CN108887110A - The implantation methods of Gynura divaricata - Google Patents
The implantation methods of Gynura divaricata Download PDFInfo
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- CN108887110A CN108887110A CN201810878889.1A CN201810878889A CN108887110A CN 108887110 A CN108887110 A CN 108887110A CN 201810878889 A CN201810878889 A CN 201810878889A CN 108887110 A CN108887110 A CN 108887110A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- Chemical & Material Sciences (AREA)
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Abstract
The present invention relates to the implantation methods of Chinese herbal medicine, and in particular to is a kind of implantation methods of Gynura divaricata.The technical solution used for：The implantation methods of Gynura divaricata, include the following steps：1) the young sprout Gynura divaricata stem section for taking current year raw prepares stem with bud, is immersed in spare in fulvic acid solution；2) Gynura divaricata extracting solution is prepared；3) stubble for preparing Gynura divaricata extracting solution and filter residue are mixed, mixture and peat, vermiculite, garden mould press 1:2:1:After 1 ratio mixing, by the water content for being used to adjust mixture after the dilution of Gynura divaricata extracting solution to 60-80%, sterilizes, obtain culture substrate；4) whole day illumination will be kept in the culture substrate of stem with bud embedment step 3), in seedling rearing room, and carry out shading treatment with single layer shading net；5) water content for keeping culture substrate is 50-60%；Completion nursery in 15-20 days, obtains basic seedling；The root system for being bred as seedling can rapidly adapt to field soil environment, and survival rate significantly improves.
Description
Technical field
The present invention relates to the implantation methods of Chinese herbal medicine, and in particular to is a kind of implantation methods of Gynura divaricata.
Background technique
Gynura divaricata also known as Bai Dongfeng, white chessman grass, beautiful loquat, asiatic toddalia root-bark, thick musculus cutaneus, chicken dish, big fat cow, white kind of amaranth, Bai Hong
Dish, rich and honour dish, Radix et Rhizoma Gynurae divaricatae etc. are distributed in TaiWan, China to south China, a southwestern band.Its root, cauline leaf can be used as medicine；Its root nature and flavor
It is sweet, cool, energy clearing heat and cooling blood, eliminating stasis to subdue swelling；Its stem, is trembled with fear at the salty micro-pungent of leaf taste, toxic, has heat-clearing, relaxing muscles and tendons, hemostasis, the function of eliminating the phlegm
Effect.Containing nutrients such as a large amount of phycocolloid element mannitol vitamin B phosphorus potassium and a variety of amino acid in its cauline leaf, thus there is pole
Strong reducing blood pressure and blood fat inhibits the effect of diabetes.Indoor placement can also play the effect of sterilizing and purifying air.20th century 90
Later period in age is gradually artificial cultivation from naturally wild development, integrates medicinal, edible, ornamental value, have wide exploitation
Prospect.
Radix et Rhizoma Gynurae divaricatae robustness is poor, and seed amount is few, and seedling is slow, and frequently with cutting propagation in production, root induction is to determine
Determine the key technique that can cuttage survive, and auxin type and concentration and nutrition content are to influence water culture skewer
An important factor for inserting rooting rate, the amount of taking root, root system and plant growth condition.Existing method is mostly with Radix et Rhizoma Gynurae divaricatae stem section or leaf
Piece generates callus, and neomorph then occurs and forms plant, finally takes root for forming intact plant；Or it is trained with the induction of the lateral bud
It supports and forms plant, but breeding coefficient is lower；And mostly suspension culture, the root system born will pass through longer time adapted soil
Environment, survival rate is low after transplanting.
Summary of the invention
The purpose of invention is to provide a kind of Gynura divaricata implantation methods of high survival rate.To achieve the above object, the present invention mentions
For following technical solution：
The implantation methods of Gynura divaricata, include the following steps：
1) the young sprout Gynura divaricata stem section that current year is raw is taken, blade is cut off and retains petiole, after irrigation and disinfection, is cut into 0.5-1.0cm
Stem with bud, be immersed in concentration be 0.5-1% fulvic acid solution in it is spare；
2) the Gynura divaricata plant that will obtain young sprout stem section scrapes epidermis after removing blade, after being crushed together with root,
Broken wall treatment, the raw material after broken wall are centrifugated after being mixed with 3-5 times of clear water, obtain a clear liquid, filter residue adds 2-3
Times clear water is centrifugated after being mixed, and obtains secondary clear liquid, clear liquid is mixed to get Gynura divaricata extracting solution twice；
3) it after step 2) being removed blade, epidermis and the crushing of the Gynura divaricata stubble of root, is centrifugated twice with step 2)
Filter residue mixing, mixture and peat, vermiculite, garden mould press 1:2:1:After 1 ratio mixing, by the Gynura divaricata extracting solution of step 2)
Water content after 5-10 times of dilution for adjusting mixture carries out sterilization treatment, obtains culture substrate to 60-80%；
4) stem with bud of step 1) is taken out, is embedded in the culture substrate of step 3), exposed portion is less than the 1/ of overall length
5, seedling rearing room is interior to keep whole day illumination, and carries out shading treatment with single layer shading net；
5) it when the water content of culture substrate is lower than 30%, is poured with 5-10 times of diluted Gynura divaricata extracting solution,
The water content for keeping culture substrate is 50-60%；Completion nursery in 15-20 days, obtains basic seedling.
By basic transplantation of seedlings to crop field, planting density is 2000-2500 plants/acre, sprinkles profoundly water once, is hidden with the double-deck shading net
Gear processing 3-5 days, then change single layer shading net into and block processing 5-7 days, ground moistening, shading treatment are kept during shading treatment
After can carry out normal water and fertilizer management.
Particularly, step 4), 5) be hole plate seedling growth, obtained basic seedling transplants together with culture substrate.
The present invention has the advantages that：
1, stem with bud is impregnated with fulvic acid solution, on the one hand can during preparing extracting solution and culture substrate
The activity of stem with bud is kept, meanwhile, when taking out stem with bud, remain in the Huang of stem with bud surface, especially wound surface
Rotten acid can play the role of anti-corrosion and certain growth-promoting root.
2, take root nutrient solution and water for irrigation are replaced using Gynura divaricata extracting solution, and uses round-the-clock shading type illumination
Condition can promote stem with bud to take root, so that seedling raise period shortens 3-5 days than existing ciltivating process.Meanwhile using Gynura divaricata
Extracting solution cost is also more much lower than preparing nutrient solution of taking root.
3, using hole tray earth culture method, the root system for being bred as seedling can rapidly adapt to field soil environment, and survival rate significantly improves.
Specific embodiment
The following examples can make those skilled in the art that the present invention be more fully understood, but not limit in any way
The present invention.
Embodiment 1
The implantation methods of Gynura divaricata, include the following steps：
1) the young sprout Gynura divaricata stem section that current year is raw is taken, blade is cut off and retains petiole, after irrigation and disinfection, is cut into 0.5-1.0cm
Stem with bud, be immersed in concentration be 0.8% fulvic acid solution in it is spare；
2) the Gynura divaricata plant that will obtain young sprout stem section scrapes epidermis after removing blade, after being crushed together with root,
Broken wall treatment, the raw material after broken wall are centrifugated after being mixed with 5 times of clear water, obtain a clear liquid, filter residue add 3 times it is clear
Water is centrifugated after being mixed, and obtains secondary clear liquid, clear liquid is mixed to get Gynura divaricata extracting solution twice；
3) it after step 2) being removed blade, epidermis and the crushing of the Gynura divaricata stubble of root, is centrifugated twice with step 2)
Filter residue mixing, mixture and peat, vermiculite, garden mould press 1:2:1:After 1 ratio mixing, by the Gynura divaricata extracting solution of step 2)
Water content after 5 times of dilution for adjusting mixture carries out sterilization treatment, obtains culture substrate to 60%；
4) stem with bud of step 1) is taken out, is embedded in the culture substrate of step 3), exposed portion is less than the 1/ of overall length
5, seedling rearing room is interior to keep whole day illumination, and carries out shading treatment with single layer shading net；
5) it when the water content of culture substrate is lower than 30%, is poured, is kept with 5 times of diluted Gynura divaricata extracting solutions
The water content of culture substrate is 50-60%；Completion nursery in 19 days, obtains basic seedling.
6) basic seedling is transplanted to crop field together with culture substrate, planting density is 2000 plants/acre, sprinkles profoundly water primary, use
The double-deck shading net blocks processing 3 days, then changes single layer shading net into and block processing 6 days, and ground moistening is kept during shading treatment, is hidden
Normal water and fertilizer management can be carried out after light processing.
Embodiment 2
The implantation methods of Gynura divaricata, include the following steps：
1) the young sprout Gynura divaricata stem section that current year is raw is taken, blade is cut off and retains petiole, after irrigation and disinfection, is cut into 0.5-1.0cm
Stem with bud, be immersed in concentration be 0.5% fulvic acid solution in it is spare；
2) the Gynura divaricata plant that will obtain young sprout stem section scrapes epidermis after removing blade, after being crushed together with root,
Broken wall treatment, the raw material after broken wall are centrifugated after being mixed with 3 times of clear water, obtain a clear liquid, filter residue add 3 times it is clear
Water is centrifugated after being mixed, and obtains secondary clear liquid, clear liquid is mixed to get Gynura divaricata extracting solution twice；
3) it after step 2) being removed blade, epidermis and the crushing of the Gynura divaricata stubble of root, is centrifugated twice with step 2)
Filter residue mixing, mixture and peat, vermiculite, garden mould press 1:2:1:After 1 ratio mixing, by the Gynura divaricata extracting solution of step 2)
Water content after 5-10 times of dilution for adjusting mixture carries out sterilization treatment, obtains culture substrate to 80%；
4) stem with bud of step 1) is taken out, is embedded in the culture substrate of step 3), exposed portion is less than the 1/ of overall length
5, seedling rearing room is interior to keep whole day illumination, and carries out shading treatment with single layer shading net；
5) it when the water content of culture substrate is lower than 30%, is poured, is kept with 7 times of diluted Gynura divaricata extracting solutions
The water content of culture substrate is 50-60%；Completion nursery in 18 days, obtains basic seedling.
6) basic seedling is transplanted to crop field together with culture substrate, planting density is 2000 plants/acre, sprinkles profoundly water primary, use
The double-deck shading net blocks processing 5 days, then changes single layer shading net into and block processing 5 days, during which holding ground moistening, after shading treatment
It can carry out normal water and fertilizer management.
Embodiment 3
The implantation methods of Gynura divaricata, include the following steps：
1) the young sprout Gynura divaricata stem section that current year is raw is taken, blade is cut off and retains petiole, after irrigation and disinfection, is cut into 0.5-1.0cm
Stem with bud, be immersed in concentration be 1% fulvic acid solution in it is spare；
2) the Gynura divaricata plant that will obtain young sprout stem section scrapes epidermis after removing blade, after being crushed together with root,
Broken wall treatment, the raw material after broken wall are centrifugated after being mixed with 5 times of clear water, obtain a clear liquid, filter residue add 2 times it is clear
Water is centrifugated after being mixed, and obtains secondary clear liquid, clear liquid is mixed to get Gynura divaricata extracting solution twice；
3) it after step 2) being removed blade, epidermis and the crushing of the Gynura divaricata stubble of root, is centrifugated twice with step 2)
Filter residue mixing, mixture and peat, vermiculite, garden mould press 1:2:1:After 1 ratio mixing, by the Gynura divaricata extracting solution of step 2)
Water content after 8 times of dilution for adjusting mixture carries out sterilization treatment, obtains culture substrate to 70%；
4) stem with bud of step 1) is taken out, is embedded in the culture substrate of step 3), exposed portion is less than the 1/ of overall length
5, seedling rearing room is interior to keep whole day illumination, and carries out shading treatment with single layer shading net；
5) it when the water content of culture substrate is lower than 30%, is poured, is kept with 8 times of diluted Gynura divaricata extracting solutions
The water content of culture substrate is 50-60%；Completion nursery in 15 days, obtains basic seedling.
6) basic seedling is transplanted to crop field together with culture substrate, planting density is 2500 plants/acre, sprinkles profoundly water primary, use
The double-deck shading net blocks processing 4 days, then changes single layer shading net into and block processing 7 days, during which holding ground moistening, after shading treatment
It can carry out normal water and fertilizer management.
Embodiment 4
The implantation methods of Gynura divaricata, include the following steps：
1) the young sprout Gynura divaricata stem section that current year is raw is taken, blade is cut off and retains petiole, after irrigation and disinfection, is cut into 0.5-1.0cm
Stem with bud, be immersed in concentration be 0.6% fulvic acid solution in it is spare；
2) the Gynura divaricata plant that will obtain young sprout stem section scrapes epidermis after removing blade, after being crushed together with root,
Broken wall treatment, the raw material after broken wall are centrifugated after being mixed with 4 times of clear water, obtain a clear liquid, filter residue add 3 times it is clear
Water is centrifugated after being mixed, and obtains secondary clear liquid, clear liquid is mixed to get Gynura divaricata extracting solution twice；
3) it after step 2) being removed blade, epidermis and the crushing of the Gynura divaricata stubble of root, is centrifugated twice with step 2)
Filter residue mixing, mixture and peat, vermiculite, garden mould press 1:2:1:After 1 ratio mixing, by the Gynura divaricata extracting solution of step 2)
Water content after 10 times of dilution for adjusting mixture carries out sterilization treatment, obtains culture substrate to 65%；
4) stem with bud of step 1) is taken out, is embedded in the culture substrate of step 3), exposed portion is less than the 1/ of overall length
5, seedling rearing room is interior to keep whole day illumination, and carries out shading treatment with single layer shading net；
5) it when the water content of culture substrate is lower than 30%, is poured, is protected with 10 times of diluted Gynura divaricata extracting solutions
The water content for holding culture substrate is 50-60%；Completion nursery in 18 days, obtains basic seedling.
6) basic seedling is transplanted to crop field together with culture substrate, planting density is 2000-2500 plants/acre, sprinkles profoundly water one
It is secondary, processing 4 days is blocked with the double-deck shading net, then change single layer shading net into and block processing 7 days, during which keeps ground moistening, shading
Normal water and fertilizer management can be carried out after processing.
Comparative example
1) the young sprout Gynura divaricata stem section that current year is raw is taken, blade is cut off and retains petiole, after irrigation and disinfection, is cut into 0.5-1.0cm
Stem with bud, be immersed in concentration be 0.6% fulvic acid solution in it is spare；
2) it after blade, epidermis and the crushing of the Gynura divaricata stubble of root will be removed, is centrifuged twice with step 2) in embodiment 4
Isolated filter residue mixing, mixture and peat, vermiculite, garden mould press 1:2:1:After 1 ratio mixing, mixture is adjusted with culture solution
Water content to 65%, carry out sterilization treatment, obtain culture substrate；The group of the culture solution becomes：MS+BA 1.5mg/L+NAA
1.2mg/L+ sucrose 2g/L+ active carbon 1g/L+ agar powder 6g/L；
3) stem with bud of step 1) is taken out, is embedded in the culture substrate of step 2), exposed portion is less than the 1/ of overall length
5, seedling rearing room is interior to keep whole day illumination, and carries out shading treatment with single layer shading net；
4) it when the water content of culture substrate is lower than 30%, is poured, is protected with 10 times of diluted Gynura divaricata extracting solutions
The water content for holding culture substrate is 50-60%；Completion nursery in 22 days, obtains basic seedling.
5) basic seedling is transplanted to crop field together with culture substrate, planting density is 2300 plants/acre, sprinkles profoundly water primary, use
The double-deck shading net blocks processing 4 days, then changes single layer shading net into and block processing 7 days, during which holding ground moistening, after shading treatment
It can carry out normal water and fertilizer management.
The case where pouring culture substrate nursery using method nursery of the invention and using existing culture medium is compared：
It will be understood by those skilled in the art that above embodiments are only exemplary embodiments, without departing substantially from spirit of the invention
In the case where range, a variety of variations can be carried out, replaced and changed.
Claims (3)
1. the implantation methods of Gynura divaricata, which is characterized in that include the following steps：
1) the young sprout Gynura divaricata stem section that current year is raw is taken, blade is cut off and retains petiole, after irrigation and disinfection, is cut into the band of 0.5-1.0cm
Leaf stem section is immersed in spare in the fulvic acid solution that concentration is 0.5-1%；
2) the Gynura divaricata plant that will obtain young sprout stem section scrapes epidermis after removing blade, after being crushed together with root, broken wall
Processing, the raw material after broken wall are centrifugated after being mixed with 3-5 times of clear water, obtain a clear liquid, filter residue add 2-3 times it is clear
Water is centrifugated after being mixed, and obtains secondary clear liquid, clear liquid is mixed to get Gynura divaricata extracting solution twice；
3) filter that after step 2) removal blade, epidermis and the Gynura divaricata stubble crushing of root, will be centrifugated twice with step 2)
Slag mixing, mixture and peat, vermiculite, garden mould press 1:2:1:After 1 ratio mixing, the Gynura divaricata extracting solution of step 2) is diluted
Water content after 5-10 times for adjusting mixture carries out sterilization treatment, obtains culture substrate to 60-80%；
4) stem with bud of step 1) is taken out, is embedded in the culture substrate of step 3), exposed portion is less than the 1/5 of overall length, educates
Whole day illumination is kept in seedling room, and carries out shading treatment with single layer shading net；
5) it when the water content of culture substrate is lower than 30%, is poured, is kept with 5-10 times of diluted Gynura divaricata extracting solution
The water content of culture substrate is 50-60%；Completion nursery in 15-20 days, obtains basic seedling.
2. the implantation methods of Gynura divaricata according to claim 1, which is characterized in that by basic transplantation of seedlings to crop field, plantation
Density is 2000-2500 plants/acre, is sprinkled profoundly water once, blocks processing 3-5 days with the double-deck shading net, then changes the screening of single layer shading net into
Gear is handled 5-7 days, is kept ground moistening during shading treatment, can be carried out normal water and fertilizer management after shading treatment.
3. the implantation methods of Gynura divaricata according to claim 1, which is characterized in that step 4), 5) be hole plate seedling growth, obtain
Basic seedling transplanted together with culture substrate.
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| CN110972835A (en) * | 2019-12-31 | 2020-04-10 | 贺州市长寿草农业开发有限公司 | Planting method of jonquil |
| CN113455365A (en) * | 2021-08-02 | 2021-10-01 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Method for preserving potted plant of common wild rice seed stems |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110972835A (en) * | 2019-12-31 | 2020-04-10 | 贺州市长寿草农业开发有限公司 | Planting method of jonquil |
| CN113455365A (en) * | 2021-08-02 | 2021-10-01 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Method for preserving potted plant of common wild rice seed stems |
| CN113455365B (en) * | 2021-08-02 | 2022-05-31 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Method for preserving potted plant of common wild rice seed stems |
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| CN108887110B (en) | 2020-11-17 |
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