CN108905630A - A kind of rapid serum extractor - Google Patents
A kind of rapid serum extractor Download PDFInfo
- Publication number
- CN108905630A CN108905630A CN201811003529.3A CN201811003529A CN108905630A CN 108905630 A CN108905630 A CN 108905630A CN 201811003529 A CN201811003529 A CN 201811003529A CN 108905630 A CN108905630 A CN 108905630A
- Authority
- CN
- China
- Prior art keywords
- blood
- serum
- cylinder type
- hollow shell
- separation membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000002966 serum Anatomy 0.000 title claims abstract description 49
- 210000004369 blood Anatomy 0.000 claims abstract description 71
- 239000008280 blood Substances 0.000 claims abstract description 71
- 239000012528 membrane Substances 0.000 claims abstract description 45
- 238000000926 separation method Methods 0.000 claims abstract description 38
- 238000005070 sampling Methods 0.000 claims abstract description 20
- 238000007789 sealing Methods 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims description 18
- 239000000919 ceramic Substances 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 239000011347 resin Substances 0.000 claims description 4
- 229920005989 resin Polymers 0.000 claims description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
- 239000010935 stainless steel Substances 0.000 claims description 4
- 239000004695 Polyether sulfone Substances 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 229920006393 polyether sulfone Polymers 0.000 claims description 3
- -1 polyethylene Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 239000002041 carbon nanotube Substances 0.000 claims description 2
- 229910021393 carbon nanotube Inorganic materials 0.000 claims description 2
- 229960003638 dopamine Drugs 0.000 claims description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 2
- 239000010931 gold Substances 0.000 claims description 2
- 229910052737 gold Inorganic materials 0.000 claims description 2
- 229910021389 graphene Inorganic materials 0.000 claims description 2
- 229910052751 metal Inorganic materials 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- 229920002492 poly(sulfone) Polymers 0.000 claims description 2
- 229920000128 polypyrrole Polymers 0.000 claims description 2
- 229910052709 silver Inorganic materials 0.000 claims description 2
- 239000004332 silver Substances 0.000 claims description 2
- 229910052573 porcelain Inorganic materials 0.000 claims 1
- 102000008946 Fibrinogen Human genes 0.000 abstract description 6
- 108010049003 Fibrinogen Proteins 0.000 abstract description 6
- 229940012952 fibrinogen Drugs 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000000605 extraction Methods 0.000 abstract description 3
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 6
- 210000001772 blood platelet Anatomy 0.000 description 5
- 210000003743 erythrocyte Anatomy 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000000306 component Substances 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 206010018910 Haemolysis Diseases 0.000 description 2
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 229920001800 Shellac Polymers 0.000 description 1
- 230000001194 anti-hemostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003918 blood extract Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 102000005525 fibrillarin Human genes 0.000 description 1
- 108020002231 fibrillarin Proteins 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- ZLGIYFNHBLSMPS-ATJNOEHPSA-N shellac Chemical compound OCCCCCC(O)C(O)CCCCCCCC(O)=O.C1C23[C@H](C(O)=O)CCC2[C@](C)(CO)[C@@H]1C(C(O)=O)=C[C@@H]3O ZLGIYFNHBLSMPS-ATJNOEHPSA-N 0.000 description 1
- 229940113147 shellac Drugs 0.000 description 1
- 235000013874 shellac Nutrition 0.000 description 1
- 239000004208 shellac Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
Abstract
The present invention relates to a kind of rapid serum extractors, it is characterised in that is made of cylinder type hollow shell (1), sealing piston (2), sampling head (3) and blood separation membrane piece (4);Wherein cylinder type hollow shell (1) is serum collection main body, and the upper end is inserted by sealing piston (2) and guarantees air-tightness, and lower end is connected with sampling head (3);Blood separation membrane piece (4) is fixed on cylinder type hollow shell (1) bottom.Utilize the technology of UF membrane, vacuum power is generated by sampling head (3) by drawing sealing piston (2), and whole blood is pumped into cocurrent through blood Separation membrane (4), using Separation membrane (4) nanoscale duct in blood haemocyte and fibrinogen shut off, only serum is allowed to reach cylinder type hollow shell (1) through diaphragm, completes the acquisition of serum.The apparatus structure is simple, and it is convenient to operate, and can realize the extraction of pure serum in whole blood as quick as thought, substantially increase the efficiency of medical diagnosis on disease in hospital, provides the technological means of a kind of blood preseration and sample backtracking.
Description
Technical field
The present invention relates to a kind of rapid serum extractor, which uses seperation film for main element, passes through the sieve of film
It is allocated as with being completely removed leucocyte, red blood cell, blood platelet and the fibrinogen in whole blood and do not destroy eucaryotic cell structure to mention
Pure serum is taken out, the analysis and long term storage for blood.
Background technique
In modern medical service, the inspection of blood is one of most important technology of clinical diagnosis.Generally, due to the self-solidifying of blood
Mechanism, relevant biochemical analysis are required to extract serum from blood as analysis sample, meanwhile, serum after extraction compared to
Whole blood, storage time will greatly extend, the backtracking of convenience sample.Currently, the serum separation method generally used in hospital is
Each component is layered by different revolving speeds and is collected based on the quality difference of each component in blood by centrifugal filtration.However, blood
Haemocyte in liquid is very fragile, and the breakage of cell wall easily occurs at high speed, causes haemolysis, but the slow-speed of revolution
It is difficult for each group to be kept completely separate again.Therefore, quick, the lossless and thorough serum extractive technique of separation will be real with important medical treatment
Trample meaning and large market prospect.
Membrane separation technique is a kind of emerging isolation technics in situ, and efficiently, the characteristic of low energy consumption and low cost becomes
The Hot spots for development of separation field at present.It is applied at present in the existing part commercialization achievement of medical field, such as Rend dialysis.So
And the use of most of film is required based on finished Components and device, at present on the market still not accordingly based on membrane technology
Serum extraction element.As can developing a kind of inexpensive, high-throughput membrane type serum extractor, then traditional centrifugation skill can be solved
The quick nondestructive of the defects of time-consuming for art, easy haemolysis, the separation in situ and serum of realizing blood extracts, and dramatically speeds up blood testing
Period improves the accuracy of detection.
Summary of the invention
The object of the present invention is to provide a kind of rapid serum extractor, a kind of serum of the rapidly extracting serum from whole blood is mentioned
Device is taken, to improve the long-term preservability of the efficiency of blood testing and blood sample in clinical treatment.The serum extractor structure is simple, at
This it is lower, it can be achieved that manually and be automatically brought into operation, whole blood sample is separated immediately suitable for blood collection procedure, remove blood in
Red blood cell, leucocyte, blood platelet and fibrinogen, extract pure serum.The device is easy to operate, it is easy to clean and
Disinfection has good medical prospect.
The technical scheme is that:A kind of rapid serum extractor, it is characterised in that by cylinder type hollow shell 1, close
Piston 2, sampling head 3 and blood separation membrane piece 4 is sealed to form;Wherein cylinder type hollow shell 1 be serum collection main body, the upper end by
Sealing piston 2 is inserted into and guarantees air-tightness, and lower end is connected with sampling head 3;Blood separation membrane piece 4 is fixed on cylinder type hollow shell
The diameter of 1 bottom of body, blood separation membrane piece 4 is identical as the internal diameter of cylinder type hollow shell 1, it is ensured that it is closely stuck in shell bottom
Portion.Vacuum power is generated by sampling head by drawing sealing piston, whole blood is pumped into cocurrent through blood Separation membrane, utilizes seperation film
Piece nanoscale duct in blood haemocyte and fibrinogen shut off, only allow serum through diaphragm reach cylinder type hollow
Shell completes the acquisition of serum.
It is preferred that the material of the cylinder type hollow shell 1 is glass, plastics, resin or ceramics.The cylindrical empty
1 diameter of heart shell is 1-3cm, length 4-10cm;The sealing piston 2 is matched with 1 size of cylinder type hollow shell to protect
Air-tightness is demonstrate,proved, while can be plugged up and down.
It is preferred that the material of sampling head 3 is metal, glass, plastics, resin or ceramics.It is preferred that the material of blood separation membrane piece 4
For:Ceramics, stainless steel, gold, silver, polysulfones, polyether sulfone, polyethylene, polypyrrole, dopamine, graphene or carbon nanotube.Sampling head
3 are connected 1 with cylinder type hollow shell.
It is preferred that the diameter of blood separation membrane piece 4 is 1-3cm, with a thickness of 0.1-1cm.4 structure of blood separation membrane piece is chip
Film, membrane aperture 100-500nm.Blood separation membrane piece 4 is fixed on cylinder type hollow shell lower end, not mobile with sealing piston.
The extractor can be using the technology of UF membrane to red blood cell, leucocyte, blood platelet and the fiber in fresh blood
Proteinogen shuts off, and only allows serum through the duct of film and is collected, and the rapid serum that can be used for clinical blood chemical examination mentions
It takes and the long-time storage of blood sample.When work, make to generate negative pressure formation draft in shell by the twitch of sealing piston, it will
Blood sucks from sampling head and contacts blood parting surface membrane surface, since the pore size of Separation membrane will be lower than haemocyte and fibre
Fibrillarin is former, sieves principle according to size, these blood constitutents are trapped in membrane surface, and smaller serum then can be fast
Speed penetrates fenestra, reaches enclosure interior and is collected storage.
Beneficial effect:
The present invention is based on membrane separation technique, a kind of quick, lossless serum extractor is designed and prepared.The device is with blood
Seperation film is core component, by nanoscale UF membrane duct by red blood cell, leucocyte, blood platelet and the fiber egg in whole blood
White original is all shut off, and pure serum is only allowed to be collected by fenestra road.The apparatus structure is simple, operation is convenient, and by
In in situ, the lossless stalling characteristic using film, can anti-Hemostatic Oral Liquid cause the breakage of haemocyte, obtained blood during the separation process
Pure cleanliness is high, pollution-free, substantially increases the efficiency of medical diagnosis on disease in hospital, provides a kind of blood preseration and sample backtracking
Technological means.
Detailed description of the invention
Fig. 1 is a kind of structure drawing of device of rapid serum extractor, in figure:1- cylinder type hollow shell;2- sealing piston;
3- sampling head;4- blood separation membrane piece.
Specific embodiment
Embodiment 1
As shown in Figure 1:A kind of rapid serum extractor, by cylinder type hollow shell 1, sealing piston 2, sampling head 3 and blood
Liquid Separation membrane 4 forms;Wherein cylinder type hollow shell 1 is serum collection main body, and the upper end is inserted into and is guaranteed by sealing piston 2
Air-tightness, lower end are connected with sampling head 3;Blood separation membrane piece 4 is fixed on 1 bottom of cylinder type hollow shell, blood separation membrane
The diameter of piece 4 is identical as the internal diameter of cylinder type hollow shell 1, it is ensured that it is closely stuck in housing bottom.Pass through drawing sealing piston
Vacuum power is generated by sampling head, whole blood is pumped into cocurrent through blood Separation membrane, since the size of haemocyte in blood is all larger than 2 μ
M, thus using Separation membrane nanoscale duct in blood haemocyte and fibrinogen shut off, only allow serum penetrate film
Piece reaches cylinder type hollow shell, completes the acquisition of serum.
The working method of the serum extractor is sealing piston 2 to be inserted into cylinder type hollow shell 1, and be pushed into bottom, will
In the new blood that the insertion of sampling head 3 is extracted, drawing sealing piston 2 generates negative pressure, and blood is pumped into cylinder type hollow shell 1,
And contact blood separation membrane piece 4 in blood haemocyte and fibrinogen shut off, remaining serum passed through blood
Separation membrane 4 reaches storage in 1 cavity of cylinder type hollow shell.
Embodiment 2
The device of the invention is with embodiment 1, to the preferred ruler of one of which of blood separated in synchronization verifying attachment of the invention
Very little and material is described in detail.The material of cylinder type hollow shell 1 is glass, a diameter of 1cm, length 4cm;Sampling head
3 material is stainless steel;The material of blood separation membrane piece 4 is aluminium oxide, diameter 1cm, with a thickness of 0.1cm.Blood separation membrane
4 structure of piece is chip film, membrane aperture 500nm.
Embodiment 3
The device of the invention is with embodiment 1, to the preferred ruler of one of which of blood separated in synchronization verifying attachment of the invention
Very little and material is described in detail.The material of cylinder type hollow shell 1 is shellac, a diameter of 3cm, length 10cm;Sampling
First 3 material is zirconia ceramics;The material of blood separation membrane piece 4 is polyether sulfone, diameter 3cm, with a thickness of 1cm.Blood point
It is chip film, membrane aperture 10nm from 4 structure of diaphragm.
Embodiment 4
The device of the invention is with embodiment 1, to the preferred ruler of one of which of blood separated in synchronization verifying attachment of the invention
Very little and material is described in detail.The material of cylinder type hollow shell 1 is aluminium oxide ceramics, a diameter of 2cm, length 6cm;
The material of sampling head 3 is polyethylene;The material of blood separation membrane piece 4 is stainless steel, diameter 2cm, with a thickness of 0.5cm.Blood
4 structure of Separation membrane is chip film, membrane aperture 100nm.
Above-described embodiment 2-4, using whole blood as raw material, it is as follows that the serum extracted extracts performance:
Red blood cell content in serum | 0 |
Leucocyte content in serum | 0 |
Platelet content in serum | 0 |
Hemoglobin content in serum | 0g/L |
Detect relative standard deviation | <5% |
Four embodiments described above are only the preferred embodiment of the present invention, before not departing from the technology of the present invention principle
It puts, being correspondingly improved and replacing also should be regarded as protection scope of the present invention.
Claims (7)
1. a kind of rapid serum extractor, it is characterised in that by cylinder type hollow shell (1), sealing piston (2), sampling head (3)
It is formed with blood separation membrane piece (4);Wherein cylinder type hollow shell (1) is serum collection main body, and the upper end is by sealing piston (2)
It is inserted into and guarantees air-tightness, lower end is connected with sampling head (3);Blood separation membrane piece (4) is fixed on cylinder type hollow shell (1)
Bottom.
2. rapid serum extractor according to claim 1, it is characterised in that:The cylinder type hollow shell (1)
Material is glass, plastics, resin or ceramics.
3. rapid serum extractor according to claim 1, it is characterised in that:The cylinder type hollow shell (1) is straight
Diameter is 1-3cm, length 4-10cm.
4. rapid serum extractor according to claim 1, it is characterised in that:The material of sampling head (3) is metal, glass
Glass, plastics, resin or ceramics.
5. rapid serum extractor according to claim 1, it is characterised in that:The material of blood separation membrane piece (4) is:Pottery
Porcelain, stainless steel, gold, silver, polysulfones, polyether sulfone, polyethylene, polypyrrole, dopamine, graphene or carbon nanotube.
6. rapid serum extractor according to claim 1, it is characterised in that:The diameter of blood separation membrane piece (4) is 1-
3cm, with a thickness of 0.1-1cm.
7. blood separated in synchronization verifying attachment according to claim 1, it is characterised in that:Blood separation membrane piece (4) structure
For chip film, membrane aperture 100-500nm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811003529.3A CN108905630A (en) | 2018-08-30 | 2018-08-30 | A kind of rapid serum extractor |
Applications Claiming Priority (1)
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CN201811003529.3A CN108905630A (en) | 2018-08-30 | 2018-08-30 | A kind of rapid serum extractor |
Publications (1)
Publication Number | Publication Date |
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CN108905630A true CN108905630A (en) | 2018-11-30 |
Family
ID=64406390
Family Applications (1)
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CN201811003529.3A Pending CN108905630A (en) | 2018-08-30 | 2018-08-30 | A kind of rapid serum extractor |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0763769A (en) * | 1993-08-24 | 1995-03-10 | Asahi Fiber Glass Co Ltd | Method and device for removing solid component from humor |
JP2004344874A (en) * | 2002-11-19 | 2004-12-09 | Sekisui Chem Co Ltd | Separation membrane of plasma or serum and filter apparatus using separation membrane of plasma or serum |
CN1714292A (en) * | 2002-11-19 | 2005-12-28 | 积水化学工业株式会社 | Plasma or serum separation membrane and filter apparatus including the plasma or serum separation membrane |
JP2007333578A (en) * | 2006-06-15 | 2007-12-27 | Sekisui Chem Co Ltd | Blood separation member |
CN105307758A (en) * | 2013-06-27 | 2016-02-03 | 曼·胡默尔有限公司 | Ceramic whole blood hollow fiber membrane filter medium and use thereof for separating blood plasma / serum from whole blood |
CN209549188U (en) * | 2018-08-30 | 2019-10-29 | 南京工业大学 | A kind of rapid serum extractor |
-
2018
- 2018-08-30 CN CN201811003529.3A patent/CN108905630A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0763769A (en) * | 1993-08-24 | 1995-03-10 | Asahi Fiber Glass Co Ltd | Method and device for removing solid component from humor |
JP2004344874A (en) * | 2002-11-19 | 2004-12-09 | Sekisui Chem Co Ltd | Separation membrane of plasma or serum and filter apparatus using separation membrane of plasma or serum |
CN1714292A (en) * | 2002-11-19 | 2005-12-28 | 积水化学工业株式会社 | Plasma or serum separation membrane and filter apparatus including the plasma or serum separation membrane |
JP2007333578A (en) * | 2006-06-15 | 2007-12-27 | Sekisui Chem Co Ltd | Blood separation member |
CN105307758A (en) * | 2013-06-27 | 2016-02-03 | 曼·胡默尔有限公司 | Ceramic whole blood hollow fiber membrane filter medium and use thereof for separating blood plasma / serum from whole blood |
CN209549188U (en) * | 2018-08-30 | 2019-10-29 | 南京工业大学 | A kind of rapid serum extractor |
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