CN109030666B - Method for identifying honeysuckle and lonicera confusa by utilizing high performance liquid chromatography - Google Patents
Method for identifying honeysuckle and lonicera confusa by utilizing high performance liquid chromatography Download PDFInfo
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Abstract
The invention discloses a method for identifying honeysuckle and lonicera confusa by utilizing high performance liquid chromatography, which is characterized in that when an object to be detected is one or two of the honeysuckle or the lonicera confusa, the honeysuckle and the lonicera confusa are identified by detecting the weight percentage of the nux vomica acid of a dried object to be detected by utilizing the high performance liquid chromatography, and when the content of the nux vomica acid of the dried object to be detected is less than 1.5%, the honeysuckle and the lonicera confusa are judged to be non-honeysuckle; the content of strychnic acid is more than 1.0%, and the product is determined to be flos Lonicerae. When the content of strychnic acid is less than or equal to 1.5% and more than or equal to 1.0%, the honeysuckle or the lonicera confusa is judged to be a mixture. The method for identifying the honeysuckle and the lonicera confusa by detecting the weight percentage of the strychnic acid in the honeysuckle and lonicera confusa dry product through the high performance liquid chromatography is quick and accurate, and provides a basis for identifying true and false in the production, circulation and use processes of the medicine.
Description
Technical Field
The invention belongs to the technical field of identification of source varieties of traditional Chinese medicinal materials, relates to a method for identifying honeysuckle and lonicera confusa, and particularly relates to a method for identifying honeysuckle and lonicera confusa by utilizing high performance liquid chromatography.
Background
Honeysuckle and lonicera confusa are listed as two medicinal materials from the pharmacopoeia of the people's republic of China 2005 edition. Because the species of the original plants of the lonicera confusa and the lonicera confusa are similar, the appearance and the shape of the medicinal materials are similar and difficult to distinguish, the honeysuckle and the lonicera confusa in the market are unclear in source and disordered in variety, because the lonicera confusa honeysuckle phenomenon frequently exists in the market due to large yield and low price, but the substance basis of the lonicera confusa honeysuckle is greatly different, so that the curative effect of the medicinal materials and the preparation thereof is directly influenced, more seriously, the lonicera confusa has more saponin content than the honeysuckle, and the saponin can cause the hemolysis of the injection, and the life safety of a user is directly harmed.
The honeysuckle and the lonicera confusa can be separated from other medicinal materials by using a traditional method, but the existing methods for identifying the varieties of the honeysuckle and the lonicera confusa have certain defects. For example, the traditional appearance and shape identification depends on experience and has large subjectivity, and particularly, the mixed product or the crushing state of the two products in the market cannot be identified; the DNA molecular identification method has complex technology, ten thousand-level experiment environment, high requirement, high cost, long inspection period and poor repeatability caused by a plurality of influencing factors. Pharmacological methods have high verification cost, long cycle and large errors caused by a plurality of influence factors, and although the methods can distinguish closely related varieties to a certain extent, the methods cannot evaluate the quality of the drug effect.
Patent CN 103173532B (a method for identifying honeysuckle and lonicera confusa and application thereof) relates to a method for identifying honeysuckle and lonicera confusa and application thereof. The method comprises PCR amplification of ITS2 nucleotide sequence, including 1) extraction of sample DNA; 2) PCR amplifying a fragment containing the ITS2 sequence of ribosomal DNA; 3) splicing the amplified products to obtain a complete ITS2 gene spacer region; 4) an NJ tree of ITS2 sequences was constructed for the samples. Patent CN 104419754 a (a method for rapidly identifying the authenticity of honeysuckle) discloses a method for identifying honeysuckle and common counterfeit lonicera confusa thereof, which is to analyze the marker nucleic acid sites of honeysuckle and lonicera confusa by using a high resolution dissolution curve technique. The method confirms the type of the marker nucleic acid locus by carrying out DNA extraction, PCR amplification and HRM analysis on a sample of the honeysuckle to be tested, and further identifies the authenticity of the honeysuckle. The method belongs to a DNA molecule identification method, requires PCR amplification, and has complex technology and high cost.
Patent CN 105277721B (a method for identifying lonicera confusa and lonicera confusa raw materials of a preparation for treating stomatitis) discloses an identification method for distinguishing lonicera confusa and lonicera confusa, which comprises the following steps: taking flos Lonicerae or preparation containing flos Lonicerae as control; constructing an acute oral inflammation model; the influence of the test sample and the reference standard on the expression levels of inflammatory factors TNF-alpha, IL-6, IL-8 and IL-10 in the acute oral inflammation model cells is compared, and the honeysuckle and the lonicera confusa or the compound preparation thereof are distinguished. The method requires lonicera confusa or a preparation containing lonicera confusa raw materials as a control, and an acute oral inflammation model is constructed; the method has the defects of complex detection method, insufficient and rapid detection, high cost, a plurality of influencing factors and large errors.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a method for identifying honeysuckle and lonicera confusa by using high performance liquid chromatography. The method takes the strychnic acid as an index, and adopts liquid chromatography to detect the weight percentage of the strychnos acid in the honeysuckle and lonicera confusa dry products so as to identify the honeysuckle and the lonicera confusa.
In order to achieve the purpose, the invention adopts the following technical scheme:
the method for identifying the honeysuckle and the lonicera confusa by utilizing the high performance liquid chromatography detects the weight percentage of the strychnic acid in the honeysuckle and lonicera confusa dry product by utilizing the high performance liquid chromatography to identify the honeysuckle and the lonicera confusa when the object to be detected is one or two of the honeysuckle and the lonicera confusa.
Preferably, in the above identification method, when the content of strychnic acid is less than 1.5%, the honeysuckle is judged to be non-honeysuckle; judging whether the content of strychnic acid is more than 1.0 percent or not; when the content of strychnic acid is less than or equal to 1.5% and more than or equal to 1.0%, the honeysuckle or the lonicera confusa is judged to be the mixture.
Preferably, the detection method for identifying the content of strychnic acid in honeysuckle and lonicera confusa comprises the following steps: and (3) injecting the reference substance solution and the test solution into a high performance liquid chromatograph, measuring peak areas at the wavelength of 200-280nm, and calculating by adopting an external standard method or a standard curve method to obtain the contents of the interruptive strychnic acid in the honeysuckle and the lonicera confusa.
In the above method, the sample volume of the reference solution and the sample solution is 2-25 μ L.
In one step, the sample amount of the reference solution and the sample solution is preferably 2-20 μ L.
More preferably, the wavelength range of the detection method is 230 to 254 nm.
Further preferably, the optimal wavelength of the detection method is 240 nm.
Preferably, the measurement conditions of the high performance liquid chromatography are as follows:
column C18, 4.6mm X250 mm, 5 μm; the wavelength is 200-280 nm; using acetonitrile as a mobile phase A, using an acid water solution with the pH value of 1.5-4 as a mobile phase B, and adopting gradient elution, wherein the mobile phase A is 0-20 min, and the volume percentage is 5% → 15%; 20-25 min, 15% → 23% by volume; the balance is mobile phase B.
Further preferably, the acid aqueous solution comprises one or more of phosphoric acid, formic acid, acetic acid and phosphate.
Further preferably, the preparation step of the control solution comprises: precisely weighing semen Strychni Pulveratum acid reference, and adding solvent to obtain 0.05-0.5mg/mL solution.
Preparation of a test solution: taking 0.25g of test sample powder, precisely weighing, placing in a conical flask with a plug, precisely adding 10-50mL of solvent, carrying out ultrasonic treatment for 15-60 minutes, shaking up, and filtering to obtain the product.
Further preferably, the power of the ultrasound is 250W, and the frequency is 35 kHz.
Further preferably, the solvent is one or more of water, 0-50% ethanol solution or 0-50% methanol solution.
The invention has the beneficial effects that:
the method for identifying the honeysuckle and the lonicera confusa by detecting the weight percentage of the strychnic acid in the honeysuckle and lonicera confusa dry product through the high performance liquid chromatography is quick and accurate, and provides a basis for identifying true and false in the production, circulation and use processes of the medicine.
Drawings
FIG. 1 is a spectrum of a strychnic acid reference substance;
FIG. 2 is a spectrum of honeysuckle;
FIG. 3 is a flos Lonicerae map;
FIG. 4 shows the spectrogram of flos Lonicerae of different producing areas;
FIG. 5 is a chart of flos Lonicerae with different origins, different solvents and different drying modes;
FIG. 6 is a spectrum of Lonicera confusa at a detection wavelength of 254 nm;
FIG. 7 is a spectrum of different batches of flos Lonicerae from Henan;
FIG. 8 is a spectrum of different batches of flos Lonicerae from Hebei;
FIG. 9 is a spectrum of different batches of flos Lonicerae of Shandong.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and examples, which are provided for the purpose of illustration only and are not intended to limit the scope of the invention.
Example 1 establishing a detection method
1. Apparatus and materials
1.1 instruments
Agilent 1260 binary high performance liquid chromatograph, Agilent G4212B diode array detector, Agilent Eclipse XDB-C18Chromatography column (150mm × 4.6mm, 5 μm), KH500B ultrasonic cleaner (Kunshan GRAD), and water system of color development D24UV ultrapure water (Michibo China).
1.2 materials and reagents
Acetonitrile (Fisher scientific) is in chromatographic grade, water is in first-grade ultrapure water, and other reagents such as absolute ethyl alcohol (COMIO) are in analytical grade; the seconux vomica acid reference substance is purchased from Shanghai Shidande Standard technical service Co., Ltd, and has the following batch numbers: 60077-46-5.
2. Test method
2.1 chromatographic conditions
Column C18, 4.6mm X250 mm, 5 μm; the wavelength is 240 nm; taking acetonitrile as a mobile phase A, taking a phosphoric acid aqueous solution with the pH value of 2.0 as a mobile phase B, and adopting gradient elution, wherein the mobile phase A is 0-20 min, and the volume percentage is 5% → 15%; 20-25 min, 15% → 23% by volume; the balance is mobile phase B.
2.2 preparation of control solutions
Precisely weighing semen Strychni Pulveratum acid reference, and adding 10% ethanol to obtain 0.27mg/mL solution.
2.3 preparation of test solutions
Taking about 0.25g of sample powder (passing through a No. four sieve), precisely weighing, placing in a conical flask with a plug, precisely adding 25ml of 10% ethanol, performing ultrasonic treatment (power 250W and frequency 35kHz) for 30 minutes, shaking up, and filtering to obtain the product.
2.4 methodological Studies
2.4.1 Linear investigation
2, 5, 10, 15, 20 and 25. mu.l of control solutions (0.27mg/mL) were precisely aspirated and injected into a liquid chromatograph, respectively, and peak areas were recorded. Linear regression was performed on the sample injection mass (m, μ g) with peak area (a), the regression equation being: a is 1182C-24.2, and the correlation coefficient R is 0.9998, so that the strychnine-breaking acid solution has a good linear relationship between 0.54 and 6.75 mu g. The results are shown in Table 1.
TABLE 1 Linear relationship examination
2.4.2 precision of the Instrument
A10. mu.L of control solution (0.27mg/mL) was precisely aspirated, injected into a liquid chromatograph, and the peak area was recorded, and the precision of the apparatus was calculated as follows:
TABLE 2 Instrument precision investigation
The results show that: the mean peak area 3182.2, RSD 0.43%, instrument precision was good.
2.4.3 repeatability
Taking the same batch of samples (flos Lonicerae produced in Shandong), taking 6 parts in parallel, preparing the test solution and the reference solution according to the content determination method, precisely sucking 10 μ L of each solution, injecting into a liquid chromatograph, recording peak area, and calculating content. The results are as follows:
TABLE 3 repeatability test investigation
The results show that: RSD 1.83%, the repeatability of the experiment was good.
2.5 sample determination
And (3) taking 10 mu L of each of the reference solution and the test solution, injecting into a high performance liquid chromatograph, measuring the peak area at 240nm, and calculating by adopting an external standard method to obtain the content of the strychnic acid in the honeysuckle and the lonicera confusa. The results are shown in FIGS. 1-3.
Example 2 verification experiment
1. And (5) verifying the honeysuckle in different producing areas.
The content of the honeysuckle in different producing areas is determined by the detection conditions of the method in the example 1, and the result is shown in figure 4.
The spectrogram in the figure sequentially provides Ganhua in Chongqing Jiefang Lonshan, Ganhua in Shandong Baoli in Guizhou, Ganhua in Shandong Baoli and Ganhua in Hunan from top to bottom.
The control spectrum of the combination of the seconux vomica acid and the graph of FIG. 4 can be seen: the corresponding position of the strychnic acid reference substance is not provided with or has trace chromatographic peaks in different lonicera confusa maps, and the calculated content is far lower than 1.0 percent.
2. And (4) verifying the lonicera confusa under the conditions of different producing areas, different solvents, different drying modes and the like.
The honeysuckle in different producing areas is taken as a research object, different solvents are adopted for extraction, the content is measured according to the detection conditions of the method in the embodiment 1, and the spectrogram is shown in figure 5.
The spectra shown in fig. 5 are, from top to bottom: lonicera hypoglauca Miq 1 (solvent is water), a reference substance (solvent is water), Lonicera fulvescens (solvent is 50% methanol), Lonicera virescens (solvent is 50% methanol), Lonicera sempervirens (solvent is 50% methanol), Lonicera 3 (solvent is 50% methanol), Lonicera 2 (solvent is 50% methanol), Lonicera nanninghamiana (solvent is water), Lonicera 3 (solvent is water), Lonicera 4 (solvent is water), Lonicera 5 (solvent is water), Lonicera feltiana (solvent is water), Lonicera fulvescens (solvent is water), Lonicera anhui Hongmansiana (solvent is water), Lonicera freeze-dried Lonicera (solvent is water), Lonicera hainanensis (solvent is water), and Lonicera honggmansiana camara (solvent is water).
As can be seen from fig. 5: water and 50% methanol can be used as extraction solvent of semen Strychni Pulveratum acid; the lonicera confusa spectra extracted from different producing areas and different solvents have no or trace chromatographic peak at the corresponding position of the strychnic acid reference substance, and the content of the strychnic acid is far lower than 1.0 percent by calculation.
3. Sample validation at different wavelengths
The content of different batches of the grey felt hairy honeysuckle flowers is measured at 254nm according to the detection conditions of the method described in example 1, and the result is shown in figure 6.
The spectrogram in fig. 6 is as follows from top to bottom: the composition comprises a strychnos alkaloid reference substance, a crinis ustus, a lonicera macranthoides 1 st batch, a lonicera macranthoides 2 nd batch, a lonicera macranthoides 3 rd batch, a lonicera macranthoides 4 th batch, a lonicera macranthoides 5 th batch and a lonicera macranthoides 6 th batch.
As can be seen from fig. 6: 254nm can be used as the detection wavelength for detecting the strychnine-split acid; the corresponding position of the disrupted strychnic acid reference substance of different batches of lonicera confusa spectrums has no or trace chromatographic peak, and the calculated content is far lower than 1.0 percent.
4. Verification of honeysuckle samples of different batches, different market specifications and different production places
Different batches of honeysuckle with different market specifications and different producing areas (Shandong, Henan) are taken as research objects, the content is determined according to the detection conditions of the method in the embodiment 1, and the results are shown in figures 7-9.
The spectrogram in fig. 7 is as follows from top to bottom: the first 1 st, the second 2 nd, the 3 rd, the 4 th and the 5 th of the second.
The spectrogram in fig. 8 is as follows from top to bottom: the 3 rd batch of the three-grade product of Hebei, the 1 st batch of the three-grade product of Hebei and the 2 nd batch of the three-grade product of Hebei.
The spectrogram in fig. 9 is as follows from top to bottom: batch 4, batch 1, batch 2 and batch 3.
The control pattern of the combination of mucic acid and FIGS. 7-9 can be seen: the corresponding position of the strychnic acid reference substance has obvious chromatographic peaks in different batches, different market specifications and different producing areas of the honeysuckle flower map, and the calculated content is far higher than 1.5 percent.
5. Semen Strychni acid content of flos Lonicerae of different producing areas and different batches
Each batch of honeysuckle in the north Hebei province, the south Henan province and the Shandong province is detected according to the detection conditions of the method in the example 1, and the detection results are shown in table 4.
TABLE 4 detection results of Lonicera japonica Thunb in Hebei province, Henan province and Shandong province
6. The content of strychnos acid in flos Lonicerae of different producing areas and different batches is interrupted
Various batches of lonicera confusa in Guangdong province, Guizhou province and Hunan province are respectively detected according to the detection conditions of the method in the example 1, and the detection results are shown in Table 5.
TABLE 5 detection results of flos Lonicerae of Guangdong province, Guizhou province and Hunan province
The honeysuckle and the lonicera confusa can be quickly and accurately identified by detecting the honeysuckle and the lonicera confusa in different producing areas and different batches.
Although the embodiments of the present invention have been described with reference to the accompanying drawings, the scope of the present invention is not limited thereto, and various modifications and variations which do not require inventive efforts and which are made by those skilled in the art are within the scope of the present invention.
Claims (3)
1. The method for identifying the honeysuckle and the lonicera confusa by utilizing the high performance liquid chromatography is characterized in that when the dry product to be detected is one or two of the honeysuckle or the lonicera confusa, the weight percentage of the strychnic acid of the dry product to be detected is detected by the high performance liquid chromatography to identify the honeysuckle and the lonicera confusa; the method comprises the following specific steps:
(1) preparation of a test solution: taking 0.25g of test sample powder, precisely weighing, placing in a conical flask with a plug, precisely adding 10-50mL of solvent, performing ultrasonic treatment for 15-60 minutes, shaking up, and filtering to obtain the product;
(2) preparation of control solutions: precisely weighing semen Strychni Pulveratum acid reference, and adding solvent to obtain 0.05-0.5mg/mL solution;
in the step (1) and the step (2), the solvent is one or more of water, 0-50% ethanol solution or 0-50% methanol solution;
(3) the detection method of the weight percentage of the strychnos nux-vomica acid comprises the following steps: injecting the reference solution and the test solution into a high performance liquid chromatograph, measuring peak areas at the wavelength of 200-280nm, and calculating by adopting an external standard method or a standard curve method to obtain the contents of the interrupting strychnic acid in the honeysuckle and the lonicera confusa;
the determination conditions of the high performance liquid chromatography are as follows: column C18, 4.6mm X250 mm, 5 μm; the wavelength is 200-280 nm; acetonitrile is used as a mobile phase A, a phosphoric acid aqueous solution with the pH value of 1.5-4 is used as a mobile phase B, and gradient elution is adopted, wherein the mobile phase A is 0-20 min, and the volume percentage is 5% → 15%; 20-25 min, 15% → 23% by volume; the rest is mobile phase B;
judging the dried product to be tested to be non-honeysuckle if the weight percentage of the semen strychnic acid is less than 1.5%; if the weight percentage of the strychnic acid is more than 1.0 percent, the honeysuckle flower is judged to be non-mountain honeysuckle flower.
2. The method for identifying honeysuckle and lonicera confusa as claimed in claim 1, wherein the detection wavelength is 230-254 nm.
3. The method as claimed in claim 1, wherein the power of the ultrasonic wave is 250W and the frequency is 35 kHz.
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| CN111198236A (en) * | 2020-01-14 | 2020-05-26 | 山东中医药大学 | Method for identifying honeysuckle and lonicera confusa by utilizing high performance liquid chromatography and liquid chromatography-mass spectrometry |
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| CN114689753A (en) * | 2022-03-31 | 2022-07-01 | 广东一方制药有限公司 | Method for identifying honeysuckle in different Yuanyuan mountain |
| CN115792070A (en) * | 2022-10-28 | 2023-03-14 | 真奥金银花药业有限公司 | Method for measuring content of iridoid component in honeysuckle oral liquid |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100359251B1 (en) * | 1994-12-28 | 2003-01-08 | 주식회사 엘지생명과학 | Method for extracting 5-hydroxymethyl-2-furfural from lonicera japonica thunb |
| CN101628023A (en) * | 2009-02-24 | 2010-01-20 | 广州白云山和记黄埔中药有限公司 | Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof |
| CN103808751A (en) * | 2013-01-30 | 2014-05-21 | 河南省科高植物天然产物开发工程技术有限公司 | Method for identifying lonicera japonica medicinal material or derivatives of lonicera japonica medicinal material |
| CN105606734A (en) * | 2016-01-05 | 2016-05-25 | 华中科技大学 | Method for detecting honeysuckle flower and lonicerae flos medicinal materials through rapid resolution liquid chromatography |
| CN106226415A (en) * | 2016-07-11 | 2016-12-14 | 山东省分析测试中心 | A kind of method of six kinds of iridoid glycoside constituents in Flos Lonicerae of mensuration simultaneously |
| CN106248817A (en) * | 2016-07-11 | 2016-12-21 | 山东省分析测试中心 | A kind of quality of Flos Lonicerae evaluation methodology |
| CN108469473A (en) * | 2018-02-09 | 2018-08-31 | 山东省分析测试中心 | A method of utilizing metabolite of the RRLC-DAD-ESI-Q-TOF-MS detection honeysuckles in rat blood serum |
-
2018
- 2018-10-09 CN CN201811173053.8A patent/CN109030666B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100359251B1 (en) * | 1994-12-28 | 2003-01-08 | 주식회사 엘지생명과학 | Method for extracting 5-hydroxymethyl-2-furfural from lonicera japonica thunb |
| CN101628023A (en) * | 2009-02-24 | 2010-01-20 | 广州白云山和记黄埔中药有限公司 | Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof |
| CN103808751A (en) * | 2013-01-30 | 2014-05-21 | 河南省科高植物天然产物开发工程技术有限公司 | Method for identifying lonicera japonica medicinal material or derivatives of lonicera japonica medicinal material |
| CN105606734A (en) * | 2016-01-05 | 2016-05-25 | 华中科技大学 | Method for detecting honeysuckle flower and lonicerae flos medicinal materials through rapid resolution liquid chromatography |
| CN106226415A (en) * | 2016-07-11 | 2016-12-14 | 山东省分析测试中心 | A kind of method of six kinds of iridoid glycoside constituents in Flos Lonicerae of mensuration simultaneously |
| CN106248817A (en) * | 2016-07-11 | 2016-12-21 | 山东省分析测试中心 | A kind of quality of Flos Lonicerae evaluation methodology |
| CN108469473A (en) * | 2018-02-09 | 2018-08-31 | 山东省分析测试中心 | A method of utilizing metabolite of the RRLC-DAD-ESI-Q-TOF-MS detection honeysuckles in rat blood serum |
Non-Patent Citations (6)
| Title |
|---|
| HPLC-DAD-ESI-Q-TOF/MS法测定金银忍冬花中的化学成分;朱姮 等;《中草药》;20170630;第48卷(第11期);第2300-2305页 * |
| Influence of Sulfur Fumigation on the Chemical Constituents and Antioxidant Activity of Buds of Lonicera japonica;Ai-Li Guo 等;《Molecules》;20141015;第19卷;第16640-16655页 * |
| Secoiridoid Sulfonates from the Sulfiting-Processed Buds of Lonicera japonica;Zong‐Yun Li 等;《Helvetica Chimica Acta》;20120711;第95卷;第1144-1151页 * |
| Structural characterization and identification of iridoid glycosides, saponins, phenolic acids and flavonoids in Flos Lonicerae Japonicae by a fast liquid chromatography method with diode-array detection and time-of-flight mass spectrometry;Lian‐Wen Qi 等;《Rapid Communications in Mass Spectrometry》;20090901;第23卷;第3227-3242页 * |
| 基于UFLC-Triple-Q-TOF-MS/MS技术的金银花、山银花化学成分比较;李泮霖 等;《中南药学》;20160430;第14卷(第4期);第363-369页 * |
| 硫磺熏蒸金银花中断马钱子酸亚硫酸衍生物产生的机制探讨;郭爱丽 等;《中国中药杂志》;20140531;第39卷(第9期);第1639-1643页 * |
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