CN109061214B - Porous sample injection device - Google Patents
Porous sample injection device Download PDFInfo
- Publication number
- CN109061214B CN109061214B CN201811291134.8A CN201811291134A CN109061214B CN 109061214 B CN109061214 B CN 109061214B CN 201811291134 A CN201811291134 A CN 201811291134A CN 109061214 B CN109061214 B CN 109061214B
- Authority
- CN
- China
- Prior art keywords
- sample
- sample adding
- stepping motor
- hole
- vacuum chuck
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002347 injection Methods 0.000 title claims abstract description 62
- 239000007924 injection Substances 0.000 title claims abstract description 62
- 238000005070 sampling Methods 0.000 claims abstract description 25
- 230000007246 mechanism Effects 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims description 43
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 claims description 29
- 238000007789 sealing Methods 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims description 2
- 238000001514 detection method Methods 0.000 description 20
- 238000000034 method Methods 0.000 description 16
- 230000008569 process Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 4
- 238000009434 installation Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 238000010191 image analysis Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000005693 optoelectronics Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
- G01N35/1067—Multiple transfer devices for transfer to or from containers having different spacing
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides a porous sample injection device, and relates to the technical field of cell counting devices. The porous sampling device comprises: the device comprises a mounting frame, a motor unit, a porous sample adding disc, a vacuum chuck mechanism and a control system. The motor group comprises a first linear stepping motor, a second linear stepping motor and a rotary stepping motor. The porous sample adding disc is connected with the rotary stepping motor, the first linear stepping motor is arranged on the mounting frame, the rotary stepping motor is connected with the first linear stepping motor, and a plurality of sample adding holes are distributed on the porous sample adding disc. The vacuum chuck mechanism comprises a chuck support and a vacuum chuck, the chuck support is connected with a second linear stepping motor, the vacuum chuck is arranged on the chuck support, and the position of the vacuum chuck, the position of the sample feeding hole and the position of the sample counting chip sample feeding hole correspond. The control system can control the operation or stop of the motor unit. The porous sampling device can facilitate an operator to add a plurality of samples to be tested at a time, and save the sample adding operation time required by detecting the cell samples.
Description
Technical Field
The invention relates to the technical field of cell counting devices, in particular to a porous sample injection device.
Background
In a large number of cell biology research experiments, concentration detection (quantitative counting) of cells or other biological particles is required, and cell suspension concentration (or number) is a monitoring parameter of cell culture and a necessary parameter in many experimental projects, and is a very basic but important process condition for successfully completing the experiment. The existing counting means mainly comprise a manual counting method adopting a cell counting plate, an automatic counting instrument based on an image analysis technology and an automatic counting instrument utilizing a resistance method (Coulter principle).
Among them, the manual counting method is most common, and an experimenter injects a suspended cell sample into a cell counting plate counting cell, and performs manual counting under a microscope by visual observation and according to rules. The main disadvantages of this method are:
1. this results in a layered suspension of the cell sample after injection, since the depth of the counting cell itself is several times the cell size. Thus, the observed cell morphology is different, which results in inaccurate counting results and erroneous judgment of cell activity.
2. The samples injected into the cell counting plate on a regular basis were 10uL, but the amount of sample in the microscopic observation area was only a small fraction, less than 1uL. So that whether the cell sample is uniformly distributed in the counting cell has a great influence on the result.
3. When counting, the number is counted manually according to a certain rule, and the difference of the level of operators and the fatigue caused by visual observation introduce great human errors.
While automated instruments based on image analysis techniques avoid difficulties with visual inspection, the following deficiencies still remain:
1. the disposable counter consumable is introduced, and the user detection cost is increased.
2. The counting plate is similar to a cell counting plate in structure, so that the problems of inaccurate results and misjudgment of activity caused by layering and suspending cells on the counting plate also exist.
3. Like manual counting, most instruments based on image methods have the problem of large result deviation caused by small detection sample amount.
Although the automatic counting instrument (cytometer) using the resistance method (coulter principle) can avoid the disadvantages and shortcomings of the above 2 counting methods, the following disadvantages still exist in operation and use:
when a plurality of cell samples need to be detected, an operator needs to add samples and then wait for the result to add the next sample for detection, and a plurality of samples to be detected cannot be added at one time.
Disclosure of Invention
The invention aims to provide a porous sample injection device which can facilitate an operator to add a plurality of samples to be tested at one time and save sample addition operation time required by detecting a cell sample.
Embodiments of the present invention are implemented as follows:
the embodiment of the invention provides a porous sample injection device, which is used for sample injection of a cell counter, the cell counter comprises a shell, a counting chip and a trypan blue liquid outlet mechanism, the counting chip and the trypan blue liquid outlet mechanism are arranged in the shell, and the porous sample injection device comprises:
the mounting frame is used for being erected in the shell of the cell counter;
the motor group comprises a first linear stepping motor, a second linear stepping motor and a rotary stepping motor;
the disk part of the porous sample adding disk is connected with the output end of the rotary stepping motor, the first linear stepping motor is arranged on the mounting frame, the rotary stepping motor is connected with the output end of the first linear stepping motor and can move up and down under the drive of the first linear stepping motor, and a plurality of sample adding holes which are axially formed in the position, close to the edge, of the porous sample adding disc are distributed;
the vacuum chuck mechanism comprises a chuck support and a vacuum chuck, the chuck support is connected with the output end of the second linear stepping motor, the vacuum chuck is arranged on the chuck support and is positioned above the porous sample adding disc, the position of the vacuum chuck at least corresponds to the position of one sample adding hole, the position of at least one vacuum chuck corresponds to the position of a sample adding hole of the counting chip, and the porous sample adding disc is positioned between the vacuum chuck and the counting chip;
the control system can control the motor group to work or stop;
when in sample adding, a sample is added to one sample adding hole at the sample adding position, the control system controls the rotary stepping motor to work, and the porous sample adding disc rotates to enable the next sample adding hole to be at the sample adding position;
during sample injection, the porous sample adding disc rotates under the drive of the rotary stepping motor, so that the sample adding hole after sample addition moves to the lower part of the vacuum chuck and stops, the control system controls the first linear stepping motor to work so that the rotary stepping motor descends and the porous sample adding disc descends to be attached to the counting chip, the sample adding hole is communicated with the sample adding hole, the control system controls the second linear stepping motor to work so that the vacuum chuck descends and is attached to the porous sample adding disc, the sample adding hole, the vacuum chuck and the counting chip sample adding hole form a sealing pipeline, and a sample in the sample adding hole can enter the counting chip sample adding hole under the action of a pressure source connected with the counting chip.
In addition, the porous sample injection device provided by the embodiment of the invention can also have the following additional technical characteristics:
in an alternative embodiment of the present invention, the motor group further includes a third linear stepper motor, the third linear stepper motor is mounted on the mounting frame, and an output end of the third linear stepper motor is in transmission connection with a liquid outlet component of the trypan blue liquid outlet mechanism and can enable the liquid outlet component to move up and down;
when trypan blue mixing is needed, the control system controls the third linear stepping motor to work, so that the liquid outlet component rises, the first linear stepping motor works and enables the porous sample adding disc to descend, the second linear stepping motor works and enables the vacuum chuck to descend, and the liquid outlet component, the sample adding hole and the vacuum chuck form a sealing pipeline.
In an alternative embodiment of the present invention, the number of the vacuum chucks is two, one vacuum chuck is located corresponding to the liquid outlet component of the trypan blue liquid outlet mechanism, and the other vacuum chuck is located corresponding to the counting chip sample inlet hole.
In an alternative embodiment of the present invention, the multi-well loading plate has 25 of the loading wells, wherein one of the loading wells is a primary calibration well.
In an alternative embodiment of the present invention, the sample adding hole has a sample adding section and a sample adding section, the sample adding section is communicated with the sample adding section, the sample adding section and the sample adding section are arranged in a non-straight way, the sample adding section is used for accommodating a sample, and the sample adding section is used for allowing the sample to flow out to a counting chip or for allowing trypan blue of a trypan blue liquid outlet mechanism to enter the sample adding hole.
In an alternative embodiment of the present invention, the inner wall of the sample application section is divided into a straight barrel portion and a conical barrel portion, the straight barrel portion and the conical barrel portion are connected, the diameter dimension of the cross-sectional profile of the conical barrel portion gradually decreases from one end near the straight barrel portion to the other end, and the sample application section is connected with the side wall of the conical barrel portion.
In an alternative embodiment of the invention, sealing gaskets are arranged at the upper end and the lower end of the sample adding hole.
In an alternative embodiment of the present invention, the porous sample injection device further includes a sample injection hole cover, the motor group further includes a dual-motion motor, the dual-motion motor is mounted on the mounting frame, an output end of the dual-motion motor is connected with the sample injection hole cover, the sample injection hole cover is located above a sample injection position, and the control system can control the dual-motion motor to work, so that the sample injection hole cover and the housing block the sample injection hole at the sample injection position or move the sample injection hole cover and expose the sample injection hole at the sample injection position.
In an alternative embodiment of the present invention, the multi-hole sample injection device further includes a magnetic position sensor and a photoelectric encoder, the magnetic position sensor is installed on the installation frame and is used for positioning a rotation initial position of the multi-hole sample injection disk, the photoelectric encoder is installed on the rotation stepping motor and feeds back a rotation angle signal of the multi-hole sample injection disk to the control system, and the control system controls the rotation stepping motor to work or stop according to the rotation initial position and the rotation angle signal of the multi-hole sample injection disk, so that the sample injection hole can move to a working position.
In an alternative embodiment of the present invention, the multi-hole sample injection device further includes a photoelectric sensor, where the photoelectric sensor can detect a position of the multi-hole sample injection tray in an up-down direction and can feed back the position of the multi-hole sample injection tray in the up-down direction to the control system, and the control system controls the first linear stepper motor to work according to the position of the multi-hole sample injection tray in the up-down direction, so that the multi-hole sample injection tray rises or falls.
The beneficial effects of the invention are as follows:
the porous sampling device is matched with the motor unit and the position detection assembly through the porous sampling disc, an operator can carry out sampling of a plurality of samples during sampling, then follow-up detection is carried out on the samples in each sampling hole respectively, and the detection is carried out by one sample and one sample as in the conventional cell counter. Therefore, sample adding operation time required by detecting the cell sample is saved, the workload of operators is reduced, and the efficiency of the whole detection process is further improved. And the instrument is not required to be kept all the time, so that an operator can conveniently process other transactions in the detection process.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a porous sample injection device according to an embodiment of the present invention after being disposed inside a cytometer with a portion of a housing cut away;
FIG. 2 is a schematic view of a porous sample injection device in the cytometer with portions of the housing removed from FIG. 1;
FIG. 3 is an enlarged partial view of portion A of FIG. 2;
FIG. 4 is a schematic view of another view of FIG. 2;
FIG. 5 is a schematic illustration of a multi-well loading plate;
FIG. 6 is a cross-sectional view of the loading well.
Icon: 100-a porous sample injection device; 10-mounting frame; 31-a first linear stepper motor; 33-a second linear stepper motor; 35-a third linear stepper motor; 37-rotating a stepper motor; 39-double motion motor; 50-a porous loading plate; 51-a sample addition hole; 510, a sample adding section; 512-sample introduction section; 5121-a straight barrel portion; 5123-a cone section; 53-a sealing gasket; 55-a sample adding hole cover; 57-magnetic position sensor; 59-a photosensor; 61-an optoelectronic encoder; 71-a suction cup holder; 73-vacuum chuck; 200-a housing; 301-sample injection holes; 401-liquid outlet component.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present invention, it should be noted that, the azimuth or positional relationship indicated by the terms "upper", "lower", etc. are based on the azimuth or positional relationship shown in the drawings, or the azimuth or positional relationship in which the inventive product is conventionally put in use, are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific azimuth, be configured and operated in a specific azimuth, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed", "connected" and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In the present invention, unless expressly stated or limited otherwise, a first feature may include first and second features directly contacting each other, either above or below a second feature, or through additional features contacting each other, rather than directly contacting each other. Moreover, the first feature being above, over, and on the second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being below, beneath, and beneath the second feature includes the first feature being directly below and obliquely below the second feature, or simply indicates that the first feature is less level than the second feature.
Examples
Referring to fig. 1 to 6, the present embodiment provides a multi-hole sample injection device 100 for sample injection of a cytometer. The cell counter comprises a shell 200, a counting chip and a trypan blue liquid outlet mechanism, wherein the counting chip and the trypan blue liquid outlet mechanism are arranged in the shell 200. The multi-well sampling apparatus 100 includes:
the mounting frame 10, the mounting frame 10 is used for being erected in the shell 200 of the cell counter;
a motor group including a first linear stepping motor 31, a second linear stepping motor 33, and a rotary stepping motor 37;
the porous sample loading tray 50, the tray part of the porous sample loading tray 50 is connected with the output end of the rotary stepping motor 37, the first linear stepping motor 31 is installed on the installation frame 10, the rotary stepping motor 37 is connected with the output end of the first linear stepping motor 31 and can move up and down under the drive of the first linear stepping motor 31, and a plurality of sample loading holes 51 which are axially formed are distributed at the position, close to the edge, of the porous sample loading tray 50;
the vacuum chuck mechanism comprises a chuck support 71 and a vacuum chuck 73, the chuck support 71 is connected with the output end of the second linear stepping motor 33, the vacuum chuck 73 is arranged on the chuck support 71 and is positioned above the porous sample adding disc 50, the position of the vacuum chuck 73 at least corresponds to the position of one sample adding hole 51, the position of at least one vacuum chuck 73 corresponds to the position of the counting chip sample adding hole 301, and the porous sample adding disc 50 is positioned between the vacuum chuck 73 and the counting chip;
the control system can control the working or stopping of the motor unit;
when in sample adding, a sample is added to one sample adding hole 51 at the sample adding position, the control system controls the rotary stepping motor 37 to work, the porous sample adding disc 50 rotates and the next sample adding hole 51 is positioned at the sample adding position;
during sample injection, the porous sample adding disc 50 rotates under the driving of the rotary stepping motor 37, so that the sample adding hole 51 after sample injection moves to the lower part of the vacuum chuck 73 and stops, the control system controls the first linear stepping motor 31 to work, so that the rotary stepping motor 37 descends and the porous sample adding disc 50 descends to be attached to the counting chip, the sample adding hole 51 is communicated with the sample adding hole 301, the control system controls the second linear stepping motor 33 to work, so that the vacuum chuck 73 descends and is attached to the porous sample adding disc 50, the sample adding hole 51, the vacuum chuck 73 and the counting chip sample adding hole 301 form a sealing pipeline, and a sample in the sample adding hole 51 can enter the counting chip sample adding hole 301 under the action of a pressure source connected with the counting chip.
The architecture of the control system and the hardware used can refer to the control system of the existing cytometer. The counting chip, the trypan blue liquid outlet mechanism, the pressure source (such as a positive pressure air pump or a liquid pump) and the like can refer to the existing cell counter, and the embodiment does not improve the devices, and is not repeated here.
The sample loading position is an opening of the housing 200 for loading a sample, and when the sample loading hole 51 is rotated, a sample can be externally loaded into the sample loading hole 51.
If the activity detection is not needed, the cell counter does not include the trypan blue liquid outlet mechanism, and other structures are unchanged and are not affected.
Specifically, the motor group further comprises a third linear stepping motor 35, the third linear stepping motor 35 is installed on the installation frame 10, and the output end of the third linear stepping motor 35 is in transmission connection with a liquid outlet component 401 of the trypan blue liquid outlet mechanism and can enable the liquid outlet component 401 to move up and down;
when trypan blue mixing is required, the control system controls the third linear stepping motor 35 to work, so that the liquid outlet component 401 ascends, the first linear stepping motor 31 works and the porous sample loading disc 50 descends, the second linear stepping motor 33 works and the vacuum chuck 73 descends, and the liquid outlet component 401, the sample loading hole 51 and the vacuum chuck 73 form a sealed pipeline. The liquid outlet member 401 has a trypan blue liquid outlet.
In this embodiment, the number of vacuum chucks 73 is two, one vacuum chuck 73 is located corresponding to the liquid outlet member 401 of the trypan blue liquid outlet mechanism, and the other vacuum chuck 73 is located corresponding to the counting chip sample inlet 301.
If trypan blue mixing is to be performed, one sample adding hole 51 moves to the liquid outlet part 401 and corresponds to the trypan blue liquid outlet position after sample adding, then a sealing pipeline is formed through the operation of a motor unit, sample mixing is performed by means of a pressure source, then the porous sample adding disc 50 is rotated through the operation of the rotary stepping motor 37, so that the sample adding hole 51 after sample mixing moves to a position corresponding to the sample adding hole 301 of the counting chip, and subsequent sample adding operation is performed.
If the sample mixing is not needed, the porous sample adding disc 50 is rotated by directly rotating the stepping motor 37, so that the sample adding holes 51 are operated to the positions corresponding to the sample adding holes 301 of the counting chip, and the subsequent sample adding operation is performed.
In this embodiment, the multi-well loading plate 50 has 25 loading wells 51, with one loading well 51 being the initial calibration well. The initial calibration hole is a hole used by the cell counter during initialization, and before sample addition detection is carried out, the cell counter firstly runs a motor unit according to one sample addition hole 51, so that the position of the sample addition hole 51 can be ensured to correspond to the sample addition position, a counting chip, a trypan blue liquid outlet and the like which need to be used for devices or positions of the sample addition hole 51 according to the working state of the cell counter. Any one of the sample adding holes 51 can be used as an initial adjusting hole and used as a number 0 hole when the cell counter starts to work, and the other 24 sample adding holes 51 are used as sample holes when the cell counter actually works and used for placing samples so as to carry out subsequent work.
Besides the scheme of 25 sample adding holes 51, other schemes can be conceivable, so that operators meeting the use requirements can select instruments with different specifications, and the efficiency of sample detection is improved.
In detail, the sample loading hole 51 has a sample loading section 510 and a sample loading section 512, the sample loading section 510 and the sample loading section 512 are communicated, the sample loading section 510 and the sample loading section 512 are arranged in a non-straight-through way, the sample loading section 510 is used for accommodating a sample, and the sample loading section 512 is used for allowing the sample to flow out to a counting chip or for allowing trypan blue of a trypan blue liquid outlet mechanism to enter the sample loading hole 51.
In more detail, in this embodiment, the inner wall of the sample application section 510 is divided into a straight section 5121 and a tapered section 5123, the straight section 5121 and the tapered section 5123 are engaged, and the diameter of the cross-sectional profile of the tapered section 5123 is gradually reduced from one end near the straight section 5121 to the other end, and the sample application section 512 is engaged with the side wall of the tapered section 5123. Therefore, a non-straight-through structure is formed, the sample can be kept at the middle position during sample adding, and the sample is prevented from leaking from the bottom of the sample adding hole 51 due to overlarge sample adding operation force.
In this embodiment, the upper and lower ends of the well 51 are provided with sealing gaskets 53. The sample inlet 301 and the liquid outlet 401 of the counting chip are also provided with gasket structures so as to form corresponding sealing pipelines under different working conditions.
Specifically, the porous sampling device 100 further includes a sampling hole cover 55, the motor set further includes a dual-motion motor 39, the dual-motion motor 39 is mounted on the mounting frame 10, an output end of the dual-motion motor 39 is connected with the sampling hole cover 55, the sampling hole cover 55 is located above the sampling position, and the control system can control the dual-motion motor 39 to work, so that the sampling hole cover 55 and the housing 200 seal the sampling hole 51 at the sampling position or the sampling hole cover 55 moves and exposes the sampling hole 51 at the sampling position. Closing the sample aperture cover 55 prevents external contamination when the inspection process is running.
Specifically, the multi-well sample injection device 100 further includes a magnetic position sensor 57 (hall switch device) and a photoelectric encoder 61, where the magnetic position sensor 57 is installed on the mounting frame 10 and is used for positioning the rotation initial position of the multi-well sample injection plate 50, and the photoelectric encoder 61 is installed on the rotation stepping motor 37 and feeds back the rotation angle signal of the multi-well sample injection plate 50 to the control system, and the control system controls the rotation stepping motor 37 to work or stop according to the rotation initial position and the rotation angle signal of the multi-well sample injection plate 50, so that the sample injection hole 51 can move to the working position. The photoelectric encoder 61 feeds back the rotation angle signal of the multi-well loading tray 50 by reading the rotation angle of the rotary stepper motor 37, and the rotation angle of the multi-well loading tray 50 can be known by reading the rotation angle of the output shaft of the rotary stepper motor 37 because the multi-well loading tray 50 and the output shaft of the rotary stepper motor 37 are coaxially arranged. By rotating the initial position and the rotation angle signal, the control system can position each well 51 and accurately move it to the desired working position.
Specifically, the multi-well sample injection device 100 further includes a photoelectric sensor 59, where the photoelectric sensor 59 can detect the position of the multi-well sample injection tray 50 in the up-down direction and can feed back the position of the multi-well sample injection tray 50 in the up-down direction to the control system, and the control system controls the first linear stepper motor 31 to work according to the position of the multi-well sample injection tray 50 in the up-down direction, so that the multi-well sample injection tray 50 is lifted or lowered. Further, the photoelectric sensor 59 is divided into an upper position sensor and a lower position sensor to facilitate detection of the upper and lower positions.
By providing such position detection components as the magnetic position sensor 57, the photoelectric sensor 59 and the photoelectric encoder 61, the control system can better control the operation of the first linear stepper motor 31 and the rotary stepper motor 37 to achieve accurate control of the up-down positions of the well plate 50 and the liquid outlet member 401, as well as accurate control of the rotation angle of the well plate 50 itself.
The principle of this embodiment is:
when multiple samples need to be detected, the sample hole cover 55 can be opened first, so that the sample hole formed in the housing 200 is exposed, and one sample hole 51 is exposed, and after the samples are added, the stepper motor 37 is rotated to operate, so that the next sample hole 51 is exposed, so that the sample adding operation of the next sample can be performed. Other loading holes 51 and the like, after the sample is added, the double-motion motor 39 works, so that the loading hole cover 55 seals the position of the loading hole formed on the housing 200.
Further, the next step of function selection can be performed according to the requirement, for example, cell counting and activity rate detection are performed, and then the sample-adding hole 51 after sample adding is controlled to be operated above the liquid outlet component 401 of the trypan blue liquid outlet mechanism, sample mixing is performed, and then the sample is operated to the sample-adding hole 301 of the counting chip, so that the subsequent sample-adding operation is performed.
If only counting is needed, the loaded sample hole 51 is directly controlled to run to the sample hole 301 of the counting chip, and subsequent operation is carried out.
The implementation of trypan blue mixing, cell counting, and the like can refer to the implementation of the existing cell counter, and the embodiment is not repeated.
In summary, the porous sample injection device 100 of the present invention is configured to allow an operator to perform sample injection of a plurality of samples by combining the porous sample injection plate 50 with a motor unit and a position detection unit, and then to perform subsequent detection of each sample in each sample injection hole 51, without requiring a sample-by-sample detection as in the conventional cytometer. Therefore, sample adding operation time required by detecting the cell sample is saved, the workload of operators is reduced, and the efficiency of the whole detection process is further improved. And the instrument is not required to be kept all the time, so that an operator can conveniently process other transactions in the detection process.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A porous sampling device for the cell counter advances kind, and the cell counter includes casing, count chip, trypan blue play liquid mechanism, and count chip and trypan blue play liquid mechanism all set up in the casing, its characterized in that includes:
the mounting frame is used for being erected in the shell of the cell counter;
the motor group comprises a first linear stepping motor, a second linear stepping motor and a rotary stepping motor;
the disk part of the porous sample adding disk is connected with the output end of the rotary stepping motor, the first linear stepping motor is arranged on the mounting frame, the rotary stepping motor is connected with the output end of the first linear stepping motor and can move up and down under the drive of the first linear stepping motor, and a plurality of sample adding holes which are axially formed in the position, close to the edge, of the porous sample adding disc are distributed;
the vacuum chuck mechanism comprises a chuck support and a vacuum chuck, the chuck support is connected with the output end of the second linear stepping motor, the vacuum chuck is arranged on the chuck support and is positioned above the porous sample adding disc, the position of the vacuum chuck at least corresponds to the position of one sample adding hole, the position of at least one vacuum chuck corresponds to the position of a sample adding hole of the counting chip, and the porous sample adding disc is positioned between the vacuum chuck and the counting chip;
the control system can control the motor group to work or stop;
when in sample adding, a sample is added to one sample adding hole at the sample adding position, the control system controls the rotary stepping motor to work, and the porous sample adding disc rotates to enable the next sample adding hole to be at the sample adding position;
during sample injection, the porous sample adding disc rotates under the drive of the rotary stepping motor, so that the sample adding hole after sample addition moves to the lower part of the vacuum chuck and stops, the control system controls the first linear stepping motor to work so that the rotary stepping motor descends and the porous sample adding disc descends to be attached to the counting chip, the sample adding hole is communicated with the sample adding hole, the control system controls the second linear stepping motor to work so that the vacuum chuck descends and is attached to the porous sample adding disc, the sample adding hole, the vacuum chuck and the counting chip sample adding hole form a sealing pipeline, and a sample in the sample adding hole can enter the counting chip sample adding hole under the action of a pressure source connected with the counting chip;
the sample adding hole is provided with a sample adding section and a sample adding section, the sample adding section is communicated with the sample adding section, the sample adding section and the sample adding section are arranged in a non-straight-through way, the sample adding section is used for accommodating a sample, and the sample adding section is used for allowing the sample to flow out to a counting chip or for allowing trypan blue of a trypan blue liquid outlet mechanism to enter the sample adding hole;
sealing gaskets are arranged at the upper end and the lower end of the sample adding hole.
2. The multi-hole sample injection device of claim 1, wherein the motor group further comprises a third linear stepper motor, the third linear stepper motor is mounted on the mounting frame, and the output end of the third linear stepper motor is in transmission connection with a liquid outlet component of the trypan blue liquid outlet mechanism and can enable the liquid outlet component to move up and down;
when trypan blue mixing is needed, the control system controls the third linear stepping motor to work, so that the liquid outlet component rises, the first linear stepping motor works and enables the porous sample adding disc to descend, the second linear stepping motor works and enables the vacuum chuck to descend, and the liquid outlet component, the sample adding hole and the vacuum chuck form a sealing pipeline.
3. The multi-well sampling device of claim 2, wherein the number of vacuum chucks is two, one vacuum chuck is positioned corresponding to a liquid outlet component of the trypan blue liquid outlet mechanism, and the other vacuum chuck is positioned corresponding to a counting chip sampling hole.
4. The multi-well sampling device of claim 1, wherein the multi-well loading plate has 25 of the loading wells, wherein one of the loading wells is an initial calibration well.
5. The multi-well sample injection device of claim 4, wherein the inner wall of the sample addition section is divided into a straight barrel portion and a tapered barrel portion, the straight barrel portion and the tapered barrel portion are joined, the diameter dimension of the cross-sectional profile of the tapered barrel portion gradually decreases from one end near the straight barrel portion toward the other end, and the sample addition section is joined with the side wall of the tapered barrel portion.
6. The multi-well sampling device of claim 1, further comprising a loading hole cover, wherein the motor assembly further comprises a dual-motion motor, wherein the dual-motion motor is mounted on the mounting frame, an output end of the dual-motion motor is connected with the loading hole cover, the loading hole cover is located above the loading position, and the control system can control the dual-motion motor to work so that the loading hole cover and the housing block the loading hole at the loading position or move the loading hole cover and expose the loading hole at the loading position.
7. The multi-well sample injection device of claim 1, further comprising a magnetic position sensor and a photoelectric encoder, wherein the magnetic position sensor is mounted on the mounting frame and is used for positioning a rotation initial position of the multi-well sample injection plate, the photoelectric encoder is mounted on the rotation stepping motor and feeds back a rotation angle signal of the multi-well sample injection plate to the control system, and the control system controls the rotation stepping motor to work or stop according to the rotation initial position and the rotation angle signal of the multi-well sample injection plate, so that the sample injection hole can move to a working position.
8. The multi-well sampling device of claim 1, further comprising a photoelectric sensor capable of detecting the position of the multi-well loading tray in the up-down direction and feeding back the position of the multi-well loading tray in the up-down direction to the control system, wherein the control system controls the first linear stepping motor to operate according to the position of the multi-well loading tray in the up-down direction so that the multi-well loading tray is lifted or lowered.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811291134.8A CN109061214B (en) | 2018-10-31 | 2018-10-31 | Porous sample injection device |
| PCT/CN2019/114124 WO2020088476A1 (en) | 2018-10-31 | 2019-10-29 | Multi-orifice sample introduction device and multi-orifice sample introduction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811291134.8A CN109061214B (en) | 2018-10-31 | 2018-10-31 | Porous sample injection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109061214A CN109061214A (en) | 2018-12-21 |
| CN109061214B true CN109061214B (en) | 2023-12-19 |
Family
ID=64767983
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811291134.8A Active CN109061214B (en) | 2018-10-31 | 2018-10-31 | Porous sample injection device |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN109061214B (en) |
| WO (1) | WO2020088476A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109060641A (en) * | 2018-10-31 | 2018-12-21 | 江苏卓微生物科技有限公司 | Porous sample introduction cell counter |
| CN109061214B (en) * | 2018-10-31 | 2023-12-19 | 江苏卓微生物科技有限公司 | Porous sample injection device |
| CN114832875A (en) * | 2022-05-28 | 2022-08-02 | 深圳市宝鼎丰科技有限公司 | Integrated micro-fluidic chip equipment |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4085006A (en) * | 1974-11-06 | 1978-04-18 | Isomedics, Incorporated | Automatic cell analyzer method |
| US5395588A (en) * | 1992-12-14 | 1995-03-07 | Becton Dickinson And Company | Control of flow cytometer having vacuum fluidics |
| US5439645A (en) * | 1993-01-25 | 1995-08-08 | Coulter Corporation | Apparatus for automatically, selectively handling multiple, randomly associated hematological samples |
| WO2011026029A1 (en) * | 2009-08-31 | 2011-03-03 | Bio-Rad Laboratories, Inc. | Compact automated cell counter |
| JP2015172509A (en) * | 2014-03-11 | 2015-10-01 | 株式会社東芝 | Clinical examination device |
| CN204874484U (en) * | 2015-04-20 | 2015-12-16 | 南京康芯微健康科技有限公司 | Rare cell automation equipment of catching |
| CN207133167U (en) * | 2017-08-31 | 2018-03-23 | 许金泉 | A kind of novel multi-channel cell counter and multichannel cell count system |
| CN108459172A (en) * | 2018-06-05 | 2018-08-28 | 江苏卓微生物科技有限公司 | Micro-fluidic chip is loaded sealing system and the cytoanalyze comprising it |
| CN108627448A (en) * | 2018-06-05 | 2018-10-09 | 江苏卓微生物科技有限公司 | The method of counting micro particles |
| CN209148708U (en) * | 2018-10-31 | 2019-07-23 | 江苏卓微生物科技有限公司 | Porous sampling device |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3537794A (en) * | 1968-08-14 | 1970-11-03 | Fisher Scientific Co | Apparatus for the automatic analysis of a plurality of blood samples with means for agitation of each sample |
| US4452899A (en) * | 1982-06-10 | 1984-06-05 | Eastman Kodak Company | Method for metering biological fluids |
| JP3972012B2 (en) * | 2003-03-19 | 2007-09-05 | 株式会社日立ハイテクノロジーズ | Sample dispensing mechanism and automatic analyzer equipped with the same |
| JP4664785B2 (en) * | 2005-09-16 | 2011-04-06 | 株式会社Ｅｃｉ | Disc and cell counting and observation apparatus using optical disc using optical disc |
| JP5398620B2 (en) * | 2010-03-30 | 2014-01-29 | 株式会社東芝 | Automatic analyzer |
| JP5781281B2 (en) * | 2010-07-30 | 2015-09-16 | シスメックス株式会社 | Sample processing equipment |
| CN204461973U (en) * | 2015-04-03 | 2015-07-08 | 舟山医院 | Robotization flow cytometer |
| CN104777321B (en) * | 2015-04-16 | 2016-07-06 | 清华大学 | Chemiluminescence immune assay device |
| CN105388314B (en) * | 2015-12-17 | 2017-09-12 | 嘉兴凯实生物科技有限公司 | A kind of full-automatic sample adding instrument |
| CN106153964A (en) * | 2016-08-26 | 2016-11-23 | 梅州康立高科技有限公司 | A kind of electrolyte analyser and the automatically method of sampling |
| US10809178B2 (en) * | 2017-02-22 | 2020-10-20 | JVC Kenwood Corporation | Analysis device and analysis method |
| CN108226550B (en) * | 2018-01-22 | 2023-12-01 | 上海默礼生物医药科技有限公司 | Miniature multi-bin controlled chemiluminescent kit and detection method thereof |
| CN209148506U (en) * | 2018-10-31 | 2019-07-23 | 江苏卓微生物科技有限公司 | Porous sample introduction cell counter |
| CN109061214B (en) * | 2018-10-31 | 2023-12-19 | 江苏卓微生物科技有限公司 | Porous sample injection device |
| CN109060641A (en) * | 2018-10-31 | 2018-12-21 | 江苏卓微生物科技有限公司 | Porous sample introduction cell counter |
-
2018
- 2018-10-31 CN CN201811291134.8A patent/CN109061214B/en active Active
-
2019
- 2019-10-29 WO PCT/CN2019/114124 patent/WO2020088476A1/en active Application Filing
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4085006A (en) * | 1974-11-06 | 1978-04-18 | Isomedics, Incorporated | Automatic cell analyzer method |
| US5395588A (en) * | 1992-12-14 | 1995-03-07 | Becton Dickinson And Company | Control of flow cytometer having vacuum fluidics |
| US5439645A (en) * | 1993-01-25 | 1995-08-08 | Coulter Corporation | Apparatus for automatically, selectively handling multiple, randomly associated hematological samples |
| WO2011026029A1 (en) * | 2009-08-31 | 2011-03-03 | Bio-Rad Laboratories, Inc. | Compact automated cell counter |
| JP2015172509A (en) * | 2014-03-11 | 2015-10-01 | 株式会社東芝 | Clinical examination device |
| CN204874484U (en) * | 2015-04-20 | 2015-12-16 | 南京康芯微健康科技有限公司 | Rare cell automation equipment of catching |
| CN207133167U (en) * | 2017-08-31 | 2018-03-23 | 许金泉 | A kind of novel multi-channel cell counter and multichannel cell count system |
| CN108459172A (en) * | 2018-06-05 | 2018-08-28 | 江苏卓微生物科技有限公司 | Micro-fluidic chip is loaded sealing system and the cytoanalyze comprising it |
| CN108627448A (en) * | 2018-06-05 | 2018-10-09 | 江苏卓微生物科技有限公司 | The method of counting micro particles |
| CN209148708U (en) * | 2018-10-31 | 2019-07-23 | 江苏卓微生物科技有限公司 | Porous sampling device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109061214A (en) | 2018-12-21 |
| WO2020088476A1 (en) | 2020-05-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109061214B (en) | Porous sample injection device | |
| WO2020088474A1 (en) | Porous sample feeding cell counter and counting method | |
| US20040096368A1 (en) | Assay systems and components | |
| US6937955B2 (en) | Method for automatic alignment of metering system for a clinical analyzer | |
| EP3629027B1 (en) | Chemiluminescence detection equipment and its operation method | |
| KR20010020145A (en) | Analysis System | |
| CN109541244A (en) | A kind of iodine element automatic detector and its control method | |
| EP0753735A2 (en) | Specimen testing apparatus | |
| EP2659172B1 (en) | Rotary shear valve with tree-point stator seating | |
| MXPA96002282A (en) | Luminime | |
| JPH04115136A (en) | Particle measuring apparatus | |
| CN209148708U (en) | Porous sampling device | |
| CN209148506U (en) | Porous sample introduction cell counter | |
| EP1368635B1 (en) | Apparatus and method for processing and testing a biological specimen | |
| KR101970790B1 (en) | Method and apparatus with improved accuracy | |
| US11420197B2 (en) | Apparatus and method for mixing fluid or media by vibrating a pipette using nonconcentric masses | |
| KR102133908B1 (en) | Cartridge for Automatic immunoassay diagnostic system | |
| CN216525867U (en) | POCT blood cell analyzer | |
| KR20050016916A (en) | Improved assay systems and components |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |