CN109020637A - A method of organic fertilizer is produced using stalk - Google Patents
A method of organic fertilizer is produced using stalk Download PDFInfo
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- CN109020637A CN109020637A CN201810875023.5A CN201810875023A CN109020637A CN 109020637 A CN109020637 A CN 109020637A CN 201810875023 A CN201810875023 A CN 201810875023A CN 109020637 A CN109020637 A CN 109020637A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a kind of methods using stalk production organic fertilizer, belong to field of biotechnology.The described method includes: in open environment, the strain W HP1 of the oyster cap fungus prepared is inoculated in stalk and carries out solid fermentation, the temperature of the solid fermentation is 25~36 DEG C, and the humidity of the solid fermentation is 50~90%, and fermentation obtained organic fertilizer after 15~20 days.The present invention carries out solid fermentation processing to a large amount of stalk in open environment by the strain W HP1 using oyster cap fungus, is organic fertilizer by stover, realizes efficiently fully utilizing to stalk, simultaneously, moreover it is possible to harvest the oyster cap fungus to grow fine.
Description
Technical field
The present invention relates to field of biotechnology, in particular to a kind of method using stalk production organic fertilizer.
Background technique
Stalk is often referred to wheat, corn, rice, potato, rape, cotton, sugarcane and other crops after harvesting seed
Remainder.
China is large agricultural country, and the yield of annual stalk is up to 900,000,000 tons, accounts for the 50% of biomass resource total amount.Straw at present
Stalk is usually used in manufacturing compost, and compost is utilized based on crop stalk, weeds, leaf, peat, rubbish and other wastes etc.
Raw material is wanted, people and animals' fecaluria is mixed through composting decomposition and organic fertilizer is made.
But existing stalk processing mode is only capable of 30% using China's stalk total output, remaining a large amount of stalk quilts
Discarded or burning, result in waste of resources simultaneously air pollution.Therefore, needing a kind of effective method can be improved the utilization of stalk
Rate.
Summary of the invention
It can be improved the utilization rate of stalk, the present invention to solve the problems, such as to need a kind of effective method in the prior art
Embodiment provides a kind of method using stalk production organic fertilizer.The technical solution is as follows:
In open environment, the strain W HP1 of the oyster cap fungus prepared is inoculated in stalk and under penetrating environment
Solid fermentation is carried out, the temperature of the solid fermentation is 25~36 DEG C, and the humidity of the solid fermentation is 50~90%, the straw
Stalk length is not more than 10cm, obtains organic fertilizer, wherein the strain W HP1 of the oyster cap fungus is in being preserved on July 4th, 2018
State's Type Tissue Collection, preservation address are China, Wuhan, Wuhan University, and deposit number is CCTCC NO:M
2018447。
Specifically, the time of the solid fermentation is 15~20 days.
Specifically, the stalk is used to be inoculated with by crushing, after boiling water infiltration and draining, the grain of the smashed stalk
Diameter is less than or equal to 10cm, and the mass ratio of the boiling water and the stalk is 1:(3~9), the infiltrating time is 12~36h,
The water content of the stalk after draining is 60~90%.
Specifically, the solid fermentation carries out in open fermenting case, and the height of the stalk is in the fermenting case
40~120cm offers multiple first ventilating heat dissipating holes on the fermenting case.
Specifically, in the solid fermentation, the strqw cover of heat release hole is had in the open-mouth covering of the fermenting case.
Specifically, it is inserted in the stalk multiple since the solid fermentation between solid fermentation the 8th~10 day
Ventilation duct offers multiple second ventilating heat dissipating holes on the ventilation duct.
Specifically, the preparation method of the strain W HP1 of the oyster cap fungus includes: by the bacterium cultivated and have oyster cap fungus
In the plate access level-one kind fluid nutrient medium of kind WHP1, and at a temperature of 25~36 DEG C, 50~250rpm shaken cultivation 5~
9 days, level liquid strain is obtained, the level liquid strain is seeded to the training of level-one kind liquid according to 2~10% inoculum concentration
It supports in base, and at a temperature of 25~36 DEG C, 50~250rpm shaken cultivation 3~6 days, secondary liquid strain is obtained, by stalk
It is mixed after crushing with water, after sterilization treatment, obtains tray solid matrix culture medium, the secondary liquid strain is inoculated in
It on the tray solid matrix culture medium, and is cultivated 10~15 days at a temperature of 25~36 DEG C, obtains the oyster cap fungus
Strain W HP1, the strain W HP1 of the oyster cap fungus and the mass ratio of the stalk are 1:(5~20).
Specifically, the level-one kind fluid nutrient medium and the second level kind fluid nutrient medium are potato Liquid Culture
Base.
Specifically, the stalk is corn stover, wheat stalk or beans stalk.
Specifically, make the water content of the organic fertilizer less than 20% by drying process the obtained organic fertilizer, it will
The organic fertilizer after drying is packed after crushing, screening and granulation.
Technical solution provided in an embodiment of the present invention has the benefit that the embodiment of the invention provides a kind of utilizations
The method that stalk produces organic fertilizer, this method can by solid fermentation in open environment using the strain W HP1 of oyster cap fungus
Using a large amount of stalk as solid fermentation matrix, in solid fermentation process, the strain W HP1 of the oyster cap fungus can be efficiently used
The kinds of ingredients of lignocellulosic in stalk, lignin especially difficult to degrade, so that the organic principle in stalk is more conducive to quilt
Crop is absorbed and utilized, by oyster cap fungus to the solid state fermentation of stalk, so that the stalk after solid fermentation can be used as organic fertilizer, it should
The content of total nutrient and total humic acid in organic fertilizer is higher, can improve the physicochemical property of soil, promotes the growth of plant, right
There is great function in the fertility for promoting soil.Simultaneously, moreover it is possible to harvest the oyster cap fungus to grow fine.It is thus achieved that a large amount of
Stalk efficiently fully utilizes.
The strain W HP1 of the oyster cap fungus is preserved in China typical culture collection center on July 4th, 2018, preservation
Location is China, Wuhan, Wuhan University, and Latin literary fame is known as Pleurotus ostreatus WHP1, deposit number CCTCC
NO:M 2018447。
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing.
Fig. 1 is the photo of the oyster cap fungus provided in an embodiment of the present invention grown in stalk；
Fig. 2 is the overlooking structure diagram of fermenting case provided in an embodiment of the present invention；
Fig. 3 is the side structure schematic view of fermenting case provided in an embodiment of the present invention.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention will be made into one below
Step ground detailed description.
The embodiment of the present invention provides a kind of method using stalk production organic fertilizer, this method comprises: in open environment,
The strain W HP1 of the oyster cap fungus prepared is inoculated in stalk and carries out solid fermentation, solid fermentation under penetrating environment
Temperature be 25~36 DEG C, the humidity of solid fermentation is 50~90%, and stalk obtains organic fertilizer after solid fermentation.It is realizing
When, stalk can be uniformly mixed with strain by blender.In the present embodiment, open environment refers to the environment of non-sterilizing, tool
Body, which is for the environment of closing and sterilizing in laboratory.Penetrating environment refers to as training
The stalk for supporting base has the function of breathing freely, and when realizing, air hole mode can be arranged in stalk, naturally flowed using air
It is dynamic to reach penetrating requirement.
Specifically, the time of solid fermentation is 15~20 days.Under the above conditions, the strain W HP1 of oyster cap fungus can be
It can grow within 15~20 days to the weight of single oyster cap fungus between 0.8~2 kilogram, specific oyster cap fungus growing state is shown in Fig. 1.
Specifically, stalk by crushing, after boiling water infiltration and draining for being inoculated with, the partial size of smashed stalk be less than or
Equal to 10cm, the mass ratio of boiling water and stalk can be 1:(3~9), infiltrating time can be 12~36h, the stalk after draining
Water content can be 60~90%.By boiling water infiltration simple exterminating bacterium can be carried out for stalk.It, can also when realizing
To be infiltrated using cold water, steam sterilizing is carried out to stalk again after draining or heat water-spraying sterilizes.
Specifically, solid fermentation carries out in open fermenting case, and the height of stalk is 40~120cm, hair in fermenting case
Multiple first ventilating heat dissipating holes 8 are offered on ferment case.(this is highly height material bed in fermenting case to the height of stalk in fermenting case
Degree, the material bed stalk referred to by crushing) it can be 45~125cm.When realizing, every 10cm on fermenting case2It can divide
4~5 the first ventilating heat dissipating holes 8 of cloth, the aperture of each first ventilating heat dissipating hole 8 is can be with 7~9mm.First ventilation and heat
Hole 8 is used to be the stalk ventilation and heat in fermenting case, and supplies oxygen for oyster cap fungus.
Fig. 2 and Fig. 3 is the structural schematic diagram of fermenting case provided in an embodiment of the present invention.As shown in Figures 2 and 3, it ferments
Case includes main body, and main body includes: that the first side plate 1, the second side plate 2, third side plate 3, the 4th side plate 4, the ventilation of bottom plate 5, first dissipate
Heat pipe 6 and the second ventilation and heat pipe 7.First side plate 1, the second side plate 2, third side plate 3 and the 4th side plate 4 are vertically mounted on bottom
It the one side of plate 5 and is sequentially connected and surrounds frame structure, the first side plate 1 and third side plate 3 align, the first side plate 1, second
Side plate 2, third side plate 3, the 4th side plate 4 and bottom plate 5 limit an accommodation space.First side plate 1, the second side plate 2, third side plate
3, multiple first ventilating heat dissipating holes 8 are offered on the 4th side plate 4 and bottom plate 5.As shown in Fig. 2, being also provided with first on bottom plate 5
Ventilation and heat pipe installation through-hole 10, the bottom end of the first ventilation and heat pipe 6 are inserted into the first ventilation and heat pipe installation through-hole 10, the
Accommodation space is stretched out on the top of one ventilation and heat pipe 6, offers the second ventilation and heat respectively on the first side plate 1 and third side plate 3
Pipe installation through-hole 11, one end of the second ventilation and heat pipe 7 are inserted into the second ventilation and heat pipe installation through-hole 11 of the first side plate 1
Interior, the other end of the second ventilation and heat pipe 7 is inserted into the second ventilation and heat pipe installation through-hole 11 of third side plate 3.First is logical
Multiple second ventilating heat dissipating holes 9 are offered on wind heat-dissipating pipe 6 and the second ventilation and heat pipe 7.
Further, in solid fermentation, the strqw cover 13 of heat release hole 14 is had in the open-mouth covering of fermenting case.It is realizing
When, strqw cover 13 and 5 face of bottom plate are arranged.Strqw cover 13 can use plastics roller shutter, and strqw cover 13 can prevent moisture in fermenting case
Excessive evaporation, to guarantee that the solid fermentation in fermenting case can be normally carried out.
Further, multiple heat release holes 14 can be set on strqw cover 13.Every 5cm23~5 heat release holes can be distributed
14, the aperture of each heat release hole 14 all can be 2~4cm, multiple heat release holes 14 can be convenient for the heat dissipation inside fermenting case.
Specifically, fermenting case can also include that the first ventilation and heat pipe fixing piece 17 and fixed ring 17a, fixed ring 17a cover
It is located on the first ventilation and heat pipe fixing piece 17, the first ventilation and heat pipe fixing piece 17 is by fixed ring 17a and the first side plate 1 and
The connection of three side plates 3.
Further, the top of the first side plate 1 and third side plate 3 offers card slot 18, the first ventilation and heat Guan Gu respectively
Determining part 17 includes two fixed links, and one end difference fixed ring of two fixed links connects, and the other end of two fixed links is pacified respectively
In the card slot 18 of the first side plate 1 and the card slot 18 of third side plate 3.Card slot 18 is easy to process, can be logical with simple realization first
The fixation of wind heat-dissipating pipe fixing piece 17.
Specifically, multiple ventilation ducts are inserted in stalk between solid fermentation the 8th~10 day since solid fermentation,
Ventilation duct may include the first ventilation and heat pipe 6 and the second ventilation and heat pipe 7, the first ventilation and heat pipe 6 and the second ventilation and heat
Multiple second ventilating heat dissipating holes 9 are offered on pipe 7.When realizing, suitable size can be selected according to the size of fermenting case
Ventilation duct, multiple ventilation duct arranged for interval.In the present embodiment, the diameter of each ventilation duct all can be 7~9cm, Mei Ge
The aperture of two ventilating heat dissipating holes 9 all can be 2~3mm.In solid fermentation early period, oyster cap fungus can discharge greatly during the growth process
The heat of amount, a large amount of heat can be gathered in inside stalk, and ventilation duct can supply oxygen for stalk inner ventilation, be prevented inside stalk
Temperature is excessively high, to be conducive to the growth of oyster cap fungus, oyster cap fungus is avoided to influence growth because temperature is excessively high.
Specifically, the preparation method of the strain W HP1 of oyster cap fungus includes: the bacterium for the oyster cap fungus for having gesture excellent culture
The plate of kind WHP1 is forwarded in access level-one kind fluid nutrient medium using punch, and at a temperature of 25~36 DEG C, 50~
250rpm shaken cultivation 5~9 days, level liquid strain is obtained, by level liquid strain according to the inoculum concentration of 2~10% (V/V)
It is seeded in second level kind fluid nutrient medium, and at a temperature of 25~36 DEG C, 50~250rpm shaken cultivation 3~6 days, obtains two
Grade liquid spawn, it is 20~80 mesh that stalk, which is crushed to granularity, stalk and water is stirred evenly, and the weight ratio of stalk and water is
The mixture of every 100g stalk and water is dispensed into tray by 1:2.5~5, and tray is wrapped up by the first sealing film, is used in combination
Rubber band is fixed, and the tray wrapped is covered by the second sealing film, and is fixed with rubber band.By tray in 121 DEG C of items
Sterilize 1h under part, obtains tray solid matrix culture medium.Remove the first sealing film and the second sealing film, is existed using inoculated tube
Made a call on tray culture medium 10~20 depth be 1~2cm, the hole that diameter is 0.5~2cm, 0.5~2mL second level liquid is injected in every hole
Tray solid matrix culture medium after inoculation is wrapped up by third sealing film, and is fixed with rubber band by body strain, is placed in
Cultivated 10~15 days in 25~36 DEG C of incubator, obtain the strain W HP1 of oyster cap fungus, the strain W HP1 of the oyster cap fungus with
The mass ratio of stalk is 1:(5~20).When realizing, using the excellent oyster cap fungus of slant activation growing way strain W HP1 it is flat
Plate is forwarded in the first fluid nutrient medium using punch, and the aperture of punch can be 6~12mm, and inoculum concentration can be every
3~10 holes are accessed in the first fluid nutrient medium of 100mL, in 50~250rpm shaken cultivation 5~9 days, to select at 25~36 DEG C
Biomass is more, mycelia bulb diameter is smaller and has the level liquid strain of obvious prickle for being inoculated with.
Specifically, level-one kind fluid nutrient medium and second level kind fluid nutrient medium all can be potato fluid nutrient mediums.
Wherein, potato fluid nutrient medium the preparation method is as follows: by potato decontamination, clean and remove the peel, accurately weigh
200g peeled potatoes, and be sliced and be put into saucepan, the distilled water that 1.5L or so is added into pot heats simultaneously together with potato
It boils, slightly boiled 30min is kept after boiling, potato leachate is obtained by eight layers of filtered through gauze, by potato leachate constant volume
To 1L, glucose 20g is then added and is dispensed, is dispensed with every bottle of 100mL into the triangular flask of 250mL, is gone out by 115 DEG C
It is spare after bacterium 20min.
Specifically, stalk is corn stover, wheat stalk or beans stalk.
Specifically, make the water content of organic fertilizer less than 20%, after drying by being dried obtained organic fertilizer
Organic fertilizer is packed and stored after crushing and screening and being granulated.It can be conducive to the storage and transport of organic fertilizer in this way.
Embodiment one
The embodiment of the invention provides a kind of method using stalk production organic fertilizer, stalk uses corn stover, fermentation
The height of corn stover is 40cm in case, and boiling water is added into corn stover in 3~8cm for the partial size of smashed corn stover
Infiltrate 36h, wherein the mass ratio of boiling water and stalk is 1:3, extra moisture is filtered out after infiltration, and make the aqueous of corn stover
Amount is 80%, and the corn stover prepared is deposited in fermenting case, and the height of fermenting case is 60cm；By it is above-mentioned prepare it is rough
The solid matrix strain for the strain W HP1 that skin is picked up the ears is uniformly mixed with the agitated machine of corn stover, wherein the strain of oyster cap fungus
WHP1 is accessed in corn stover according to the inoculum concentration of mass ratio 1:5 (W/W), and is put into progress solid fermentation training in aerlbic culture case
It supports, the temperature of solid fermentation is 25 DEG C, the humidity 70% of solid fermentation, and the time of solid state fermentation is 15~20 days, is sent out in solid
When ferment, the strqw cover 13 with heat release hole 14 can be covered in the open-mouth of fermenting case, to solid fermentation the 8th since solid fermentation
Between~10 days, multiple first ventilation and heat pipes 6 can be inserted in stalk, multiple second ventilation and heats are inserted on fermenting case
Pipe 7, obtains organic fertilizer, by organic fertilizer after drying to moisture is less than 20%, then is crushed, is sieved, detected through sieve
It is put in storage with after packaging.After fermentation, the available oyster cap fungus to grow fine, oyster cap fungus as shown in Figure 1, the rough skin
It picks up the ears color white big, and tasty mouthfeel, the weight of single oyster cap fungus is between 0.8~2 kilogram.
Embodiment two
The embodiment of the invention provides a kind of method using stalk production organic fertilizer, stalk uses wheat stalk, fermentation
The height of wheat stalk is 60cm in case, and boiling water is added into wheat stalk in 3~8cm for the partial size of smashed wheat stalk
Infiltration is for 24 hours, wherein the mass ratio of boiling water and stalk is 1:9, extra moisture is filtered out after infiltration, and make the aqueous of wheat stalk
Amount is 60%, and the wheat stalk prepared is deposited in fermenting case, and the height of fermenting case is 90cm；By it is above-mentioned prepare it is rough
The strain W HP1 that skin is picked up the ears is uniformly mixed with the agitated machine of wheat stalk, wherein the strain W HP1 of oyster cap fungus is according to mass ratio
In the inoculum concentration access wheat stalk of 1:15 (W/W), and it is put into progress solid fermentation culture in aerlbic culture case, solid fermentation
Temperature is 36 DEG C, the humidity 50% of solid fermentation, and the time of solid state fermentation is 15~20 days, in solid fermentation, in fermenting case
Open-mouth can cover the strqw cover 13 with heat release hole 14, between solid fermentation the 8th~10 day since solid fermentation,
Multiple first ventilation and heat pipes 6 can be inserted in stalk, and multiple second ventilation and heat pipes 7 are inserted on fermenting case, are obtained organic
Fertilizer, by organic fertilizer through it is dry to moisture less than 20% after, then crushed, sieved, detected and packed through sieve after be put in storage.
After fermentation, the available oyster cap fungus to grow fine, it is the oyster cap fungus color white big, and tasty mouthfeel, single rough skin
The weight picked up the ears is between 0.8~2 kilogram.
Embodiment three
The embodiment of the invention provides a kind of method using stalk production organic fertilizer, stalk uses wheat stalk, fermentation
The height of wheat stalk is 120cm in case, and boiling water is added into wheat stalk in 3~8cm for the partial size of smashed wheat stalk
Infiltrate 12h, wherein the mass ratio of boiling water and stalk is 1:4, extra moisture is filtered out after infiltration, and make the aqueous of wheat stalk
Amount is 90%, and the wheat stalk prepared is deposited in fermenting case, and the height of fermenting case is 125cm；
The secondary liquid strain of the strain W HP1 of the above-mentioned oyster cap fungus prepared is mixed with the agitated machine of wheat stalk
Uniformly, wherein the strain W HP1 of oyster cap fungus is accessed in wheat stalk according to the inoculum concentration of volume mass ratio 1:20 (V/W), and
It is put into progress solid fermentation culture in aerlbic culture case, the temperature of solid fermentation is 37 DEG C, the humidity 90% of solid fermentation, solid-state
The time of fermentation is 15~20 days, in solid fermentation, can cover the strqw cover with heat release hole 14 in the open-mouth of fermenting case
13, between solid fermentation the 8th~10 day since solid fermentation, multiple first ventilation and heat pipes 6 can be inserted in stalk,
Multiple second ventilation and heat pipes 7 are inserted on fermenting case, obtain organic fertilizer, by organic fertilizer after drying to moisture is less than 20%,
Crushed again, sieved, detected and packed through sieve after be put in storage.After fermentation, it while available growing fine
Oyster cap fungus, it is the oyster cap fungus color white big, and tasty mouthfeel, the weight of single oyster cap fungus is between 0.8~2 kilogram.
Organic fertilizer prepared by embodiment one to embodiment three is subjected to Components identification respectively, concrete outcome is as follows:
Embodiment one provide organic fertilizer compared to unleavened stalk after solid fermentation 15 days, total nutrient content
4.21% is risen to by 3.78%, total humic acid rises to 29.4% by 27.7%.
The organic fertilizer that embodiment two and embodiment three provide compared to unleavened stalk after solid fermentation 20 days,
Total nutrient rises to 3.23% by 3.15%, and total humic acid rises to 29.82% by 26.88%, after solid fermentation 25 days, always
Nutrient is further up to 3.85%, and total humic acid rises to 31.77%.After solid fermentation 20 days and 25 days, straw substrate
In content of lignin drop to 17.48% and 16.12% by 21.26%, show that whiterot fungi is effectively abolished to a certain extent
Lignin barrier in lignocellulosic, is conducive to the further release of nutriment in straw substrate.
The embodiment of the invention provides a kind of methods using stalk production organic fertilizer.This method utilizes the bacterium of oyster cap fungus
Kind WHP1 can be using a large amount of stalk as solid fermentation matrix, in solid fermentation process by solid fermentation in open environment
In, the strain W HP1 of the oyster cap fungus can efficiently use the kinds of ingredients of lignocellulosic in stalk, wood especially difficult to degrade
Quality, so that the organic principle in stalk is more conducive to be absorbed by crops utilization, by the oyster cap fungus to the solid state fermentation of stalk,
So that the stalk after solid fermentation can be used as organic fertilizer, the content of total nutrient and total humic acid in the organic fertilizer is higher, can be with
The physicochemical property for improveing soil, promotes the growth of plant, and the fertility for promoting soil has great function.Simultaneously, moreover it is possible to receive
Obtain the oyster cap fungus to grow fine.It is thus achieved that efficiently fully utilizing to a large amount of stalks.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of method using stalk production organic fertilizer, which is characterized in that the described method includes:
In open environment, the strain W HP1 of the oyster cap fungus prepared is inoculated in stalk and is carried out under penetrating environment
Solid fermentation, the temperature of the solid fermentation are 25~36 DEG C, and the humidity of the solid fermentation is 50~90%, are sent out by solid
Organic fertilizer is obtained after ferment, wherein the strain W HP1 of the oyster cap fungus is preserved in Chinese Typical Representative culture on July 4th, 2018
Collection, preservation address are China, Wuhan, Wuhan University, and deposit number is CCTCC NO:M 2018447.
2. the method according to claim 1, wherein the time of the solid fermentation is 15~20 days.
3. the method according to claim 1, wherein being used after the stalk is by crushing, boiling water infiltrates and draining
In inoculation, the partial size of the smashed stalk is less than or equal to 10cm, and the mass ratio of the boiling water and the stalk is 1:(3
~9), the infiltrating time is 12~36h, and the water content of the stalk after draining is 60~90%.
4. according to the method described in claim 3, it is characterized in that, the solid fermentation carries out in open fermenting case, institute
The height for stating the stalk in fermenting case is 40~120cm, offers multiple first ventilating heat dissipating holes on the fermenting case.
5. according to the method described in claim 4, it is characterized in that, in the solid fermentation, in the opening of the fermenting case
Place's covering has the strqw cover of heat release hole.
6. the method according to claim 1, wherein to solid fermentation the 8th~10 since the solid fermentation
Between it, multiple ventilation ducts are inserted in the stalk, and multiple second ventilating heat dissipating holes are offered on the ventilation duct.
7. the method according to claim 1, wherein the preparation method packet of the strain W HP1 of the oyster cap fungus
It includes: being cultivated described in the plate access level-one kind fluid nutrient medium for having the strain W HP1 of oyster cap fungus, and in 25~36 DEG C
At a temperature of, 50~250rpm shaken cultivation 5~9 days, obtain level liquid strain, by the level liquid strain according to 2~
10% inoculum concentration is seeded in level-one kind fluid nutrient medium, and at a temperature of 25~36 DEG C, 50~250rpm shaken cultivation 3
~6 days, secondary liquid strain is obtained, is mixed after stalk is crushed with water, after sterilization treatment, obtains the training of tray solid matrix
Base is supported, the secondary liquid strain is inoculated on the tray solid matrix culture medium, and is trained at a temperature of 25~36 DEG C
It supports 10~15 days, obtains the strain W HP1 of the oyster cap fungus, the strain W HP1 of the oyster cap fungus and the quality of the stalk
Than for 1:(5~20).
8. the method according to claim 1, wherein the level-one kind fluid nutrient medium and the second level kind liquid
Culture medium is potato fluid nutrient medium.
9. the method according to claim 1, wherein the stalk is corn stover, wheat stalk or beans straw
Stalk.
10. the method according to claim 1, wherein the obtained organic fertilizer is made institute by being dried
The water content of organic fertilizer is stated less than 20%, the organic fertilizer after drying is packed after crushing, screening and granulation.
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| CN201810875023.5A CN109020637A (en) | 2018-08-03 | 2018-08-03 | A method of organic fertilizer is produced using stalk |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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