CN108841767B - A kind of complex micro organism fungicide and microbial-bacterial fertilizer - Google Patents
A kind of complex micro organism fungicide and microbial-bacterial fertilizer Download PDFInfo
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Abstract
The present invention provides a kind of complex micro organism fungicide and microbial-bacterial fertilizer, is related to microbial-bacterial fertilizer field.The complex micro organism fungicide contains bacillus subtilis XF-1, trichoderma viride, Bifidobacterium, saccharomycete, lactic acid bacteria, nitrobacteria, nitrogen-fixing bacteria and EM bacterium.The microbial-bacterial fertilizer is prepared with the following method: the complex micro organism fungicide as claimed in claim 1 or 2 of fermentation bed matrix weight 0.5-5% being added in fermentation bed substrate, obtains fermentation bed carrier；The fermentation bed carrier is laid in pigsty or cattle pen, into pig or cattle breeding 50-70 days, obtains fermentation bed；The fermentation bed pH to 6.8-7.8 is adjusted, then adjusting water content is 55-65%, compost, drying obtain the microbial-bacterial fertilizer.Rationally, after fermenting in fermentation bed substrate, microbial-bacterial fertilizer, which is prepared, can significantly improve the yield and quality of crop for microbe species collocation in complex micro organism fungicide of the present invention.
Description
Technical field
The present invention relates to microbial-bacterial fertilizer fields, more particularly to a kind of complex micro organism fungicide and microbial bacteria
Fertilizer.
Background technique
Since the modernization level of agricultural is higher and higher, green non-pollution food is increasingly valued by people.Therewith
What is paid attention to is the ecological agriculture.Because only that the agricultural of ecology can just produce the food of ecology.In this regard, either from
In the dynamics that national policy is supported, or in actual action, country all puts into a large amount of support to this.Now we institute
The extensive use of the fertilizer containing active microorganism of solution is exactly a best explanation.It can be said that the fertilizer containing microorganism with
The indispensable fertilizer that will be increasingly becoming in crop growth afterwards.
Microbial-bacterial fertilizer not only contains organic matter, but also living microorganisms and its metabolin containing specific function, is applied to
Agricultural production increases the supply of plant nutrient or promotes to plant by the vital movement and metabolite of microorganism contained therein
Object growth, improves yield, improves quality of agricultural product and agroecological environment；The mechanism of action mainly passes through the powerful breeding of viable bacteria
Ability and competitiveness inhibit germ, activating soil, be on the other hand it is antibacterial by the metabolite of viable bacteria, desinsection, promote to make
Object growth, gives back soil health.
Microbial-bacterial fertilizer in the prior art can't be satisfactory in terms of diseases prevention and volume increase.
Summary of the invention
The main object of the present invention is to provide a kind of complex micro organism fungicide, the microbial inoculum contain bacillus subtilis XF-1,
Trichoderma viride, Bifidobacterium, saccharomycete, lactic acid bacteria, nitrobacteria, nitrogen-fixing bacteria and EM bacterium are arranged in pairs or groups due to microbe species and are closed
Excellent microbial-bacterial fertilizer can be prepared after fermenting in fermentation bed substrate in reason.
It is a further object to provide the microbial-bacterial fertilizer using the preparation of above-mentioned complex micro organism fungicide, micro- lifes
Object bacterial manure has the advantages of organic fertilizer, microorganism live bacteria preparation concurrently, can improve utilization rate of fertilizer, increases the soil organism, promotes to make
The breeding of object rhizosphere microorganism, improves the physicochemical property and bioactivity of soil, provides comprehensive, balanced battalion for the growth of crop
It supports, and can prevent and reduce the generation of soil-borne disease, the yield and quality of crop is made to be significantly improved.
The purpose of the present invention adopts the following technical scheme that realization.
A kind of complex micro organism fungicide contains bacillus subtilis (Bacillus subtilis) XF-1, Trichoderma viride
Bacterium, Bifidobacterium, saccharomycete, lactic acid bacteria, nitrobacteria, nitrogen-fixing bacteria and EM bacterium.
In preferred technical solution, the complex micro organism fungicide is grouped as by the group of following parts by weight: content is not less than
10×108Bacillus subtilis XF-1 microbial inoculum 10-30 parts by weight, the content of cfu/g is not less than 1 × 108The Trichoderma viride of cfu/g
Bacteria agent 5-20 parts by weight, content are not less than 10 × 108The Bifidobacterium microbial inoculum 2-10 parts by weight of cfu/g, content not less than 2 ×
108Yeast bacteria agent 5-10 parts by weight, the content of cfu/g is not less than 2 × 108The lactic acid bacteria agent 5-10 parts by weight of cfu/g contain
Amount is not less than 1 × 108Nitrifying bacterial agent 2-10 parts by weight, the content of cfu/g is not less than 1 × 108The fixed nitrogen bacteria agent of cfu/g
2-10 parts by weight, content are not less than 5 × 108The EM bacteria agent 5-20 parts by weight of cfu/g.Wherein, vinasse are after wine brewing is remaining
Solid content, sugar residue are the discarded solid contents extracted after sugar, and acid-sludge is the discarded solid content after vinegar processed, and bagasse is sugarcane in sugar refinery
The remaining substance after squeezing sugar, solid during soya-bean oil slag is the cold filtering technology when squeezing process produces soya-bean oil are remaining
Object, marc are remaining solid residues after squeezed juice, and manioc waste is that cassava extracts remaining solid residue after starch, edible mushroom
Slag is remaining discarded solid content after various mushroom cultures, and weeds are cut into the segment no more than 10cm, and corn stover, which is cut into, not to be surpassed
Cross the segment of 10cm.The material selection of bed substrate Central Plains ferment in line with the principle for being easy to get, gathering materials on the spot.
The microbial-bacterial fertilizer using complex micro organism fungicide preparation is also claimed in the present invention.
In the present invention, the microbial-bacterial fertilizer is prepared with the following method: fermentation bed base is added in fermentation bed substrate
The complex micro organism fungicide as claimed in claim 1 or 2 of matter weight 0.5-5%, obtains fermentation bed carrier；By the fermentation bed carrier
It is laid in pigsty or cattle pen, into pig or cattle breeding 50-70 days, obtains fermentation bed；The fermentation bed pH to 6.8-7.8 is adjusted,
Adjusting water content again is 55-65%, and compost, drying obtain the microbial-bacterial fertilizer.The cultivation fecaluria of traditional pig and ox pollutes
It is very serious, serious influence is produced on ground, air, water source.Fermentation bed carrier and excrement of animals cooperative fermentation, can be fast
Speed converts the breeding wastes such as raw excrement, urine, eliminates stench, inhibits pest, germ, meanwhile, it is beneficial in complex micro organism fungicide
Microbial bacteria group energy will ferment bed substrate, excrement synthesis for the battalion such as the carbohydrate of livestock edible, protein, organic acid, vitamin
Substance is supported, livestock disease resistance is enhanced, promotes animal health growth.Due to the microbial-bacterial fertilizer (withered grass bud containing a large amount of beneficial bacteriums
Spore bacillus XF-1, trichoderma viride, Bifidobacterium, saccharomycete, lactic acid bacteria, nitrobacteria, nitrogen-fixing bacteria, EM bacterium etc.) and active matter
Matter (organized enzyme, protease, phytokinase, organic acid, amino acid, a variety of middle microelements), therefore can activating soil, enhancement soil
Earth fertility, the formation for promoting soil aggregate structure, alkali resistant solution salt improve soil, promote absorption of the crop to nutrient, stimulate crop
Root growth.Enhance plant disease-resistant (worm) and drought-resistant ability.Multiple beneficial microorganism forms advantage in crop rhizosphere mass propagation
Flora, and plant growth-promoting substance and antibiotic are secreted, inhibit the growth of pathogenic microorganism, improves resistance to bacterial wilt, clubroot, root-rot
The ability of the multiple diseases such as disease, damping-off, epidemic disease, wilt disease, root knot nematode disease.Fermentation bed carrier thickness at least 30cm.
In the present invention, the fermentation bed substrate is selection rice husk, peanut shell, cotton seed hulls, vinasse, sugar residue, acid-sludge, sugarcane
One or more of slag, sawdust, the dregs of fat, marc, leaf, weeds, stalk, edible fungi residues, manioc waste substance mixes
Close object.
In preferred technical solution, fermentation bed pH is adjusted using lime or calcium superphosphate.
In preferred technical solution, in composting process, when temperature is more than 50 DEG C in fermentation bed, the fermentation bed is carried out
It stirs.In this application, the compost time is 7-10 days.
In preferred technical solution, the water content of the microbial-bacterial fertilizer is not higher than 30%, viable bacteria content not less than 5 ×
107Cfu/g, pH 6.5-8.5.
Complex micro organism fungicide of the present invention contain bacillus subtilis XF-1, trichoderma viride, Bifidobacterium, saccharomycete,
Lactic acid bacteria, nitrobacteria, nitrogen-fixing bacteria and EM bacterium, in the complex micro organism fungicide microbe species collocation rationally, in fermentation bed base
After fermenting in matter, excellent microbial-bacterial fertilizer can be prepared.Microbial-bacterial fertilizer of the present invention has organic fertilizer, microorganism live bacteria concurrently
The advantages of preparation, can improve utilization rate of fertilizer, increase the soil organism, promote rhizosphere microorganisms of crops breeding, improve soil
Physicochemical property and bioactivity provide comprehensive, balanced nutrition for the growth of crop, and can prevent and reduce the hair of soil-borne disease
It is raw, so that the yield and quality of crop is significantly improved.
Specific embodiment
The present invention is further illustrated with reference to embodiments.
The preparation of 1 complex micro organism fungicide of embodiment
Present embodiment describes the preparation methods that complex micro organism fungicide to be used is needed in microbial-bacterial fertilizer preparation process.
(1) preparation of bacillus subtilis (Bacillus subtilis) XF-1 microbial inoculum
The work of bacillus subtilis (Bacillus subtilis) XF-1 (hereafter abbreviated with bacillus subtilis XF-1)
Change: from the inclined-plane of bacillus subtilis XF-1, with aseptic inoculation needle picking part lawn into LB shaking flask (in 250mL shaking flask
LB liquid medium liquid amount 50ml), after cultivating for 24 hours at 30 DEG C, 150r/min, LB culture medium is become cloudy.Take bacterium solution to LB
- 36h for 24 hours is cultivated in scribing line in solid medium, and bacillus subtilis XF-1 is good in LB cultured on solid medium, bacteria colony white
Or canescence, edge is round and smooth, surface imperfection, opaque.
The preparation of seed liquor: the liquid amount of LB liquid culture medium is 150ml in 500mL seed flask.The training of picking LB solid
The single colonie supported on base is inoculated in seed flask, and seed flask is cultivated for 24 hours at 30 DEG C, 150r/min, obtains seed liquor.
Fermentative medium formula (g/L) are as follows: groundnut meal 15g/L, cornstarch 20g/L, glucose 10g/L, CaCO3
2g/L, (NH4)2SO40.5g/L, MgSO4·7H2O 0.2g/L, GPE 0.1g/L, pH 7.0-7.5.
Fermentation medium is placed in fermentor, is sterilized.
It by the seed liquor of bacillus subtilis XF-1, accesses in fermentor, condition of culture is as follows: ventilation ratio 1:1, stirring
Revolving speed is 100-150r/min, tank presses 0.01MPa, 30 DEG C of cultivation temperature, incubation time 48h, obtain viable bacteria content be 10 ×
108The fermentation liquid of cfu/mL, wherein spore content is 95%.The fermentation liquid can be directly as liquid microbial inoculum.
At the end of fermentation tank culture, guarantee that viable bacteria content is 5-1000 × 10 in liquid microbial inoculum8Cfu/mL and therein
Under the premise of gemma rate is not less than 95%, fermentation medium and fermentation culture conditions can be adjusted.
In the case where obtaining above-mentioned bacillus subtilis XF-1 liquid microbial inoculum, the solid of bacillus subtilis XF-1 is prepared
Preparation.
To liquid microbial inoculum obtained, (viable bacteria content is 10 × 108Cfu/mL and wherein gemma rate are 95%) middle addition 1000
The weight ratio of purpose diatomite chamotte powder, liquid microbial inoculum and diatomite chamotte powder is 100:20.The liquid-solid mixture is stirred 30
After minute, feed liquid is inputted into drying in atomizer tower using dehvery pump, obtaining water content is 5.6% (W/W), viable bacteria content
It is 48 × 108The bacillus subtilis XF-1 solid-state microbial inoculum I of cfu/g.The wherein drying condition in atomizer tower are as follows: air inlet
Mouth temperature is 180-200 DEG C, and air outlet temperature is 75-85 DEG C.
Liquid microbial inoculum can also first use ceramic membrane thickening filtration, and concentrate weight 3% is added in obtained concentrate
The diatomite chamotte powder of 1000 mesh is dried in vacuo under the conditions of 80 DEG C after mixing, and obtaining water content is 6% (W/
W), viable bacteria content is 115 × 108The solid fungicide II of cfu/g.
It is 20 × 10 to prepare bacillus subtilis XF-1 content8The solid-state microbial inoculum of cfu/g, can be in solid fungicide I
Or in II, the neopelex of its quality 5% (W/W) and the diatomite chamotte powder of appropriate 1000 mesh are added, mixing is equal
Air-flow crushing after even.
(2) preparation of composite bacteria agent:
The liquid microbial inoculum or solid-state microbial inoculum for taking the above-mentioned bacillus subtilis XF-1 prepared, with other microbial bacterial agents
It is uniformly mixed according to following ratio: bacillus subtilis XF-1 microbial inoculum (self-control, content 20 × 108Cfu/g) 10-30 parts by weight,
(Yunnan Xing Yao biological products Co., Ltd, content are 1 × 10 to Trichoderma viride bacteria agent8Cfu/g) 5-20 parts by weight, Bifidobacterium
(Shaanxi Sen Fu natural product Co., Ltd, content are 10 × 10 to microbial inoculum8Cfu/g) 2-10 parts by weight, (Shanghai is green for yeast bacteria agent
Lai Qing Biotechnology Co., Ltd, content are 2 × 108Cfu/g) 5-10 parts by weight, lactic acid bacteria agent (the gloomy not natural product in Shaanxi
Co., Ltd, content are 2 × 108Cfu/g) (Fujian running water Bioisystech Co., Ltd goes out for 5-10 parts by weight, nitrifying bacterial agent
Product, content are 1 × 108Cfu/g) 2-10 parts by weight, fixed nitrogen bacteria agent (Hua Long Biotechnology Co., Ltd, Heilongjiang Province, content
It is 1 × 108Cfu/g) (Cangzhou City Yi Hong Biotechnology Co., Ltd, content are 5 × 10 for 2-10 parts by weight, EM bacteria agent8cfu/
G) 5-20 parts by weight obtain complex micro organism fungicide.
Preparation containing following component complex micro organism fungicide A: bacillus subtilis XF-1 microbial inoculum (content be 20 ×
108Cfu/g) (content is 1 × 10 for 20 parts by weight, Trichoderma viride bacteria agent8Cfu/g) 10 parts by weight, Bifidobacterium microbial inoculum (content
It is 10 × 108Cfu/g) (content is 2 × 10 for 8 parts by weight, yeast bacteria agent8Cfu/g) 8 parts by weight, lactic acid bacteria agent (content 2
×108Cfu/g) (content is 1 × 10 for 6 parts by weight, nitrifying bacterial agent8Cfu/g) 6 parts by weight, fixed nitrogen bacteria agent (content be 1 ×
108Cfu/g) (content is 5 × 10 for 8 parts by weight, EM bacteria agent8Cfu/g) 15 parts by weight.
Preparation contains the control microbial inoculum 1 of following component: (content is 20 × 10 to bacillus subtilis XF-1 microbial inoculum8cfu/g)
(content is 1 × 10 for 20 parts by weight, Trichoderma viride bacteria agent8Cfu/g) (content is 5 × 10 for 10 parts by weight, EM bacteria agent8cfu/g)
15 parts by weight.
Preparation contains the control microbial inoculum 2 of following component: (content is 10 × 10 to Bifidobacterium microbial inoculum8Cfu/g) 8 parts by weight,
(content is 2 × 10 to yeast bacteria agent8Cfu/g) (content is 5 × 10 for 8 parts by weight, EM bacteria agent8Cfu/g) 15 parts by weight.
Preparation contains the control microbial inoculum 3 of following component: (content is 2 × 10 to lactic acid bacteria agent8Cfu/g) 6 parts by weight, nitrification
(content is 1 × 10 to bacterium microbial inoculum8Cfu/g) (content is 1 × 10 for 6 parts by weight, fixed nitrogen bacteria agent8Cfu/g) 8 parts by weight, EM bacterium bacterium
(content is 5 × 10 for agent8Cfu/g) 15 parts by weight.
The preparation of 2 microbial-bacterial fertilizer of embodiment
Microbial-bacterial fertilizer is prepared using complex micro organism fungicide A, is included the following steps:
Step 1: fermenting in pigsty, the fermentation bed rich in beneficial microbe, organic matter and a large amount of active materials is obtained.
Take 500 parts by weight of rice husk, 200 parts by weight of brewex's grains, 100 parts by weight of sawdust, 100 parts by weight of soya-bean oil slag, leaf 50
Parts by weight, 50 parts by weight of edible fungi residues, 50 parts by weight of weeds, 200 parts by weight of corn stover are uniformly mixed, and obtain fermentation bed base
Matter.Wherein brewex's grains are remaining solid contents after brewing, and soya-bean oil slag is the cold filtering technology mistake when squeezing process produces soya-bean oil
Solid residue in journey, edible fungi residues are remaining discarded solid contents after various mushroom cultures.
The complex micro organism fungicide A (preparation of embodiment 1) that its weight 1% is accessed in fermentation bed substrate, is uniformly mixed, spray
The water content that water adjusts fermentation bed substrate reaches 20%, obtains fermentation bed carrier.
Fermentation bed carrier is laid in pigsty or cowshed, fermentation bed carrier thickness is that 50cm was obtained into swine rearing 2 months
To fermentation bed.Single pigsty area takes up an area 1.5 square metres of density culture according to every pig between 20-50 square meter.When containing
After the excrement and urine of the fermentation bed carrier adsorption of complex micro organism fungicide A pig, then repeatedly, chronically trample on through pig, stir,
It mixes and carries out spontaneous fermentation and part is decomposed, per two weeks is manually or mechanically thoroughly stirred once, it is ensured that be uniformly mixed up and down.
If excrement is unevenly distributed on fermentation bed carrier, then thin soil pipe reason is carried out within every 2-3 days once, it is ensured that excrement is evenly distributed on fermentation
On bed carrier.Fermentation bed carrier can be lost, while absorption, digestion, decomposing excrement when loss reaches 10% or more
When, new fermentation bed carrier is replenished in time, new fermentation bed carrier will be uniformly mixed with old fermentation bed carrier.
Into after swine rearing 2 months, obtain adsorbing fermentation abundant, uniformly mixed, full of nutrition, that beneficial microbe content is high
Bed.It is thin that the fermentation bed not only contains bacillus subtilis XF-1, trichoderma viride, Bifidobacterium, saccharomycete, lactic acid bacteria, nitrification
The beneficial microbes such as bacterium, nitrogen-fixing bacteria, EM bacterium, and containing organized enzyme, phytokinase, carbohydrate, protein, organic acid, amino acid,
The necessary substance of the crop growths such as vitamin, microelement, N, P, K.
Can also be into cattle breeding, according to the size of ox generally according to following density culture: the ox land occupation 2 of 100kg or so be flat
The calf of square rice/head, the 400kg or so that grows up takes up an area 4-5 square metres/head, and big 6-7 square metres of ox land occupation of growing up and (be greater than 500kg)/
Head.
Step 2: there are also the incomplete decomposed organic matter in part and some diseases in the fermentation bed obtained due to the first step
Bacterium, worm's ovum and weed seed, therefore germ, worm's ovum and weed seed are killed by compost.
The specific method is as follows for compost: making fermentation bed water content 60% by spray water in fermentation bed, in this way can
Promote microbial activities and compost fermentation.Appropriate lime or calcium superphosphate is added, adjusts pH to 6.8-7.8.Material is piled into cross
Section is that trapezoidal strip carries out compost, and wide 1.5~2.0 meters or so of bottom, high 80~100cm or so, length is unlimited, when compost
Between be 8 days.It is stirred when temperature is more than 50 DEG C in fertilizer heap primary.In composting process, keeps having air appropriate in compost, have
The breeding and activity of sharp aerobe promote organic matter to decompose.
Step 3: through second step treated fermentation bed, dry, sieving obtains microbial-bacterial fertilizer A.
It is dry using the method for drying, cylinder drying, boiled bed drying method or bipyramid rotary through second step treated fermentation bed
Dry method dry (drying temperature is not higher than 85 DEG C) is 25% to water content, crosses the vibrating screen that aperture is 2mm after mixing, obtains
Microbial-bacterial fertilizer A.
Microbial-bacterial fertilizer A is taken, " composite microbiological fertilizer executes standard (NY/ according to The Ministry of Agriculture of the People's Republic of China, MOA
T798-2015) " in method measure all data, and in execution standard each index compare.As a result it is shown in Table 1 and table respectively
2。
1 microbial-bacterial fertilizer determination data of table
| Project | Index request in NY/T798 | Detection data |
| Effective viable bacteria content | ≥0.2×108cfu/g | 9.6×108cfu/g |
| Total nutrient (N+P2O5+K2O), % | 8.0~25.0 | 15.7 |
| Organic matter (to dry in terms of base), % | ≥20 | 25.8 |
| Water content, % | ≤30 | 25.0 |
| pH | 5.5~8.5 | 7.23 |
The measurement of 2 microbial-bacterial fertilizer harmful substance contents of table
| Project | Limit index in NY/T798 | Detection data |
| Excrement colibacillus group number, a/g | ≤100 | 57 |
| Induced worm egg death rate, % | ≥95 | 98.7 |
| Arsenic (As) (to dry in terms of base), mg/kg | ≤15 | 7.8 |
| Cadmium (Cd) (to dry in terms of base), mg/kg | ≤3 | 1.3 |
| Lead (Pb) (to dry in terms of base), mg/kg | ≤50 | 13.7 |
| Chromium (Cr) (to dry in terms of base), mg/kg | ≤150 | 25.9 |
| Mercury (Hg) (to dry in terms of base), mg/kg | ≤2 | 0.54 |
By the data of Tables 1 and 2 it is found that the microbial-bacterial fertilizer A that the present embodiment obtains, indices are attained by agricultural
Requirement in portion's " composite microbiological fertilizer executes standard (NY/T798-2015) ".
Control 1,2,3 alternative composite microbial bacterial agent A of microbial inoculum is respectively adopted and prepares microbial-bacterial fertilizer, obtains control microorganism
Bacterial manure 1,2,3.Each control microbial-bacterial fertilizer meets The Ministry of Agriculture of the People's Republic of China, MOA, and " composite microbiological fertilizer executes standard
(NY/T798-2015) " in indices.
The field efficacy of 3 microbial-bacterial fertilizer of embodiment
Using the microbial-bacterial fertilizer prepared in embodiment 2, a large amount of test is carried out in Yunnan Province.
Apply crop and prevention/controlling disease: bacterial wilt of tomato, cabbage clubroot, root rotof flax, cucumber rhizoctonia rot,
Capsicum epidemic disease, watermelon blight, groundnut root knot nematode disease.
With fertile scheme:
It chooses six to be compared with fertile scheme: CK (not applying fertilizer), commercially available general organic fertilizer (General
Organic Fertilizer, convenient for label, writes a Chinese character in simplified form are as follows: GOF), microbial-bacterial fertilizer A, control microbial-bacterial fertilizer 1 (be abbreviated as
D1), microbial-bacterial fertilizer 2 (being abbreviated as D2), control microbial-bacterial fertilizer 3 (being abbreviated as D3) are compareed.Microbial-bacterial fertilizer A, D1, D2, D3
It is prepared by method in embodiment 2.
Suggested use and dosage: applying fertilizer 3 times in every kind of plant cultivating process, is used as base manure for the first time, second, third time is to chase after
Fertilizer, fertilising is applied according to 150kg/ mus of standard every time.
Experimental plot setting: 18 cells are arranged at 20-30 square metres or so in single subdistrict area, and each experiment is 3 small
Area can set isolation strip in the big place of field area.
It only applies fertilizer different, other management and nursery etc. are just the same.Growing state, disease index etc. are counted respectively, often
After 3 repetitions of a processing are averaged, the using effect of each fertilizer fertilising is calculated.Its test data is shown in Table 3-tables 9 respectively.
Application effect of 3 microbial-bacterial fertilizer of table on tomato
Application effect of 4 microbial-bacterial fertilizer of table on Chinese cabbage
Application effect of 5 microbial-bacterial fertilizer of table on wheat
Application effect of 6 microbial-bacterial fertilizer of table on cucumber
Application effect of 7 microbial-bacterial fertilizer of table on capsicum
Application effect of 8 microbial-bacterial fertilizer of table on watermelon
Application effect of 9 microbial-bacterial fertilizer of table on peanut
By the data of above-mentioned 3-table of table 9 (the wherein processing that A indicates application microbial-bacterial fertilizer A) it is found that microbial-bacterial fertilizer A
Not only to tomato, Chinese cabbage, wheat, cucumber, capsicum, watermelon, peanut etc. have apparent growth promoting function, but also to bacterial wilt,
The diseases such as clubroot, root rot, damping-off, epidemic disease, wilt disease, root knot nematode disease also have apparent prevention and treatment or preventive effect.
Therefore, this hair microbial-bacterial fertilizer can not only improve absorption, promotion plant growth of the crop to nutrient.And plant can be enhanced
Disease resistance.By site competition, secretion plant growth-promoting substance and antibiotic, inhibits the growth of pathogenic microorganism, improve anti-blueness
The ability of the multiple diseases such as blight, clubroot, root rot, damping-off, epidemic disease, wilt disease, root knot nematode disease.
Claims (7)
1. using the microbial-bacterial fertilizer of complex micro organism fungicide preparation, it is characterised in that the complex micro organism fungicide contains withered grass
Bacillus XF-1, trichoderma viride, Bifidobacterium, saccharomycete, lactic acid bacteria, nitrobacteria, nitrogen-fixing bacteria and EM bacterium；Micro- life
Object bacterial manure is prepared with the following method: the complex microorganism of fermentation bed matrix weight 0.5-5% being added in fermentation bed substrate
Microbial inoculum obtains fermentation bed carrier；The fermentation bed carrier is laid in pigsty or cattle pen, into pig or cattle breeding 50-70 days, is obtained
To fermentation bed；The fermentation bed pH to 6.8-7.8 is adjusted, then adjusting water content is 55-65%, compost, drying obtain described micro-
Bio-bacterial manure.
2. microbial-bacterial fertilizer according to claim 1, it is characterised in that the complex micro organism fungicide is by following parts by weight
Group is grouped as: content is not less than 10 × 108Bacillus subtilis (Bacillus subtilis) the XF-1 microbial inoculum 10- of cfu/g
30 parts by weight, content are not less than 1 × 108Trichoderma viride bacteria agent 5-20 parts by weight, the content of cfu/g is not less than 10 × 108cfu/
Bifidobacterium microbial inoculum 2-10 parts by weight, the content of g is not less than 2 × 108The yeast bacteria agent 5-10 parts by weight of cfu/g, content are not
Lower than 2 × 108Lactic acid bacteria agent 5-10 parts by weight, the content of cfu/g is not less than 1 × 108The nitrifying bacterial agent 2-10 of cfu/g
Parts by weight, content are not less than 1 × 108Fixed nitrogen bacteria agent 2-10 parts by weight, the content of cfu/g is not less than 5 × 108The EM bacterium of cfu/g
Microbial inoculum 5-20 parts by weight.
3. microbial-bacterial fertilizer according to claim 1 or claim 2, it is characterised in that the fermentation bed substrate is selection rice husk, peanut
Shell, cotton seed hulls, vinasse, sugar residue, acid-sludge, bagasse, sawdust, the dregs of fat, marc, leaf, weeds, stalk, edible fungi residues, cassava
The mixture of one or more of slag substance.
4. microbial-bacterial fertilizer according to claim 3, it is characterised in that adjust fermentation bed pH using lime or calcium superphosphate.
5. microbial-bacterial fertilizer according to claim 4, it is characterised in that in composting process, when temperature is more than 50 DEG C in fermentation bed
When, the fermentation bed is stirred.
6. microbial-bacterial fertilizer according to claim 5, it is characterised in that the compost time is 7-10 days.
7. microbial-bacterial fertilizer according to claim 6, it is characterised in that the water content of the microbial-bacterial fertilizer is not higher than 30%,
Viable bacteria content is not less than 5 × 107Cfu/g, pH 6.5-8.5.
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| CN109651016B (en) * | 2018-12-28 | 2021-11-09 | 深圳合民生物科技有限公司 | Microbial organic fertilizer and preparation method thereof |
| CN111777473A (en) * | 2020-08-14 | 2020-10-16 | 贵州大学 | Slow-release fertilizer for improving soil activity of roxburgh rose garden and preparation method thereof |
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| CN113307678B (en) * | 2021-04-25 | 2023-08-15 | 云南星耀生物制品有限公司 | Composite microbial fertilizer and field application thereof |
| CN114455987A (en) * | 2022-01-25 | 2022-05-10 | 广西大学 | Beef cattle manure and urine integrated bio-organic fertilizer and preparation method thereof |
| CN115353418A (en) * | 2022-08-17 | 2022-11-18 | 云南星耀生物制品有限公司 | Composite microbial fertilizer |
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