CN108865154A - A kind of cadmium soil remediation biological agent - Google Patents
A kind of cadmium soil remediation biological agent Download PDFInfo
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Abstract
The invention discloses a kind of cadmium soil remediation biological agents, belong to technical field of soil remediation.The acid phosphatase enzymatic 2- phosphoglycerol hydrolysis of citric acid bacillus secretion is added in the present invention, and mixed fungus is added by compartmentation, has very strong intake ability to cadmium, reduces the toxic action to soil；Add potassium feldspar, montmorillonite etc., it is added in cadmium soil conditioner has duct exchange effect at room temperature, increase ion diffusion velocity and exchange capacity, promote the ion exchange of substance in cadmium and duct, pass through loading microorganisms inorganic agent simultaneously, microorganism carries out resolution process to cadmium ion in cellular structure, improves treatment effeciency；Low-acyl gellan gum, casein sodium are added again and forms glue network on inorganic agent surface, are guaranteed that the active material of cadmium soil remediation biological agent not easily runs off, are extended action time, improve the effect to conserve water and soil.The present invention solves the problems, such as that cadmium soil-repairing agent remediation efficiency is poor at present, it is short to repair validity period.
Description
Technical field
The present invention relates to technical field of soil remediation, and in particular to a kind of cadmium soil remediation biological agent.
Background technique
It is announced within 2014 according to Environmental Protection Department and Ministry of Land and Resources《National Soil Pollution Investigation bulletin》Display：The whole nation
Soil environment situation totally allows of no optimist, and some areas soil pollution is heavier, and arable soil environmental quality causes anxiety, and industry and mining are discarded
Ground soil environment outstanding problem.The human activities such as industry and mining, agricultural and Environmental Background Values of Soils height be cause soil pollution or
The main reason for exceeded.The total exceeding standard rate of national soil is 16.1%, wherein slightly, slightly, moderate and serious pollution point ratio
Respectively 11.2%, 2.3%, 1.5% and 1.1%.Based on inorganic type, organic type takes second place pollution type, compound pollution specific gravity compared with
Small, exceeded digit of inorganic pollution accounts for the 82.8% of all exceeded points.In terms of pollution distribution situation, soils in south china pollution weight
In the north；The partial regions such as Yangtze River Delta, the Delta of the Pearl River, Northeastern rust belt soil contamination problem is more prominent, west
South, variable charge soil heavy metals exceeding standard range are larger；4 kinds of cadmium, mercury, arsenic, lead level of inorganic contaminants distributions are presented from northwest
The situation gradually risen to the southeast, from northeast to southwestward.Major pollutants be cadmium, nickel, copper, arsenic, mercury, lead, DDT and
Polycyclic aromatic hydrocarbon.If being subject to second national land investigation result, China arable land latest data is 2,030,770,000 mu to calculate, in
The cultivated area of serious pollution accounts for 2.9%, has reached 58,890,000 mu.Heavy metal pollution is that current contaminated area is most wide, endangers most
One of big environmental problem, according to incompletely statistics, heavy metal pollution cultivated area nearly 2.0 × 108h ㎡ in China accounts for about total
The 1/5 of cultivated area, wherein being 1.33 × 104h ㎡ by cadmium pollution cultivated area.The cadmium pollution of soil not only influences crops
Growth and quality, but also will be entered in human body by the direct or indirect mode of food chain relation, it is strong to seriously threaten human body
Health.The heavy metal pollution of soil has concealment, chronicity and irreversibility, therefore, right in the contaminated situation in farmland
It is the key that prevent heavy metal pollution from influencing human health that the transfer enrichment behavior of soil-crop system pollution of trace, which carries out regulation,.Soil at present
And the status that heavy metal cadmium is got worse, the method for rehabilitating soil cadmium include biological restoration, agricultural regulation method, engineering
Physical method.Biological restoration is repaired thoroughly, but repairing efficiency is long；Agricultural regulation method is low for heavy metal pollution degree
Soil is effective and feasible, but then the effect is relatively poor for soil higher for heavy metal pollution degree；The cost of gongwu method is larger.
Summary of the invention
The technical problems to be solved by the invention：It is poor for current cadmium soil-repairing agent remediation efficiency, repair validity period it is short
The problem of, a kind of cadmium soil remediation biological agent is provided.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of cadmium soil remediation biological agent includes following component, 3 ~ 5 parts of soil adjuvants, 10 ~ 20 parts of quality according to parts by weight
The sodium chloride solution that score is 0.9% further includes 20 ~ 30 parts of biological passivator, 10 ~ 15 parts of mineral additives；
The preparation method of the biology passivator, includes the following steps：
（1）Take koning trichoderma bacterium, Rhizopus oryzae in mass ratio 1 ~ 3：2~4：2 ~ 5 are added Phanerochaete chrysosporium, obtain mixed fungus
Matrix object takes mixed fungus matrix object to be seeded in fungus culture medium by 3 ~ 7% inoculum concentration, cultivates in 120rpm, 25 ~ 30 DEG C,
Centrifugation takes precipitating in mass ratio 1：8 ~ 10 are added sterile saline, and oscillation obtains matrix liquid, takes citric acid bacillus by 3 ~ 5%
Inoculum concentration is seeded in TSB culture medium, is cultivated 2 ~ 3 days in 28 ~ 33 DEG C, and centrifugation takes precipitate A, is freeze-dried, must be freeze-dried
Object takes matrix liquid in mass ratio 8 ~ 10：3 ~ 5 are added freeze-drying object, obtain mixture；
（2）Take ferriferrous oxide nano-particle in mass ratio 1 ~ 3：8 ~ 12 are added sodium alginate soln, are stirred 30 ~ 40min,
Sterilizing, is cooled to room temperature, obtains cooled material, take mixture in mass ratio 1：3 ~ 5 be added cooled materials, obtain mixed liquor, take mixed liquor by
Mass ratio 3 ~ 6：4 ~ 8 are added dropwise in calcium chloride solution, stand, and filtering takes filter residue and drying to get biological passivator.
The step（1）Middle fungus culture medium：According to the mass fraction, 2 ~ 5 parts of potassium dihydrogen phosphates, 0.5 ~ 0.8 part are taken
MgSO4·7H2O, 0.01 ~ 0.04 part of VB1, 0.1 ~ 0.3 part of ammonium tartrate, 8 ~ 10 parts of glucose, 2 ~ 5 parts of liquid microelements, 1000
Part water adjusts pH value to 4.5 ~ 4.7,121 DEG C of 30 min of sterilizing with sodium tartrate buffer.
The liquid microelement is：According to the mass fraction, 1 ~ 3 part of sodium chloride, 0.15 ~ 0.2 part of CoCl are taken2·6H2O、0.01
~ 0.03 part of Na2MoO4·2H2O, 0.08 ~ 0.13 part of ZnSO4·7H2O, 0.09 ~ 0.15 part of CaCl2, 0.01 ~ 0.03 part of CuSO4·
5H2O, 0.3 ~ 0.6 part of MnSO4·H2O, 0.1 ~ 0.5 part of FeSO4·7H2O, 0.01 ~ 0.03 part of AlK (SO4)2·12H2O, 3 ~ 6 parts
MgSO4·7H2O, 0.01 ~ 0.03 part of boric acid, 1 ~ 3 part of chelating agent [S, S]-EDDS, 1000 parts of water, 121 DEG C of 30 min of sterilizing.
The mineral additive is：According to the mass fraction, 10 ~ 20 parts of potassium feldspars, 3 ~ 5 parts of kaolinites, 4 ~ 7 parts of montmorillonites, 7 ~ 9 are taken
Part lime stone, 50 ~ 60 parts of water, ball milling is dry, and heat preservation is cooled to room temperature to get mineral additive.
The heat-retaining condition of mineral additive is after the ball milling：It is warming up to 900 ~ 1000 DEG C of 1 ~ 2h of heat preservation.
The soil adjuvant is：Sodium caseinate powder in mass ratio 1：10 ~ 15 are added deionized water, in 25 ~ 30 DEG C of magnetic
Power stirs 3 ~ 5h, obtains stirring mixture, takes low-acyl gellan gum powder in mass ratio 1：5 ~ 8 are added deionized water, molten at room temperature
Swollen 18 ~ for 24 hours, it is stirred 2 ~ 3h in 75 ~ 80 DEG C, mixture A must be stirred, takes stirring mixture in mass ratio 2 ~ 4：5 ~ 8 are added
Stirring mixture A is stirred to get soil adjuvant.
Cadmium soil remediation biological agent according to claim 1, which is characterized in that the stirring mixture and stirring
Mixture A mixing condition is：40 ~ 50min is stirred in 65 ~ 70 DEG C.
The present invention is compared with other methods, and advantageous effects are：
（1）The acid phosphatase enzymatic 2- phosphoglycerol hydrolysis of citric acid bacillus secretion is added in the present invention, makes a large amount of inorganic phosphates
Salt generates and is enriched in microbial cell surface, and precipitation reaction occurs for the cadmium ion in inorganic phosphate and soil, so that
Cadmium metal is passivated, and Rhizopus oryzae, Phanerochaete chrysosporium is added, contains mannosan, glucan, chitin in cell wall
The ingredients such as matter, these substances have stronger negative electrical charge, can adsorb Cd2+, cadmium ion is deposited on its cell wall, cell wall
It is upper that there are the functional groups such as amino, carboxyl, hydroxyl, mixed fungus is added by compartmentation, passes through cell membrane transport to bacterium ball
Inside is by Cd2+It transports intracellular, accumulates in the cell, therefore have very strong intake ability to cadmium, reduce the poison to soil
Evil effect；
（2）The present invention adds potassium feldspar, montmorillonite etc., and the ducts such as some of sodium, potassium and calcium cation can be with Cd2+Occur
Ion exchange generates cadmium feldspar object phase, and be added in cadmium soil conditioner has duct exchange effect at room temperature, and due to Cd2+
Radius and K+、Na+Plasma differs smaller, Cd2+It can be with K in duct+、Na+Plasma swaps, and enters through heating and protects
Temperature, cellular structure generate lead feldspar, and the retractility of skeleton improves, and hole increases, while the cation acquisition in framework is higher
Energy increases ion diffusion velocity and exchange capacity, so that Cd2+More easily enter mineral structure, promotes in cadmium and duct
The ion exchange of substance, while by loading microorganisms inorganic agent, microorganism carries out at decomposition cadmium ion in cellular structure
Reason, improves treatment effeciency；
（3）The present invention adds low-acyl gellan gum, casein sodium and forms glue network, the carboxylic of gellan gum molecule on inorganic agent surface
Base side chain is mutually exclusive due to electrostatic interaction, hinders the tight clusters of double helix, and intervention cationic in system can shield
Covering electrostatic repulsion, cadmium bivalent cation links the carboxyl on glucose residue by ionic bond, framework double-spiral structure, from
And three-dimensional net structure is formed, active material is on the one hand sticked by gel network, guarantees the work of cadmium soil remediation biological agent
Property substance not easily run off, extend action time, on the other hand have water conservation permeation functions to soil, improve the effect to conserve water and soil
Fruit.
Specific embodiment
Koning trichoderma bacterium, Rhizopus oryzae, Phanerochaete chrysosporium, citric acid bacillus：In 2 ~ 4 DEG C of laboratory slant preservations.
Fungus culture medium：According to the mass fraction, 2 ~ 5 parts of potassium dihydrogen phosphates, 0.5 ~ 0.8 part of MgSO are taken4·7H2O、0.01~
0.04 part of VB1, 0.1 ~ 0.3 part of ammonium tartrate, 8 ~ 10 parts of glucose, 2 ~ 5 parts of liquid microelements, 1000 parts of water, be 20 with concentration
The sodium tartrate buffer of mmol/L adjusts pH value to 4.5 ~ 4.7,121 DEG C of 30 min of sterilizing.
Liquid microelement：According to the mass fraction, 1 ~ 3 part of sodium chloride, 0.15 ~ 0.2 part of CoCl are taken2·6H2O、0.01~0.03
Part Na2MoO4·2H2O, 0.08 ~ 0.13 part of ZnSO4·7H2O, 0.09 ~ 0.15 part of CaCl2, 0.01 ~ 0.03 part of CuSO4·5H2O、
0.3 ~ 0.6 part of MnSO4·H2O, 0.1 ~ 0.5 part of FeSO4·7H2O, 0.01 ~ 0.03 part of AlK (SO4)2·12H2O, 3 ~ 6 parts
MgSO4·7H2O, 0.01 ~ 0.03 part of boric acid, 1 ~ 3 part of chelating agent [S, S]-EDDS, 1000 parts of water, 121 DEG C of 30 min of sterilizing.
Biological passivator：（1）Take koning trichoderma bacterium, Rhizopus oryzae in mass ratio 1 ~ 3：2~4：2 ~ 5 are added Phanerochaete chrysosporium,
Mixed fungus matrix object is obtained, takes mixed fungus matrix object to be seeded in fungus culture medium by 3 ~ 7% inoculum concentration, in 120rpm, 25
~ 30 DEG C are cultivated 3 ~ 5 days, and centrifugation takes precipitating in mass ratio 1：8 ~ 10 are added sterile saline, vibrate 30 ~ 40min, obtain matrix
Liquid takes citric acid bacillus to be seeded in TSB culture medium by 3 ~ 5% inoculum concentration, cultivates 2 ~ 3 days in 28 ~ 33 DEG C, and centrifugation takes precipitating
A, freeze-drying, obtains freeze-drying object, takes matrix liquid in mass ratio 8 ~ 10：3 ~ 5 are added freeze-drying object, obtain mixture；
（2）Take ferriferrous oxide nano-particle in mass ratio 1 ~ 3：8 ~ 12 are added the sodium alginate soln that mass fraction is 5%, stir
30 ~ 40min of mixing is mixed, in 121 DEG C of 20 min of sterilizing, is cooled to room temperature, obtains cooled material, take mixture in mass ratio 1：3 ~ 5 add
Enter cooled material, obtain mixed liquor, takes mixed liquor in mass ratio 3 ~ 6：4 ~ 8 are added dropwise in the calcium chloride solution that concentration is 0.2mol/L,
Control time for adding is 30 ~ 40min, stands 2 ~ 3h, and filtering takes filter residue and drying to get biological passivator.
Mineral additive：According to the mass fraction, 10 ~ 20 parts of potassium feldspars, 3 ~ 5 parts of kaolinites, 4 ~ 7 parts of montmorillonites, 7 ~ 9 parts are taken
Lime stone, 50 ~ 60 parts of water, ball milling is dry, is warming up to 900 ~ 1000 DEG C of 1 ~ 2h of heat preservation, is cooled to room temperature to get mineral addition
Agent.
Soil adjuvant：Sodium caseinate powder in mass ratio 1：10 ~ 15 are added deionized water, in 25 ~ 30 DEG C of magnetic agitations 3
~ 5h obtains stirring mixture, takes low-acyl gellan gum powder in mass ratio 1：5 ~ 8 be added deionized waters, at room temperature be swollen 18 ~
For 24 hours, it is stirred 2 ~ 3h in 75 ~ 80 DEG C, mixture A must be stirred, take stirring mixture in mass ratio 2 ~ 4：5 ~ 8, which are added stirring, mixes
Object A mixing is closed, is stirred 40 ~ 50min in 65 ~ 70 DEG C to get soil adjuvant.
A kind of cadmium soil remediation biological agent takes 20 ~ 30 parts of biological passivator, 10 ~ 15 parts of mineral to add according to the mass fraction
The sodium chloride solution that agent, 3 ~ 5 parts of soil adjuvants, 10 ~ 20 parts of mass fractions are 0.9% is added to mix to get biological agent.
Koning trichoderma bacterium, Rhizopus oryzae, Phanerochaete chrysosporium, citric acid bacillus：In 2 DEG C of laboratory slant preservations.
Fungus culture medium：According to the mass fraction, 2 parts of potassium dihydrogen phosphates, 0.5 part of MgSO are taken4·7H2O, 0.01 part of VB1、0.1
Part ammonium tartrate, 8 parts of glucose, 2 parts of liquid microelements, 1000 parts of water, the sodium tartrate buffer for being 20 mmol/L with concentration
PH value is adjusted to 4.5,121 DEG C of 30 min of sterilizing.
Liquid microelement：According to the mass fraction, 1 part of sodium chloride, 0.15 part of CoCl are taken2·6H2O, 0.01 part of Na2MoO4·
2H2O, 0.08 part of ZnSO4·7H2O, 0.09 part of CaCl2, 0.01 part of CuSO4·5H2O, 0.3 part of MnSO4·H2O, 0.1 part
FeSO4·7H2O, 0.01 part of AlK (SO4)2·12H2O, 3 parts of MgSO4·7H2O, 0.01 part of boric acid, 1 part of chelating agent [S, S]-
EDDS, 1000 parts of water, 121 DEG C of 30 min of sterilizing.
Biological passivator：（1）Take koning trichoderma bacterium, Rhizopus oryzae in mass ratio 1：2：2 are added Phanerochaete chrysosporium, must mix
Fungi matrix object, takes mixed fungus matrix object to be seeded in fungus culture medium by 3% inoculum concentration, in 120rpm, 25 DEG C of cultures 3
It, centrifugation takes precipitating in mass ratio 1：8 are added sterile saline, vibrate 30min, obtain matrix liquid, take citric acid bacillus by 3%
Inoculum concentration be seeded in TSB culture medium, in 28 DEG C cultivate 2 days, centrifugation, take precipitate A, be freeze-dried, obtain freeze-drying object, take
Matrix liquid in mass ratio 8：3 are added freeze-drying object, obtain mixture；
（2）Take ferriferrous oxide nano-particle in mass ratio 1：8 are added the sodium alginate soln that mass fraction is 5%, are stirred
30min is cooled to room temperature in 121 DEG C of 20 min of sterilizing, obtains cooled material, take mixture in mass ratio 1：3 are added cooled material, obtain
Mixed liquor takes mixed liquor in mass ratio 3：4 are added dropwise in the calcium chloride solution that concentration is 0.2mol/L, and control time for adding is
30min stands 2h, mistakeFilter, take filter residue and drying to get biological passivator.
Mineral additive：According to the mass fraction, 10 parts of potassium feldspars, 3 parts of kaolinites, 4 parts of montmorillonites, 7 parts of lime stones are taken, 50
Part water, ball milling is dry, is warming up to 900 DEG C of heat preservation 1h, is cooled to room temperature to get mineral additive.
Soil adjuvant：Sodium caseinate powder in mass ratio 1：10 addition deionized waters must be stirred in 25 DEG C of magnetic agitation 3h
Mixture is mixed, low-acyl gellan gum powder in mass ratio 1 is taken：5 are added deionized water, are swollen 18h at room temperature, mixed in 75 DEG C of stirrings
2h is closed, mixture A must be stirred, takes stirring mixture in mass ratio 2：5 are added stirring mixture A mixing, are stirred in 65 DEG C
40min is to get soil adjuvant.
A kind of cadmium soil remediation biological agent takes 20 parts of biological passivator, 10 parts of mineral additives, 3 according to the mass fraction
The sodium chloride solution that part soil adjuvant, 10 parts of mass fractions are 0.9% mixes to get biological agent.
Koning trichoderma bacterium, Rhizopus oryzae, Phanerochaete chrysosporium, citric acid bacillus：In 3 DEG C of laboratory slant preservations.
Fungus culture medium：According to the mass fraction, 4 parts of potassium dihydrogen phosphates, 0.7 part of MgSO are taken4·7H2O, 0.03 part of VB1、0.2
Part ammonium tartrate, 9 parts of glucose, 4 parts of liquid microelements, 1000 parts of water, the sodium tartrate buffer for being 20 mmol/L with concentration
PH value is adjusted to 4.6,121 DEG C of 30 min of sterilizing.
Liquid microelement：According to the mass fraction, 2 parts of sodium chloride, 0.18 part of CoCl are taken2·6H2O, 0.02 part of Na2MoO4·
2H2O, 0.11 part of ZnSO4·7H2O, 0.12 part of CaCl2, 0.02 part of CuSO4·5H2O, 0.5 part of MnSO4·H2O, 0.3 part
FeSO4·7H2O, 0.02 part of AlK (SO4)2·12H2O, 5 parts of MgSO4·7H2O, 0.02 part of boric acid, 2 parts of chelating agents [S, S]-
EDDS, 1000 parts of water, 121 DEG C of 30 min of sterilizing.
Biological passivator：（1）Take koning trichoderma bacterium, Rhizopus oryzae in mass ratio 2：3：4 are added Phanerochaete chrysosporium, must mix
Fungi matrix object, takes mixed fungus matrix object to be seeded in fungus culture medium by 5% inoculum concentration, in 120rpm, 28 DEG C of cultures 4
It, centrifugation takes precipitating in mass ratio 1：9 are added sterile saline, vibrate 35min, obtain matrix liquid, take citric acid bacillus by 4%
Inoculum concentration be seeded in TSB culture medium, in 30 DEG C cultivate 2.5 days, centrifugation, take precipitate A, be freeze-dried, obtain freeze-drying object,
Take matrix liquid in mass ratio 9：4 are added freeze-drying object, obtain mixture；
（2）Take ferriferrous oxide nano-particle in mass ratio 2：10 are added the sodium alginate soln that mass fraction is 5%, and stirring is mixed
35min is closed to be cooled to room temperature in 121 DEG C of 20 min of sterilizing, obtain cooled material, take mixture in mass ratio 1：4 are added cooled material,
Mixed liquor is obtained, mixed liquor in mass ratio 5 is taken：6 are added dropwise in the calcium chloride solution that concentration is 0.2mol/L, and control time for adding is
35min, stands 2.5h, and filtering takes filter residue and drying to get biological passivator.
Mineral additive：According to the mass fraction, 15 parts of potassium feldspars, 4 parts of kaolinites, 6 parts of montmorillonites, 8 parts of lime stones are taken, 55
Part water, ball milling is dry, is warming up to 950 DEG C of heat preservation 1.5h, is cooled to room temperature to get mineral additive.
Soil adjuvant：Sodium caseinate powder in mass ratio 1：13 addition deionized waters must be stirred in 28 DEG C of magnetic agitation 4h
Mixture is mixed, low-acyl gellan gum powder in mass ratio 1 is taken：7 are added deionized water, are swollen 21h at room temperature, mixed in 78 DEG C of stirrings
2.5h is closed, mixture A must be stirred, takes stirring mixture in mass ratio 3：7 are added stirring mixture A mixing, mixed in 68 DEG C of stirrings
45min is closed to get soil adjuvant.
A kind of cadmium soil remediation biological agent takes 25 parts of biological passivator, 13 parts of mineral additives, 4 according to the mass fraction
The sodium chloride solution that part soil adjuvant, 15 parts of mass fractions are 0.9% mixes to get biological agent.Embodiment 3
Koning trichoderma bacterium, Rhizopus oryzae, Phanerochaete chrysosporium, citric acid bacillus：In 4 DEG C of laboratory slant preservations.
Fungus culture medium：According to the mass fraction, 5 parts of potassium dihydrogen phosphates, 0.8 part of MgSO are taken4·7H2O, 0.04 part of VB1、0.3
Part ammonium tartrate, 10 parts of glucose, 5 parts of liquid microelements, 1000 parts of water are buffered with the sodium tartrate that concentration is 20 mmol/L
Liquid adjusts pH value to 4.7,121 DEG C of 30 min of sterilizing.
Liquid microelement：According to the mass fraction, 3 parts of sodium chloride, 0.2 part of CoCl are taken2·6H2O, 0.03 part of Na2MoO4·
2H2O, 0.13 part of ZnSO4·7H2O, 0.15 part of CaCl2, 0.03 part of CuSO4·5H2O, 0.6 part of MnSO4·H2O, 0.5 part
FeSO4·7H2O, 0.03 part of AlK (SO4)2·12H2O, 6 parts of MgSO4·7H2O, 0.03 part of boric acid, 3 parts of chelating agents [S, S]-
EDDS, 1000 parts of water, 121 DEG C of 30 min of sterilizing.
Biological passivator：（1）Take koning trichoderma bacterium, Rhizopus oryzae in mass ratio 3：4：5 are added Phanerochaete chrysosporium, must mix
Fungi matrix object, takes mixed fungus matrix object to be seeded in fungus culture medium by 7% inoculum concentration, in 120rpm, 30 DEG C of cultures 5
It, centrifugation takes precipitating in mass ratio 1：10 be added sterile salines, vibrate 40min, obtain matrix liquid, take citric acid bacillus by
5% inoculum concentration is seeded in TSB culture medium, is cultivated 3 days in 33 DEG C, and centrifugation takes precipitate A, is freeze-dried, is obtained freeze-drying object,
Take matrix liquid in mass ratio 10：5 are added freeze-drying object, obtain mixture；
（2）Take ferriferrous oxide nano-particle in mass ratio 3：12 are added the sodium alginate soln that mass fraction is 5%, and stirring is mixed
40min is closed to be cooled to room temperature in 121 DEG C of 20 min of sterilizing, obtain cooled material, take mixture in mass ratio 1：5 are added cooled material,
Mixed liquor is obtained, mixed liquor in mass ratio 6 is taken：8 are added dropwise in the calcium chloride solution that concentration is 0.2mol/L, and control time for adding is
40min, stands 3h, and filtering takes filter residue and drying to get biological passivator.
Mineral additive：According to the mass fraction, 20 parts of potassium feldspars, 5 parts of kaolinites, 7 parts of montmorillonites, 9 parts of lime stones are taken, 60
Part water, ball milling is dry, is warming up to 1000 DEG C of heat preservation 2h, is cooled to room temperature to get mineral additive.
Soil adjuvant：Sodium caseinate powder in mass ratio 1：15 addition deionized waters must be stirred in 30 DEG C of magnetic agitation 5h
Mixture is mixed, low-acyl gellan gum powder in mass ratio 1 is taken：8 are added deionized water, are swollen at room temperature for 24 hours, mixed in 80 DEG C of stirrings
3h is closed, mixture A must be stirred, takes stirring mixture in mass ratio 4：8 are added stirring mixture A mixing, are stirred in 70 DEG C
50min is to get soil adjuvant.
A kind of cadmium soil remediation biological agent takes 30 parts of biological passivator, 15 parts of mineral additives, 5 according to the mass fraction
The sodium chloride solution that part soil adjuvant, 20 parts of mass fractions are 0.9% mixes to get biological agent.
Comparative example 1：It is essentially identical with the preparation method of embodiment 3, it has only the difference is that lacking biological passivator.
Comparative example 2：It is essentially identical with the preparation method of embodiment 3, it has only the difference is that lacking mineral additive.
Comparative example 3：The making biological coal of Xingtai City company of Hebei province production.
Soil remediation biological agent prepared by embodiment 1,2,3 and comparative example 1,2,3 is uniformly mixed and is sprinkling upon heavy metal cadmium
The surface of contaminated soil is per acre 200kg, and then carrying out Soil tillage keeps soil-repairing agent and heavy metal cadmium soil abundant
It is uniformly mixed, balances 15 days, 30 days completion heavy metal-polluted soil repair processes.According to solid waste《Leaching leaching method sulphur
Acid+nitric acid method》(HJ/T299-2007) leaching test is carried out to soil using turnover oscillator, the soil huge sum of money after detection reparation
Belong to concentration, test result is recorded in table 1.
Table 1：
| Test item | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Soil-repairing agent pH value | 8.03 | 8.04 | 8.05 | 8.60 | 8.61 | 8.62 |
| The available state concentration of Cadmium in Soil before handling（ug/L） | 0.4390 | 0.4390 | 0.4390 | 0.4390 | 0.4390 | 0.4390 |
| The available state concentration of Cadmium in Soil after processing 15 days（ug/L） | It is not detected | It is not detected | It is not detected | 0.2546 | 0.2781 | 0.2436 |
| The available state concentration of Cadmium in Soil after processing 30 days（ug/L） | 0.0475 | 0.0343 | 0.0241 | 0.3526 | 0.2978 | 0.2956 |
In conclusion as shown in Table 1, soil remediation biological agent remediation efficiency of the invention greatly enhances, and effective time is long,
It is worth advocating energetically.
Claims (7)
1. a kind of cadmium soil remediation biological agent includes following component, 3 ~ 5 parts of soil adjuvants, 10 ~ 20 parts of matter according to parts by weight
Measure the sodium chloride solution that score is 0.9%, which is characterized in that further include 20 ~ 30 parts of biological passivator, 10 ~ 15 parts of mineral additions
Agent；
The preparation method of the biology passivator, includes the following steps：
（1）Take koning trichoderma bacterium, Rhizopus oryzae in mass ratio 1 ~ 3：2~4：2 ~ 5 are added Phanerochaete chrysosporium, obtain mixed fungus
Matrix object takes mixed fungus matrix object to be seeded in fungus culture medium by 3 ~ 7% inoculum concentration, cultivates in 120rpm, 25 ~ 30 DEG C,
Centrifugation takes precipitating in mass ratio 1：8 ~ 10 are added sterile saline, and oscillation obtains matrix liquid, takes citric acid bacillus by 3 ~ 5%
Inoculum concentration is seeded in TSB culture medium, is cultivated 2 ~ 3 days in 28 ~ 33 DEG C, and centrifugation takes precipitate A, is freeze-dried, must be freeze-dried
Object takes matrix liquid in mass ratio 8 ~ 10：3 ~ 5 are added freeze-drying object, obtain mixture；
（2）Take ferriferrous oxide nano-particle in mass ratio 1 ~ 3：8 ~ 12 are added sodium alginate soln, are stirred 30 ~ 40min,
Sterilizing, is cooled to room temperature, obtains cooled material, take mixture in mass ratio 1：3 ~ 5 be added cooled materials, obtain mixed liquor, take mixed liquor by
Mass ratio 3 ~ 6：4 ~ 8 are added dropwise in calcium chloride solution, stand, and filtering takes filter residue and drying to get biological passivator.
2. cadmium soil remediation biological agent according to claim 1, which is characterized in that the step（1）Middle fungal culture
Liquid：According to the mass fraction, 2 ~ 5 parts of potassium dihydrogen phosphates, 0.5 ~ 0.8 part of MgSO are taken4·7H2O, 0.01 ~ 0.04 part of VB1、0.1~0.3
Part ammonium tartrate, 8 ~ 10 parts of glucose, 2 ~ 5 parts of liquid microelements, 1000 parts of water, with sodium tartrate buffer adjust pH value to
4.5 ~ 4.7,121 DEG C of 30 min of sterilizing.
3. cadmium soil remediation biological agent according to claim 2, which is characterized in that the liquid microelement is：By matter
Number meter is measured, 1 ~ 3 part of sodium chloride, 0.15 ~ 0.2 part of CoCl are taken2·6H2O, 0.01 ~ 0.03 part of Na2MoO4·2H2O、0.08~0.13
Part ZnSO4·7H2O, 0.09 ~ 0.15 part of CaCl2, 0.01 ~ 0.03 part of CuSO4·5H2O, 0.3 ~ 0.6 part of MnSO4·H2O、0.1~
0.5 part of FeSO4·7H2O, 0.01 ~ 0.03 part of AlK (SO4)2·12H2O, 3 ~ 6 parts of MgSO4·7H2O, 0.01 ~ 0.03 part of boric acid, 1
~ 3 parts of chelating agent [S, S]-EDDS, 1000 parts of water, 121 DEG C of 30 min of sterilizing.
4. cadmium soil remediation biological agent according to claim 1, which is characterized in that the mineral additive is：By matter
Number meter is measured, takes 10 ~ 20 parts of potassium feldspars, 3 ~ 5 parts of kaolinites, 4 ~ 7 parts of montmorillonites, 7 ~ 9 parts of lime stones, 50 ~ 60 parts of water, ball milling,
Dry, heat preservation is cooled to room temperature to get mineral additive.
5. cadmium soil remediation biological agent according to claim 4, which is characterized in that mineral additive after the ball milling
Heat-retaining condition is：It is warming up to 900 ~ 1000 DEG C of 1 ~ 2h of heat preservation.
6. cadmium soil remediation biological agent according to claim 1, which is characterized in that the soil adjuvant is：Casein
Sour sodium powder end in mass ratio 1：10 ~ 15 addition deionized waters obtain stirring mixture in 25 ~ 30 DEG C of 3 ~ 5h of magnetic agitation, take low acyl
Base junction cold glue powder in mass ratio 1：5 ~ 8 be added deionized waters, at room temperature be swollen 18 ~ for 24 hours, be stirred 2 ~ 3h in 75 ~ 80 DEG C,
Mixture A must be stirred, stirring mixture in mass ratio 2 ~ 4 is taken：5 ~ 8 addition stirring mixture A are stirred to be helped to get soil
Agent.
7. cadmium soil remediation biological agent according to claim 1, which is characterized in that the stirring mixture and stirring are mixed
Closing object A mixing condition is：40 ~ 50min is stirred in 65 ~ 70 DEG C.
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Application publication date: 20181123 |