CN108977344A - A kind of separating extraction device and separating and extracting process - Google Patents
A kind of separating extraction device and separating and extracting process Download PDFInfo
- Publication number
- CN108977344A CN108977344A CN201811110374.3A CN201811110374A CN108977344A CN 108977344 A CN108977344 A CN 108977344A CN 201811110374 A CN201811110374 A CN 201811110374A CN 108977344 A CN108977344 A CN 108977344A
- Authority
- CN
- China
- Prior art keywords
- cabin
- liquid
- fixator
- gun head
- magnet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of separating extraction device and separating and extracting process, the separating extraction device includes: pedestal, and the support construction being set on the base, it is provided with moving assembly on the support structure, moving assembly includes fixator, fixator includes adapter, adapter is detachably connected with liquid transfer gun head, fixator is connected to by adapter with the channel being arranged in liquid transfer gun head, fixator is connect with the pump housing by conduit, liquid transfer gun head is set to suck or be discharged liquid, sliding rail is additionally provided on pedestal, sliding rail is slidably connected with fixed frame, kit is provided in fixed frame, kit includes holding liquid tank and waste liquid cabin, it holds liquid tank and useless tank roof is equipped with opening, Ka Gai is equipped in opening, elaxtic seal is provided between lid being open and blocking, magnet is additionally provided on fixed frame, magnet can be detachably connected with adapter.The device streamline operation, promotion can separate precision in separation and Extraction biomolecular processes.
Description
Technical field
The present invention relates to molecular diagnostic techniques fields, in particular to a kind of separating extraction device and separation and Extraction side
Method.
Background technique
In molecular biology and in-vitro diagnosis field, no matter scientific research demand or clinical diagnosis, be both needed to by quantitative or fixed
Property detection sample in specific biomolecule, such as: specific nucleic acid sequence or certain protein, come carry out biological information inverting and
Medical diagnosis on disease.It under these application demands, acquires and the biological sample that measures often complicated component, the life for needing to detect in addition
Object molecular concentration is often very low, and directly detection is more difficult.
At this stage generally using nanometer magnetic bead method come the scheme of separation and Extraction biomolecule, the program is needed using bar magnet,
Stirring set and corresponding reagent.
But the nanometer magnetic bead for being adsorbed with target biological molecules is repeatedly passed through bar magnet and stirring set lift-off liquid by whole process
Face exposure in air, the possibility for forming aerosol is drifted into air there are target biological molecules, this possibility is to laboratory
There is biological risk in personnel, while can also interfere with subsequent detection.
Summary of the invention
It is an object of the present invention in view of the deficiency of the prior art, a kind of separating extraction device and separation are provided
Extracting method streamline operation, promotion separation precision can make to purify liquid in separation and Extraction biomolecular processes
Purity it is higher.
To achieve the above object, technical solution used in the embodiment of the present invention is as follows:
In a first aspect, the embodiment of the invention provides a kind of separating extraction devices, comprising: exist including pedestal, and setting
Support construction on the pedestal is provided with moving assembly in the support construction, and the moving assembly includes fixator, institute
Stating fixator includes adapter, the adapter and liquid transfer gun head and punctures component and is detachably connected, and the fixator passes through institute
It states adapter and is connected to the channel being arranged in the liquid transfer gun head, the fixator is connect with the pump housing by conduit, and the shifting is made
The sucking of liquid pipette tips or discharge liquid, are additionally provided with sliding rail, the sliding rail is slidably connected with fixed frame, described on the base
Kit is provided in fixed frame, the kit includes holding liquid tank and waste liquid cabin, described to hold liquid tank and the useless tank roof
It is equipped with opening, is equipped with Ka Gai over said opening, is provided with elaxtic seal between the opening and card lid,
Magnet is additionally provided on the fixed frame, the magnet can be detachably connected with the adapter.
In a kind of embodiment, the moving assembly further includes Single Mechanical arm, and the Single Mechanical arm and the fixator are solid
Fixed connection.
It further include XYZ dynamical system in a kind of embodiment, the XYZ dynamical system is for controlling the fixed frame point
It is not mobile in Z-direction in X-direction, the movement of Y direction and the control moving assembly.
In a kind of embodiment, the kit further includes reagent storage cabin, first places cabin and second and place cabin, described
Card, which covers to be provided with, holds corresponding two through-holes of liquid tank and the waste liquid cabin with described respectively, it is described hold be provided on liquid tank it is removable
The first protective film unloaded；
The reagent storage cabin includes multiple reagent storage chambers, and the opening of the waste liquid cabin and the reagent storage chamber is solid
Equipped with the second protective film；
The first placement cabin and second placement cabin place liquid transfer gun head respectively and puncture component, and described first places
Dismountable third protective film is equipped at the top of cabin and second placement cabin.
In a kind of embodiment, the elaxtic seal includes at least one of silica gel, rubber.
In a kind of embodiment, positioning mark corresponding with the through-hole is provided on the elaxtic seal.
In a kind of embodiment, the two sides in the reagent storage cabin are respectively set that there are two fixed ears.
In a kind of embodiment, the liquid transfer gun head is disposable liquid transfer gun head, and the component that punctures is disposable aspiration needle.
In a kind of embodiment, the magnet is magnet or electromagnet.
Second aspect, the embodiment of the invention also provides a kind of separating and extracting process, which comprises
Separating extraction device according to claim 1 is provided；
Fixator carries out automatic load and punctures component, punctures component and punctures kit one by one；
Fixator load liquid transfer gun head shifts default reagent and enters to hold liquid tank, and loads magnet and carry out magnetic to biomolecule,
And it shifts waste liquid and enters waste liquid cabin；
Wherein, default reagent includes lytic reagent, washing reagent and elution reagent.
The beneficial effects of the present invention are:
A kind of separating extraction device and separating and extracting process provided in an embodiment of the present invention, by holding liquid tank and waste liquid cabin
Open top be provided with elaxtic seal so that liquid to be purified is constantly in sealed environment, realizes and transported in equipment
Liquid during work is not in leakage and contamination phenomenon, and by setting fixator, which has and can load automatically
With self-discharging function, fixator automatic load and unloading liquid transfer gun head, magnet respectively are realized, in addition, magnet setting exists
It holds on the outside of liquid tank, magnet is not limited by tank dimension is held, and volume is big, and magnetic bead active area in liquid tank is big with holding, magnetic chamber, time-consuming
Short, effect is good.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is separating extraction device overall structure diagram provided in an embodiment of the present invention；
Fig. 2 is kit overall structure diagram provided in an embodiment of the present invention；
Fig. 3 is kit side view provided in an embodiment of the present invention；
Fig. 4 is top view provided in an embodiment of the present invention；
Fig. 5 is magnet course of work schematic diagram provided in an embodiment of the present invention；
Fig. 6 is the course of work module map of separating extraction device provided in an embodiment of the present invention.
Icon: 1- separating extraction device；10- holds liquid tank；11- elaxtic seal；12- positioning mark；13- Ka Gai；15- is logical
Hole；The first protective film of 17-；20- waste liquid cabin；The second protective film of 21-；30- reagent storage cabin；31- fixed ear；40- first is placed
Cabin；41- liquid transfer gun head；43- third protective film；50- second places cabin；60- pedestal；70- support construction；80- moving assembly；
81- Single Mechanical arm；83- fixator；84- adapter；90- fixed frame；100- magnet；101- magnet magnetic position；103- magnet is empty
Not busy position；120- kit；The first dynamical system of 121-；The second dynamical system of 123-；125- third dynamical system；The 130- pump housing；
140- conduit；121a- first motor；The first sliding rail of 121b-；The second motor of 123a-；The second sliding rail of 123b-；125a- third electricity
Machine；125b- third sliding rail.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented
The component of example can be arranged and be designed with a variety of different configurations.
Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below claimed
The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
In the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, or be somebody's turn to do
Invention product using when the orientation or positional relationship usually put, be merely for convenience of description of the present invention and simplification of the description, without
It is that the device of indication or suggestion meaning or element must have a particular orientation, be constructed and operated in a specific orientation, therefore not
It can be interpreted as limitation of the present invention.In addition, term " first ", " second ", " third " etc. are only used for distinguishing description, and cannot manage
Solution is indication or suggestion relative importance.
In addition, the terms such as term "horizontal", "vertical" are not offered as requiring component abswolute level or pendency, but can be slightly
Low dip.It is not to indicate that the structure has been had to if "horizontal" only refers to that its direction is more horizontal with respect to for "vertical"
It is complete horizontal, but can be slightly tilted.
In the description of the present invention, it is also necessary to which explanation is unless specifically defined or limited otherwise, term " setting ",
" installation ", " connected ", " connection " shall be understood in a broad sense, for example, it may be fixedly connected, may be a detachable connection or one
Connect to body；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, it can also be indirect by intermediary
It is connected, can be the connection inside two elements.For the ordinary skill in the art, on being understood with concrete condition
State the concrete meaning of term in the present invention.
First embodiment
Fig. 1 is please referred to, the embodiment of the present invention provides a kind of separating extraction device 1, including pedestal 60, and is arranged described
Support construction 70 on pedestal 60 is provided with moving assembly 80 in support construction 70, and moving assembly 80 includes fixator 83, Gu
Determining device 83 includes adapter 84, and adapter 84 is detachably connected with liquid transfer gun head 41, and fixator 83 passes through adapter 84 and liquid relief
The channel connection being arranged in pipette tips 41, fixator 83 are connect with the pump housing 130 by conduit 140, and the sucking of liquid transfer gun head 41 or row are made
Liquid out is additionally provided with the first sliding rail 121b on pedestal 60, and the first sliding rail 121b is slidably connected with fixed frame 90, in fixed frame
Kit 120 is provided in 90；
Referring to figure 2., kit 120 includes holding liquid tank 10 and waste liquid cabin 20, holds and is all provided at the top of liquid tank 10 and waste liquid cabin 20
There is opening, card lid 13 is equipped in opening, elaxtic seal 11 is provided between opening and card lid 13, on fixed frame 90
It is additionally provided with magnet 100, magnet 100 can be detachably connected with adapter 84.
In the present embodiment, the adapter 84 being arranged on fixator 83 can be round boss, set on liquid transfer gun head 41
Circular groove, fixator 83 and liquid transfer gun head 41 is equipped with to load and unload automatically by the round boss and the circular groove, it should
Adapter 84 can be magnetically attractive stick, be provided with through-hole on magnet 100, protrudes into the through-hole by the Magnetic suction rod, passes through mutual magnetic
The magnet 100 is fixed with fixator 83 in power.
In the present embodiment, liquid tank 10 and waste liquid cabin 20 are held and is equipped with concordant opening, therefore, entirety can be passed through
Elaxtic seal 11 and Ka Gai 13 seal the opening for holding liquid tank 10 Yu waste liquid cabin 20 together, can play save material in this way
Effect, the uneven opening of height can also be equipped with by holding liquid tank 10 and waste liquid cabin 20, be blocked by two elaxtic seals 11 and two
Lid 13 seals the opening for holding liquid tank 10 Yu waste liquid cabin 20 respectively, can to hold liquid tank 10, waste liquid cabin 20 in this way at any one
Sealing when something goes wrong, facilitates replacement to repair.It holds as liquid tank 10 can be set into the cabin length in waste liquid cabin 20, it can also
It is different to be arranged to, it can specifically be arranged according to the actual situation, in the present embodiment, the length for holding liquid tank 10 compares waste liquid
The length in cabin 20 is short, and the length in waste liquid cabin 20 is the waste liquid cabin in order to guarantee during separating-purifying greater than 10 length of liquid tank is held
20 have enough spaces to place waste liquid.
A kind of separating extraction device provided in an embodiment of the present invention, by being arranged in the open top for holding liquid tank and waste liquid cabin
Flexible sealing material realizes the liquid in equipment operation so that liquid to be purified is constantly in sealed environment
It is not in leakage and contamination phenomenon, and by setting fixator, which has and can load automatically and self-discharging function
Can, fixator automatic load and unloading liquid transfer gun head, magnet respectively are realized, in addition, magnet setting is being held on the outside of liquid tank, magnet
It is not limited by tank dimension is held, volume is big, and magnetic bead active area in liquid tank is big with holding, magnetic chamber, and time-consuming short, effect is good.
Fig. 1 is please referred to, in the present embodiment, moving assembly 80 further includes Single Mechanical arm 81, Single Mechanical arm 81 and fixator
83 are fixedly connected, and are provided with fluid flow paths in the Single Mechanical arm 81, and one end of the fluid flow paths and conduit 140 connect
Logical, the other end is connected to fixator 83, and the liquid transfer gun head 41 that can be used to control the installation of fixator 83 by using the pump housing 130 is right
Liquid is sucked or is discharged.
Fig. 1 is please referred to, in the present embodiment, which further includes XYZ dynamical system, the XYZ dynamical system
It unites for controlling the fixed frame 90 respectively in X-direction, the movement of Y direction and the control moving assembly 80 in Z axis
Direction is mobile.
Wherein, XYZ dynamical system includes the first dynamical system 121, the second dynamical system 123 and third dynamical system 125,
The XYZ dynamical system primarily to facilitate operation moving assembly 80 and kit 120 with the use of process, three can be provided
The power in a direction, wherein the first dynamical system 121 can provide the power of Y direction, drive kit 120 along the y axis
It moving left and right, the second dynamical system 123 can provide the power of X-direction, and drive kit 120 to be moved forward and backward along the x axis,
Third dynamical system 125 can provide the power of Z-direction, and moving assembly 80 is driven to move up and down along Z-direction, so that
Experimenter can easily carry out purification operations.
Further, in the present embodiment, the first dynamical system 121 includes first motor 121a and the first sliding rail 121b,
Fixed frame 90 is movably set on first motor 121a, fixed frame 90 is slidably connected with the first sliding rail 121b；Second dynamical system
123 include being movably set with fixed frame 90 on the second motor 123a and the second sliding rail 123b, the second motor 123a, fixed frame 90 with
Second sliding rail 123b is slidably connected；Third dynamical system 125 includes third motor 125a and third sliding rail 125b, third motor
It is movably arranged on 125a on Single Mechanical arm 81, Single Mechanical arm 81 is slidably connected with third sliding rail 125b.
Specifically, first motor 121a control fixed frame 90 is slided in the first sliding rail 121b, the second motor 123a control is solid
Determine frame 90 to slide on the second sliding rail 123b, third motor 125a control Single Mechanical arm 81 slides on third sliding rail 125b.
Optionally, which is not limited to arrangement above mode, which can relatively move in spatial position, only
Want to guarantee that kit 120 and moving assembly 80 on fixed frame 90 can cooperate.
Referring to figure 2., in the present embodiment, kit 120 further includes that cabin 40 and second is placed in reagent storage cabin 30, first
Place cabin 50；Be provided on card lid 13 respectively with hold corresponding two through-holes 15 of liquid tank 10 and waste liquid cabin 20, hold and set on liquid tank 10
It is equipped with dismountable first protective film 17；Reagent storage cabin 30 includes multiple reagent storage cabins, the opening of multiple reagent storage chambers
Concordantly, the opening in waste liquid cabin 20 and reagent storage cabin 30 is installed with the second protective film 21；First, which places cabin 40 and second, places
Cabin 50 places liquid transfer gun head 41 respectively and punctures component, and the top in the first placement cabin 40 and the second placement cabin 50 is equipped with detachably
Third protective film 43.
Referring to figure 2. and Fig. 3 holds liquid tank 10, waste liquid cabin 20, the placement cabin of reagent storage cabin 30, first in the present embodiment
40 and second placement cabin 50 can be integrally formed, reagent storage cabin 30 include multiple reagent storage chambers, multiple reagent storage chambers
Cavity length can be set into identical, and how many with specific reference to the amount for needing to place reagent and set, multiple reagent storage chambers can be with
Identical reagent is placed, different reagents can also be placed, can be selected according to the reagent that separating-purifying needs.
The quantity in the first placement cabin 40 and the second placement cabin 50 can be set according to the actual situation, in the present embodiment
In, first, which places cabin 40, includes two placement cabins, and second, which places cabin 50, includes a placement cabin, and the first placement cabin 40 is for placing
Disposable liquid transfer gun head 41, the second placement cabin 50 puncture component for placing, and optionally, the first placement cabin 40 may include multiple the
One places cabin, and multiple placement cabins are placed with disposable liquid transfer gun head 41.
Referring to figure 2., in the present embodiment, it is put with second in the first protective film 17, the first placement cabin 40 held on liquid tank 10
The top for setting cabin 50, which is equipped with the purpose that dismountable third protective film 43 is detachably arranged, allows operator in operation
When can be convenient and open the first protective film 17 and third protective film 43 manually, the second protective film 21 on reagent storage cabin 30 is solid
Surely the purpose being arranged is to guarantee that the reagent inside reagent storage cabin 30 is not contaminated.
Fig. 1 is please referred to, in the present embodiment, elaxtic seal 11 includes at least one of silica gel, rubber, and elasticity is close
Closure material 11 is used to that liquid tank 10 will to be held and waste liquid cabin 20 seals, so that the detection reduced to liquid to be purified is interfered.
It is provided on elaxtic seal 11 corresponding with the through-hole 15 in the present embodiment with Fig. 4 referring to figure 2.
Positioning mark 12, which can be cross, be also possible to the central coordinate of circle of through-hole, can also be the seat with graduation mark
Mark is arranged the purpose of the positioning mark 12 in order to facilitate liquid transfer gun head 41 and holds liquid tank 10 and waste liquid cabin 20 with component positioning is punctured
Position.
Referring to Fig.1 and 2, in the present embodiment, the two sides in reagent storage cabin 30 are respectively arranged with fixed ear 31, this is solid
Ear 31 is determined for kit 120 and fixed frame 90 is fixed.
Fig. 1 is please referred to, in the present embodiment, liquid transfer gun head 41 is disposable liquid transfer gun head, and puncturing component is disposable needle
It manages, the pipeline of connecting tube 140 is provided on the disposable liquid transfer gun head, because separating-purifying is to the degree of purity of liquid to be purified
It is required that it is very high, therefore, for liquid transfer gun head 41 and component is punctured, is all made of disposably, to avoid superinfection, caused inspection
The interference of survey.
Fig. 1 is please referred to, in the present embodiment, the side in fixed frame 90 is placed with magnet 100, another in fixed frame 90
Side is isolated with magnet 100 is provided with kit 120, and the side in fixed frame 90 is placed with magnet 100, places the one of magnet 100
Side can be magnet spare bits 103, and the other side in fixed frame 90 and magnet spare bits 103 are relatively set with magnet magnetic
Position 101, the magnet magnetic position 101 hold that liquid tank 10 is adjacent, and when operating without magnetic, which is to be in this
Magnet spare bits 103, when if necessary to carry out magnetic operation, the magnet 100 can be placed by moving assembly 80
At magnet magnetic position 101, for carrying out magnetic to liquid to be purified.
Referring to figure 5., in the present embodiment, magnet 100 includes magnet or electromagnet, and the magnet 100 in separation for mentioning
Magnetic operation carried out to the liquid that holds in liquid tank 10 in pure procedure, which in the course of work done holding 10 side of liquid tank
Vertical up-or-down movement.
Second embodiment
A kind of separating and extracting process is being present embodiments provided, this method comprises: providing separation according to first embodiment
Extraction element；Fixator load punctures component, punctures component and punctures kit one by one；It is default that fixator loads liquid transfer gun head transfer
Reagent, which enters, holds liquid tank, and loads magnet and carry out magnetic to biomolecule, and shift waste liquid and enter waste liquid cabin；Wherein, reagent packet is preset
Include lytic reagent, washing reagent and elution reagent；The specific structure of the separating extraction device 1 includes complete in first embodiment
The optional technical solution in portion, at least all beneficial effects brought by the technical solution with first embodiment.
Fig. 1 to Fig. 6 is please referred to, experimenter is as follows using the course of work of the separating and extracting process:
Step 1: taking off film manually, i.e., the first protective film 17 and first that liquid tank 10 is held in kit 120 is first placed into cabin 40
It is opened manually with the third protective film 43 at the top in the second placement cabin 50.
Step 2: being put into kit, i.e., liquid tank 10 is held into the addition of sample liquid manually, and kit 120 is packed into as required
Fixed frame 90.
Step 3: puncturing, first placement cabin 40 moves to the following position directly of fixator 83 in software control kit 120,
The control Single Mechanical arm 81 of third dynamical system 125 drives fixator 83 to move downward and fasten and punctures component, then third power
System 125 controls Single Mechanical arm 81 and fixator 83 is driven to move upwards, when leaving kit 120 wait puncture component bottom end, by list
Location sensitive detection system provides signal in mechanical arm 81, and the second dynamical system 123 is run simultaneously with the first dynamical system 121,
Control kit 120 moves on the second sliding rail 123b and the first sliding rail 121b, then will store reagent in reagent storage cabin 30
Reagent storage chamber and waste liquid cabin 20 move to the underface for installing the fixator 83 for puncturing component additional, 12 determined by positioning mark
Then position controls the fixator 83 that Single Mechanical arm 81 drives installation to puncture component by third dynamical system 125 and moves up and down one by one
It punctures；After the reagent storage chamber of storage reagent and the positioning mark 12 in waste liquid cabin 20 all puncture in candidate agent storage cabin 30, by soft
Second placement cabin 50, which moves to install additional, in part control kit 120 punctures immediately below the fixator 83 of component, third dynamical system 125
The fixator 83 that the control drive installation of Single Mechanical arm 81 punctures component, which moves downwardly to the second placement cabin 50 and unloads, punctures component.
Step 4: cracking, cracking specific steps are as follows:
1. load liquid transfer gun head: third dynamical system 125 controls Single Mechanical arm 81 and fixator 83 is driven to move up, to solid
When determining 83 bottom end of device and leaving kit 120, signal, the second dynamical system are provided by location sensitive detection system in Single Mechanical arm 81
123 control kits 120 are moved in the second sliding rail 123b, until the first placement cabin 40 moves to the underface of fixator 83, third is dynamic
Force system 125 controls Single Mechanical arm 81 and fixator 83 is driven to move down and load liquid transfer gun head 41；
2. adding lytic reagent: third dynamical system 125 controls the fixator that Single Mechanical arm 81 drives load liquid transfer gun head 41
83 move up, and when kit 120 is left in 41 bottom end of liquid transfer gun head, are provided by location sensitive detection system in Single Mechanical arm 81
Signal, the second dynamical system 123 are run simultaneously with the first dynamical system 121, and control kit 120 is in the second sliding rail 123b and the
It is moved on one sliding rail 121b, the separate storage chamber for filling lytic reagent in reagent storage cabin 30 is then moved into loaded holders 83
The underface of liquid transfer gun head 41, third dynamical system 125 control the liquid transfer gun head 41 that Single Mechanical arm 81 drives loaded holders 83
It moves downward and passes through the pump housing 130 and be automatically drawn into quantitative cleavage reagent, then third dynamical system 125 controls 81 band of Single Mechanical arm
The fixator 83 of dynamic load liquid transfer gun head 41 moves upwards, when kit 120 is left in 41 bottom end of liquid transfer gun head, the second dynamical system
System 123 is run simultaneously with the first dynamical system 121, and control kit 120 is transported on the second sliding rail 123b and the first sliding rail 121b
It is dynamic, then the positioning mark 12 that liquid tank 10 is held in kit 120 is moved to the underface of the fixator 83 of load liquid transfer gun head 41, the
Three dynamical systems 125 control Single Mechanical arm 81 drives the fixator 83 of load liquid transfer gun head 41 to move downward insertion and holds liquid tank 10
Lytic reagent addition is held liquid tank 10 automatically by the pump housing 130 by positioning mark 12；
3. unloading liquid transfer gun head: third dynamical system 125 controls the fixation that Single Mechanical arm 81 drives load liquid transfer gun head 41
Device 83 moves upwards, when kit 120 is left in 41 bottom end of liquid transfer gun head, the second dynamical system 123 and the first dynamical system 121
It runs simultaneously, control kit 120 moves in the second dynamical system 123 and the second sliding rail 123b, then places cabin for first
40 move to the underface of the fixator 83 of load liquid transfer gun head 41, and third dynamical system 125 controls Single Mechanical arm 81 and drives load
The fixator 83 of liquid transfer gun head 41 moves downward and unloads liquid transfer gun head 41 automatically；
4. magnetic: third dynamical system 125 controls Single Mechanical arm 81 and fixator 83 is driven to move upwards, 83 bottom of device to be fixed
When leaving kit 120, the second dynamical system 123 is run simultaneously with the first dynamical system 121 at end, controls kit 120 the
It is moved on two dynamical systems 123 and the second sliding rail 123b, the magnet spare bits 103 for placing magnet 100 is then moved into fixator
83 underface, third dynamical system 125 control Single Mechanical arm 81 and fixator 83 are driven to move downward and load magnet automatically
100, third dynamical system 125 controls Single Mechanical arm 81 and the fixator 83 of load magnet 100 is driven to move upwards, to magnet 100
Bottom end when leaving fixed frame 90, the second dynamical system 123 is run simultaneously with the first dynamical system 121, and control kit 120 exists
It is moved on second dynamical system 123 and the second sliding rail 123b, then magnet magnetic position 101 is moved to the fixation of load magnet 100
The underface of device 83, after the completion of cracking reaction, third dynamical system 125 controls Single Mechanical arm 81 and drives load magnet 100
Fixator 83 moves downward, and moves vertically holding 10 side of liquid tank, to magnetic at the end of, by magnet 100 in magnet magnetic position
101 unloadings；
5. moving waste liquid: third dynamical system 125 controls Single Mechanical arm 81 and fixator 83 is driven to move upwards, device 83 to be fixed
When fixed frame 90 is left in bottom end, the second dynamical system 123 is run simultaneously with the first dynamical system 121, and control kit 120 is the
It is moved on two sliding rail 123b and the first sliding rail 121b, the first placement cabin 40 is being moved into the fixator 83 of load liquid transfer gun head 41 just
Lower section, third dynamical system 125 control Single Mechanical arm 81 and drive fixator 83 to move downward and load liquid transfer gun head 41 automatically, the
Three dynamical systems 125 control Single Mechanical arm 81 drives fixator 83 to move upwards, and leaves kit to 41 bottom end of liquid transfer gun head
120, the second dynamical system 123 is run simultaneously with the first dynamical system 121, and control kit 120 is in the second sliding rail 123b and the
It is moved on one sliding rail 121b, then will hold positioning mark 12 in liquid tank 10 and move to 83 underface of fixator for installing liquid transfer gun head 41 additional,
Third dynamical system 125 controls Single Mechanical arm 81 and drives the fixator 83 for installing liquid transfer gun head 41 additional to move downward and be inserted into positioning mark
12, the fixator 83 of waste liquid sucking installation liquid transfer gun head 41 in liquid tank 10 will be held by the pump housing 130, third dynamical system 125 is controlled
Single Mechanical arm 81 processed drives the fixator 83 for installing liquid transfer gun head 41 additional to move upwards, the second dynamical system 123 and the first dynamical system
System 121 is run simultaneously, and control kit 120 moves on the second sliding rail 123b and the first sliding rail 121b, then in waste liquid cabin 20
Positioning mark 12 moves to the underface for installing the fixator 83 of liquid transfer gun head 41 additional, and third dynamical system 125 controls 81 band of Single Mechanical arm
The dynamic fixator 83 for installing liquid transfer gun head 41 additional is moved downward and is inserted into the positioning mark 12 in waste liquid cabin 20, will be given up by the pump housing 130
Liquid is discharged into waste liquid cabin 20；
6. magnet is moved to spare bits: third dynamical system 125 controls Single Mechanical arm 81 and drives installation liquid transfer gun head 41
Fixator 83 moves upwards, when kit 120 is left in 41 bottom end of liquid transfer gun head, the second dynamical system 123 and the first dynamical system
System 121 is run simultaneously, and control kit 120 moves on the second sliding rail 123b and the first sliding rail 121b, places cabin 40 for first
The underface of the fixator 83 of load liquid transfer gun head 41 is moved to, third dynamical system 125 controls Single Mechanical arm 81 and load is driven to move
The fixator 83 of liquid pipette tips 41 moves downward and unloads liquid transfer gun head 41 automatically, and third dynamical system 125 controls Single Mechanical arm 81
Fixator 83 is driven to move upwards, when kit 120 is left in 83 bottom end of device to be fixed, the second dynamical system 123 and the first power
System 121 is run simultaneously, and control kit 120 moves on the second sliding rail 123b and the first sliding rail 121b, then will place magnetic
The magnet magnetic position 101 of body 100 moves to the underface of fixator 83, and third dynamical system 125 controls Single Mechanical arm 81 and drives admittedly
Determine device 83 and move downward and load automatically magnet 100, third dynamical system 125 controls Single Mechanical arm 81 and drives load magnet 100
Fixator 83 move upwards, when fixed frame 90 is left in 100 bottom end of magnet, the second dynamical system 123 and the first dynamical system
121 run simultaneously, and control kit 120 moves on the second sliding rail 123b and the first sliding rail 121b, and magnet spare bits 103 are moved
To the fixator 83 of load magnet 100, third dynamical system 125 controls Single Mechanical arm 81 and drives load magnet 100
Fixator 83 moves downward and unloads magnet 100 automatically.
Step 5: washing, washing step is similar with cleavage step, also divides 6 steps；It, can depending on washing times are because of sample type
Be 1 time or repeatedly；
1. loading liquid transfer gun head: with the 1 of the 4th step；
2. adding washing reagent: with the 2 of the 4th step, only changing lytic reagent into washing reagent；
3. unloading liquid transfer gun head: with the 3 of the 4th step；
4. magnetic: with the 4 of the 4th step；
5. moving waste liquid: with the 5 of the 4th step；
6. magnet is moved to spare bits: with the 6 of the 4th step；
Step 6: elution, 4 steps of elution step point；
1. loading liquid transfer gun head: with the 1 of the 4th step；
2. adding elution reagent: with the 2 of the 4th step, only changing lytic reagent into elution reagent；
3. unloading liquid transfer gun head: with the 3 of the 4th step；
4. magnetic: with the 4 of the 4th step, to the end of reacting, fixator 83 is placed into cabin 40 first and installs liquid transfer gun head 41 additional,
Final purified product in liquid tank 10 will be held to shift by setting, complete operation.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of separating extraction device, which is characterized in that including pedestal, and support construction on the base is set,
Moving assembly is provided in the support construction, the moving assembly includes fixator, and the fixator includes adapter, described
It adapter and liquid transfer gun head and punctures component and is detachably connected, the fixator passes through in the adapter and the liquid transfer gun head
The channel of setting is connected to, and the fixator is connect with the pump housing by conduit, sucks the liquid transfer gun head or liquid is discharged, in institute
It states and is additionally provided with sliding rail on pedestal, the sliding rail is slidably connected with fixed frame, and kit is provided in the fixed frame, described
Kit includes holding liquid tank and waste liquid cabin, described to hold liquid tank and the useless tank roof is equipped with opening, is set over said opening
There is Ka Gai, be provided with elaxtic seal between the opening and card lid, be additionally provided with magnet on the fixed frame,
The magnet can be detachably connected with the adapter.
2. separating extraction device according to claim 1, which is characterized in that the moving assembly further includes Single Mechanical arm,
The Single Mechanical arm is fixedly connected with the fixator.
3. separating extraction device according to claim 1, which is characterized in that further include XYZ dynamical system, the XYZ is dynamic
Force system is used to control the fixed frame respectively in X-direction, the movement of Y direction and the control moving assembly in Z axis
Direction is mobile.
4. separating extraction device according to claim 1, which is characterized in that the kit further include reagent storage cabin,
First places cabin and second places cabin, and the card, which covers to be provided with, holds liquid tank and the waste liquid cabin is two corresponding with described respectively
Through-hole, described hold are provided with dismountable first protective film on liquid tank；
The reagent storage cabin includes multiple reagent storage chambers, and the opening of the waste liquid cabin and the reagent storage chamber is installed with
Second protective film；
The first placement cabin and second placement cabin place respectively the liquid transfer gun head and it is described puncture component, described first
It places and is equipped with dismountable third protective film at the top of cabin and second placement cabin.
5. separating extraction device according to claim 1, which is characterized in that the elaxtic seal includes silica gel, rubber
At least one of glue.
6. separating extraction device according to claim 4, which is characterized in that be provided on the elaxtic seal and institute
State the corresponding positioning mark of through-hole.
7. separating extraction device according to claim 2, which is characterized in that the two sides in the reagent storage cabin are respectively set
There are two fixed ears.
8. separating extraction device according to claim 4, which is characterized in that the liquid transfer gun head is disposable liquid-transfering gun
Head, the component that punctures is disposable aspiration needle.
9. separating extraction device according to claim 1, which is characterized in that the magnet is magnet or electromagnet.
10. a kind of separating and extracting process, which is characterized in that the described method includes:
Separating extraction device according to claim 1 is provided；
Component is punctured described in the fixator load, the component that punctures punctures the kit one by one；
The fixator load default reagent of liquid transfer gun head transfer holds liquid tank described in entering, and loads the magnet and divide biology
Son carries out magnetic, and shifts waste liquid and enter the waste liquid cabin；
Wherein, default reagent includes lytic reagent, washing reagent and elution reagent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811110374.3A CN108977344A (en) | 2018-09-21 | 2018-09-21 | A kind of separating extraction device and separating and extracting process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811110374.3A CN108977344A (en) | 2018-09-21 | 2018-09-21 | A kind of separating extraction device and separating and extracting process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108977344A true CN108977344A (en) | 2018-12-11 |
Family
ID=64546541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811110374.3A Pending CN108977344A (en) | 2018-09-21 | 2018-09-21 | A kind of separating extraction device and separating and extracting process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108977344A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110286110A (en) * | 2019-07-29 | 2019-09-27 | 邓鑫 | A kind of hepatocarcinoma early diagnosis kit |
| WO2021031227A1 (en) * | 2019-08-22 | 2021-02-25 | 南京中大生物信息研究院有限公司 | Integrated nucleic acid detection device and application |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120115238A1 (en) * | 2009-07-09 | 2012-05-10 | Toppan Printing Co., Ltd. | Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus |
| CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| CN103789198A (en) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acids |
| CN106367309A (en) * | 2016-11-22 | 2017-02-01 | 安图实验仪器(郑州)有限公司 | Single-row nucleic acid extraction box applicable to nucleic acid diagnostic systems |
| CN206930487U (en) * | 2017-07-25 | 2018-01-26 | 上海昂鹭生物技术有限公司 | A kind of fully automatic liquid processing system |
| WO2018049926A1 (en) * | 2016-09-19 | 2018-03-22 | 清华大学 | Automatic liquid handling system |
| CN108004324A (en) * | 2017-12-29 | 2018-05-08 | 东南大学 | One kind is used for tumour cell nucleic acid aptamers screening circular card box device |
| CN209322849U (en) * | 2018-09-21 | 2019-08-30 | 西安佰奥莱博生物科技有限公司 | A kind of separating extraction device and separation and Extraction system |
-
2018
- 2018-09-21 CN CN201811110374.3A patent/CN108977344A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120115238A1 (en) * | 2009-07-09 | 2012-05-10 | Toppan Printing Co., Ltd. | Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus |
| CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| CN103789198A (en) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acids |
| WO2018049926A1 (en) * | 2016-09-19 | 2018-03-22 | 清华大学 | Automatic liquid handling system |
| CN106367309A (en) * | 2016-11-22 | 2017-02-01 | 安图实验仪器(郑州)有限公司 | Single-row nucleic acid extraction box applicable to nucleic acid diagnostic systems |
| CN206930487U (en) * | 2017-07-25 | 2018-01-26 | 上海昂鹭生物技术有限公司 | A kind of fully automatic liquid processing system |
| CN108004324A (en) * | 2017-12-29 | 2018-05-08 | 东南大学 | One kind is used for tumour cell nucleic acid aptamers screening circular card box device |
| CN209322849U (en) * | 2018-09-21 | 2019-08-30 | 西安佰奥莱博生物科技有限公司 | A kind of separating extraction device and separation and Extraction system |
Non-Patent Citations (1)
| Title |
|---|
| 王晓民 上海：上海交通大学出版社: "临床生物样本库的探索与实践", pages: 53 - 54 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110286110A (en) * | 2019-07-29 | 2019-09-27 | 邓鑫 | A kind of hepatocarcinoma early diagnosis kit |
| CN110286110B (en) * | 2019-07-29 | 2024-03-19 | 邓鑫 | Early diagnosis kit for liver cancer |
| WO2021031227A1 (en) * | 2019-08-22 | 2021-02-25 | 南京中大生物信息研究院有限公司 | Integrated nucleic acid detection device and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9073052B2 (en) | Lab members and liquid handling systems and methods including same | |
| JP4230816B2 (en) | Plate that automatically stores part of the sample | |
| CN209322849U (en) | A kind of separating extraction device and separation and Extraction system | |
| EP2058664B1 (en) | Apparatus and method for handling fluids for analysis | |
| CN105334333B (en) | A kind of whole blood measuring instrument and automatic sampling apparatus being easily changed latex reagent | |
| TWI816777B (en) | System for extracting biomolecules from a sample and related methods | |
| CN108977344A (en) | A kind of separating extraction device and separating and extracting process | |
| BRPI0717331B1 (en) | DEVICE FOR AUTOMATIC SETTING OF THE BACTERIAL INOCULUS LEVEL OF A SAMPLE | |
| CN111693354A (en) | Frozen slice immunohistochemical staining machine and method | |
| JP7057813B2 (en) | Pipetting unit and pipetting method for closed liquid containers | |
| EP1615037A1 (en) | An apparatus for liquid handling with multiple transfer tools | |
| CN115248328A (en) | POCT blood cell analyzer and using method thereof | |
| CN111855995A (en) | Incubation device | |
| CN212845408U (en) | Incubation device | |
| US20220395784A1 (en) | Mixing liquids using an automated liquid handling system | |
| CN114323783B (en) | Sampling method, sampling assembly and sample analyzer | |
| EP4344776A1 (en) | Test cartridges and systems | |
| CN218202789U (en) | Circulating tumor cell extraction system | |
| CN216525867U (en) | POCT blood cell analyzer | |
| CN216236988U (en) | High-throughput automatic protein transfection platform | |
| CN214066682U (en) | Frozen section immunohistochemical dyeing machine | |
| CN115232735A (en) | Flow type sample preparation instrument | |
| WO2024102979A2 (en) | Consumable for centrifugation of biological cells | |
| CN115248321A (en) | POCT blood cell analyzer and using method thereof | |
| CN115248322A (en) | POCT blood cell analyzer and using method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |