CN108823096A - A kind of biotechnology cell culture apparatus - Google Patents
A kind of biotechnology cell culture apparatus Download PDFInfo
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- CN108823096A CN108823096A CN201810922126.2A CN201810922126A CN108823096A CN 108823096 A CN108823096 A CN 108823096A CN 201810922126 A CN201810922126 A CN 201810922126A CN 108823096 A CN108823096 A CN 108823096A
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- Prior art keywords
- incubator
- fixedly connected
- cell culture
- partition
- culture apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of biotechnology cell culture apparatus, including incubator, the lower end of the incubator is fixedly connected with multiple stabilizer blades, the incubator is equipped with device chamber, described device is intracavitary to be equipped with mobile device, mounting plate is fixedly connected in the mobile device, the lower end of the mounting plate is fixedly connected with humidifier, the side wall of the incubator is equipped with gas supply mechanism, the side wall of the incubator, which runs through, is equipped with oxygen detection instrument, the inner wall of the incubator is fixedly connected with first partition, is equipped with second partition in the incubator.Structure of the invention design rationally, can prevent the cross-infection between cell, improve the quality of cell, and can uniformly carry out increase humidity.
Description
Technical field
The present invention relates to culture apparatus technical field more particularly to a kind of biotechnology cell culture apparatus.
Background technique
For entire biotechnology, cell culture is all an essential process, cell culture itself
Be exactly the extensive clone of cell, cell culture technology can by a cell by mass propgation become it is simple unicellular or
The many cells seldom broken up, in order to be utilized, it may be said that cell culture technology is most crucial, most basic in biotechnology
Technology.
It generally requires to use cell incubator in biological cell incubation, and traditional cell is in the mistake of culture
Cheng Zhong is located in a biggish inner cavity, is not had interlayer, is easily lead to the cross-infection between cell in this way,
The cell quality turned out is not high, and cell incubator does not have the function of humidification.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and a kind of biotechnology cell training proposed
Device is supported, the cross-infection between cell can be prevented, improve the quality of cell, and can uniformly carry out increase humidity.
To achieve the goals above, present invention employs following technical solutions：
A kind of biotechnology cell culture apparatus, including incubator, the lower end of the incubator are fixedly connected with multiple
Foot, the incubator are equipped with device chamber, and described device is intracavitary to be equipped with mobile device, are fixedly connected with peace in the mobile device
Loading board, the lower end of the mounting plate are fixedly connected with humidifier, and the side wall of the incubator is equipped with gas supply mechanism, the incubator
Side wall run through and be equipped with oxygen detection instrument, the inner wall of the incubator is fixedly connected with first partition, is equipped in the incubator
Second partition, the both ends of the second partition are respectively fixedly connected on the lower end of first partition and the interior bottom of incubator.
Preferably, the mobile device includes the motor for being fixedly connected on incubator upper end, and the output shaft of the motor passes through
It wears the upper end of incubator and is fixedly connected with second bevel gear, the inner wall of described device chamber is rotatably connected to connecting rod, the company
Extension bar is fixedly connected with threaded rod far from one end of device cavity wall, and the threaded rod is rotatably connected on far from one end of connecting rod
On the inner wall of device chamber, first bevel gear, the first bevel gear and second bevel gear phase are fixedly connected in the connecting rod
It engages, is threaded with nut on the threaded rod, the lower end of the nut is fixedly connected with slide plate, and the lower end of the slide plate is passed through
The interior bottom of penetrating device chamber, the slide plate are slidably connected with incubator, and the mounting plate is fixedly connected on the lower end of slide plate.
Preferably, the gas supply mechanism includes the oxygen cylinder being fixedly connected on incubator side wall, is connected on the oxygen cylinder
It is connected to connecting tube, the connecting tube is connected on the side wall of incubator far from one end of oxygen cylinder, and the inner wall of the connecting tube is solid
Surely it is connected with valve.
Preferably, the connecting tube and incubator junction encapsulation process.
Preferably, the oxygen detection instrument and valve are electrically connected.
Preferably, the first partition is equipped with multiple through-holes.
Beneficial effects of the present invention：
1, when oxygen detection instrument shows the deficiency of oxygen in incubator, valve is opened, carries out oxygen by connecting tube
In incubator, oxygen concentration is made to restore normal；
2, start motor, the output shaft rotation of motor drives second bevel gear rotation, and second bevel gear rotation drives first
Bevel gear rotation, first bevel gear rotation drive connecting rod rotation, and connecting rod rotation drives threaded rod rotation, and threaded rod rotation makes
Nut is mobile, and nut is mobile to drive slide plate mobile, and slide plate is mobile to drive mounting plate mobile, and then can be mobile to humidifier, makes
It can uniformly carry out increase humidity；
3, by setting first partition and second partition, can be improved to avoid the cross-infection generated between internal cell
The quality of cell culture.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of biotechnology cell culture apparatus proposed by the present invention；
Fig. 2 is enlarged drawing at a kind of A of biotechnology cell culture apparatus proposed by the present invention.
In figure：1 motor, 2 threaded rods, 3 device chambers, 4 nuts, 5 slide plates, 6 mounting plates, 7 humidifiers, 8 oxygen detection instrument, 9
Through-hole, 10 first partitions, 11 incubators, 12 stabilizer blades, 13 second partitions, 14 valves, 15 connecting tubes, 16 oxygen cylinders, 17 connecting rods,
18 first bevel gears, 19 second bevel gears.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Referring to Fig.1-2, a kind of biotechnology cell culture apparatus, including incubator 11, the lower end of incubator 11 is fixed to be connected
Multiple stabilizer blades 12 are connected to, incubator 11 is equipped with device chamber 3, mobile device is equipped in device chamber 3, is fixedly connected in mobile device
There is mounting plate 6, the lower end of mounting plate 6 is fixedly connected with humidifier 7, and the model SZ-J029 of humidifier 7 starts motor 1, electricity
The output shaft rotation of machine 1 drives second bevel gear 19 to rotate, and the rotation of second bevel gear 19 drives first bevel gear 18 to rotate, and first
The rotation of bevel gear 18 drives connecting rod 17 to rotate, and the rotation of connecting rod 17 drives threaded rod 2 to rotate, and the rotation of threaded rod 2 moves nut 4
Dynamic, nut 4 is mobile to drive slide plate 5 mobile, and slide plate 5 is mobile to drive mounting plate 6 mobile, and then can be mobile to humidifier 7, makes it
Increase humidity can be uniformly carried out, the side wall of incubator 11 is equipped with gas supply mechanism, when oxygen detection instrument 8 is shown in incubator 11
When the deficiency of oxygen, valve 14 is opened, oxygen is carried out in incubator 11 by connecting tube 15, oxygen concentration is made to restore normal,
The side wall of incubator 11, which runs through, is equipped with oxygen detection instrument 8, and the inner wall of the model Z-1100 of oxygen detection instrument 8, incubator 11 are solid
Surely it is connected with first partition 10, is equipped with second partition 13 in incubator 11, the both ends of second partition 13 are respectively fixedly connected with the
It, can be to avoid interior by setting first partition 10 and second partition 13 on the lower end of one partition 10 and the interior bottom of incubator 11
The cross-infection generated between portion's cell improves the quality of cell culture.
Mobile device includes the motor 1 for being fixedly connected on 11 upper end of incubator, and motor 1 is servo motor, servo motor
Model MSME 5AZG1, the output shaft of motor 1 run through the upper end of incubator 11 and are fixedly connected with second bevel gear 19, device
The inner wall of chamber 3 is rotatably connected to connecting rod 17, and connecting rod 17 is fixedly connected with threaded rod 2, spiral shell far from one end of 3 inner wall of device chamber
Rasp bar 2 is rotatably connected on the inner wall of device chamber 3 far from one end of connecting rod 17, and the first cone tooth is fixedly connected in connecting rod 17
Wheel 18, first bevel gear 18 is meshed with second bevel gear 19, and nut 4 is threaded on threaded rod 2, and the lower end of nut 4 is solid
Surely it is connected with slide plate 5, the interior bottom of device chamber 3 is run through in the lower end of slide plate 5, and slide plate 5 is slidably connected with incubator 11, mounting plate 6
It is fixedly connected on the lower end of slide plate 5, increase humidity can be uniformly carried out, avoid humidity inconsistent, gas supply mechanism includes fixing
The oxygen cylinder 16 being connected on 11 side wall of incubator is connected with connecting tube 15 on oxygen cylinder 16, and connecting tube 15 is far from oxygen cylinder 16
One end be connected on the side wall of incubator 11, the inner wall of connecting tube 15 is fixedly connected with valve 14, in order to incubator 11
Oxygen is provided, connecting tube 15 and 11 junction encapsulation process of incubator prevent extraneous gas infection cell, oxygen detection instrument 8 with
Valve 14 is electrically connected, can be timely to oxygen is provided in incubator 11, and first partition 10 is equipped with multiple through-holes 9, so as to
In increase humidity and oxygen.
In the present invention, when oxygen detection instrument 8 shows the deficiency of oxygen in incubator 11, valve 14 is opened, connection is passed through
Pipe 15 carries out oxygen in incubator 11, makes oxygen concentration restore normally to turn off valve 14, opens humidifier 7, can be to training
It supports and increases humidity in case 11, start motor 1, the output shaft rotation of motor 1 drives second bevel gear 19 to rotate, second bevel gear 19
Rotation drives first bevel gear 18 to rotate, and the rotation of first bevel gear 18 drives connecting rod 17 to rotate, and the rotation of connecting rod 17 drives spiral shell
Rasp bar 2 rotates, and the rotation of threaded rod 2 keeps nut 4 mobile, and nut 4 is mobile to drive slide plate 5 mobile, and slide plate 5 is mobile to drive mounting plate 6
It is mobile, and then can be mobile to humidifier 7, allow to uniformly carry out increase humidity.
More than, it is merely preferred embodiments of the present invention, but scope of protection of the present invention is not limited thereto, it is any
Those familiar with the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its invents
Design is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of biotechnology cell culture apparatus, including incubator (11), which is characterized in that the lower end of the incubator (11)
It is fixedly connected with multiple stabilizer blades (12), the incubator (11) is equipped with device chamber (3), and movement is equipped in described device chamber (3)
Device is fixedly connected with mounting plate (6) in the mobile device, and the lower end of the mounting plate (6) is fixedly connected with humidifier
(7), the side wall of the incubator (11) is equipped with gas supply mechanism, and the side wall of the incubator (11), which runs through, is equipped with oxygen detection instrument
(8), the inner wall of the incubator (11) is fixedly connected with first partition (10), and second partition is equipped in the incubator (11)
(13), the both ends of the second partition (13) are respectively fixedly connected with the lower end in first partition (10) and the interior bottom of incubator (11)
In portion.
2. a kind of biotechnology cell culture apparatus according to claim 1, which is characterized in that the mobile device includes
It is fixedly connected on the motor (1) of incubator (11) upper end, the output shaft of the motor (1) runs through the upper end of incubator (11) and consolidates
Surely it is connected with second bevel gear (19), the inner wall of described device chamber (3) is rotatably connected to connecting rod (17), the connecting rod (17)
One end far from device chamber (3) inner wall is fixedly connected with threaded rod (2), the one end of the threaded rod (2) far from connecting rod (17)
It is rotatably connected on the inner wall of device chamber (3), is fixedly connected with first bevel gear (18) on the connecting rod (17), described first
Bevel gear (18) is meshed with second bevel gear (19), is threaded with nut (4) on the threaded rod (2), the nut (4)
Lower end be fixedly connected with slide plate (5), the interior bottom of device chamber (3) is run through in the lower end of the slide plate (5), the slide plate (5) with
Incubator (11) is slidably connected, and the mounting plate (6) is fixedly connected on the lower end of slide plate (5).
3. a kind of biotechnology cell culture apparatus according to claim 1, which is characterized in that the gas supply mechanism includes
The oxygen cylinder (16) being fixedly connected on incubator (11) side wall is connected with connecting tube (15) on the oxygen cylinder (16), described
Connecting tube (15) is connected on the side wall of incubator (11) far from the one end of oxygen cylinder (16), and the inner wall of the connecting tube (15) is solid
Surely valve (14) are connected with.
4. a kind of biotechnology cell culture apparatus according to claim 1, which is characterized in that the connecting tube (15) with
Incubator (11) junction encapsulation process.
5. a kind of biotechnology cell culture apparatus according to claim 1, which is characterized in that the oxygen detection instrument
(8) it is electrically connected with valve (14).
6. a kind of biotechnology cell culture apparatus according to claim 1, which is characterized in that the first partition (10)
It is equipped with multiple through-holes (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810922126.2A CN108823096A (en) | 2018-08-14 | 2018-08-14 | A kind of biotechnology cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810922126.2A CN108823096A (en) | 2018-08-14 | 2018-08-14 | A kind of biotechnology cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108823096A true CN108823096A (en) | 2018-11-16 |
Family
ID=64153890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810922126.2A Pending CN108823096A (en) | 2018-08-14 | 2018-08-14 | A kind of biotechnology cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108823096A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112251347A (en) * | 2020-09-30 | 2021-01-22 | 福建中凯检测技术有限公司 | Biochemical culture apparatus for environmental detection |
| CN115772471A (en) * | 2023-02-13 | 2023-03-10 | 山东第一医科大学附属省立医院(山东省立医院) | Organ chip culture apparatus capable of adjusting oxygen concentration |
-
2018
- 2018-08-14 CN CN201810922126.2A patent/CN108823096A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112251347A (en) * | 2020-09-30 | 2021-01-22 | 福建中凯检测技术有限公司 | Biochemical culture apparatus for environmental detection |
| CN115772471A (en) * | 2023-02-13 | 2023-03-10 | 山东第一医科大学附属省立医院(山东省立医院) | Organ chip culture apparatus capable of adjusting oxygen concentration |
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Application publication date: 20181116 |
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