CN109007402A - Feed addictive of layer chicken and preparation method thereof - Google Patents
Feed addictive of layer chicken and preparation method thereof Download PDFInfo
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- CN109007402A CN109007402A CN201810629988.6A CN201810629988A CN109007402A CN 109007402 A CN109007402 A CN 109007402A CN 201810629988 A CN201810629988 A CN 201810629988A CN 109007402 A CN109007402 A CN 109007402A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 244000241838 Lycium barbarum Species 0.000 claims abstract description 15
- 235000015459 Lycium barbarum Nutrition 0.000 claims abstract description 15
- 235000015468 Lycium chinense Nutrition 0.000 claims abstract description 15
- 235000003434 Sesamum indicum Nutrition 0.000 claims abstract description 15
- 240000008042 Zea mays Species 0.000 claims abstract description 14
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 14
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- 238000000855 fermentation Methods 0.000 claims abstract description 13
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- 244000000231 Sesamum indicum Species 0.000 claims abstract 5
- 239000000843 powder Substances 0.000 claims description 24
- 244000005700 microbiome Species 0.000 claims description 10
- 244000063299 Bacillus subtilis Species 0.000 claims description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 6
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 6
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 6
- 235000017784 Mespilus germanica Nutrition 0.000 claims description 6
- 244000182216 Mimusops elengi Species 0.000 claims description 6
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- 238000007254 oxidation reaction Methods 0.000 abstract description 2
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- 239000002609 medium Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 241000605059 Bacteroidetes Species 0.000 description 3
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- 241000192125 Firmicutes Species 0.000 description 3
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- 238000010521 absorption reaction Methods 0.000 description 3
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- 239000000835 fiber Substances 0.000 description 3
- 229930003935 flavonoid Natural products 0.000 description 3
- 150000002215 flavonoids Chemical class 0.000 description 3
- 235000017173 flavonoids Nutrition 0.000 description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 description 3
- 235000013824 polyphenols Nutrition 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 241000186046 Actinomyces Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000606125 Bacteroides Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000566145 Otus Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000011054 acetic acid Nutrition 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
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- 235000015278 beef Nutrition 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
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- 230000000968 intestinal effect Effects 0.000 description 2
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- 241001453172 Fusobacteria Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
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- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
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- 230000036541 health Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
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- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
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- 235000009566 rice Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Botany (AREA)
- Birds (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of feed addictive of layer chicken and preparation method thereof.The feed addictive of layer chicken is made of the raw material of following parts by weight: wolfberry leaf, fructus lycii handle, 15~25 parts of microbial fermentation product of corn flour and sesame seed meal, 70~80 parts of mealy potato, 2~6 parts of fructus lycii, 0.5~1 part, 0.1~0.3 part of thyme octagonal.And devise a kind of preparation method of feed addictive of layer chicken.Feed addictive of layer chicken of the invention can be improved the oxidation resistance of laying hen, and enhance the immunity of laying hen, be conducive to improve production performance and Egg Quality, preparation method is easy to operate, cost is controllable.
Description
Technical field
The present invention relates to poultry and livestock feed technical fields, and in particular to a kind of feed addictive of layer chicken and preparation method thereof.
Background technique
With the fast development of livestock and poultry breeding industry, animal and fowl fodder and feed additive industry are also expanded rapidly, and are gone out in the market
The various feeds and feed addictive for reducing raising livestock and poultry costs, improving immunity and production performance etc. in livestock and poultry are showed.However
Due to the aquaculture model of the growth traits of livestock and poultry directive breeding, the nutrition supply of optimization and precision, cause the digestion of livestock and poultry
Absorption frequency is in high-intensitive state, this serious livestock and poultry destroys the integrality of intestinal mucosa, damages its intestinal health, or even cause intestines
Tract disease.Intestinal flora imbalance, immunity reduction are also resulted in, finally livestock and poultry production performance is caused to decline.
Since there are high-density rearing's environment, frequently immune and later period physilogical characteristics etc. of laying eggs for the breeding layer chicken industry in China
Problem, China's performance in layers and quality of laying eggs cannot be guaranteed well.And China is every year because of eggshell quality problem
Caused by egg loss up to 10% or more, this severely limits the development of breeding layer chicken industry.So enhancing immunity of layer chicken, mentioning
High Egg Production of Laying Hens production performance and Egg Quality are current aquaculture technical problems urgently to be resolved.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of feed addictive of layer chicken and preparation method thereof, to promote
So that laying hen is grown up healthy and sound, is conducive to improve Layer Production Performance and Egg Quality.
In order to solve the above technical problems, the present invention adopts the following technical scheme:
A kind of feed addictive of layer chicken is designed, is made of the raw material of following parts by weight: wolfberry leaf, fructus lycii handle, corn flour and sesame
15~25 parts of the microbial fermentation product of the dregs of rice, 70~80 parts of mealy potato, 2~6 parts of fructus lycii, 0.5~1 part octagonal, thyme 0.1
~0.3 part.
Wherein, the microbial fermentation product the preparation method comprises the following steps:
Wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal are taken, is pulverized and sieved, microorganism is added；It is dried after culture, obtains tunning.
It is preferred: the wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal mass ratio be 0.5~1.5:0.5~1.5:
0.1~0.3:0. 05~0.2.
Preferred: the microorganism is lactobacillus plantarum BNCC187897 and/or bacillus subtilis AS1.9086.
Preferred: the mass ratio of the microorganism and fermentation liquid is 5:1.
Preferred: the wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal etc. crushed 50~70 meshes.
Design a kind of preparation method of feed addictive of layer chicken, comprising the following steps:
(1) wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal are taken, is pulverized and sieved, microorganism is added, dries, obtain after culture
Tunning；
(2) mealy potato is mixed to gained tunning and is uniformly mixed, then sequentially add the star aniseed powder, hundred
In face powder and medlar powder, stirring to be uniformly mixed to get.
Compared with prior art, the beneficial technical effect of the present invention lies in:
1. feed addictive of layer chicken of the present invention is the growth and development characteristics for laying hen and designs, each component is worked in coordination synergy,
Laying hen cecum flora structure can be substantially improved, can not only enhance fowl abilities of digestive and absorption, and then effectively improve Egg Production of Laying Hens
Can, and Egg Quality is improved, and the immunocompetence of laying hen can also be improved.
2. furthermore, prepared by the present invention to contain a large amount of crude protein, crude fibre, cinder in the tunning of addition
Divide, total amino acid, moisture, acetic acid, propionic acid, lactic acid, Thick many candies, polyphenol, crude flavonoid powder, wherein polyphenol is important bioactivity
Substance has strong anti-oxidation characteristics, can enhance livestock and poultry oxidation resistance；Crude flavonoid powder has very strong anti-oxidant, anti-toxin for liver
With it is anti-inflammatory and other effects, the immunity of livestock and poultry can be enhanced, promote the healthy growth of livestock and poultry, be conducive to improve livestock and poultry production performance and production
Egg Quality；Research finds octagonal and thyme plants essential oil rich in, has antibacterium, fungi, virus and anti parasitic
Etc. functions, substitute antibiotics can be played the role of in the present invention；Medlar powder added by the present invention can not only improve poultry
The immunocompetence of fowl can also enhance the fecundity of livestock and poultry, improve laying rate；In feed addictive of layer chicken of the invention rationally
Collocation crude protein, crude fibre and total amino acid, can enhance the abilities of digestive and absorption of livestock and poultry.
3. raw material sources of the present invention are extensive, each components matching synergy, lower production costs, significant effect are conducive to raiseeing
It is widely applied in fowl production.
Detailed description of the invention
Fig. 1 is the species relative abundance column diagram in door level, and wherein C is control group, and S is test group；
Fig. 2 is the species relative abundance column diagram belonged in level, and wherein C is control group, and S is test group；
Fig. 3 is the horizontal Egg Production of Laying Hens later period cecum flora principal component analysis figure of door, and abscissa indicates first principal component, percentage in figure
Than then indicating first principal component to the contribution margin of sample difference；Ordinate indicate Second principal component, percentage indicate second it is main at
Divide the contribution margin to sample difference；Each point in figure indicates that a sample, same group of sample use same color table
Show, similarly hereinafter；
Fig. 4 is to belong to horizontal Egg Production of Laying Hens later period cecum flora principal component analysis figure.
Specific embodiment
Illustrate a specific embodiment of the invention with reference to the accompanying drawings and examples, but following embodiment is used only in detail
It describes the bright present invention in detail, does not limit the scope of the invention in any way.
Related instrument and equipment is routine instrument device unless otherwise instructed in the examples below；It is related
Product is commercially available conventional products unless otherwise instructed；Related test method or analysis method, unless otherwise instructed,
For conventional method.
Embodiment one: feed addictive of layer chicken preparation
1. screening fermenting microbe
Through a large amount of experimental study, preferably go out to be suitable for the lactobacillus plantarum of wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal mixture
During BNCC187897 freeze-dried powder (being purchased from Chinese microorganism strain collection, BNCC), bacillus subtilis AS1.9086(are purchased from
Institute of microbiology of the academy of sciences of state Culture Collection Center) it is used as fermentative microorganism, tunning nutriment comprehensive and abundant can
The materials such as fully degraded wolfberry leaf, fructus lycii handle, sesame seed meal generate the effective component more suitable for fowl only.
2. culture medium
Nutrient broth medium (NB): powdered beef 3g, peptone 10g, sodium chloride 5g, distilled water 1000mL.PH value 6.8~7.0,
121 DEG C of sterilizing 15min.
Nutrient agar: on the basis of nutrient broth medium, 15 ~ 20g of agar powder is added in every liter of culture solution.
MRS broth bouillon: peptone 10g, yeast powder 4g, powdered beef 5g, glucose 20g, sodium acetate 5g, citric acid three
Ammonium 2g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.2g, manganese sulfate 0.05g, Tween 80 1mL.
MRS agar medium: on the basis of MRS broth bouillon, 15~20g of agar powder is added in every liter of culture solution.
3. prepared by seed liquor
Bacillus subtilis: the Bacillus subtilis strain streak inoculation that picking freezes on a small quantity to sterilized nutrient agar culture
On base, 37 DEG C of constant temperature incubations carry out actication of culture for 24 hours, and the strain of activation is then inoculated in sterilized nutrient broth medium
In, it is placed in constant-temperature table, 37 DEG C of temperature, revolving speed 180r/min, cultivates spare for 24 hours.
Lactobacillus plantarum: the lactobacillus plantarum strain streak inoculation that picking freezes on a small quantity to sterilized MRS agar culture
On base, 37 DEG C of constant temperature incubations carry out actication of culture for 24 hours, and the strain of activation is then inoculated in sterilized MRS broth bouillon
In, it is spare for 24 hours to be placed in 37 DEG C of cultures in constant incubator.
4. composition and the preparation of fermentation substrate
It takes wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal to crush, crosses 60 meshes.4 kinds of raw materials are mixed by 1 mass ratio of 1:1:0.2:0.
It closes, it is spare.
5. solid fermentation
Fermentation condition:
Take fermentation substrate, feed liquid by the mass ratio of 10:1 inoculum concentration addition, wherein lactobacillus plantarum and bacillus subtilis
Solid-liquid ratio is 5:1.
Than initial water content 35%, 37 DEG C are cultivated 72 hours quality, and fermented sample send Henan sea the auspicious positive limited public affairs of detection technique
Department's detection.
Conventional nutrients are as shown in table 1 in tunning:
Conventional nutrients (dry matter meter) in 1 tunning of table
| Crude protein % | Crude fibre % | Coarse ash % | Total amino acid % | |
| Content | 18.11 | 8.81 | 23.86 | 13.08 |
| Foundation | GB/T 6432-1994 | GB/T 6434-2006 | GB/T 6438-2007 | GB/T18246-2000 |
Bioactive ingredients are as shown in table 2 in tunning:
Bioactive ingredients in 2 tunning of table
| Acetic acid mg/kg | Propionic acid mg/kg | Lactic acid mg/kg | Thick many candies % | Polyphenol % | Crude flavonoid powder % | |
| Content | 2296.18 | 102.21 | 974.52 | 2.40 | 0.98 | 0.91 |
| Foundation | Gas-chromatography | Gas-chromatography | GB/T 23877-2009 | SN/T4260-2015 | Spectrophotometric | Spectrophotometric |
6. tunning is dried
50 DEG C of tunning drying, it is spare.
7. preparing feed addictive of layer chicken
Specifically includes the following steps:
3~5min is mixed to being uniformly mixed with 20 parts of tunnings in 75 parts of mealy potatos, then sequentially adds 0.8 part of illiciumverum
Powder, 0.2 part of thyme powder and 4 parts of medlar powders, stirring 5min to be uniformly mixed to get.
Embodiment two: feed addictive of layer chicken preparation
With embodiment one the difference is that:
It is mixed 1. wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal are crushed in fermentation substrate by 0.5:1.5:0.1:0.2 mass ratio,
It is spare.
2. preparing feed addictive of layer chicken
3~5min is mixed to being uniformly mixed with 15 parts of tunnings in 70 parts of mealy potatos, then sequentially adds 0.5 part of illiciumverum
Powder, 0.1 part of thyme powder and 2 parts of medlar powders, stirring 5min to be uniformly mixed to get.
Embodiment three: feed addictive of layer chicken preparation
With embodiment one the difference is that:
1. wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal crush mixed by 1.5:0.5:0.3:0.05 mass ratio in fermentation substrate
It closes, it is spare.
2. preparing feed addictive of layer chicken
3~5min is mixed to being uniformly mixed with 25 parts of tunnings in 80 parts of mealy potatos, then sequentially adds 1 part of illiciumverum
Powder, 0.3 part of thyme powder and 6 parts of medlar powders, stirring 5min to be uniformly mixed to get.
Example IV: influence research of the feed addictive of layer chicken to Egg Quality
1. experimental animal
Animal experiment is carried out in Beijing prosperous Sheng cultivation Co., Ltd, and layer breed is the blue brown laying hen in sea.
2. experimental design and management
Test uses single factor test completely randomized design, selects 406 ages in days sea similar in 5000 health, active, laying rate, weight
Blue brown laying hen is randomly divided into 2 processing groups, respectively control group and test group.If pre-feeding period 7 days, pre-feeding period was interior to extra large blue brown egg
Chicken prevented epidemic, feeding and drinking-water, then enters formal experimental period.Control group fed conventional base diet, test group feeding
The diet of feed addictive described in the 0.5% addition embodiment one in conventional base diet by its weight, experimental period are 49 days.
3. daily management
Test is raised using cage mode in giving up, natural lighting, all laying hen free waters and feeding, disinfection and it is immune routinely
Method carries out.Daily 16:00 in afternoon weighs and records every group of feed intake and weight of laying eggs.Cleaning up excrement is primary within two days.It is daily during test
The state of mind, the behavior for observing laying hen record every group of daily material consumption, egg production and broken egg amount, and experimental period is 49 days.
4. data processing
Variance analysis and Multiple range test (LSD method) are carried out to data using SPSS statistical software 18.0.
5. testing index
Egg Quality is detected in Henan animal husbandry institute of economics Egg Quality room.
(1) production performance
According to egg production and weighing, count full period feed consumption rate, egg production and egg size, calculate full period average egg production, average egg weight,
Average daily gain, feedstuff-egg ratio and average rate of broken eggs.
The results are shown in Table 3:
3 production performance comparison of test results of table
| Project | Control group | Test group |
| Laying rate (%) | 82.04±0.12b | 84.81±0.16a |
| Egg size | 62.58±1.78b | 66.84±4.71a |
| Feed intake | 125.22±2.10 | 123.08±0.47 |
| Feedstuff-egg ratio | 2.34±0.05a | 2.21±0.02b |
| Rate of broken eggs (%) | 1.70±0.06a | 0.67±0.04b |
Note: the different lowercases after same column numerical value are indicated in 5% horizontal upper significant difference, similarly hereinafter.
(2) Egg Quality
The results are shown in Table 4:
4 Egg Quality comparison of test results of table
| Project | Control group | Test group |
| Eggshell weight (g) | 7.93±0.24b | 8.81±0.91a |
| Eggshell strength | 4.21±0.35 | 4.33±0.37 |
| Yolk color | 6.46±0.57b | 7.50±0.29a |
| Albumen height (mm) | 7.29±0.56 | 7.93±0.81 |
| Protein by weight (g) | 37.30±1.16b | 39.95±2.37a |
| Egg yolk weight (g) | 16.84±1.47 | 17.54±1.01 |
| Hangh unit | 84.50±6.22b | 85.81±6.95a |
From table 3 and table 4:
In layer breeding test, the laying hen for adding feed addictive of the present invention in feeding feed stuff has following variation: laying rate and egg
Weight dramatically increases, and feed intake, feedstuff-egg ratio and rate of broken eggs are substantially reduced, and eggshell weight, eggshell strength, yolk color, albumen are high
Degree, protein by weight and egg yolk weight numerical value increase, i.e. Egg Production of Laying Hens quality significantly improves, and hangh unit numerical value also have it is aobvious
Variation is write, that is, the freshness laid eggs significantly improves.
Embodiment five: influence of the detection feed addictive of layer chicken to laying hen intestinal flora
1. experimental animal and the same example IV of experimental design
2. testing index and method
Testing index: laying hen cecum flora relative abundance and cecum flora structure.
Measuring method: utilizing Illumina HiSeq microarray dataset, carries out to the area gene V3-V4 16S rDNA of bacterium high
Flux sequencing carries out bioinformatic analysis to sequencing result.
Detect unit: the detection of laying hen cecum flora is in Beijing source Nuo Hezhi biological information Science and Technology Ltd., related data
It is provided by the said firm.
3. Egg Production of Laying Hens later period cecum flora relative abundance
For the species composition diversity of study sample, the Tags of all samples is clustered, is gathered sequence with 97% consistency
Class becomes OTUs, then carries out species annotation to the representative sequence of OTUs.It is annotated according to species as a result, choosing and is each grouped in door
The species in the top with abundance maximum in category categorization levels, generate the cumulative figure of species relative abundance cylindricality, intuitively to check
Each sample is in different classifications level, the higher species of relative abundance and its ratio.
In door categorization levels, abundance > 1% cecal content Bacterial community composition situation is as shown in Figure 1.
As shown in Figure 1, abundance greater than 1% have Firmicutes (Firmicutes), Bacteroidetes (Bacteroidetes)、
Fusobacterium door (Fusobacteria), Proteobacteria (Proteobacteria), actinomyces door (Actinobacteria) etc. bacterium
Door.Compared with the control group, the relative abundance of test group Bacteroidetes significantly increase (P<0.05), Firmicutes and Fusobacterium door
Relative abundance significant decrease (P<0.05), Proteobacteria and actinomyces door difference it is not significant (P>0.05).
On belonging to categorization levels, the cecal content Pseudomonas composition situation of abundance > 1% is as shown in Figure 2.
As shown in Figure 2, have between control group and test group 5 Pseudomonas statistical differences it is significant (P<0.05), with control group
It compares, the Bacteroides of test group (Bacteroides) andunidentified_RuminococcaceaeRelative abundance is significant
It increases, fusobacterium (Fusobacterium),Ruminococcaceae_ UCG-005, anaerobic cocci category
(Anaerotruncus) abundance significantly significantly reduces.
4. Egg Production of Laying Hens later period cecum flora structure
Principal component analysis (PCA) is a kind of using variation decomposition, dimensionality reduction is carried out to multidimensional data, to extract in data most
The method of main element and structure.It is analyzed using PCA, two coordinates for utmostly reflecting sample room difference can be extracted
Axis so that the difference of multidimensional data is reflected on two-dimensional coordinate figure, and then discloses the simple rule under complex data background.Such as
Group's composition of fruit sample is more similar, then their distances in PCA figure are closer.PCA analysis using R software ade4 packet with
Ggplot2 software package.
In door categorization levels, Fig. 3 is shown in microbial flora variance analysis between group.
As seen from Figure 3, principal component 1 (PC1) and principal component 2 (PC2) are to cause 2 maximum difference spies of sample
Sign, contribution rate is respectively 32.06% and 20.85%.There are notable difference, control group principal components 2 for control group and test group principal component
Contribution rate is larger, and 1 contribution rate of test group principal component is larger.6 samples of control group are closer, and Relatively centralized, are illustrated pair
It is higher according to the species similarity of 6 samples in group.6 samples distance of test group farther out, illustrates the object of 6 samples in test group
Kind similitude differs greatly.
On belonging to categorization levels, Fig. 4 is shown in microbial flora variance analysis between group.
As seen from Figure 4, principal component 1 (PC1) and principal component 2 (PC2) contribution rate are respectively 18.40% He
14.13%.There are notable differences for control group and test group principal component, and 2 contribution rate of control group principal component is larger, test group principal component 1
Contribution rate is larger.
The present invention is described in detail above in conjunction with embodiment, still, person of ordinary skill in the field can
Understand, without departing from the purpose of the present invention, each design parameter in above-described embodiment can also be changed, shape
At multiple specific embodiments, it is common variation range of the invention, is no longer described in detail one by one herein.
Claims (7)
1. a kind of feed addictive of layer chicken, it is characterised in that: be made of the raw material of following parts by weight: wolfberry leaf, fructus lycii handle, corn
15~25 parts of microbial fermentation product of powder and sesame seed meal, it is 70~80 parts of mealy potato, 2~6 parts of fructus lycii, 0.5~1 part octagonal,
0.1~0.3 part of thyme.
2. feed addictive of layer chicken according to claim 1, it is characterised in that: the preparation method of the microbial fermentation product
Are as follows:
Wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal are taken, is pulverized and sieved, microorganism is added；It is dried after culture, obtains tunning.
3. feed addictive of layer chicken according to claim 2, it is characterised in that: the wolfberry leaf, fructus lycii handle, corn flour and
The mass ratio of sesame seed meal is 0.5~1.5:0.5~1.5:0.1~0.3:0. 05~0.2.
4. feed addictive of layer chicken according to claim 2, it is characterised in that: the microorganism is lactobacillus plantarum
BNCC187897 and/or bacillus subtilis AS1.9086.
5. feed addictive of layer chicken according to claim 2, it is characterised in that: the mass ratio of the microorganism and fermentation liquid
For 5:1.
6. feed addictive of layer chicken according to claim 2, it is characterised in that: the mesh number of the sieving is 50~70 mesh.
7. a kind of preparation method of feed addictive of layer chicken described in claim 1, which comprises the following steps:
(1) wolfberry leaf, fructus lycii handle, corn flour and sesame seed meal are taken, is pulverized and sieved, microorganism is added, dries, obtain after culture
Tunning；
(2) mealy potato is mixed to gained tunning and is uniformly mixed, then sequentially add the star aniseed powder, hundred
In face powder and medlar powder, stirring to be uniformly mixed to get.
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