CN109052656A - A kind of activity powdered carbon-degradation bacteria composition and groundwater remediation technology - Google Patents
A kind of activity powdered carbon-degradation bacteria composition and groundwater remediation technology Download PDFInfo
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- CN109052656A CN109052656A CN201810772402.1A CN201810772402A CN109052656A CN 109052656 A CN109052656 A CN 109052656A CN 201810772402 A CN201810772402 A CN 201810772402A CN 109052656 A CN109052656 A CN 109052656A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
- C02F2003/003—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms using activated carbon or the like
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/32—Hydrocarbons, e.g. oil
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/04—Surfactants, used as part of a formulation or alone
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Abstract
The invention discloses a kind of active powdered carbon-degradation bacteria composition and groundwater remediation technology, active powdered carbon-degradation bacteria composition includes: efficient degradation strain, active powdered carbon, Silane coupling agent KH550, neopelex, Triton X-100, polysiloxanes, Sodium Polyacrylate and deionized water.The present invention is combined by active carbon with microbial technique, activated carbon adsorption petroleum hydrocarbon latter aspect rapidly reduces the concentration of petroleum hydrocarbon in water, on the other hand, be adsorbed on activated carbon surface high concentration petroleum hydrocarbon can be attached to again on the surface Institute of Micro-biology degradation, the sustainable circulation removal mechanisms at work that an absorption-degradation-is adsorbed again-degrades again is formed in this way, it is adsorbed relative to simple activity charcoal, or simple microbial degradation, it is fast with speed is removed to the petroleum hydrocarbon in water, it can thoroughly degrade, and adsorb and the reproducible advantage of degradation capability again.
Description
Technical field
The present invention relates to field of underground water pollution repair, specially one kind can repair gas station's bavin Water Oil Or Gas oil leak
The active powdered carbon of caused pollution of underground aquifers-degradation bacteria combines underground water recovery technique.
Background technique
Blowout inevitably occurs in its exploitation, processing, storage and transportational process, leaks and leads to petroleum for petroleum
The spilling and discharge of hydro carbons, hence into environment, with surface water seapage polluted underground water.Oil product is in addition to fatty hydrocarbon and virtue
Other than hydrocarbon, also containing considerable phenol and organochlorine etc..Studies have shown that many organic pollutants have carcinogenic, teratogenesis, cause
" three-induced effect " of mutation, seriously affects human health.Moreover, most organic pollutants are in soil and groundwater environment
In be difficult to remove by natural degradation process, by long-term existence and accumulate.In order to protect groundwater environment, reinforce to petroleum
The reparation of polluted underground water is particularly important with improvement.
With the appearance of " national pollution prevention plan (2011-2020) ", underground water pollution repair is
Gradually start.The groundwater remediation in more than 30 years of developed country experience have shown that, it is the huge engineering of investment.Carry out in China
The initial stage of extensive groundwater remediation, accelerated development it is quick, effective, cheap be innovatively lauched recovery technique, be beneficial to
China plays newcoming advantage, and effective reparation of underground water pollution is completed with less investment.
For there are many petroleum pollution restorative procedures, underground water original position microorganism remediation technology have remediation efficiency it is high,
It the advantages that small, without secondary pollution to ambient enviroment disturbance, is paid close attention in recent years by people.But there is also shortcomings for it: cannot
It degrades all organic pollutants；When medium permeability is low, the growth of microorganism easily causes clogging；Organic pollutant
When concentration is too low, it is not able to satisfy requirement of the microorganism growth to carbon source；By petroleum component and physicochemical property, environmental condition and micro-
The restriction of many factors such as biocoene composition, degradation efficiency is low, and degradation time is long etc..To overcome these disadvantages, have to be added
Strong microorganism remediation technology is used in combination with other recovery techniques.
Active carbon is using biomass as raw material, through high temperature carbonization, steam activation, then through ultra-fine grinding techniques grinding prepare it is micro-
Scale particles, have microcellular structure abundant, and powerful specific surface area is good to the adsorption effect of petroleum hydrocarbon.By its adsorptivity
Ground Water Oil Pollution is repaired in conjunction with the degradation capability of microorganism, will be a weight of underground water polluted by petroleum hydrocarbon recovery technique
Quantum jump.
Summary of the invention
The present invention in order to solve the problems in the existing technology, under proposing a kind of active powdered carbon-degradation bacteria jointly
Water recovery technique, by sample acquisition, Spawn incubation, activated carbon modified, liquid for plugging and obstruct liquid preparation and injection in situ come
Realize the recovery technique to underground water in petroleum hydrocarbon contaminated water-bearing layer.
The technical solution adopted by the present invention are as follows: a kind of activity powdered carbon-degradation bacteria composition, active powdered carbon-degradation bacteria combination
Object includes: efficient degradation strain, active powdered carbon, Silane coupling agent KH550, neopelex, Triton X-100, poly- silicon
Oxygen alkane, Sodium Polyacrylate and deionized water.
Further, comprising the following steps:
A, Silane coupling agent KH550, neopelex, Triton X-100, polysiloxanes are added to deionized water
In A lapping liquid is made, wherein the molecular weight of polysiloxanes be 800-1000；
B, by Sodium Polyacrylate, polysiloxanes is added in deionized water, and B lapping liquid is made；The wherein phase of Sodium Polyacrylate
It is 40,000,000 to molecular weight；
C, it after active powdered carbon and A lapping liquid being mixed, is added in bidirectional rotation ball mill and grinds, then to ball mill
Middle addition B lapping liquid, then grind, then by the mixed liquor high speed centrifugation after grinding, supernatant is outwelled, dries, that is, reparation is made
Agent；
D, by efficient degradation strain in oil-containing culture medium amplification cultivation, by the efficient degradation strain after renovation agent, amplification
In mass ratio it is 1:2~5:100 mixing with minimal medium, is stirred with impeller-type stirrer, make renovation agent and strain in water
In it is evenly dispersed, liquid for plugging is made；
E, by after renovation agent, amplification efficient degradation strain and minimal medium be in mass ratio 1:20~50:2000
Mixing, is stirred with impeller-type stirrer, keeps renovation agent and strain evenly dispersed in water, barrier liquid is made；
F, leakage point establishes blotter coat: using the direct drilling machine with medicament ejector head in leakage point along direction of groundwater flow first
It carries out directly pushing away drilling within the scope of 2~5 meters of downstream direction, injector head connects slush pump, and slush pump is drawn from liquid for plugging holding vessel and sealed
Injector head is mentioned 90cm upwards and carries out second of injection again, until eject position and leakage point by stifled liquid after completing primary injection
Height maintains an equal level；
G, points of contamination establishes hydrodynamic seepage pressure (IPRB) in situ: using the direct drilling machine with medicament ejector head in dirt first
Dye point carries out directly pushing away drilling along subsurface flow downstream 2~5 meters, injector head connection slush pump, and slush pump is from envelope
Stifled liquid holding vessel draws liquid for plugging, after completing primary injection, injector head is mentioned to 90cm upwards and carries out second of injection again, until spray
Position is penetrated to reach apart from earth's surface 2m；It completes to inject for the first time, forms first injector well；
H, step f-g is repeated, the note of second mouthful of injector well is carried out on 3 meters of remote positions of first injector well again
It penetrates.
Further, the efficient degradation strain is efficient diesel or gasoline degradation strain, screening process are as follows: acquisition quilt
The oil-containing water sample of diesel oil or gasoline contamination, dilution, is coated on oil-containing plating medium and turns out bacterium colony, scribing line separation, purifying,
Strain after purification is tamed 3-4 times repeatedly on fresh oil-containing culture medium flat plate, obtains efficient diesel or gasoline degradation strain.
Further, the active powdered carbon is one of cocoanut active charcoal, charcoal or bamboo charcoal, the ratio of the activity powdered carbon
Surface area is greater than 1000m2/ g, partial size are less than 0.5cm.
Further, the component of the step d and the minimal medium in step f includes: by ratio of weight and the number of copies
KH2PO41 part, K2HPO41 part, NH4NO31 part, MgSO40.5 part, CaCl20.02 part, FeSO47H20.01 part of O and deionization
1000 parts of water, and the pH of minimal medium is 7.0；The minimal medium first passes around culture, and temperature is 30~40 DEG C,
Incubation time is 3~5 days, then cooling after 121 DEG C, 30min high pressure sterilization again to use.
Further, the oil-containing culture medium in the step a, step d and step f includes diesel oil/vapour by ratio of weight and the number of copies
Oil 1 part and 1000 parts of inorganic salts culture solution, the plate oil-containing culture medium are to be made after oil-containing culture medium cooled and solidified；It is described to contain
Oily culture medium first passes around culture, and temperature is 30~40 DEG C, and incubation time is 3~5 days, then again in 121 DEG C, 30min high pressure
It is cooling after sterilizing to use.
Further, the mass ratio of active powdered carbon, A lapping liquid and B lapping liquid is 2:1:2 in the step c；It is described again
The time of grinding is 48~72h, and ultracentrifugal revolving speed is 8000~10000r/min, and centrifugation time is 30min or more.
Further, Step d efficient degradation strain amplification cultivation in oil-containing culture medium, the strain quantity after amplification
Concentration is 108~1012A/ml.
Further, the injection pressure of slush pump is greater than 2.5MPa in the f step, and flow is greater than 50L/ minutes, blocks
Liquid stores tankage size and is not less than 1m3；When injection injection, injector head will be pressed onto most deep projected depth, reach pollution water-bearing layer as far as possible
Bottom, or at least below 1 meter of level of ground water or more；Depth is that the depth of oil leak is found when exploring early period when injection, injects well head
Overpressure is not less than 15 atmospheric pressure, and injection flow is initially held at 20L/s or more, and each the amount of injection is 1.5~2.0m3。
Further, slush pump injection pressure is greater than 2.5MPa in the g step, and flow is greater than 20L/ minutes, obstructs liquid
It stores tankage size and is not less than 1m3；When injection injection, injector head is pressed onto pollution water-bearing layer bottom first, if directly pushing away drilling machine probing
Depth cannot reach water-bearing layer bottom, then do not use and directly push away drilling well injection mode, be changed to rotary drilling and construct injection well injection；Spray
Well head overpressure and initial injection flow are consistent with when establishing blotter coat when penetrating, and each the amount of injection is 1.0m3More than.
What the present invention obtained has the beneficial effect that 1. can inject the micro mist in water-bearing layer by the way that active powdered carbon to be ground into, by it
The water-bearing layer that is polluted by the oil is injected to repair pollution of underground aquifers caused by gas station's oil leak, takes full advantage of active powdered carbon to petroleum
The powerful adsorption capacity of hydrocarbon.Active carbon has powerful specific surface area and open structure, not only big to the adsorption capacity of petroleum hydrocarbon
Big enhancing, can also provide the place of survival and reproduction for degrading microorganism, increase microbial diversity and activity, be more advantageous to dirt
Contaminate the degradation of object.
2. two kinds of surfactants are added in renovation agent, they are adsorbed on the activated carbon, promote the dispersion of active powdered carbon
Stability after renovation agent is added to the water, has a small amount of surfactant release, the surfactant of these releases, energy
Enough promote the dissolution of diesel oil/gasoline in water, is more advantageous to degradation of the microorganism to them.
3. according to live injection experience many times, process scientifically strictly calculates, the distance between injector well is set as
3m, injection interlamellar spacing are set as 90cm, both ensure that cross one another between injection medicament in this way, seamless combination, and can guarantee injection
Well is not plugged up.
4. of the invention easily operated, used drug is environmentally protective, will not generate secondary pollution, medicament injection technique at
It is ripe, using convenient.It is combined by active carbon with microbial technique, activated carbon adsorption petroleum hydrocarbon latter aspect rapidly reduces water
The concentration of middle petroleum hydrocarbon, on the other hand, the high concentration petroleum hydrocarbon for being adsorbed on activated carbon surface can be attached on the surface again
Institute of Micro-biology's degradation makes active carbon continue to keep the adsorption capacity to petroleum hydrocarbon, in this way one absorption-degradation-of formation adsorb again-
The sustainable circulation removal mechanisms at work degraded again has relative to the absorption of simple activity charcoal or simple microbial degradation in water
Petroleum hydrocarbon to remove speed fast, and can thoroughly degrade, and adsorb and the reproducible advantage of degradation capability.
5. cost is relatively low for the technology, activated carbon adsorption combines compared with other technologies with microbial degradation, both economical real
With, and can be widely popularized.
Detailed description of the invention
Fig. 1 is adsorption isothermal curve of the renovation agent to phenol in water；
Fig. 2 is adsorption isothermal curve of the renovation agent to TCP in water；
Fig. 3 is that degradation bacteria, renovation agent and renovation agent-degradation bacteria combine degradation rate histogram to petroleum hydrocarbon in water；
Fig. 4 is position and the distribution map of North China Plain contaminated by diesel oil place injector well；
Fig. 5 is petroleum hydrocarbon concentration versus time curve figure in the underground water of North China Plain contaminated by diesel oil place；
Fig. 6 is position and the distribution map of Hebei gas station contaminated site injector well；
Fig. 7 is petroleum hydrocarbon concentration versus time curve figure in Hebei gas station contaminated site underground water；
Fig. 8 is position and the distribution map of Hebei refuse landfill contaminated site injector well；
Fig. 9 is toluene concentration versus time curve figure in Hebei refuse landfill contaminated site underground water.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Case study on implementation 1
The adsorption capacity of active powdered carbon is verified: renovation agent described in the inventive technique is used for the absorption of phenol in water.
A. selecting specific surface area is 1088.21m2/ g, the coconut activated powdered carbon that partial size is 1 μm；
B. by 0.2g Silane coupling agent KH550,0.33g neopelex, 0.26g Triton X-100,1g molecule
Amount is added in 1000ml deionized water for 9000 polysiloxanes, and A lapping liquid is made；
C. the Sodium Polyacrylate for being 40,000,000 by 2g relative molecular weight, the polysiloxanes that 1g molecular weight is 9000 are added 1L and go
In ionized water, B lapping liquid is made；
D. it after 1g active carbon particle and 0.5g A lapping liquid being mixed, is added in bidirectional rotation ball mill and grinds,
Grinding 48 hours, then 1g B lapping liquid is added into ball mill, then grind 48 hours.Active powdered carbon liquid after grinding is existed
It is centrifuged 1h under 8000r/min speed, outwells supernatant, is dried, renovation agent is made；
E. 30mg renovation agent is added in the phenol solution of 40ml various concentration, is 180r/min item in 20 DEG C, revolving speed
Then isothermal vibration 48h under part is centrifuged 30min under the conditions of 3000r/min.Take supernatant with it is ultraviolet-can be by light spectrophotometric
Concentration when meter measurement Phenol in Aqueous Solution balance.Adsorption isothermal curve is shown in attached drawing 1, as seen from the figure, reparation when experiment reaches balance
The equilibrium adsorption capacity of agent Pyrogentisinic Acid is 168.06mg/g.
Case study on implementation 2
Active powdered carbon adsorption capacity verifying: the renovation agent is used for the absorption of trichloropropane in water (TCP)
A. selecting specific surface area is 1088.21m2/ g, the coconut activated powdered carbon that partial size is 1 μm；
B. by 0.2g Silane coupling agent KH550,0.33g neopelex, 0.26g Triton X-100,1g molecule
Amount is added in 1000ml deionized water for 9000 polysiloxanes, and A lapping liquid is made；
C. the Sodium Polyacrylate for being 40,000,000 by 2g relative molecular weight, the polysiloxanes that 1g molecular weight is 9000 are added 1L and go
In ionized water, B lapping liquid is made；
D. it after 1g active carbon particle and 0.5g A lapping liquid being mixed, is added in bidirectional rotation ball mill and grinds,
Grinding 48 hours, then 1g B lapping liquid is added into ball mill, then grind 48 hours.Active powdered carbon liquid after grinding is existed
It is centrifuged 1h under the conditions of 8000r/min, outwells supernatant, dries, renovation agent is made.
E. 0.1g renovation agent is added in the TCP solution of 40ml various concentration, is 150r/min item in 25 DEG C, revolving speed
Then isothermal vibration 48h under part is centrifuged 30min under the conditions of 3000r/min；It takes in supernatant gas chromatograph for determination solution
Concentration when TCP is balanced.Adsorption isothermal curve is shown in attached drawing 2, it can be seen that balance of the renovation agent to TCP when reaching adsorption equilibrium
Adsorbance is 129.96mg/g.
Case study on implementation 3
A. minimal medium is prepared: by KH2PO41g、K2HPO41g、NH4NO31g、MgSO40.5g、
CaCl20.02g、FeSO4·7H2O 0.01g is added in 1L deionized water, with the NaOH solution of 0.1M by pH value
7.0 are adjusted to, it is spare after 121 DEG C of high pressure sterilization 30min are cooling.
B. oil-containing plating medium: diesel oil 1g is prepared, minimal medium 1L is spare through 121 DEG C of high pressure sterilization 30min,
Plating medium is made after taking 15ml oil-containing culture medium cooled and solidified.
C. the screening of efficient degrading bacteria: acquisition is coated on oil-containing plating medium by the oil-containing water sample of contaminated by diesel oil, dilution
On, 5 days are cultivated under the conditions of 30 DEG C up to growing bacterium colony, then carry out scribing line 4 times on fresh oil-containing plating medium again
It separated, purified, until growing single bacterium colony, strain after purification is tamed 3 times repeatedly on oil-containing culture medium flat plate, is obtained
Obtain efficient degradation strain.
Embodiment 4
By the petroleum hydrocarbon in active powdered carbon and degradation bacteria Joint adsorption degradation water.
A. efficient degrading bacteria obtained in renovation agent obtained in 1g embodiment 1 and 2ml embodiment 3 is added to 100ml
In oil-containing minimal medium, 30 DEG C on the oscillator are placed in 250ml conical flask, shake culture 15 under the conditions of 150r/min
It, while being compared with the oil-containing culture medium for only adding renovation agent and addition degradation bacteria, in addition not add any renovation agent
Blank control is done with the oil-containing culture medium of degradation bacteria.
B. after testing, 20ml normal hexane is added, is transferred to liquid-liquid extraction in separatory funnel, collects upper liquid after layering,
Subnatant again with n-hexane extraction twice, merge upper liquid drying anhydrous sodium sulfate filter, with UV-Vis spectrophotometry light
The light absorption value of degree meter measurement filtered fluid, calculates petroleum hydrocarbon concentration, to calculate the degradation rate of petroleum hydrocarbon.Experimental result is shown in attached drawing 3,
It is respectively 14.88%, 78.45% that degradation bacteria, renovation agent and renovation agent-degradation bacteria, which are combined to the degradation rate of petroleum hydrocarbon in water,
With 86.89%.
Degradation rate calculates:
Degradation rate=(C1-C2)/C1*100%
C1-blank control group Residual oil concentration (mg/L)；
C2-blank control group Residual oil concentration (mg/L)
Embodiment 5
A. by renovation agent made from 0.4t embodiment 1 and 1.2m3Efficient degradation bacterium solution made from embodiment 3 is added to 40m3
In part minimal medium, is stirred with impeller-type stirrer, be well-dispersed in renovation agent and microbial inoculum in system, closure is made
Liquid, the amount of this liquid for plugging are the average injection rate of individual well.
B. by 19kg renovation agent and 0.57m3Efficient degradation bacterium solution is added to 38m3In part minimal medium, vane type is used
Blender stirring, is well-dispersed in renovation agent in system, barrier liquid is made, and the amount of this barrier liquid is the average injection of individual well
Amount.
C. the verifying that the recovery technique effect is carried out in North China Plain contaminated by diesel oil place, is investigated 20m in the place
The above water-bearing layer is heavily contaminated, and total petroleum hydrocarbon concentration reaches 3.93mg/L in underground water.
D. the place highest level of ground water is 3m, and water-bearing layer distance from bottom earth's surface 20m, pollution sources are apart from earth's surface 1m.Along ground
It is lauched and flows to downstream direction and lay injector well within the scope of pollution sources 3m, attached drawing 4 is shown in the position of injector well and distribution, will block
Liquid establishes blotter coat using gunite injection underground.Distance is 3m between injector well.
E. blotter coat is established by gunite, uses the direct drilling machine with medicament ejector head in pollution sources along underground water first
It flows to and carries out directly pushing away drilling within the scope of downstream direction 3m.Injector head connection injection pressure is 3MPa, and flow is the mud of 60L/min
Pump, slush pump from capacity be 10m3Liquid for plugging holding vessel draw liquid for plugging.When injection injection, injector head is pressed onto underground first
The position 20m, well head overpressure is 15 atmospheric pressure when injection, and initial injection flow is maintained at 20L/s.The amount of injection is every time
2.0m3, after completing primary injection, injector head is mentioned to 90cm upwards and carries out second of injection again, until eject position and pollution sources
Terminate injection after maintaining an equal level.It completes after injecting for the first time, then carries out the injection of second mouthful of well.
F. IPRB is established by injection, uses the direct drilling machine with medicament ejector head in points of contamination along direction of groundwater flow first
It carries out directly pushing away drilling within the scope of 3 meters of downstream direction.Injector head connection injection pressure is 3MPa, the mud that flow is 30L/ minutes
Pump, slush pump from capacity be 10m3Holding vessel draw barrier liquid.When injection injection, injector head is pressed onto underground 20m first
It sets, well head overpressure is 15 atmospheric pressure when injection, and initial injection flow remains 20L/s.Each the amount of injection is 1.9m3。
After completing primary injection, injector head is mentioned to 90cm upwards and carries out second of injection again, until eject position is reached apart from earth's surface 2m.
It completes after injecting for the first time, then carries out the injection of second mouthful of well.
G. by after the completion of liquid for plugging and barrier liquid injection, taken at regular intervals water sample is carried out from inspection well and carries out petroleum hydrocarbon concentration
Analysis test, thus degradation effect of the analysis to total petroleum hydrocarbon in the place underground water.Petroleum hydrocarbon concentration is at any time in underground water
Variation see attached drawing 5, the concentration for injecting petroleum hydrocarbon in medicament the latter moon drops to 0.05mg/L by 3.93mg/L, continues monitoring 3
It rebounds never again in a month, illustrating to repair using underground water of the technology of the present invention to the place can reach good
Effect.
Embodiment 6
A. by 0.12t renovation agent and 0.36m3Efficient degrading bacteria is added to 12m3In part minimal medium, stirred with vane type
Device stirring is mixed, renovation agent and microbial inoculum is well-dispersed in system, liquid for plugging is made, the amount of this liquid for plugging is the average note of individual well
Enter amount.
B. 6.5kg renovation agent and 195L efficient degrading bacteria are added to 13m3In part minimal medium, stirred with vane type
Device stirring is mixed, renovation agent is well-dispersed in system, barrier liquid is made, the amount of this barrier liquid is the average injection rate of individual well.
C. in Hebei gas station, oil accident occurs for gasoline pipeline, using recovery technique described in the invention to pollution
Place underground water is repaired.Underground water is heavily contaminated, and total petroleum hydrocarbon concentration reaches 6.51mg/L in water.
D. the place level of ground water is 1m, and contaminant water water-bearing layer distance from bottom earth's surface 6m, pollution sources are apart from earth's surface 0.5m.
Injector well is laid within the scope of pollution sources 3m downstream along subsurface flow, attached drawing 6 is shown in the position of injector well and laying, will
Liquid for plugging establishes blotter coat using gunite injection underground.Distance is 3m between injector well.
E. blotter coat is established by gunite, uses the direct drilling machine with medicament ejector head in pollution sources along underground water first
It flows to and carries out directly pushing away drilling within the scope of downstream direction 5m.Injector head connection injection pressure is 5MPa, and flow is the mud of 80L/min
Pump, slush pump from capacity be 20m3Liquid for plugging holding vessel draw liquid for plugging.When injection injection, injector head is pressed onto underground first
The position 7m, well head overpressure is 15 atmospheric pressure when injection, and initial injection flow is maintained at 20L/s.The amount of injection is every time
2.0m3, after completing primary injection, injector head is mentioned to 90cm upwards and carries out second of injection again, until eject position and pollution sources
Terminate injection after maintaining an equal level.It completes after injecting for the first time, then carries out the injection of second mouthful of well.
F. IPRB is established by injection, uses the direct drilling machine with medicament ejector head in points of contamination along direction of groundwater flow first
It carries out directly pushing away drilling within the scope of 5 meters of downstream direction.Injector head connection injection pressure is 3MPa, the mud that flow is 30L/ minutes
Pump, slush pump from capacity be 20m3Holding vessel draw barrier liquid.When injection injection, injector head is pressed onto underground 7m first
It sets, well head overpressure is 15 atmospheric pressure when injection, and initial injection flow remains 20L/s.Each the amount of injection is 2.0m3。
After completing primary injection, injector head is mentioned to 90cm upwards and carries out second of injection again, until eject position is reached apart from earth's surface
0.5m.It completes after injecting for the first time, then carries out the injection of second mouthful of well.
G. by after the completion of liquid for plugging and barrier liquid injection, taken at regular intervals water sample is carried out from inspection well and carries out petroleum hydrocarbon concentration
Analysis test, thus degradation effect of the analysis to total petroleum hydrocarbon in the place underground water.Total petroleum hydrocarbon concentration is at any time in underground water
Between variation see attached drawing 7, petroleum hydrocarbon concentration is dropped within injection medicament the latter moon by 6.51mg/L in sampling of ground water as seen from the figure
To 0.02mg/L, continue to rebound never again in monitoring 5 months.
Embodiment 7
For the contaminated site of Hebei refuse landfill, there are the underground water pollution that toluene concentration is 120mg/L, pollutions
Source utilizes the invention skill for the contaminated site underground water for 3~8m apart from earth's surface 1.5m, level of ground water 3m, pollution depth
Art prepares liquid for plugging and barrier liquid, lays injector well and carries out medicament injection, it is dense that laying monitoring well carries out toluene in later period underground water
Degree monitoring, the infusion protocol of the distribution method and medicament of injector well and monitoring well are shown in attached drawing 8, individual well is average with case study on implementation 4
The amount for injecting medicament is 14m3Liquid for plugging and 13m3Obstruct liquid.Taken at regular intervals water sample, which is carried out, from inspection well after injection medicament carries out first
The analysis of benzene concentration is tested, thus degradation effect of the analysis to toluene in the place underground water.In underground water the concentration of toluene with
Attached drawing 9 is shown in the variation of time.After injection medicament in three months, the concentration of toluene falls below 1.2mg/L in underground water, and removal rate reaches
99%, effect is obvious.
Claims (10)
1. a kind of activity powdered carbon-degradation bacteria composition, it is characterised in that: active powdered carbon-degradation bacteria composition includes: efficient degradation
Strain, active powdered carbon, Silane coupling agent KH550, neopelex, Triton X-100, polysiloxanes, Sodium Polyacrylate
And deionized water.
2. a kind of activity powdered carbon-degradation bacteria composition groundwater remediation technology, it is characterised in that: the following steps are included:
A, Silane coupling agent KH550, neopelex, Triton X-100, polysiloxanes are add to deionized water system
At A lapping liquid, wherein the molecular weight of polysiloxanes is 800-1000；
B, by Sodium Polyacrylate, polysiloxanes is added in deionized water, and B lapping liquid is made；Wherein opposite point of Sodium Polyacrylate
Son amount is 40,000,000；
C, it after active powdered carbon and A lapping liquid being mixed, is added in bidirectional rotation ball mill and grinds, then add into ball mill
Enter B lapping liquid, then grind, then by the mixed liquor high speed centrifugation after grinding, outwell supernatant, dries, that is, renovation agent is made；
D, by efficient degradation strain in oil-containing culture medium amplification cultivation, by the efficient degradation strain and nothing after renovation agent, amplification
Machine salt culture medium is 1:2~5:100 mixing in mass ratio, is stirred with impeller-type stirrer, keeps renovation agent and strain equal in water
Even dispersion, is made liquid for plugging；
E, by after renovation agent, amplification efficient degradation strain and minimal medium be in mass ratio 1:20~50:2000 mix,
It is stirred with impeller-type stirrer, keeps renovation agent and strain evenly dispersed in water, barrier liquid is made；
F, leakage point establishes blotter coat: use first the direct drilling machine with medicament ejector head leakage point along subsurface flow downstream
It carrying out directly pushing away drilling within the scope of 2~5 meters of direction, injector head connects slush pump, and slush pump draws liquid for plugging from liquid for plugging holding vessel,
After completing primary injection, injector head is mentioned to 90cm upwards and carries out second of injection again, until eject position and oil leak point height are held
It is flat；
G, points of contamination establishes hydrodynamic seepage pressure (IPRB) in situ: using the direct drilling machine with medicament ejector head in points of contamination first
It carries out directly pushing away drilling along subsurface flow downstream 2~5 meters, injector head connection slush pump, slush pump is from liquid for plugging
Holding vessel draws liquid for plugging, after completing primary injection, injector head is mentioned to 90cm upwards and carries out second of injection again, until spraying position
Arrival is set apart from earth's surface 2m；It completes to inject for the first time, forms first injector well；
H, step f-g is repeated, the injection of second mouthful of injector well is carried out on 3 meters of remote positions of first injector well again.
3. a kind of active powdered carbon-degradation bacteria composition groundwater remediation technology according to claim 1, it is characterised in that:
The efficient degradation strain is efficient diesel or gasoline degradation strain, screening process are as follows: acquisition is by diesel oil or gasoline contamination
Oil-containing water sample, dilution, is coated on oil-containing plating medium and turns out bacterium colony, and scribing line separation, purifying, strain after purification is new
It is tamed 3-4 times repeatedly on fresh oil-containing culture medium flat plate, obtains efficient diesel or gasoline degradation strain.
4. a kind of active powdered carbon-degradation bacteria composition groundwater remediation technology according to claim 1, it is characterised in that:
The activity powdered carbon is one of cocoanut active charcoal, charcoal or bamboo charcoal, and the specific surface area of the activity powdered carbon is greater than 1000m2/
G, partial size are less than 0.5cm.
5. a kind of active powdered carbon-degradation bacteria composition groundwater remediation technology according to claim 2, it is characterised in that:
The component of the step d and the minimal medium in step f include: KH by ratio of weight and the number of copies2PO41 part, K2HPO4 1
Part, NH4NO31 part, MgSO40.5 part, CaCl20.02 part, FeSO47H20.01 part and 1000 parts of deionized water of O, and nothing
The pH of machine salt culture medium is 7.0；The minimal medium first passes around culture, and temperature is 30~40 DEG C, and incubation time is 3~
It is 5 days, then cooling after 121 DEG C, 30min high pressure sterilization again to use.
6. a kind of active powdered carbon-degradation bacteria composition groundwater remediation technology according to claim 2, it is characterised in that:
Oil-containing culture medium in the step a, step d and step f includes 1 part of diesel oil/gasoline and inorganic salts culture by ratio of weight and the number of copies
1000 parts of liquid, the plate oil-containing culture medium be oil-containing culture medium cooled and solidified after be made；The oil-containing culture medium first passes around
Culture, temperature are 30~40 DEG C, and incubation time is 3~5 days, then cooling after 121 DEG C, 30min high pressure sterilization again to use.
7. a kind of active powdered carbon-degradation bacteria composition groundwater remediation technology according to claim 2, it is characterised in that:
The mass ratio of active powdered carbon, A lapping liquid and B lapping liquid is 2:1:2 in the step c；The time ground again be 48~
72h, ultracentrifugal revolving speed are 8000~10000r/min, and centrifugation time is 30min or more.
8. a kind of active powdered carbon-degradation bacteria composition groundwater remediation technology according to claim 2, it is characterised in that:
Step d efficient degradation strain amplification cultivation in oil-containing culture medium, the strain number concentration after amplification are 108~1012A/
ml。
9. a kind of active powdered carbon-degradation bacteria composition groundwater remediation technology according to claim 2, it is characterised in that:
The injection pressure of slush pump is greater than 2.5MPa in the f step, and flow is greater than 50L/ minutes, and liquid for plugging storage tankage size is not less than
1m3；When injection injection, injector head will be pressed onto most deep projected depth, reach pollution water-bearing layer bottom as far as possible, or at least below underground
1 meter of water level or more；Depth is that the depth of oil leak is found when exploring early period when injection, and injection well head overpressure is not less than 15 atmosphere
Pressure, injection flow are initially held at 20L/s or more, and each the amount of injection is 1.5~2.0m3。
10. a kind of active powdered carbon-degradation bacteria composition groundwater remediation technology according to claim 2, it is characterised in that:
Slush pump injection pressure is greater than 2.5MPa in the g step, and flow is greater than 20L/ minutes, and barrier liquid storage tankage size is not less than
1m3；Injection injection when, first by injector head be pressed onto pollution water-bearing layer bottom, if directly push away drilling machine drilling depth cannot reach it is aqueous
Layer bottom, then do not use and directly push away drilling well injection mode, is changed to rotary drilling and constructs injection well injection；Well head overpressure when injection
Consistent with when establishing blotter coat with initial injection flow, each the amount of injection is 1.0m3More than.
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| CN114684887A (en) * | 2020-12-30 | 2022-07-01 | 中国石油化工股份有限公司 | In-situ remediation device and method for petroleum-type polluted underground water |
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