CN108902793A - A kind of preparation method of snakehead enzymolysis liquid - Google Patents
A kind of preparation method of snakehead enzymolysis liquid Download PDFInfo
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- CN108902793A CN108902793A CN201810966209.1A CN201810966209A CN108902793A CN 108902793 A CN108902793 A CN 108902793A CN 201810966209 A CN201810966209 A CN 201810966209A CN 108902793 A CN108902793 A CN 108902793A
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- snakehead
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/70—Comminuted, e.g. emulsified, fish products; Processed products therefrom such as pastes, reformed or compressed products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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Abstract
The invention discloses a kind of preparation methods of snakehead enzymolysis liquid, the present invention is by discarding internal organ and head for snakehead, after blending, after lactic acid immersion is added, lactic acid enters inside structure of fish muscle, hydrogenated fat is conducive to protein leaching, improves the hydrolysis result of protease, lecithin is added after enzymatic hydrolysis, after being combined with the fat of hydrogenation, the cyclic annular conjugate easily in conjunction with water is formed, is conducive to enzymolysis liquid filtering;The high enzymolysis liquid of available a kind of nitrogen content through the invention, and filtering is smooth, does not need that lipase is added, while this method preparation process is simple, is suitable for industrialization and large-scale production.
Description
Technical field
The present invention relates to a kind of preparation methods of snakehead enzymolysis liquid, belong to field of health care products.
Background technique
Snakehead(Ophiolephalus argus Cantor)It is commonly called as snakeheaded fish, is a kind of river for being distributed widely in China various regions
River, lake, Gou Tang, the carnivorous freshwater fish in pond.Its fine and tender taste, spur is few, delicious flavour, and dressed fish is high, albumen
Matter content is high, is known as the title of " treasure in fish ", is the high-class healthy product that a kind of nutrition is comprehensive, meat flavour is delicious, and one to being considered as disease
The nourishing treasure of rehabilitation and old young void person afterwards.
The protein content of snakehead is higher, and up to 19.5 grams, fat content is less, and calcium content is higher than the content of other fishes, also
Contain multivitamin and inorganic salts.Snakeheaded fish also contains more than the 10 kinds of free amino acids such as citrulling, serine, methionine, these
Amino acid is of great significance to enhancing body resistance against diseases.Also have wind-dispelling control infantile malnutrition due to digestive disturbances or intestinalparasites, invigorate the spleen and benefit qi, Li Shui disappears
The benefits of swollen, is suitable in poor health, Hypoproteinemia, deficiency of spleen-QI and stomach-QI, malnutrition, and the people of anaemia is edible, and western wide one with civil
It regards snakeheaded fish often as precious tonic, to stimulating milk secretion, enriches blood.Clinically it is usually used in stasis eliminatings raw new, nourishing maintenance, the medical treatment of invigorating spleen for diuresis
Effect is eaten after being ill, after postpartum and operation, have the function of myogenic enrich blood, healing acceleration wound, oedema, wet can also be treated
The diseases such as numbness, tinea pedis, hemorrhoid, scabies.
The nutrients of snakehead is mainly obtained by protease hydrolyzed mode.It can will be in snakehead by protease hydrolyzed
Protein macromolecule resolves into the amino acid of small molecule, has very high nutritive value and medical functions, however due in snakehead
Fat constituent is high, its enzymolysis liquid oiliness is high after enzymatic hydrolysis, filtration difficulty, frequently by a certain amount of lipase of addition in practical operation
After enzymatic hydrolysis fat, refilter, due to lipase and a kind of protein can by protease hydrolyzed, and the specificity of lipase compared with
By force, all lipoid materials cannot be decomposed, even if lipase is added, filter effect is still poor, for these reasons originally
A kind of preparation method of snakehead enzymolysis liquid is developed in invention, and not needing addition its enzymolysis liquid of lipase smooth can filter.
Summary of the invention
To solve the above-mentioned problems of the prior art, the present invention provides a kind of preparation method of snakehead enzymolysis liquid, this hair
Bright preparation method is simply controllable, is suitable for industrialization and large-scale production, and does not need that lipase is added.
In order to realize that foregoing invention, embodiment are as follows:
(One)Take fresh snakehead meat into enzymatic vessel, addition is equivalent to fresh 2-4 times of the snakehead meat water measured, and stirs, and lactic acid, heating is added
It is 40-60 DEG C to temperature, constant temperature is kept for 4-8 hours, and alkali regulator is added, and adjusting environmental system pH is 7-9.
Preferably, the fresh snakehead meat is the muddy flesh that after snakehead decaptitating and internal organ, will be blended by pulper.
Preferably, the lactic acid additional amount is the 1-5% of fresh snakehead meat quality.It is preferred that 3%.
Preferably, the alkali regulator is sodium hydroxide, potassium hydroxide, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium carbonate
One of or several compositions, preferred sodium hydroxide.
(Two)Protease is added, digests 3-5 hours, heating is boiled 5-15 minutes, is cooled to 20-40 DEG C, lecithin is added,
Constant temperature is kept for 5-15 hours, and filtering obtains enzymolysis liquid.
Preferably, the proteinase activity is greater than 2500 units/g.
Further, the protease additional amount is the 1-5% of fresh snakehead meat quality;It is preferred that 3%.
Preferably, the lecithin additional amount is the 0.1-0.7% of integral system quality;It is preferred that 0.4%.
The filtering includes tripod pendulum type batch centrifugal(Maximum (top) speed 4000r/min)It is filtered with leaf filter.
The method have the characteristics that:
The present invention is softened fresh snakehead meat by the way that certain lactic acid is added, and lactic acid molecules key in organization internal, decomposites hydrogen ion,
Hydrogenated oil and fat substance enables the protein of organization internal adequately to be digested, and then improves the nitrogen content of final enzymolysis liquid;
Another feature of the present invention is that lecithin is added, and after the oil substances of hydrogenation are in conjunction with the fat-soluble end in lecithin, is formed
Ring-type package molecule, external is the hydrogenation end of the water-wet side of lecithin and grease, and oiliness greatly reduces.
The beneficial effects of the present invention are:
1. the high enzymolysis liquid of available a kind of nitrogen content through the invention.
2. the method for the present invention is simply controllable, it is suitable for industrialization scale.
3. the present invention does not need that lipase enzymatic hydrolysis fat is added, it can smoothly filter, obtain enzymolysis liquid.
Specific embodiment
Main points in order to better understand the present invention are now described in further detail foregoing invention by specific embodiment, but under
Stating embodiment should not be understood as to further limitation of the invention.
Embodiment explanation:
1. all embodiments and comparative example take fresh snakehead 20kg, digested in the enzymatic vessel of 150L.
2. protease potency is 3500 units, MP Biomedicals company is derived from.
3. lipase potency is 300u/mg, from the purple chemical reagent work in Shanghai.
Embodiment 1
A kind of preparation method of snakehead enzymolysis liquid, preparation step are as follows:
1. after snakehead decaptitating and internal organ, the muddy flesh blended by pulper is added the water for being equivalent to 3 times of fresh snakehead meat amounts, stirs
It mixes, lactic acid is added, lactic acid additional amount is the 3% of fresh snakehead meat quality.Being heated to temperature is 50 DEG C, and constant temperature is kept for 6 hours, is added
Alkali regulator, adjusting environmental system pH is 8.
2. protease is added, protease additional amount is the 3% of fresh snakehead meat quality.Enzymatic hydrolysis 4 hours, heating are boiled 10 minutes,
30 DEG C are cooled to, lecithin is added, lecithin additional amount is the 0.6% of integral system quality.Constant temperature is kept for 10 hours, and filtering obtains
Enzymolysis liquid.
Embodiment 2
A kind of preparation method of snakehead enzymolysis liquid, preparation step are as follows:
1. after snakehead decaptitating and internal organ, the muddy flesh blended by pulper is added the water for being equivalent to 2 times of fresh snakehead meat amounts, stirs
It mixes, lactic acid is added, lactic acid additional amount is the 1% of fresh snakehead meat quality.Being heated to temperature is 40 DEG C, and constant temperature is kept for 4 hours, is added
Alkali regulator, adjusting environmental system pH is 7.
2. protease is added, protease additional amount is the 1% of fresh snakehead meat quality.Enzymatic hydrolysis 3 hours, heating are boiled 5 minutes,
20 DEG C are cooled to, lecithin is added, lecithin additional amount is the 0.1% of integral system quality.Constant temperature is kept for 5 hours, and filtering obtains
Enzymolysis liquid.
Embodiment 3
A kind of preparation method of snakehead enzymolysis liquid, preparation step are as follows:
1. after snakehead decaptitating and internal organ, the muddy flesh blended by pulper is added the water for being equivalent to 4 times of fresh snakehead meat amounts, stirs
It mixes, lactic acid is added, lactic acid additional amount is the 5% of fresh snakehead meat quality.Being heated to temperature is 60 DEG C, and constant temperature is kept for 8 hours, is added
Alkali regulator, adjusting environmental system pH is 9.
2. protease is added, protease additional amount is the 5% of fresh snakehead meat quality.Enzymatic hydrolysis 5 hours, heating boil 5-15 points
Clock is cooled to 40 DEG C, and lecithin is added, and lecithin additional amount is the 0.7% of integral system quality.Constant temperature is kept for 15 hours, mistake
Filter, obtains enzymolysis liquid.
Comparative example 1
A kind of preparation method of snakehead enzymolysis liquid, preparation step are as follows:
1. after snakehead decaptitating and internal organ, the muddy flesh blended by pulper is added the water for being equivalent to 3 times of fresh snakehead meat amounts, stirs
It mixes, being heated to temperature is 50 DEG C, and constant temperature is kept for 6 hours, and alkali regulator is added, and adjusting environmental system pH is 8.
2. protease is added, protease additional amount is the 3% of fresh snakehead meat quality.Enzymatic hydrolysis 4 hours, heating are boiled 10 minutes,
30 DEG C are cooled to, lecithin is added, lecithin additional amount is the 0.6% of integral system quality.Constant temperature is kept for 10 hours, and filtering obtains
Enzymolysis liquid.
Comparative example 2
A kind of preparation method of snakehead enzymolysis liquid, preparation step are as follows:
1. after snakehead decaptitating and internal organ, the muddy flesh blended by pulper is added the water for being equivalent to 3 times of fresh snakehead meat amounts, stirs
It mixes, lactic acid is added, lactic acid additional amount is the 3% of fresh snakehead meat quality.Being heated to temperature is 50 DEG C, and constant temperature is kept for 6 hours, is added
Alkali regulator, adjusting environmental system pH is 8.
2. protease is added, protease additional amount is the 3% of fresh snakehead meat quality.Enzymatic hydrolysis 4 hours, heating are boiled 10 minutes,
30 DEG C are cooled to, constant temperature is kept for 10 hours, and filtering obtains enzymolysis liquid.
Comparative example 3
A kind of preparation method of snakehead enzymolysis liquid, preparation step are as follows:
1. after snakehead decaptitating and internal organ, the muddy flesh blended by pulper is added the water for being equivalent to 3 times of fresh snakehead meat amounts, stirs
It mixes, being heated to temperature is 50 DEG C, and constant temperature is kept for 6 hours, and alkali regulator is added, and adjusting environmental system pH is 8.
2. protease is added, protease additional amount is the 3% of fresh snakehead meat quality.Enzymatic hydrolysis 4 hours, heating are boiled 10 minutes,
30 DEG C are cooled to, constant temperature is kept for 10 hours, and filtering obtains enzymolysis liquid.
Comparative example 4
A kind of preparation method of snakehead enzymolysis liquid, preparation step are as follows:
1. after snakehead decaptitating and internal organ, the muddy flesh blended by pulper is added the water for being equivalent to 3 times of fresh snakehead meat amounts, stirs
It mixes, being heated to temperature is 50 DEG C, and constant temperature is kept for 6 hours, and alkali regulator is added, and adjusting environmental system pH is 8.
2. protease and lipase is added, protease additional amount is the 3% of fresh snakehead meat quality, and lipase additional amount is fresh
The 3% of snakehead meat quality digests 4 hours, and heating is boiled 10 minutes, is cooled to 30 DEG C, and filtering obtains enzymolysis liquid.
Situation is filtered to investigate
The filter type of gained enzymolysis liquid is first to cross tripod pendulum type batch centrifugal in above-described embodiment 1-3 and comparative example 1-4, after plate
Formula filter, tripod pendulum type batch centrifugal use Bag filter, and board-like centrifuge is filtered using double-layer filter paper, record turning for tripod pendulum type batch centrifugal
The pressure parameter of speed and leaf filter, it is as a result as follows:
Embodiment | Filter situation | Tripod pendulum type batch centrifugal revolving speed | Leaf filter pressure |
Embodiment 1 | It is filtered quickly through tripod pendulum type batch centrifugal and leaf filter, tripod pendulum type batch centrifugal does not need to change cloth bag, and leaf filter does not need to change filter paper | 2000r/min | 0.2MP |
Embodiment 2 | It is filtered quickly through tripod pendulum type batch centrifugal and leaf filter, tripod pendulum type batch centrifugal does not need to change cloth bag, and leaf filter does not need to change filter paper | 2000r/min | 0.2MP |
Embodiment 3 | It is filtered quickly through tripod pendulum type batch centrifugal and leaf filter, tripod pendulum type batch centrifugal does not need to change cloth bag, and leaf filter does not need to change filter paper | 2000r/min | 0.2MP |
Comparative example 1 | More difficult logical fruit tripod pendulum type batch centrifugal and leaf filter, tripod pendulum type batch centrifugal change cloth bag 3 times, and leaf filter changes filter paper 6 times | 4000r/min | 0.6MP |
Comparative example 2 | More difficult logical fruit tripod pendulum type batch centrifugal and leaf filter, tripod pendulum type batch centrifugal change cloth bag 4 times, and leaf filter changes filter paper 6 times | 4000r/min | 0.6MP |
Comparative example 3 | More difficult logical fruit tripod pendulum type batch centrifugal and leaf filter do not refilter after tripod pendulum type batch centrifugal changes cloth bag 6 times, and gained filtrate is filtered using leaf filter, after changing filter paper 10 times, It is not filtering. | 4000r/min | 0.6MP |
Comparative example 4 | It is filtered by tripod pendulum type batch centrifugal and leaf filter, tripod pendulum type batch centrifugal changes cloth bag 2 times, and leaf filter changes filter paper 2 times. | 3000r/min | 0.4MP |
Remarks:Tripod pendulum type batch centrifugal maximum (top) speed is 4000r/min, and the pressure of leaf filter is greater than 0.6Mpa and booster easily occurs,
Therefore maximum control is in 0.6Mpa.
It analyzes from the above, enzymolysis liquid filters smoothly in the preparation method of snakehead enzymolysis liquid of the invention.
Nitrogen content detection
Enzymolysis liquid obtained by Example 1-3 and comparative example 1-4, according to《Chinese Pharmacopoeia 2015 editions four》In annex 0704
The first method detects in n2 method, and calculated result is as follows:
Embodiment | Detection level(%) | Filtrate gross weight(kg) | Total nitrogen content |
Embodiment 1 | 24.6 | 75.4 | 18.5484 |
Embodiment 2 | 33.4 | 53.9 | 18.0026 |
Embodiment 3 | 20.7 | 94.8 | 19.6236 |
Comparative example 1 | 19.5 | 66.2 | 12.909 |
Comparative example 2 | 23.5 | 67.9 | 15.9565 |
Comparative example 3 | 18.7 | 31.5 | 5.8905 |
Comparative example 4 | 20.5 | 72.9 | 14.9445 |
From above-mentioned test result analysis, the total nitrogen content of gained is significantly increased through the invention.
Claims (7)
1. a kind of preparation method of snakehead enzymolysis liquid, preparation step are as follows:
(1)Take fresh snakehead meat into enzymatic vessel, addition is equivalent to fresh 2-4 times of the snakehead meat water measured, and stirs, and lactic acid, heating is added
It is 40-60 DEG C to temperature, constant temperature is kept for 4-8 hours, and alkali regulator is added, and adjusting environmental system pH is 7-9;
(2)Protease is added, digests 3-5 hours, heating is boiled 5-15 minutes, is cooled to 20-40 DEG C, and lecithin, constant temperature is added
It is kept for 5-15 hours, filtering obtains enzymolysis liquid.
2. a kind of preparation method of snakehead enzymolysis liquid according to claim 1, it is characterised in that the lactic acid additional amount
For the 1-5% of fresh snakehead meat quality.
3. a kind of preparation method of snakehead enzymolysis liquid according to claim 2, it is characterised in that the lactic acid additional amount
It is the 3% of fresh snakehead meat quality.
4. a kind of preparation method of snakehead enzymolysis liquid according to claim 1, it is characterised in that the alkali regulator is
One of sodium hydroxide, potassium hydroxide, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium carbonate or several compositions.
5. a kind of preparation method of snakehead enzymolysis liquid according to claim 1, it is characterised in that the protease is added
Amount is the 1-5% of fresh snakehead meat quality.
6. a kind of preparation method of snakehead enzymolysis liquid according to claim 1, it is characterised in that the lecithin is added
Amount is the 0.1-0.7% of integral system quality.
7. a kind of preparation method of snakehead enzymolysis liquid according to claim 6, it is characterised in that the lecithin is added
Amount is the 0.4% of integral system quality.
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CN106962827A (en) * | 2017-04-18 | 2017-07-21 | 湖南文理学院 | A kind of protect liver snakeheaded fish nutrition oral formulations and its production method |
CN108420041A (en) * | 2018-03-19 | 2018-08-21 | 广州市凯虹香精香料有限公司 | A method of preparing natural cheeses essence |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN1101264A (en) * | 1993-12-22 | 1995-04-12 | 湖南省水产科学研究所 | Snakehead oral liquid and its process |
CN1344542A (en) * | 2001-09-12 | 2002-04-17 | 单佩南 | Production process and product of instant osteocalcium composition |
CN1476782A (en) * | 2002-08-20 | 2004-02-25 | 北京京港耀新生物技术开发有限公司 | Preparation process of complete-component pearl product |
CN101455438A (en) * | 2009-01-04 | 2009-06-17 | 湖北友芝友生物科技有限公司 | Freeze-drying production method of nutrient material |
CN101816367A (en) * | 2010-04-12 | 2010-09-01 | 田颖刚 | Method for preparing mullet active peptide |
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CN106267054A (en) * | 2015-06-04 | 2017-01-04 | 湖南明瑞制药有限公司 | Compound recipe Ophicephalus argus capsule and preparation method thereof |
CN105779098A (en) * | 2016-04-06 | 2016-07-20 | 常州市鼎日环保科技有限公司 | Method for refining fish oil from leftovers of freshwater fish |
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CN108420041A (en) * | 2018-03-19 | 2018-08-21 | 广州市凯虹香精香料有限公司 | A method of preparing natural cheeses essence |
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