CN108990467A - A method of promote yellow bean sprout to sprout using chitosan oligosaccharide - Google Patents
A method of promote yellow bean sprout to sprout using chitosan oligosaccharide Download PDFInfo
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- CN108990467A CN108990467A CN201810878906.1A CN201810878906A CN108990467A CN 108990467 A CN108990467 A CN 108990467A CN 201810878906 A CN201810878906 A CN 201810878906A CN 108990467 A CN108990467 A CN 108990467A
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- Prior art keywords
- sprout
- soya bean
- chitosan oligosaccharide
- seed
- yellow bean
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention provides a kind of method for promoting yellow bean sprout to sprout using chitosan oligosaccharide.Chitosan oligosaccharide is added by the soybean soaking stage, improves germinating energy, germination percentage and the germination index of soya bean seed, while increasing the nutritional quality of yellow bean sprout after sprouting.Using the yield and quality method of the invention, it is possible to improve yellow bean sprout, production efficiency is improved, and this method is easy to operate, it is low in cost, without increasing equipment, it is convenient for commercial introduction application.
Description
Technical field
The present invention relates to yellow bean sprout production field, in particular to a kind of chitosan oligosaccharide impregnates soya bean seed and yellow bean sprout is promoted to sprout
New method.
Background technique
Soya bean is native to China, is one of important cereal crops in China.In soya bean protein rich in, amino acid with
And vitamin, unsaturated fatty acid and minerals etc., but because its tissue is hard, part nutritional ingredient is not easy to be absorbed and used.
And while retaining original nutrition, the ingredient absorbed: soluble egg is easy containing more human body compared to for soya bean for yellow bean sprout
White matter, reduced sugar, free amino acid etc.；And in yellow bean sprout also containing phytosterol, γ-aminobutyric acid, polyphenol, flavones, saponin(e,
And the physiological activators such as various vitamins (especially vitamin C) and microelement, become the tradition food being favored by people
Product.In order to promote soya bean germination and improve yellow bean sprout quality in production, each plant growth regulators such as gibberellin etc. was once
It is applied in soya bean germination process.However, the excess residual of plant growth regulator can generate threat to people and animals and environment, and
And there is also many control unknown risks, and so with the raising of people's Consciousness of food security, the plant growth regulator that uses in the past
It is all disabled.
Chitosan oligosaccharide be in presently found nature it is unique it is positively charged, in alkalinity, water-soluble polysaccharide, have superior
Bioactivity.In addition, chitosan oligosaccharide can be prepared by chitin, chitosan, it is cheap, and there is non-toxic degradable and biofacies
The advantages that capacitive is good, therefore chitosan oligosaccharide is worth with huge applications.The present invention adds chitosan oligosaccharide in soya bean seed impregnation stage
Yellow bean sprout is promoted to sprout, application meets food safety and environmental requirement.
Summary of the invention
Present invention is primarily aimed at a kind of method for promoting yellow bean sprout to sprout using chitosan oligosaccharide is provided, this method is in Huang
Beans seed impregnation stage adds chitosan oligosaccharide to promote soybean germination, while improving yellow bean sprout nutritional quality.
The purpose of the present invention is achieved through the following technical solutions:
A method of yellow bean sprout is improved using chitosan oligosaccharide and is sprouted, and the specific operation method is as follows:
(1) soya bean seed impregnates: soya bean seed being immersed in 1~2.5 times of soya bean seed quality of water, it is small to impregnate 3~5
When；
Chitosan oligosaccharide is added during soya bean seed impregnates, the mass ratio of the chitosan oligosaccharide and soya bean seed is 1:
10000000~1:10000；
(2) it germinates: the soya bean seed after immersion is placed in dark culture in the environment of 23~28 DEG C of temperature, 80% or more humidity
7~9 days.Specifically:
Soya bean seed after immersion is placed in the culture dish for being covered with 1~3 layer of wet filter paper, is covered on culture dish wet
1~3 layer of gauze, keep 23~28 DEG C of temperature, 80% or more humidity adds water every 10~15h and cleans gauze, dark culture 7
~9 days.
According to technical solution above, in preferred situation, the molecular weight of the chitosan oligosaccharide is 1000~3000Da, water-soluble
Well.
According to technical solution above, in preferred situation, the soya bean seed after step (1) is impregnated is rushed with tap water
It washes.
According to technical solution above, in preferred situation, the soya bean seed is full seed, no disease and pests harm, size one
The soya bean seed of cause.
According to technical solution above, in preferred situation, the mass ratio of chitosan oligosaccharide and soya bean seed described in step (1) is
1:1000000~1:100000.
According to technical solution above, the soya bean seed after immersion is placed in preferred situation, in step (2) be covered with it is wet
2 layers of filter paper culture dish in, cover 2 layers of wet gauze on culture dish, kept for 23~28 DEG C of temperature, 80% or more humidity,
Every 12h plus water and gauze is cleaned, dark culture 7 days.
Beneficial effects of the present invention:
Protrusion effect of the invention is in identical germinating time, and the soya bean ratio of seed impregnation stage addition chitosan oligosaccharide does not add
Soybean germination gesture, germination percentage and the germination index added significantly improves, and fresh weight and the bud length of the yellow bean sprout of preparation dramatically increase, soya bean
The yield of bud significantly improves, while free amino acid in yellow bean sprout, reduced sugar, the content of vitamin C and condensed tannin are also significant
Increase, the nutritional quality of yellow bean sprout significantly improves.The innovation of the invention consists in that external source addition chitosan oligosaccharide promotes sprouting for soya bean
Hair, fresh weight and the bud for increasing yellow bean sprout are long, the main nutrient composition of yellow bean sprout are improved, so as to improve the production of yellow bean sprout
Amount and quality improve production efficiency, and this method is easy to operate, low in cost, without increasing equipment, are convenient for commercial introduction
Using.
Specific embodiment
Following nonlimiting examples can with a person of ordinary skill in the art will more fully understand the present invention, but not with
Any mode limits the present invention.
Test involved in the following embodiments of the present invention is as follows, separately sampled measurement:
1. grain number of germinateing daily: with the prominent 1mm or so that breaks in the seed coat of radicle for sprouting standard.
2. subnumber × 100 are planted experimentally in germinating energy (%)=3 day chitting piece number/confession.
3. subnumber × 100 are planted experimentally in germination percentage (%)=7 day chitting piece number/confession.
4. germination index (GI)=Σ (Gt/Dt), wherein Gt is germinative number, Dt is corresponding germination number of days.
5. fresh weight: weighing the weight of each yellow bean sprout with a ten thousandth precision balance.
6. bud is long: the length of hypocotyl base portion to bud top.
7. free amino acid: being measured using glycine as standard items using ninhydrin.
8. reduced sugar: using DNS colorimetric method for determining.
9. Vitamin C content measures: utilizing 2,6- dichloroindophenol titration.
10. condensed tannin: using vanillic aldehyde-hydrochloric acid colorimetric method for determining by standard items of catechin.
Embodiment 1
Soya bean seed impregnates: choosing full seed, no disease and pests harm, soya bean seed of the same size, weighs 200 grams, immerses
In 500 milliliters of water, impregnates 4 hours, then rinsed 3 times with tap water.0.2 milligram of shell widow is added in soya bean seed soaking water
Sugar, the molecular weight of chitosan oligosaccharide are 1000Da.
Germination: the soya bean seed after taking 350 immersions at random is respectively placed in 5 culture dishes for being covered with 2 layers of wet filter paper
In, 2 layers of wet gauze are covered on culture dish, temperature is maintained at 25 DEG C, and humidity 85% every 12h plus water and cleans gauze, secretly
Culture 7 days.
As shown in Table 1, after the method processing, germinating energy, germination percentage and the germination index of soya bean seed are significantly improved, preparation
Yellow bean sprout fresh weight and bud length also obviously increase, while free amino acid, reduced sugar, vitamin C and condensed tannin in yellow bean sprout
Content also dramatically increase, the nutritional quality of yellow bean sprout significantly improves.
Embodiment 2
Soya bean seed impregnates: choosing full seed, no disease and pests harm, soya bean seed of the same size, weighs 200 grams, immerses
In 500 milliliters of water, impregnates 4 hours, then rinsed 3 times with tap water.2 milligrams of chitosan oligosaccharides are added in soybean soaking water, shell is few
The molecular weight of sugar is 3000Da.
Germination: the soya bean seed after taking 350 immersions at random is respectively placed in 5 culture dishes for being covered with 2 layers of wet filter paper
In, 2 layers of wet gauze are covered on culture dish, temperature is maintained at 25 DEG C, and humidity 85% every 12h plus water and cleans gauze, secretly
Culture 7 days.
As shown in Table 1, after the method processing, germinating energy, germination percentage and the germination index of soya bean seed are significantly improved, preparation
Yellow bean sprout fresh weight and bud length also obviously increase, while free amino acid, reduced sugar, vitamin C and condensed tannin in yellow bean sprout
Content also dramatically increase, the nutritional quality of yellow bean sprout significantly improves.
Embodiment 3 (blank sample)
Soya bean seed impregnates: choosing full seed, no disease and pests harm, soya bean seed of the same size, weighs 200 grams, immerses
In 500 milliliters of water, impregnates 4 hours, then rinsed 3 times with tap water.
Germination: the soya bean seed after taking 350 immersions at random is respectively placed in 5 culture dishes for being covered with 2 layers of wet filter paper
In, 2 layers of wet gauze are covered on culture dish, temperature is maintained at 25 DEG C, and humidity 85% every 12h plus water and cleans gauze, secretly
Culture 7 days.
1 chitosan oligosaccharide of table is sprouted to yellow bean sprout and the influence of nutritional quality parameter
For any person skilled in the art, without departing from the scope of the technical proposal of the invention, all
Many possible changes and modifications are made to technical solution of the present invention using the technology contents of the disclosure above, or are revised as equivalent
The equivalent embodiment of variation.Therefore, anything that does not depart from the technical scheme of the invention, according to the technical essence of the invention to
Any simple modifications, equivalents, and modifications that upper embodiment is done should all still fall within the range of technical solution of the present invention protection
It is interior.
Claims (4)
1. a kind of method for promoting yellow bean sprout to sprout using chitosan oligosaccharide, which is characterized in that specific step is as follows:
(1) soya bean seed impregnates: soya bean seed being immersed in 1~2.5 times of soya bean seed quality of water, is impregnated 3~5 hours；
Be added chitosan oligosaccharide in soya bean seed soaking process, the mass ratio of the chitosan oligosaccharide and soya bean seed be 1:10000000~
1:10000；
(2) it germinates: the soya bean seed after immersion is placed in dark culture 7~9 in the environment of 23~28 DEG C of temperature, 80% or more humidity
It.
2. the method according to claim 1 for promoting yellow bean sprout to sprout using chitosan oligosaccharide, which is characterized in that the chitosan oligosaccharide
Molecular weight be 1000~3000Da.
3. the method according to claim 1 for promoting yellow bean sprout to sprout using chitosan oligosaccharide, which is characterized in that pass through step
(1) the soya bean seed after impregnating is rinsed with tap water.
4. the method according to claim 1 for promoting yellow bean sprout to sprout using chitosan oligosaccharide, which is characterized in that the soya bean kind
Son is full seed, no disease and pests harm, soya bean seed of the same size.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810878906.1A CN108990467A (en) | 2018-08-03 | 2018-08-03 | A method of promote yellow bean sprout to sprout using chitosan oligosaccharide |
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| CN201810878906.1A CN108990467A (en) | 2018-08-03 | 2018-08-03 | A method of promote yellow bean sprout to sprout using chitosan oligosaccharide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111819952A (en) * | 2020-07-29 | 2020-10-27 | 上饶师范学院 | Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes |
| CN113647316A (en) * | 2021-08-17 | 2021-11-16 | 大连工业大学 | Method for promoting bean sprout germination by using algin oligosaccharide |
| CN114467407A (en) * | 2022-01-04 | 2022-05-13 | 海南大学 | Method for economically and rapidly breaking dormancy of acacia longissima seeds and promoting germination of acacia longissima seeds |
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| CN105746517A (en) * | 2011-09-23 | 2016-07-13 | 诺维信生物农业公司 | Chitooligosaccharides And Methods For Use In Enhancing Soybean Growth |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111819952A (en) * | 2020-07-29 | 2020-10-27 | 上饶师范学院 | Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes |
| CN113647316A (en) * | 2021-08-17 | 2021-11-16 | 大连工业大学 | Method for promoting bean sprout germination by using algin oligosaccharide |
| CN114467407A (en) * | 2022-01-04 | 2022-05-13 | 海南大学 | Method for economically and rapidly breaking dormancy of acacia longissima seeds and promoting germination of acacia longissima seeds |
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Application publication date: 20181214 |