CN109001448A - A kind of novel purification arsenic chelating type immune complex method - Google Patents
A kind of novel purification arsenic chelating type immune complex method Download PDFInfo
- Publication number
- CN109001448A CN109001448A CN201810610896.3A CN201810610896A CN109001448A CN 109001448 A CN109001448 A CN 109001448A CN 201810610896 A CN201810610896 A CN 201810610896A CN 109001448 A CN109001448 A CN 109001448A
- Authority
- CN
- China
- Prior art keywords
- carrier protein
- solution
- immune complex
- arsenic
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of novel purification arsenic chelating type immune complex methods, the following steps are included: step 1: first preparing chelating agent solution and complete, chelating agent is dissolved in solvent again, chelating agent solution can be obtained, step 2: appropriate carrier protein is taken, carrier protein is added in borate buffer solution, it can be prepared by carrier protein solution, step 3: chelating agent solution obtained is added in carrier protein solution, it is placed in water bath with thermostatic control and stirs evenly, water bath with thermostatic control, mixed liquor can be obtained, step 4: self-control semi-permeable membrane, step 5: pre- purification removes the arsenic ion and other heteroions not chelated, step 6: unbonded carrier protein is removed.Part articles are made by oneself in purification process of the invention, therefore can significantly reduce cost for purification, and the requirement of working environment is lower, and applicable range is more extensive, effectively reduces purification and purification time using compound chromatographic column, reduces costs to a certain extent.
Description
Technical field
The present invention relates to a kind of immune complex field, specially a kind of novel purification arsenic chelating type immune complex side
Method.
Background technique
Immune complex is antibody and a kind of obtained compound of antigen binding, is thin by various immunocytes, phagocytosis
Bacterium, virus, after sensitizer is common dead in conjunction with and formed, so also known as antigen-antibody complex, in some cases,
The immune complex that antigen and antibody are formed in vivo, is deposited on vascular wall basal part, thus activating complement, the benefit being activated
The effects of body, performance dissolution of bacteria, virus, tumour cell, but existing purification arsenic chelating type immune complex method, purification
Higher cost, it is high to the environmental requirement of experimental study in purification process, influence use scope and limit use, and purify the time compared with
It is long, increase the time of purification and purifying.
Summary of the invention
The purpose of the present invention is to provide a kind of novel purification arsenic chelating type immune complex method and preparation method thereof, with
Solve the problems mentioned above in the background art.
To achieve the above object, the invention provides the following technical scheme: a kind of novel purification arsenic chelating type immune complex
Method, comprising the following steps:
Step 1: first chelating agent solution is prepared and is completed, then chelating agent is dissolved in solvent, it is molten that chelating agent can be obtained
Liquid；
Step 2: taking appropriate carrier protein, carrier protein be added in borate buffer solution, can be prepared by carrier egg
White solution；
Step 3: chelating agent solution obtained is added in carrier protein solution, is placed in water bath with thermostatic control and stirs
Uniformly, mixed liquor can be obtained in water bath with thermostatic control；
Step 4: self-control semi-permeable membrane pours into collodion solution into smooth, clean, dry 250ml conical flask, carefully
Rotary container is attached to collodion solution uniformly on wall into a thin layer, pours out extra solution, then by container upside down, into one
Step redundant solution is flow to end and vapors away solvent ether, until touched with finger be formed by film without adhere can, then
Until adding water into container completely, not evaporating dry place in ether after chance water can whiten, and semi-permeable membrane be made, by the film on bottleneck
It disengages, and injects water between film and chamber wall, film is made to automatically disengage bottle wall, gently take out manufactured pouch-shaped semi-permeable membrane, inspection is
No hole should remake if there is cannot then use, can if being immersed in the ethanol water of various concentration with film bag
Obtain the film of different permeabilities, by this method production semi-permeable membrane when not in use, then should deposit in water, so as not to damage；
Step 5: pre- purification removes the arsenic ion and other heteroions not chelated, and 3-5 group semi-permeable membrane is being pressed interior external ordering
EDTA solution is added after arrangement and boils 10min, uses ddH after abandoning waste liquid2O is rinsed, and is repeated the step 2-4 times, mixed in step 3
It closes in the liquid multilayer semi-permeable membrane that is fitted into that treated, uses ddH2O, which dialyses, changes water, after 4 DEG C of dialysed overnights, collects liquid；
Step 6: removing unbonded carrier protein, takes not used compound chromatographic column, is rinsed and is chromatographed with dilution buffer
The pipeline of column is packed into the filler that can be specifically bound with arsenic ion in the chromatographic column of upper layer, and being packed into lower layer's chromatographic column can be with
The filler of carrier protein specific binding continues to use dilution buffer K after filling column2HPO4Chromatographic column is balanced, it is flat to chromatographic column
After weighing apparatus, with dilution buffer K2HPO4Sample in dilution step four, then upper prop, carrier protein and filler specifically bind, make
With dilution buffer K2HPO4It rinses chromatographic column to baseline to balance, then be washed using the phosphate solution of 0.05-0.1mol/L
De-, the eluent collected is packed into semi-permeable membrane, uses ddH2O dialysis, after changing water 2-4 times, 4 DEG C of dialysed overnights collect sample,
The arsenic chelating type immune complex of high-purity can be obtained.
Preferably, zinc fingers, sulfydryl, cysteine are at least contained in the structure of the immune complex or carrier protein
One of residue, arsenic ion are mutually tied with immune complex or carrier protein with zinc fingers or sulfydryl or cysteine residues
It closes.
Preferably, the carrier protein is antibody protein, lipoprotein, hemoglobin, nuclear antigen, foreign sera antigen, disease
Poison, bacterium, protozoon, worm or immunoglobulin one of.
Preferably, the arsenic ion is specifically bound with energy and carrier protein again after passing through chelating agent in conjunction with carrier protein
Antibody combine, the chelating agent is ITCBE, EDTA, polyphosphate, amino carboxylic acid, 1,3- diketone, one in hydroxycarboxylic acid
Kind.
A kind of method of quantitative detection arsenic chelating type CIC ELISA, it is immune with the above-mentioned arsenic chelating type of known content
Compound is detected: enzyme-linked immunization, enzyme linked immunological and Atomic absorption as standard items using a pair of sample of following methods
Spectrum combined techniques, enzyme linked immunological and inductivity coupled plasma mass spectrometry combined techniques, purification immune complex and atomic absorption spectrum
Combined techniques, purification immune complex and inductivity coupled plasma mass spectrometry combined techniques, electrophoresis and enzyme linked immunological or atomic absorption light
Spectrum or inductivity coupled plasma mass spectrometry combined techniques.
Compared with prior art, the beneficial effects of the present invention are: part articles are made by oneself in purification process of the invention, therefore
Cost for purification can be significantly reduced, the requirement to working environment is lower, and applicable range is more extensive, has using compound chromatographic column
Effect reduces the time of purification and purifying, reduces costs to a certain extent.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The present invention provides a kind of technical solution: a kind of novel purification arsenic chelating type immune complex method, including following step
It is rapid:
Step 1: first chelating agent solution is prepared and is completed, then chelating agent is dissolved in solvent, it is molten that chelating agent can be obtained
Liquid；
Step 2: taking appropriate carrier protein, carrier protein be added in borate buffer solution, can be prepared by carrier egg
White solution；
Step 3: chelating agent solution obtained is added in carrier protein solution, is placed in water bath with thermostatic control and stirs
Uniformly, mixed liquor can be obtained in water bath with thermostatic control；
Step 4: self-control semi-permeable membrane pours into collodion solution into smooth, clean, dry 250ml conical flask, carefully
Rotary container is attached to collodion solution uniformly on wall into a thin layer, pours out extra solution, then by container upside down, into one
Step redundant solution is flow to end and vapors away solvent ether, until touched with finger be formed by film without adhere can, then
Until adding water into container completely, not evaporating dry place in ether after chance water can whiten, and semi-permeable membrane be made, by the film on bottleneck
It disengages, and injects water between film and chamber wall, film is made to automatically disengage bottle wall, gently take out manufactured pouch-shaped semi-permeable membrane, inspection is
No hole should remake if there is cannot then use, can if being immersed in the ethanol water of various concentration with film bag
Obtain the film of different permeabilities, by this method production semi-permeable membrane when not in use, then should deposit in water, so as not to damage；
Step 5: pre- purification removes the arsenic ion and other heteroions not chelated, and 3-5 group semi-permeable membrane is being pressed interior external ordering
EDTA solution is added after arrangement and boils 10min, uses ddH after abandoning waste liquid2O is rinsed, and repeats the step 3 time, the mixing in step 3
In liquid is fitted into that treated multilayer semi-permeable membrane, ddH is used2O, which dialyses, changes water, after 4 DEG C of dialysed overnights, collects liquid；
Step 6: removing unbonded carrier protein, takes not used compound chromatographic column, is rinsed and is chromatographed with dilution buffer
The pipeline of column is packed into the filler that can be specifically bound with arsenic ion in the chromatographic column of upper layer, and being packed into lower layer's chromatographic column can be with
The filler of carrier protein specific binding continues to use dilution buffer K after filling column2HPO4Chromatographic column is balanced, it is flat to chromatographic column
After weighing apparatus, with dilution buffer K2HPO4Sample in dilution step four, then upper prop, carrier protein and filler specifically bind, make
With dilution buffer K2HPO4It rinses chromatographic column to baseline to balance, then be washed using the phosphate solution of 0.05-0.1mol/L
De-, the eluent collected is packed into semi-permeable membrane, uses ddH2O dialysis, after changing water 3 times, 4 DEG C of dialysed overnights collect sample, i.e.,
The arsenic chelating type immune complex of high-purity can be obtained.
Further, at least residual containing zinc fingers, sulfydryl, cysteine in the structure of immune complex or carrier protein
One of base, arsenic ion are combined with immune complex or carrier protein with zinc fingers or sulfydryl or cysteine residues.
Further, carrier protein be antibody protein, lipoprotein, hemoglobin, nuclear antigen, foreign sera antigen, virus,
Bacterium, protozoon, worm or immunoglobulin one of.
Further, it is specifically bound again with energy and carrier protein after arsenic ion passes through chelating agent in conjunction with carrier protein
Antibody combines, one of chelating agent ITCBE, EDTA, polyphosphate, amino carboxylic acid, 1,3- diketone, hydroxycarboxylic acid.
It is above-mentioned with known content the present invention also provides a kind of method of quantitative detection arsenic chelating type CIC ELISA
Arsenic chelating type immune complex is detected as standard items using a pair of sample of following methods: enzyme-linked immunization enzyme-linked is exempted from
Epidemic disease and atomic absorption spectrum combined techniques, enzyme linked immunological and inductivity coupled plasma mass spectrometry combined techniques, purification immune complex with
Atomic absorption spectrum combined techniques, purification immune complex and inductivity coupled plasma mass spectrometry combined techniques, electrophoresis and enzyme linked immunological
Or atomic absorption spectrum or inductivity coupled plasma mass spectrometry combined techniques.
Unless otherwise indicated, the laboratory operating procedures being related to are the step of this field routine to the present invention, and reagent, material are such as
It is following cited, do not enumerate in the present invention come be commonly used in the art or can be obtained by commercially available mode:
Dilution buffer is the 0.05M carbonate buffer solution of pH9.6, preparation method example: takes the K of 1.5g2CO3And 1.93g
KHCO3Dissolution plus ddH2O is settled to 1000mL；
Washing buffer is the 0.15MPBS buffer of pH7.4, preparation method example: takes the KH of 0.2g2PO4, 2.90g
Na2HPO4·12H2O, KCl, 0.5mLTween-20 of NaCl, 0.2g of 8.0g, dissolution plus ddH2O is settled to 1000mL；
Confining liquid is bovine serum albumin solution, and preparation method example: taking 0.1g bovine serum albumin(BSA), and washing buffer is added
Liquid dilution is settled to 100mL；
Terminate liquid is 2MH2Preparation method example: SO4 takes the ddH of 178.3mL2O, to ddH2It is added dropwise along wall in O dense
H2SO4, it is stirring while adding, it is settled to 200mL；
The pH of substrate buffer solution is 5.0, Na2HPO4Molar concentration be 0.2M, the molar concentration of citric acid is 0.1M, often
The substrate buffer solution of 50mL the preparation method is as follows: taking 1.42gNa2HPO4, 0.96g citric acid, ddH is then added2O to 50mL,
To obtain the final product；
Substrate is methyl biphenyl amine (TMB) solution, and methyl biphenyl amine (TMB) solution is by the group distribution according to following ratio
It makes: TMB: substrate buffer solution: 0.75%H2O2=0.5mL:10mL:32 μ L, wherein TMB is the methyl biphenyl amine second of 2g/L
Alcoholic solution；
The albumen that immune complex can be captured is the albumen that can be specifically bound with immune complex, including but not limited to
Such as C1Q, CIF albumen, anti-C_3 antibody；In following embodiment, can capture immune complex albumen it is specifically used be
C1qRecombinantProtein, article No. are " NOVUSH00000712-p01 "；
The substance of capture arsenic is the anti-AsmAb of mouse that article No. is " Guangzhou Ran Ke company RK15728 ", in following implementation
With the substance of arsenic specific binding, the substance, secondary antibody, anti-arsenic antibody that have affinity with arsenic be capture arsenic substance；
The antibody that can be specifically bound with carrier protein is rabbit-anti people carrier protein antibodies, is commercially available.
Dilution multiple proportions is w/v below.
Method one: purification CIC method+ELISA method detection arsenic chelating type immune complex, the specific steps are as follows:
1) it extracts nospecific immunity compound: utilizing the polyethylene glycol PEG precipitation method, ultracentrifugation, molecule ultrafiltration, gel
The methods of filtering is extracted the immune complex that nospecific immunity compound comes out purification and is redissolved；
2) it is coated on solid phase carrier with the substance that can capture arsenic: diluting coated antibody to 15500- with sample buffer
100000 times, be added elisa plate micropore in, 4 DEG C overnight 16-18 hour or 37 DEG C water-bath 1-3 hours, storage refrigerator；
3) it closes: removing sample buffer dilution, and washed with cleaning solution, after the completion of washing, add confining liquid, 37
DEG C place 1 hour, remove confining liquid, and washed with cleaning solution, after the completion of washing, elisa plate in 36.7-37.5 DEG C place
1 hour；
4) add measuring samples, and incubate: sampling, make to be checked from the redissolution liquid of the nospecific immunity compound of extraction
Sample；Make standard sample with the arsenic chelating type immune complex of known content；10-40 times is diluted with sample buffer, is added micro-
Kong Zhong, 37 DEG C effect 1-2.5 hours；
5) enzyme conjugates incubates: the redissolution liquid of nospecific immunity compound is removed, and is washed with cleaning solution, it is to be washed
After the completion of washing, be added use the diluted HRP enzyme labelled antibody of dilution buffer, 37 DEG C effect 1.5-2 hours, keep it anti-with anti-arsenic antibody
It answers；
6) substrate incubates: enzyme labelled antibody is removed, and is washed with cleaning solution, after the completion of washing, and addition substrate, 37 DEG C
It is protected from light effect 30 minutes；
7) it terminates reaction: terminate liquid is added dropwise to each micropore with speed identical with substrate solution is added and sequence.
8) taking wavelength is 405nm, after adding terminate liquid, takes 0.5ml liquid in the solution that elutes in above-mentioned ELISA agent plate
Body, in the OD value for reading sample to be tested group and standard sample in microplate reader respectively, by acquiring to be measured compared with standard sample group
The content (microplate reader can also not used, directly carry out qualitative detection by staining conditions) of sample.
Method two: purification CIC method+AAS method detects arsenic chelating type immune complex, the specific steps are as follows:
1) it extracts nospecific immunity compound: utilizing the polyethylene glycol PEG precipitation method, ultracentrifugation, molecule ultrafiltration, gel
The methods of filtering is extracted the immune complex that nospecific immunity compound comes out purification and is redissolved；
2) it detects: taking 0.5ml liquid from the redissolution liquid of nospecific immunity compound, detected in Atomic Absorption Spectrometer
It chelates in the arsenic on immune complex, reads respective value.
Part articles are made by oneself in purification process of the invention, therefore can significantly reduce cost for purification, to working environment
It is required that lower, applicable range is more extensive, the time of purification and purifying is effectively reduced using compound chromatographic column, to a certain extent
It reduces costs.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of novel purification arsenic chelating type immune complex method, which comprises the following steps:
Step 1: first chelating agent solution is prepared and is completed, then chelating agent is dissolved in solvent, chelating agent solution can be obtained；
Step 2: appropriate carrier protein is taken, carrier protein is added in borate buffer solution, it is molten to can be prepared by carrier protein
Liquid；
Step 3: chelating agent solution obtained being added in carrier protein solution, is placed in water bath with thermostatic control and stirs evenly,
Mixed liquor can be obtained in water bath with thermostatic control；
Step 4: self-control semi-permeable membrane pours into collodion solution into smooth, clean, dry 250ml conical flask, careful to rotate
Container is attached to collodion solution uniformly on wall into a thin layer, pours out extra solution, then by container upside down, further will
Redundant solution is flow to end and vapors away solvent ether, until touched with finger be formed by film without adhere can, then toward hold
Until adding water in device completely, not evaporating dry place in ether after chance water can whiten, and semi-permeable membrane is made, the film on bottleneck is taken off
It opens, and injects water between film and chamber wall, film is made to automatically disengage bottle wall, gently take out manufactured pouch-shaped semi-permeable membrane, check whether
Hole should remake if there is cannot then use, if being immersed in the ethanol water of various concentration with film bag, can obtain
To the film of different permeabilities, by the production of this method semi-permeable membrane when not in use, then should deposit in water, in order to avoid damage；
Step 5: pre- purification removes the arsenic ion and other heteroions not chelated, and 3-5 group semi-permeable membrane is being pressed inside and outside sequential arrangement
EDTA solution is added afterwards and boils 10min, uses ddH after abandoning waste liquid2O is rinsed, and is repeated the step 2-4 times, the mixed liquor in step 3
In the multilayer semi-permeable membrane that is fitted into that treated, ddH is used2O, which dialyses, changes water, after 4 DEG C of dialysed overnights, collects liquid；
Step 6: removing unbonded carrier protein, takes not used compound chromatographic column, rinses chromatographic column with dilution buffer
Pipeline is packed into the filler that can be specifically bound with arsenic ion in the chromatographic column of upper layer, energy and carrier is packed into lower layer's chromatographic column
The filler that protein-specific combines continues to use dilution buffer K after filling column2HPO4Chromatographic column is balanced, after chromatographing column equilibration,
With dilution buffer K2HPO4Sample in dilution step four, then upper prop, carrier protein and filler are specifically bound, and use is dilute
Release buffer K2HPO4It rinses chromatographic column to baseline to balance, then be eluted using the phosphate solution of 0.05-0.1mol/L,
Obtained eluent is collected, semi-permeable membrane is packed into, uses ddH2O dialysis, after changing water 2-4 times, 4 DEG C of dialysed overnights collect sample
Obtain the arsenic chelating type immune complex of high-purity.
2. arsenic chelating type immune complex according to claim 1, it is characterised in that: the immune complex or carrier egg
At least contain one of zinc fingers, sulfydryl, cysteine residues, arsenic ion and immune complex or carrier in white structure
Albumen is combined with zinc fingers or sulfydryl or cysteine residues.
3. arsenic chelating type immune complex according to claim 1, it is characterised in that: the carrier protein is antibody egg
Among white, lipoprotein, hemoglobin, nuclear antigen, foreign sera antigen, virus, bacterium, protozoon, worm or immunoglobulin
It is a kind of.
4. arsenic chelating type immune complex according to claim 1, which is characterized in that the arsenic ion by chelating agent with
Carrier protein combine after again with can and the antibody of carrier protein specific binding in conjunction with, the chelating agent is ITCBE, EDTA, more
One of phosphate, amino carboxylic acid, 1,3- diketone, hydroxycarboxylic acid.
5. a kind of method of qualitative detection arsenic chelating type CIC ELISA, which is characterized in that wanted with the right of known content
Arsenic chelating type immune complex described in asking 1 is detected: enzyme linked immunological as standard items using a pair of sample of following methods
Method, enzyme linked immunological and atomic absorption spectrum combined techniques, enzyme linked immunological and inductivity coupled plasma mass spectrometry combined techniques, purification are immune
Compound and atomic absorption spectrum combined techniques, purification immune complex and inductivity coupled plasma mass spectrometry combined techniques, electrophoresis and
Enzyme linked immunological or atomic absorption spectrum or inductivity coupled plasma mass spectrometry combined techniques.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810610896.3A CN109001448A (en) | 2018-06-14 | 2018-06-14 | A kind of novel purification arsenic chelating type immune complex method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810610896.3A CN109001448A (en) | 2018-06-14 | 2018-06-14 | A kind of novel purification arsenic chelating type immune complex method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109001448A true CN109001448A (en) | 2018-12-14 |
Family
ID=64600300
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810610896.3A Pending CN109001448A (en) | 2018-06-14 | 2018-06-14 | A kind of novel purification arsenic chelating type immune complex method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109001448A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112881674A (en) * | 2021-02-26 | 2021-06-01 | 上海市农产品质量安全中心 | Arsenic ion detection kit and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004294329A (en) * | 2003-03-27 | 2004-10-21 | Hitachi Constr Mach Co Ltd | Method and apparatus for analyzing amount of elution of heavy metals contained in soil, and sample for the same |
| JP2008232766A (en) * | 2007-03-19 | 2008-10-02 | Central Res Inst Of Electric Power Ind | Immunological method and device for quantitatively determining metal and metal complex-immobilized membrane used for these |
| US20140248184A1 (en) * | 2011-04-04 | 2014-09-04 | Arkray, Inc. | Method for Recovering Metal and Kit for Recovery of Metal for Use in the Same |
| CN105044324A (en) * | 2015-07-14 | 2015-11-11 | 上海拜豪生物科技有限公司 | Arsenic-chelated immune compound, and preparation method and application of compound |
-
2018
- 2018-06-14 CN CN201810610896.3A patent/CN109001448A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004294329A (en) * | 2003-03-27 | 2004-10-21 | Hitachi Constr Mach Co Ltd | Method and apparatus for analyzing amount of elution of heavy metals contained in soil, and sample for the same |
| JP2008232766A (en) * | 2007-03-19 | 2008-10-02 | Central Res Inst Of Electric Power Ind | Immunological method and device for quantitatively determining metal and metal complex-immobilized membrane used for these |
| US20140248184A1 (en) * | 2011-04-04 | 2014-09-04 | Arkray, Inc. | Method for Recovering Metal and Kit for Recovery of Metal for Use in the Same |
| CN105044324A (en) * | 2015-07-14 | 2015-11-11 | 上海拜豪生物科技有限公司 | Arsenic-chelated immune compound, and preparation method and application of compound |
Non-Patent Citations (1)
| Title |
|---|
| 黄桂萍 等: "Fe(OH)3溶胶及其纯化半透膜制备的探讨", 《赣南师范学院学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112881674A (en) * | 2021-02-26 | 2021-06-01 | 上海市农产品质量安全中心 | Arsenic ion detection kit and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Conradie et al. | ELISA solid phase: partial denaturation of coating antibody yields a more efficient solid phase | |
| CN110068688A (en) | Flow measurement chromatography detection card is immunized in lactoferrin competition law nano flower in a kind of cow's milk | |
| CN102426239A (en) | Colloidal gold chromatographic test strip for rapidly testing lead ion | |
| CN108646029B (en) | Human anti-Gal-IgG antibody detection kit and preparation method and application thereof | |
| CN109001448A (en) | A kind of novel purification arsenic chelating type immune complex method | |
| CN101046479B (en) | Process of preparing human serum base matter containing no target protein | |
| CN108982864A (en) | A kind of novel purification mercury chelating type immune complex method | |
| CN108535496A (en) | A kind of novel purification lead chelating type immune complex method | |
| CN108802363A (en) | A kind of novel purification cadmium chelating type immune complex method | |
| CN105738611A (en) | Benzo(a)pyrene enzyme-linked immunosorbent assay (ELISA) kit and detection method thereof | |
| CN104965076B (en) | Cadmium-chelated immune complex as well as preparation method and application thereof | |
| WO2013077332A1 (en) | Method for detecting matrix metalloproteinase-3 by latex agglutination method | |
| CN105044324B (en) | Arsenic-chelated immune compound, and preparation method and application of compound | |
| CN102621311B (en) | Colloidal gold chromatography anti-SSB antibody detection test paper and preparation method thereof | |
| CN102621313B (en) | Colloidal gold chromatography anti-Sp100 antibody detection test paper and preparation method thereof | |
| CN108828207A (en) | A kind of excellent effect cadmium chelating type immune complex and preparation method thereof | |
| CN106248955A (en) | A kind of test kit detecting humanization PD L1 antibody | |
| CN108663528A (en) | A kind of excellent effect lead chelating type immune complex and preparation method thereof | |
| CN108802362A (en) | A kind of excellent effect arsenic chelating type immune complex and preparation method thereof | |
| CN108761060A (en) | A kind of excellent effect mercury chelating type immune complex and preparation method thereof | |
| CN105021548B (en) | A kind of arsenic fibrinogen chelate and its preparation method and application | |
| CN103374048A (en) | Streptomycin hapten, as well as preparation method and application thereof | |
| CN102585009A (en) | Monoclonal antibody, enzyme-linked immunosorbent assay (ELISA) method and kit for detecting chloramphenicol residues | |
| CN105021554B (en) | A kind of arsenic-IgM chelate and its preparation method and application | |
| CN104987405B (en) | A kind of arsenic IgG chelates and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181214 |
|
| WD01 | Invention patent application deemed withdrawn after publication |