CN109097007B - Microorganism solid-free drilling fluid and preparation method thereof - Google Patents
Microorganism solid-free drilling fluid and preparation method thereof Download PDFInfo
- Publication number
- CN109097007B CN109097007B CN201811061743.4A CN201811061743A CN109097007B CN 109097007 B CN109097007 B CN 109097007B CN 201811061743 A CN201811061743 A CN 201811061743A CN 109097007 B CN109097007 B CN 109097007B
- Authority
- CN
- China
- Prior art keywords
- drilling fluid
- solid
- preparation
- free drilling
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/02—Well-drilling compositions
- C09K8/04—Aqueous well-drilling compositions
- C09K8/06—Clay-free compositions
- C09K8/08—Clay-free compositions containing natural organic compounds, e.g. polysaccharides, or derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a microbe solid-free drilling fluid and a preparation method thereof, wherein a microbe consolidation technology is combined with a geological drilling technology, the microbe is introduced into a solid-free drilling fluid system based on the most extensive geological drilling application, calcium carbonate gel is generated by the induction of the metabolic life activity of the microbe, the integrity and the physical and mechanical properties of surrounding rocks of the hole wall of a loose and broken stratum are improved, the hole wall of a drilled hole is reinforced, and the problem of instability of the hole wall of the drilled hole of the loose and broken stratum in geological drilling is solved. The invention can also select other microbial strains which can induce to generate calcium carbonate gel or other gels and other polymer drilling fluid materials, and construct the microbial solid-free drilling fluid with different formulas according to the technical idea of the scheme of the invention.
Description
Technical Field
The invention particularly relates to a microorganism solid-free drilling fluid and a preparation method thereof.
Background
The instability of the hole wall of a drill hole in a loose and broken stratum is always a technical problem faced by geological drilling, and the exploration and development of mineral products are often delayed besides causing huge economic loss. At present, the problem of instability of the pore wall of a loose and broken stratum is not solved effectively. In the past, the research on the instability problem of the hole wall of the drill hole mainly focuses on two aspects, on one hand, the instability mechanism of the hole wall of the drill hole is researched, and the cause of the instability of the hole wall is revealed; on the other hand, the drilling fluid system for stabilizing the hole wall and the action mechanism thereof are researched. In the aspect of the instability mechanism of the hole wall, the fact that the surrounding rock of the hole wall has insufficient strength to resist the stress on the surrounding rock is uniformly considered to cause damage instability, namely that the instability of the hole wall is rooted and belongs to mechanical instability. In terms of stabilizing the hole wall drilling fluid system, experts and scholars in the drilling field mainly focus on how to effectively maintain the effective stress of the hole wall surrounding rock, and develop a drilling fluid system with minimum damage degree to the effective stress of the hole wall surrounding rock so as to reduce the adverse effect of the drilling fluid on the stability of the hole wall, for example, a KCl-PHPA drilling fluid system is used for inhibiting the clay expansion pressure, a NaCl-polyalcohol drilling fluid system is used for improving the membrane efficiency of shale, and a silicate drilling fluid system is used for plugging shale pores, and the like. However, drilling practices prove that these drilling fluid systems cannot completely inhibit hydration expansion of clay minerals or block the transmission of pore pressure, can only reduce the hidden trouble of pore wall instability to a certain extent, and are not suitable for the stratum which is easy to have pore wall instability. In recent years, researchers develop a bionic wall-fixing drilling fluid system which can form a gel bionic shell with strong cohesion and adhesiveness on the surface of a well wall by simulating specific functional groups in byssus proteins secreted by marine life mussels based on a bionic technology so as to improve the strength of rocks, and breakthrough from theory to practice in drilling wall fixing is realized.
However, most of these researches are directed to shale strata which are frequently encountered in oil drilling, but not to loose and broken strata which are frequently encountered in geological drilling projects such as geological prospecting drilling and engineering geological exploration drilling and are easy to cause instability of hole walls. In geological drilling engineering, when the instability of the hole wall occurs in loose and broken stratums, due to the lack of corresponding theory and technical guidance, the drilling fluid technology for treating the shale stratum and other complex stratums in petroleum drilling engineering is usually carried out, but the drilling fluid technology has little effect and high cost and is difficult to adapt to the characteristics of the geological drilling industry.
Disclosure of Invention
Aiming at a loose and broken stratum which is easy to destabilize, and aiming at avoiding in-hole accidents caused by the destabilization of a hole wall and reducing the drilling cost, the invention needs to explore an effective drilling fluid technology for stabilizing the hole wall of a drilled hole, and aims to provide a microorganism solid-free drilling fluid and a preparation method thereof.
Therefore, the invention adopts the following technical scheme:
the microbial solid-free drilling fluid is characterized by comprising a bacillus pasteurii bacterial liquid, a solid-free drilling fluid and a nutrient solution.
A preparation method of a microorganism solid-free drilling fluid is characterized by comprising the following steps: the preparation method of the microorganism solid-free drilling fluid comprises the following steps:
firstly, preparing a bacillus pasteurii bacterial liquid:
① preparation of culture Medium
Firstly, measuring purified water by using a measuring cylinder, adding the purified water into a beaker, then sequentially weighing soybean peptone, casein peptone and sodium chloride by using an electronic scale, slowly pouring the weighed soybean peptone, casein peptone and sodium chloride into the beaker after the weighing is finished, simultaneously stirring by using a glass rod to ensure that nutrient substances are completely dissolved in the purified water, and adjusting the pH value to 7.2-7.5 after the full stirring;
② Sterilization of the Medium
Transferring the culture medium into a conical flask by using a liquid transfer gun, sealing the opening of the conical flask by using kraft paper, placing the conical flask into a high-pressure steam cooker for sterilization, taking out the conical flask after sterilizing for 15 minutes under the conditions of 0.1MPa and 110 ℃, and placing the conical flask on a clean bench for natural cooling;
③ inoculation of bacterial species
Activating the pasteurella bacillus strain, burning a cone-shaped bottle mouth containing a culture medium for a circle by outer flame of an alcohol lamp, then transferring the activated strain into the culture medium by using a liquid transfer gun, and sealing the cone-shaped bottle mouth by using gauze;
④ bacteria-forming liquid
Placing the inoculated culture medium into a shaking incubator, culturing for 24 hours at 30 ℃ and 200rpm, taking out after the culture is finished, and placing the culture medium into a low-temperature storage box for storage for later use;
step two, preparing nutrient solution:
adding urea and calcium chloride into purified water to obtain a nutrient solution, wherein the concentration of urea and the concentration of calcium chloride in the prepared nutrient solution are both 1M, and fully stirring to completely dissolve the urea and the calcium chloride to obtain the nutrient solution for later use;
step three, preparing the solid-free drilling fluid:
adding vegetable gum, polyacrylamide and sodium carboxymethylcellulose into purified water, and stirring to dissolve completely;
fourthly, preparing the microorganism solid-free drilling fluid:
the prepared bacterial liquid, the solid-free drilling fluid and the nutrient solution are mixed according to the volume ratio of 2: 2: 1, mixing and stirring uniformly to finish the preparation.
In addition to the above technical solutions, the present invention also includes the following technical features.
In the preparation process of the pasteurella bacillus liquid, the pH value for preparing the culture medium is preferably 7.3.
The invention can achieve the following beneficial effects: 1. the invention combines a microorganism consolidation technology with a geological drilling technology, is based on a solid-free drilling fluid system which is most widely applied to geological drilling, introduces microorganisms into the solid-free drilling fluid system, induces and generates calcium carbonate gel through metabolic life activities of the microorganisms, improves the integrity and the physical and mechanical properties of surrounding rocks of the hole wall of a loose and broken stratum, reinforces the hole wall of a drill hole, and solves the problem of instability of the hole wall of the drill hole of the loose and broken stratum in the geological drilling. 2. In addition, other microbial strains which can induce to generate calcium carbonate gel or other gels and other polymer drilling fluid materials can be selected, and according to the technical idea of the scheme of the invention, the microbial solid-free drilling fluid with different formulas is constructed.
Detailed Description
Example (b):
(1) preparation of bacillus pasteurii liquid
① preparation of culture Medium
1000ml of purified water was first measured in a measuring cylinder and added to the beaker. Then 5g of soybean peptone, 15g of casein peptone and 5g of sodium chloride are weighed by an electronic scale in sequence. After weighing, slowly pouring the nutrient solution into a beaker, and simultaneously quickly stirring the nutrient solution by using a glass rod to ensure that the nutrient solution is completely dissolved in the purified water. After stirring well, the pH was adjusted to 7.3.
② Sterilization of the Medium
Transferring 100ml of culture medium into a conical flask by using a pipette gun, sealing the opening of the conical flask by using kraft paper, placing the conical flask into a high-pressure steam cooker for sterilization, sterilizing for 15 minutes at the temperature of 110 ℃ under the pressure of 0.1MPa, taking out, and naturally cooling on a clean bench.
③ inoculation of bacterial species
Firstly, activating the pasteurella bacillus strain, burning a circle of the mouth of a conical flask filled with a culture medium by outer flame of an alcohol lamp, then transferring the activated strain into the culture medium by using a liquid transfer gun, sealing the conical mouth by using gauze, and then fastening by using a rubber band.
④ bacteria-forming liquid
And (3) putting the inoculated culture medium into a shaking incubator, culturing for 24 hours at the temperature of 30 ℃ and the speed of 200rpm, taking out after the culture is finished, and putting the culture medium into a low-temperature storage box for storage for later use.
(2) Preparation of nutrient solution
Adding 60.06g of urea and 111g of calcium chloride into 1000ml of purified water, namely the concentration of the urea and the concentration of the calcium chloride in the prepared nutrient solution are both 1M, and fully stirring to completely dissolve the urea and the calcium chloride to obtain the nutrient solution for later use.
(3) Preparation of solid-free drilling fluid
1000ml of purified water is taken, 2.5gSM vegetable gum, 2.5g polyacrylamide and 2.5g sodium carboxymethylcellulose are respectively added, and the mixture is fully stirred to be completely dissolved for later use.
(4) Preparation of microbe solid-free drilling fluid
The prepared bacterial liquid, the solid-free drilling fluid and the nutrient solution are mixed according to the volume ratio of 2: 2: 1, mixing and stirring uniformly.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (2)
1. A preparation method of a microorganism solid-free drilling fluid is characterized by comprising the following steps: the microorganism solid-free drilling fluid comprises a bacillus pasteurii bacterial solution, a solid-free drilling fluid and a nutrient solution; the preparation method of the microorganism solid-free drilling fluid comprises the following steps:
firstly, preparing a bacillus pasteurii bacterial liquid:
① preparation of culture Medium
Firstly, measuring purified water by using a measuring cylinder, adding the purified water into a beaker, then sequentially weighing soybean peptone, casein peptone and sodium chloride by using an electronic scale, slowly pouring the weighed soybean peptone, casein peptone and sodium chloride into the beaker after the weighing is finished, simultaneously stirring by using a glass rod to ensure that nutrient substances are completely dissolved in the purified water, and adjusting the pH value to 7.2-7.5 after the full stirring;
② Sterilization of the Medium
Transferring the culture medium into a conical flask by using a liquid transfer gun, sealing the opening of the conical flask by using kraft paper, placing the conical flask into a high-pressure steam cooker for sterilization, taking out the conical flask after sterilizing for 15 minutes under the conditions of 0.1MPa and 110 ℃, and placing the conical flask on a clean bench for natural cooling;
③ inoculation of bacterial species
Activating the pasteurella bacillus strain, burning a cone-shaped bottle mouth containing a culture medium for a circle by outer flame of an alcohol lamp, then transferring the activated strain into the culture medium by using a liquid transfer gun, and sealing the cone-shaped bottle mouth by using gauze;
④ bacteria-forming liquid
Placing the inoculated culture medium into a shaking incubator, culturing for 24 hours at 30 ℃ and 200rpm, taking out after the culture is finished, and placing the culture medium into a low-temperature storage box for storage for later use;
step two, preparing nutrient solution:
adding urea and calcium chloride into purified water to obtain a nutrient solution, wherein the concentration of urea and the concentration of calcium chloride in the prepared nutrient solution are both 1M, and fully stirring to completely dissolve the urea and the calcium chloride to obtain the nutrient solution for later use;
step three, preparing the solid-free drilling fluid:
adding vegetable gum, polyacrylamide and sodium carboxymethylcellulose into purified water, and stirring to dissolve completely;
fourthly, preparing the microorganism solid-free drilling fluid:
the prepared bacterial liquid, the solid-free drilling fluid and the nutrient solution are mixed according to the volume ratio of 2: 2: 1, mixing and stirring uniformly to finish the preparation.
2. The preparation method of the microorganism solid-free drilling fluid according to claim 1, wherein the preparation method comprises the following steps: the pH of the medium was 7.3.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811061743.4A CN109097007B (en) | 2018-09-12 | 2018-09-12 | Microorganism solid-free drilling fluid and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811061743.4A CN109097007B (en) | 2018-09-12 | 2018-09-12 | Microorganism solid-free drilling fluid and preparation method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109097007A CN109097007A (en) | 2018-12-28 |
CN109097007B true CN109097007B (en) | 2020-04-24 |
Family
ID=64865892
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811061743.4A Active CN109097007B (en) | 2018-09-12 | 2018-09-12 | Microorganism solid-free drilling fluid and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109097007B (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102925397A (en) * | 2012-11-23 | 2013-02-13 | 天津亿利科能源科技发展股份有限公司 | Mixed bacterial agent for reducing viscosity of thick oil, as well as preparation method and use thereof |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110030957A1 (en) * | 2009-08-07 | 2011-02-10 | Brent Constantz | Carbon capture and storage |
US10125303B2 (en) * | 2015-07-31 | 2018-11-13 | Baker Hughes, A Ge Company, Llc | Compositions and methods for cementing a wellbore using microbes or enzymes |
-
2018
- 2018-09-12 CN CN201811061743.4A patent/CN109097007B/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102925397A (en) * | 2012-11-23 | 2013-02-13 | 天津亿利科能源科技发展股份有限公司 | Mixed bacterial agent for reducing viscosity of thick oil, as well as preparation method and use thereof |
Also Published As
Publication number | Publication date |
---|---|
CN109097007A (en) | 2018-12-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR100218206B1 (en) | Rapidly hydrating welan gum heteropolysaccharide | |
CN101747880B (en) | Deep-water low-temperature low-density early-strength cementing slurry for well cementation | |
WO2015007011A1 (en) | Preparation method of self-repairing cement-based material | |
CN103803714B (en) | A kind of method of oil degradation bacteria Synergistic degradation oil extraction waste water | |
CN102852497B (en) | A kind of compound microorganism oil extraction method for low permeability oilfield | |
CN109320192A (en) | A kind of modified filler of microorganism and preparation method | |
CN109900880A (en) | A kind of MICP test method using immobilized microorganism technique | |
CN106747176A (en) | A kind of novel gelled material and its preparation and application method with carbon sequestration capacity | |
CN107558968B (en) | A kind of method that oil well microbial compound throughput recovers the oil | |
CN104829078B (en) | Method with mixed microorganism microbial inoculum harmless treatment drilling mud | |
Xu et al. | A correlation study on optimum conditions of microbial precipitation and prerequisites for self-healing concrete | |
Qian et al. | Living concrete with self-healing function on cracks attributed to inclusion of microorganisms: Theory, technology and engineering applications—A review | |
CN112125592A (en) | Microbial repairing agent for repairing rock cracks and preparation method thereof | |
CN107312515A (en) | A kind of multi-element biologic compound oil displacement agent system and its injection technology | |
CN103333839A (en) | Mannanase, and gene and applications thereof | |
CN109097007B (en) | Microorganism solid-free drilling fluid and preparation method thereof | |
CN111576390A (en) | Method for reducing expansibility of expansive soil | |
Hu et al. | Biomineralization Performance of Bacillus sphaericus under the Action of Bacillus mucilaginosus | |
CN105567204B (en) | A method of dolostone reservoirs Central Plains oil recovery is improved using microbial flora | |
CN112813953A (en) | Device for reinforcing foundation by microorganism ultrasonic grouting and construction method thereof | |
CN104893692B (en) | High-temperature-resistant recyclable foam drilling fluid and preparation method thereof | |
Umar et al. | Performance evaluation of lime and microbial cementation in residual soil improvement | |
CN103232966A (en) | Middle and high temperature resisting exopoly water insoluble polysaccharide protoplast fusion engineering bacterium and application thereof | |
Zhong et al. | Experimental study on microbial induced calcium carbonate precipitation to enhance reservoir recovery | |
Shi et al. | Study of Concrete Crack Repair using Bacillus megaterium |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |