CN108853484B

CN108853484B - Method for inducing replacement gilts to estrus synchronously

Info

Publication number: CN108853484B
Application number: CN201810813305.2A
Authority: CN
Inventors: 刘忠华; 翁晓刚; 张宇霆; 刘龑
Original assignee: Harbin Aopu Kenong Biotechnology Co ltd; Northeast Agricultural University
Current assignee: Harbin Aopu Kenong Biotechnology Co ltd; Northeast Agricultural University
Priority date: 2018-07-23
Filing date: 2018-07-23
Publication date: 2021-08-17
Anticipated expiration: 2038-07-23
Also published as: CN108853484A

Abstract

The invention discloses a method for inducing a replacement gilt to estrus synchronously, belongs to the field of animal induced estrus and artificial insemination, and provides a method for inducing the replacement gilt to estrus synchronously aiming at the problem that the replacement gilt is difficult to estrus synchronously. The invention is beneficial to the large-scale sow production.

Description

Method for inducing replacement gilts to estrus synchronously

Technical Field

The invention relates to the field of animal induced estrus and artificial insemination, in particular to a processing method for inducing the synchronous estrus of sows, belonging to the technical field of livestock breeding.

Background

The estrus synchronization technology is one of the important reproduction control technologies in modern livestock production, and is characterized by utilizing certain hormone preparations to artificially control and regulate the estrus cycle of a group of female animals, so that the female animals can be subjected to centralized estrus, centralized mating, centralized pregnancy and centralized parturition, and the tissue production and management are facilitated. In addition, the estrus synchronization technology is beneficial to the further popularization of the artificial insemination technology and is also an important link of the embryo transplantation work. The estrus synchronization technology is widely applied to livestock breeding and achieves certain effect. Further research in this experiment will provide theoretical and technical support for production applications.

The phenomenon of the reproductive cycle exhibited by sexually mature female mammals during a particular season, physiologically manifested as ovulation, preparation for fertilization and pregnancy, and behaviourally manifested as attraction and acceptance of the opposite sex. The medicine for simultaneous estrus is mainly characterized by that it utilizes exogenous hormone to stimulate ovary, and makes it change according to the preset requirements, so that the physiological functions of ovary of female animal are in the same stage, so that it can attain the goal of synchronous estrus.

Estrus synchronization generally takes two approaches. One approach is to extend the luteal phase, administer progestogenic drugs to a group of dams simultaneously, and suppress growth and oestrus expression of the follicles. After a certain period of administration treatment, the medicine is stopped, and the ovary loses the control of exogenous progestational hormone, so that the periodic corpus luteum on the ovary is degenerated, and the follicle develops at the same time to cause the heat of the female animals. The progestational hormone is adopted to inhibit the oestrus of the female animals, actually, the luteal phase is artificially prolonged, and the effects of prolonging the oestrus cycle and delaying the oestrus are achieved. Another approach is to shorten the luteal phase, which is applied to accelerate luteal degeneration. The ovary gets rid of the control of the progestogen in the body in advance, so that the follicle can start to develop simultaneously, thereby achieving the synchronous estrus of the female animal. This actually shortens the oestrus cycle of the female animals and encourages the oestrus of the female animals in a short time. The hormones used in the two ways have different properties, but both have a regulating effect on the luteal phase, so that the luteal phase is prolonged or shortened, and finally the aim of regulating the ovarian function is fulfilled.

However, the existing replacement pig simultaneous estrus technology has a series of problems.

1. Because of the asynchrony of the oestrus of the replacement pigs, the pigs cannot be subjected to large-scale timed insemination, which is a great waste of labor force.

2. The existing methods for inducing synchronous estrus are very numerous, and particularly, some medicines are few in the market or rely on import, so that the cost of breeding pigs is increased invisibly and large-scale breeding cannot be realized.

3. At present, most of feeding methods of the progesterone analogues are added into daily feeds, and the pigs are unwilling to eat due to peculiar smell of the progesterone analogues and other medicines, so that the medicines cannot be all eaten by the pigs, and the oestrus in the later period cannot be synchronized due to great change of the medicine amount eaten by the pigs.

Disclosure of Invention

The method only aims at the replacement pig, and corresponding medicines are given at regular time and quantity, so that the induced and treated pig synchronously estruses in the preset time, and large-scale pig breeding and breeding are facilitated.

The sows treated by the method are required to be healthy and disease-free, particularly cannot carry diseases related to reproduction, and are aged 220-230 days, 130-140 kg of body weight and moderate in fat condition. The method uses three medicines: altrenogest, hCG, and eCG. Altrenogest is a progesterone analogue with the functions of prolonging luteal phase and inhibiting growth, development and oestrus expression of follicles. After a certain period of treatment, the medicine is stopped at the same time, and because the ovary loses the control of exogenous progestogen, the cycle corpus luteum on the ovary is degenerated, and then follicle development occurs at the same time, thereby causing the heat of the female animals. The hCG injection is a hormone medicine, and can promote the anterior pituitary of animals to release follicle stimulating hormone FSH and LH after injection, further cause the obvious rise of plasma LH and the slight rise of FSH, and promote the mature ovulation of oocytes of ovaries of female animals. eCG for injection has follicle stimulating and luteinizing effects, and can promote follicle maturation, ovulation and corpus luteum generation of female livestock, and stimulate corpus luteum to secrete progestogen.

According to the method for inducing the sows to estrus synchronously, each pig is fed with the altrenogest at a fixed point every day, after the pigs are continuously fed for 18-20 days and after an interval of 42-48 hours, each pig is injected with equine chorionic gonadotropin (eCG), after an interval of 80-85 hours, each pig is injected with human chorionic gonadotropin (hCG), and after an interval of 24 hours, the pigs are estrus uniformly. The sign of estrus takes stable receiving of the climbing of boars or other pigs as the main feature of estrus, and also comprises some behaviors of remarkably descending appetite, lying in time, climbing walls, arching, jumping on a fence and the like. Most importantly, the ovaries of the heat-induced pigs were ovulated and red bodies appeared as confirmed by surgical procedures.

Preferably, the pigs are fed altrenogest for 18 consecutive days, with an interval of 42h followed by injection of eCG and an interval of 80h followed by injection of hCG.

Further defined, the daily fixed-point allylogest feeding amount of each pig is 16-20 mg.

Further defined, the amount of eCG injected per pig is 400 IU.

Further defined, the amount of hCG injected into each pig is 200 IU.

Further limiting, the day age of the replacement gilt is 220 days to 230 days, and the weight of the replacement gilt is 130kg to 140 kg.

Further limiting, artificial insemination is performed after the pigs are estrated, estrus return does not occur within 21 days, and the treated pigs are pregnant and enter the gestation period.

Further limiting, the artificial insemination times are two times, namely 24h and 40h after the pig is in estrus.

Advantageous effects

1. The medicine used in the experiment has wide source and convenient use, and is beneficial to large-scale popularization and use.

2. The liquid medicine of the altrenogest in the method is colorless and tasteless, and can be directly taken by pigs by a feeding gun, so that the waste of medicine administration and the loss of dosage during administration are avoided.

3. The treated pigs all estrate in the same day after the hCG treatment, and the estrus induction efficiency is high.

4. The invention can improve the mating rate and the conception rate of the recipient sow, shorten the mating period, reduce the infertility, improve the reproduction rate and reduce the consumption of manpower and material resources, is beneficial to the development of the artificial insemination technology on the sow, and is convenient for large-scale tissue production and timed large-scale experiments; secondly, induction of synchronous estrus in batch production management of sows can reduce abnormal phenomena of the sows in the breeding process, bring great economic benefit to a pig farm, be beneficial to saving labor force, organizing large-scale production, really realizing full-in and full-out and being beneficial to leading swinery to reach the same breeding state, health state and immune state.

5. The invention is beneficial to the accurate and timed estrus of embryo transplantation acceptors and is beneficial to improving the embryo transplantation efficiency and success rate.

Drawings

Figure 1 example 1 treatment of ovarian ovulation in pigs.

Figure 2 example 2 treatment of ovarian ovulation in pigs.

Figure 3 example 3 treatment of ovarian ovulation in pigs.

Detailed Description

Example 1. method of inducing estrus in replacement gilts contemporaneously.

Selecting 18 replacement sows which are healthy and have no disease, particularly can not carry reproduction-related diseases, are aged for 220 days, have the weight of 130kg and are fat in moderate conditions.

After selecting pigs, feeding 16mg of altrenogest at a specified time, such as 14:00 pm per day, for 18 days, up to 20 days; at an interval of 48 hours after the last feeding, 400IU eCG was injected at 14:00 on day 20; the interval is 80 hours; injection of 200IU hCG at 22:00 on day 23; only the 22:00 pigs on day 24 began oestrous.

And (3) synchronous estrus detection: the operation laparotomy examination of the induced pig in this example shows that the ovaries are ovulated and red bodies appear, and as shown in fig. 1, the reproductive system of the pig develops well without any lesion. Both ovaries developed well and had ovulated and appeared as red bodies, with 11 red bodies in the left (shown as a in fig. 1) and 3 red bodies in the right (shown as B in fig. 1) of the pigs.

Artificial insemination: artificial insemination is respectively carried out twice at 22:00 of 25 th day and 13:00 of 26 th day, and heat check is carried out twice every day, if no heat return exists in 21 days, the treated pigs are successfully fertilized, and enter the gestation period, and all the pigs are successfully fertilized through heat check.

Example 2. method of inducing estrus in replacement gilts contemporaneously.

Selecting 18 replacement sows which are healthy and have no disease, particularly can not carry reproduction-related diseases, are 225 days old, have the weight of 135kg and are at medium fat condition.

After selecting pigs, feeding 18mg of altrenogest for 19 days at a specified time, such as 14:00 pm each day; at 45-hour intervals after the last feeding, 400IU eCG was injected at 11:00 on day 21; the interval is 80 hours; injection of 200IU hCG at 19:00 on day 24; the 19:00 pigs on day 24 began oestrous.

And (3) synchronous estrus detection: the operation laparotomy examination of the induced pig in this example shows that the ovaries are ovulated and red bodies appear, and as shown in fig. 2, the reproductive system of the pig develops well without any lesion. Wherein both ovaries are well developed and ovulated, and have appeared as red bodies, and the number of red bodies in the left side (shown as A in FIG. 2) and the number of red bodies in the right side (shown as B in FIG. 2) of the pig is 5.

Artificial insemination: artificial insemination is respectively carried out twice at 19:00 of 25 th day and 11:00 of 26 th day, and heat check is carried out twice every day, if no heat return exists within 21 days after insemination, the treated pigs are successfully fertilized, and enter a gestation period, and all the pigs are successfully fertilized after heat check.

Example 3. method of inducing estrus in replacement gilts.

Selecting 18 replacement sows which are healthy and have no disease, particularly can not carry reproduction-related diseases, are aged for 220 days, have the weight of 140kg and are at medium fat condition.

After selecting pigs, feeding 20mg of altrenogest for 20 days at a specified time, such as 14:00 pm each day; at an interval of 42 hours after the last feeding, 400IU eCG was injected at 6:00 on day 22; the interval is 80 hours; injection of 200IU hCG at 14:00 on day 25; the 14:00 pigs on day 26 began oestrous.

And (3) synchronous estrus detection: the induced pigs in this example were subjected to an operation laparotomy examination to find that all ovaries had ovulated and appeared as red bodies, as shown in fig. 3, the pig was 320, the reproductive system had developed well, and no lesion appeared. The ovaries on both sides developed well and had ovulated and appeared as red bodies, and the number of red bodies in the left side (shown as A in FIG. 3) and the number of red bodies in the right side (shown as B in FIG. 3) of the pig was 7.

Artificial insemination: artificial insemination is respectively carried out twice at 14:00 of 25 th day and 5:00 of 26 th day, and heat check is carried out twice every day, if no heat return exists in 21 days, the treated pigs are successfully fertilized, and enter the gestation period, and all the pigs are successfully fertilized through heat check.

Claims

1. A method for inducing replacement sows to estre synchronously is characterized in that each pig is fed with altrenogest at a fixed point every day, after the pigs are continuously fed for 18-20 days and are separated by 42-48 hours, each pig is injected with equine chorionic gonadotropin (eCG), and after the pigs are separated by 80-85 hours, each pig is injected with human chorionic gonadotropin (hCG), after the pigs are separated by 24 hours, the pigs are estrated, artificial insemination is performed on the sows, the artificial insemination frequency is two times, namely the 24h and the 40h after the pigs are estrated, the fixed point altrenogest amount fed every day by each pig is 16-20mg, the eCG injection amount by each pig is 400IU, and the hCG injection amount by each pig is 200 IU.

2. The method of inducing estrus in replacement gilts as in claim 1 wherein said pigs are continuously fed altrenogest for 18 days, 42 hours later, eCG is injected, and 80 hours later, hCG is injected.

3. The method of inducing estrus in replacement gilts as in claim 1 or 2 wherein the replacement gilts are 220-230 days old and 130-140 kg in weight.

4. The method of inducing estrus in replacement gilts as in claim 1 or 2 wherein the ovaries of said estrous pigs have ovulated and red bodies appear.

5. The method of inducing estrus in replacement gilts as in claim 1 or 2 wherein artificial insemination is performed after the oestrus of the pigs, no estrus return occurs within 21 days, and the treated pigs are pregnant and enter gestation.