CN109060910A - A kind of preparation method of isopropyl methoxalamine molecular engram sensor - Google Patents
A kind of preparation method of isopropyl methoxalamine molecular engram sensor Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
Abstract
The invention discloses a kind of preparation methods of isopropyl methoxalamine molecular engram sensor, it is characterized in that, in the reactor, it is added by following composition mass percentage concentration, ethyl alcohol: 74 ~ 80%, N- ethyl-2-aminomethylpentazaneand: 6 ~ 10%, citraconic acid: 6 ~ 10%, isopropyl methoxalamine: 2 ~ 4%, diisocyanate: 0.5 ~ 1.5%, ammonium persulfate: 3 ~ 5%, the sum of each component content is absolutely, stirring and dissolving, logical nitrogen deoxygenation 10min, anaerobic atmosphere, 60 ± 2 DEG C are stirred to react 10 ~ 12h, the product ethyl alcohol that will be obtained: acetic acid volume ratio is that 8:1 mixed solution impregnates 10h, repeatedly washing, remove template molecule, it is dry, up to isopropyl methoxalamine molecularly imprinted polymer；The sensor has high sensitivity and selectivity.With high sensitivity, specificity is good, detection is quick, can Reusability.
Description
Technical field
Applied technical field the present invention relates to a kind of preparation method of molecular engram sensor and quickly is detected, especially
It is related to a kind of preparation method of isopropyl methoxalamine molecular engram sensor, for the isopropyl methoxalamine in test sample.
Background technique
Isopropyl methoxalamine sterling is colourless liquid, and chemical name is 2- methyl -6- ethyl-N- (1- methyl -2- methoxy second
Base)-N- ChloroacetYl aniline；(20 DEG C) of specific gravity be 1.12,100 DEG C of boiling point, 110~180 DEG C of flash-point.Solubility in water
530mg/kg (20 DEG C), can with most of immiscible organic solvents, storage at normal temperature stationary phase 2 years or more.72% cream of isopropyl methoxalamine
Oil is brown color liquid, and emulsifiability is good, can mutually mix with many herbicides, storage at normal temperature stability 2 years or more.Isopropyl first
Careless amine applies to dry crop, vegetable crop and orchard, nursery and uses, and can prevent and kill off herba eleusines indicae, herba digitariae, herba setariae viridis, cotton grass etc. one
Broadleaf weeds and the cyperus iria, chufa such as year raw gramineae weed and three-coloured amaranth, purslane.By national standard agriculture chemical registration poison
What the assessment processes such as pharmacologic test procedure regulation and chemistry security toxicity carried out presses acute toxicity evaluation criterion isopropyl methoxalamine
Acute oral, percutaneous toxicity test result belong to low toxicity level, female rats acute oral LD50It is big for 2330mg/ kg. male
Mouse acute oral LD50It is more sensitive than male mouse for the medicine female mice for 3160mg/kg..Skin and eye irritant test result are light
Spend irritation.Skin anaphylactic test this medicine of result is to skin without sensitization, genetoxic: sperm abnormality test and micronucleus examination
Testing with Salmonella reversion test is negative findings.Isopropyl methoxalamine is still safer as herbicide in summary.China's regulation,
The MRL value of isopropyl methoxalamine is 0.1 mg/kg. in corn
Currently, the method for detection isopropyl methoxalamine mainly has a high performance liquid chromatography, liquid chromatography-mass spectrography, these methods it is accurate
Degree, which is subject to certain restrictions, and instrument is somewhat expensive needs professional to operate, and detection cycle is long, also limits its application.In addition,
Since residual quantity is relatively low on the plants such as vegetables, coexisting substances interfere with each other for isopropyl methoxalamine, isopropyl methoxalamine is accurately detected
It is highly difficult.Therefore, finding a kind of good selectivity, high sensitivity, the method for the detection isopropyl methoxalamine easy to operate being used for has
Important use value.
Molecular imprinting technology is one of the main method of high selectivity material that current exploitation has molecular recognition function, it
It is to be polymerize after removing template molecule in molecular engram by forming a highly cross-linked rigid macromolecule around template molecule
The recognition site with binding ability is left in the network structure of object, and one kind of high selection recognition performance is shown to template molecule
Technology.This technology imitates precordainment with its structure and specific recognition has been to be concerned by more and more people, and has been used successfully to solid phase
Extraction or the fields such as micro- Solid Phase Extraction, affinity chromatography or Capillary Electrophoresis and sensor.
According to the molecular engram sensor of this technology preparation, it is applied to Pharmaceutical Analysis, environmental protection and life science
In play a very important role.Functional molecular is modified on electrode in a suitable manner, the selective good, high sensitivity of preparation,
There is the reproducible electrochemical sensor of certain service life to become analysis science worker and makes great efforts the project explored.But it is traditional
Trace film thickness prepared by immunoblot method is difficult to control, and high-crosslinking-degree makes electron transmission speed and low-response, Monitoring lower-cut
Height influences application of the molecular imprinting technology in electrochemical sensor.Therefore, it establishes a kind of sensitive, quick, easy, specific
The detection method that high, reproducible economy uses, to the isopropyl methoxalamine content accurate quantitative analysis in food, drug, biological sample
It measures very necessary.
The purpose of the present invention is combining molecular engram with electrochemical sensor, a kind of isopropyl methoxalamine molecule is provided
The preparation method of trace sensor, mainly using N- ethyl-2-aminomethylpentazaneand and citraconic acid as function monomer, diisocyanate
Ester is crosslinking agent, and ammonium persulfate is initiator, and isopropyl methoxalamine is template, glassy carbon electrode surface by graphene oxide, receive
The modification of rice gold particle, improves the sensitivity of sensor, is passed using drop-coating building isopropyl methoxalamine molecular imprinting electrochemical
Sensor.
Summary of the invention
Instrument and reagent
CHI660B electrochemical workstation (Shanghai Chen Hua instrument company), experiment use three-electrode system: platinum electrode is auxiliary electricity
Pole, Ag/AgCl are reference electrode (SCE), and glass-carbon electrode (GCE) is working electrode；KQ-250E type ultrasonic cleaner (female peak
Ultrasonic instrument Co., Ltd).
Graphene oxide；N- ethyl-2-aminomethylpentazaneand, citraconic acid, diisocyanate, ammonium persulfate, isopropyl first grass
Amine, methanol, ethyl alcohol, gold chloride, chitosan, acetic acid, citric acid, phosphate buffer solution；Agents useful for same is that analysis is pure, and experiment is used
Water is secondary distilled water.
A kind of preparation method of isopropyl methoxalamine molecular engram sensor, is characterized in that, this method is walked with following technique
It is rapid:
(1) prepared by nano gold/graphene oxide decorating liquid: in the reactor, it is added by following composition mass ratio, deionized water:
85 ~ 92%, gold chloride: 1.0 ~ 3.0%, graphene oxide: 2 ~ 6%, ultrasound 20min at room temperature are uniformly dispersed, add citric acid:
3 ~ 8%, the sum of each component content is absolutely, to be heated to 55 ± 2 DEG C, be stirred to react 40min, obtain nanogold/graphite oxide
Alkene decorating liquid；
(2) prepared by nano gold/graphene oxide modified electrode: glass-carbon electrode is successively carried out table with 0.3 μm, 0.01 μm of polishing powder
Face polishing, is then cleaned by ultrasonic with secondary distilled water, ethanol washing respectively, is dried up, is immersed in nanogold/oxidation in glass-carbon electrode
30min in graphene modified liquid, taking-up are placed under infrared lamp, modify electricity after the dry solvent that volatilizees to get nano gold/graphene oxide
Pole；
(3) preparation of isopropyl methoxalamine molecularly imprinted polymer: in the reactor, being added by following composition mass percentage concentration,
Ethyl alcohol: 74 ~ 80%, N- ethyl-2-aminomethylpentazaneand: 6 ~ 10%, citraconic acid: 6 ~ 10%, isopropyl methoxalamine: 2 ~ 4%, diisocyanate
Ester: 0.5 ~ 1.5%, ammonium persulfate: 3 ~ 5%, the sum of each component content is that absolutely, stirring and dissolving leads to nitrogen deoxygenation 10min,
Anaerobic atmosphere, 60 ± 2 DEG C are stirred to react 10 ~ 12h, the product ethyl alcohol that will be obtained: acetic acid volume ratio is the immersion of 8:1 mixed solution
10h is repeatedly washed, and removes template molecule, dry to get isopropyl methoxalamine molecularly imprinted polymer；
(4) preparation of isopropyl methoxalamine molecular engram sensor: suitable isopropyl methoxalamine molecularly imprinted polymer is taken to be scattered in
In 1% chitosan acetic acid solution, the isopropyl methoxalamine imprinted polymer solution of 18g/L is made；Then by above-mentioned solution
16 ~ 20 μ L are added drop-wise to the nano gold/graphene oxide modified electrode of step (2) preparation, are placed under infrared lamp, volatilize dry solvent
Afterwards to get isopropyl methoxalamine molecular engram sensor.
The mass ratio of the gold chloride and graphene oxide is between 1:2 ~ 2.5 in step (1).
The mass ratio of the gold chloride and citric acid is between 1:3 ~ 3.5 in step (1).
The N- ethyl-2-aminomethylpentazaneand and citraconic acid mole is 1:1 in step (3).
The anaerobic atmosphere is that polymerization process is passed through always nitrogen in step (3).
Advantages of the present invention and effect are:
The present invention combines engram technology, LBL self-assembly method and drop-coating, in nano gold/graphene oxide modification glass carbon electricity
Pole surface successfully has developed a kind of isopropyl methoxalamine trace electrochemical sensor with specific selectivity, by with do not use
The response of the molecular engram electrode of nano gold/graphene oxide modification is compared, isopropyl methoxalamine molecule prepared by the present invention
The response of trace sensor greatly improves, good, the high sensitivity of selectivity.The trace sensor shows isopropyl methoxalamine higher
Compatibility and selectivity, the concentration of response current and isopropyl methoxalamine is 4.0 × 10-8~4.0×10-6It is within the scope of mol/L
Good linear relationship, detection are limited to 1.68 × 10-9Mol/L passes isopropyl methoxalamine molecular imprinting electrochemical prepared by the present invention
Sensor is used successfully in sample in the detection of isopropyl methoxalamine, and the rate of recovery is between 96.22 ~ 103.81%, therefore prepared by the present invention
Isopropyl methoxalamine molecular engram sensor can be widely applied to the related fieldss such as chemical industry, pesticide residue, biology, environmental protection tests.
Specific embodiment
Embodiment 1
1) prepared by nano gold/graphene oxide decorating liquid: it in the reactor, is separately added into, 88 mL of deionized water, gold chloride:
2.0g, graphene oxide: 5.0g, ultrasound 20min: the 5.0g that is uniformly dispersed, adds citric acid are heated to 55 ± 2 at room temperature
DEG C, it is stirred to react 40min, obtains nano gold/graphene oxide decorating liquid；
(2) prepared by nano gold/graphene oxide modified electrode: glass-carbon electrode is successively carried out table with 0.3 μm, 0.01 μm of polishing powder
Face polishing, is then cleaned by ultrasonic with secondary distilled water, ethanol washing respectively, is dried up, is immersed in nanogold/oxidation in glass-carbon electrode
30min in graphene modified liquid, taking-up are placed under infrared lamp, modify electricity after the dry solvent that volatilizees to get nano gold/graphene oxide
Pole；
(3) it the preparation of isopropyl methoxalamine molecularly imprinted polymer: in the reactor, is separately added into, ethyl alcohol: 98 mL, N- ethyl-
2- aminomethyl pyrrolidine: 8.0g, citraconic acid: 8.0g, isopropyl methoxalamine 3.0g, diisocyanate: 1.0g, ammonium persulfate: 3.0g,
Stirring and dissolving leads to nitrogen deoxygenation 10min, and anaerobic atmosphere, 60 ± 2 DEG C are stirred to react 11h, the product ethyl alcohol that will be obtained: acetic acid
Volume ratio is that 8:1 mixed solution impregnates 10h, is repeatedly washed, and template molecule is removed, dry to get isopropyl methoxalamine molecular engram
Polymer；
(4) preparation of isopropyl methoxalamine molecular engram sensor: suitable isopropyl methoxalamine molecularly imprinted polymer is taken to be scattered in
In 1% chitosan acetic acid solution, the isopropyl methoxalamine imprinted polymer solution of 18g/L is made；Then by above-mentioned solution
17 μ L are added drop-wise to the nano gold/graphene oxide modified electrode of step (2) preparation, are placed under infrared lamp, after the dry solvent that volatilizees, i.e.,
Obtain isopropyl methoxalamine molecular engram sensor.
Embodiment 2
1) prepared by nano gold/graphene oxide decorating liquid: it in the reactor, is separately added into, 92 mL of deionized water, gold chloride:
1.0g, graphene oxide: 3.0g, ultrasound 20min: the 4.0g that is uniformly dispersed, adds citric acid are heated to 55 ± 2 at room temperature
DEG C, it is stirred to react 40min, obtains nano gold/graphene oxide decorating liquid；
(2) prepared by nano gold/graphene oxide modified electrode: glass-carbon electrode is successively carried out table with 0.3 μm, 0.01 μm of polishing powder
Face polishing, is then cleaned by ultrasonic with secondary distilled water, ethanol washing respectively, is dried up, is immersed in nanogold/oxidation in glass-carbon electrode
30min in graphene modified liquid, taking-up are placed under infrared lamp, modify electricity after the dry solvent that volatilizees to get nano gold/graphene oxide
Pole；
(3) it the preparation of isopropyl methoxalamine molecularly imprinted polymer: in the reactor, is separately added into, ethyl alcohol: 101 mL, N- ethyl-
2- aminomethyl pyrrolidine: 6.0g, citraconic acid: 6.0g, isopropyl methoxalamine 2.5g, diisocyanate: 0.5g, ammonium persulfate: 5.0g,
Stirring and dissolving leads to nitrogen deoxygenation 10min, and anaerobic atmosphere, 60 ± 2 DEG C are stirred to react 10h, the product ethyl alcohol that will be obtained: acetic acid
Volume ratio is that 8:1 mixed solution impregnates 10h, is repeatedly washed, and template molecule is removed, dry to get isopropyl methoxalamine molecular engram
Polymer；
(4) preparation of isopropyl methoxalamine molecular engram sensor: suitable isopropyl methoxalamine molecularly imprinted polymer is taken to be scattered in
In 1% chitosan acetic acid solution, the isopropyl methoxalamine imprinted polymer solution of 18g/L is made；Then by above-mentioned solution
18 μ L are added drop-wise to the nano gold/graphene oxide modified electrode of step (2) preparation, are placed under infrared lamp, after the dry solvent that volatilizees, i.e.,
Obtain isopropyl methoxalamine molecular engram sensor.
Embodiment 3
1) prepared by nano gold/graphene oxide decorating liquid: it in the reactor, is separately added into, 85 mL of deionized water, gold chloride:
3.0g, graphene oxide: 6.0g, ultrasound 20min: the 6.0g that is uniformly dispersed, adds citric acid are heated to 55 ± 2 at room temperature
DEG C, it is stirred to react 40min, obtains nano gold/graphene oxide decorating liquid；
(2) prepared by nano gold/graphene oxide modified electrode: glass-carbon electrode is successively carried out table with 0.3 μm, 0.01 μm of polishing powder
Face polishing, is then cleaned by ultrasonic with secondary distilled water, ethanol washing respectively, is dried up, is immersed in nanogold/oxidation in glass-carbon electrode
30min in graphene modified liquid, taking-up are placed under infrared lamp, modify electricity after the dry solvent that volatilizees to get nano gold/graphene oxide
Pole；
(3) it the preparation of isopropyl methoxalamine molecularly imprinted polymer: in the reactor, is separately added into, ethyl alcohol: 94 mL, N- ethyl-
2- aminomethyl pyrrolidine: 10g, citraconic acid: 10g, isopropyl methoxalamine 2.0g, diisocyanate: 1.5g, ammonium persulfate: 3.5g is stirred
Dissolution is mixed, leads to nitrogen deoxygenation 10min, anaerobic atmosphere, 60 ± 2 DEG C are stirred to react 12h, the product ethyl alcohol that will be obtained: acetic acid body
Product is multiple to wash than being that 8:1 mixed solution impregnates 10h, removes template molecule, dry poly- to get isopropyl methoxalamine molecular engram
Close object；
(4) preparation of isopropyl methoxalamine molecular engram sensor: suitable isopropyl methoxalamine molecularly imprinted polymer is taken to be scattered in
In 1% chitosan acetic acid solution, the isopropyl methoxalamine imprinted polymer solution of 18g/L is made；Then by above-mentioned solution
16 μ L are added drop-wise to the nano gold/graphene oxide modified electrode of step (2) preparation, are placed under infrared lamp, after the dry solvent that volatilizees, i.e.,
Obtain isopropyl methoxalamine molecular engram sensor.
Embodiment 4
1) prepared by nano gold/graphene oxide decorating liquid: it in the reactor, is separately added into, deionized water 89mL, gold chloride:
1.0g, graphene oxide: 2.0g, ultrasound 20min: the 8.0g that is uniformly dispersed, adds citric acid are heated to 55 ± 2 at room temperature
DEG C, it is stirred to react 40min, obtains nano gold/graphene oxide decorating liquid；
(2) prepared by nano gold/graphene oxide modified electrode: glass-carbon electrode is successively carried out table with 0.3 μm, 0.01 μm of polishing powder
Face polishing, is then cleaned by ultrasonic with secondary distilled water, ethanol washing respectively, is dried up, is immersed in nanogold/oxidation in glass-carbon electrode
30min in graphene modified liquid, taking-up are placed under infrared lamp, modify electricity after the dry solvent that volatilizees to get nano gold/graphene oxide
Pole；
(3) it the preparation of isopropyl methoxalamine molecularly imprinted polymer: in the reactor, is separately added into, ethyl alcohol: 95mL, N- ethyl -2-
Aminomethyl pyrrolidine: 9.0g, citraconic acid: 7.0g, isopropyl methoxalamine 4.0g, diisocyanate: 0.8g, ammonium persulfate: 4.0g is stirred
Dissolution is mixed, leads to nitrogen deoxygenation 10min, anaerobic atmosphere, 60 ± 2 DEG C are stirred to react 11h, the product ethyl alcohol that will be obtained: acetic acid body
Product is multiple to wash than being that 8:1 mixed solution impregnates 10h, removes template molecule, dry poly- to get isopropyl methoxalamine molecular engram
Close object；
(4) preparation of isopropyl methoxalamine molecular engram sensor: suitable isopropyl methoxalamine molecularly imprinted polymer is taken to be scattered in
In 1% chitosan acetic acid solution, the isopropyl methoxalamine imprinted polymer solution of 18g/L is made；Then by above-mentioned solution
20 μ L are added drop-wise to the nano gold/graphene oxide modified electrode of step (2) preparation, are placed under infrared lamp, after the dry solvent that volatilizees, i.e.,
Obtain isopropyl methoxalamine molecular engram sensor.
Embodiment 5
By isopropyl methoxalamine molecular engram sensor prepared by above-described embodiment 1 ~ 4, for the detection of isopropyl methoxalamine, step
It is as follows:
(1) standard solution is prepared: preparing the isopropyl methoxalamine standard solution of one group of various concentration including blank standard specimen, bottom
Liquid is the phosphate buffer solution of pH6.5；
(2) working curve is drawn: being reference electrode by Ag/AgCl, platinum electrode is auxiliary electrode, isopropyl first prepared by the present invention
Careless amine molecule trace sensor is that working electrode forms three-electrode system, CHI660B electrochemical workstation is connected, in K3[Fe
(CN)6] in solution, detected in -0.10 ~ 0.7V potential range using cyclic voltammetry, the response current of blank standard specimen
It is denoted asI 0 , the response current of the isopropyl methoxalamine standard solution containing various concentration isI i , response current reduce difference be△I=I 0 -I i ,△IWith the mass concentration of isopropyl methoxalamine standard solutioncBetween it is in a linear relationship, draw△I ~cWorking curve；
(3) detection of isopropyl methoxalamine: the isopropyl methoxalamine standard solution in step (1) is replaced with sample to be tested, according to step
(2) method is detected, according to response the difference of current reduction△IAnd working curve, obtain isopropyl first grass in sample to be tested
The content of amine；
The K3[Fe(CN)6] solution concentration be 4.0mmol/L；
The concentration of the phosphate buffer solution of the pH6.5 is in 0.1 mol/L.
Claims (5)
1. a kind of preparation method of isopropyl methoxalamine molecular engram sensor, is characterized in that, this method has following processing step:
(1) prepared by nano gold/graphene oxide decorating liquid: in the reactor, it is added by following composition mass ratio, deionized water:
85 ~ 92%, gold chloride: 1.0 ~ 3.0%, graphene oxide: 2 ~ 6%, ultrasound 20min at room temperature are uniformly dispersed, add citric acid:
3 ~ 8%, the sum of each component content is absolutely, to be heated to 55 ± 2 DEG C, be stirred to react 40min, obtain nanogold/graphite oxide
Alkene decorating liquid；
(2) prepared by nano gold/graphene oxide modified electrode: glass-carbon electrode is successively carried out table with 0.3 μm, 0.01 μm of polishing powder
Face polishing, is then cleaned by ultrasonic with secondary distilled water, ethanol washing respectively, is dried up, is immersed in nanogold/oxidation in glass-carbon electrode
30min in graphene modified liquid, taking-up are placed under infrared lamp, modify electricity after the dry solvent that volatilizees to get nano gold/graphene oxide
Pole；
(3) preparation of isopropyl methoxalamine molecularly imprinted polymer: in the reactor, being added by following composition mass percentage concentration,
Ethyl alcohol: 74 ~ 80%, N- ethyl-2-aminomethylpentazaneand: 6 ~ 10%, citraconic acid: 6 ~ 10%, isopropyl methoxalamine: 2 ~ 4%, diisocyanate
Ester: 0.5 ~ 1.5%, ammonium persulfate: 3 ~ 5%, the sum of each component content is that absolutely, stirring and dissolving leads to nitrogen deoxygenation 10min,
Anaerobic atmosphere, 60 ± 2 DEG C are stirred to react 10 ~ 12h, the product ethyl alcohol that will be obtained: acetic acid volume ratio is the immersion of 8:1 mixed solution
10h is repeatedly washed, and removes template molecule, dry to get isopropyl methoxalamine molecularly imprinted polymer；
(4) preparation of isopropyl methoxalamine molecular engram sensor: suitable isopropyl methoxalamine molecularly imprinted polymer is taken to be scattered in
In 1% chitosan acetic acid solution, the isopropyl methoxalamine imprinted polymer solution of 18g/L is made；Then by above-mentioned solution
16 ~ 20 μ L are added drop-wise to the nano gold/graphene oxide modified electrode of step (2) preparation, are placed under infrared lamp, volatilize dry solvent
Afterwards to get isopropyl methoxalamine molecular engram sensor.
2. a kind of preparation method of isopropyl methoxalamine molecular engram sensor according to claim 1, is characterized in that, in step
Suddenly the mass ratio of gold chloride described in (1) and graphene oxide is between 1:2 ~ 2.5.
3. a kind of preparation method of isopropyl methoxalamine molecular engram sensor according to claim 1, is characterized in that, in step
Suddenly the mass ratio of gold chloride described in (1) and citric acid is between 1:3 ~ 3.5.
4. a kind of preparation method of isopropyl methoxalamine molecular engram sensor according to claim 1, is characterized in that, in step
Suddenly N- ethyl-2-aminomethylpentazaneand described in (3) and citraconic acid mole is 1:1.
5. a kind of preparation method of isopropyl methoxalamine molecular engram sensor according to claim 1, is characterized in that, in step
Suddenly anaerobic atmosphere described in (3) is that polymerization process is passed through always nitrogen.
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Cited By (2)
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| CN111662410A (en) * | 2020-07-06 | 2020-09-15 | 华南农业大学 | Sandwich-structure molecularly imprinted SERS substrate and preparation method and application thereof |
| CN111879833A (en) * | 2020-08-03 | 2020-11-03 | 盐城工学院 | Preparation method of metolachlor molecularly imprinted electrochemical sensor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662410A (en) * | 2020-07-06 | 2020-09-15 | 华南农业大学 | Sandwich-structure molecularly imprinted SERS substrate and preparation method and application thereof |
| CN111879833A (en) * | 2020-08-03 | 2020-11-03 | 盐城工学院 | Preparation method of metolachlor molecularly imprinted electrochemical sensor |
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