CN109022295A - A method of utilizing white rot fungus degrading Nitenpyram - Google Patents
A method of utilizing white rot fungus degrading Nitenpyram Download PDFInfo
- Publication number
- CN109022295A CN109022295A CN201810999741.3A CN201810999741A CN109022295A CN 109022295 A CN109022295 A CN 109022295A CN 201810999741 A CN201810999741 A CN 201810999741A CN 109022295 A CN109022295 A CN 109022295A
- Authority
- CN
- China
- Prior art keywords
- nitenpyram
- white
- rot fungus
- fungus degrading
- white rot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental & Geological Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Medicinal Chemistry (AREA)
Abstract
The present invention relates to a kind of methods using white rot fungus degrading Nitenpyram, belong to microorganisms technical field.Method using white rot fungus degrading Nitenpyram of the invention, comprising the following steps: (1) White-Rot Fungi YK-624 is cultivated in PDA culture medium (potato glucose agar medium), is placed in refrigerator and is saved backup for 4 DEG C；(2) after mycelia is covered with, interception diameter be 10mm white-rot fungi mycelia piece be inoculated in Kirk fluid nutrient medium, in 24~30 DEG C stationary culture 3~5 days；(3) be added 0.05~0.15mM Nitenpyram mother liquor, in 24~30 DEG C continuation stationary culture 3~5 days.The present invention using white-rot fungi is small in size, large specific surface area, fertility are strong, the advantages such as adaptable, the method using white rot fungus degrading Nitenpyram of exploitation have the advantages that degradation rate it is high, safely, risk without secondary pollution.
Description
Technical field
The present invention relates to a kind of methods using white rot fungus degrading Nitenpyram, belong to microorganisms technical field.
Background technique
Nitenpyram (nitenpyram, NIT), entitled (E)-N- (6- the chloro-3-pyridylmethyl)-N- ethyl-N '-first of chemistry
Base -2- nitro ethylene diamine, is one kind of anabasine insecticide.Anabasine insecticide is that organophosphorus pesticide is replaced to exist
In generation nineteen ninety, is developed, and world in recent years various regions are widely used a kind of efficient, highly selective novel pesticide.Anabasine
Insecticide pass through exciting postsynaptic membrane nicotinic acetylcholine receptor (nicotinic acetylcholine receptors,
NAChR), block insect CNS normal conduction and make its death.And in mammals, anabasine insecticide is worn
The ability of saturating blood-brain barrier is poor, and weaker with the nAChR of central nervous system and peripheral neverous system effect, therefore to lactation
Animal toxicity is low.Because of its unique mechanism of action, so that anabasine insecticide becomes with fastest developing speed, sale in biocides market
One of the most successful, kind that insecticidal effect is best.Since imidacloprid listing since 1991, anabasine insecticide grows rapidly
As global first big insecticide, usage amount also increases every year.However, more and more evidences are shown now, anabasine
The use of insecticide can cause bee colony to collapse syndrome, cause honeybee missing and mortality.Recently again studies have found that, should
Insecticides also have toxicity to aquatic animal and mammal, including the mankind.European Food Safety Authority also reports two kinds
Anabasine insecticide Acetamiprid and imidacloprid may impact developmental human nervous system.If continued from now on big
Amount uses, and refractory organics will pollute soil and river, it is possible to can become " second DDT ".With various
The continuous disclosure of experimental data, national governments are also in the continuous decree or measure promulgated for anabasine insecticide: European Union exists
It announces within 2013 temporarily to forbid use of three kinds of leading anabasine insecticides on certain crops；2017, European Union's May
Question disables anabasine insecticide decree；Also anabasine insecticide imidacloprid is stopped using in plan for Canada；France
Will in September, 2018 disabling anabasine insecticide.The residual contamination problem and toxic mechanism of anabasine insecticide are ground
Study carefully and causes mondial extensive concern.
Less to the degradation report of Nitenpyram both at home and abroad, Li Shanping generates low temperature plasma by dielectric barrier discharge
Nitenpyram solution is handled, different medium barrier discharge power and extraneous factor such as Fe are had studied2+, n-butanol, inorganic salts
Na2CO3And H2O2The influence of equal plasmas degradation Nitenpyram is (useless using low-temperature plasma degradation Nitenpyram pesticide
The research of water, " High-Voltage Technology ", 10 phases in 2011)；Lian Junfeng passes through Nitenpyram in preparation gas-diffusion electrode degradation water,
Using Star point design -- effect surface curve method is useless to the preparation of catalyst, the preparation of gas-diffusion electrode and Nitenpyram pesticide
(preparation of gas-diffusion electrode and Nitenpyram agricultural chemicals waste water mechanism of degradation, master's degree is optimized in the degradation technique of water
Paper, 2011 year of Shandong University).Material preparation cost used in the above method is high, and operational management is cumbersome, and easily makes
At secondary pollution.
White-rot fungi plays a significant role in the nature ecosystem, is a kind of living resources of preciousness, is that environment is controlled
The tool and research mode object of reason.White-rot fungi is not required to the fore condition by specific pollutants before Recalcitrant chemicals of degrading
Change, the different pollutant of a large amount of structures that can degrade, and low to nutritional condition requirement, cost can be saved.White-rot fungi
Phanerochaete sordida YK-624 has higher than mode the ability of numerous Recalcitrant chemicals such as aflatoxin
Bacterial strain Phanerochaete chrysosporium.White-rot fungi shows wide prospect in processing organic pollutant, makes full use of white rot true
Bacterium is to the realistic meaning of environment remediation, so that the bioremediation technology for development and utilization microbial degradation anabasine insecticide mentions
For theoretical foundation.
Summary of the invention
It is provided it is an object of the invention to overcome in place of above-mentioned the deficiencies in the prior art and a kind of utilizes white rot fungus degrading
The method of Nitenpyram, large specific surface area small in size using white-rot fungi, fertility be strong, the advantages such as adaptable, exploitation
Have the advantages that degradation rate height, safety, risk without secondary pollution using the method for white rot fungus degrading Nitenpyram.
To achieve the above object, the technical scheme adopted by the invention is as follows: it is a kind of using white rot fungus degrading Nitenpyram
Method, comprising the following steps:
(1) White-Rot Fungi YK-624 is placed in refrigerator and saves backup for 4 DEG C in PDA culture medium culture；
(2) after mycelia is covered with, the white-rot fungi mycelia piece that interception diameter is 10mm is inoculated in Kirk fluid nutrient medium,
In 24~30 DEG C stationary culture 3~5 days；
(3) be added 0.05~0.15mM Nitenpyram mother liquor, in 24~30 DEG C continuation stationary culture 3~5 days.
As the preferred embodiment of the method for the present invention using white rot fungus degrading Nitenpyram, the Kirk liquid
The ingredient of body culture medium are as follows: 10g glucose, 0.221g ammonium tartrate, 1.64g anhydrous sodium acetate, 100mL salting liquid plus water are fixed
Hold to 1L.
As the preferred embodiment of the method for the present invention using white rot fungus degrading Nitenpyram, the salting liquid
Ingredient are as follows: 20g KH2PO4、5g MgSO4·7H2O、1.3g CaCl2·2H2O, the micro member of 0.01g Tyiamine Hd, 16.7mL
Plain solution, adds water to be settled to 1L.
As the preferred embodiment of the method for the present invention using white rot fungus degrading Nitenpyram, the micro member
The ingredient of plain solution are as follows: 9g Nitrilotriacetate, 3g MgSO4·7H2O、4.2g MnSO4·H2O、6g NaCl、
0.6g FeSO4·7H2O、1.1g CoSO4·7H2O、1.1g ZnSO4·7H2O、0.6g CaCl2·2H2O、0.06g
CuSO4·5H2O、0.11g AlK(SO4)2·12H2O、0.06g H3BO3、0.07g Na2MoO4·2H2O adds water to be settled to 1L.
As the preferred embodiment of the method for the present invention using white rot fungus degrading Nitenpyram, the step
(1) in, PDA culture medium includes the component of following mass percents: potato 20%, glucose 2%, agar 2%, water surplus.
As the preferred embodiment of the method for the present invention using white rot fungus degrading Nitenpyram, the step
(2) in, Kirk fluid nutrient medium is 10mL.
As the preferred embodiment of the method for the present invention using white rot fungus degrading Nitenpyram, the step
(2) in, cultivation temperature is 30 DEG C, and incubation time is 5 days.
As the preferred embodiment of the method for the present invention using white rot fungus degrading Nitenpyram, the step
(3) in, cultivation temperature is 30 DEG C, and incubation time is 5 days.
As the preferred embodiment of the method for the present invention using white rot fungus degrading Nitenpyram, the step
(3) in, the concentration of Nitenpyram mother liquor is 0.1mM.
Compared with prior art, the invention has the benefit that the present invention is using white-rot fungi is small in size, specific surface area
Greatly, fertility is strong, the advantages such as adaptable, and the method using white rot fungus degrading Nitenpyram of exploitation has degradation rate
The advantages of high, safety, risk without secondary pollution.
Detailed description of the invention
Fig. 1 is structural formula figure of the present invention using the metabolite of white rot fungus degrading Nitenpyram.
Fig. 2 is ESI-TOF-MS mass spectrogram of the present invention using the metabolite of white rot fungus degrading Nitenpyram.
Fig. 3 is metabolite of the present invention using white rot fungus degrading Nitenpyram13C-NMR map.
Fig. 4 is metabolite of the present invention using white rot fungus degrading Nitenpyram1H-NMR map.
Specific embodiment
Purposes, technical schemes and advantages in order to better illustrate the present invention, below in conjunction with the drawings and specific embodiments pair
The present invention is described further.
Embodiment 1
The present invention utilizes a kind of embodiment of the method for white rot fungus degrading Nitenpyram, comprising the following steps:
(1) White-Rot Fungi YK-624 is placed in refrigerator and saves backup for 4 DEG C in PDA culture medium culture；
(2) after mycelia is covered with, the white-rot fungi mycelia piece that interception diameter is 10mm is inoculated in 10mL Kirk Liquid Culture
In base, in 30 DEG C stationary culture 5 days；
(3) be added 0.1mM Nitenpyram mother liquor, in 30 DEG C continuation stationary culture 5 days.
Wherein, the ingredient of Kirk fluid nutrient medium are as follows: 10g glucose, 0.221g ammonium tartrate, 1.64g anhydrous sodium acetate,
100mL salting liquid, Jia Shui are settled to 1L；The ingredient of salting liquid are as follows: 20g KH2PO4、5g MgSO4·7H2O、1.3g CaCl2·
2H2O, 0.01g Tyiamine Hd, 16.7mL trace element solution, add water to be settled to 1L；The ingredient of trace element solution are as follows: 9g
Nitrilotriacetate、3g MgSO4·7H2O、4.2g MnSO4·H2O、6g NaCl、0.6g FeSO4·7H2O、1.1g
CoSO4·7H2O、1.1g ZnSO4·7H2O、0.6g CaCl2·2H2O、0.06g CuSO4·5H2O、0.11g AlK
(SO4)2·12H2O、0.06g H3BO3、0.07g Na2MoO4·2H2O adds water to be settled to 1L.PDA culture medium includes following quality
The component of percentage: potato 20%, glucose 2%, agar 2%, water surplus.
Embodiment 2
The present invention utilizes a kind of embodiment of the method for white rot fungus degrading Nitenpyram, comprising the following steps:
(1) White-Rot Fungi YK-624 is placed in refrigerator and saves backup for 4 DEG C in PDA culture medium culture；
(2) after mycelia is covered with, the white-rot fungi mycelia piece that interception diameter is 10mm is inoculated in 10mL Kirk Liquid Culture
In base, in 28 DEG C stationary culture 3 days；
(3) be added 0.05mM Nitenpyram mother liquor, in 28 DEG C continuation stationary culture 3 days.
Wherein, the ingredient of Kirk fluid nutrient medium are as follows: 10g glucose, 0.221g ammonium tartrate, 1.64g anhydrous sodium acetate,
100mL salting liquid, Jia Shui are settled to 1L；The ingredient of salting liquid are as follows: 20g KH2PO4、5g MgSO4·7H2O、1.3g CaCl2·
2H2O, 0.01g Tyiamine Hd, 16.7mL trace element solution, add water to be settled to 1L；The ingredient of trace element solution are as follows: 9g
Nitrilotriacetate、3g MgSO4·7H2O、4.2g MnSO4·H2O、6g NaCl、0.6g FeSO4·7H2O、1.1g
CoSO4·7H2O、1.1g ZnSO4·7H2O、0.6g CaCl2·2H2O、0.06g CuSO4·5H2O、0.11g AlK
(SO4)2·12H2O、0.06g H3BO3、0.07g Na2MoO4·2H2O adds water to be settled to 1L.PDA culture medium includes following quality
The component of percentage: potato 20%, glucose 2%, agar 2%, water surplus.
Embodiment 3
The present invention utilizes a kind of embodiment of the method for white rot fungus degrading Nitenpyram, comprising the following steps:
(1) White-Rot Fungi YK-624 is placed in refrigerator and saves backup for 4 DEG C in PDA culture medium culture；
(2) after mycelia is covered with, the white-rot fungi mycelia piece that interception diameter is 10mm is inoculated in 10mL Kirk Liquid Culture
In base, in 24 DEG C stationary culture 5 days；
(3) be added 0.15mM Nitenpyram mother liquor, in 24 DEG C continuation stationary culture 5 days.
Wherein, the ingredient of Kirk fluid nutrient medium are as follows: 10g glucose, 0.221g ammonium tartrate, 1.64g anhydrous sodium acetate,
100mL salting liquid, Jia Shui are settled to 1L；The ingredient of salting liquid are as follows: 20gKH2PO4、5g MgSO4·7H2O、1.3g CaCl2·
2H2O, 0.01g Tyiamine Hd, 16.7mL trace element solution, add water to be settled to 1L；The ingredient of trace element solution are as follows: 9g
Nitrilotriacetate、3g MgSO4·7H2O、4.2g MnSO4·H2O、6g NaCl、0.6g FeSO4·7H2O、1.1g
CoSO4·7H2O、1.1g ZnSO4·7H2O、0.6g CaCl2·2H2O、0.06g CuSO4·5H2O、0.11g AlK
(SO4)2·12H2O、0.06g H3BO3、0.07g Na2MoO4·2H2O adds water to be settled to 1L.PDA culture medium includes following quality
The component of percentage: potato 20%, glucose 2%, agar 2%, water surplus.
Comparative example 1
A method of utilizing white rot fungus degrading Nitenpyram, comprising the following steps:
(1) White-Rot Fungi YK-624 is placed in refrigerator and saves backup for 4 DEG C in PDA culture medium culture；
(2) after mycelia is covered with, the white-rot fungi mycelia piece that interception diameter is 10mm is inoculated in 10mL PDB Liquid Culture
In base (potato dextrose broth), in 30 DEG C stationary culture 5 days；
(3) be added 0.1mM Nitenpyram mother liquor, in 30 DEG C continuation stationary culture 5 days.
Wherein, the ingredient of PDB fluid nutrient medium are as follows: potato 20%, glucose 2%, water surplus.Under PDA culture medium includes
State the component of mass percent: potato 20%, glucose 2%, agar 2%, water surplus.
Effect example 1
The embodiment of the present invention 1~3 and comparative example 1 test the degradation rate of Nitenpyram
After the completion of the degradation experiment of example 1~3 and comparative example 1 to be performed, in the culture solution of Examples 1 to 3 and comparative example 1
Internal standard compound is added, smashes thallus by hand held tissue homogenizer after 20mL acetone is added.Steaming is rotated after obtaining homogenate filtering
Hair concentration carries out high performance liquid chromatography (HPLC, chromatographic column: 5 μm of 4.6x250mm of Inertsil ODS-3) analysis.It utilizes
Internal standard method carries out quantitative analysis to Nitenpyram, the results show that Nitenpyram is completely degraded in Examples 1 to 3, and comparative example
The degradation rate of Nitenpyram is only 20% in 1.As it can be seen that degrading using method of the invention to Nitenpyram, there is degradation
The advantages of rate height, safety, risk without secondary pollution.
Effect example 2
The identification of the metabolite of the degradation Nitenpyram of the embodiment of the present invention 1~3
In order to identify the metabolite of Nitenpyram, 148 μM of Nitenpyram are added in 5L fluid nutrient medium, by degradation
Experiment condition culture.Rotation is concentrated by evaporation to 250mL after culture 20 days, is obtained with ethyl acetate extraction 3 times of equal volume
Supernatant, solution extracted are evaporated under reduced pressure in rotary evaporator.Filtrate carries out gradient elution, thin layer with silica gel column chromatography
Chromatography carries out the tracking of metabolite, HPLC (chromatographic column: 5 μm of 4.6x250mm of Inertsil C30S-Select) analysis
Purity.Isolated product is by preparing HPLC (chromatographic column: 5 μm of 20x250mm of Inertsil C30S-Select) into one
One-step refining purifying, obtains the metabolite of high-purity.Metabolism is identified using electrospray ionization mass spectrum and magnetic resonance spectroscopy to produce
Object is (E)-N- ((6-chloropyridin-3-yl) methyl)-N-ethyl-N '-hydroxyacetimidamide
(CPMHA), structural formula such as Fig. 1, ESI-TOF-MS mass spectrum such as Fig. 2,13C-NMR map such as Fig. 3,1H-NMR map such as Fig. 4.
The Nitenpyram as it can be seen that method that the present invention utilizes white rot fungus degrading Nitenpyram can effectively degrade, degradation produce
Object safety, risk without secondary pollution.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of range is protected, although the invention is described in detail with reference to the preferred embodiments, those skilled in the art should
Understand, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the essence of technical solution of the present invention
And range.
Claims (9)
1. a kind of method using white rot fungus degrading Nitenpyram, which comprises the following steps:
(1) White-Rot Fungi YK-624 is placed in refrigerator and saves backup for 4 DEG C in PDA culture medium culture；
(2) after mycelia is covered with, the white-rot fungi mycelia piece that interception diameter is 10mm is inoculated in Kirk fluid nutrient medium, in 24
~30 DEG C stationary culture 3~5 days；
(3) be added 0.05~0.15mM Nitenpyram mother liquor, in 24~30 DEG C continuation stationary culture 3~5 days.
2. utilizing the method for white rot fungus degrading Nitenpyram as described in claim 1, which is characterized in that the Kirk liquid
The ingredient of culture medium are as follows: 10g glucose, 0.221g ammonium tartrate, 1.64g anhydrous sodium acetate, 100mL salting liquid add water constant volume
To 1L.
3. utilizing the method for white rot fungus degrading Nitenpyram as claimed in claim 2, which is characterized in that the salting liquid
Ingredient are as follows: 20g KH2PO4、5g MgSO4·7H2O、1.3g CaCl2·2H2O, 0.01g Tyiamine Hd, 16.7mL microelement
Solution adds water to be settled to 1L.
4. utilizing the method for white rot fungus degrading Nitenpyram as claimed in claim 3, which is characterized in that the microelement
The ingredient of solution are as follows: 9g Nitrilotriacetate, 3g MgSO4·7H2O、4.2g MnSO4·H2O、6g NaCl、
0.6gFeSO4·7H2O、1.1g CoSO4·7H2O、1.1g ZnSO4·7H2O、0.6g CaCl2·2H2O、0.06g CuSO4·
5H2O、0.11g AlK(SO4)2·12H2O、0.06g H3BO3、0.07g Na2MoO4·2H2O adds water to be settled to 1L.
5. utilizing the method for white rot fungus degrading Nitenpyram as described in claim 1, which is characterized in that the step (1)
In, PDA culture medium includes the component of following mass percents: potato 20%, glucose 2%, agar 2%, water surplus.
6. utilizing the method for white rot fungus degrading Nitenpyram as described in claim 1, which is characterized in that the step (2)
In, Kirk fluid nutrient medium is 10mL.
7. utilizing the method for white rot fungus degrading Nitenpyram as described in claim 1, which is characterized in that the step (2)
In, cultivation temperature is 30 DEG C, and incubation time is 5 days.
8. utilizing the method for white rot fungus degrading Nitenpyram as described in claim 1, which is characterized in that the step (3)
In, cultivation temperature is 30 DEG C, and incubation time is 5 days.
9. utilizing the method for white rot fungus degrading Nitenpyram as described in claim 1, which is characterized in that the step (3)
In, the concentration of Nitenpyram mother liquor is 0.1mM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810999741.3A CN109022295B (en) | 2018-08-30 | 2018-08-30 | Method for degrading nitenpyram by using white rot fungi |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810999741.3A CN109022295B (en) | 2018-08-30 | 2018-08-30 | Method for degrading nitenpyram by using white rot fungi |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109022295A true CN109022295A (en) | 2018-12-18 |
| CN109022295B CN109022295B (en) | 2021-09-17 |
Family
ID=64626262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810999741.3A Active CN109022295B (en) | 2018-08-30 | 2018-08-30 | Method for degrading nitenpyram by using white rot fungi |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109022295B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114634954A (en) * | 2022-02-18 | 2022-06-17 | 广州大学 | Method for biodegradation and conversion of organic pollutants |
| CN114699705A (en) * | 2022-05-06 | 2022-07-05 | 广州大学 | Method for degrading imidaclothiz by adopting white rot fungi |
| CN114703071A (en) * | 2022-05-06 | 2022-07-05 | 广州大学 | Method for degrading imidacloprid by adopting white rot fungi |
| CN114703240A (en) * | 2022-05-06 | 2022-07-05 | 广州大学 | Method for biologically synthesizing 5-hydroxy imidacloprid and olefin imidacloprid |
| CN115944879A (en) * | 2023-01-16 | 2023-04-11 | 广州大学 | Method for degrading polycyclic aromatic hydrocarbon pyrene and benzo [ a ] pyrene by using white rot fungi |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000152786A (en) * | 1998-09-18 | 2000-06-06 | Mercian Corp | Manganese peroxidase gene |
| JP2001245652A (en) * | 2000-03-03 | 2001-09-11 | Mercian Corp | Method for decomposing dioxin with manganese peroxidase and agent for decomposition treatment |
| JP2001314526A (en) * | 2000-03-03 | 2001-11-13 | Mercian Corp | Dioxin decomposing method and dioxin treatment agent by composite microorganism system |
| CN1533389A (en) * | 2001-07-27 | 2004-09-29 | Azole derivatives useful as insecticide | |
| JP2008263862A (en) * | 2007-04-20 | 2008-11-06 | National Agriculture & Food Research Organization | Method for creating transformant of white-rot fungus and transformant obtained by the method |
| CN101935683A (en) * | 2010-08-10 | 2011-01-05 | 中国科学院南京土壤研究所 | Method for measuring polyaromatic hydrocarbon degrading capability of white rot fungi |
-
2018
- 2018-08-30 CN CN201810999741.3A patent/CN109022295B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000152786A (en) * | 1998-09-18 | 2000-06-06 | Mercian Corp | Manganese peroxidase gene |
| JP2001245652A (en) * | 2000-03-03 | 2001-09-11 | Mercian Corp | Method for decomposing dioxin with manganese peroxidase and agent for decomposition treatment |
| JP2001314526A (en) * | 2000-03-03 | 2001-11-13 | Mercian Corp | Dioxin decomposing method and dioxin treatment agent by composite microorganism system |
| CN1533389A (en) * | 2001-07-27 | 2004-09-29 | Azole derivatives useful as insecticide | |
| JP2008263862A (en) * | 2007-04-20 | 2008-11-06 | National Agriculture & Food Research Organization | Method for creating transformant of white-rot fungus and transformant obtained by the method |
| CN101935683A (en) * | 2010-08-10 | 2011-01-05 | 中国科学院南京土壤研究所 | Method for measuring polyaromatic hydrocarbon degrading capability of white rot fungi |
Non-Patent Citations (2)
| Title |
|---|
| JIANQIAO WANG ET AL.: "Biotransformation of acetamiprid by the white-rot fungus Phanerochaete sordida YK-624", 《APPL MICROBIOL BIOTECHNOL》 * |
| TOSHIO MORI ET AL.: "Bioremediation of the neonicotinoid insecticide clothianidin by the white-rot fungus Phanerochaete sordida", 《JOURNAL OF HAZARDOUS MATERIALS》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114634954A (en) * | 2022-02-18 | 2022-06-17 | 广州大学 | Method for biodegradation and conversion of organic pollutants |
| CN114634954B (en) * | 2022-02-18 | 2023-08-08 | 广州大学 | Method for biodegradation and conversion of organic pollutants |
| CN114699705A (en) * | 2022-05-06 | 2022-07-05 | 广州大学 | Method for degrading imidaclothiz by adopting white rot fungi |
| CN114703071A (en) * | 2022-05-06 | 2022-07-05 | 广州大学 | Method for degrading imidacloprid by adopting white rot fungi |
| CN114703240A (en) * | 2022-05-06 | 2022-07-05 | 广州大学 | Method for biologically synthesizing 5-hydroxy imidacloprid and olefin imidacloprid |
| CN114699705B (en) * | 2022-05-06 | 2022-12-16 | 广州大学 | Method for degrading imidaclothiz by adopting white rot fungi |
| CN114703071B (en) * | 2022-05-06 | 2023-06-16 | 广州大学 | Method for degrading imidacloprid by using white rot fungi |
| CN114703240B (en) * | 2022-05-06 | 2023-10-13 | 广州大学 | Method for biosynthesis of 5-hydroxy imidacloprid and olefin imidacloprid |
| CN115944879A (en) * | 2023-01-16 | 2023-04-11 | 广州大学 | Method for degrading polycyclic aromatic hydrocarbon pyrene and benzo [ a ] pyrene by using white rot fungi |
| CN115944879B (en) * | 2023-01-16 | 2024-03-29 | 广州大学 | Method for degrading polycyclic aromatic hydrocarbon pyrene and benzo [ a ] pyrene by using white rot fungi |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109022295B (en) | 2021-09-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109022295A (en) | A method of utilizing white rot fungus degrading Nitenpyram | |
| Huang et al. | Microbial degradation of pesticide residues and an emphasis on the degradation of cypermethrin and 3-phenoxy benzoic acid: a review | |
| Yang et al. | Biodegradation of carbofuran by Pichia anomala strain HQ-C-01 and its application for bioremediation of contaminated soils | |
| Kulshrestha et al. | Fungal degradation of chlorpyrifos by Acremonium sp. strain (GFRC-1) isolated from a laboratory-enriched red agricultural soil | |
| Zhang et al. | Cometabolic biotransformation of fenpropathrin by Clostridium species strain ZP3 | |
| Chen et al. | Bioremediation of β-cypermethrin and 3-phenoxybenzaldehyde contaminated soils using Streptomyces aureus HP-S-01 | |
| CN103478147B (en) | Granule of Burkholderia vietnamiensis P418 nematicidal active substances and preparation thereof | |
| Cycoń et al. | Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil | |
| Xia et al. | Biodegradation of the herbicide 2, 4-dichlorophenoxyacetic acid by a new isolated strain of Achromobacter sp. LZ35 | |
| Shetty et al. | Biodegradation of cyclodiene insecticide endosulfan by Mucor thermohyalospora MTCC 1384 | |
| Birolli et al. | Biodegradation of the pyrethroid pesticide esfenvalerate by marine-derived fungi | |
| CN106754498A (en) | A kind of A Shi bacillus and its microbial inoculum and preparation method and application | |
| CN103497980B (en) | The preparation method of polyetherin A | |
| Yu et al. | Isolation and characterization of a Sphingomonas sp. strain F-7 degrading fenvalerate and its use in bioremediation of contaminated soil | |
| Wang et al. | Diazinon degradation by a novel strain Ralstonia sp. DI-3 and X-ray crystal structure determination of the metabolite of diazinon | |
| Math et al. | Isolation of a novel gene encoding a 3, 5, 6-trichloro-2-pyridinol degrading enzyme from a cow rumen metagenomic library | |
| Shi et al. | Chlorothalonil degradation by Ochrobactrum lupini strain TP-D1 and identification of its metabolites | |
| Birolli et al. | New role for a commercially available bioinsecticide: Bacillus thuringiensis Berliner biodegrades the pyrethroid cypermethrin | |
| do Nascimento Silva et al. | Novel combination of a biosurfactant with entomopathogenic fungi enhances efficacy against Bemisia whitefly | |
| LIU et al. | Imazamox microbial degradation by common clinical bacteria: Acinetobacter baumannii IB5 isolated from black soil in China shows high potency | |
| Ramya et al. | Biodegradation of synthetic pyrethroid pesticides under saline conditions by a novel halotolerant Enterobacter ludwigii | |
| Wu et al. | New roles for Bacillus thuringiensis in the removal of environmental pollutants | |
| Soltani Dashtbozorg et al. | Rhamnolipid adsorption in soil: factors, unique features, and considerations for use as green antizoosporic agents | |
| CN105198548A (en) | Bio-organic fertilizer with acetochlor degradation function and preparation method and application thereof | |
| CN103571769B (en) | Stenotrophomonas and application of Stenotrophomonas in degradation of acetamiprid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |