CN109106942B - It is a kind of to pass through the application of the polypeptide of blood-brain barrier in medicine preparation - Google Patents
It is a kind of to pass through the application of the polypeptide of blood-brain barrier in medicine preparation Download PDFInfo
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Abstract
The present invention be it is a kind of can be by the application of the polypeptide of blood-brain barrier in medicine preparation.The present invention is attached with the N-terminal of the C-terminal of ziconotide and penetratin by 1 glycine, and one kind of acquisition can pass through the polypeptide of blood-brain barrier.Polypeptide of the invention is suitable for vein, abdominal cavity or nasal-cavity administration mode, and easy to operate, clinical risk is small, and by vein, abdominal cavity or nasal administration, the drug action time in vivo is long, and analgesic effect is excellent, peptide Small side effects, is suitable for large-scale clinical application.Polypeptide preparation of the invention is simple, quality controllable in preparation process and preparation process, is suitable for large-scale industrialized production.
Description
Technical field
The invention belongs to polypeptide drugs technical field, in particular to a kind of fused polypeptide of ziconotide is in medicine preparation
Application.
Background technique
Ziconotide (trade name PrialtTM, Elan Pharmaceuticals) is U.S.'s food officina (FDA) 2004
The first conotoxin class drug of approval.Its targeting site is N-type voltage-gated calcium channel, is cavum subarachnoidale
(intrathecal) compound analgesic first-line drug.Ziconotide is hydrophilic in the internal venom peptide of Pacific Ocean ichthyophagy spiral shell --- cone shell ---
Property polypeptide ω-MVIIA synthetic material, be it is first be applied to clinical novel non-m orphine analgestic, molecular formula is
C102H172N36O32S7, structural formula:
H-Cys-Lys-Gly-Lys-Gly-Ala-Lys-Cys-Ser-Arg-Leu-Met-Tyr-Asp-Cys-Cys-
Thr-Gly-Ser-Cys-Arg-Ser-Gly-Lys-Cys-NH2。
Ziconotide clinic can treat post-herpetic neuralgia, phantom limb pain, AIDS related neuropathy rationality pain
Bitterly, intractable pain caused by cancer, postoperative pain are not resistant to or refuse other treatment method such as systemic analgesia medicine, adjuvant treatment, delay
Solve the invalid pain etc. of intrathecal injection opioid drug.With salicylate, NSAIDs and local anesthetic mainly pass through periphery mind
Through/nociceptor, it is different with consciousness to eliminate pain that opioid drug and general anesthetic mainly act on cerebral levels, together
The mechanisms of therapeutic action for examining promise peptide is that the ability of N-type calcium channel receptor is combined by it.N-type calcium channel receptor is located at spinal cord
On main nocuity A- δ and C- slowfiber pain fiber (nociceptor) in Rexed'sI and II layers of posterior face, it can delay
Solve other treatment means include the invalid pain of intrathecal morphine, and for a long time using the drug will not generate tolerance and at
Addiction, applicating finger syndrome are to treat chronic ache relevant to wound, tumour and neuralgia etc., especially in treatment to opioid drug
Insensitive intractable pain has original advantage to opiates intolerable patient aspect.However, ziconotide cannot
Across blood-brain barrier, intrathecal intravasal administration approach is used only at present and is transfused, intubation is buried with infusion pump in subcutaneous, needs to have performed the operation
At clinical use is inconvenient.It is currently only used for long-term, the permanent treatment to existing analgesic drug product resistance type, chronic ache.This
Kind administration mode strongly limits the clinical application of the medicine intrinsic advantages.
Blood-brain barrier (BBB) is a complex cell system being present between brain tissue and blood, can control blood brain two
The substance transportation of side, so that the utility for guaranteeing that nervous centralis is knitted in the stabilization blood of interior environment passes through microvascular endothelial cells
Various acceptor interactions on film, and need to be transported into brain by body, to play a role；And it is some toxic
Harmful substance is then shielded from outside brain tissue by the barrier, to prevent generating damage to brain.This special guarantor of blood-brain barrier (BBB)
Shield effect, results in most of drug and is difficult to enter brain, the treatment and administration of this nervous centralis class disease cause to perplex.
Cell-penetrating peptides (cell penetrating peptides, CPP) are that one kind can enter cell by biomembrane
Small peptide (being generally less than 35 amino acid residues).It finds to be derived from 1988, has scholar to find the trans-activator of HIV-1
Tat can spanning transduction membrane to intracellular, and it has been found that drosophila transcription factor also has similar characteristics.Hereafter, other many CPP
It is found successively, CPP shows diversity, contained amino in relative molecular mass, amino acid composition, amino acid range sequence
Sour value volume and range of product is different, and polarity and electrically charged amount are also different, but they have the characteristics that it is some common, such as: lower
Concentration conditions under, can pass through cell membrane enter cell and film will not be caused obviously destroy and damage；Itself have and wears
Permeable membrane ability, and can also mediate various substances includes that small molecule, nucleic acid, polypeptide and nanoparticle etc. enter born of the same parents；Efficiently,
Low toxicity.In recent years, it the study found that being connect using cell-penetrating peptides with drug molecule, can achieve across blood-brain barrier effect, this
New direction is brought to nervous centralis administration.
Currently, the technical method that blood-brain barrier is passed through using cell-penetrating peptides auxiliary conotoxin exists in the prior art,
For example, ziconotide is packaged in virion, and TAT polypeptide is connected in surfaces of viral particles, which can pass
Send ziconotide by blood-brain barrier, still, the complex process of such method preparation, viral packaging process can not carry out
Good quality control, it is difficult to industrially large-scale application.For another example, the N-terminal of tat peptide and conotoxin is made by two GG
For connection unit, fused polypeptide is prepared, which can be suitable for intravenous injection administration by blood-brain barrier, but
It is that the fused polypeptide of N-terminal connection is by intravenous injection administration, analgesic effect in vivo and there are the times to be all unable to reach clinic
The requirement of application can not be promoted on a large scale.Therefore, obtain one kind can by blood-brain barrier, overcome sheath interpolation pipe to
The shortcomings that medicine, and can the modified ziconotide of clinically large-scale use be current urgent problem.
Summary of the invention
One of the object of the invention is to provide one kind can be by the polypeptide of blood-brain barrier.The present inventor passes through the hair that studies for a long period of time
It is existing, connected by the C-terminal of ziconotide and the N-terminal of penetratin, obtain can by the fusogenic peptide of blood-brain barrier, can with gram
Take the defect of the prior art, be suitable for vein, abdominal cavity or nasal-cavity administration, and analgesic effect in vivo is good and efficacy time compared with
It is long, it being capable of clinically large-scale use.
Specifically, realizing that the technical solution of above-mentioned purpose is as follows.
A kind of polypeptide, is made of ziconotide and penetratin.Preferably, the polypeptide passes through C by ziconotide
End connect composition with penetratin or the C-terminal of the ziconotide is connected by the N-terminal of connexon and penetratin
It connects, it is preferred that the connexon is 1 glycine.
Further, the amino acid of ziconotide is CKGKGAKCSRLMYDCCTGSCRSGKC (such as SEQ ID NO.1 institute
Show), alternatively, ziconotide is also possible to CKGKGAKCSRLMYDCCTGSCRSGKC (such as SEQ ID NO.1 institute in fused polypeptide
Show) by being less than 10, being less than 8, less than 6, what the amino acid less than 4,2 or 1 was lacked, and be mutated or be inserted into
The variant of amino acid.
Further, the penetratin can be Penetratin, tat peptide, Pep-1 peptide, S413-PV、Magainin
2 or Buforin 2.
Wherein, the trans-activator Tat of tat peptide source HIV-1, can spanning transduction membrane to intracellular.The amino acid of tat peptide
For YGRKKRRQRRR (as shown in SEQ ID NO.2), alternatively, tat peptide is also possible to YGRKKRRQRRR (such as in fused polypeptide
Shown in SEQ ID NO.2) by being less than 10, being less than 8,6 are less than, is carried out less than 4,2 or 1 amino acid scarce
It loses, the variant or its peptidomimetic of the amino acid of mutation or insertion.
Preferably, the amino acid sequence of foregoing polypeptides or fused polypeptide or modified ziconotide is CKGKGAKCSRLMY
DCCTGSCRSGKCGYGRKKRRQRRR (as shown in SEQ ID NO.5) is less than 6 alternatively, by being less than 10, being less than 8,
The variant or its peptidomimetic for the amino acid that amino acid less than 4,2 or 1 is lacked, is mutated or is inserted into.
The peptidomimetic, refer to with the substantially identical structure of peptide and/or functional character that are made of natural amino acid
Synthesize chemical compound.Peptidomimetic can fully include the non-natural analogs or part native peptides ammonia of the synthesis of amino acid
The chimeric molecule of base acid and some non-natural amino acid analogue.Peptidomimetic can also mix any amount of natural amino acid conservative
Site is replaced, as long as such replace the structure for not substantially changing analogies and/or inhibitory activity or combine activity.Polypeptide simulation
Ingredient can be generally from 3 structure groups containing any combination of non-natural structural components, the non-natural structural components: a) non-
The residue linkage groups of natural amide bond (" peptide bond ") connection；B) non-natural residues of naturally occurring amino acid residue are replaced；
Or c) induce Sketch of secondary structure, that is, induce or stablize secondary structure such as β-bend, γ corner, β-pleated sheet, alpha helical conformation etc.
Residue.
A second object of the present invention is to provide a kind of pharmaceutical composition or preparations, preferably pharmaceutical preparation, further, institute
It states pharmaceutical composition or preparation/pharmaceutical preparation includes polypeptide and/or acceptable carriers of the invention.
Described pharmaceutical composition or preparation/pharmaceutical preparation may include the unit dosage form of any dosage as shown below
(that is, the dosage for being used for single administration) provides pharmaceutical composition.It can use conventional mixing, dissolution, pelletize, prepare lozenge
Agent, levigate, emulsification, packing, embedding or freeze drying process preparation.It can be used one or more convenient for be processed as activating agent can medicine
With physiologically acceptable carrier, diluent, excipient or the auxiliary material of preparation, described pharmaceutical composition is prepared in a usual manner
Or preparation/pharmaceutical preparation.Suitable preparation depends on selected administration route.
Method of application can be parenteral, intravenous, oral, subcutaneous, intra-arterial, encephalic, in intrathecal, peritonaeum, part, nose
Interior or intramuscular administration.It is preferred that intravenous application or intraperitoneal injection.Described pharmaceutical composition or preparation/medicine for parenteral administration
Object preparation is preferably sterile and substantially isotonic.For injection, activating agent can be prepared in aqueous solution, preferably physical compatibility
Buffer such as Hank's solution, Ringer's solution or physiological saline or acetate buffer in (to reduce injection site not
It is suitable).The solution can contain preparaton, such as suspending agent, stabilizer and/or dispersing agent.
Third object of the present invention is to provide a kind of purposes of polypeptide.The purposes are as follows: be used to prepare drug, preferably
, it is used to prepare analgesic, it is preferred that the analgesic acts on calcium channel.
Further, the drug can be used for treating the relevant disease of pain, pain, for example, can lead to chronic ache
Disease includes diabetes, arthritis (for example, osteoarthritis, rheumatoid arthritis and juvenile chronic arthritis), cancer or change
Toxic effect, fibromyalgia, shingles zoster, irritable bowel syndrome, vascular problem or the drepanocytosis for the treatment of.
Disease relevant to accidental general pain includes polymyalgia rheumatica, hypochondria, depression, diabetes, pernicious
Anaemia, drepanocytosis and syphilis.Disease relevant to neuropathic pain includes neuralgia (for example, trigeminal neuralgia, not being true to type
Prosopodynia and the postherpetic neuralgia as caused by shingles zoster or bleb), the neuropathy of periphery, Charcot-Marie-
Tooth disease, Friedreich ataxia, diabetes (for example, diabetic neuropathy), dietary deficiency (especially vitamin
B-12), excessive alcohol use (alcoholic neuropathy), uremia (coming from kidney failure), cancer, AIDS, hepatitis, section's roller
More ticks heat transfers, diphtheria, guillain-Barre syndrome, that develop be not the HIV infection of AIDS, leprosy, Lyme disease, multiple
Arteritis nodosa, rheumatoid arthritis, sarcoidosis, Sjogren syndrome, syphilis, systemic loupus erythematosus, and be exposed to
Toxic compounds.
Disease relevant to inflammatory pain includes: (A) arthritis disease, such as rheumatoid arthritis；Adolescent chronic closes
Section is scorching；Systemic loupus erythematosus (SLE)；Urarthritis；Chorionitis；Osteoarthritis；Psoriatic arthritis；Rigid spine
It is scorching；Conjunctivo-urethro-synovial syndrome (adjuvant arthritis)；Adult Still disease；Arthritis from virus infection；It is passed from bacterium
The arthritis of dye, for example, gonococcal arthiritis and non-gonococcal bacterial arthritis (septic arthritis)；Three-level Lyme disease；
Tuberculous arthritis；And the arthritis from fungal infection, such as blastomycosis；(B) autoimmune disease, such as Ge-bars two
Cotard, Hashimoto thyroiditis, pernicious anaemia, Addison disease, type-1 diabetes mellitus, systemic loupus erythematosus, dermatomyositis, house lattice
Human relations syndrome, lupus erythematosus, multiple sclerosis, myasthenia gravis, conjunctivo-urethro-synovial syndrome and Graves disease.(C) connective group
Knit disease, such as arthritis vertebralis, dermatomyositis and fibromyalgia；(D) inflammation caused by damaging；(E) infect, such as tuberculosis or
Matter keratitis；And (G) arthritis, such as bursal synovitis or myotenositis.The type of headache include muscle/muscle-derived head
Bitterly, vascular headache, traction property or inflammatory headache, cluster headache, hormonal headache, rebound headache or chronic nasosinusitis
Headache.
Body pain can to it is following related: the muscle disease of excessive contraction of muscle, repeatedly movement disorders, such as polymyositis
Disease, dermatomyositis, lupus, fibromyalgia, polymyalgia rheumatica and rhabdomyolysis, myalgia, infection such as muscle abscess, rotation
Insect bite dermatitis, influenza, Lyme disease, malaria, Rocky Mountain spotted fever, bird flu, common cold, social acquired pneumonia, brain
Film inflammation, monkeypox, serious acute respiratory syndrome, toxic shock syndrome, trichinosis, typhoid fever and the infection of the upper respiratory tract.
Splanchnodynia can be related to disease such as below: irritable bowel syndrome, chronic functional abdominal pain (CFAP), functional consitipation, function
Energy property indigestion, non-cardiac pectoralgia (NCCP) and chronic abdominal pain, chronic gastroenteritis, such as gastritis, inflammatory bowel disease, such as Crow
Grace disease, ulcerative colitis, microcosmic colitis, diverticulitis and enterogastritis；Interstitial cystitis；Intestines ischaemic；Cholecystitis；Door screen
Tail is scorching；Gastroesophageal reflux；Ulcer, kidney stone, urinary tract infections, pancreatitis and hernia.
Fourth object of the present invention is to provide a kind of preparation method of polypeptide of the present invention, it is preferred that can be using chemistry
The mode of synthesis prepares polypeptide of the invention.It is further preferred that prepared using solid-phase synthesis or recombinant expression method, into
One step prepares polypeptide of the invention using the full-automatic solid-phase synthesis of F-moc.
Compared with prior art, the invention has the following beneficial effects: by the way that the C-terminal of ziconotide is connect with penetratin
A kind of modified ziconotide is obtained, intramuscular injection cannot be unable to, mainly pass through brain by blood-brain barrier by overcoming ziconotide
The deficiencies of brought surgical risk is high, infection risk is high is administered in room and canalis spinalis.Polypeptide of the invention can pass through blood brain
Barrier is suitable for vein, abdominal cavity or nasal-cavity administration mode, and easy to operate, clinical risk is small, is applied by vein, abdominal cavity or nasal cavity
With the drug action time in vivo is long, and analgesic effect is excellent, and polypeptide Small side effects of the invention, is suitable for big rule
The clinical application of mould.It is quality controllable in preparation process and preparation process in addition, polypeptide preparation of the invention is simple, it is suitable for big
Scale industrialization production.
Detailed description of the invention
Fig. 1: MVIIA and MVIIA-a, b, mono- step oxidative folding HPLC of c, d analyze map；
Fig. 2: MVIIA and MVIIA-a, b, the circular dichroism spectrogram of c, d, the final concentration of polypeptide are 35 μm of ol/L, are dissolved respectively
At phosphate buffer (10mM, pH=7.2)；
Fig. 3: MVIIA and MVIIA-a, b, the inhibiting effect of c, d to CaV2.2 channel current, the dosage effect song of MVIIA
Line is as shown in Figure 3A, and the dose-effect curve of MVIIA variant is as illustrated in figs. 3 b-3e.The data of 503nhibiting concentration and value of slope are equal
On the diagram, data are indicated with average value ± standard error, every group of 5 mouse for display.It is (blue in 10 μM of L-MVIIA as shown in F figure
Color) and when 2 μM of MVIIA (red), the full cellular calcium channel electric current trace that is excited by the voltage step size from -80mv to 10mv
Superposition；G figure is that the 503nhibiting concentration of MVIIA and its variant summarizes table；
MVIIA (Fig. 4 A) is administered in Fig. 4: MVIIA and MVIIA-c hot plate pain comparison result, telocoele, tail vein administration
Internal analgesic effect after MVIIA (Fig. 4 B) and MVIIA-c (Fig. 4 C).Analgesic effect is indicated with reacting latent time.Data with
The expression of average value ± standard error, every group of 6-8 mouse.* p < 0.01 p < 0.05, * * and * * * p < 0.001 expression and physiological saline group
It is compared (data analysis is using one-way analysis of variance and Duncan multiple range test method)；
Fig. 5: MVIIA-a, b, d hot plate pain experiments are as a result, Fig. 5 A- Fig. 5 C is tail vein to MVIIA-a, b, after d polypeptide
Internal analgesic effect.The influence (%MPE) of analgesic effect maximum possible as a percentage.Data are with average value ± standard error
It indicates, every group of 8-10 mouse.* p < 0.01 p < 0.05, * * and * * * p < 0.001 expression are compared with physiological saline group；
Fig. 6: MVIIA and MVIIA-a, b, c in acetic acid twisting experiment, the analgesic effect of d, 5 after 1% acetic acid is injected intraperitoneally
To in 20 minutes, the rolling number of mouse is recorded；As schemed shown in A, after telocoele is administered 30 minutes, 1% acetic acid is injected intraperitoneally
Effect compare；As shown in panelb, after tail vein is administered 30 minutes, the effect that 1% acetic acid is injected intraperitoneally is compared；#, with physiology
Salt water group (saline) group compares；*, compared with MVIIA group；&, compared with MVIIA-C group；*, #, &, p <
0.05；* *, ### , &&&, p < 0.001.Data indicate with average value ± standard error, every group of 9-11 mouse；
Fig. 7: MVIIA and MVIIA-a, b, c, d tremble the influence of time to mouse, and telocoele gives the polypeptide of 6 μ L
(0.9nmol/kg) and physiological saline.30 minutes and 120 minutes after administration, the accumulative of mouse is trembled the time in record 5 minutes.Number
Average value ± standard error (n=12) indicates accordingly；
Fig. 8: MVIIA mass spectrogram；
Fig. 9: MVIIA-a mass spectrogram；
Figure 10: MVIIA-b mass spectrogram；
Figure 11: MVIIA-c mass spectrogram；
Figure 12: MVIIA-d mass spectrogram；
Figure 13: MVIIA and various dose MVIIA-c nasal-cavity administration after analgesic potency；
Analgesic potency when Figure 14: MVIIA-a, b, d nasal-cavity administration in hot plate pain experiments.
Specific embodiment
In order to overcome the shortcomings of ziconotide in the prior art, the present inventor passes through Qi Kaonuo by the discovery that studies for a long period of time
The C-terminal of peptide and the N-terminal of penetratin connect, and the fusogenic peptide of the modified ziconotide of acquisition is suitable for vein or abdominal cavity
Administration.In order to further study the analgesic effect of different types of modified ziconotide, present invention design synthesized it is a variety of not
The fused polypeptide of same type and structure, including, connexon is not used, the C-terminal of ziconotide and the N-terminal of penetratin are passed through
The fused polypeptide being directly connected to；The fused polypeptide for using one or more glycine to construct as connexon.Further, to above-mentioned
Different types of fused polypeptide carries out structural characterization, cell experiment, experiment in vivo and side effect confirmatory experiment, to illustrate not
The effect of the modified ziconotide of same type
For a better understanding of the technical solution of the present invention, being described in detail below with reference to embodiment.
The preparation of the different types of ziconotide fusogenic peptide of embodiment 1
4 kinds of different types of fusogenic peptides are prepared, shielded polypeptide MVIIA-a, MVIIA-b, MVIIA- are respectively designated as
c,MVIIA-d.Ziconotide is prepared simultaneously, MVIIA is named as, as control.This experiment is closed using the full-automatic solid phase of F-moc
Cheng Fa, the specific steps are as follows:
The synthesis of polypeptide: using 433A automated synthesizer (ABI, Foster City, CA) model assembled on resin by
The polypeptide and its derivative of protection.At room temperature, peptide resin is incubated for 2.5 hours in suspension, Deprotection.Suspension system is
By 10 milliliters of TFA, 0.75 gram of phenol, 0.25 milliliter of 1,2- dithioglycol, 0.5 milliliter of thioanisole and 0.5 milliliter of water
Composition.(tablet held before the breast by officials methoxycarbonyl group (Fmoc), a kind of common alkoxy carbonyl group class amino protecting group).By filtering, go to protect from polypeptide
Protect separation resin in based mixtures.Thick polypeptide precipitates in the diethyl ether solution that 150ml is pre-chilled, and using 10% glacial acetic acid as elution
Agent carries out chromatographic purifying in -25 column of sephadex G.Then, the component containing polypeptide is caught and is lyophilized, and using efficient
The purity that liquid chromatography measures thick polypeptide is 80% or so.
Polypeptide folds: MVIIA includes six cysteine residues, maintains three of them disulfide bond pattern, under oxidative conditions
Folding can produce a variety of isomers.After the screening of oxidation-reduction system, buffer, salt, concentration and temperature, choose
The efficient folded condition of two MVIIA: (a) 0.5M NH4Ac buffer (pH 7.9), wherein including 1mM GSH, 0.1mM
GSSG, 1mM EDTA, and 0.2mg/mL MVIIA；(b) 0.5M NH4Ac buffer, wherein including 1mM cysteine, 1mM
EDTA, and 0.2mg/mL MVIIA.At 4 DEG C, linear type polypeptide MVIIA is 48-72 hours and in b condition 24-48h under the conditions of a
Under be folded.
Peptide purification and characterization: after MVIIA is oxidized, first reaction mixture is acidified at (pH < 4.5) with acetic acid
Reason then filters.The direct loading of filtrate to 21.2 × 250mm of Zorbax C18 liquid-phase chromatographic column, wherein using preparation
Efficient liquid-phase chromatographic pump (Waters 2000 series, Milford, MA).C18 column first uses the buffer solution A (water of 0.1%TFA
Solution) prerinse column, 10-40% buffer solution B (the second cyanogen solution of 0.1%TFA) then is used, is carried out with the speed of 8mL/min
40 minutes linear gradient elutions.Obtained fraction is the concentrate of the MVIIA containing 90%, then we using load onto 9.4 ×
The semipreparative reversed-phase high performance liquid chromatography of 250mm Zorbax C18 liquid-phase chromatographic column is further purified.Finally, we
Using 20% acetic acid solution as eluent in -25 chromatographic column of sephadex G, final product is shifted into second from tfa salt solution transfer
In acid salt solution.The purity of polypeptide is assessed by the reversed-phase high performance liquid chromatography of analytic type, and when assessment is at every point of 1ml
Under the flow velocity of clock, using Zorbax C18 liquid-phase chromatographic column (4.6 × 250mm), with the 8-40% buffer solution B (second of 0.1%TFA
Cyanogen solution) carry out 25 minutes linear gradient elutions.Finally, final product-polypeptide purity that we obtain is 98%.
Embodiment 2: the chemical characteristic and structural characterization of different types of ziconotide fusogenic peptide
The chemical characteristic of 1.MVIIA and its variant
At 4 DEG C, buffer is handled linear polypeptide 24-48 hours, is then analyzed using high performance liquid chromatography, finds line
The folding of property polypeptide results in the appearance on a main peak and several small peaks.Buffer solution system includes 1mM glutathione,
0.1mM oxidized form of glutathione, 1mM EDTA and 0.2mg/mL linear polypeptide, the pH of solution are 7.9.Main product passes through
Purification, and assessed by analyzing reversed-phase high performance liquid chromatography, while the purity of polypeptide is determined to be greater than 98%.With
Ultraflex III TOF/TOF mass spectrograph is determined (Bruker).The polypeptide sequence prepared is as shown in table 1, one
It is as shown in Figure 1 to walk oxidative folding HPLC analysis map.
Table 1: the polypeptide sequence prepared
2. circular dichroism spectra
Polypeptide dissolves in PBS (10mM, pH=7.2) solution, and final concentration of 35 μM.At room temperature, detection 190nm is arrived
Circular dichroism spectra in 260nm wave-length coverage uses Chirascan Plus spectropolarimeter (Applied
Photophysics Ltd., Leatherhead, Surrey, UK) instrument.The detection index of setting is as follows: step
resolution 1.0nm；Speed 20nm/min, and cell path length of 1.0mm.
As shown in Fig. 2, MVIIA presents apparent β-pleated sheet structure at 195nm-205nm.We have found that TAT variant has
Similar random loop construction, and in 200nm or so, there is the apparent wave band that weakens to occur.These results indicate that MVIIA and TAT
Between catenation sequence length expand when, the secondary structure of polypeptide does not change.When catenation sequence amplification, TAT becomes
The molar ellipticity of body is deepened therewith, shows that the amplification of catenation sequence between MVIIA and TAT contributes to form random loop construction.
The accurate molecular weight of product polypeptide is identified using the method for mass spectrum (using Voyager MALDI-TOF spectrometer), such as 2 institute of table
Show, MVIIA and MVIIA-a, b, c, the mass spectrogram of d is as shown in Fig. 8-Figure 12.The bridge mode of disulfide bond is since part is reduced
What the method for cysteine coupling and amino acid silencing was allocated.The polypeptide of synthesis and the high-efficient liquid phase color of MVIIA standard items
Spectrogram and circular dichroism spectrogram are the result is that consistent.
The molecular weight of table 2.MVIIA and its variant
| Sample | Theoretical MW | Survey m/z | The difference of theoretical value and actual measurement |
| MVIIA | 2645.54 | 2639.0198 | 6.5202 |
| MVIIA-a | 4186.0784 | 4180.0108 | 6.0676 |
| MVIIA-b | 4243.0978 | 4237.0300 | 6.0678 |
| MVIIA-c | 4299.1353 | 4292.0362 | 7.0991 |
| MVIIA-d | 4356.1568 | 4351.0842 | 5.0726 |
Embodiment 3: the electrophysiology experiment of different types of ziconotide fusogenic peptide
In order to further study different type modified ziconotide electrophysiology effect and lead to calcium ion (CaV2.2)
The inhibiting effect in road, is tested as follows:
HEK293T cell (can express SV40 large T antigen) culture is containing 10% fetal calf serum, 1% penicillin, streptomysin
DMEM high glucose medium (Gibco) in.Incubator environment is 37 DEG C, 5%CO2.Dr.Diane Lipscombe is provided greatly
The α in the channel mouse CaV2.21BSplice variant e37a, auxiliary subunit α2δ1And β3Plasmid (Addgene plasmid#26569, #
26575,#26574).Wink transfects together for subsequent three kinds of plasmids (3 μ g), 0.4 μ g enhanced green fluorescent protein gene and liposome
Into HEK293T cell.Transfection 24 hours after, cell inoculation on a glass slide, incubator (37 DEG C, 5%CO2) culture
At least 6 hours, then carry out electrophysiological recording.
This research is that the method recorded according to cell voltage clamper in the Research Literature delivered before is recorded
(F.Wang et al.,2016).In simple terms, recording electrode has the resistance of about 3M Ω, is full of by internal solution.It is internal
Solution includes 135mM CsCl, 10mM NaCl, 10mM HEPES, and 5mM EGTA, and adjusting pH value of solution with CsOH is 7.2.Carefully
Recording out cell solution includes 135mM N-Methyl-D-glucamine, 10mM BaCl2.2H2O,2mM MgCl2.6H2O and
10mM HEPES, solution final pH are 7.4.(~22 DEG C) at room temperature, using MultiClamp 700B amplifier (Molecular
Devices, Sunnyvale, CA) and Clampex 10.3/Digidata1440A data collection system and digital analog converter record
The electric current of acquisition.Membrance current samples after 2kHz filtering in 10kHz.All data use data analysis system clampfit
10.3 are analyzed (Molecular Devices), and are indicated with average value ± standard error.Block the poison of N-type Ca ionic current
Dose-effect curve GraphPad Prism (GraphPad Software, San Diego, CA) Software on Drawing of element,
Using current amplitude suppression curve as the function of drug concentration, it is fitted using Hill's equation.
The primary amino acid sequence and its electrophysiologic activity of MVIIA and its variant MVIIA-a, b, c, d are as shown in table 3.
The primary amino acid sequence and its electrophysiologic activity of table 3.MVIIA and its variant
The inhibiting effect of MVIIA and its variant to the channel calcium ion (CaV2.2)
It is well known that MVIIA is a kind of selectivity CaV2.2 channel blocker.Concentration is 2 μM of 90% or more MVIIA energy
Blocking CaV2.2 access.(F.Wang.2016, and other articles) in our current research, we are in 293T cell
Have recorded the channel CaV2.2 (α1B,α2δ1 and β3) Ca2+Peak point current (ICa).All electric currents by 100ms from -80mv to
The voltage step size of 10mv is excited.MVIIA, MVIIA-a, MVIIA-b, MVIIA-c and MVIIA-d processing of 1 μM of concentration can be with
Reduce Ca2+Peak point current, decreasing value are respectively 98.24 ± 0.708%, 89.45 ± 0.752%, 91.70 ± 1.477%,
98.81 ± 0.427% and 84.26 ± 3.127%.It was found that MVIIA-c and MVIIA have similar blocking CaV2.2 channel
Ability.L-MVIIA blocks CaVThe ability in 2.2 channels significantly reduces, and be only capable of under 10 μM of concentration reduction by 23.28 ±
3.347% Ca2+Peak point current.The concentration and inhibition Ca of MVIIAVThe 503nhibiting concentration of the response relation in 2.2 channels is
It is 0.0436 μM, 5-10 times almost big compared with TAT variant.TAT variant (MVIIA-a, MVIIA-b, MVIIA-c and
MVIIA-d 503nhibiting concentration) is respectively 0.413,0.379,0.237 and 0.345 μM, as shown in Figure 3.These results indicate that
MVIIA-a, MVIIA-b, MVIIA-c and MVIIA-d are to CaV2.2 channels have certain inhibitory effect, and MVIIA and TAT
The length of catenation sequence can influence Ca between variantVThe binding ability in 2.2 channels.
Embodiment 4: the internal analgesic effect experiment of different types of ziconotide fusogenic peptide
1. hot plate pain experiments
1.1 experimental method
In this experiment, only, telocoele administration MVIIA (0.11,0.33 is respectively adopted in nine groups of mouse, every group of mouse 6-8 altogether
Or 1.00nmol/kg), MVIIA and MVIIA-a, MVIIA-b, MVIIA-c and MVIIA-d (0.33,1.00or is administered in tail vein
3.00μmol/kg).When two kinds of approach administrations, physiological saline group is used as blank control group.It is constant that animal is placed in a temperature
For on 55 ± 0.5 DEG C of electric irons, delay time is recorded from mouse to be placed on to the surface of electric iron to licking back leg for the first time
Claw or the threshold value (Eddy and Leimbach, 1953) of time for jumping up as pain index for the first time.With 60s
Time be boundary, mouse is taken out if more than 60s, mouse tissue is avoided to be damaged.Before administration, delay time is mentioned
Preceding measurement is used as baseline value；Then, record administration MVIIA, MVIIA-c and Saline (telocoele administration or tail vein administration)
Delay time when rear 0.5,1,2,3,4,6,8,10 and 12h.Compared with postponing baseline time, delay time less than 5s or
It is regarded as insensitive and hypersensitization mouse more than the mouse of 20s, is then removed.Analgesic effect is with latency
It indicates.
1.2 analgesic potencies compare
As shown in figure 4, MVIIA (0.11,0.33 and 1.00nmol/kg) is administered after 1 hour in telocoele, the drug effect of MVIIA
Reach peak；When by 4 hours, the drug effect of MVIIA disappears (Fig. 4 A) substantially.However, when tail vein injection, multi-dose
MVIIA does not generate drug effect (Fig. 4 B).MVIIA-c be MVIIA TAT variant in have most high inhibition CaV2.2 channel current effect
Variant.As shown in Figure 4 C, MVIIA-c shows strongest drug effect when being administered 3 hours, and to continue 4 small for its most strong drug effect
When or so, duration of efficacy is 12 hours.
As shown in figure 5, the MVIIA-a of tail vein injection various dose, b, d (0.11umol/kg, 0.33umol/kg and
1.00 μm of ol/kg) after 1 hour, analgesic effect is showed, and show strongest drug effect when being administered 2-3 hours, drug effect is held
It is 4 hours or so continuous, it gradually reduces at any time, administration group and salt water group still have marked difference after administration 12 hours, and drug effect is lasting
Time is 12 hours.
2. acetic acid twisting experiment (Koster et al., 1959)
2.1 experimental method
The MVIIA-a of Three doses, b, c, d polypeptide group (0.6,1.8 and 5.4nmol/kg, it is low in figure, in, high dose),
Saline control group (saline), positive reference medicine group MVIIA (0.11, the 0.33 and 1.00nmol/kg, in figure of Three doses
It is low, in, high dose) processing animal.When writhing assay, MVIIA (side brain is administered respectively within 30 minutes before 1% acetic acid is injected intraperitoneally
Room) or MVIIA-a, b, c, d (telocoele), then measure MVIIA and MVIIA-MVIIA-a, b, c, the internal analgesic effect of d.
In order to detect the ability of MVIIA and MVIIA-a, b, c, d by blood-brain barrier, 3 hours before 1% acetic acid is injected intraperitoneally, lead to
MVIIA and MVIIA-a, b, c, d is administered in the mode for crossing tail vein administration respectively.Physiological saline group is used as blank control group (side
Ventricles of the brain administration or tail vein administration).Record acetic acid injection after in 5 minutes to 20 minutes mouse rolling number (Galeotti
et al.,2008).The number of twist motion is with the contraction of abdominal muscles and along with the stretching of hind leg and the elongation of body
What feature was recorded.Suppression percentage is calculated using following equation:
Inhibition (%)=(N0–N1)×100/N0
N0Refer to the twisting number of physiological saline group, N1For the twisting number of administration group.
2.2 analgesic potencies compare
In acetic acid twisting experiment, the MVIIA-a of Three doses, b, c, d polypeptide group (0.6,1.8 and 5.4nmol/kg, figure
It is low in 6, in, high dose), saline control group (saline), positive reference medicine group MVIIA (0.11,0.33 He of Three doses
It is low in 1.00nmol/kg, Fig. 6, in, high dose) processing animal, each group is compared in three kinds of various doses, intravenously administrable and side brain
Rolling number under the conditions of the administration of room.It was found that MVIIA-a, b, c, d polypeptide group and positive reference medicine group MVIIA reduce acetic acid
The rolling number of induction, and be in dose dependent.Under the conditions of telocoele administration, MVIIA, MVIIA-a, b, c, d makes respectively
The rolling number of mouse is reduced to (relative to salt water group): MVIIA 8.97%, 53.37%, and 76.88%；MVIIA-A,
2.94%, 13.36%, 48.35%；MVIIA-B, 10.82%, 42.79%, 77.60%；MVIIA-C, 14.75%,
39.53%, 81.77%；MVIIA-D, 12.08%, 23.95%, 56.54%.Under the conditions of intravenously administrable, positive reference drug
Effect of the MVIIA without the rolling number for reducing mouse, MVIIA-a, b, it is (opposite that c, d are respectively reduced to the rolling number of mouse
In salt water group): MVIIA-a, 10.47%, 27.82%, 30.03%；MVIIA-b, 17.08%, 45.94%, 51.79%；
MVIIA-c, 19.81%, 49.30%, 62.95%；MVIIA-d, 6.33%, 35.86%, 47.57%, as shown in Figure 6.
Conclusion: it can be seen that from above-mentioned experimental result compared to MVIIA, MVIIA-a, b, c, d polypeptide can be infused in vein
The situation penetrated plays analgesic effect and is in dose dependent, in particular, in the case where high dose, MVIIA-a, b, c,
D polypeptide can reach good analgesic effect, meet clinical use demand by intravenous injection.Further, compared to MVIIA,
MVIIA-a, b, c is administered by intravenous injection, d drug effect is up to 12 hours, there is good slow release effect in vivo.
Aforementioned analgesic experiment uses one-way analysis of variance (one-way ANOVA), the multifactor variance point of duplicate measurements
Analyse (two-way ANOVA with repeated measures), between group with the method for one Ke Er test method of Duncan or Newman into
Row analysis.All data use the confidence interval of mean+SD or standard error or 95%.When the difference of p value is less than
When 0.05, it is believed that data have statistical significance.
Embodiment 5: the side effect experiment of different types of ziconotide fusogenic peptide
In order to further study the side effect of different type modified ziconotide in vivo, tested as follows:
1. experimental method
Time of trembling is considered as a kind of typical side effect of ziconotide.Tremble the time be record a period of time in mouse
Four limbs, head and the rhythmical vibration of trunk total time.Mouse is grouped at random: MVIIA (0.9nmol/kg) group, MVIIA-a,
B, c, d (0.9nmol/kg) group and Normal group (6 μ L, telocoele administration；N=12, half male and half female).Administration 30 minutes and
After 120 minutes, the dynamic video of mouse in 5 minutes is recorded with digital camera, and count every to uncomprehending people is tested by one
Accumulative in mouse 5 minutes is trembled the time.
Toxicological experiment using one-way analysis of variance (one-way ANOVA) and one Ke Er test method of Newman method into
Row analysis.All data use the confidence interval of mean+SD or standard error or 95%.When the difference of p value is less than
When 0.05, it is believed that data have statistical significance.
2.1 side effects are compared
As shown in fig. 7, at administration 30 minutes, MVIIA, which causes more obviously to tremble, symptom and longer to tremble the time；It gives
At medicine 120 minutes, each group polypeptide compared with MVIIA, caused by tremble symptom and the longer time of trembling does not have notable difference.From
As can be seen that MVIIA is in MVIIA-a, b, c in the above results, d polypeptide no significant difference in side effect is even being administered
Incipient stage, MVIIA-a, b, the side effect of c, d will also be lower than MVIIA, it is seen then that the MVIIA-a of the application, b, c, d polypeptide poison
Side effect is smaller.
The administration of the embodiment 6:MVII-A ventricles of the brain and MVIIA-a, b, c, d collunarium analgesic experiment compare
1.1 hot plate pain experiments methods
Hot plate pain experiments method is as previously described.In this experiment of mouse, nine groups of mouse altogether, every group mouse 10, the ventricles of the brain
It inside gives MVIIA (1.00nmol/kg, 5ul/10g) and is used as positive controls (finding that MVIIA intranasal administration is invalid in experiment),
Nasal cavity is given physiological saline (saline, 2ul/10g) respectively, MVIIA-C (3.3,6.6 or 9.9nmol/kg, 2ul/10g).
Physiological saline group is as blank control group.Record intracerebroventricular administration MVIIA, 0.5 after nasal-cavity administration MVIIA-c and Saline,
1, delay time when 2,3,4,6,8,10h.Compared with postponing baseline time, delay time is less than 5s or small more than 20s
Mouse is regarded as insensitive and hypersensitization mouse, is then kicked and is removed.
Analgesic effect is the influence (%MPE) of maximum possible as a percentage, is finally counted using following equation
It calculates: %MPE=(T1-T0)×100/(T2-T0)
Wherein, T0And T1Respectively indicate the delay time of administration front and back, T2It is the boundary time tested every time.
1.2 experimental result
Analgesic potency after MVIIA and various dose MVIIA-c nasal-cavity administration is as shown in figure 13.Figure 13 illustrates MVIIA
The analgesic effect when ventricles of the brain and MVIIA-c nasal-cavity administration in hot plate pain experiments.MVIIA (1.00nmol/kg) is administered in the ventricles of the brain
Afterwards, drug effect continues 4 hours.Rapid-onset after MVIIA-C (3.3,6.6,9.9nmol/kg) nasal-cavity administration, the MVIIA- of high dose
C duration of efficacy is long, and at 8 hours, still there were significant differences with physiological saline group, and drug effect disappears after administration 10 hours.*p<
0.05, * p < 0.01 * and * * * p < 0.001 expression are compared with physiological saline group.
1.3 MVIIA-a, b, d collunarium analgesic experiment
As shown in figure 14, analgesic effect when MVIIA-a, b, d nasal-cavity administration in hot plate pain experiments.With MVIIA-C
Similar, MVIIA-a, b, rapid-onset after d (9.9nmol/kg) nasal-cavity administration, MVII-b still had with physiological saline group at 8 hours
Significant difference, drug effect disappears after administration 10 hours.* p < 0.05, * * * p < 0.001 expression are compared with physiological saline group.
Above the present invention is described in detail with a general description of the specific embodiments, but in the present invention
On the basis of, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, not
These modifications or improvements on the basis of deviation spirit of that invention, fall within the scope of the claimed invention.
Sequence table
<110>Shenzhen Graduate School of Peking University
<120>a kind of to pass through the application of the polypeptide of blood-brain barrier in medicine preparation
<141> 2018-09-11
<160> 6
<170> SIPOSequenceListing 1.0
<210> 1
<211> 25
<212> PRT
<213> Artificial Sequence
<400> 1
Cys Lys Gly Lys Gly Ala Lys Cys Ser Arg Leu Met Tyr Asp Cys
1 5 10 15
Cys Thr Gly Ser Cys Arg Ser Gly Lys Cys
20 25
<210> 2
<211> 11
<212> PRT
<213> Artificial Sequence
<400> 2
Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg
1 5 10
<210> 3
<211> 38
<212> PRT
<213> Artificial Sequence
<400> 3
Cys Lys Gly Lys Gly Ala Lys Cys Ser Arg Leu Met Tyr Asp Cys
1 5 10 15
Cys Thr Gly Ser Cys Arg Ser Gly Lys Cys Gly Gly Tyr Gly Arg
20 25 30
Lys Lys Arg Arg Gln Arg Arg Arg
35
<210> 4
<211> 36
<212> PRT
<213> Artificial Sequence
<400> 4
Cys Lys Gly Lys Gly Ala Lys Cys Ser Arg Leu Met Tyr Asp Cys
1 5 10 15
Cys Thr Gly Ser Cys Arg Ser Gly Lys Cys Tyr Gly Arg Lys Lys
20 25 30
Arg Arg Gln Arg Arg Arg
35
<210> 5
<211> 37
<212> PRT
<213> Artificial Sequence
<400> 5
Cys Lys Gly Lys Gly Ala Lys Cys Ser Arg Leu Met Tyr Asp Cys
1 5 10 15
Cys Thr Gly Ser Cys Arg Ser Gly Lys Cys Gly Tyr Gly Arg Lys
20 25 30
Lys Arg Arg Gln Arg Arg Arg
35
<210> 6
<211> 39
<212> PRT
<213> Artificial Sequence
<400> 6
Cys Lys Gly Lys Gly Ala Lys Cys Ser Arg Leu Met Tyr Asp Cys
1 5 10 15
Cys Thr Gly Ser Cys Arg Ser Gly Lys Cys Gly Gly Gly Tyr Gly
20 25 30
Arg Lys Lys Arg Arg Gln Arg Arg Arg
35
Claims (3)
1. polypeptide is preparing the purposes in analgesic, which is characterized in that the polypeptide is that the C-terminal of ziconotide is passed through connection
Son is connect with the N-terminal of penetratin, and the amino acid sequence of the polypeptide is as shown in SEQ ID NO.5.
2. purposes described in claim 1, which is characterized in that the drug further includes acceptable carrier.
3. purposes described in claim 1-2 any one, the polypeptide are made vein, abdominal cavity or nasal cavity administrated preparation, pass through
Vein, abdominal cavity or nasal-cavity administration mode are applied.
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| PCT/CN2018/124255 WO2020056988A1 (en) | 2018-09-18 | 2018-12-27 | Use of polypeptide capable of passing through blood-brain barrier in the preparation of drug |
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| CN109265556B (en) * | 2018-09-18 | 2019-12-24 | 深圳瑞健生物科技有限公司 | Polypeptide suitable for intravenous, intraperitoneal or nasal administration |
| CN109232746B (en) * | 2018-09-18 | 2020-02-28 | 深圳瑞健生物科技有限公司 | Fusion polypeptide of ziconotide and TAT peptide |
| CN109232745B (en) * | 2018-09-18 | 2020-02-28 | 深圳瑞健生物科技有限公司 | Polypeptide capable of passing blood brain barrier |
| CN113855784A (en) * | 2021-09-26 | 2021-12-31 | 深圳辰扬生物科技有限公司 | Anti-depression and anti-anxiety application of polypeptide and complex |
| CN113813364A (en) * | 2021-11-09 | 2021-12-21 | 深圳辰扬生物科技有限公司 | Anti-addiction and anti-relapse application of polypeptide, complex and polypeptide |
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| WO2007054785A1 (en) * | 2005-11-08 | 2007-05-18 | Atheris Laboratories | Mu-conotoxin peptides and use thereof as a local anesthetic |
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| JP5801197B2 (en) * | 2008-09-03 | 2015-10-28 | ノノ インコーポレイテッド | Drugs and methods for the treatment of pain |
| CN104974237B (en) * | 2015-07-18 | 2019-02-12 | 济南康和医药科技有限公司 | A kind of method of segment method synthesis in solid state ziconotide |
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| CN105709211A (en) * | 2016-04-15 | 2016-06-29 | 沈阳药科大学 | Preparation and application of analgesic active peptide SYPU-AGP3 and SYPU-AGP4 |
| CN108992661B (en) * | 2018-09-18 | 2019-09-20 | 北京大学深圳研究生院 | A kind of polypeptide is preparing the application in vein, abdominal cavity or nasal-cavity administration drug |
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Effective date of registration: 20191016 Address after: 518000 Guangdong city of Shenzhen province Qianhai Shenzhen Hong Kong cooperation zone before Bay Road No. 1 building 201 room A (located in Shenzhen Qianhai business secretary Co. Ltd.) Applicant after: Shenzhen Ruijian Biotechnology Co., Ltd. Address before: 518000 no.2199 Lishui Road, Beida Park, Xili University City, Nanshan District, Shenzhen City, Guangdong Province Applicant before: Shenzhen Graduate School of Peking University |
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