CN108948220A - A kind of low viscosity, the preparation method of the prebiotic active polysaccharide of highly dissoluble longan - Google Patents
A kind of low viscosity, the preparation method of the prebiotic active polysaccharide of highly dissoluble longan Download PDFInfo
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Abstract
A kind of low viscosity, the preparation method of the prebiotic active polysaccharide of highly dissoluble longan, comprising the following steps: S1: pretreatment: taking longan pulp, is dried for standby after it is pre-processed with ethyl alcohol immersion.S2: it pulverizes: by longan pulp cryogenic ultramicro pulverization described in the step S1, obtaining longan pulp Ultramicro-powder；S3: by longan pulp Ultramicro-powder described in the step S2, water is added according to solid-liquid ratio, heating extraction collects extracting solution, is concentrated and dried to obtain extract；S4: it is extracted extract obtained in the step S3, obtains extract liquor；S5: gained extract liquor in the step S4 is dialysed, dry longan polysaccharide.Longan polysaccharide preparation method of the present invention is simple, at low cost, is easy to large-scale production.There is the characteristic that viscosity is small, dissolubility is good by pulverizing the longan polysaccharide extracted, the longan polysaccharide of preparation has better prebiotic activity than the longan polysaccharide of traditional extraction, can be used for the exploitation of longan functional food, there is important meaning to the intensive processing of longan.
Description
Technical field
The present invention relates to functional food preparation field, in particular to the prebiotic activity of a kind of low viscosity, highly dissoluble longan is more
The preparation method of sugar.
Background technique
Longan is typical dual-purpose of drug and food food, be used to treat in Traditional Chinese Medicine or improvement insufficiency of vital energy and blood, palpitaition are seized with terror
Sad, insomnia forgetfulness, anaemia, splenasthenic diarrhea etc..Modern studies have found that its main function ingredient is polysaccharide, longan pulp polysaccharide tool
There are the multiple efficacies such as immunological regulation, enhancing memory, anti-oxidant.Thus, the correlative study of longan pulp polysaccharide causes extensive pass
Note.Polyoses content may be up to 35% in longan pulp, can significantly adjust Intestinal Mucosal Immunization system, enhance immunosupress and just
The function of immune system of normal mouse, and Mesenteric lymph node cell, splenic lymphocytes and macrophage can be directly stimulated in vitro
Deng activation；Also it can improve learning and memory function by adjusting cholinergic nerve system.Longan pulp polysaccharide is as natural activity function
The energy factor has wide exploitation prospect.But the longan polysaccharide that the prior art extracts preparation has that dissolubility is poor, viscosity
The disadvantages of big, limits the development and utilization to longan polysaccharide, and there is an urgent need to the technologies for developing new to solve the problems, such as this.
The longan polysaccharide of conventional hot water's extraction method preparation is because molecular weight is big, and strand is mutually handed over because of hydrogen bond action when being dissolved in water
Connection, causes its viscosity big, is easy to aggregate and precipitate in the solution, dissolubility is small.The present invention is established by using the method for ultramicro grinding
A kind of preparation method for reducing polysaccharide viscosity, improving deliquescent longan polysaccharide.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of low viscosities, the prebiotic work of highly dissoluble longan
The preparation method of property polysaccharide.
In order to solve the above technical problem, the present invention provides a kind of low viscosities, highly dissoluble longan prebiotic active polysaccharide
Preparation method, comprising the following steps:
S1: pretreatment: taking longan pulp, is dried for standby after it is pre-processed with ethyl alcohol immersion.
S2: it pulverizes: longan pulp described in the step S1 being pulverized, longan pulp Ultramicro-powder is obtained；
S3: it extracts: by longan pulp Ultramicro-powder described in the step S2, water is added according to solid-liquid ratio, heating extraction is received
Collect extracting solution, is concentrated and dried to obtain extract；
S4: it extraction: is extracted extract obtained in the step S3, obtains extract liquor；
S5: dialysis separation: gained extract liquor in the step S4 is dialysed, dry longan polysaccharide.
Due in addition to longan polysaccharide, also having the impurity of many alcohol-solubles, such as pigment, oligosaccharide in longan pulp, because
This, in the step S1, the pretreatment impregnated using ethyl alcohol is mainly used for removing impurity, reduces the viscosity of longan pulp,
Recovery rate is improved simultaneously.It since the longan pulp humidity after alcohol steep is larger, can not be pulverized, therefore, it is necessary to will
Longan pulp is dried for standby.
Superfine communication technique is using the methods of supersonic jet mill, the crushing of cold slurry, with previous purely mechanic crushing side
Method is entirely different.Hot-spot phenomenon will not be generated in crushing process, or even can be crushed under low-temperature condition, speed
Fastly, moment can be completed, thus retain the bioactive ingredients of powder to the maximum extent, in favor of required high-quality volume production is made
Product.Due to using supersonic jet mill, the distribution of power is quite uniform on raw material.The setting of hierarchy system, it is both stringent to limit
Bulky grain has been made, had avoided the occurrence of broken, has obtained the uniform superfine powder of particle diameter distribution, while largely increasing micro mist
Specific surface area increases accordingly adsorptivity, dissolubility etc. also.Object is after pulverizing, the superfine powder one of nearly nanometer fine grain
As can be directly used for preparation production, and the product routinely crushed can be only achieved there is still a need for some intermediate links and be directly used in production
Requirement be likely to cause wastage of material in this way.Ultramicro grinding is carried out under closed system, has both been avoided around micro mist pollution
Environment, and the dust polluted product in air can be prevented.
In the step S2, the longan pulp dried in step S1 is pulverized, using oscillatory type cell
Longan pulp is carried out ultramicro crushing treatment by grade micronizer, on the one hand raw material can be crushed to 1nm~100 μm, greatly
Reduce partial size, increases surface area so that raw material be made to be easy to contact with extractant and promote its dissolution, obtain higher extraction yield；
On the other hand, the mechanical shear stress of ultramicro grinding can be such that polysaccharide main chain and/or branched structure is broken, and destroy the hydrogen of molecule interchain
Key increases its dissolubility to reduce its viscosity in the solution.
By longan pulp Ultramicro-powder described in the step S2, water is added according to solid-liquid ratio, heating extraction is collected and extracted
Liquid is concentrated and dried to obtain extract.Since longan pulp have passed through pretreatment, a large amount of impurity is had been removed, longan is improved
The content of longan polysaccharide in pulp, and have passed through ultramicro grinding, the surface area of longan pulp is increased, when so that hot water extracting,
Longan polysaccharide can be promoted to be dissolved in solvent, the mechanical shear stress of ultramicro grinding can be such that longan polysaccharide main chain and/or branch chains
Structure fracture, the hydrogen bond for destroying molecule interchain increase its dissolubility, further improve to reduce its viscosity in the solution
The recovery rate of longan polysaccharide, while reducing the viscosity of longan polysaccharide in finished product.After the completion of extraction, by being concentrated in vacuo drying,
Obtain longan polysaccharide crude extract.
It, can be thick to longan polysaccharide by the way of extraction due to containing lipophilic contaminant in longan polysaccharide crude extract
Extract is further purified, and after extraction is completed, obtains extract.Dialysis in the solution may be used using small-molecule substance
By semi-permeable membrane, and macromolecular substances cannot reach the method for separation by the property of semi-permeable membrane.Since polysaccharide component is general
Molecular weight is larger, and in order to remove the impurity of small molecule, therefore the present invention selects bag filter by polysaccharide component and small molecule chemical combination
Object is separated, and the longan polysaccharide of highly dissoluble and low viscosity is further obtained.
Therefore, the present invention removes impurity by alcohol pre-treatment, reduces longan polysaccharide viscosity using superfine communication technique, most
The longan polysaccharide of low viscosity, high-dissolvability is obtained eventually, and this method step is simple, and required instrument is few, and production cost is low, can use
In industrialization promotion.
Further, in the step S1, the concentration of the ethyl alcohol is 80%~95%.
Since the purpose of step S1 mainly cleans, particular for impurity such as pigment and oligosaccharide, and select 80%~
95% ethyl alcohol cleans, and can remove most pigment and oligosaccharide etc. with fat-soluble impurity, without to more
Sugared content impacts.
Further, in the step S1, the ethyl alcohol soaking time is 20~30h, 35~50 DEG C of 24~48h of drying.
The time that ethyl alcohol impregnates, the too short impurity that may cause leached not exclusively, when influencing the purity of longan polysaccharide, and leaching
Between reach saturation after, continue leach will cause the production time increase, therefore preferred alcohol soaking time be 20~30h.
The temperature wherein dried also has certain requirement, and since polysaccharide viscosity itself is also bigger, temperature is excessively high to be made
At the destruction of ingredient, subsequent ultramicro grinding is also influenced, and temperature is too low, and drying degree can be made not reach requirement, or makes
It is too long to obtain drying time, equally influence production efficiency, therefore selecting drying temperature is 35~50 DEG C, and the setting of drying time is same
Sample needs to consider the problems such as production cost and target component stability, therefore, comprehensively considers, selects drying time for 24~48h
It is advisable, has not only ensure that drying sufficiently, but also will not damage to effective component.
Further, in the step S2, the time of ultramicro crushing treatment is 1~5min.
The cryogenic ultramicro pulverization time that the present invention selects be 1~5min because in a certain range with pulverize when
Between extension, the influence to material particular diameter and polysaccharide is bigger, but after being above a certain range, pulverizes the time and influences on it
Will not change as the time increases, from treatment effect and it is energy saving from the point of view of, it is currently preferred using super
Crushing of Ultrafine handles 1~5min.
Further, in the step S2, described pulverize is cryogenic ultramicro pulverization, and the temperature is -17~-20
℃。
It is converted into thermal energy due in mechanicalness crushing process, having part kinetic energy, it is therefore, higher in order to retain target product
Activity, present invention selection pulverizes under -17~-20 DEG C of environment, to guarantee that it is preferably raw that the product obtained has
Object activity.
Further, in the step S3, according to the mass volume ratio of longan pulp Ultramicro-powder and distilled water be 1:30~
50, extraction conditions are 80~90 DEG C of 3~5h of extraction.
Since the solubility of target component all has certain limit, wherein the ratio of longan pulp and distilled water is too high,
The dissolution of longan polysaccharide reaches saturation state, can make the part longan polysaccharide in longan pulp that can not continue to dissolve, cause dragon
A large amount of longan polysaccharides are remained in eye pulp, cause the waste of raw material, increases production and rises this；And the ratio of longan pulp and distilled water
It is too low, recovery rate can be made too low, and increase and extract duration, equally increase production cost, therefore the present invention selects longan pulp
It is extracted with distilled water according to 1:30~50, to reach optimum extraction balance.
Since polysaccharide can make polysaccharide component be dissolved in water to greatest extent for hydroaropic substance by heating extraction
In.Wherein Extracting temperature and extraction time are also the big factors for determining recovery rate, and temperature is too high or extraction time too long possibility
Polysaccharide chain break is caused, the destruction of target component is caused, and causes the waste of the energy, and temperature is too low or extraction time is too short
Meeting so that extraction is insufficient, influences extraction efficiency so that recovery rate is too low, and therefore, selective extraction condition is 80~90 DEG C and extracts 3
~5h, it is preferred that extraction conditions are 85 DEG C of extraction 4h.
Preferably, in the step S3, extraction conditions are 85 DEG C of extraction 4h.
Further, in the step S4, extraction conditions is the building of diisooctyl succinate sodium sulfonate (AOT)-isooctane
Reverse micelle system.
AOT (diisooctyl succinate sodium sulfonate) can form stable reverse micelle system, be most common surfactant.
Inverse micelle abstraction technique, by the way that surfactant molecule to be added in organic solvent, is dispersed based on surfactant
Form " pond " structure that nanoscale hydrophobic group is outside, hydrophilic group is inside, the large biological molecules such as polysaccharide using polarity act on into
Enter " pond " structure, reverse micelle " pond " utilizes the phase interaction between hydrophobic force, ionization power, electrostatic force and hydroxyl
Firmly etc., promote polar substances " solubilising " to enter, to realize the extraction to extract.It includes preceding extraction and rear extraction two
Step, preceding extraction is that extract and inverse micellar solution act on, into " pond " of reverse micelle；Extraction is the extraction in " pond " afterwards
Object is transferred to water phase from reverse micelle, to realize the effect of separation.Inverse micelle abstraction is mainly used in protein, amino at present
The extraction of the substances such as acid, antibiotic, nucleic acid, polysaccharide.Compared to traditional separating and purifying technology, reverse micelle system have efficiently, at
This is low, is easy to amplify, and the characteristics of can be recycled.
Further, the reverse micelle system mainly by AOT- isooctane (v:v=3:1) solution of 30~50mmol/L and
It is formed in equal volume containing 50~100mmol/L NaCl and 5~15mmol/L guanidine hydrochloride solution mixed solution.
Further, the extracting process is that longan pulp polyoses extract is added according to the ratio of 0.5~2 (mg/mL)
After reverse micelle system, after 200~300rpm vibrates 0.5~2h, stratification collects reverse micelle phase；Again by the reverse micelle of collection
Mutually mixed with isometric aqueous solution containing 100~200mmol/L NaCl and 0.1~0.2mol/L guanidine hydrochloride, 200~
After 300rpm vibrates 0.5~2h, stratification collects water phase, obtains extract liquor.
Further, in the step S5, condition of dialysing are as follows: use the bag filter that molecular weight is 3000~8000Da that shuts off
It dialyses 2~4 days at 4 DEG C.
Since polysaccharide component can not penetrate in 3000~8000Da bag filter, select bag filter can be further
Longan polysaccharide is purified, other impurities ingredient will not be introduced, more securely and reliably.
Further, the present invention provides a kind of longan polysaccharide, is made according to above-mentioned extracting method.
Beneficial effects of the present invention:
(1) longan polysaccharide preparation method of the present invention is simple, at low cost, is easy to large-scale production.
(2) present invention has the characteristic that viscosity is small, dissolubility is good by pulverizing the longan polysaccharide extracted.
(3) longan polysaccharide prepared by the present invention has better prebiotic activity than the longan polysaccharide of traditional extraction, can be used for
The exploitation of longan functional food has important meaning to the intensive processing of longan.
Detailed description of the invention
Fig. 1 is that conventional hot water extracts longan polysaccharide and ultramicro grinding extracts what longan polysaccharide was proliferated lactobacillus bulgaricus
It influences.
Specific embodiment
To keep the purposes, technical schemes and advantages of the invention implemented clearer, below in conjunction in the embodiment of the present invention
Attached drawing, technical solution in the embodiment of the present invention is further described in more detail.In the accompanying drawings, identical from beginning to end or class
As label indicate same or similar element or element with the same or similar functions.Described embodiment is the present invention
A part of the embodiment, instead of all the embodiments.The embodiments described below with reference to the accompanying drawings are exemplary, it is intended to use
It is of the invention in explaining, and be not considered as limiting the invention.Based on the embodiments of the present invention, ordinary skill people
Member's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.Under
Face is described in detail the embodiment of the present invention in conjunction with attached drawing.
Embodiment 1
The present embodiment provides a kind of low viscosities, the preparation method of the prebiotic active polysaccharide of highly dissoluble longan, including following step
It is rapid:
Pretreatment: taking 100g longan pulp, and after the immersion for 24 hours of 80% ethyl alcohol is added, 40 DEG C of drying are for 24 hours.
Pulverize: at a temperature of -17 DEG C of use, oscillatory type cell grade micronizer pulverizes longan pulp
2min。
It extracts: distilled water is added according to mass volume ratio (g/mL) 1:30, after 85 DEG C of extractions 4h, cross and filter out residue, collection
It is concentrated in vacuo after leaching liquor, is freeze-dried to obtain longan extract.
Extraction: by the AOT- isooctane (v:v=3:1) of 30mmol/L and it is isometric containing 50mmol/L NaCl with
The aqueous solution mixing of 10mmol/L guanidine hydrochloride prepares inverse micellar solution.Extract is added instead according to the ratio of 0.5:1 (mg/mL)
After micellar solution, is stood after 250rpm oscillation 1h, collect lower layer's inverse micellar solution；It is contained into 100mmol/ with isometric again
The aqueous solution of L NaCl and 0.15mol/L guanidine hydrochloride mixes, and stands after 250rpm oscillation 1h, collects upper layer aqueous solution.
Dialysis: use the bag filter that molecular weight is 8000Da that shuts off to dialyse and dialyse at 4 DEG C 3d, be freeze-dried and obtain longan
Polysaccharide.
Embodiment 2
Pretreatment: taking 100g longan pulp, after the immersion for 24 hours of 80% ethyl alcohol is added, 40 DEG C of drying 48h.
It pulverizes: longan pulp being pulverized by 3min using low temperature shock formula cell grade micronizer, temperature is set
It is set to -20 DEG C.
It extracts: distilled water is added according to mass volume ratio (g/mL) 1:40, after 85 DEG C of extractions 4h, cross and filter out residue, collection
It is concentrated in vacuo after leaching liquor, is freeze-dried to obtain longan extract.
Extraction: by the AOT- isooctane (v:v=3:1) of 40mmol/L and it is isometric containing 80mmol/L NaCl with
The aqueous solution mixing of 15mmol/L guanidine hydrochloride prepares inverse micellar solution.Anti- glue is added according to the ratio of 1:1 (mg/mL) in extract
After beam solution, is stood after 200rpm oscillation 1.5h, collect lower layer's inverse micellar solution；It is contained into 160mmol/ with isometric again
The aqueous solution of L NaCl and 0.1mol/L guanidine hydrochloride mixes, and stands after 200rpm oscillation 1.5h, collects upper layer aqueous solution.
Dialysis: use the bag filter that molecular weight is 8000Da that shuts off to dialyse and dialyse at 4 DEG C 3d, be freeze-dried and obtain longan
Polysaccharide.
Embodiment 3
Pretreatment: taking 100g longan pulp, after the immersion for 24 hours of 80% ethyl alcohol is added, 50 DEG C of drying 36h.
It pulverizes: longan pulp is pulverized by 5min, medium temperature using low temperature shock formula cell grade micronizer
Degree is set as -18 DEG C.
It extracts: distilled water is added according to mass volume ratio (g/mL) 1:50, after 85 DEG C of extractions 4h, cross and filter out residue, collection
It is concentrated in vacuo after leaching liquor, is freeze-dried to obtain longan extract.
Extraction: by the AOT- isooctane (v:v=3:1) of 50mmol/L and it is isometric containing 100mmol/L NaCl with
The aqueous solution mixing of 5mmol/L guanidine hydrochloride prepares inverse micellar solution.Anti- glue is added according to the ratio of 2:1 (mg/mL) in extract
After beam solution, is stood after 300rpm oscillation 0.5h, collect lower layer's inverse micellar solution；It is contained into 200mmol/ with isometric again
The aqueous solution of L NaCl and 0.2mol/L guanidine hydrochloride mixes, and stands after 300rpm oscillation 0.5h, collects upper layer aqueous solution.
Dialysis: use the bag filter that molecular weight is 8000Da that shuts off to dialyse and dialyse at 4 DEG C 3d, be freeze-dried and obtain longan
Polysaccharide.
Embodiment 4
Pretreatment: taking 100g longan pulp, after 95% ethyl alcohol immersion 20h is added, 35 DEG C of drying 30h.
It pulverizes: longan pulp is pulverized by 5min, medium temperature using low temperature shock formula cell grade micronizer
Degree is set as -20 DEG C.
It extracts: distilled water is added according to mass volume ratio (g/mL) 1:50, after 80 DEG C of extractions 5h, cross and filter out residue, collection
It is concentrated in vacuo after leaching liquor, is freeze-dried to obtain longan extract.
Extraction: by the AOT- isooctane (v:v=3:1) of 50mmol/L and it is isometric containing 100mmol/L NaCl with
The aqueous solution mixing of 5mmol/L guanidine hydrochloride prepares inverse micellar solution.Anti- glue is added according to the ratio of 2:1 (mg/mL) in extract
After beam solution, is stood after 280rpm oscillation 1.6h, collect lower layer's inverse micellar solution；It is contained into 200mmol/ with isometric again
The aqueous solution of L NaCl and 0.15mol/L guanidine hydrochloride mixes, and stands after 200rpm oscillation 2h, collects upper layer aqueous solution.
Dialysis: use the bag filter that molecular weight is 3000Da that shuts off to dialyse and dialyse at 4 DEG C 4d, be freeze-dried and obtain longan
Polysaccharide.
Embodiment 5
Pretreatment: taking 100g longan pulp, after 90% ethyl alcohol immersion 30h is added, 45 DEG C of drying 28h.
It pulverizes: longan pulp being pulverized by 4min using low temperature shock formula cell grade micronizer, temperature is set
It is set to -19 DEG C.
It extracts: distilled water is added according to mass volume ratio (g/mL) 1:40, after 90 DEG C of extractions 3h, cross and filter out residue, collection
It is concentrated in vacuo after leaching liquor, is freeze-dried to obtain longan extract.
Extraction: by the AOT- isooctane (v:v=3:1) of 50mmol/L and it is isometric containing 50mmol/L NaCl with
The aqueous solution mixing of 12mmol/L guanidine hydrochloride prepares inverse micellar solution.Extract is added instead according to the ratio of 0.5:1 (mg/mL)
After micellar solution, is stood after 300rpm oscillation 0.5h, collect lower layer's inverse micellar solution；It is contained with isometric again
The aqueous solution of 200mmol/L NaCl and 0.18mol/L guanidine hydrochloride mixes, and stands after 300rpm oscillation 0.5h, it is water-soluble to collect upper layer
Liquid.
Dialysis: use the bag filter that molecular weight is 5000Da that shuts off to dialyse and dialyse at 4 DEG C 3d, be freeze-dried and obtain longan
Polysaccharide.
Embodiment 6
The longan polysaccharide that ultramicro grinding method made from 1 method of the present embodiment selection example is extracted compared conventional hot water's leaching
Yield, molecular weight, intrinsic viscosity and the solubility of the longan polysaccharide of formulation preparation, specific determination data are shown in Table 1.Other are implemented
Longan polysaccharide made from example is identical as the technical effect that longan polysaccharide made from embodiment 1 obtains, therefore no longer repeats one by one.
Table 1 pulverizes the nature difference of method extraction and Hot water extraction longan polysaccharide
As it can be seen from table 1 on the one hand the superfine communication technique that the present invention uses can greatly reduce the partial size of raw material, increase
Its surface area promotes its dissolution, obtains higher extraction yield so that it be made to be easy to contact with extractant；On the other hand, ultra micro
The mechanical shear stress of crushing can be such that polysaccharide main chain and/or branched structure is broken, and destroy the hydrogen bond of molecule interchain, thus reduce its
Viscosity in solution increases its dissolubility.
Embodiment 7
The present embodiment to through pulverize extract longan polysaccharide detect its prebiotic effect, using lactobacillus bulgaricus into
One step compares the longan polysaccharide of the longan polysaccharide that extraction is pulverized according to made from 1 method of embodiment and conventional hot water's extraction
To the stimulation of proliferation of probiotics, as shown in Figure 1, having more through pulverizing the longan polysaccharide extracted to lactobacillus bulgaricus
The strong effect of stimulating proliferation shows that it has better prebiotic activity.Longan polysaccharide made from other embodiments can be with this reality
The technical effect that example is agreed is applied, therefore is repeated no more.
Embodiment of above is only used to illustrate the technical scheme of the present invention and not to limit it, although referring to the above preferable embodiment party
Formula describes the invention in detail, those skilled in the art should understand that, it can be to technical solution of the present invention
It modifies or equivalent replacement should not all be detached from the spirit and scope of technical solution of the present invention.Those skilled in the art can also be at this
Other variations etc. are done in spirit and are used in design of the invention, without departing from technical effect of the invention.These
The variation that spirit is done according to the present invention, all should be comprising within scope of the present invention.
Claims (10)
1. the preparation method of a kind of low viscosity, the prebiotic active polysaccharide of highly dissoluble longan, which comprises the following steps:
S1: pretreatment: taking longan pulp, is dried for standby after it is pre-processed with ethyl alcohol immersion；
S2: it pulverizes: longan pulp described in the step S1 being pulverized, longan pulp Ultramicro-powder is obtained；
S3: by longan pulp Ultramicro-powder described in the step S2, being added water according to solid-liquid ratio, and heating extraction collects extracting solution,
It is concentrated and dried to obtain extract；
S4: it is extracted extract obtained in the step S3, obtains extract liquor；
S5: gained extract liquor in the step S4 is dialysed, dry longan polysaccharide.
2. preparation method according to claim 1, which is characterized in that in the step S1, the concentration of the ethyl alcohol is 80%
~95%。
3. preparation method according to claim 1, which is characterized in that in the step S1, the ethyl alcohol soaking time is
20 ~ 30h, 35 ~ 50 DEG C of 24 ~ 48h of drying.
4. preparation method according to claim 1, which is characterized in that in the step S2, the time of ultramicro crushing treatment
For 1 ~ 5min.
5. preparation method according to claim 1, which is characterized in that in the step S2, described pulverize is low temperature
It pulverizes, the temperature is -17 ~ -20 DEG C.
6. preparation method according to claim 1, which is characterized in that in the step S3, according to longan pulp Ultramicro-powder
Mass volume ratio with distilled water is 1:30 ~ 50, and extraction conditions are 80 ~ 90 DEG C of 3 ~ 5h of extraction.
7. described in any item preparation methods according to claim 1, which is characterized in that in the step S3, extraction conditions 85
DEG C extract 4h.
8. preparation method according to claim 1, which is characterized in that in the step S4, extraction conditions is succinic acid two
Different dioctyl sodium sulfosuccinate (AOT)-isooctane building reverse micelle system, the reverse micelle system is mainly by 30 ~ 50mmol/L's
AOT- isooctane (v:v=3:1) solution and isometric 50 ~ 100mmol/L NaCl and 5 ~ 15mmol/L guanidine hydrochloride solution that contains mix
Close solution composition.
9. preparation method according to claim 8, which is characterized in that the extracting process is longan pulp polyoses extract
According to 0.5 ~ 2(mg/mL) ratio reverse micelle system is added after, after 200 ~ 300rpm vibrates 0.5 ~ 2h, stratification is collected anti-
Micellar phase；Contain 100 ~ 200mmol/L NaCl and 0.1 ~ 0.2 mol/L hydrochloric acid by the reverse micelle phase of collection and in equal volume again
The aqueous solution of guanidine mixes, and after 200 ~ 300rpm vibrates 0.5 ~ 2h, stratification collects water phase, obtains extract liquor.
10. described in any item preparation methods according to claim 1 ~ 9, which is characterized in that in the step S5, condition of dialysing
Are as follows: using shutting off, the bag filter that molecular weight is 3000 ~ 8000Da is dialysed 2 ~ 4 days at 0 ~ 4 DEG C.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN112971064A (en) * | 2021-02-23 | 2021-06-18 | 中国热带农业科学院农产品加工研究所 | Longan pulp superfine powder and preparation method thereof |
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Application publication date: 20181207 |