CN109095622B - Method for promoting sludge to extract flocculant by using metazoan - Google Patents
Method for promoting sludge to extract flocculant by using metazoan Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
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Abstract
The invention discloses a method for promoting sludge to extract a flocculating agent by utilizing metazoan, belonging to the field of sewage treatment. The invention aims to solve the problems that the existing microbial flocculant produced by utilizing excess sludge has harsh conditions, high production cost and great extraction difficulty, and the flocculant obtained by direct extraction has low flocculation rate. The method comprises the following steps: firstly, concentration and homogenization treatment; secondly, biological treatment by utilizing metazoan; and thirdly, carrying out thermal acid hydrolysis treatment. The invention is used for promoting the sludge to extract the flocculant by utilizing metazoan.
Description
Technical Field
The invention belongs to the field of sewage treatment.
Background
The main components of the microbial flocculant are protein, polysaccharide, nucleic acid and other biological macromolecular polymers, the microbial flocculant is produced by microorganisms in a cell metabolism process, and secondary metabolites of the microbial flocculant are separated through an extraction process based on physical and chemical mechanisms, so that a microbial flocculant product capable of promoting suspended particles, thalli and colloidal particles in water to settle is obtained. The microbial flocculant has the advantages of environmental friendliness, ecological safety and the like, and is concerned in the field of water treatment research and application in recent years.
Currently, there are three major technical routes of interest for the preparation of microbial flocculants, the production of flocculants being performed by culturing on a medium, preferably a microbial flocculant-producing bacterial strain.
One of the technical routes is to produce flocculants through a finer culture medium and optimize fermentation parameters. Mainly uses carbon sources of glucose, fructose, sucrose, starch and the like, nitrogen sources of peptone, urea, nitrate and the like as substrates, or substrates with rich nutrition such as yeast extract and the like, and produces the microbial flocculant by using metabolic action of microbial flocculant producing bacteria. For example, patent CN103435250A discloses a method for improving dewatering performance of activated sludge by adding a microbial flocculant, which comprises separating, purifying, and screening to obtain microbial flocculant-producing bacteria klebsiella pneumoniae from activated sludge, extracting a flocculant sample from a fermentation supernatant under the conditions of a temperature of 25-40 ℃ and a rotation speed of 100-200 r/min, performing shaking fermentation culture for 2-3 d, and using the flocculant sample for sludge dewatering. The patent CN106430627A discloses a preparation method and application of a fungus microbial flocculant, Aspergillus niger is inoculated to the initial pH of 6.0-9.0, glucose (NH4)2SO4Urea, yeast extract, etc. at 30-35 deg.C and 150-200 r/minCarrying out shake culture for 36-72 h under the condition, and collecting fermentation liquor to obtain the product. The patent CN106630063A discloses a microbial flocculant produced by paenibacillus and application thereof, wherein the paenibacillus is inoculated into a fermentation culture medium prepared from starch, sodium dihydrogen phosphate, potassium nitrate and the like, the culture temperature is 30 ℃, and the microbial flocculant is obtained by culturing in a shaking table for 2-3 days. The difficulty of the technology lies in the screening of microbial flocculant producing strains and the comprehensive research of flocculation producing characteristics, and simultaneously, the price of substrates such as glucose, yeast extract, urea and the like is higher, so that the production cost is higher. The use of inexpensive substrates needs to be explored to reduce overall production costs.
The second technical route is to use cheap food production residues, waste water and leftover substrates to culture microbial flocculant, for example, patent CN108060179A discloses a phosphorus removal microbial flocculant and a preparation method and application thereof, wherein straw, sweet potato residues and sugarcane residue crude fiber raw materials are used as substrates, and after three-stage fermentation, flocculoproducing bacteria, clostridium cellulolyticum and myxobacteria are respectively inoculated step by step to finally obtain a fermentation product flocculant. Patent CN107089712A discloses a method for preparing an ecological bionic microbial flocculant by using marine product leftovers, which comprises the steps of preparing marine product leftovers such as fish maw, fish meat scraps and the like with artificial seawater, hydrolyzing, and culturing sludge suspension discharged by ruditapes philippinarum in hydrolyzed liquid to obtain the flocculant. Patent CN105238843B discloses a microbial flocculant, a preparation method and application thereof, wherein a fermentation culture medium prepared from sweet potato processing wastewater is inoculated with a microbial flocculant producing strain, phosphate and sweet potato processing wastewater are supplemented in the fermentation process, and the microbial flocculant is obtained through 24-42h of fermentation. Patent CN102776239A discloses a method for producing microbial flocculant by mixing yeast wastewater and beer wastewater and adding phosphate to prepare a culture medium. According to the technical route, cheap nutrient substances such as food and industrial leftovers are utilized and are used as substrates of microbial flocculant-producing strains to support the growth of the microbial flocculant-producing strains through certain treatment, however, the preparation process of a culture medium is often complicated, the nutrient components of the cheap substrates are possibly insufficient, the nutrient components are often required to be added additionally or supplemented in the fermentation process, and especially when the flocculant is produced by utilizing the components such as cellulose, the cellulose is difficult to utilize, and multiple times of fermentation are required to produce a flocculant product.
The third technical route is to use the municipal solid waste-excess sludge as an additive to prepare a microbial flocculant culture medium and the application thereof, for example, patent CN105217805B discloses a method for preparing a microbial flocculant by using excess activated sludge and crop straws, mixing sterilized sludge and acidolysis straw liquid for sterilization to prepare a culture medium, and further adding phosphate and sterilized activated sludge for flocculant fermentation in the fermentation process. The patent CN103408146A discloses a method for preparing a composite flocculant by using a biological flocculant and polyaluminium chloride, wherein the preparation part of the organic biological flocculant utilizes the respective high-temperature sterilization of the residual sludge of a sewage treatment plant and the culture wastewater, and the supernatant of the sterilized residual sludge and the culture wastewater are mixed into a culture medium according to the volume ratio of 1 (6-11) for inoculating a flocculant to generate bacterial strains for fermentation to obtain the organic biological flocculant. The current technical route for preparing the culture medium by using sludge as a substrate comprises two types of sterilization sludge and sterilization sludge supernatant, and other cheap matrixes and nutrients are added to prepare the culture medium on the basis, so that the production cost of the flocculant is reduced to a certain degree. Such processes, while achieving the utilization of sludge, still require sterilization and additional nutrients.
The urban excess sludge is urban solid waste which is difficult to treat, and the excess sludge becomes a non-negligible environmental pollution source derived from the sewage treatment industry due to high yield and large pollution. The microbial flocculant produced by utilizing excess sludge has the advantages of environmental friendliness, ecological safety, low price and the like, and is concerned in the field of water treatment research and application in recent years. The traditional technical route usually focuses on preparation of a culture medium by using sterilized sludge, other nutrient substances are required to be additionally added due to insufficient nutrient components, and meanwhile, the fermentation conditions of flocculant-producing bacteria are harsh, so that the overall production cost and the technical requirement are still high. The biomass in the excess sludge in the form of microbial aggregates-zoogloea accounts for 60-70% of the total dry weight, the excess sludge contains a large amount of microbial cells and is rich in protein and polysaccharide, the outside of the cells is wrapped by capsules and mucus layers of the protein and the polysaccharide as main components, if the flocculation substances in the cells can be directly extracted and utilized, the excess sludge is used as a direct production raw material to extract the microbial flocculant, the complicated flocculant production processes of additional culture medium sterilization, preparation, fermentation and the like are omitted, the costs of energy consumption and material consumption of nutrient substrate addition and fermentation culture are saved, the popularization and the application in underdeveloped areas are facilitated, and the resource utilization of the sludge can be realized. However, the extraction of the flocculation active substances is difficult due to the complex components of the sludge flocs.
In summary, the existing microbial flocculant produced by using excess sludge has harsh conditions, high production cost and great extraction difficulty, and the flocculation rate of the directly extracted flocculant is low, generally lower than 30%.
Disclosure of Invention
The invention provides a method for promoting sludge to extract a flocculant by using metazoan, aiming at solving the problems of harsh conditions, high production cost, high extraction difficulty and low flocculation rate of a directly extracted flocculant in the conventional method for producing the microbial flocculant by using excess sludge.
A method for promoting sludge to extract flocculant by utilizing metazoan is carried out according to the following steps:
firstly, concentration homogenization treatment:
standing the residual sludge which is not dewatered in the sewage treatment plant, precipitating for 2-3 h, removing supernatant, taking the concentrated and precipitated sludge, and filtering by using 4-mesh and 10-mesh screens in sequence to remove impurities to obtain concentrated and homogenized sludge;
secondly, biological treatment by utilizing metazoan:
diluting the sludge after concentration and homogenization to the concentration of 3000 mg/L-5000 mg/L by using distilled water to obtain sludge mixed liquor, and adding metazoan into the sludge mixed liquor to carry out biological cell lysis treatment to obtain the sludge after metazoan treatment;
if the metazoan are large metazoan, 12g to 20g of large metazoan are added into every 1L of sludge mixed liquor under the conditions that the temperature is 22 ℃ to 26 ℃ and the concentration of dissolved oxygen is 2mg/L to 4mg/L, and the treatment is carried out for 3 days to 5 days;
if the metazoan are small metazoan, 50 to 200 small metazoan are added into every 1mL of sludge mixed liquor under the conditions that the temperature is 22 to 26 ℃ and the concentration of dissolved oxygen is more than 2mg/L, and the treatment is carried out for 3 to 5 days;
if the metazoan is the combination of the large metazoan and the small metazoan, 12g to 20g of the large metazoan is added into each 1L of sludge mixed liquor under the conditions that the temperature is 22 ℃ to 26 ℃ and the concentration of dissolved oxygen is 2mg/L to 4mg/L, and 10 to 100 small metazoans are added into each 1mL of sludge mixed liquor for 3 days to 5 days;
if the metazoan is the combination of the large metazoan and the planktonic metazoan, under the conditions that the temperature is 22-26 ℃ and the dissolved oxygen concentration is 3.5-4 mg/L, 12-20 g of the large metazoan is added into each 1L of sludge mixed liquid, and 2-5 planktonic metazoans are added into each 1mL of sludge mixed liquid for 3-5 days;
if the metazoan is the combination of the small metazoan and the planktonic metazoan, 50 to 200 small metazoans are added into each 1mL of sludge mixed solution under the conditions that the temperature is 22 to 26 ℃ and the concentration of dissolved oxygen is more than 3.5mg/L, and 2 to 5 planktonic metazoans are added into each 1mL of sludge mixed solution and are treated for 3 to 5 days;
the large metazoan is one or a combination of more of limnodrilus, tubificidae and branchia lumbricus; the small-sized epigenetic animal is aeolosoma hemprichi; the planktonic metazoan is daphnia;
thirdly, thermal acid hydrolysis treatment:
concentrating and precipitating the sludge treated by the metazoan, filtering by using a filter screen, removing supernatant liquid to separate the sludge from the metazoan, centrifuging the separated sludge for 5-10 min under the condition that the rotating speed is 2500-3000 r/min, and removing the supernatant liquid to obtain sludge solid; the water content of the sludge solid is 85-90%; adding 30-36% by mass of HCl solution into 10-20 g of sludge solids to obtain acidified sludge, then adding distilled water into the acidified sludge to obtain acidolysis sludge solution, wherein the volume of the acidolysis sludge solution is 100mL, then performing acidolysis at 30-50 ℃ for 20-30 min, centrifuging at 8000-10000 r/min for 10-15 min, and taking supernatant as a flocculant, thereby completing the method for extracting the flocculant by using the metazoan to promote sludge;
the concentration of HCl in the acidolysis sludge solution is 0.5-1 mol/L.
The invention has the beneficial effects that:
the urban excess sludge is urban solid waste which is difficult to treat, and the microbial flocculant produced by utilizing the excess sludge has the advantages of environmental friendliness, ecological safety, low price and the like, so that the microbial flocculant attracts much attention in the field of water treatment research and application in recent years. The invention discloses a method for improving the efficiency of a sludge-based flocculant, wherein the biomass in the excess sludge in the form of microbial aggregates-zoogloea accounts for 60-70% of the total dry weight, contains a large amount of microbial cells and is wrapped by capsules and mucus layers which take protein and polysaccharide as main components, the method can be used for directly extracting and utilizing flocculation substances of the excess sludge, and the microbial flocculant is extracted by taking the excess sludge as a direct production raw material.
The invention is used for a method for promoting sludge to extract a flocculating agent by using metazoan.
Detailed Description
The first embodiment is as follows: the method for promoting the sludge to extract the flocculant by utilizing the metazoan in the embodiment is carried out according to the following steps:
firstly, concentration homogenization treatment:
standing the residual sludge which is not dewatered in the sewage treatment plant, precipitating for 2-3 h, removing supernatant, taking the concentrated and precipitated sludge, and filtering by using 4-mesh and 10-mesh screens in sequence to remove impurities to obtain concentrated and homogenized sludge;
secondly, biological treatment by utilizing metazoan:
diluting the sludge after concentration and homogenization to the concentration of 3000 mg/L-5000 mg/L by using distilled water to obtain sludge mixed liquor, and adding metazoan into the sludge mixed liquor to carry out biological cell lysis treatment to obtain the sludge after metazoan treatment;
if the metazoan are large metazoan, 12g to 20g of large metazoan are added into every 1L of sludge mixed liquor under the conditions that the temperature is 22 ℃ to 26 ℃ and the concentration of dissolved oxygen is 2mg/L to 4mg/L, and the treatment is carried out for 3 days to 5 days;
if the metazoan are small metazoan, 50 to 200 small metazoan are added into every 1mL of sludge mixed liquor under the conditions that the temperature is 22 to 26 ℃ and the concentration of dissolved oxygen is more than 2mg/L, and the treatment is carried out for 3 to 5 days;
if the metazoan is the combination of the large metazoan and the small metazoan, 12g to 20g of the large metazoan is added into each 1L of sludge mixed liquor under the conditions that the temperature is 22 ℃ to 26 ℃ and the concentration of dissolved oxygen is 2mg/L to 4mg/L, and 10 to 100 small metazoans are added into each 1mL of sludge mixed liquor for 3 days to 5 days;
if the metazoan is the combination of the large metazoan and the planktonic metazoan, under the conditions that the temperature is 22-26 ℃ and the dissolved oxygen concentration is 3.5-4 mg/L, 12-20 g of the large metazoan is added into each 1L of sludge mixed liquid, and 2-5 planktonic metazoans are added into each 1mL of sludge mixed liquid for 3-5 days;
if the metazoan is the combination of the small metazoan and the planktonic metazoan, 50 to 200 small metazoans are added into each 1mL of sludge mixed solution under the conditions that the temperature is 22 to 26 ℃ and the concentration of dissolved oxygen is more than 3.5mg/L, and 2 to 5 planktonic metazoans are added into each 1mL of sludge mixed solution and are treated for 3 to 5 days;
the large metazoan is one or a combination of more of limnodrilus, tubificidae and branchia lumbricus; the small-sized epigenetic animal is aeolosoma hemprichi; the planktonic metazoan is daphnia;
thirdly, thermal acid hydrolysis treatment:
concentrating and precipitating the sludge treated by the metazoan, filtering by using a filter screen, removing supernatant liquid to separate the sludge from the metazoan, centrifuging the separated sludge for 5-10 min under the condition that the rotating speed is 2500-3000 r/min, and removing the supernatant liquid to obtain sludge solid; the water content of the sludge solid is 85-90%; adding 30-36% by mass of HCl solution into 10-20 g of sludge solids to obtain acidified sludge, then adding distilled water into the acidified sludge to obtain acidolysis sludge solution, wherein the volume of the acidolysis sludge solution is 100mL, then performing acidolysis at 30-50 ℃ for 20-30 min, centrifuging at 8000-10000 r/min for 10-15 min, and taking supernatant as a flocculant, thereby completing the method for extracting the flocculant by using the metazoan to promote sludge;
the concentration of HCl in the acidolysis sludge solution is 0.5-1 mol/L.
In the first step of the specific embodiment, impurities in common water treatment systems in sewage treatment plants such as large gravel, dead leaves and plant seeds are filtered and removed, so that sludge is homogenized.
In the second step of the embodiment, partial deflocculation of the sludge flocs is realized through the tearing, phagocytosis, filter feeding and other actions of the metazoan on the sludge flocs, the sludge content is dissolved out, and sludge extracellular polymeric substances are stripped and dissolved into the sludge supernatant, so that the biological lysis of the sludge cells is realized.
The dosage of the large-sized metazoan in the second step of the embodiment is wet weight.
In the second step of the present embodiment, the large-sized metazoan can be screened for the polypide in different growth stages to improve the treatment effect according to specific environmental conditions. The separated metazoan can be recycled.
The beneficial effects of the embodiment are as follows: the urban excess sludge is urban solid waste which is difficult to treat, and the microbial flocculant produced by utilizing the excess sludge has the advantages of environmental friendliness, ecological safety, low price and the like, so that the microbial flocculant attracts much attention in the field of water treatment research and application in recent years. The biomass in the excess sludge in the form of microbial aggregates-zoogloea accounts for 60-70% of the total dry weight, contains a large amount of microbial cells, and is wrapped by capsules and mucus layers which take proteins and polysaccharides as main components.
The second embodiment is as follows: the first difference between the present embodiment and the specific embodiment is: and step two, diluting the concentrated and homogenized sludge to the concentration of 4500 mg/L-5000 mg/L by using distilled water to obtain sludge mixed liquor, and adding metazoan into the sludge mixed liquor to perform biological cell lysis treatment to obtain the sludge treated by the metazoan. The rest is the same as the first embodiment.
The third concrete implementation mode: this embodiment is different from the first or second embodiment in that: and step two, diluting the concentrated and homogenized sludge to a concentration of 3500 mg/L-5000 mg/L by using distilled water to obtain a sludge mixed solution, and adding metazoan into the sludge mixed solution to perform biological cell lysis treatment to obtain the sludge treated by the metazoan. The other is the same as in the first or second embodiment.
The fourth concrete implementation mode: the difference between this embodiment mode and one of the first to third embodiment modes is: and when the metazoan in the step two is a large metazoan, the metazoan is an immature-stage worm body in a growth and development stage, and the body length is 1 cm-1.5 cm. The others are the same as the first to third embodiments.
The fifth concrete implementation mode: the difference between this embodiment and one of the first to fourth embodiments is: and when the metazoan in the step two is a large metazoan, the metazoan is a worm body in the mature period, and the length of the worm body is 2.5 cm-4.5 cm. The rest is the same as the first to fourth embodiments.
The sixth specific implementation mode: the difference between this embodiment and one of the first to fifth embodiments is: and when the metazoan in the step two is large metazoan, 17g to 20g of the large metazoan is added into each 1L of the sludge mixed liquor under the conditions that the temperature is 22 ℃ to 26 ℃ and the concentration of dissolved oxygen is 2.5mg/L to 4mg/L, and the treatment is carried out for 3 days to 4 days. The rest is the same as the first to fifth embodiments.
The seventh embodiment: the difference between this embodiment and one of the first to sixth embodiments is: when the metazoan in the step two is the combination of the large metazoan and the planktonic metazoan, 12g to 16g of the large metazoan is added into each 1L of the sludge mixed solution under the conditions that the temperature is 22 ℃ to 24 ℃ and the concentration of dissolved oxygen is 3.5mg/L to 4mg/L, and 2 to 5 planktonic metazoans are added into each 1mL of the sludge mixed solution for 3 days to 5 days. The others are the same as the first to sixth embodiments.
The specific implementation mode is eight: the present embodiment differs from one of the first to seventh embodiments in that: and in the third step, acidolysis is carried out for 25-30 min at the temperature of 45-50 ℃. The rest is the same as the first to seventh embodiments.
The specific implementation method nine: the present embodiment differs from the first to eighth embodiments in that: and in the third step, acidolysis is carried out for 20-30 min at the temperature of 40-50 ℃. The other points are the same as those in the first to eighth embodiments.
The detailed implementation mode is ten: the present embodiment differs from one of the first to ninth embodiments in that: the concentration of HCl in the acidolysis sludge solution in the third step is 0.5-0.8 mol/L. The other points are the same as those in the first to ninth embodiments.
The following examples were used to demonstrate the beneficial effects of the present invention:
the first embodiment is as follows:
a method for promoting sludge to extract flocculant by utilizing metazoan is carried out according to the following steps:
firstly, concentration homogenization treatment:
standing the residual sludge which is not dewatered in the sewage treatment plant, precipitating for 3 hours, removing supernatant, taking the sludge after the concentrated precipitation, and filtering by using 4-mesh and 10-mesh screens in sequence to remove impurities to obtain the sludge after the concentrated homogenization;
secondly, biological treatment by utilizing metazoan:
diluting the sludge after concentration and homogenization to the concentration of 4500mg/L by using distilled water to obtain sludge mixed liquor, adding metazoan into the sludge mixed liquor to perform biological cell lysis treatment, and obtaining sludge after metazoan treatment;
the metazoan is a combination of limnodrilus, tubificidae and branchia lumbricus, and 17g of metazoan is added into each 1L of sludge mixed liquor under the conditions that the temperature is 22 ℃ and the concentration of dissolved oxygen is 2.5mg/L, and the metazoan is treated for 4 days;
the metazoan are immature insects in a growth and development stage, and the body length of the metazoan is 1 cm-1.5 cm;
thirdly, thermal acid hydrolysis treatment:
concentrating and precipitating the sludge treated by the metazoan, filtering by using a filter screen, removing supernatant liquid to separate the sludge from the metazoan, centrifuging the separated sludge for 5min under the condition that the rotating speed is 2500r/min, and removing the supernatant liquid to obtain sludge solid; the water content of the sludge solid is 85-90%; adding 36 mass percent of HCl solution into 15g of sludge solid to obtain sludge after acid addition, then adding distilled water into the sludge after acid addition to obtain acidolysis sludge solution, wherein the volume of the acidolysis sludge solution is 100mL, then carrying out acidolysis for 30min at the temperature of 40 ℃, centrifuging for 10min at the rotating speed of 10000r/min after acidolysis, and taking supernatant as a flocculant, namely completing the method for promoting sludge to extract the flocculant by using metazoans;
the concentration of HCl in the acidolysis sludge solution is 1 mol/L.
According to the general method for flocculant determination-the kaolin method, the flocculation rate was found to be 75.3%.
Example two:
a method for promoting sludge to extract flocculant by utilizing metazoan is carried out according to the following steps:
firstly, concentration homogenization treatment:
standing the residual sludge which is not dewatered in the sewage treatment plant, precipitating for 2 hours, removing supernatant, taking the sludge after the concentrated precipitation, and filtering by using 4-mesh and 10-mesh screens in sequence to remove impurities to obtain the sludge after the concentrated homogenization;
secondly, biological treatment by utilizing metazoan:
diluting the concentrated and homogenized sludge to 3500mg/L by using distilled water to obtain sludge mixed liquor, and adding metazoan into the sludge mixed liquor to perform biological cell lysis treatment to obtain sludge treated by the metazoan;
the metazoan is a combination of vermicellis and daphnia, 16g vermicellis are added into every 1L of sludge mixed liquor under the conditions that the temperature is 24 ℃ and the concentration of dissolved oxygen is 3.5mg/L, and 5 daphnia are added into every 1mL of sludge mixed liquor for 5 days;
the length of the worm body of the tubificidae in the mature period is 2.5 cm-4.5 cm;
thirdly, thermal acid hydrolysis treatment:
concentrating and precipitating the sludge treated by the metazoan, filtering by using a filter screen, removing supernatant liquid to separate the sludge from the metazoan, centrifuging the separated sludge for 5min under the condition that the rotating speed is 2500r/min, and removing the supernatant liquid to obtain sludge solid; the water content of the sludge solid is 85-90%; adding 36 mass percent HCl solution into 20g of sludge solid to obtain sludge after acid addition, then adding distilled water into the sludge after acid addition to obtain acidolysis sludge solution, wherein the volume of the acidolysis sludge solution is 100mL, then carrying out acidolysis for 25min at the temperature of 45 ℃, centrifuging for 10min at the rotation speed of 10000r/min after acidolysis, and taking supernatant as a flocculant, namely completing the method for extracting the flocculant by utilizing the sludge promoted by the metazoan;
the concentration of HCl in the acidolysis sludge solution is 0.8 mol/L.
According to the general method for flocculant determination-the kaolin method, the flocculation rate was found to be 68.2%.
Comparative experiment:
compared with the efficiency of the flocculant extracted from the sludge subjected to the biological lysis and thermal acidolysis treatment in the first embodiment, the efficiency of the flocculant extracted from the sludge subjected to the biological lysis and thermal acidolysis treatment in the first embodiment is 16.4% lower than that of the flocculant extracted in the first embodiment, so that the combination of the biological lysis and thermal acidolysis of the metazoa can obtain a better flocculation rate, and the efficiency of the sludge-based flocculant is remarkably improved.
The residual sludge which is not dehydrated in the sewage treatment plant in the first embodiment, the second embodiment and the comparative experiment is the residual sludge which is not dehydrated in a certain sewage treatment plant in Harbin city.
Claims (1)
1. A method for promoting sludge to extract flocculant by using metazoan is characterized in that the method for promoting sludge to extract flocculant by using metazoan is carried out according to the following steps:
firstly, concentration homogenization treatment:
standing the residual sludge which is not dewatered in the sewage treatment plant, precipitating for 3 hours, removing supernatant, taking the sludge after the concentrated precipitation, and filtering by using 4-mesh and 10-mesh screens in sequence to remove impurities to obtain the sludge after the concentrated homogenization;
secondly, biological treatment by utilizing metazoan:
diluting the concentrated and homogenized sludge to the concentration of 4500mg/L by using distilled water to obtain sludge mixed liquor, and adding metazoan into the sludge mixed liquor to perform biological lysis treatment to obtain sludge treated by the metazoan;
the metazoan is a combination of limnodrilus, tubificidae and branchia lumbricus, and 17g of metazoan is added into each 1L of sludge mixed liquor under the conditions that the temperature is 22 ℃ and the concentration of dissolved oxygen is 2.5mg/L, and the metazoan is treated for 4 days;
the metazoan are immature insects in a growth and development stage, and the body length of the metazoan is 1 cm-1.5 cm;
thirdly, thermal acid hydrolysis treatment:
concentrating and precipitating the sludge treated by the metazoan, filtering by using a filter screen, removing supernatant liquid to separate the sludge from the metazoan, centrifuging the separated sludge for 5min under the condition that the rotating speed is 2500r/min, and removing the supernatant liquid to obtain sludge solid; the water content of the sludge solid is 85-90%; adding 36 mass percent of HCl solution into 15g of sludge solid to obtain sludge after acid addition, then adding distilled water into the sludge after acid addition to obtain acidolysis sludge solution, wherein the volume of the acidolysis sludge solution is 100mL, then carrying out acidolysis for 30min at the temperature of 40 ℃, centrifuging for 10min at the rotating speed of 10000r/min after acidolysis, and taking supernatant as a flocculant, namely completing the method for promoting sludge to extract the flocculant by using metazoans;
the concentration of HCl in the acidolysis sludge solution is 1 mol/L;
according to the general method for flocculant determination-the kaolin method, the flocculation rate was found to be 75.3%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| SU1733477A1 (en) * | 1990-05-29 | 1992-05-15 | Кировский биохимический завод | Method for preparation of flocculant from activated sludge |
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| CN103253840B (en) * | 2013-05-16 | 2014-12-10 | 广东工业大学 | Method for extracting natural acid-pickling corrosion inhibitor from surplus sludge and application thereof |
| CN106830589A (en) * | 2017-02-04 | 2017-06-13 | 协赛(上海)生物科技有限公司 | A kind of method that sludge sour water solution extracts microbial amino acid |
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