CN109158137A - A kind of preparation method of micro-fluidic chip - Google Patents
A kind of preparation method of micro-fluidic chip Download PDFInfo
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- CN109158137A CN109158137A CN201811258313.1A CN201811258313A CN109158137A CN 109158137 A CN109158137 A CN 109158137A CN 201811258313 A CN201811258313 A CN 201811258313A CN 109158137 A CN109158137 A CN 109158137A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
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Abstract
Then the invention discloses a kind of preparation methods of micro-fluidic chip to carry out bonding preparation by the way that carbon nanomaterial is added before substrate and cover plate molding.The present invention makes carbon nanomaterial lump together with macromolecule micro-fluidic chip, bond strength is high, changes micro-fluidic chip surface microstructure and function of surface property significantly by the way that carbon nanomaterial is added in the raw material before substrate and cover plate molding.Since carbon nanomaterial has the specific surface area of very strong hydrophobicity and superelevation, when water is dispersed phase, the addition of carbon nanomaterial is easier to the formation of drop.
Description
Technical field
The present invention relates to microlayer model technical fields, and in particular to a kind of preparation work for the micro-fluidic chip for being easy to generate drop
Skill.
Background technique
Microlayer model technology is in microscale channel, by means of two kinds as an important branch of microfluidic chip technology
The essence that solution cannot dissolve each other.Using one of solution as dispersed phase, using another solution as continuous phase, in conjunction with micro-fluidic core
Sheet material, the structure in micro-fluidic chip channel and external force manipulation effect, utilize the phase between Osima jacoti, Osima excavata and surface tension
Dispersed phase is cut into a kind of micro & nano technology of the fine droplet of nanoliter level and following volumes by interaction continuous phase.
The core of microlayer model technology is also the generation that first step is microlayer model, therefore makes one kind and be conducive to generate micro- liquid
The micro-fluidic chip of drop is the key that microlayer model technology.The structure in the generation of microlayer model and micro-fluidic chip channel, hydrophilic and hydrophobic,
The properties such as specific surface area have very big relationship.
The technology for changing micro-fluidic chip channel surface lubricant nature matter at present mostly uses greatly surface coating method or is handling
Surfactant is added in target, on the one hand needs special instrument and equipment, cumbersome, cost is also higher, on the other hand
It is likely to occur the situation that coating is uneven, coating is low with micro-fluidic chip surface bonding strength.And it uses surface covering, introduce table
Face activating agent changes the method for micro-fluidic chip channel wetability, needs to introduce chemical reagent, there are problems that reagent contamination, is
Subsequent applications bring interference.
Summary of the invention
The purpose of the present invention is to solve defect existing in the prior art, provide that a kind of simple process, there is no examinations
Agent pollutes and can effectively generate the micro-flow control chip preparation method of fine droplet.
In order to achieve the above object, the present invention provides a kind of preparation methods of micro-fluidic chip, comprising the following steps:
(1) preparation of substrate: being added carbon nanomaterial in raw material liquid high molecular material and curing agent, carries out the system of substrate
It is standby；
(2) preparation of cover plate: being added carbon nanomaterial in raw material liquid high molecular material and curing agent, carries out the system of cover plate
It is standby；
(3) preparation of micro-fluidic chip: the cover plate key that will be prepared in the substrate being prepared in step (1) and step (2)
It closes up to the micro-fluidic chip；
Wherein, the carbon nanomaterial in step (1) selects one of graphene, carbon nanotube, carbon quantum dot or a variety of mixing；
Carbon nanomaterial in step (2) selects one of graphene, carbon nanotube, carbon quantum dot or a variety of mixing；Step (1) system
In the substrate obtained, the content of carbon nanomaterial is 0.005wt% ~ 0.1wt%；In cover plate made from step (2), carbon nanomaterial
Content is 0.005wt% ~ 0.1wt%.
Further, method of molding, pressure sintering, LIGA technology or laser ablation method can be used in the preparation of substrate in step (1)；
Method of molding, pressure sintering, LIGA technology or laser ablation method can be used in the preparation of step (in 2) cover plate.
The preparation of substrate and cover plate can be used method of molding and be prepared, the specific steps are as follows:
(1) preparation of substrate: taking mass ratio is the PDMS precursor and curing agent of 10:1, is stirred evenly, and carbon nanometer is added thereto
Material stirs evenly, and the substrate with microchannel is prepared in degassed, casting, solidification and demoulding；In the substrate, carbon is received
The content of rice material is 0.005wt% ~ 0.1wt%；
(2) preparation of cover plate: taking mass ratio is the PDMS precursor and curing agent of 10:1, is stirred evenly, and carbon nanometer is added thereto
Material stirs evenly, and cover plate is prepared in degassed, casting, solidification and demoulding；In the cover plate, the content of carbon nanomaterial
For 0.005wt% ~ 0.1wt%.
(3) preparation of micro-fluidic chip: the lid that will be prepared in the substrate being prepared in step (1) and step (2)
Piece is bonded up to the micro-fluidic chip.
The preparation of substrate and cover plate can be used pressure sintering and be prepared, the specific steps are as follows:
(1) preparation of substrate: taking PMMA precursor and initiator to be dissolved in DMF, and uniform PMMA/DMF solution is made, by carbon nanometer
Material is dissolved in DMF solution, obtains uniform carbon nanomaterial/DMF solution, by PMMA/DMF solution and carbon nanomaterial/DMF
It is poured into surface plate after solution mixing, it is clean to solvent volatilization, after PMMA/ carbon nanomaterial curing molding, then through hot pressing legal system
The standby substrate with microchannel；The content of carbon nanomaterial is 0.005wt% ~ 0.1wt% in the substrate；
(2) preparation of cover plate:: it takes PMMA precursor and initiator to be dissolved in DMF, uniform PMMA/DMF solution is made, carbon is received
Rice material is dissolved in DMF solution, obtains uniform carbon nanomaterial/DMF solution, by PMMA/DMF solution and carbon nanomaterial/
It is poured into surface plate after DMF solution mixing, it is clean to solvent volatilization, after PMMA/ carbon nanomaterial curing molding, it can be prepared by covering
Piece；The content of carbon nanomaterial is 0.005wt% ~ 0.1wt% in the cover plate；
(3) preparation of micro-fluidic chip: the cover plate key that will be prepared in the substrate being prepared in step (1) and step (2)
It closes up to the micro-fluidic chip.
The preparation process and raw material of substrate and cover plate choose can be different, the specific steps are as follows:
(1) preparation of substrate: taking PMMA precursor and initiator to be dissolved in DMF, and uniform PMMA/DMF solution is made, by carbon nanometer
Material is dissolved in DMF solution, obtains uniform carbon nanomaterial/DMF solution, by PMMA/DMF solution and carbon nanomaterial/DMF
It is poured into surface plate after solution mixing, it is clean to solvent volatilization, after PMMA/ carbon nanomaterial curing molding, then through hot pressing legal system
The standby substrate with microchannel；The content of carbon nanomaterial is 0.005wt% ~ 0.1wt% in the substrate；
(2) preparation of cover plate: taking mass ratio is the PDMS precursor and curing agent of 10:1, is stirred evenly, and carbon nanometer is added thereto
Material stirs evenly, and cover plate is prepared in degassed, casting, solidification and demoulding；The content of carbon nanomaterial in the cover plate
For 0.005wt% ~ 0.1wt%；
(3) preparation of micro-fluidic chip: the cover plate key that will be prepared in the substrate being prepared in step (1) and step (2)
It closes up to the micro-fluidic chip.
The present invention has the advantage that compared with prior art
1, the present invention makes carbon nanomaterial and macromolecule by the way that carbon nanomaterial is added in the raw material before substrate and cover plate molding
Micro-fluidic chip is lumped together, and bond strength is high, changes micro-fluidic chip surface microstructure and function of surface property significantly.
Since carbon nanomaterial has the specific surface area of very strong hydrophobicity and superelevation, when water is dispersed phase, carbon nanomaterial
Addition be easier to the formation of drop.
2, using the main material in high molecular polymer as micro-fluidic chip, having can be protected the present invention with method of molding height
Very replicate micro-fluidic chip, it is durable, have many advantages, such as certain chemical inertness, be easy to operate, is cheap, is nontoxic, very
It is suitable for high-volume and makes disposable micro-fluidic chip.And high molecular polymer has good optical property, in order to rear
Continuous detection application.
3, the preparation process of micro-fluidic chip of the present invention is simple, easily operated, fast and easy, greatly simplifies operation step
Suddenly, experimental period has been saved.And be not necessarily to introduce the chemical reagent such as surfactant in whole preparation process, reagent contamination is avoided,
It is environmentally protective.
Detailed description of the invention
Fig. 1 is the preparation that the modified micro-fluidic chip being prepared of carbon nanomaterial is used in the embodiment of the present invention 1 to 3
Flow chart；
Fig. 2 is the experiment effect figure that the present invention uses the modified micro-fluidic chip being prepared of carbon nanomaterial；
Fig. 3 is the experiment effect figure that the conventional microfluidic control chip that carbon nanomaterial is modified is not added.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.
Embodiment 1
As shown in Figure 1, the micro-fluidic chip preparation process that the present invention uses carbon nanomaterial modified:
(1) weigh: weighing mass ratio is the PDMS precursor and curing agent of 10:1 in beaker, is stirred evenly with glass bar.
(2) carbon nanomaterial is added: graphene is added in the beaker in step (1), makes its content 0.005wt%, stirs
It mixes uniformly.
(3) it deaerates: the beaker of step (2) being put into vacuum oven, vacuum pump is opened, the PDMS in beaker is made not overflow
Out, until the air in PDMS empties.
(4) it is poured: taking out the PDMS liquid castable of step (3) on chip formpiston, PDMS liquid flows out in order to prevent, in advance
First chip formpiston is placed in container with groove.Pay attention to being poured from centre to surrounding when casting, avoids generating bubble.
(5) solidify: the container in step (4) being put into 40 DEG C of baking oven heating 2h, takes out, sees after just having begun to warm up 10min
Bubble-free has been examined, has continued to be put into baking oven heating after having bubble to be punctured with needle.
(6) cover plate makes: according to the method for step 1 ~ 3, by the PDMS liquid castable emptied clean blank culture
On base, same to step (5) solidification.
(7) it is bonded: the substrate taken off from formpiston is faced upward with being bonded for cover plate peeled from clean culture dish, adjust
Whole Laboratory Corona Treater voltage makes to generate purple corona at electrode, apart from 4 ~ 5mm of micro-fluidic chip, handles time 1min.
And the two sides fitting of bonding, compression are put into 100 DEG C of baking ovens heating 1h to get micro-fluidic chip of the present invention in 1min clock.
Comparative example 1
Do not carry out the modified micro-fluidic chip preparation process of carbon nanomaterial using common process:
(1) weigh: weighing mass ratio is the PDMS precursor and curing agent of 10:1 in beaker, is stirred evenly with glass bar.
(2) it deaerates: the beaker of step (1) being put into vacuum oven, vacuum pump is opened, the PDMS in beaker is made not overflow
Out, until the air in PDMS empties.
(3) it is poured: taking out the PDMS liquid castable of step (2) on chip formpiston, PDMS liquid flows out in order to prevent, in advance
First chip formpiston is placed in container with groove.Pay attention to being poured from centre to surrounding when casting, avoids generating bubble.
(4) solidify: the container in step (3) being put into 40 DEG C of baking oven heating 2h, takes out, sees after just having begun to warm up 10min
Bubble-free has been examined, has continued to be put into baking oven heating after having bubble to be punctured with needle.
(5) cover plate makes: according to the method for step 1 ~ 2, by the PDMS liquid castable emptied clean blank culture
On base, same to step (4) solidification.
(6) it is bonded: the substrate taken off from formpiston is faced upward with being bonded for cover plate peeled from clean culture dish, adjust
Whole Laboratory Corona Treater voltage makes to generate purple corona at electrode, apart from 4 ~ 5mm of micro-fluidic chip, handles time 1min.
And the two sides fitting of bonding, compression are put into 100 DEG C of baking ovens heating 1h to get comparative example micro-fluidic chip in 1min clock.
Contrast on effect embodiment
What Example 1 and comparative example 1 were prepared is tested respectively, and experimental selection silicone oil is continuous phase, rhodamine B
Coloring agent is dispersed phase.The continuous phase and dispersed phase tested twice do not have any variation, and experiment is using micro-injection pump driving stream
Body enters microchannel, and the flow of each micro-injection pump is identical.The micro pump discharge for connecting branch inlet is 0.1 ~ 0.6 μ L/m, even
The micro-injection pump discharge for connecing continuous phase entrance is 1 ~ 2 μ L/m.Experimental result is the miniflow that carbon nanomaterial is added in embodiment 1
Control chip can produce single, evenly dispersed drop, and be not added in comparative example 1 micro-fluidic chip of carbon nanomaterial without
Method generates drop.Experimental result is as shown in Figure 2 and Figure 3.
According to experiment effect Fig. 2,3 it can be seen that the micro-fluidic chip preparation process that the present invention uses, effectively promotes
The generation of microlayer model, and it is easy to operate.
Comparative example 2
Using carbon nanomaterial, modified micro-fluidic chip preparation process is as follows:
(1) weigh: weighing mass ratio is the PDMS precursor and curing agent of 10:1 in beaker, is stirred evenly with glass bar.
(2) carbon nanomaterial is added: graphene is added in the beaker in step (1), makes its content 0.004wt%, stirs
It mixes uniformly.
(3) it deaerates: the beaker of step (2) being put into vacuum oven, vacuum pump is opened, the PDMS in beaker is made not overflow
Out, until the air in PDMS empties.
(4) it is poured: taking out the PDMS liquid castable of step (3) on chip formpiston, PDMS liquid flows out in order to prevent, in advance
First chip formpiston is placed in container with groove.Pay attention to being poured from centre to surrounding when casting, avoids generating bubble.
(5) solidify: the container in step (4) being put into 40 DEG C of baking oven heating 2h, takes out, sees after just having begun to warm up 10min
Bubble-free has been examined, has continued to be put into baking oven heating after having bubble to be punctured with needle.
(6) cover plate makes: according to the method for step 1 ~ 3, by the PDMS liquid castable emptied clean blank culture
On base, same to step (5) solidification.
(7) it is bonded: the substrate taken off from formpiston is faced upward with being bonded for cover plate peeled from clean culture dish, adjust
Whole Laboratory Corona Treater voltage makes to generate purple corona at electrode, apart from 4 ~ 5mm of micro-fluidic chip, handles time 1min.
And the two sides fitting of bonding, compression are put into 100 DEG C of baking ovens heating 1h to get comparative example micro-fluidic chip in 1min clock.
The micro-fluidic chip being prepared using above-mentioned steps can not generate drop, and effect in Contrast on effect embodiment the same as scheming
Shown in 3.
Comparative example 3
Using carbon nanomaterial, modified micro-fluidic chip preparation process is as follows:
(1) weigh: weighing mass ratio is the PDMS precursor and curing agent of 10:1 in beaker, is stirred evenly with glass bar.
(2) carbon nanomaterial is added: graphene is added in the beaker in step (1), makes its content 0.110wt%, stirs
It mixes uniformly.
(3) it deaerates: the beaker of step (2) being put into vacuum oven, vacuum pump is opened, the PDMS in beaker is made not overflow
Out, until the air in PDMS empties.
(4) it is poured: taking out the PDMS liquid castable of step (3) on chip formpiston, PDMS liquid flows out in order to prevent, in advance
First chip formpiston is placed in container with groove.Pay attention to being poured from centre to surrounding when casting, avoids generating bubble.
(5) solidify: the container in step (4) being put into 40 DEG C of baking oven heating 2h, takes out, sees after just having begun to warm up 10min
Bubble-free has been examined, has continued to be put into baking oven heating after having bubble to be punctured with needle.
(6) cover plate makes: according to the method for step 1 ~ 3, by the PDMS liquid castable emptied clean blank culture
On base, same to step (5) solidification.
(7) it is bonded: the substrate taken off from formpiston is faced upward with being bonded for cover plate peeled from clean culture dish, adjust
Whole Laboratory Corona Treater voltage makes to generate purple corona at electrode, apart from 4 ~ 5mm of micro-fluidic chip, handles time 1min.
And the two sides fitting of bonding, compression are put into 100 DEG C of baking ovens heating 1h to get comparative example micro-fluidic chip in 1min clock.
The micro-fluidic chip being prepared using above-mentioned steps is not easy to observe its drop due to carbon nanomaterial too high levels
It is formed, and the carbon nanomaterial of high-content increases the cost of micro-fluidic chip, it is not recommended that use.
Embodiment 2
As shown in Figure 1, the micro-fluidic chip preparation process that the present invention uses carbon nanomaterial modified:
(1) weigh: weighing mass ratio is the PDMS precursor and curing agent of 10:1 in beaker, is stirred evenly with glass bar.
(2) carbon nanomaterial is added: carbon nanotube, graphene and carbon quantum dot are added in the beaker in step (1) to be made
Its content is 0.005wt%, is stirred evenly.
(3) it deaerates: the beaker of step (2) being put into vacuum oven, vacuum pump is opened, the PDMS in beaker is made not overflow
Out, until the air in PDMS empties.
(4) it is poured: taking out the PDMS liquid castable of step (3) on chip formpiston, PDMS liquid flows out in order to prevent, in advance
First chip formpiston is placed in container with groove.Pay attention to being poured from centre to surrounding when casting, avoids generating bubble.
(5) solidify: the container in step (4) being put into 75 DEG C of baking oven heating 1h, takes out, sees after just having begun to warm up 10min
Bubble-free has been examined, has continued to be put into baking oven heating after having bubble to be punctured with needle.
(6) cover plate makes: according to the method for step 1 ~ 3, by the PDMS liquid castable emptied clean blank culture
On base, same to step (5) solidification.
(7) it is bonded: the substrate taken off from formpiston is faced upward with being bonded for cover plate peeled from clean culture dish, adjust
Whole Laboratory Corona Treater voltage makes to generate purple corona at electrode, apart from 4 ~ 5mm of micro-fluidic chip, handles time 1min.
And the two sides fitting of bonding, compression are put into 100 DEG C of baking ovens heating 1h to get micro-fluidic chip of the present invention in 1min clock.
It can produce single, evenly dispersed drop, the same effect of effect using the micro-fluidic chip that above-mentioned steps are prepared
In embodiment shown in Fig. 2.
Embodiment 3
As shown in Figure 1, the micro-fluidic chip preparation process that the present invention uses carbon nanomaterial modified:
(1) weigh: weighing mass ratio is the PDMS precursor and curing agent of 10:1 in beaker, is stirred evenly with glass bar.
(2) carbon nanomaterial is added: carbon quantum is added in the beaker in step (1), makes its content 0.1wt%, stirs
Uniformly.
(3) it deaerates: the beaker of step (2) being put into vacuum oven, vacuum pump is opened, the PDMS in beaker is made not overflow
Out, until the air in PDMS empties.
(4) it is poured: taking out the PDMS liquid castable of step (3) on chip formpiston, PDMS liquid flows out in order to prevent, in advance
First chip formpiston is placed in container with groove.Pay attention to being poured from centre to surrounding when casting, avoids generating bubble.
(5) solidify: the container in step (4) being put into 90 DEG C of baking oven heating 20min, is taken out after just having begun to warm up 10min,
Observation has bubble-free, continues to be put into baking oven heating after having bubble to be punctured with needle.
(6) cover plate makes: according to the method for step 1 ~ 3, by the PDMS liquid castable emptied clean blank culture
On base, same to step (5) solidification.
(7) it is bonded: the substrate taken off from formpiston is faced upward with being bonded for cover plate peeled from clean culture dish, adjust
Whole Laboratory Corona Treater voltage makes to generate purple corona at electrode, apart from 4 ~ 5mm of micro-fluidic chip, handles time 1min.
And the two sides fitting of bonding, compression are put into 100 DEG C of baking ovens heating 1h to get micro-fluidic chip of the present invention in 1min clock.
It can produce single, evenly dispersed drop, the same effect of effect using the micro-fluidic chip that above-mentioned steps are prepared
In embodiment shown in Fig. 2.
Embodiment 4
The present invention is as follows using the modified micro-fluidic chip preparation process of carbon nanomaterial:
(1) it weighs: weighing suitable PMMA precursor and initiator (its ratio is selected according to common process) is dissolved in two
In methylformamide (DMF), uniform PMMA/DMF solution is obtained.
(2) carbon nanomaterial is added: the graphene (GO) for accounting for raw material total amount 0.005wt% is dissolved in DMF, GO/ is made
DMF solution is added in the PMMA/DMF solution of step (1), stirs evenly, and GO/PMMA/DMF solution is made.
(3) GO/PMMA composite material solidifies: the GO/PMMA/DMF solution in step (2) is poured into surface plate, and
2d is heated on 45 DEG C of electric hot plates, obtains cured GO/PMMA composite material after DMF solution slowly volatilizees completely.
(4) substrate makes: having the PMMA substrate of microchannel using pressure sintering preparation.The specific steps are in hot-press arrangement
PMMA substrate is heated to 106 DEG C, by applying certain pressure (4 inch areas reinforce 20 ~ 30kN) on formpiston, and keeps 30
Then under an increased pressure by formpiston and substrate together cooling and demolding, the PMMA substrate with microchannel is made in ~ 60s.
(5) cover plate makes: according to the method for step 1 ~ 3, preparing GO/PMMA composite material cover plate.
(6) it is bonded: the PMMA substrate with microchannel being bonded compression with the cover plate in step (5), is placed in 105 DEG C of bakings
Case inside holding 5min can be prepared by GO/PMMA composite material micro-fluidic chip.
It can produce single, evenly dispersed drop, the same effect of effect using the micro-fluidic chip that above-mentioned steps are prepared
In embodiment shown in Fig. 2.
Embodiment 5
The present invention is as follows using the modified micro-fluidic chip preparation process of carbon nanomaterial:
(1) substrate makes: preparation has the GO/PMMA composite material substrate in channel in selection example 4.
(2) cover plate makes: the CQDS/PDMS composite material cover plate prepared in selection example 3.
(3) it is bonded: the cover plate bonding in step (5) being faced upward, regulation experiment room corona processor voltage makes at electrode
Purple corona is generated, apart from 4 ~ 5mm of micro-fluidic chip, handles time 1min.And by the cover plate after sided corona treatment in 1min clock
It is bonded, compresses with the PMMA substrate with microchannel, being put into 75 DEG C of baking oven heating 10min to get substrate is GO/PMMA composite wood
Material, cover plate are the micro-fluidic chip of CQDS/PDMS composite material.
It can produce single, evenly dispersed drop, the same effect of effect using the micro-fluidic chip that above-mentioned steps are prepared
In embodiment shown in Fig. 2.
Claims (5)
1. a kind of preparation method of micro-fluidic chip, it is characterised in that: the following steps are included:
(1) preparation of substrate: being added carbon nanomaterial in raw material liquid high molecular material and curing agent, carries out the system of substrate
It is standby；
(2) preparation of cover plate: being added carbon nanomaterial in raw material liquid high molecular material and curing agent, carries out the system of cover plate
It is standby；
(3) preparation of micro-fluidic chip: the cover plate key that will be prepared in the substrate being prepared in step (1) and step (2)
It closes up to the micro-fluidic chip；
In carbon nanomaterial selection graphene (GO), carbon nanotube (CNT), carbon quantum dot (CQDS) in the step (1)
One or more mixing；Carbon nanomaterial in the step (2) selects one of graphene, carbon nanotube, carbon quantum dot
Or a variety of mixing；In substrate made from the step (1), the content of carbon nanomaterial is 0.005wt% ~ 0.1wt%；The step
(2) in cover plate made from, the content of carbon nanomaterial is 0.005wt% ~ 0.1wt%.
2. preparation method according to claim 1, it is characterised in that: the preparation of substrate is using molding in the step (1)
Method, pressure sintering, LIGA technology or laser ablation method；The preparation of step (in the 2) cover plate uses method of molding, pressure sintering, LIGA
Technology or laser ablation method.
3. preparation method according to claim 2, it is characterised in that: the following steps are included:
(1) preparation of substrate: taking mass ratio is the PDMS precursor and curing agent of 10:1, is stirred evenly, and carbon nanometer is added thereto
Material stirs evenly, and the substrate with microchannel is prepared in degassed, casting, solidification and demoulding；Carbon is received in the substrate
The content of rice material is 0.005wt% ~ 0.1wt%；
(2) preparation of cover plate: taking mass ratio is the PDMS precursor and curing agent of 10:1, is stirred evenly, and carbon nanometer is added thereto
Material stirs evenly, and cover plate is prepared in degassed, casting, solidification and demoulding；The content of carbon nanomaterial in the cover plate
For 0.005wt% ~ 0.1wt%；
(3) preparation of micro-fluidic chip: the cover plate key that will be prepared in the substrate being prepared in step (1) and step (2)
It closes up to the micro-fluidic chip.
4. preparation method according to claim 2, it is characterised in that: the following steps are included:
(1) it the preparation of substrate: takes PMMA precursor and initiator to be dissolved in dimethylformamide (DMF), uniform PMMA/ is made
Carbon nanomaterial is dissolved in DMF solution by DMF solution, obtains uniform carbon nanomaterial/DMF solution, by PMMA/DMF solution,
It is poured into surface plate with after carbon nanomaterial/DMF solution mixing, PMMA/ carbon nanomaterial curing molding clean to solvent volatilization
Afterwards, then through pressure sintering preparation there is the substrate of microchannel；In the substrate content of carbon nanomaterial be 0.005wt% ~
0.1wt%；
(2) preparation of cover plate: taking PMMA precursor and initiator to be dissolved in DMF, and uniform PMMA/DMF solution is made, by carbon nanometer
Material is dissolved in DMF solution, obtains uniform carbon nanomaterial/DMF solution, by PMMA/DMF solution and carbon nanomaterial/DMF
It is poured into surface plate after solution mixing, it is clean to solvent volatilization, after PMMA/ carbon nanomaterial curing molding, it can be prepared by cover plate；
The content of carbon nanomaterial is 0.005wt% ~ 0.1wt% in the cover plate；
(3) preparation of micro-fluidic chip: the cover plate key that will be prepared in the substrate being prepared in step (1) and step (2)
It closes up to the micro-fluidic chip.
5. preparation method according to claim 2, it is characterised in that: the following steps are included:
(1) preparation of substrate: taking PMMA precursor and initiator to be dissolved in DMF, and uniform PMMA/DMF solution is made, carbon is received
Rice material is dissolved in DMF solution, obtains uniform carbon nanomaterial/DMF solution, by PMMA/DMF solution and carbon nanomaterial/
It is poured into surface plate after DMF solution mixing, it is clean to solvent volatilization, after PMMA/ carbon nanomaterial curing molding, then through pressure sintering
Prepare the substrate with microchannel；The content of carbon nanomaterial is 0.005wt% ~ 0.1wt% in the substrate；
(2) preparation of cover plate: taking mass ratio is the PDMS precursor and curing agent of 10:1, is stirred evenly, and carbon nanometer is added thereto
Material stirs evenly, and cover plate is prepared in degassed, casting, solidification and demoulding；The carbon nanomaterial accounts for PDMS precursor, consolidates
0.005wt% ~ 0.1wt% of agent and carbon nanomaterial total amount；
(3) preparation of micro-fluidic chip: the cover plate key that will be prepared in the substrate being prepared in step (1) and step (2)
It closes up to the micro-fluidic chip.
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| CN111292920A (en) * | 2019-12-30 | 2020-06-16 | 南京理工大学 | Super-hydrophobic magnetic response micro-rib array for directionally transporting liquid drops and preparation method thereof |
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| CN110108552B (en) * | 2019-06-17 | 2024-03-08 | 南京工业大学 | Detection method and detection device for flexible force-induced color change material |
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