CN108982480B - Chemiluminescence detection method and device, chemiluminescence immunoassay analyzer and storage medium - Google Patents
Chemiluminescence detection method and device, chemiluminescence immunoassay analyzer and storage medium Download PDFInfo
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Abstract
The invention discloses a chemiluminescence detection method for judging whether a tip head is loaded successfully, which comprises the following steps: acquiring a reference light transmission amount; controlling the injector loading suction head to move to a detection position and acquiring the light transmittance of the suction head at the detection position; and calculating the ratio of the light transmission quantity of the detection position to the stored reference light transmission quantity and judging whether the suction head is loaded at the detection position according to the size of the ratio. According to the chemiluminescence detection method, whether the tip head is loaded successfully or not is judged by adopting ratio calculation, the accuracy of tip head loading judgment is guaranteed, and the method can adapt to the attenuation of each component of an instrument, so that the requirement on a hardware board card is reduced, and the instability of detection results caused by factors such as the aging, differentiation or loading of a photoelectric module such as an LED light source or a photoelectric receiver is avoided. The invention also provides a chemiluminescence detection device, a chemiluminescence immunoassay analyzer and a readable storage medium with the same technical effect.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnosis, in particular to a chemiluminescence detection method and device, a chemiluminescence immunoassay analyzer and a storage medium.
Background
When in-vitro diagnostic equipment such as a chemiluminescence immunoassay analyzer detects specific components in a blood sample, a reagent strip is pricked after a suction head (tip head) of an injector device to carry out corresponding related item detection, and the operation flow of a general analyzer is automatic except for manual placement of the tip head.
However, in the process of actual detection work of the analyzer, if a tip head is not placed in a certain detection channel due to misoperation during manual tip head placement, or the tip head is not successfully loaded and lifted due to reasons such as component assembly performance, even if the tip head of an injector which has successfully loaded the tip head falls in the moving process, if the analyzer cannot timely and accurately judge the loading state of the tip head, the analyzer cannot be guaranteed to smoothly perform subsequent related detection steps such as controlling the tip head to suck a sample, and the like, so that the stability and reliability of sample related item detection are affected.
Disclosure of Invention
The invention aims to overcome the technical defects and provide a chemiluminescence detection method and device, a chemiluminescence immunoassay analyzer and a storage medium so as to accurately and timely judge whether a tip (tip) is successfully loaded.
In order to achieve the above technical object, a technical solution of the present invention provides a chemiluminescence detection method, which comprises:
acquiring a reference light transmission amount;
controlling the injector loading suction head to move to a detection position and acquiring the light transmittance of the suction head at the detection position;
and calculating the ratio of the light transmission quantity of the detection position to the stored reference light transmission quantity and judging whether the suction head is loaded at the detection position according to the size of the ratio.
Preferably, before the suction head is loaded at the judgment detection position, the method further comprises the following steps:
acquiring the placing light transmission quantity of the placed suction head at the placing position of the suction head;
and calculating the ratio of the placed light transmission quantity to the stored reference light transmission quantity and judging whether the sucker is placed according to the ratio.
Preferably, before the suction head is loaded at the judgment detection position, the method further comprises the following steps:
controlling the injector to obtain the lifting light transmittance of the placing position after loading the lifting sucker;
and calculating the ratio of the lifting light transmission quantity to the stored reference light transmission quantity and judging whether the sucker is loaded according to the ratio.
Preferably, the method further comprises the following steps after loading the pipette tip:
after the suction head is moved to a sample suction position for sample suction, obtaining the sample suction light transmittance of the sucked sample suction head;
and calculating the ratio of the sample suction transmission amount to the stored reference transmission amount and judging the type of the sample according to the ratio.
Preferably, the chemiluminescence detection method further comprises the following steps: and acquiring a new reference light transmission amount at any time when no suction head is placed at the placing position of the suction head, and updating and replacing the stored reference light transmission amount by using the acquired new reference light transmission amount for storage.
Preferably, the reference light transmission amount is preset and stored before the analyzer leaves the factory.
Preferably, the light transmission amount is a reference light transmission amount of a suction head not placed at a suction head placing position obtained after the power-on and is stored.
The invention also provides a chemiluminescence detection device, which is used for judging the type of a sample sucked by a syringe loaded with a suction head, and comprises the following components:
the light transmission amount detection module is used for acquiring reference light transmission amount;
the control module is used for controlling the injector to load and lift the suction head;
the light transmittance detection module is also used for acquiring the lifting light transmittance of the placement position after the injector is loaded with the lifting suction head;
the calculation module is used for calculating the ratio of the lifting light transmission quantity to the reference light transmission quantity;
and the judging module is used for judging whether the suction head is loaded in the injector or not according to the calculated ratio.
Preferably, the light transmission amount detection module is further configured to obtain a new reference light transmission amount at any time when no tip is placed in the tip placement position, and update and store the stored reference light transmission amount with the new reference light transmission amount.
The present invention also provides a chemiluminescent immunoassay analyzer comprising:
a memory for storing a computer program;
a processor for invoking a computer program stored in the memory to implement the chemiluminescence detection method described above.
The present invention also provides a computer-readable storage medium storing a computer program which, when executed by a processor, implements the steps of the chemiluminescence detection method of the chemiluminescence immunoassay analyzer described above.
Compared with the prior art, the chemiluminescence detection method judges the type of the sample in the suction head (tip head) by adopting a ratio calculation mode, can better adapt to the attenuation of each component of an instrument, thereby reducing the requirement on a hardware board card, avoiding unstable detection result of the light transmittance of each channel due to factors such as self aging, differentiation or loading of a photoelectric module such as an LED light source or a photoelectric receiver, dynamically acquiring new reference light transmittance in real time to update and replace the stored reference light transmittance when the placing position of the tip head is empty, judging by dynamically updating the acquired reference light transmittance in real time when judging whether the tip head is placed or not, and whether an injector successfully loads the tip head and the sample type sucked by the tip head, thereby ensuring timely and accurate judgment of the loading condition of the tip head and corresponding prompt to ensure accurate and timely prompt of the loading state of the tip head, the reliability of the detection performance is further improved. The invention also provides a chemiluminescence detection device, a chemiluminescence immunoassay analyzer and a readable storage medium with the same technical effect.
Drawings
FIG. 1 is a flow chart of a chemiluminescence detection method of a chemiluminescence immunoassay analyzer provided by the present invention;
FIG. 2 is a block diagram of the structure of the chemiluminescence detection apparatus of the chemiluminescence immunoassay analyzer provided by the present invention.
FIG. 3 is a block diagram of the chemiluminescence immunoassay analyzer provided by the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in fig. 1, the present invention provides a method for determining a type of a sample, which is used for determining a type of a sample sucked by a tip-loaded syringe, wherein the method comprises the following steps:
s101, acquiring a reference light transmission amount;
specifically, the reference light transmittance is a light transmittance of the instrument when the tip head is not placed at the tip head placing position in the initial state, the reference light transmittance can be preset before the instrument leaves a factory, the instrument is started for the first time to automatically obtain the preset reference light transmittance, and the reference light transmittance can be generally set according to the performance and the working environment of related components of the instrument. The reference light transmission quantity can also be set before leaving a factory, the reference light transmission quantity of a tip head not placed in a tip head placing position is obtained immediately after the instrument is started for the first time and is used as the initial reference light transmission quantity to be stored, and then the tip head is placed and corresponding subsequent operation is carried out. In this embodiment, the reference light transmission amount is set and stored in advance before shipment.
It can be understood that the light transmittance may be a voltage value or a current value output after the light received by the light receiving module is converted into a corresponding electrical signal, and the light receiving module generally receives light from a tip placement position or a detection position.
S102, obtaining a placing light transmission quantity B0 of the tip head at the tip head placing position;
it can be understood that after the tip heads are placed at the tip head placement positions of each channel, the light transmission amount of each tip head is respectively obtained, and if six channels are provided, six tip heads are correspondingly placed, so that the light transmission amounts of the six tip heads are obtained.
S103, calculating the ratio of the placing light transmission quantity to the stored reference light transmission quantity and judging whether the tip head is placed according to the ratio;
in this embodiment, the ratio is calculated according to a preset formula B0/B, and whether the tip head is placed is determined according to the size of the ratio, where the preset formula B0 is the placing light transmission amount of the tip head, and B is a stored reference light transmission amount; if the judgment result is yes, namely the tip head is placed on the placing position, the step S104 is entered, and if the judgment result is no, namely the tip head is not placed on the placing position, an alarm prompt is needed to stop working at the moment. It will be appreciated that the predetermined formula may also be B/B0. It is understood that the ratio may also be a ratio obtained by performing corresponding mathematical transformation on the light transmission amount, for example, the ratios ln (B0)/ln (B), lg (B0)/lg (B), and logN (B0)/logN (B) obtained by respectively logarithmizing B0 and B, and the corresponding determination threshold may also be set accordingly. Since ln (B0)/ln (B), lg (B0)/lg (B), logN (B0)/logN (B) become larger, the corresponding threshold setting becomes larger.
It should be noted that, when the judgment is performed according to the size of the ratio, the calculated ratio is compared with a preset first threshold, in this embodiment, when the ratio B0/B is less than or equal to the preset first threshold, it is judged that the tip head is placed, and the next judgment on whether the tip head is loaded in the injector is performed, and when the ratio B0/B is greater than the first threshold, it indicates that the tip head is not placed in the tip head placement position.
It is understood that the size of the threshold is determined according to the tip head material of the instrument, the performance of the optical module, and the like, and preferably, the preset first threshold is in a range of 40% to 95%, in this embodiment, the first threshold is 85%.
In this embodiment, the following table shows the following steps of determining the respective states of tip heads corresponding to six channels according to the calculated transmittance (ratio of corresponding transmittance) in the chemiluminescence detection method:
as shown in the table, when tip heads are not placed on the placement positions, the light transmittance of each channel is 100%; after the tip head is placed, the light transmittance of each channel is smaller than 85% of a first threshold value, the tip head is judged to be placed in each channel, wherein the light transmittance of each channel after the tip head is placed in each channel is not completely the same, generally, a little deviation can occur due to the attenuation of an instrument element, the difference of a detection distance or the loading error of the tip head, but the deviation does not influence the judgment result compared with the first threshold value.
S104, controlling the injector to obtain the lifting light transmittance of the placing position after the lifting tip head is loaded;
after the tip head is placed in the tip head placing position, the injector is controlled to load the tip head on the placing position and move upwards to lift the tip head, the lifting light transmittance of the placing position at the moment is obtained when the injector loads the tip head and lifts the tip head to a light path which does not shield the optical module, it can be understood that after the injector successfully loads the tip head and lifts, the placing position does not have the tip head at the moment, the obtained reference light transmittance is also the reference light transmittance of the placing position, if the tip head is not successfully loaded, a channel 4 shown in a table does not successfully load the tip head, and the obtained light transmittance is the light transmittance for placing an empty tip head.
S105, calculating the ratio of the lifting light transmission quantity to the stored reference light transmission quantity and judging whether the tip head is loaded or not according to the ratio;
in this embodiment, the ratio is calculated according to a preset formula B1/B, and whether the tip head is loaded is determined according to the size of the ratio, where B1 is the light transmission amount for increasing; if the tip head is loaded in the placing position, namely the injector is judged to be loaded with the tip head, the step S106 is carried out, if the tip head is not loaded in the placing position, the situation that the tip head is loaded abnormally is judged, if the tip head is loaded askew or the tip head is not loaded on the injector, and at the moment, the analyzer needs to give an alarm to prompt and stop working; it will be appreciated that the predetermined formula may also be B/B1.
It should be noted that, the ratio B1/B is compared with a preset first threshold, when the ratio B1/B is greater than the preset first threshold, it is determined that the tip head is successfully loaded by the injector, and when the ratio B1/B is less than or equal to the first threshold, it is determined that the tip head is abnormally loaded.
S106, controlling the injector to load the tip head to move to a detection position and obtaining the light transmittance of the tip head at the detection position;
specifically, the injector moves a certain distance to a detection position of the instrument after loading the tip head, in the moving process of the injector, if the tip head loaded in a certain channel falls, in the light transmittance of the tip head detection position obtained in the detection position, the light transmittance of the injector with the dropped tip head is the reference light transmittance without the tip head, the tip head loaded in the injector of the channel 1 is displayed in table 1 to fall, and the light transmittance is 100%.
S107, calculating the ratio of the light transmission quantity of the detection position to the stored reference light transmission quantity, and judging whether a tip head is loaded on the detection position according to the size of the ratio;
in the embodiment, the ratio is calculated according to a preset formula B2/B, and whether the tip head is loaded is judged according to the size of the ratio, wherein B2 is the detection position light transmission amount of the tip head; if so, judging that the tip head loaded on the injector at the detection position does not fall off, and entering step S108, otherwise, judging that the tip head loaded on the injector falls off when moving from the placement position to the detection position, namely, the tip head is not loaded on the injector at the detection position, and at the moment, alarming and prompting the instrument to stop working; it will be appreciated that the predetermined formula may also be B/B2.
In the present embodiment, the threshold range set for the determination of the magnitude of the contrast value B2/B is: the second threshold is not more than B2/B is not more than a third threshold, the value range of the second threshold is 0-10%, the value range of the third threshold is 60-90%, preferably the second threshold is 10%, and the third threshold is 85%. Comparing the ratio B2/B with a preset threshold, judging that the injector successfully loads the tip head when the ratio B2/B is within a range defined by a second threshold and a third threshold, moving the injector loaded with the tip head to a sample sucking position for sucking samples, and judging that abnormality occurs such as aging fault abnormality of components when the ratio B2/B is smaller than the second threshold; and when the B2/B is larger than a third threshold value, judging that the tip head is loaded and dropped, namely the tip head is not loaded when the injector is at the detection position.
If the tip head of a certain channel injector is not successfully loaded at the placing position or the tip head falls off in the process that the injector moves to the detection position after being loaded from the placing position, the table shows that when the channel 4 for loading the tip head at the placing position is not successfully loaded and lifted, the tip head is still placed on the channel 4, so that the light transmittance of the channel 4 is approximately the same as that of the tip head, at the moment, due to the measurement deviation of a component and the updating of the light transmittance of a vacant position in a calculation formula, the light transmittance of the channel 4 for not loading the tip head is approximately smaller than that of a first placing position for placing the tip head, at the moment, the light transmittance of the channel 4 injector for not loading the tip head is smaller than a first threshold value, and the situation that the channel 4 is not successfully loaded with the tip head at the placing position is judged. After the injector with the tip head moves to the detection position from the placement position, if the tip head loaded by the injector of the channel 1 falls in the moving process, when the light transmission quantity on the channel 1 is obtained on the detection position, the light transmittance is 100% due to no tip head shielding, namely the light transmittance is larger than a third threshold value, the fact that the tip head is not loaded in the detection position of the channel 1 is shown, and the tip head falls from the injector in the process of moving to the detection position after being loaded and lifted from the placement position.
S108, obtaining the sample sucking light transmittance B3 of the sample sucking tip head after the loading tip head moves to the sample sucking position for sample sucking;
specifically, the tip head loaded on the injector is controlled to bind the reagent strip and then move to a sample sucking position, the tip head sucks the sample at the sample sucking position and moves to a detection position after the sample sucking to obtain the sample sucking light transmittance B3 of the tip head at the detection position.
And S109, judging the type of the sample in the tip head according to the sample sucking light transmission amount and the stored reference light transmission amount.
The sample type can be generally determined according to the magnitude relationship between the sample absorption light transmission amount and the reference light transmission amount, in this embodiment, the ratio is calculated according to a preset formula B3/B, and the sample type is determined according to the magnitude of the ratio, where B3 is the sample absorption light transmission amount of the obtained tip, and B is the stored reference light transmission amount. The general blood sample types are divided into whole blood or serum, the ratio B3/B is compared with a preset fourth threshold and a preset fifth threshold, the whole blood is obtained when the ratio B3/B is smaller than the preset fourth threshold, the serum is obtained when the ratio B3/B is larger than the preset fifth threshold, and if the ratio B3/B is between the fourth threshold and the fifth threshold, the tip head is judged to be abnormal in sample suction, such as empty tip heads or insufficient sample suction amount of the tip heads, and at the moment, the analyzer needs to give an alarm to prompt to stop working; it will be appreciated that the predetermined formula may also be B/B3.
It should be noted that the fourth threshold is smaller than the fifth threshold, the range of the preset fourth threshold is 10% -20%, the range of the preset fifth threshold is 30% -45%, in this embodiment, the fourth threshold is 15%, and the fifth threshold is 37%. As shown in the above table, the light transmittance of each channel is less than 15% of the fourth threshold, the sample is whole blood, the light transmittance is relatively low, the light transmittance of each channel is greater than 37% of the fifth threshold, the sample is serum, and the light transmittance is relatively high.
In the chemiluminescence detection step of judging whether a tip head is placed or not, whether the tip head is loaded or not and whether the tip head is sucked or not, if the stored reference light transmission amount B is required to be obtained, the reference light transmission amount B can not be kept constant due to aging of components and the like at different moments, if the light transmission amount is preset in a factory, the reference light transmission amount in the actual repeated detection process is different from that set in the factory, even if the reference light transmission amount is automatically measured and stored after the power is turned on, the reference light transmission amount can be dynamically updated as required in the use process of the instrument along with different aging degrees of the components in the detection use process of the instrument, the reference light transmission amount is different from that in the initial state, in order to ensure the detection accuracy of the instrument, the reference light transmission amount B 'can be dynamically updated as required in the use process of the instrument, and the stored reference light transmission amount B is stored after the stored reference light transmission amount B is updated by using the obtained new reference light transmission amount B' And is used as the reference light transmission amount when the ratio needs to be calculated next time.
Due to aging of components of the instrument, when the value of the new reference light transmittance B 'obtained dynamically in real time is smaller than a threshold value, the obtained value of the reference light transmittance B' is invalid, the instrument can give an alarm to prompt abnormity and stop working, and a user needs to replace the LED light source or related components thereof according to the actual condition of the instrument, so that the accuracy of the instrument is ensured, and the working efficiency of the instrument is improved. In the present embodiment, the threshold is set to 50% of the initially acquired reference light transmission amount B, and it is understood that the threshold may be set as needed.
In this embodiment, after the step S104 of loading the lift tip head, the reference light transmittance of the placement position is dynamically obtained and updated and stored, and the reference light transmittance in the subsequent calculation and determination process is the new reference light transmittance, that is, the reference light transmittance in the calculation formula in the steps S107 and S109 is the updated new reference light transmittance.
If the injector is controlled to load the tip head and move away from the tip head placement position in step S106, and the tip head placement position is empty, a new reference light transmission amount B 'of the tip placement position may be obtained at this time, and the newly obtained reference light transmission amount B' is updated to replace the previously obtained and stored reference light transmission amount B. In the calculation and judgment steps of steps S107 and S109, the ratio is calculated using the stored new reference light transmission amount B'.
Similarly, if the tip head is moved to the sample suction position after the injector is controlled to load the tip head, and the tip head is not placed in the tip head placement position, the reference light transmission amount B 'of the tip placement position can be obtained at this time, and the newly obtained reference light transmission amount B' is updated to replace the previously obtained and stored reference light transmission amount B, and in the calculation and judgment step of step S109, the ratio B3/B 'is calculated by using the stored new reference light transmission amount B'.
Similarly, if the initial reference light transmittance is set before the instrument leaves the factory, the reference light transmittance of the tip head placement position where the tip head is not placed can be immediately and automatically measured and obtained after the instrument is started to serve as a new reference light transmittance B ', and the newly obtained reference light transmittance B' is updated to replace the initial reference light transmittance set after the instrument leaves the factory so as to complete dynamic updating of the reference light transmittance, that is, the dynamic updating of the reference light transmittance can be started after the instrument is started, so that the accuracy of the instrument detection and the chemiluminescence detection is further improved.
And as long as the placing position of the tip head is empty, the new reference light transmission amount can be dynamically obtained in real time to update and replace the stored reference light transmission amount, and the reference light transmission amount in each calculation step is ensured to be the reference light transmission amount obtained by dynamic real-time updating, so that the judgment of the sample type is more accurate. And after the sample type is judged, the analyzer correspondingly detects the sample according to the judged sample type in each tip head and outputs a corresponding detection result.
Referring to fig. 2, the present invention further provides a chemiluminescence detection apparatus for implementing the above-described chemiluminescence detection method, wherein the chemiluminescence detection apparatus comprises a control module 100, a light transmittance detection module 200, a storage module 300, a calculation module 400, and a determination module 600.
In this embodiment, the light transmittance detection module 200 is configured to obtain a reference light transmittance from a factory preset storage in the storage module 300, and it can be understood that, if the reference light transmittance is not set before factory, the light transmittance detection module 200 needs to obtain, immediately after the analyzer is turned on, a light transmittance when a tip head is not placed in a tip head placement position as an initial reference light transmittance B and store the initial reference light transmittance B in the storage module 300.
After the tip head is placed, the light transmittance detection module 200 obtains the placing light transmittance of the tip head at the tip head placing position; after the control module 100 controls the injector to load the lift tip head, and simultaneously controls the light transmittance detection module 200 to obtain the lift light transmittance at the placement position; after the control module 100 controls the injector to load the tip head to move to the detection position, the light transmittance detection module 200 obtains the light transmittance of the tip head at the detection position; the control module 100 obtains the sample suction light transmittance of the tip head after controlling the injector loading tip head to move to the sample suction position for sample suction.
The calculating module 400 is configured to calculate ratios of a placement light transmission amount, a lifting light transmission amount, a detection position light transmission amount, a sample sucking light transmission amount, and a stored reference light transmission amount, respectively, and the determining module 600 is configured to determine a placement state and a loading state of a tip head and a type of a sample sucked by the tip head according to the ratios and a preset corresponding threshold, and if the determining module 600 determines whether the tip head is placed according to the calculated ratio of the placement light transmission amount to the stored reference light transmission amount and a preset first threshold.
It can be understood that, when the stored reference light transmission amount in the storage module 300 needs to be updated, at any time when the tip head is not placed in the tip head placement position after the syringe is removed from the tip head placement position for loading the tip head, the control module 100 controls the light transmission amount detection module 200 to obtain a new reference light transmission amount of the placement position and simultaneously replace the stored reference light transmission amount B with the obtained new reference light transmission amount and store the new reference light transmission amount in the storage module 300. If the tip head is loaded and moved away from the tip head placement position by the injector, the tip head placement position is empty at this time, a new reference light transmission amount B ' of the tip placement position can be obtained at this time, and at this time, the newly obtained reference light transmission amount B ' needs to be updated to replace the previously stored reference light transmission amount B, that is, the new reference light transmission amount B ' stored in the storage module 300, and the calculation module 400 calculates the ratio according to the preset formula, and then the calculation module 400 needs to calculate the ratio by using the new reference light transmission amount B ' stored in the storage module 300, and if the judgment module judges the sample type according to the calculated ratio of the sample absorption light transmission amount B3 to the new reference light transmission amount B '.
The chemiluminescence detection apparatus of this embodiment is used to implement the chemiluminescence detection method of the chemiluminescence immunoassay analyzer, and thus the specific implementation manner of the chemiluminescence detection apparatus of the chemiluminescence immunoassay analyzer can be found in the embodiment portion of the chemiluminescence detection method of the chemiluminescence immunoassay analyzer in the foregoing, for example, the light transmittance detection module 200 is used to implement steps S101, S102, S104, S106, and S108 in the chemiluminescence detection method of the chemiluminescence immunoassay analyzer, and the calculation module 400 and the determination module 600 are used to implement steps S103, S105, S107, and S109 in the chemiluminescence detection method of the chemiluminescence immunoassay analyzer, so the specific implementation manner thereof can refer to the description of the corresponding embodiment, and will not be described herein again.
The chemiluminescence detection device provided by the invention can better adapt to the attenuation of each component of an instrument by adopting the calculation of the ratio, thereby reducing the requirement on a hardware board card, avoiding the instability of the detection result of the light transmittance of each channel due to the aging, differentiation or loading of photoelectric modules such as LED light sources or photoelectric receivers, dynamically obtaining new reference light transmittance in real time to update and replace the stored reference light transmittance when the placement position of the tip head is empty, and further utilizing the obtained reference light transmittance to update and judge in real time when the injector is judged whether to successfully load the tip head in steps S102-S107 and the type of a sample sucked by the tip head is judged in steps S108-S109, thereby further improving the reliability of the detection performance.
The present invention also provides a chemiluminescence immunoassay analyzer, and fig. 3 is a block diagram of a chemiluminescence immunoassay analyzer provided in an embodiment of the present invention, and the chemiluminescence immunoassay analyzer may include:
a memory 1 for storing a computer program;
a processor 2 for invoking a computer program stored in said memory for implementing the steps of the method of chemiluminescence detection of a chemiluminescence immunoassay analyzer as described above.
Further, the chemiluminescence immunoassay analyzer provided by the embodiment may further include:
and the display 3 is used for displaying a human-computer interaction interface for receiving input instructions.
It can be understood that the functions of the functional modules of the chemiluminescence immunoassay analyzer of this embodiment can be specifically implemented according to the method in the above method embodiment, and the specific implementation process thereof can refer to the related description of the above method embodiment, which is not described herein again.
The chemiluminescence immunoassay analyzer provided by the invention can better adapt to the attenuation of each component of the analyzer by adopting the calculation of the ratio, thereby reducing the requirement on a hardware board card, avoiding the instability of the detection result of the light transmittance of each channel due to the aging, differentiation or loading of photoelectric modules such as LED light sources or photoelectric receivers, dynamically obtaining new reference light transmittance in real time to update and replace the stored reference light transmittance when the placing position of the tip head is empty, and further utilizing the dynamic real-time updating of the obtained reference light transmittance to judge whether the tip head is placed or not, whether the injector successfully loads the tip head and the type of a sample sucked by the tip head, thereby further improving the reliability of the detection performance.
In addition, the present invention also provides a readable storage medium having stored thereon a computer program which, when executed by a processor, implements the steps of the chemiluminescence detection method of the above chemiluminescence immunoassay analyzer.
It is to be understood that, when being executed by a processor, the readable storage medium of this embodiment may be specifically implemented according to the method in the foregoing method embodiment, and a specific implementation process of the readable storage medium may refer to the description related to the foregoing method embodiment, which is not described herein again.
The embodiments are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same or similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
Those of skill would further appreciate that the various illustrative elements and algorithm steps described in connection with the embodiments disclosed herein may be implemented as electronic hardware, computer software, or combinations of both, and that the various illustrative components and steps have been described above generally in terms of their functionality in order to clearly illustrate this interchangeability of hardware and software. Whether such functionality is implemented as hardware or software depends upon the particular application and design constraints imposed on the implementation. Skilled artisans may implement the described functionality in varying ways for each particular application, but such implementation decisions should not be interpreted as causing a departure from the scope of the present invention.
The steps of a method or algorithm described in connection with the embodiments disclosed herein may be embodied directly in hardware, in a software module executed by a processor, or in a combination of the two. A software module may reside in Random Access Memory (RAM), memory, Read Only Memory (ROM), electrically programmable ROM, electrically erasable programmable ROM, registers, hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art.
The chemiluminescence immunoassay method and device, chemiluminescence immunoassay instrument and readable storage medium provided by the invention are described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (8)
1. A method of chemiluminescent detection comprising:
acquiring a reference light transmission amount;
controlling the injector loading suction head to move to a detection position and acquiring the light transmittance of the suction head at the detection position;
calculating the ratio of the light transmission quantity of the detection position to the stored reference light transmission quantity and judging whether the suction head is loaded at the detection position according to the size of the ratio;
before judging that the suction head is loaded at the detection position, the method also comprises the following steps:
acquiring the placing light transmission quantity of the placed suction head at the placing position of the suction head;
calculating the ratio of the placed light transmission quantity to the stored reference light transmission quantity and judging whether the sucker is placed according to the ratio;
before judging that the suction head is loaded at the detection position, the method also comprises the following steps:
controlling the injector to obtain the lifting light transmittance of the placing position after loading the lifting sucker;
and calculating the ratio of the lifting light transmission quantity to the stored reference light transmission quantity and judging whether the sucker is loaded according to the ratio.
2. The chemiluminescent detection method of claim 1 further comprising the steps of: and acquiring a new reference light transmission amount at any time when no suction head is placed at the placing position of the suction head, and updating and replacing the stored reference light transmission amount by using the acquired new reference light transmission amount for storage.
3. The chemiluminescence detection method according to claim 1, wherein the reference transmission amount is preset and stored before shipment of the analyzer.
4. The method for detecting chemiluminescence according to claim 1, wherein the light transmittance is a reference light transmittance obtained after start-up when no tip is placed at a tip placement position and stored.
5. A chemiluminescence detection device for judging the type of a sample sucked by a syringe loaded with a suction head, which is characterized by comprising:
the light transmission amount detection module is used for acquiring reference light transmission amount;
the control module is used for controlling the injector to load and lift the suction head;
the light transmittance detection module is also used for acquiring the light transmittance of the detection position after the injector is loaded with the lifting suction head;
the calculation module is used for calculating the ratio of the light transmission quantity of the detection position to the reference light transmission quantity;
the judging module is used for judging whether the suction head is loaded on the injector according to the calculated ratio;
before judging that detects the position and load the suction head still include:
acquiring the placing light transmission quantity of the placed suction head at the placing position of the suction head;
calculating the ratio of the placed light transmission quantity to the stored reference light transmission quantity and judging whether the sucker is placed according to the ratio;
before judging that detects the position and load the suction head still include:
controlling the injector to obtain the lifting light transmittance of the placing position after loading the lifting sucker;
and calculating the ratio of the lifting light transmission quantity to the stored reference light transmission quantity and judging whether the sucker is loaded according to the ratio.
6. The chemiluminescence detection apparatus according to claim 5, wherein the light transmittance detection module is further configured to obtain a new reference light transmittance at any time when the tip is not placed at the tip placement position and to update and store the stored reference light transmittance with the new reference light transmittance.
7. A chemiluminescent immunoassay analyzer, comprising:
a memory for storing a computer program;
a processor for invoking a computer program stored in the memory to implement the chemiluminescence detection method of any one of claims 1-4.
8. A readable storage medium, characterized in that the readable storage medium stores a computer program which, when executed by a processor, implements the steps of the chemiluminescence detection method according to any one of claims 1 to 4.
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