CN108911154B - Method for biologically treating boron-containing wastewater and improving microalgae grease yield - Google Patents
Method for biologically treating boron-containing wastewater and improving microalgae grease yield Download PDFInfo
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- CN108911154B CN108911154B CN201810778205.0A CN201810778205A CN108911154B CN 108911154 B CN108911154 B CN 108911154B CN 201810778205 A CN201810778205 A CN 201810778205A CN 108911154 B CN108911154 B CN 108911154B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/108—Boron compounds
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Abstract
The invention relates to the field of sewage treatment, and provides a method for biologically treating boron-containing wastewater and improving the yield of microalgae grease. The method utilizes the microalgae to treat the wastewater containing boron, the removal rate of the boron can reach 78% only after about 8 days, the removal efficiency of the boron is high, and the boron removal capacity of the microalgae can reach 3.9 mg/g. The microalgae adopted by the invention does not need artificial domestication, and has low cost, safety and environmental protection. Meanwhile, the method can effectively improve the oil yield in the microalgae while treating the boron-containing wastewater, and the oil yield of the microalgae obtained by the method can be improved by about 77 percent.
Description
Technical Field
The invention relates to the field of sewage treatment, in particular to a method for biologically treating boron-containing wastewater and improving the yield of microalgae grease.
Background
Boron-containing wastewater generally exists in industrial production, such as borate industry, catalytic material preparation, fertilizer production, coal mining, nuclear reactor cooling water, landfill leachate and the like, and the boron concentration is as high as 50mg/L or even more than 100 mg/L. Boron is a trace element and plays an important role in the life activities of animals and plants, but excessive boron can inhibit the cell division and chlorophyll synthesis of plants, thereby influencing the photosynthesis; causing cardiovascular, neurological and digestive system diseases in animals and humans, and harming the reproductive system. The boron concentration required by the wastewater discharge standard is less than 2.4mg/L, and the limit value of the boron concentration required by the drinking water standard established by the world health organization is 0.5 mg/L. With the understanding of boron toxicity and the development of related industries, boron removal methods are attracting attention.
At present, the treatment of boron-containing wastewater is mainly based on a physicochemical method, such as: chemical precipitation, ion exchange resin, reverse osmosis, electrocoagulation, adsorption, etc., or combination of multiple methods. The above treatment method requires consumption of a large amount of chemical reagents, electric energy, membrane materials, adsorbents, etc., and therefore, boron treatment cost is high, and is not suitable for large-scale practical application. Therefore, it is necessary to develop a new boron wastewater treatment technology to solve the problems of the existing treatment methods.
Disclosure of Invention
The invention aims to solve the problems that the treatment cost of the boron-containing wastewater is high and the boron-containing wastewater is not suitable for large-scale application in the prior art, and provides a method for biologically treating the boron-containing wastewater and improving the oil yield of microalgae.
In order to solve the above problems, the present invention provides the following technical solutions:
the invention provides a method for biologically treating boron-containing wastewater and improving microalgae grease yield, which comprises the following steps: controlling the boron concentration in the boron-containing wastewater to be less than or equal to 50mg/L, and inoculating microalgae for biological treatment.
Preferably, the microalgae is selected from chlorella, scenedesmus, diatoms, chrysophyceae or botryococcus braunii.
Preferably, the biomass of the microalgae in the inoculated boron-containing wastewater is more than 0.1 g/L.
Preferably, the biological treatment temperature is 20-28 ℃.
Preferably, the biological treatment time is 7-10 days.
Preferably, during the biological treatment, an exogenous carbon source is added into the boron-containing wastewater, and the final concentration of the exogenous carbon source is 5-20 g/L.
Preferably, the exogenous carbon source is selected from one or more of glucose, sodium acetate, sucrose, maltose and molasses.
Compared with the prior art, the technical scheme provided by the invention has the following advantages:
the invention provides a method for biologically treating boron-containing wastewater and improving microalgae grease yield, which comprises the following steps: controlling the boron concentration in the boron-containing wastewater to be less than or equal to 50mg/L, and inoculating microalgae for biological treatment. The method utilizes the microalgae to treat the wastewater containing boron, the removal rate of the boron can reach 78% only after about 8 days, the removal efficiency of the boron is high, and the boron removal capacity of the microalgae can reach 3.9 mg/g. The invention utilizes the property that microalgae can efficiently remove boron to treat the boron-containing wastewater, and has low cost, safety and environmental protection. The microalgae adopted by the invention does not need artificial domestication, any microalgae can realize the technical scheme of the invention, and the source is simple and convenient.
Meanwhile, the method can effectively improve the oil yield in the microalgae while treating the boron-containing wastewater, when the microalgae is subjected to undesirable external stimulation, the microalgae tends to synthesize and store more oil to resist adverse environments, high-concentration boron is used as an environmental stress condition to promote the oil synthesis and accumulation processes of microalgae cells, and the effects of efficiently removing boron and increasing the oil yield of the microalgae are achieved. The oil yield of the microalgae cultivated by the method can be improved by about 77 percent.
The research of the invention shows that during the treatment of the boron-containing wastewater by using the microalgae, the microalgae in the boron-containing wastewater gradually turns green, and the capability of the microalgae in removing boron elements is possibly related to chlorophyll to a certain extent.
Detailed Description
The invention provides a method for biologically treating boron-containing wastewater and improving microalgae grease yield, which comprises the following steps: controlling the boron concentration in the boron-containing wastewater to be less than or equal to 50mg/L, and inoculating microalgae for biological treatment.
In the invention, the boron concentration in the boron-containing wastewater is preferably controlled to be 10-50 mg/L, and more preferably 25-45 mg/L.
The research of the invention finds that the microalgae has stronger boron element tolerance capability, and can effectively absorb boron into the algae body in the boron-containing wastewater with higher concentration (below 50 mg/L), thereby realizing the high-efficiency removal of boron in the boron-containing wastewater. Experiments show that the efficiency of removing the boron-containing wastewater by using the microalgae can reach 78 percent, while the boron removal efficiency of conventional biological treatment of the boron-containing wastewater is only about 35 percent, namely the boron removal efficiency of the microalgae is high.
Preferably, the microalgae rich in microalgae is selected from microalgae with oil content higher than 14% (mass fraction) such as chlorella, scenedesmus, diatom, chrysophyceae or botryococcus braunii; the invention more preferably relates to oil-rich chlorella which has strong tolerance to boron and good adsorption efficiency.
The source of the microalgae is not specially limited, and the microalgae can be directly purchased from commercial ways without specific domestication or improvement. The method has the advantages of simple and easily obtained raw material sources, low cost and easy large-scale popularization.
In the invention, the concentration of the boron element in the boron-containing wastewater is controlled to be less than or equal to 50mg/L, so that the high boron removal effect and the oil production efficiency are obtained. The research of the invention shows that when the concentration of the boron element is less than or equal to 25mg/L, the growth of the microalgae is not inhibited, and the removal capacity of the boron is increased along with the increase of the concentration of the boron element; when the concentration of the boron element exceeds 50mg/L, the growth of the microalgae can be obviously inhibited, but the boron removal capacity of the microalgae with unit mass is still increased along with the increase of the concentration of the boron, the total mass of the removed boron still keeps a higher level, and the aim of effectively removing most of the boron element can be achieved by further prolonging the time. Based on the fact that the growth of microalgae is inhibited due to high-concentration boron, the aim of quickly and efficiently treating the boron-containing wastewater cannot be fulfilled, and therefore the concentration of the boron in the boron-containing wastewater is controlled to be less than or equal to 50 mg/L.
The invention has no special limitation on how to lead the boron concentration in the boron-containing wastewater to be less than or equal to 50mg/L, and the method can be realized by adopting a mode known in the field, such as adding water for dilution or concentration, and the like.
The microalgae can be inoculated when the boron concentration in the boron-containing wastewater is less than or equal to 50mg/L, and the biomass in the boron-containing wastewater after the microalgae is inoculated is preferably more than 0.1g/L, so that the microalgae can quickly play a role in removing boron elements. If the inoculation amount of the microalgae is too low, the microalgae needs to be proliferated for a certain time, so that the treatment time of the boron-containing wastewater is prolonged, and the cost is increased.
Entering a biological treatment stage from the time of inoculating the microalgae. In the invention, the temperature of the biological treatment is preferably 20-28 ℃, and more preferably 25 ℃; the biological treatment time is 7-10 days, and more preferably 8 days.
Microalgae can be autotrophic by photosynthesis using light, and can also be heterotrophic using organic carbon sources. The boron-containing wastewater contains nutrient components such as nitrogen, phosphorus and the like besides high-concentration boron elements, and is used for microalgae to perform heterotrophic reaction. In order to accelerate the treatment efficiency of the boron-containing wastewater, a certain amount of exogenous carbon source is preferably additionally added during biological treatment to supplement, so that the efficiency of microalgae for treating the boron-containing wastewater can be enhanced, and the treatment time can be shortened.
The preferred exogenous carbon source is added into the boron-containing wastewater according to the proportion of 5-20 g/L, and the more preferred concentration is 10-15 g/L. The exogenous carbon source in the present invention includes, but is not limited to, glucose, sodium acetate, sucrose, maltose and molasses, and may be one or a combination of carbon sources, and the present invention is not particularly limited thereto.
The method disclosed by the invention is applied to improving the yield of the microalgae grease. The high concentration boron in the boron-containing wastewater can be used as adverse stimulation to the microalgae, and the microalgae tends to synthesize more grease under the adverse stimulation to resist adverse environment, so that the method can simultaneously realize the high-efficiency removal of boron and the improvement of the grease yield of the microalgae.
In order to further illustrate the present invention, the following embodiments are described in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
BG11 medium based, B concentration (BO)3 3--B) was 0.5mg/L, the initial biomass of the strain was 0.1g/L by adding Chlorella (Chlorella regularis var. minima) to the strain, and the heterotrophic culture was carried out at 25 ℃ by adding 10g/L of glucose as a carbon source.
The biomass gradually increases in the culture process, and tends to be stable after 3 days, the maximum biomass is reached after 6 days of culture, the maximum biomass is 3.8g/L, the boron removal rate reaches 78%, and the boron removal capacity of the chlorella is 0.1 mg/g. The oil content is 20 percent, and the oil yield is 0.7 g/L.
The boron removal capability of the chlorella refers to the mass of boron which can be removed by the chlorella per unit mass in the treatment period of the wastewater containing boron. The calculation formula is as follows:
boron removal capacity (mg/g) — [ initial boron concentration (mg/L) -boron concentration after treatment (mg/L) ]/[ microalgae concentration after treatment (g/L) -initial microalgae concentration (g/L) ]
Example 2
Adjusted on the basis of BG11 medium, with B concentration (BO)3 3--B) was 10mg/L, the initial biomass of the test strain was 0.1g/L by adding Chlorella angularis var. minima (purchased from fresh water algae Bank, Chinese academy of sciences) and the heterotrophic culture was carried out at 25 ℃ by adding 10g/L glucose as a carbon source.
The biomass gradually increases in the culture process, and tends to be stable after 3 days, the maximum biomass is reached after 6 days of culture, the maximum biomass is 3.8g/L, the boron removal rate reaches 34%, and the boron removal capacity of the chlorella is 1.0 mg/g. The oil content is 23 percent, and the oil yield is 0.9 g/L. The growth of microalgae is not affected basically, and compared with example 1, the biomass is not changed obviously, the boron removal capacity is improved by 9 times, the oil content is improved by 15%, and the oil yield is improved by 29%.
Example 3
Adjusted on the basis of BG11 medium, with B concentration (BO)3 3--B) was 25mg/L, the initial biomass of the strain was 0.1g/L by adding Chlorella angularis to the strain, and the heterotrophic culture was carried out at 25 ℃ by adding 10g/L of glucose as a carbon source.
The biomass gradually increases in the culture process, and tends to be stable after 3 days, the maximum biomass is reached after 6 days of culture, the maximum biomass is 3.9g/L, the boron removal rate reaches 33%, and the boron removal capacity of the chlorella is 2.8 mg/g. The oil content is 34 percent, and the oil yield is 1.3 g/L. The growth of microalgae is not affected basically, and compared with the example 1, the biomass is increased slightly by 3 percent; the boron removal capacity is improved by 27 times, the oil content is improved by 70 percent, and the oil yield is improved by 86 percent.
Example 4
Adjusted on the basis of BG11 medium, where B is concentratedDegree (in BO)3 3--B) was 50mg/L, the initial biomass of the strain was 0.1g/L by adding Chlorella angularis to the strain, and the heterotrophic culture was carried out at 25 ℃ by adding 10g/L glucose as a carbon source.
The biomass gradually increases in the culture process, and tends to be stable after 3 days, the maximum biomass is reached after 6 days of culture, the maximum biomass is 1.9g/L, the boron removal rate reaches 14%, and the boron removal capacity of the chlorella is 3.9 mg/g. The oil content is 37 percent, and the oil yield is 0.7 g/L. The growth of microalgae is influenced to a certain extent, and compared with the example 1, the biomass is reduced by 50%; the boron removal capacity is improved by 38 times, the oil content is improved by 85 percent, and the oil yield is not obviously increased.
Through comparison of the embodiments 1 to 4, the boron removal capability of the microalgae is directly related to the boron concentration within the range that the boron concentration is less than or equal to 50mg/L, and the higher the boron concentration is, the stronger the boron removal capability of the microalgae per unit mass is, and the maximum boron removal capability reaches 3.9 mg/g. Along with the increase of the boron concentration, the oil content of the microalgae gradually increases to reach 37 percent at most. When the boron concentration is less than or equal to 25mg/L, the growth of the microalgae is basically not influenced, when the boron concentration is increased to 50mg/L, the growth of the microalgae is obviously inhibited, but the higher boron removal capability can be still kept, which indicates that the tolerance concentration of the microalgae to boron can reach 50mg/L at most.
The above examples show that the technology of removing boron by using microalgae and simultaneously producing biodiesel is feasible, the concentration of boron in the treated wastewater can be as high as 50mg/L, and the boron removal capacity of the microalgae can be as high as 3.9 mg/g. The oil content of the microalgae can be improved to 37 percent, and the oil yield can reach 1.3g/L at most. In the embodiment, microalgae species are not domesticated, and the boron removal efficiency and the oil yield can be further improved by means of algae species domestication, inoculation concentration improvement, culture condition optimization and the like.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A method for biologically treating boron-containing wastewater and improving microalgae grease yield comprises the following steps: controlling the boron concentration in the boron-containing wastewater to be 25-50 mg/L, and inoculating microalgae for biological treatment; the microalgae is selected from Chlorella, Scenedesmus, diatom, Chrysophytum or Botryococcus braunii; during biological treatment, adding an exogenous carbon source into the boron-containing wastewater, wherein the final concentration of the exogenous carbon source is 5-20 g/L; the biological treatment time is 7-10 days.
2. The method for biologically treating boron-containing wastewater and improving microalgae oil yield according to claim 1, wherein the biomass of microalgae in the inoculated boron-containing wastewater is greater than 0.1 g/L.
3. The method for biologically treating boron-containing wastewater and improving microalgae oil yield according to claim 1, wherein the biological treatment temperature is 20-28 ℃.
4. The method for biologically treating boron-containing wastewater and improving microalgae lipid yield according to claim 1, wherein the exogenous carbon source is selected from one or more of glucose, sodium acetate, sucrose, maltose and molasses.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955846A (en) * | 2010-10-15 | 2011-01-26 | 哈尔滨工业大学 | Production method of microalgae grease based on low-carbon emission recycling of domestic sewage |
| CN104630295A (en) * | 2015-03-08 | 2015-05-20 | 中国海洋大学 | Method for treating municipal secondary wastewater and producing grease by using immobilized microalgae |
| CN105132351A (en) * | 2015-10-12 | 2015-12-09 | 山东大学 | Method for rapidly accumulating lipid by high-temperature stress on micro-algae |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955846A (en) * | 2010-10-15 | 2011-01-26 | 哈尔滨工业大学 | Production method of microalgae grease based on low-carbon emission recycling of domestic sewage |
| CN104630295A (en) * | 2015-03-08 | 2015-05-20 | 中国海洋大学 | Method for treating municipal secondary wastewater and producing grease by using immobilized microalgae |
| CN105132351A (en) * | 2015-10-12 | 2015-12-09 | 山东大学 | Method for rapidly accumulating lipid by high-temperature stress on micro-algae |
Non-Patent Citations (2)
| Title |
|---|
| Boron bioremoval by a newly isolated Chlorella sp. and its stimulation by growth stimulators;Burcu Ertit Tastan等;《Water Research》;20111028;第167-175页 * |
| 元素磷在氮缺乏刺激微藻生物柴油产率提高的作用研究;储菲菲;《中国博士学位论文全文数据库 工程科技I辑》;20141015;第9-15页 * |
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