CN109022528A - A kind of preparation method of rice high F value oligopeptide - Google Patents
A kind of preparation method of rice high F value oligopeptide Download PDFInfo
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Abstract
A kind of preparation method of rice high F value oligopeptide belongs to foods processing technique, mainly comprise the steps that rice adds water, with milling treatment of colloid, water-bath is simultaneously kept stirring, supernatant is abandoned in centrifugation, it is repeated 3 times, rice after removal of impurities carries out ungrease treatment, precipitation after processing, prepare inverse micellar solution, degreasing rice is weighed to be added in inverse micellar solution, residue is centrifuged off after ultrasonic extraction, KCl buffer is added in forward extraction liquid, it is centrifuged after ultrasonic extraction, supernatant freeze-drying, rice albumen is dissolved in water, adjust pH, alkali protease is added, enzyme deactivation after enzymatic hydrolysis, pH is adjusted after cooling again, flavor protease is added, enzyme deactivation is centrifuged after enzymatic hydrolysis, adjust supernatant pH value, it is adsorbed with active carbon particle, it filters up to rice high F value oligopeptide；The present invention can make full use of rice albumen, and obtain the higher high F value oligopeptide of purity, and then improve the economic benefit of rice.
Description
Technical field
The invention belongs to foods processing techniques, relate generally to a kind of preparation method of rice high F value oligopeptide.
Background technique
Rice is rice by fermenting and producing alcohol, monosodium glutamate, and the by-product left after starch sugar etc., protein content exists
50%-60%, therefore can be used as production of albumen powder, food additives, or preparation have the biologically active peptide of physiological activity.But
It is at present in China generally using rice as animal feed, this greatly wastes good protein resource, therefore how to utilize rice
Egg preserved in wine is white, improves its economic benefit and is a problem to be solved.During the fermentation, it can be produced after rice starch is liquefied, is saccharified
Soluble saccharide is given birth to, but since contents of saccharide is high, therefore understands the glucide of remnant in rice.In recent years, increasingly
More scholars is dedicated to extracting protein from rice because rice protein be largely it is alkali-soluble, alkali can be used
The heavy method of molten acid is extracted to obtain rice protein, but can generate the industrial wastewater of a large amount of alkalinity in this way, causes seriously to environment
Pollution.Currently, big mpd polypeptide is mainly prepared by enzyme process, make certain gains both at home and abroad to the research of big mpd polypeptide, but remote
Go deep into not as good as to other beans polypeptide researchs, and it is domestic also rarely found to its development and utilization.
Oligo-peptide mixture is the mixed small peptide as composed by 2-9 amino acid residue (or oligopeptides) system,
F value is the molal quantity ratio of branched-chain amino acid Yu aromatic amino acid content, and F value is 3.0-3.5 in the blood of normal person, and is suffered from
There is the F value of liver diseases patient there was only 1.0 or lower, the F value of high F value oligopeptide should be greater than 20.Oligopeptides is by 2-9 amino acid
The protein precursor being composed, or the protein degradation object being made of protein degradation to 2-9 amino acid, can also claim small
Peptide, small peptide etc..Since it has, unique amino acid is formed and physiological function, the height for being subjected to food and medicine circle are closed
Note.The present invention first crushes rice with colloid mill, and under this degree of grinding, the soluble impurity in rice can be abundant
It is dissolved into water, and since glutelin not soluble in water in rice accounts for major part, and in saccharifying, soluble protein is big
Part has been dissolved, therefore rice albumen is largely water-insoluble, therefore the albumen lost in water-washing process is considerably less.
It is compared, is had following excellent with the production technology of traditional rice albumen using the technique of inverse micelle abstraction technique preparation rice protein
Point: (1) protein is in extraction process, always in water environment, therefore destroys to its biological characteristics smaller.(2) process
In do not need plus bronsted lowry acids and bases bronsted lowry, and extractant can be recycled, and then solve the problems, such as environmental pollution.(3) it can be improved
The utilization rate of raw material, improves economic benefit.The protein concentrate that rice obtains after pre-treatment is digested by two steps, due to
Different protease has specificity to protein, and action site is also different, therefore can obtain different recovery rate and degree of hydrolysis,
The best hydrolysis effect of rice albumen in order to obtain, the present invention use restriction endonuclease (alkali protease) and excision enzyme (flavor albumen
Enzyme) combination.Enzymatic hydrolysis obtains the mixed liquor containing high F value oligopeptide and amino acid, carries out adsorption treatment with active carbon, can remove wherein
Free amino acid, while decolorizing effect can also be played, to obtain the higher high F value oligopeptide of purity.
In short, soluble impurity in rice can be removed using washed with impurities method first.Utilize inverse micelle abstraction rice egg
White extraction conditions is mild, and the purity of product is higher, the denaturation degrees very little of protein.Then it is digested, is obtained containing high F using two steps
It is worth the mixed liquor of oligopeptides and amino acid, finally uses activated carbon adsorption, the higher high F value oligopeptide of purity can be obtained.It in this way can be with
Rice albumen is made full use of, its economic benefit is improved.
Summary of the invention
The technical problem to be solved by the present invention is to overcome above-mentioned the deficiencies in the prior art, it is few to provide a kind of rice high F value
The preparation method of peptide reaches and substantially effectively extracts rice albumen, digests to obtain the higher high F value oligopeptide of purity by two steps
Effect, realize improve rice economic value purpose.
The technical problem to be solved by the present invention is to what is be achieved through the following technical solutions:
(1) washed with impurities method: adding water to rice raw material, then uses milling treatment of colloid, heating water bath and protects after processing
Stirring 15min is held, removes supernatant after centrifugation, the mass ratio of the rice raw material and water is 1:5；
(2) preparation of degreasing rice: the rice after removal of impurities is handled in carrying out ungrease treatment with petroleum ether in Soxhlet extraction device
After be placed on ventilation carry out precipitation；
(3) isooctane, the addition isooctane to concentration the preparation of inverse micellar solution: are added into AOT surfactant
For 0.05~0.15g/mL, magnetic agitation is completely dissolved surfactant, Kcl solution is added into solution, the Kcl's is dense
Degree is 0.1mol/L, and additive amount is 10 times of surfactant solution volume, is then centrifuged 20min under the conditions of 2000r/min,
Obtained transparent clear system is inverse micellar solution；
(4) inverse micellar solution extract rice albumen: by degreasing rice be added inverse micellar solution in, until concentration be 0.01~
0.02g/ml, Wo value are 18, carry out ultrasonic extraction processing, and the ultrasonic power is 250W, and extraction temperature is 40 DEG C, when extraction
Between be 20~30min, extraction pH is 7, is centrifuged 15min after extraction under the conditions of 4000r/min, remove residue and obtain extract liquor,
Kcl buffer is added into extract liquor, the supernatant for carrying out being centrifuged after ultrasonic extraction again carries out frozen dried and obtains rice
Egg preserved in wine is white；
(5) two steps enzymatic hydrolysis: rice albumen being dissolved in water, the mass fraction 10%~15% of rice albumen is made, and is adjusted
PH to 8.5-9.5, be added flavor protease carry out enzymolysis processing, the hydrolysis temperature be 55-60 DEG C, enzymolysis time be 2~
3h, enzyme deactivation after enzymatic hydrolysis adjust pH to be transferred to 4~6 again after cooling, flavor protease is added and is digested, and the hydrolysis temperature is 40-
50 DEG C, enzymolysis time is 3~4h, and enzyme deactivation is centrifuged 10~20min under the conditions of 4000~5000r/min after enzymatic hydrolysis, is removed after centrifugation
It goes residue to obtain supernatant, adjusts supernatant pH value to 3~4, adsorbed with active carbon particle, the quality and activity of the material
The volume ratio of charcoal particle is 10~20:1,10~20min of adsorption time, is filtered after absorption up to rice high F value oligopeptide.
The higher high F value oligopeptide of rice high F value oligopeptide purity prepared with this method, and rice egg can be made full use of
It is white, improve its economic benefit.
Detailed description of the invention
The process flow of Fig. 1 preparation rice high F value oligopeptide.
Specific embodiment
Below with reference to Fig. 1, the present invention will be described in detail,
(1) appropriate rice raw material 100g is accurately weighed, adds water in 1:5 ratio, with milling treatment of colloid, makes rice particle diameter
Size is 5~50 μm and is uniformly distributed in water phase, water-bath 15min under certain temperature, and is kept stirring, and is centrifuged (2 later
000r/min 15min), liquid is discarded supernatant, is repeated 3 times.(2) rice after cleaning is at 50 DEG C in using petroleum in Soxhlet extraction device
Ether carries out ungrease treatment, and ventilation is placed on after processing and carries out precipitation, keeps petroleum ether volatilization complete.(3) surface AOT of 5g is weighed
Activating agent is put into 1000mL beaker, and it is 0.10g/mL that isooctane to concentration, which is added, and magnetic agitation keeps surfactant completely molten
Solution, into solution, the KCl solution of 0.1mol/L is added in 1:10 by volume, is then centrifuged 20min under the conditions of 2000r/min,
Obtained transparent clear system is inverse micellar solution (a small amount of water of bottom influences system little).(4) the anti-of 500mL is weighed
Micellar solution is incorporated in beaker, weighs in 10g degreasing rice addition inverse micellar solution and (is accurate to 0.0001g), and Wo value is 18, with
The ultrasonic power of 250W is handled, and extraction temperature is 40 DEG C, 20~30min of extraction time, pH value 7, then in 4000r/min item
It is centrifuged 15min under part, removes residue.(5) 200mL forward extraction liquid is added to isometric 1.0mol/L KCl buffer, with 270W
Ultrasonic power processing, extraction temperature be 30 DEG C, 20~30min of extraction time, pH value 9, then under the conditions of 4000r/min
It is centrifuged 15min, supernatant freeze-drying.(6) plus distilled water makes the content 10% of rice albumen, with 0.1mol/L HCl or
0.1mol/L NaOH adjusts pH to 9.0, and alkali protease, the enzyme at 55 DEG C is added according to the 1.0%~2.0% of albumen quality
Solution reaction 3h, enzymolysis liquid heat 10min enzyme deactivation at 90 DEG C, and pH is transferred to 5 after cooling, according to the 0.5%~1.0% of albumen quality
Flavor protease, the enzyme digestion reaction 4h at 50 DEG C is added, enzymolysis liquid heats 10min enzyme deactivation at 90 DEG C, adjusts pH to arrive after cooling
4.0,15min is centrifuged under the conditions of 4500r/min.(7) supernatant pH value is adjusted to 4, the quality and active carbon particle of material
Volume ratio be 10:1, adsorption time 20min.
Embodiment 1:
(1) appropriate rice raw material 100g is accurately weighed, adds water in 1:5 ratio, with milling treatment of colloid, makes rice particle diameter
Size is 5~50 μm and is uniformly distributed in water phase, water-bath 15min under certain temperature, and is kept stirring, and is centrifuged (2 later
000r/min 15min), liquid is discarded supernatant, is repeated 3 times.(2) rice after cleaning is at 50 DEG C in using petroleum in Soxhlet extraction device
Ether carries out ungrease treatment, and ventilation is placed on after processing and carries out precipitation, keeps petroleum ether volatilization complete.(3) surface AOT of 5g is weighed
Activating agent is put into 1000mL beaker, and it is 0.10g/mL that isooctane to concentration, which is added, and magnetic agitation keeps surfactant completely molten
Solution, into solution, the KCl solution of 0.1mol/L is added in 1:10 by volume, is then centrifuged 20min under the conditions of 2000r/min,
Obtained transparent clear system is inverse micellar solution (a small amount of water of bottom influences system little).(4) the anti-of 500mL is weighed
Micellar solution is incorporated in beaker, weighs in 10g degreasing rice addition inverse micellar solution and (is accurate to 0.0001g), and Wo value is 18, with
The ultrasonic power of 250W is handled, and extraction temperature is 40 DEG C, extraction time 30min, pH value 7, then under the conditions of 4000r/min
It is centrifuged 15min, removes residue.(5) 200mL forward extraction liquid is added to isometric 1.0mol/L KCl buffer, with the super of 270W
Acoustical power processing, extraction temperature are 30 DEG C, extraction time 30min, then pH value 9 is centrifuged under the conditions of 4000r/min
15min, supernatant freeze-drying.(6) distilled water is added to make the content 10% of rice albumen, with 0.1mol/L HCl or 0.1mol/L
NaOH adjusts pH to 9.0, alkali protease is added according to the 1.0% of albumen quality, enzyme digestion reaction 3h, enzymolysis liquid exist at 55 DEG C
10min enzyme deactivation is heated at 90 DEG C, pH is transferred to 5 after cooling, flavor protease is added according to the 0.5% of albumen quality, at 50 DEG C
Enzyme digestion reaction 4h, enzymolysis liquid heat 10min enzyme deactivation at 90 DEG C, adjust pH to 4.0 after cooling, be centrifuged under the conditions of 4500r/min
15min.(7) supernatant pH value is adjusted to 4, the quality of material and the volume ratio of active carbon particle are 10:1, adsorption time
20min。
The final protein matter rate of recovery is 93.4%, and the peptide yield of high F value oligopeptide is 64.7%.
Embodiment 2:
(1) appropriate rice raw material 100g is accurately weighed, adds water in 1:5 ratio, with milling treatment of colloid, makes rice particle diameter
Size is 5~50 μm and is uniformly distributed in water phase, water-bath 15min under certain temperature, and is kept stirring, and is centrifuged (2 later
000r/min 15min), liquid is discarded supernatant, is repeated 3 times.(2) rice after cleaning is at 50 DEG C in using petroleum in Soxhlet extraction device
Ether carries out ungrease treatment, and ventilation is placed on after processing and carries out precipitation, keeps petroleum ether volatilization complete.(3) surface AOT of 5g is weighed
Activating agent is put into 1000mL beaker, and it is 0.10g/mL that isooctane to concentration, which is added, and magnetic agitation keeps surfactant completely molten
Solution, into solution, the KCl solution of 0.1mol/L is added in 1:10 by volume, is then centrifuged 20min under the conditions of 2000r/min,
Obtained transparent clear system is inverse micellar solution (a small amount of water of bottom influences system little).(4) the anti-of 500mL is weighed
Micellar solution is incorporated in beaker, weighs in 10g degreasing rice addition inverse micellar solution and (is accurate to 0.0001g), and Wo value is 18, with
The ultrasonic power of 250W is handled, and extraction temperature is 40 DEG C, extraction time 20min, pH value 7, then under the conditions of 4000r/min
It is centrifuged 15min, removes residue.(5) 200mL forward extraction liquid is added to isometric 1.0mol/L KCl buffer, with the super of 270W
Acoustical power processing, extraction temperature are 30 DEG C, extraction time 20min, then pH value 9 is centrifuged under the conditions of 4000r/min
15min, supernatant freeze-drying.(6) distilled water is added to make the content 10% of rice albumen, with 0.1mol/L HCl or 0.1mol/L
NaOH adjusts pH to 9.0, alkali protease is added according to the 1.0% of albumen quality, enzyme digestion reaction 3h, enzymolysis liquid exist at 55 DEG C
10min enzyme deactivation is heated at 90 DEG C, pH is transferred to 5 after cooling, flavor protease is added according to the 0.5% of albumen quality, at 50 DEG C
Enzyme digestion reaction 4h, enzymolysis liquid heat 10min enzyme deactivation at 90 DEG C, adjust pH to 4.0 after cooling, be centrifuged under the conditions of 4500r/min
15min.(7) supernatant pH value is adjusted to 4, the quality of material and the volume ratio of active carbon particle are 10:1, adsorption time
20min。
The final protein matter rate of recovery is 85.6%, and the peptide yield of high F value oligopeptide is 51.0%.
Embodiment 3:
(1) appropriate rice raw material 100g is accurately weighed, adds water in 1:5 ratio, with milling treatment of colloid, makes rice particle diameter
Size is 5~50 μm and is uniformly distributed in water phase, water-bath 15min under certain temperature, and is kept stirring, and is centrifuged (2 later
000r/min 15min), liquid is discarded supernatant, is repeated 3 times.(2) rice after cleaning is at 50 DEG C in using petroleum in Soxhlet extraction device
Ether carries out ungrease treatment, and ventilation is placed on after processing and carries out precipitation, keeps petroleum ether volatilization complete.(3) surface AOT of 5g is weighed
Activating agent is put into 1000mL beaker, and it is 0.10g/mL that isooctane to concentration, which is added, and magnetic agitation keeps surfactant completely molten
Solution, into solution, the KCl solution of 0.1mol/L is added in 1:10 by volume, is then centrifuged 20min under the conditions of 2000r/min,
Obtained transparent clear system is inverse micellar solution (a small amount of water of bottom influences system little).(4) the anti-of 500mL is weighed
Micellar solution is incorporated in beaker, weighs in 10g degreasing rice addition inverse micellar solution and (is accurate to 0.0001g), and Wo value is 18, with
The ultrasonic power of 250W is handled, and extraction temperature is 40 DEG C, extraction time 30min, pH value 7, then under the conditions of 4000r/min
It is centrifuged 15min, removes residue.(5) 200mL forward extraction liquid is added to isometric 1.0mol/L KCl buffer, with the super of 270W
Acoustical power processing, extraction temperature are 30 DEG C, extraction time 30min, then pH value 9 is centrifuged under the conditions of 4000r/min
15min, supernatant freeze-drying.(6) distilled water is added to make the content 10% of rice albumen, with 0.1mol/L HCl or 0.1mol/L
NaOH adjusts pH to 9.0, alkali protease is added according to the 2.0% of albumen quality, enzyme digestion reaction 3h, enzymolysis liquid exist at 55 DEG C
10min enzyme deactivation is heated at 90 DEG C, pH is transferred to 5 after cooling, flavor protease is added according to the 1.0% of albumen quality, at 50 DEG C
Enzyme digestion reaction 4h, enzymolysis liquid heat 10min enzyme deactivation at 90 DEG C, adjust pH to 4.0 after cooling, be centrifuged under the conditions of 4500r/min
15min.(7) supernatant pH value is adjusted to 4, the quality of material and the volume ratio of active carbon particle are 10:1, adsorption time
20min。
The final protein matter rate of recovery is 93.4%, and the peptide yield of high F value oligopeptide is 68.4%.
Claims (1)
1. a kind of preparation method of rice high F value oligopeptide, which comprises the following steps:
(1) washed with impurities method: adding water to rice raw material, then use milling treatment of colloid, after processing heating water bath and keep stir
15min is mixed, removes supernatant after centrifugation, the mass ratio of the rice raw material and water is 1:5；
(2) preparation of degreasing rice: the rice after removal of impurities is put after processing in carrying out ungrease treatment with petroleum ether in Soxhlet extraction device
It sets and carries out precipitation in ventilation；
(3) preparation of inverse micellar solution: isooctane being added into AOT surfactant, and the addition isooctane to concentration is
0.05~0.15g/mL, magnetic agitation are completely dissolved surfactant, and Kcl solution, the concentration of the Kcl are added into solution
For 0.1mol/L, additive amount is 10 times of surfactant solution volume, is then centrifuged 20min under the conditions of 2000r/min, obtains
To transparent clear system be inverse micellar solution；
(4) inverse micellar solution extracts rice albumen: degreasing rice being added in inverse micellar solution, until concentration is 0.01~0.02g/
Ml, Wo value are 18, carry out ultrasonic extraction processing, and the ultrasonic power is 250W, and extraction temperature is 40 DEG C, extraction time 20
~30min, extraction pH are 7, are centrifuged 15min after extraction under the conditions of 4000r/min, remove residue and obtain extract liquor, to extraction
Kcl buffer is added in liquid, the supernatant for carrying out being centrifuged after ultrasonic extraction again carries out frozen dried and obtains rice albumen；
(5) two steps enzymatic hydrolysis: rice albumen is dissolved in water, and makes the mass fraction 10%~15% of rice albumen, is adjusted pH and is arrived
8.5-9.5 is added flavor protease and carries out enzymolysis processing, and the hydrolysis temperature is 55-60 DEG C, and enzymolysis time is 2~3h, enzyme
Enzyme deactivation after solution adjusts pH to be transferred to 4~6 again after cooling, flavor protease is added and is digested, and the hydrolysis temperature is 40-50 DEG C,
Enzymolysis time is 3~4h, and enzyme deactivation is centrifuged 10~20min under the conditions of 4000~5000r/min after enzymatic hydrolysis, removes residue after centrifugation
Supernatant is obtained, supernatant pH value is adjusted to 3~4, is adsorbed with active carbon particle, the quality and active carbon particle of the material
Volume ratio be 10~20:1,10~20min of adsorption time, filtered after absorption up to rice high F value oligopeptide.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003192695A (en) * | 2001-12-27 | 2003-07-09 | National Institute Of Advanced Industrial & Technology | Angiotensin i converting enzyme inhibitor |
| CN101096698A (en) * | 2007-07-18 | 2008-01-02 | 东北农业大学 | Preparation technology for soybean oligopeptide with high F-value |
| CN101402670A (en) * | 2008-11-14 | 2009-04-08 | 江南大学 | Method simultaneously extracting lipid and protein from corn plantule |
| CN105441520A (en) * | 2015-11-23 | 2016-03-30 | 华中农业大学 | Method adopting rice residues as raw material for enzyme-membrane combined preparation of rice polypeptides |
| CN105639048A (en) * | 2016-01-12 | 2016-06-08 | 华隆（乳山）食品工业有限公司 | Industrial production method of high-F-value corn oligopeptide with alcohol dispelling and liver protection activities |
| CN105779114A (en) * | 2014-12-22 | 2016-07-20 | 丰益(上海)生物技术研发中心有限公司 | Method for extraction of rice bran with reverse micelle solvent |
| CN107602659A (en) * | 2017-09-15 | 2018-01-19 | 大连工业大学 | It is a kind of to be used to extract reverse micelle system of protein and its preparation method and application |
-
2018
- 2018-08-30 CN CN201810999162.9A patent/CN109022528B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003192695A (en) * | 2001-12-27 | 2003-07-09 | National Institute Of Advanced Industrial & Technology | Angiotensin i converting enzyme inhibitor |
| CN101096698A (en) * | 2007-07-18 | 2008-01-02 | 东北农业大学 | Preparation technology for soybean oligopeptide with high F-value |
| CN101402670A (en) * | 2008-11-14 | 2009-04-08 | 江南大学 | Method simultaneously extracting lipid and protein from corn plantule |
| CN105779114A (en) * | 2014-12-22 | 2016-07-20 | 丰益(上海)生物技术研发中心有限公司 | Method for extraction of rice bran with reverse micelle solvent |
| CN105441520A (en) * | 2015-11-23 | 2016-03-30 | 华中农业大学 | Method adopting rice residues as raw material for enzyme-membrane combined preparation of rice polypeptides |
| CN105639048A (en) * | 2016-01-12 | 2016-06-08 | 华隆（乳山）食品工业有限公司 | Industrial production method of high-F-value corn oligopeptide with alcohol dispelling and liver protection activities |
| CN107602659A (en) * | 2017-09-15 | 2018-01-19 | 大连工业大学 | It is a kind of to be used to extract reverse micelle system of protein and its preparation method and application |
Non-Patent Citations (4)
| Title |
|---|
| HONGBIN WANG等: "Preparing oligopeptides from broken rice protein by ultrafiltration-coupled enzymatic hydrolysis", 《EUROPEAN FOOD RESEARCH AND TECHNOLOGY》 * |
| 华从伶: "米糟蛋白的提取及制备高F值寡肽的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 肖信锦等: "超声波辅助SDS反胶束体系前萃米糠蛋白的工艺研究", 《中国油脂》 * |
| 赵丰丽等: "米糟蛋白酶解制备高F值寡肽的研究", 《中国酿造》 * |
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