CN108902478A - A kind of composite feed additive and its application for improving Pelteobagrus fulvidraco immunity - Google Patents
A kind of composite feed additive and its application for improving Pelteobagrus fulvidraco immunity Download PDFInfo
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- CN108902478A CN108902478A CN201810606581.1A CN201810606581A CN108902478A CN 108902478 A CN108902478 A CN 108902478A CN 201810606581 A CN201810606581 A CN 201810606581A CN 108902478 A CN108902478 A CN 108902478A
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- 241000376029 Tachysurus fulvidraco Species 0.000 title claims abstract description 49
- 239000003674 animal food additive Substances 0.000 title claims abstract description 32
- 239000002131 composite material Substances 0.000 title claims abstract description 30
- 230000036039 immunity Effects 0.000 title claims abstract description 29
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 claims abstract description 46
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 36
- 239000000654 additive Substances 0.000 claims abstract description 26
- 229940109262 curcumin Drugs 0.000 claims abstract description 23
- 235000012754 curcumin Nutrition 0.000 claims abstract description 23
- 239000004148 curcumin Substances 0.000 claims abstract description 23
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000011716 vitamin B2 Substances 0.000 claims abstract description 23
- 229920001661 Chitosan Polymers 0.000 claims abstract description 21
- 230000000996 additive effect Effects 0.000 claims abstract description 21
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 18
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- 239000011718 vitamin C Substances 0.000 claims abstract description 18
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- CIWBSHSKHKDKBQ-UHFFFAOYSA-N 2-(1,2-dihydroxyethyl)-3,4-dihydroxy-2h-furan-5-one Chemical compound OCC(O)C1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-UHFFFAOYSA-N 0.000 description 1
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- SXZYCXMUPBBULW-SKNVOMKLSA-N L-gulono-1,4-lactone Chemical compound OC[C@H](O)[C@H]1OC(=O)[C@@H](O)[C@H]1O SXZYCXMUPBBULW-SKNVOMKLSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
A kind of composite feed additive and its application for improving Pelteobagrus fulvidraco immunity, it is characterised in that the additive is by curcumin, chitosan, vitamin C, vitamin B2It is formed at least one auxiliary material suitable for feed addictive, the curcumin, chitosan, vitamin C and vitamin B2Weight ratio be 100~200：4000~5000：659~759:20~60.The proportion of each component and Pelteobagrus fulvidraco basal feed is in the composite feed additive：The additive capacity of curcumin is 100~200mg/kg basal feed, and the additive capacity of chitosan is 4000~5000mg/kg basal feed, and the ascorbic additive capacity is 659~759mg/kg basal feed, vitamin B2Additive capacity is 20~60mg/kg basal feed.For preventing Pelteobagrus fulvidraco infection Aeromonas hydrophila.
Description
Technical field
The present invention relates to technical field of aquaculture, further to the immunological regulation technology for being directed to fish, and in particular to
A kind of composite feed additive and its application for improving Pelteobagrus fulvidraco immunity.
Background technique
Pelteobagrus fulvidraco (Pelteobagrus fulvidraco) is commonly called as yellow aunt, yellow wax, loud, high-pitched sound fish, loud, high-pitched sound serratd edge etc., belongs to Nian shape
Mesh, Chang section, Pelteobagrus are a kind of small-sized economic freshwater fish, the major water system in China are distributed in, especially in the middle and lower reach of Yangtze River
Lake, pond are widely present in streams.Pelteobagrus fulvidraco protein content is high, and fat content is few, full of nutrition, fine and tender taste, economical
Value is higher, and has tonic effect and medical value, quite by consumer's pro-gaze.Not only domestic market demand amount was gradually in recent years
Increase, or even be exported to Japan, South Korea, Southeast Asian countries etc., it is the excellent variety of foreign exchange earning, cultivation scale also increases year by year
Greatly, but with the increasing of cultivation scale, cultivation density, easily lead to anti-stress ability and the premunition decline of Pelteobagrus fulvidraco, vulnerable to
Disease invasion, result in significant economic losses.
Curcumin (curcumin) is a kind of relative molecular mass extracted from zingiberaceous plant Curcuma longa L.
Small polyphenols, have anti-inflammatory, anti-oxidant, scavenging activated oxygen, anti-peroxidation lipid mechanism harm, anti-aging, anti-fibrosis,
Protect a variety of physiological activity such as liver and kidney.Show that the curcumin of optimal dose can in the application study of aquaculture field
To improve the immunocompetence of fish.
Chitosan also known as chitin sugar, Chitosan, research shows that it is with preferable bacteriostatic activity, it can biology
Degradation, metabolite is nontoxic, can activated lymphocyte, improve immunity, adjust fat metabolism and reduce blood lipid, cholesterol
Etc. different physiological roles.Chitosan plays in extensive application as aquatic animal feed additive and improves survival rate, growth-promoting
Effect that is long and promoting fish body non-specific immunity.
The physiological action of vitamin C in animal body is extremely wide.It and hormone sensitive lipase gene, mineral absorption, metabolism
It is closely related, and to enhancing immunity of organisms, prevention scurvy, promote wound healing and crust growth and protein metabolism
In play an important role.But most of aquatic animal is due to lacking (the L-gulonolactone oxidation of Gu Luonei ester oxidase
Enzyme) and ascorbic acid usp/bp cannot be synthesized, this is the principal element for causing hypovitaminosis C in aquatic animal body, in bait
Middle addition vitamin C is grown to aquatic animal and immunology plays a significant role, and is mainly reflected in and improves immunity, improves life
Long performance, enhancing resistance to compression regulating power and oxidation resistance etc..
Vitamin B2Also known as riboflavin is one of water soluble vitamin necessary to animal, and it is anti-to participate in the reduction of body internal oxidition
Answer, cell metabolism and breathing chain reaction.Vitamin B in fish feed raw material2Level is not able to satisfy the needs of fish growth,
A certain amount of vitamin B2 must additionally be added to be supplemented, absolutely prove vitamin B2To the necessity of fish growth.
Some researches show that certain immunopotentiators of single addition, to intensity, the maintenance immunity time for improving immunity
Deng being limited, but the application report only cultivated on a small quantity about Chinese herbal formula in Pelteobagrus fulvidraco at present, and for how
Filter out the compound formula still shortage technical support for effectively adjusting Pelteobagrus fulvidraco immunity.
Summary of the invention
The present invention is directed to be directed to the technological deficiency of the prior art, provide a kind of for improving the compound feeding of Pelteobagrus fulvidraco immunity
Feed additives lack a kind of efficient Immune enhancement hand for Pelteobagrus fulvidraco to solve the prior art in Pelteobagrus fulvidraco breeding process
The technical issues of section.
Another technical problem to be solved by the present invention is that if conventional Pelteobagrus fulvidraco anti-pathogenic drug long-time service may produce
Raw drug resistance.
The invention solves another technical problem be that conventional Pelteobagrus fulvidraco anti-pathogenic drug may be in Pelteobagrus fulvidraco body
Enrichment, and then influence the edible health of the mankind.
The invention solves another technical problem be that single immunopotentiator function and effect are limited.
To realize that the above technical purpose, the present invention use following technical scheme：
It is a kind of for improving the composite feed additive of Pelteobagrus fulvidraco immunity, it is characterised in that the additive is by turmeric
Element, chitosan, vitamin C, vitamin B2It is formed at least one auxiliary material suitable for feed addictive, the curcumin, shell
Glycan, vitamin C and vitamin B2Weight ratio be 100~200：4000~5000：659~759:20~60.
Described is a kind of for improving the composite feed additive of Pelteobagrus fulvidraco immunity, it is characterised in that the curcumin,
Chitosan, vitamin C and vitamin B2Weight ratio be 140~160：4300~4700：644~674：35~45.
Described is a kind of for improving the composite feed additive of Pelteobagrus fulvidraco immunity, it is characterised in that described to be suitable for raising
The auxiliary material of feed additives is water.
It is described a kind of for improving the preparation method of the composite feed additive of Pelteobagrus fulvidraco immunity, include the following steps：
(1) by curcumin, chitosan, vitamin C, vitamin B2It is soluble in water respectively, corresponding aqueous solution is made；
(2) by curcumin, chitosan, vitamin C, vitamin B2Aqueous solution be uniformly mixed that obtain mixed solution be multiple
Close feed addictive.
The present invention also provides described a kind of for improving the application of the composite feed additive of Pelteobagrus fulvidraco immunity, spies
Sign is that the application mode is to mix composite feed additive with Pelteobagrus fulvidraco basal feed, in the composite feed additive
The proportion of each component and Pelteobagrus fulvidraco basal feed is：The additive capacity of curcumin is 100~200mg/kg basal feed, chitosan
Additive capacity be 4000~5000mg/kg basal feed, the ascorbic additive capacity be 659~759mg/kg basis
Feed, vitamin B2Additive capacity is 20~60mg/kg basal feed.
It is described a kind of for improving the application of the composite feed additive of Pelteobagrus fulvidraco immunity, it is characterised in that will be described multiple
It closes feed addictive and is uniformly sprayed on Pelteobagrus fulvidraco basal feed surface.
It is described a kind of for improving the application of the composite feed additive of Pelteobagrus fulvidraco immunity, it is characterised in that described compound
Feed addictive is for preventing Pelteobagrus fulvidraco infection Aeromonas hydrophila.
The composite feed additive and its application, the present invention that the present invention provides a kind of for improving Pelteobagrus fulvidraco immunity mention
The multiple feed addictive supplied is sent out by the proportion of four kinds of ingredients of optimization and to the adding proportion of basal feed by zoopery
It is existing, with curcumin additive capacity be 100~200mg/kg basal feed, chitosan additive capacity be 4000~5000mg/kg
Basal feed, ascorbic additive capacity are 659~759mg/kg basal feed, vitamin B2Additive capacity is 20~60mg/
Kg basal feed feeds Pelteobagrus fulvidraco, its catalase in serum (CAT) vigor, Serum glutathione (GSH) after 60 days
Content, serum alkaline phosphatase (AKP) vigor, serum lysozyme (LZM) vigor, seralbumin (ALB) content, one oxygen of serum
Change nitrogen (NO) content to significantly improve, Serum MDA (MDA) content significantly reduces, and it is yellow to significantly reduce infection Aeromonas hydrophila
The cumulative mortality of forehead fish.Based on the above experimental result, it is determined that with the composite feed additive of technical solution offer and its answer
With, can adjust Pelteobagrus fulvidraco non-specific immunity on the basis of effectively promote its disease-resistant performance.The present invention is based on rigorous
Laboratory facilities obtain beneficial technical effect, composite immune reinforcing agent in the present invention, and each raw material sources are stablized, can effectively be kept away
Exempt from same immunizing agent is used for a long time and develop drug resistance, under field conditions (factors) can be degradable, while cost is relatively low, it is real to be easy to
It is existing, therefore there is good promotion prospect.Furthermore it was also found that preferred four kinds of component proportions and to feeding in option screening
Additive amount in material may be implemented preferably to enhance immune effect.
Specific embodiment
Below by specific embodiments of the present invention will be described in detail.In order to avoid excessive unnecessary details,
It will not be described in detail in following embodiment to belonging to well known structure or function.In addition to being defined, institute in following embodiment
Technical and scientific term has the identical meanings being commonly understood by with those skilled in the art of the invention.
It is provided by the invention a kind of for improving the composite feed additive of Pelteobagrus fulvidraco immunity, it is made of following raw material：
Curcumin, chitosan, vitamin C, vitamin B2.The preparation method of the composite feed additive, includes the following steps：
(1) by curcumin, chitosan, vitamin C, vitamin B2It is soluble in water respectively, corresponding aqueous solution is made；
(2) by curcumin, chitosan, vitamin C, vitamin B2Aqueous solution be uniformly mixed that obtain mixed solution be multiple
Close feed addictive.
Curcumin additive capacity is 100~200mg/kg basal feed, and the additive capacity of chitosan is 4000~5000mg/
Kg basal feed, ascorbic additive capacity are 659~759mg/kg basal feed, vitamin B2Additive capacity be 20~
60mg/kg basal feed.
Basal feed is Pelteobagrus fulvidraco buoyancy expanding feed in present embodiment, is purchased from the limited public affairs of Tianjin morning brightness feed
Department, the nutrient level of basal feed are as follows
Crude protein >=36.0%, crude fat >=6.0%, crude fibre≤5.0%, coarse ash≤15%, calcium 0.5%~
2.5%, total phosphorus 0.6%~2.0%, sodium chloride 0.3%~3.0%, lysine >=1.7%, moisture≤12.0%.
In embodiment each group distribution ratio and application when such as following table (unit：Parts by weight)
| Embodiment number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| Curcumin | 100 | 150 | 200 | 140 | 160 | 140 | 160 | 100 | 200 |
| Chitosan | 4000 | 4500 | 5000 | 4300 | 4700 | 4700 | 4300 | 5000 | 4000 |
| Vitamin C | 659 | 709 | 759 | 694 | 724 | 694 | 724 | 659 | 759 |
| Vitamin B | 20 | 40 | 60 | 35 | 45 | 45 | 35 | 60 | 20 |
The application mode of the composite feed additive is that the mixed solution as compound additive is packed into micro- mist watering can,
Uniformly it is sprayed on Pelteobagrus fulvidraco basal feed surface.
Test example
This test is carried out in Tianjin Landtek aquatic products Co., Ltd, using Pelteobagrus fulvidraco as subjects, selects neat specification, body
The male Pelteobagrus fulvidraco of lattice stalwartness, 56.67 ± 10.75g of original body mass, the long 15.86 ± 1.23cm of first initial body, random point is supported in 21
In box for breeding, every 50 tail of case temporarily supports 10 days before formal test, feeds basal feed.Test is divided into 7 groups, every group of three repetitions.
Other cultivating conditions are suitable and identical, and different tests group additive addition situation see the table below
The mode of feeding is：It feeds sooner or later daily 2 times, all using the method for breeding fed of being satiated with food.
After cultivating 8 weeks, salivary lysozyme measurement and challenge viral dosage are carried out to each test group.
The acquisition of salivary lysozyme sample, each test group randomly select 10 tail fishes, carry out tail vein blood.It is taken a blood sample
Routinely method collects serum to liquid sample, for the measurement of related salivary lysozyme, as a result such as following table
Challenge viral dosage
Use Aeromonas hydrophila (Aeromonas hydrophila, A.h), after bacterium activation, sterile PBS buffer
Degree of thickening is to 2 × 108Cells/mL, the Pelteobagrus fulvidraco long to body, weight is almost the same carry out attacking malicious injection, and every case takes 10 tail fishes,
Every group of 30 tail of processing, and by the dosage of every 100g fish intraperitoneal injection bacterium solution 2mL, keep cultivating condition constant, timing is observed daily
And fish body death condition is recorded, the test period is 1 week, and the cumulative mortality of statistics each group fish see the table below (average value)
The embodiments of the present invention have been described in detail above, but the content is only section Example of the invention,
It is not intended to limit the invention.All any modifications, equivalent replacements, and improvements etc. done in application range of the invention, should all
It is included within protection scope of the present invention.
Claims (7)
1. a kind of for improving the composite feed additive of Pelteobagrus fulvidraco immunity, it is characterised in that the additive by curcumin,
Chitosan, vitamin C, vitamin B2It is formed at least one auxiliary material suitable for feed addictive, the curcumin, shell are poly-
Sugar, vitamin C and vitamin B2Weight ratio be 100~200：4000~5000：659~759:20~60.
2. as described in claim 1 a kind of for improving the composite feed additive of Pelteobagrus fulvidraco immunity, it is characterised in that institute
State curcumin, chitosan, vitamin C and vitamin B2Weight ratio be 140~160：4300~4700：644~674：35~
45。
3. as claimed in claim 1 or 2 a kind of for improving the composite feed additive of Pelteobagrus fulvidraco immunity, it is characterised in that
The auxiliary material suitable for feed addictive is water.
4. a kind of for improving the preparation side of the composite feed additive of Pelteobagrus fulvidraco immunity as described in claims 1 to 3 is any
Method includes the following steps：
(1) by curcumin, chitosan, vitamin C, vitamin B2It is soluble in water respectively, corresponding aqueous solution is made；
(2) by curcumin, chitosan, vitamin C, vitamin B2Aqueous solution be uniformly mixed that obtain mixed solution be compound feeding
Feed additives.
5. it is a kind of for improving the application of the composite feed additive of Pelteobagrus fulvidraco immunity as described in claims 1 to 3 is any,
It is characterized in that the application mode is to mix composite feed additive with Pelteobagrus fulvidraco basal feed, the mixed feed addition
The proportion of each component and Pelteobagrus fulvidraco basal feed is in agent：The additive capacity of curcumin is 100~200mg/kg basal feed, shell
The additive capacity of glycan is 4000~5000mg/kg basal feed, and the ascorbic additive capacity is 659~759mg/kg
Basal feed, vitamin B2Additive capacity is 20~60mg/kg basal feed.
6. a kind of for improving the application of the composite feed additive of Pelteobagrus fulvidraco immunity as described in claim 54, feature exists
In the composite feed additive is uniformly sprayed on Pelteobagrus fulvidraco basal feed surface.
7. a kind of for improving the application of the composite feed additive of Pelteobagrus fulvidraco immunity, feature as described in claim 5 or 6
It is the composite feed additive for preventing Pelteobagrus fulvidraco infection Aeromonas hydrophila.
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