CN109042288A - A kind of perilla leaf new varieties mating system - Google Patents

A kind of perilla leaf new varieties mating system Download PDF

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Publication number
CN109042288A
CN109042288A CN201810779842.XA CN201810779842A CN109042288A CN 109042288 A CN109042288 A CN 109042288A CN 201810779842 A CN201810779842 A CN 201810779842A CN 109042288 A CN109042288 A CN 109042288A
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purple
perilla leaf
mating system
vegetation
leaf
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CN201810779842.XA
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李炳芹
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention relates to a kind of perilla leaf new varieties mating systems, perilla leaf self-mating system is cultivated including step (1) and step (2) breeding hybrid kind is bred, the perilla leaf planted by the above mating system compared with the existing technology in perilla leaf, its yield is significantly improved, in addition, perillaldehyde and purpurin have happy very big promotion, in addition, its anti-virus ability also has greatly improved, the advantages of the case where being not in variation, plantation that can be stable, the perilla leaf in the prior art that collected neat, blade area is big and thick, and performance is stablized.

Description

A kind of perilla leaf new varieties mating system
Technical field
The present invention relates to plant culture field, in particular to a kind of perilla leaf new varieties mating systems.
Background technique
Perilla leaf, Labiatae, Ta Hua race, Dicotyledoneae are annual, upright draft, south 7 to August, the north 8 To September.Stem is 0.3-2 meters high, green or purple, and blunt four prismatic has four slots, close villosity.The leaf band leaf of purple perilla and wild purple perilla Small switch, area of Chinese north and south each province is cultivated extensively.Bhutan, India, South East Asia Mainland, south is to Indonesia (Java), to the east of day This, Korea also has, and type specimen picks up from Japan, has good medicinal and edible value, the purple introduced from Japan to the country Perilla leaf variability is very big, and the second generation, the third generation all becomes grey substantially, cannot function as commodity plantation, so that its own can be lost Entrained effect, in addition, perilla leaf inferior quality in the prior art, the shape size and thickness of blade not phase Seemingly, some blade volumes having are big, but thickness is smaller, there are also fold, it is some be that vane thickness is big, but blade volume It is small, in addition, the component content of volatile oil and perillaldehyde is lower in perilla leaf, it is not well positioned to meet the demand of people, this hair It is bright to propose a kind of new mating system in view of the above problems.
Summary of the invention
The technical problem to be solved by the present invention is to perilla leaf mating system in the prior art is simple, is difficult to cultivate excellent Good perilla leaf kind, the perilla leaf performance cultivated is poor, and practicability is lower, the disease-resistant performance of perilla leaf during the growth process Also poor, it is unfavorable for large area plantation.
The technical scheme to solve the above technical problems is that a kind of perilla leaf new varieties mating system, including with Lower step:
(1) perilla leaf self-mating system is cultivated:
(1) six perilla leaf samples are chosen, then it is numbered, i.e., purple one, purple two, purple three, purple four, purple five and purple Six;
(2) the characteristics of six perilla leaf samples, is recorded:
Purple one, leaf are relatively thin greatly, and blade has fold, and plant is loose;
Purple two, leaf are thick, and blade is small, purple weight, and there are purple oil droplet, the short and adjacent perilla leaf of perilla leaf vegetation strain in top Vegetation growth comparatively dense;
Purple three, perilla leaf vegetation plant height, blade is big, and leaf is thick, but purple is shallower, and blade edge has green;
Purple four, perilla leaf vegetation growth are general, and cauline leaf is closer, and strain top lobus cardiacus has oil ring;
Purple five, the height of perilla leaf vegetation are slightly short in purple four, and blade purple weight, vane thickness is big and lobus cardiacus has oil ring;
Purple six, the plant height of perilla leaf vegetation, blade is big, and purple is general, and top leaf does not have oil ring.
(3) culture that six perilla leaf samples are carried out with 3 years by a definite date, to six perilla leaf samples when second year It is observed and is detected, eliminated the perilla leaf of purple and volatile oil difference, eliminate two perilla leaf samples in second year;In third year Also remaining purple two, purple three and purple five after eliminating one, 3 years again according to the variation size of remaining perilla leaf sample.
(4) force selfing: to remaining purple two after 3 years, purple three and purple five be selfed with fixing bag bagging method, with solid Determine bag timely bagging when starting to occur bud, then it is cultivated, will be fixed after purple two, purple three and purple are medium rare Bag and bud are cut together, and are stored to the seed in bud;
(5) then six perilla leaf samples are planted in control Tanaka by setting control field, while will be obtained in previous step Seed selects other one piece of experimental plot to be planted, and by control repeatedly in 3 years, then identifies it, finally eliminates three It is a, three are remained, i.e., purple two, purple three and purple five remain.
(2) breeding hybrid kind:
(1) Hybrid selects: three self-mating system relationships are close, but have biggish difference, and advantage and disadvantage are complementary, with purple two With purple three;Purple two and purple five;Purple three and purple five;It is carried out using the theory that three differences self-mating system level-one outstanding has complementary advantages miscellaneous It hands over, forms three pairs of combinations;
(2) hybridizing method:
1, the cultivation florescence is close, same environmental condition, same period sowing;
2, three pairs of self-mating system perilla leaf vegetation, purple two are hybridized with purple three, and purple two is hybridized with purple five, purple three and purple Five are hybridized;
3, vegetation bagging is hybridized to every a pair before there is bud with the biggish fixing bag of volume, by each pair of flower spike set Together, 8 points to 10 points progress artificial pollinations every morning operate continuously ten days to 12 days;
4, it is harvested after planting one end time, each pair of hybridization vegetation is connected into bag and is cut together, different positions is individually positioned in It sets, then the coming year three to April is sown into control field.
(3) parameter comparison: plantation is carried out according to the method described above for many years, and each year perilla leaf vegetation is subjected to parameter Comparison, quality relatively before perilla leaf sample increase, yield is higher than the 20%-30% of raw sample, and does not have The case where making a variation.
Based on the above technical solution, the present invention can also be improved as follows.
In any of the above-described scheme preferably, the fixing bag is template.
In any of the above-described scheme preferably, fixing bag described in (3) step in the step (1) having a size of Inside 15*5.
In any of the above-described scheme preferably, fixing bag ruler described in step 3 in (2) step in the step (2) Very little is inside 15*15.
In any of the above-described scheme preferably, the bagging time of fixing bag be September ten No. five to September 20 No. five it Between.
In any of the above-described scheme preferably, the time of cutting of fixing bag and bud is the time-division in the Frost's Descent.
In any of the above-described scheme preferably, the seed is stored by cloth bag.
In any of the above-described scheme preferably, the cloth bag prevents in the place that wind sheltering is protected from light.
In any of the above-described scheme preferably, pass through rope pair in third step in (2) step in the second step It is fixed in plant bar.
In any of the above-described scheme preferably, fixing bag and bud are sheared by scissors.
The beneficial effects of the present invention are: the perilla leaf that designed mating system is planted through the invention is relative to existing Perilla leaf in technology, yield are significantly improved, in addition, perillaldehyde and purpurin have happy very big promotion, in addition, its The case where anti-virus ability also has greatly improved, and is not in variation, plantation that can be stable, it is purple in the prior art to have collected neat The advantages of perilla leaf, blade area is big and thick, and performance is stablized.
Specific embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit Determine the scope of the present invention.
A kind of perilla leaf new varieties mating system, comprising the following steps:
(1) perilla leaf self-mating system is cultivated:
(1) six perilla leaf samples are chosen, then it is numbered, i.e., purple one, purple two, purple three, purple four, purple five and purple Six;
(2) the characteristics of six perilla leaf samples, is recorded:
Purple one, leaf are relatively thin greatly, and blade has fold, and plant is loose;
Purple two, leaf are thick, and blade is small, purple weight, and there are purple oil droplet, the short and adjacent perilla leaf of perilla leaf vegetation strain in top Vegetation growth comparatively dense;
Purple three, perilla leaf vegetation plant height, blade is big, and leaf is thick, but purple is shallower, and blade edge has green;
Purple four, perilla leaf vegetation growth are general, and cauline leaf is closer, and strain top lobus cardiacus has oil ring;
Purple five, the height of perilla leaf vegetation are slightly short in purple four, and blade purple weight, vane thickness is big and lobus cardiacus has oil ring;
Purple six, the plant height of perilla leaf vegetation, blade is big, and purple is general, and top leaf does not have oil ring.
(3) culture that six perilla leaf samples are carried out with 3 years by a definite date, to six perilla leaf samples when second year It is observed and is detected, eliminated the perilla leaf of purple and volatile oil difference, eliminate two perilla leaf samples in second year;In third year Also remaining purple two, purple three and purple five after eliminating one, 3 years again according to the variation size of remaining perilla leaf sample.
(4) force selfing: to remaining purple two after 3 years, purple three and purple five be selfed with fixing bag bagging method, with solid Determine pouch that bag the is done timely bagging when starting to occur bud, then it is cultivated;
(5) mid-October current year is together cut all bagging seeds connection pouch, and plant division is kept well, and next year three is below the moon Ten days sows six all self-mating systems with same type of seeding in different planting environments, that is, control field, by 3 years Control repeatedly, then it is identified, it is final to eliminate three, remain three, i.e., purple two, purple three and purple five retain Come.
(2) breeding hybrid kind:
(1) Hybrid selects: three self-mating system relationships are close, but have biggish difference, and advantage and disadvantage are complementary, with purple two With purple three;Purple two and purple five;Purple three and purple five;It is carried out using the theory that three differences self-mating system level-one outstanding has complementary advantages miscellaneous It hands over, forms three pairs of combinations;
(2) hybridizing method:
1, the cultivation florescence is close, same environmental condition, same period sowing;
2, three pairs of self-mating system perilla leaf vegetation, purple two are hybridized with purple three, and purple two is hybridized with purple five, purple three and purple Five are hybridized;
3, vegetation bagging is hybridized to every a pair before there is bud with the biggish fixing bag of volume, by each pair of flower spike set Together, 8 points to 10 points progress artificial pollinations every morning operate continuously ten days to 12 days;
4, it is harvested after planting one end time, each pair of hybridization vegetation is connected into bag and is cut together, different positions is individually positioned in It sets, then the coming year three to April is sown into control field.
(3) parameter comparison: plantation is carried out according to the method described above for many years, and each year perilla leaf vegetation is subjected to parameter Comparison, quality relatively before perilla leaf sample increase, yield is higher than the 20%-30% of raw sample, and does not have The case where making a variation.
In further specific embodiment, the fixing bag is template, and template is with good gas permeability and thoroughly Photosensitiveness can both prevent alienation from pollinating, and can also promote the growth of perilla leaf plant well.
In further specific embodiment, fixing bag described in (4) step in the step (1) is having a size of 15* 5 centimetres, it can be very good to be selfed purple two, purple three and purple five by fixing bag, prevent alienation from pollinating.
In further specific embodiment, fixing bag size described in step 3 in (2) step in the step (2) It is 15*15 centimetres, so as to hybridize well to the perilla leaf sample of selection.
In further specific embodiment, the bagging time of fixing bag be September ten No. five to September 20 No. five it Between.
In further specific embodiment, the time of cutting of fixing bag and bud is the time-division in the Frost's Descent.
In further specific embodiment, the seed is stored by cloth bag, and the gas permeability of cloth bag is preferable, can be with Seed is protected well.
In further specific embodiment, the cloth bag is prevented in the place that wind sheltering is protected from light.
In further specific embodiment, in the second step in (2) step in third step by rope for Plant bar is fixed.
In further specific embodiment, fixing bag and bud are sheared by scissors.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", " length ", " width ", " thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " up time The orientation or positional relationship of the instructions such as needle ", " counterclockwise ", " axial direction ", " radial direction ", " circumferential direction " be shown in orientation or position close System, is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must have Specific orientation is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.
In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or Implicitly include at least one this feature.In the description of the present invention, the meaning of " plurality " is at least two, such as two, three It is a etc., unless otherwise specifically defined.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc. Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral;It can be mechanical connect It connects, is also possible to be electrically connected;It can be directly connected, can also can be in two elements indirectly connected through an intermediary The interaction relationship of the connection in portion or two elements, unless otherwise restricted clearly.For those of ordinary skill in the art For, the specific meanings of the above terms in the present invention can be understood according to specific conditions.
In the present invention unless specifically defined or limited otherwise, fisrt feature in the second feature " on " or " down " can be with It is that the first and second features directly contact or the first and second features pass through intermediary mediate contact.Moreover, fisrt feature exists Second feature " on ", " top " and " above " but fisrt feature be directly above or diagonally above the second feature, or be merely representative of First feature horizontal height is higher than second feature.Fisrt feature can be under the second feature " below ", " below " and " below " One feature is directly under or diagonally below the second feature, or is merely representative of first feature horizontal height less than second feature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office It can be combined in any suitable manner in one or more embodiment or examples.In addition, without conflicting with each other, the skill of this field Art personnel can tie the feature of different embodiments or examples described in this specification and different embodiments or examples It closes and combines.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned Embodiment is changed, modifies, replacement and variant.

Claims (10)

1. a kind of perilla leaf new varieties mating system, which comprises the following steps:
(1) perilla leaf self-mating system is cultivated:
(1) six perilla leaf samples are chosen, then it is numbered, is i.e. purple one, purple two, purple three, purple four, purple five and purple six;
(2) the characteristics of six perilla leaf samples, is recorded:
Purple one, leaf are relatively thin greatly, and blade has fold, and plant is loose;
Purple two, leaf are thick, and blade is small, purple weight, and there are purple oil droplet, the short and adjacent perilla leaf vegetation of perilla leaf vegetation strain in top Grow comparatively dense;
Purple three, perilla leaf vegetation plant height, blade is big, and leaf is thick, but purple is shallower, and blade edge has green;
Purple four, perilla leaf vegetation growth are general, and cauline leaf is closer, and strain top lobus cardiacus has oil ring;
Purple five, the height of perilla leaf vegetation are slightly short in purple four, and blade purple weight, vane thickness is big and lobus cardiacus has oil ring;
Purple six, the plant height of perilla leaf vegetation, blade is big, and purple is general, and top leaf does not have oil ring.
(3) culture that six perilla leaf samples are carried out with 3 years by a definite date carries out six perilla leaf samples when second year Observation and detection, eliminate the perilla leaf of purple and volatile oil difference, eliminate two perilla leaf samples in second year;Third year according to The variation size of remaining perilla leaf sample also remaining purple two, purple three and purple five after eliminating one, 3 years again.
(4) force selfing: to remaining purple two after 3 years, purple three and purple five be selfed with fixing bag bagging method, use fixing bag The pouch done timely bagging when starting to occur bud, then cultivates it;
(5) mid-October current year is together cut all bagging seeds connection pouch, and plant division is kept well, and next year exists late March Six all self-mating systems are sowed with same type of seeding in different planting environments, that is, control field, it is anti-by 3 years Multiple control, then identifies it, final to eliminate three, remains three, i.e., purple two, purple three and purple five remain.
(2) breeding hybrid kind:
(1) Hybrid selects: three self-mating system relationships are close, but have biggish difference, and advantage and disadvantage are complementary, with purple two and purple Three;Purple two and purple five;Purple three and purple five;Hybridized using the theory that three differences self-mating system level-one outstanding has complementary advantages, Form three pairs of combinations;
(2) hybridizing method:
1, the cultivation florescence is close, same environmental condition, same period sowing;
2, three pairs of self-mating system perilla leaf vegetation, purple two three are hybridized with purple, and purple two is hybridized with purple five, purple three and purple five into Row hybridization;
3, vegetation bagging is hybridized to every a pair before there is bud with the biggish fixing bag of volume, each pair of flower spike is covered one It rises, 8 points to 10 points progress artificial pollinations every morning, operates continuously ten days to 12 days;
4, it is harvested after planting one end time, each pair of hybridization vegetation is connected into bag and is cut together, is individually positioned in different positions, so It is sown into control field the coming year three to April afterwards.
(3) parameter comparison: plantation is carried out according to the method described above for many years, and each year perilla leaf vegetation is subjected to parameter pair Than, quality relatively before perilla leaf sample increase, yield is higher than the 20%-30% of raw sample, and without going out The case where now making a variation.
2. perilla leaf new varieties mating system according to claim 1, which is characterized in that the fixing bag is template.
3. perilla leaf new varieties mating system according to claim 1, which is characterized in that in the step (1) (3) fixing bag described in step is having a size of 15*5 centimetres.
4. perilla leaf new varieties mating system according to claim 1, which is characterized in that (2) in the step (2) Fixing bag described in step 3 is having a size of 15*15 centimetres in step.
5. perilla leaf new varieties mating system according to claim 1, which is characterized in that the bagging time of the fixing bag Between September ten No. five to September 20 No. five.
6. perilla leaf new varieties mating system according to claim 1, which is characterized in that the fixing bag and bud Cutting the time is the time-division in the Frost's Descent.
7. perilla leaf new varieties mating system according to claim 1, which is characterized in that the seed passes through cloth bag It is stored.
8. perilla leaf new varieties mating system according to claim 1, which is characterized in that the cloth bag prevents from keeping away The place that wind is protected from light.
9. perilla leaf new varieties mating system according to claim 1, which is characterized in that described in the second step (2) plant bar being fixed by rope in third step in step.
10. perilla leaf new varieties mating system according to claim 1, which is characterized in that by scissors to fixing bag and Bud is sheared.
CN201810779842.XA 2018-07-16 2018-07-16 A kind of perilla leaf new varieties mating system Pending CN109042288A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111972280A (en) * 2020-08-24 2020-11-24 河北农业大学 Pollination method for improving purple perilla hybridization success rate
CN112535101A (en) * 2020-12-02 2021-03-23 太极集团重庆涪陵制药厂有限公司 Perilla frutescens fine variety breeding method

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CN103109740A (en) * 2013-03-08 2013-05-22 中国科学院近代物理研究所 New strain breeding method of high-quality purple perilla
CN107173215A (en) * 2017-06-08 2017-09-19 贵港市光速达电子科技有限公司 A kind of breeding method of purple perilla new varieties

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US7105719B1 (en) * 1995-02-17 2006-09-12 Suntory Limited Gene encoding a protein having acyl group transfer activity
CN103109740A (en) * 2013-03-08 2013-05-22 中国科学院近代物理研究所 New strain breeding method of high-quality purple perilla
CN107173215A (en) * 2017-06-08 2017-09-19 贵港市光速达电子科技有限公司 A kind of breeding method of purple perilla new varieties

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111972280A (en) * 2020-08-24 2020-11-24 河北农业大学 Pollination method for improving purple perilla hybridization success rate
CN112535101A (en) * 2020-12-02 2021-03-23 太极集团重庆涪陵制药厂有限公司 Perilla frutescens fine variety breeding method
CN112535101B (en) * 2020-12-02 2022-05-31 太极集团重庆涪陵制药厂有限公司 Perilla frutescens fine variety breeding method

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Application publication date: 20181221