacco shreds, and preparing the cigarette.

The invention provides a preferable scheme, and the method specifically comprises the following steps: spraying 15-20 mL of the sodium borate buffer solution per 100g of tobacco flakes, and uniformly spraying the sodium borate buffer solution to the surfaces of the tobacco flakes; and naturally drying the tobacco flakes until the moisture content of the tobacco leaves is 10-15%, and then cutting the tobacco flakes into tobacco shreds to prepare cigarettes.

Preferably, 20mL of the sodium borate buffer solution is sprayed every 100g of tobacco flakes, and the sodium borate buffer solution is uniformly sprayed on the surfaces of the tobacco flakes; then naturally drying the tobacco sheets until the moisture content of the tobacco leaves is 12%, and then cutting the tobacco sheets into tobacco shreds to prepare cigarettes;

more preferably, the sodium borate buffer solution takes water as a solvent and contains 2.2-3.08% of boric acid and 4.4-4.6% of borax in weight-volume ratio.

Detailed Description

The present invention is further illustrated below with reference to specific examples, which are intended to be illustrative only and not to limit the scope of the invention.

The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.

Reagents were used in the following examples:

boric acid (AR grade, available from Beijing Bailingwei science and technology Co., Ltd.)

Borax (AR grade, available from Beijing Bailingwei science and technology Co., Ltd.)

4 TSNAs standards and their internal standards (purity 98% or more, available from Torto Research Chemicals Inc.)

The adopted instrument is as follows:

high performance liquid chromatography-triple quadrupole tandem mass spectrometer (high performance liquid chromatography: Symbiosis (Pico), available from Spark Holland;

triple quadrupole mass spectrometer: API5500, available from applied biosystems, usa.

Example 1

The embodiment provides a sodium borate buffer solution for reducing TSNAs in cigarette smoke, which takes clear water as a solvent, and 0.022g of boric acid and 0.044g of borax are added into every 1mL of clear water.

Example 2

The embodiment provides a sodium borate buffer solution for reducing TSNAs in cigarette smoke, which takes clear water as a solvent, and 0.03g of boric acid and 0.046g of borax are added into every 1mL of clear water.

Example 3

The embodiment provides a method for reducing TSNAs in cigarette smoke, which specifically comprises the following steps:

1) 200mL of the sodium borate buffer solution prepared in the embodiment 1 is uniformly sprayed to the surface of 1kg of threshed and redried burley sheet tobacco in a mist form;

2) and (3) placing the sprayed tobacco flakes in an environment with the temperature of 22 ℃ and the relative humidity of 60-70%, shredding when the moisture content of the tobacco leaves is 12%, and rolling into cigarettes.

Example 4

1) 200mL of the sodium borate buffer solution prepared in the embodiment 2 is uniformly sprayed to the surface of 1kg of threshed and redried burley tobacco in a mist form;

2) and (3) placing the sprayed tobacco flakes in an environment with the temperature of 22 ℃ and the relative humidity of 60%, shredding when the moisture content of the tobacco leaves is 12%, and rolling into cigarettes.

Comparative example 1

This comparative example is compared with examples 3 and 4, except that the sodium sparge buffer solution was changed to the same volume of clear water and the other operating steps were the same.

Examples of the experiments

The experimental method comprises the following steps: the cigarettes prepared in the examples 3, 4 and 1 are balanced in an environment with 22 ℃ and 60% relative humidity for 48 hours, then a smoking experiment is carried out according to the ISO 4387:2000 standard, and the TSNAs release amount in the mainstream smoke of the cigarettes is detected according to the GB/T23228 + 2008 standard.

The results of the experiment are shown in table 1: wherein, the reduction rate (%) of TSNAs is (TSNAs release amount in cigarette smoke of control sample-TSNAs release amount in cigarette smoke of example sample)/TSNAs release amount in cigarette smoke of control sample)) × 100%.

TABLE 1 Release amounts of TSNAs in cigarette mainstream Smoke

From the above, the release amount of TSNAs in the cigarette smoke prepared by the method of the invention can be obviously reduced.

Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. A method for reducing TSNAs in cigarette smoke is characterized in that a sodium borate buffer solution is uniformly sprayed on the surface of a burley tobacco sheet, wherein the sodium borate buffer solution comprises boric acid and borax; wherein the spraying amount of the boric acid is 0.2-0.7% and the spraying amount of the borax is 0.3-1.5% by taking the dry basis weight of the burley tobacco sheet;

after the burley tobacco flake tobacco is sprayed with the sodium borate buffer solution, the burley tobacco flake tobacco is naturally dried in an environment with the temperature of 20-25 ℃ and the relative humidity of 50-70% until the moisture content of the tobacco leaves is 10-15%.

2. The method according to claim 1, wherein after the burley tobacco lamina is sprayed with the sodium borate buffer, the burley tobacco lamina is naturally dried in an environment with the temperature of 22 ℃ and the relative humidity of 60% until the moisture content of tobacco leaves is 12%.

3. The method according to claim 1, wherein the sodium borate buffer is uniformly sprayed on the surface of the burley tobacco lamina in an amount of 15-20 mL of the sodium borate buffer per 100g of burley tobacco lamina; and naturally drying the burley tobacco sheet until the moisture content of the tobacco leaves is 10-15%, and then cutting into tobacco shreds to prepare the cigarette.

4. The method according to claim 3, wherein the sodium borate buffer is uniformly sprayed onto the surface of burley tobacco lamina in an amount of 20mL of the sodium borate buffer per 100g of burley tobacco lamina; and then naturally drying the burley tobacco sheet until the moisture content of the tobacco leaves is 12%, and then cutting into tobacco shreds to prepare the cigarette.

5. The method according to claim 4, wherein the sodium borate buffer is prepared by using water as a solvent and comprises 2.2-3.08% of boric acid and 4.4-4.6% of borax by weight/volume ratio.