CN109134591A - A kind of balsam pear peptide extracting method and its application - Google Patents
A kind of balsam pear peptide extracting method and its application Download PDFInfo
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- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
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- C07K1/14—Extraction; Separation; Purification
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Abstract
The present invention provides a kind of plant polypeptide extracting method and its application, more particularly to a kind of bitter gourd polypeptide extracting method, this method successively uses miillpore filter, rolling ultrafiltration membrane to carry out clarification to balsam pear Aqueous extracts, trapped fluid after purification using concentration, dialyse and be drying to obtain bitter gourd polypeptide, bitter gourd polypeptide content prepared by the present invention is up to 30% or more, impurity-eliminating effect is obvious, can significantly improve it in the curative effect of hypoglycemic aspect.
Description
Technical field
The invention belongs to field of biotechnology, it is related to a kind of plant active peptides extracting method and its application, and in particular to one
Kind balsam pear peptide extracting method and its application.
Background technique
In recent years, diabetes have become one of the 3 big Chronic Non-Communicable Diseases that the whole world threatens human health.International glycosuria
Sick alliance the 5th edition points out that diabetic is more than 92,400,000 in Chinese 20-79 years old crowd, and it is total to account for about global diabetic
Several 1/4, diabetes have become a big killer of current threat human health.6 diabetes epidemic diseases are successively carried out in China
Learn investigation: the 300000 population diabetes investigation carried out for 1980 finds that its illness rate is only 0.67%；It is related to 19 provinces and cities within 1994
2nd diabetes of about 210,000 populations are generally investigated, and discovery illness rate rises to 2.28%；Diabetes to 11 provinces and cities' populations in 1996
Sample investigation shows that illness rate is 3.62%；Investigation in 2002 finds that China city diabetes prevalence is about 4.5%；2010
The diabetes investigation of year 18 years old above more than 90,000 population of national 31 provinces and cities shows that diabetic leads and has been up to 9.65%.With people
Improvement of living standard, nutriment intake increases, work rhythm is accelerated, diabetes prevalence rises year by year, development trend
It allows of no optimist.
Currently, the treatment of diabetes, chemical drug mainly uses insulin injection, sulfonylurea drugs and biguanides to be combined
Deng though effect is good, side effect is more, normally behaves as: hypoglycemic reaction, local subcutaneous lipoatrophia or hyperplasia, insulin mistake
Quick, insulin edema, ametropia, weight gain, damage liver kidney etc..These side effects bring great pain to diabetic
It is bitter.
Balsam pear also known as balsam pear belong to cucurbitaceous plant, are annual scandent herb, and fruit is oblong, are Huang when mature
Color, surface have most irregular wart like structures, and seed is hidden among Fleshy fruits.Cure mainly heatstroke, hot summer weather polydipsia, heat furuncle, miliaria
The illnesss such as sub excessive, dysentery, sore swells, conjunctivitis, red eye, swell pain, carbuncle swells erysipelas, burn and scald, oliguresis.In addition, the vitamin of balsam pear
C content is very high, has prevention scurvy, protection cell membrane, atherosclerosis, improves physical stress ability, the protection heart
A kind of ingredient is contained in the effects of dirty balsam pear the inside, can effectively inhibit the canceration of normal cell and promote the recovery of the special born of the same parents that attenuate,
With certain antitumaous effect.In addition, containing trypsin inhibitor in balsam pear, the secreted albumen come out of cancer cell can inhibit
Enzyme prevents malignant tumour, but also has reducing blood lipid and the effect of blood pressure.Khana in 1981 is reported by balsam pear fruit, kind
The ingredient that molecular weight is 11kDa is extracted in son and tissue, which has human body I, type II diabetes good
Good hypoglycemic activity, but oral meeting be destroyed in enteron aisle and be inactivated.
Patent application CN101985644A discloses a kind of bitter gourd polypeptide preparation method, and freeze-drying balsam pear powder is directly digested about
Obtain the mixed liquor containing bitter gourd polypeptide within 8 hours, further refrigeration sedimentation 48-72 hours, and handle 30 points in 100 DEG C of enzyme-deactivatings
Clock.Patent application CN104605136A also discloses a kind of preparation method of bitter gourd polypeptide, by bitter melon seed powder after deoiling, benefit
The method separation bitter gourd polypeptide of filtering, then acid adding sedimentation is extracted with buck, extraction and sedimentation time are respectively 24 hours.Above-mentioned side
It sinks using all kinds of enzyme digestion reactions, alkali carries acid in method or the production methods such as other sedimentations is combined to obtain balsam pear peptide, preparation time period
Long and certain steps such as enzyme deactivation operation temperature is high, causes product to inactivate, efficiency is extremely low, and high production cost is brought for many patients
Great financial burden.The bitter gourd polypeptide of natural activity is the key component of Effective Regulation metabolism of blood glucose, therefore, is badly in need of a kind of energy
Effective production method of the active bitter gourd polypeptide protein extract of enough extraction separation and purification high levels.
Traditional Chinese medicine has accumulated rich experiences to the treatment of diabetes.Diabetes, " more drinks eats diuresis to Huangdi's Internal Classics
And weight loss " typical three-many-one-little be attributed to " diabete " scope.Basic Theories of Chinese Medicine thinks, eating and drinking without temperance cause taste by
Damage, essence of water and grain transporting are obstructed, and water-wet stops gathering, and the raw phlegm of wine is turbid, breed phlegm wet and interior heat, and retention of phlegm-heat in the interior hair is to quench one's thirst.A variety of medicines
Material, Multiple components combo Chinese medicine are used for the treatment of diabete, many classics under the theoretical direction of monarch and diagnosis and treatment
Chinese medicinal formulae was passed on after practice test in thousand.
However there are many Chinese patent drugs for treatment diabetes on the market at present, but it works, slow, therapeutic effect is barely satisfactory, and
Animal pancreatic is a regulation body function carbohydrate metabolism and the treasure-house for generating a large amount of active biomolecules, have catalytic proteins,
Fat, starch hydrolysis, have aid digestion, and pancreatic lipase has control rouge dysentery effect even if low dose, and pig pancreas can enhance rouge
Albumen lipase activity repairs impaired pancreas, excreting insulin is promoted to play most important effect to blood glucose is reduced.Patent application
CN104056164A discloses a kind of Chinese medicine composition for treating diabetes, by fresh pig pancreas, the larva of a silkworm with batrytis, leech, endothelium corneum gigeriae galli, west
American ginseng, Radix Notoginseng, balsam pear, fructus schisandrae, lance asiabell root, fructus amomi, walnut kernel, fructus lycii, rhizoma atractylodis, black soybean, hawthorn, Radix Astragali, wild agaric,
Pueraria lobata, Chinese yam composition, the composition is rich in numerous nutrition such as amino acid abundant, microelement, cellulose, flavone compounds
Ingredient, is capable of the release of insulin, while promoting gastric emptying, inhibits the absorption of sugar and fat, realizes the treatment of diabetes.Patent
Application CN1833706A discloses a kind of drug for treating diabetes comprising radix pseudostellariae, Schisandra chinensis, raw Radix Astragali, RHIZOMA POLYGONATI PREPARATA, Chinese holly
Matrimony vine, Poria cocos, Chinese yam, Radix Rehmanniae, radix polygonati officinalis, Radix Ophiopogonis can also add animal pancreas extract, and it is invalid that the composition controls Western medicine long
The gerontal patient of diabetes and its concurrent peripheral neuropathy treats 13, and through treatment in 4-5 months, symptom, sign and surrounding were transported
Dynamic nerve conduction velocity has improvement.But pig pancreas and Chinese medicine are only combined compatibility in above-mentioned patent, in pig pancreas
The chief active factor is suppressed, it is difficult to be played one's part to the full, final therapeutic effect is still limited.
To reduce the disease to massive losses caused by society, the puzzlement of solution many patients, patients ' life quality is improved, is ground
The hypoglycemic medicine for sending out hypoglycemic good effect Small side effects a kind of is very urgent.
Summary of the invention
Based on the defect of the above-mentioned prior art, the present invention provides a kind of extracting method of balsam pear peptide, and this method avoids digesting
Reaction, alkali carries acid is heavy or combines the production methods such as other sedimentations, and impurity in balsam pear is only removed by way of multistage filtering, is prepared
Obtained balsam pear peptide content is up to 30% or more.
The present invention provides a kind of extracting method of bitter gourd polypeptide, comprising the following steps:
(1) balsam pear cleaning, mashing are broken, and extracting in water, centrifugation obtain centrifugate；
(2) centrifugate is successively passed through to microfiltration membrane system, rolling ultrafiltration membrane purification system, obtains ultrafiltration membrane trapped fluid
(3) ultrafiltration membrane trapped fluid is concentrated, obtains concentrate；
(4) concentrate is dried, obtains bitter gourd polypeptide.
Further, the molecular cut off of miillpore filter system is 5 μm -50 μm in the step (2)；The roll-to-roll ultrafiltration
Film purification system using two-stage retain, respectively molecular cut off be 150kDa and 800Da rolling ultrafiltration membrane purification system.
Further, it is concentrated in the step (3) using rolling high pressure reverse osmosis membrane system, molecular cut off >=
100Da。
The present invention also provides the bitter gourd polypeptides that above-mentioned preparation method is prepared.
Further, in the bitter gourd polypeptide being prepared content 30% or more.
The present invention also provides application of the above-mentioned bitter gourd polypeptide in terms of hypoglycemic drug is treated in preparation.
Based on the defect of the above-mentioned prior art, the present invention is made by the collaboration between vegetable-derived components and animal source component
With, after taking can rapid hypoglycemic, the especially addition of balsam pear peptide, on the one hand have activated the activity of insulin gene in pig pancreas,
Play the role of protecting patient's pancreas, another aspect balsam pear peptide and remaining vegetable extract reduce blood glucose level in patients jointly, right
Insulin-dependent diabetes mellitus and Non-Insulin Dependent Diabetes Mellitus have a better effect, and completely alternative insulin uses,
It avoids and directly uses insulin bring toxic side effect.
The present invention provides a kind of hypoglycemic medicine composition, by Radix Astragali, Chinese yam, Radix Codonopsis, fructus lycii, Poria cocos, corn stigma,
Peach kernel, pig pancreas and balsam pear peptide composition.
Further, the composition is grouped as by following portions by weight group: 8-12 parts of Radix Astragali, 9-15 parts of Chinese yam, Radix Codonopsis
5-11 parts, 6-12 parts of fructus lycii, 3-8 parts of Poria cocos, 10-15 parts of corn stigma, 5-10 parts of peach kernel, 10-15 parts of pig pancreas, balsam pear peptide 5-
10 parts.
Further, the composition is grouped as by following portions by weight group: 9-11 parts of Radix Astragali, 10-13 parts of Chinese yam, party
Join 7-10 parts, 8-10 parts of fructus lycii, 5-6 parts of Poria cocos, 12-14 parts of corn stigma, 6-8 parts of peach kernel, 12-14 parts of pig pancreas, balsam pear peptide
6-8 parts.
Further, the composition is grouped as by following portions by weight group: 10 parts of Radix Astragali, 12 parts of Chinese yam, Radix Codonopsis 8
Part, 9 parts of fructus lycii, 5 parts of Poria cocos, 13 parts of corn stigma, 7 parts of peach kernel, 13 parts of pig pancreas, 7 parts of balsam pear peptide.
The present invention also provides a kind of drugs for reducing blood glucose, by above-mentioned composition and pharmaceutically acceptable auxiliary material group
At.
Further, the dosage form of the hypoglycemic drug is one of particle, tablet, capsule.
The present invention further provides the preparation methods of above-mentioned composition, comprising the following steps:
(1) by Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel extracting in water, it is spare to obtain mixture 1 for concentration；
(2) Chinese yam, Poria cocos are crushed, it is spare obtains mixture 2；
(3) pig pancreas is subjected to freeze-drying crushing, it is spare obtains pig pancreas powder；
(4) by balsam pear Gly-His-Lys and mixture 1, mixture 2, pig pancreas powder mix to get.
Further, enzyme preparation enzymatic hydrolysis is first added in the step (1), adds 50-60 DEG C of extraction 2-3h of water.
Further, the enzyme preparation is the mixture of cellulase and proteolytic enzyme.
Further, the mass ratio of the cellulase and proteolytic enzyme is 1-3:1.
Further, the mass ratio of the cellulase and proteolytic enzyme is 2:1.
Further, after Chinese yam, Poria cocos crush in the step (2), 60-100 mesh is crossed.
Further, pig pancreas removes adipose tissue, is cleaned with physiological saline in the step (3), smashs to pieces, and acetic acid is added
Impregnate 15-20h, be freeze-dried, crush it is spare.
Further, balsam pear Gly-His-Lys, mixture 1, mixture 2 are prepared as including by beta-cyclodextrin in the step (4)
Object, then mixed with pig pancreas powder.
The present invention further provides application of the above-mentioned composition in preparation treatment hypoglycemic drug.
Further, the composition preparation treatment insulin-dependent diabetes mellitus and Non-Insulin Dependent Diabetes Mellitus medicine
Application in object.
The invention has the benefit that
(1) vegetable-derived components and animal source component are used cooperatively by the present invention, play synergistic effect between the two, effectively
It is improved in the effect of hypoglycemic aspect.The especially addition of bitter gourd polypeptide, is on the one hand able to enter inside porcine pancreatic cell, promotees
Into the release of active factors, such as trypsase；On the other hand, bitter gourd polypeptide also can enter patient's islet cells, help to repair
Islet cell function realizes hypoglycemic effect；Finally, bitter gourd polypeptide itself has structure similar with insulin, has and adjust
The effect of blood glucose.
(2) the plant extracts Radix Astragali in composition of the invention, Chinese yam, Radix Codonopsis, fructus lycii, Poria cocos, corn stigma, peach kernel
Common Traditional Chinese medicine for reducing blood glucose ingredient, but the present invention is by reasonably combining, and by its creatively with pig pancreas and balsam pear
Polypeptides in combination application further promotes plant extracts to play a role.
(3) present composition uses Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel enzymatic hydrolysis water to mention during the preparation process,
And the mode that Chinese yam, Poria cocos directly crush, it is Radix Astragali, Radix Codonopsis, fructus lycii, corn by constantly studying and exploration discovery
Palpus, peach kernel extract again after enzyme preparation hydrolysis is added, and can destroy fibre structure and protein therein, realize effective component
Efficiently concentrating；And active constituent type is more in Chinese yam, Poria cocos, and oral absorption is high-efficient, mentioned by water or alcohol extracting instead
The content of active constituent can be reduced.
(4) bitter gourd polypeptide and Chinese medical extract and herb powder can be formed inclusion by beta-cyclodextrin inclusion compound by the present invention
Object avoids bitter gourd polypeptide and plant component from directly contacting with pig pancreas during storage and active factors is gradually caused to inactivate,
After inclusion, each ingredient enters gradually to be discharged in vivo, and bitter gourd polypeptide enters inside porcine pancreatic cell, further promote it is active because
The release of son, guarantees the activity of active factors.
Specific embodiment
Embodiment 1
Hypoglycemic medicine composition composition: 10 parts of Radix Astragali, 12 parts of Chinese yam, 8 parts of Radix Codonopsis, 9 parts of fructus lycii, 5 parts of Poria cocos, corn stigma
13 parts, 7 parts of peach kernel, 13 parts of pig pancreas, 7 parts of balsam pear peptide.
The preparation method of the hypoglycemic medicine composition, comprising the following steps:
(1) enzyme preparation enzymatic hydrolysis is added in Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel, adds water in 50 DEG C of extraction 3h, subtracts
Pressure concentration, obtains mixture 1, spare；
(2) Chinese yam, Poria cocos are crushed, crosses 80 meshes, obtains mixture 2, it is spare；
(3) fresh pig pancreas is subtracted into superabundant fats tissue, after being cleaned with physiological saline, smashed to pieces, acetic acid is added and impregnates
Then 15h carries out freeze-drying crushing, obtain pig pancreas powder, spare；
(4) by balsam pear Gly-His-Lys and mixture 1, mixture 2 and beta-cyclodextrin mixed grinding, it is prepared into inclusion compound, then again
With pig pancreas powder mix to get.
It is 2:1 that wherein the enzyme preparation, which is the mass ratio of cellulase and proteolytic enzyme,.
Embodiment 2
Hypoglycemic medicine composition composition: 12 parts of Radix Astragali, 15 parts of Chinese yam, 11 parts of Radix Codonopsis, 12 parts of fructus lycii, 6 parts of Poria cocos, corn
It must 14 parts, 8 parts of peach kernel, 14 parts of pig pancreas, 8 parts of balsam pear peptide.
The preparation method of the hypoglycemic medicine composition, comprising the following steps:
(1) enzyme preparation enzymatic hydrolysis is added in Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel, adds water in 55 DEG C of extraction 3h, subtracts
Pressure concentration, obtains mixture 1, spare；
(2) Chinese yam, Poria cocos are crushed, is sieved with 100 mesh sieve, obtain mixture 2, it is spare；
(3) fresh pig pancreas is subtracted into superabundant fats tissue, after being cleaned with physiological saline, smashed to pieces, acetic acid is added and impregnates
Then 17h carries out freeze-drying crushing, obtain pig pancreas powder, spare；
(4) by balsam pear Gly-His-Lys and mixture 1, mixture 2 and beta-cyclodextrin mixed grinding, it is prepared into inclusion compound, then again
With pig pancreas powder mix to get.
It is 2:1 that wherein the enzyme preparation, which is the mass ratio of cellulase and proteolytic enzyme,.
Embodiment 3
Hypoglycemic medicine composition composition: 9 parts of Radix Astragali, 10 parts of Chinese yam, 7 parts of Radix Codonopsis, 8 parts of fructus lycii, 3 parts of Poria cocos, corn stigma
10 parts, 5 parts of peach kernel, 10 parts of pig pancreas, 5 parts of balsam pear peptide.
The preparation method of the hypoglycemic medicine composition, comprising the following steps:
(1) enzyme preparation enzymatic hydrolysis is added in Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel, adds water in 50 DEG C of extraction 2h, subtracts
Pressure concentration, obtains mixture 1, spare；
(2) Chinese yam, Poria cocos are crushed, crosses 80 meshes, obtains mixture 2, it is spare；
(3) fresh pig pancreas is subtracted into superabundant fats tissue, after being cleaned with physiological saline, smashed to pieces, acetic acid is added and impregnates
Then 18h carries out freeze-drying crushing, obtain pig pancreas powder, spare；
(4) by balsam pear Gly-His-Lys and mixture 1, mixture 2 and beta-cyclodextrin mixed grinding, it is prepared into inclusion compound, then again
With pig pancreas powder mix to get.
It is 1:1 that wherein the enzyme preparation, which is the mass ratio of cellulase and proteolytic enzyme,.
Embodiment 4
Hypoglycemic medicine composition composition: 11 parts of Radix Astragali, 13 parts of Chinese yam, 10 parts of Radix Codonopsis, 10 parts of fructus lycii, 8 parts of Poria cocos, corn
It must 15 parts, 10 parts of peach kernel, 15 parts of pig pancreas, 10 parts of balsam pear peptide.
The preparation method of the hypoglycemic medicine composition, comprising the following steps:
(1) add water in 50 DEG C of extraction 3h in Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel, be concentrated under reduced pressure, obtain mixture 1,
It is spare；
(2) Chinese yam, Poria cocos are crushed, crosses 80 meshes, obtains mixture 2, it is spare；
(3) fresh pig pancreas is subtracted into superabundant fats tissue, after being cleaned with physiological saline, smashed to pieces, carry out freeze-drying crushing,
Pig pancreas powder is obtained, it is spare；
(4) by balsam pear Gly-His-Lys and mixture 1, mixture 2 and pig pancreas powder mix to get.
It is 3:1 that wherein the enzyme preparation, which is the mass ratio of cellulase and proteolytic enzyme,.
Embodiment 5
Hypoglycemic medicine composition composition: 8 parts of Radix Astragali, 9 parts of Chinese yam, 5 parts of Radix Codonopsis, 6 parts of fructus lycii, 3 parts of Poria cocos, corn stigma 12
Part, 6 parts of peach kernel, 11 parts of pig pancreas, 6 parts of balsam pear peptide.
The preparation method of the hypoglycemic medicine composition, comprising the following steps:
(1) enzyme preparation enzymatic hydrolysis is added in Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel, adds water in 60 DEG C of extraction 2h, subtracts
Pressure concentration, obtains mixture 1, spare；
(2) Chinese yam, Poria cocos are crushed, crosses 60 meshes, obtains mixture 2, it is spare；
(3) fresh pig pancreas is subtracted into superabundant fats tissue, after being cleaned with physiological saline, smashed to pieces, acetic acid is added and impregnates
Then 20h carries out freeze-drying crushing, obtain pig pancreas powder, spare；
(4) by balsam pear Gly-His-Lys and mixture 1, mixture 2 and beta-cyclodextrin mixed grinding, it is prepared into inclusion compound, then again
With pig pancreas powder mix to get.
It is 2:1 that wherein the enzyme preparation, which is the mass ratio of cellulase and proteolytic enzyme,.
Comparative example 1 does not contain the hypoglycemic medicine composition and preparation method thereof of bitter gourd polypeptide
Except without containing in addition to bitter gourd polypeptide, remaining with embodiment 1, it is specific the preparation method comprises the following steps:
(1) enzyme preparation enzymatic hydrolysis is added in Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel, adds water in 50 DEG C of extraction 3h, subtracts
Pressure concentration, obtains mixture 1, spare；
(2) Chinese yam, Poria cocos are crushed, crosses 80 meshes, obtains mixture 2, it is spare；
(3) fresh pig pancreas is subtracted into superabundant fats tissue, after being cleaned with physiological saline, smashed to pieces, acetic acid is added and impregnates
Then 15h carries out freeze-drying crushing, obtain pig pancreas powder, spare；
(4) by mixture 1, mixture 2 and beta-cyclodextrin mixed grinding, be prepared into inclusion compound, then again with pig pancreas powder
End mix to get.
Comparative example 2 does not contain the hypoglycemic medicine composition and preparation method thereof of pig pancreas
Except without containing in addition to pig pancreas, remaining with embodiment 1, it is specific the preparation method comprises the following steps:
(1) enzyme preparation enzymatic hydrolysis is added in Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel, adds water in 50 DEG C of extraction 3h, subtracts
Pressure concentration, obtains mixture 1, spare；
(2) Chinese yam, Poria cocos are crushed, crosses 80 meshes, obtains mixture 2, it is spare；
(3) by balsam pear Gly-His-Lys and mixture 1, mixture 2 and beta-cyclodextrin mixed grinding mix to get.
Comparative example 3
In addition to replacing corn stigma with rhizoma alismatis, remaining is the same as embodiment 1.
Comparative example 4
In addition to replacing Radix Astragali with radix saposhnikoviae, remaining is the same as embodiment 1.
1 different pharmaceutical composition hypoglycemic activity of experimental example
1.1 test specimen
The hypoglycemic medicine composition of embodiment 1-5, comparative example 1-4 preparation
1.2 experimental animal
Healthy SD mouse (cleaning grade), half male and half female, weight (20g-30g).
The foundation and grouping of 1.3 diabetic mouse models
Mouse 120 are taken, half male and half female randomly selects 10 and is only used as blank control group, remaining mouse feeding high glucose and high fat
Feed one week, then the alloxan solution 65mg/kg of the fresh configuration of tail vein injections, with blood sugar kit pair behind third day
The mouse of fasting 12h surveys its fasting blood-glucose, chooses mouse of the blood glucose higher than 11mmol/l as experimental subjects, randomly selects qualification
Mouse is divided into 10 groups, if diabetic model group, embodiment 1-5 group, and comparative example 1-4 group.
1.4 hypoglycemic experimental methods
Blank control group and the isometric physiological saline of model group stomach-filling, embodiment 1-5, comparative example 1-4 are respectively with 40mg/
The dosage stomach-filling of kg, continuous 6 weeks, respectively at the 2nd week, the 4th week, the 6th week survey fasting blood sugar.
1.5 statistical method
Indices indicate with x ± s, the t method of inspection of the significant difference between mean
The analysis of 1.6 oral solution hypoglycemic effects
Influence of 1 different components of table to mouse blood sugar
Note: respectively arranging to represent between different letters has significant difference (P < 0.05)
As seen from the above table, blood glucose has aobvious without significant changes, model group with blank control group blood glucose after naive mice stomach-filling
It writes difference (P < 0.05), illustrates model foundation success, embodiment 1-5 and comparative example 1-4 each group have significantly with model group blood glucose
Difference (P < 0.05), wherein the blood sugar decreasing effect of embodiment 1 is best, and embodiment 4 uses enzyme preparation due to no in preparation process,
Also inclusion compound being prepared without addition beta-cyclodextrin, causes plant component extraction incomplete, effective component is destroyed in pig pancreas,
Its effect for reducing blood glucose is relatively poor.Comparative example 1 and comparative example 2 lack balsam pear peptide and pig pancreas, hypoglycemic effect respectively
Fruit is decreased obviously, and Chinese medicine similar in a kind of function is substituted in comparative example 3 and 4 respectively, and curative effect is significantly reduced.Thus may be used
See, hypoglycemic medicine composition provided by the invention has significant blood sugar reducing function, cooperates with and plays a role between each component, realizes good
Good hypoglycemic curative effect.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, the embodiment not to
The scope of the patents of the invention is limited, all equivalence enforcements or change without departing from carried out by the present invention are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (10)
1. a kind of extracting method of bitter gourd polypeptide, which comprises the following steps:
(1) balsam pear cleaning, mashing are broken, and extracting in water, centrifugation obtain centrifugate；
(2) centrifugate is successively passed through to microfiltration membrane system, rolling ultrafiltration membrane purification system, obtains ultrafiltration membrane trapped fluid
(3) ultrafiltration membrane trapped fluid is concentrated, obtains concentrate；
(4) concentrate is placed in bag filter after dialysing and is dried, obtain bitter gourd polypeptide.
2. a kind of bitter gourd polypeptide that the extracting method of bitter gourd polypeptide described in claim 1 is prepared.
3. application of the bitter gourd polypeptide as claimed in claim 2 in terms of preparing hypoglycemic drug.
4. a kind of hypoglycemic medicine composition, which is characterized in that the composition by Radix Astragali, Chinese yam, Radix Codonopsis, fructus lycii, Poria cocos,
Corn stigma, peach kernel, pig pancreas and balsam pear peptide composition.
5. hypoglycemic medicine composition according to claim 4, which is characterized in that the composition is by following portions by weight
Group is grouped as: 8-12 parts of Radix Astragali, 9-15 parts of Chinese yam, 5-11 parts of Radix Codonopsis, 6-12 parts of fructus lycii, 3-8 parts of Poria cocos, corn stigma 10-15
Part, 5-10 parts of peach kernel, 10-15 parts of pig pancreas, 5-10 parts of balsam pear peptide；Preferably, the composition is by following portions by weight component
Composition: 9-11 parts of Radix Astragali, 10-13 parts of Chinese yam, 7-10 parts of Radix Codonopsis, 8-10 parts of fructus lycii, 5-6 parts of Poria cocos, 12-14 parts of corn stigma,
6-8 parts of peach kernel, 12-14 parts of pig pancreas, 6-8 parts of balsam pear peptide；It is highly preferred that the composition is grouped by following portions by weight group
At: 10 parts of Radix Astragali, 12 parts of Chinese yam, 8 parts of Radix Codonopsis, 9 parts of fructus lycii, 5 parts of Poria cocos, 13 parts of corn stigma, 7 parts of peach kernel, 13 parts of pig pancreas,
7 parts of balsam pear peptide.
6. a kind of drug for reducing blood glucose, which is characterized in that the drug is by composition described in claim 4 or 5 and medicine
Auxiliary material composition is subjected on.
7. a kind of preparation method of composition described in claim 4 or 5, comprising the following steps:
(1) by Radix Astragali, Radix Codonopsis, fructus lycii, corn stigma, peach kernel extracting in water, it is spare to obtain mixture 1 for concentration；
(2) Chinese yam, Poria cocos are crushed, it is spare obtains mixture 2；
(3) pig pancreas is subjected to freeze-drying crushing, it is spare obtains pig pancreas powder；
(4) by balsam pear Gly-His-Lys and mixture 1, mixture 2, pig pancreas powder mix to get.
8. preparation method according to claim 7, which is characterized in that enzyme preparation enzymatic hydrolysis is first added in step (1), adds
50-60 DEG C of extraction 2-3h of water.
9. preparation method according to claim 8, which is characterized in that the enzyme preparation is cellulase and proteolytic enzyme
Mixture；Preferably, the mass ratio of the cellulase and proteolytic enzyme is 1-3:1；It is highly preferred that the cellulase
Mass ratio with proteolytic enzyme is 2:1.
10. application of the composition of claim 4 or 5 in preparation treatment hypoglycemic drug.
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