CN108893277A - One kind splitting formula leakproof visible surface sampling culture dish out - Google Patents
One kind splitting formula leakproof visible surface sampling culture dish out Download PDFInfo
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- CN108893277A CN108893277A CN201810894844.3A CN201810894844A CN108893277A CN 108893277 A CN108893277 A CN 108893277A CN 201810894844 A CN201810894844 A CN 201810894844A CN 108893277 A CN108893277 A CN 108893277A
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- wall
- ware
- downside wall
- ware downside
- culture dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses one kind to split formula leakproof visible surface sampling culture dish out, the culture dish includes ware bottom and ware lid, it can split set on the transversal crack mouth of the first ware downside wall and longitudinal crack mouth when stressed, it is detached from movable ware downside wall, the height of the first ware downside wall is caused to be lower than the plane of solid medium, therefore the operation of surface microorganism sampling is easier.In addition, the second ware downside wall can prevent culture medium from overflowing from ware bottom, observation window can make the observation of minute colony more convenient.
Description
Technical field
The present invention relates to a kind of experiment equipment, especially one kind to split formula leakproof visible surface sampling culture dish out.
Background technique
Culture dish is one of most widely used experiment equipment in biology, is usually used in the objects such as packaging material for food, tableware
The culture of body surface microorganism is tested, and the sampling of body surface microorganism usually wipes determinand body surface with sterile cotton swab
Cotton swab or filter paper are perhaps transferred in buffer with aseptic filter paper abutting object under test surface later and are eluted, to reach by face
To the purpose of sampling.But cotton swab or filter paper can not discharge microorganism collected completely, be easy to cause Bacterial diversity
Incomplete culture;In addition the sampling of body surface microorganism needs sterile working, and cotton swab or filter paper are carrying out microorganism
Acquisition and elution during, be easy pollution by environmental microorganism, be extremely difficult to the requirement of sterile working;These disadvantages
It will cause the deviation of body surface microorganism testing result.In addition, the operating process of the above method is cumbersome, inefficiency, hold
Easily cause the waste of human resources.When the bacterium colony for the target culture that the culture medium in culture dish is grown is smaller, need to use
Microscope by bacterium colony amplify by observe bacterium colony it is accurate in the form of, need to open in culture dish during magnifying bacterium colony,
This may bring pollution or the leakage of harmful culture.
Summary of the invention
For disadvantages mentioned above, the purpose of the present invention is to provide a kind of splitting out of being simple and convenient to operate of structure, formula leakproof can
Apparent surface sampling culture dish.The implementation of the culture dish can simplify the sampling process of body surface microorganism, improve test result
Accuracy, improve working efficiency.
For splitting formula leakproof visible surface sampling culture dish out, to reach the purpose, the technical solution adopted by the present invention is that:
One kind splitting formula leakproof visible surface sampling culture dish out, the ware lid being covered on ware bottom including ware bottom and relaxation, it is characterised in that:
The ware lid include tegmental wall and at least one from tegmental wall around the ware lid side wall holded up, the ware bottom includes bottom wall and at least
One from bottom wall around the first ware downside wall holded up;The first ware downside wall is equipped at least one circle transversal crack mouth, institute
The transversal crack choma stated around described first ware downside wall one week, the circle plane of the transversal crack mouth and the bottom wall it is flat
Face is parallel;The first ware downside wall is divided into movable ware downside wall and fixed ware downside wall by the transversal crack mouth;
The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack wall, the transversal crack wall
Thickness is less than the first ware downside wall;The movable ware downside wall be equipped at least one perpendicular to the transversal crack mouth longitudinal direction
The movable ware downside wall of crackle mouth, longitudinal crack mouth two sides is connected by longitudinal crack wall, the thickness of the longitudinal crack wall
Degree is less than the first ware downside wall;The diameter of the ware lid side wall is greater than the first ware downside wall;The diameter of the bottom wall is greater than
The tegmental wall;The height of the activity ware downside wall and the sum of the height of the fixed ware downside wall, are less than the ware lid side wall
Height;The culture dish further includes that at least one surround the second ware downside wall holded up from bottom wall, the second ware downside wall
Height is less than the ware lid side wall, and the diameter of the second ware downside wall is greater than ware lid side wall, the first ware downside wall and the second ware bottom
Side wall is located at the same side of the bottom wall;It is additionally provided at least one transparent observation window in the tegmental wall, the observation window is in
Convex lens shape, and thickeied by the tegmental wall center and formed.
Compared with the prior art, the beneficial effects of the invention are as follows:The longitudinal crack wall and transversal crack wall of the culture dish
Thickness very little, be easy to rupture under external force, when applying force to the movable ware downside wall, the first ware bottom can be made
Side wall splits at longitudinal crack mouth and transversal crack mouth, with removal activity ware downside wall, and then makes the height of the first ware downside wall
Degree decreases below the plane of solid medium, and the solid culture base plane is directly covered in determinand body surface when use
Face can reach the purpose of sampling microorganisms from surface of object.Therefore the structure of the culture dish is simple, easy to use, Ke Yijian
The process for changing sampling microorganisms from surface of object overcomes the problems, such as to be easy to be polluted by environmental microorganism in sampling process, improve simultaneously
Test result accuracy and working efficiency.In addition, the second ware downside wall can prevent culture medium from overflowing from ware bottom, observation window can make micro-
The observation of petite is more convenient.
Detailed description of the invention
Illustrate technical solution of the present invention in order to clearer, it below will be to attached drawing needed in embodiment description
Make brief introduction, it should be apparent that, drawings in the following description are only some embodiments of the invention, general for this field
For logical technical staff, without creative efforts, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structural schematic diagram for splitting formula leakproof visible surface sampling culture dish out;Fig. 2 is the movable ware downside wall
Cross-sectional view;Fig. 3 is the partial enlargement diagram of the first ware downside wall;Respectively number is respectively in figure:
1 is ware lid, and 2 be ware bottom, and 3 be tegmental wall, and 4 be bottom wall, and 5 be ware lid side wall, and 6 be the second ware downside wall, and 7 be the first ware bottom side
Wall, 71 be movable ware downside wall, and 72 be transversal crack mouth, and 73 be transversal crack wall, and 74 be longitudinal crack mouth, and 75 be longitudinal crack
Wall, 76 be fixed ware downside wall, and 13 be gap, and 21 be observation window.
Specific embodiment
It is right below in conjunction with the drawings and specific embodiments in order to be more clear the objectives, technical solutions, and advantages of the present invention
The present invention is described in further detail.It should be appreciated that described embodiment is only used to explain the present invention, it is all to be based on
Simplified or equivalent variations, all belongs to the scope of protection of the present invention made by technical solution of the present invention.
In the discussion of specific embodiment, the shape for splitting formula leakproof visible surface sampling culture dish out is preferably selected
Circle, still, those skilled in the art should be understood that it is suitable for such as ellipse, rectangle or other suitable
Shape for culture dish.
The invention discloses one kind to split formula leakproof visible surface sampling culture dish out, including ware bottom 2 and relaxation are covered in ware
Ware lid 1 on bottom 2, the ware lid 1 includes tegmental wall 3 and at least one surround the ware lid side wall 5 holded up from tegmental wall 3, described
Ware bottom 2 includes bottom wall 4 and at least one surround the first ware downside wall 7 holded up from bottom wall 4;The first ware downside wall 7 is set
There is at least one circle transversal crack mouth 72, the transversal crack mouth 72 around described first ware downside wall 7 one weeks, split by the transverse direction
The circle plane of line mouth 72 is parallel with the plane of the bottom wall 4;The first ware downside wall 7 is by the transversal crack mouth 72
It is divided into movable ware downside wall 71 and fixed ware downside wall 76;The movable ware downside wall 71 and the fixation ware downside wall
76 coupling part is transversal crack wall 73, and the thickness of the transversal crack wall 73 is less than the first ware downside wall 7;The activity
Ware downside wall 71 be equipped at least one perpendicular to the transversal crack mouth 72 longitudinal crack mouth 74,74 liang of the longitudinal crack mouth
The movable ware downside wall 71 of side is connected by longitudinal crack wall 75, and the thickness of the longitudinal crack wall 75 is less than the first ware downside wall
7.Features described above has determined the basic structure for splitting formula leakproof visible surface sampling culture dish out, wherein 75 He of longitudinal crack wall
The thickness very little of transversal crack wall 73 is easy to rupture under external force, makes the first ware downside wall 7 in longitudinal crack mouth 74 and cross
It splits to crackle mouth 72, movable ware downside wall 71 can be detached from from fixed ware downside wall 76 at this time.And the first ware downside wall 7
The space formed with bottom wall 4 is as culture inner cavity, when cultivating inner cavity by the enough agar mediums of injection(The culture medium
Liquid level is higher than the transversal crack mouth 72), after the culture medium solidification, apply force to the movable ware downside wall
71, so that the first ware downside wall 7 is split at longitudinal crack mouth 74 and transversal crack mouth 72, with removal activity ware downside wall 71, into
And the height of fixed ware downside wall 76 is made to decrease below the plane of the agar medium, by the agar culture when use
Base plane is directly covered in object under test surface, that is, can reach the purpose of sampling microorganisms from surface of object, avoid using cotton swab
Son or filter paper carry out the tedious steps eluted again after microorganism acquisition, greatly simplify the mistake of sampling microorganisms from surface of object
Journey, and overcome and be easy the problem of being polluted by environmental microorganism in sampling process, at the same improve test result accuracy and
Working efficiency.
It is split in the embodiment of formula leakproof visible surface sampling culture dish out described, the diameter of the bottom wall 4 is greater than described
The diameter of tegmental wall 3, the setting of this feature can be limited when ware lid 1 and the fastening of ware bottom 2, and ware lid side wall 5 can only be supported in bottom wall
4.In addition, the height of the activity ware downside wall 71 and the sum of the height of the fixed ware downside wall 76, are less than the ware lid side
The height of wall 5, this feature can be limited to be existed centainly between activity ware downside wall 71 and tegmental wall 3 when ware lid 1 and the fastening of ware bottom 2
Gap, prevent the agar medium from contacting with tegmental wall 3.
The culture dish further includes that at least one surround the second ware downside wall 6 holded up, the first ware downside wall from bottom wall 4
7 and second ware downside wall 6 be located at the same side of the bottom wall 4.The diameter of the ware lid side wall 5 is greater than the first ware downside wall 7
Diameter, but less than the diameter of the second ware downside wall 6, this feature is limited when the ware lid 1 and the ware bottom 2 fastening, ware lid
Side wall 5 is fastened on the centre of the first ware downside wall 7 and the second ware downside wall 6 just.In addition, the height of the second ware downside wall 6 is small
In the ware lid side wall 5, this feature can prevent the second ware downside wall 6 from hindering the unlatching of the ware lid 1 with ware bottom 2
Or it fastens.First ware downside wall 7 and the second ware downside wall 6 and bottom wall 4 form gap 13, the gap 13 can collect to
The agar medium of spill and leakage, prevents the culture medium from overflowing from ware bottom 2 when injecting agar medium in culture dish.
The tegmental wall 3 is additionally provided at least one transparent observation window 21, and the observation window 21 is in convex lens shape, and by
The center of the tegmental wall 3 thickeies and is formed, therefore the observation window 21 can be made micro- with the bacterium colony of amplification target culture
The observation of petite is more convenient, and can prevent the possible dirt because opening ware lid 1 and ware bottom 2 during observation bacterium colony
The leakage of dye or harmful culture.
Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute
The embodiment of description is only a part of the embodiments of the present invention, and non-limiting exhaustion.Based on the embodiments of the present invention, originally
Field those of ordinary skill all other embodiment obtained under the premise of not making the creative labor, belongs to this hair
Bright protection scope.
Claims (1)
1. one kind splits formula leakproof visible surface sampling culture dish out, special including the ware lid that ware bottom and relaxation are covered on ware bottom
Sign is:The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up from tegmental wall, and the ware bottom includes bottom
Wall is at least one from bottom wall around the first ware downside wall holded up;The first ware downside wall is equipped at least one circle and laterally splits
Line mouth, the transversal crack choma around described first ware downside wall one week, the circle plane of the transversal crack mouth with it is described
The plane of bottom wall is parallel;The first ware downside wall is divided into movable ware downside wall and fixed ware by the transversal crack mouth
Downside wall;The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack wall, the transverse direction
The thickness of crackle wall is less than the first ware downside wall;The movable ware downside wall is equipped at least one perpendicular to the transversal crack
The longitudinal crack mouth of mouth, the movable ware downside wall of longitudinal crack mouth two sides are connected by longitudinal crack wall, and the longitudinal direction is split
The thickness of line wall is less than the first ware downside wall;The diameter of the ware lid side wall is greater than the first ware downside wall;The bottom wall
Diameter is greater than the tegmental wall;The height of the activity ware downside wall and the sum of the height of the fixed ware downside wall, are less than described
The height of ware lid side wall;The culture dish further includes that at least one surround the second ware downside wall holded up, the second ware from bottom wall
The height of downside wall is less than the ware lid side wall, and the diameter of the second ware downside wall is greater than ware lid side wall, the first ware downside wall and
Second ware downside wall is located at the same side of the bottom wall;It is additionally provided at least one transparent observation window in the tegmental wall, it is described
Observation window is in convex lens shape, and is thickeied by the tegmental wall center and formed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810894844.3A CN108893277A (en) | 2018-08-08 | 2018-08-08 | One kind splitting formula leakproof visible surface sampling culture dish out |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810894844.3A CN108893277A (en) | 2018-08-08 | 2018-08-08 | One kind splitting formula leakproof visible surface sampling culture dish out |
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Publication Number | Publication Date |
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CN108893277A true CN108893277A (en) | 2018-11-27 |
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CN201810894844.3A Withdrawn CN108893277A (en) | 2018-08-08 | 2018-08-08 | One kind splitting formula leakproof visible surface sampling culture dish out |
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CN (1) | CN108893277A (en) |
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2018
- 2018-08-08 CN CN201810894844.3A patent/CN108893277A/en not_active Withdrawn
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Application publication date: 20181127 |