CN108822070A - One kind is for detecting homocysteine structure fluorescence probe para-compound and its application - Google Patents
One kind is for detecting homocysteine structure fluorescence probe para-compound and its application Download PDFInfo
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- CN108822070A CN108822070A CN201810606849.1A CN201810606849A CN108822070A CN 108822070 A CN108822070 A CN 108822070A CN 201810606849 A CN201810606849 A CN 201810606849A CN 108822070 A CN108822070 A CN 108822070A
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- Prior art keywords
- homocysteine
- compound
- fluorescence intensity
- application
- fluorescence probe
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- FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical group OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 title claims abstract description 37
- 239000000523 sample Substances 0.000 title claims abstract description 19
- 150000001875 compounds Chemical class 0.000 claims description 16
- 239000012895 dilution Substances 0.000 claims description 6
- 238000010790 dilution Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 235000001014 amino acid Nutrition 0.000 abstract description 5
- 150000001413 amino acids Chemical class 0.000 abstract description 5
- 238000001514 detection method Methods 0.000 abstract description 4
- 235000018417 cysteine Nutrition 0.000 abstract description 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 3
- 238000005935 nucleophilic addition reaction Methods 0.000 abstract description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 208000030613 peripheral artery disease Diseases 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/78—Ring systems having three or more relevant rings
- C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
- C07D311/82—Xanthenes
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/10—Non-macromolecular compounds
- C09K2211/1003—Carbocyclic compounds
- C09K2211/1007—Non-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/10—Non-macromolecular compounds
- C09K2211/1018—Heterocyclic compounds
- C09K2211/1025—Heterocyclic compounds characterised by ligands
- C09K2211/1088—Heterocyclic compounds characterised by ligands containing oxygen as the only heteroatom
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention relates to clinical treatment fields, more particularly to one kind to have following structure for detecting homocysteine structure fluorescence probe para-compound and its application：By the measurement to fluorescence intensity can effectively find out this probe can and homocysteine structure in amino and coloured glaze base group specificity generation nucleophilic addition, often the space structure of body changes altogether, fluorescence intensity in system is caused to enhance, alternative detection homocysteine, is not responding to cysteine and other amino acid.
Description
Technical field
The present invention relates to clinical treatment fields, more particularly to one kind is for detecting homocysteine structure fluorescence probe pair
Position compound and its application.
Background technique
Homocysteine (Homocysteine, HCY) is also known as homocysteine, is a kind of sulfur-containing amino acid and is not belonging to
20 kinds of amino acid of constitutive protein matter, cannot synthesize in vivo, can only derive from the catabolism of methionine (methionine).Normally
In the case of, blood HCY content is little, and general HCY level total on an empty stomach is 5umol/L-15umol/L；Slightly, the high HCY of moderate, severe
Mass formed by blood stasis patient's concentration is respectively 16umol/L-30umol/L, 31umol/L-100umol/L, is greater than 100umol/L.Research card
It is real：The HCY of high concentration is coronary artery disease, cranial vascular disease, the independent risk factor of peripheral artery disease, danger level with
The raising of concentration and increase.Therefore the concentration for monitoring and reducing homocysteine, has highly important clinical meaning.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art, provide a kind of detection homotype
Cysteine structure fluorescence probe para-compound and its application.
One kind having following structure for detecting homocysteine structure fluorescence probe para-compound：
One kind is used to detect homocysteine structure fluorescence probe para-compound application,
A. the dilution of compound
Above compound 0.1g is weighed in 1.5mlEP pipe, 1ml distilled water is added, is dissolved, is mixed, gradient dilution to 300
Times；
B. the fluoremetry of compound
96 hole black microwell plates are taken, above compound each 100 μ l, exciting light 280nm is added, emits light 590nm, measures it
Fluorescence intensity；
C. the fluoremetry of homocysteine
96 hole black microwell plates are taken, 0 μm of ol of homocysteine, 1 μm of ol, 2 μm of ol, 5 μm of ol, 10 μm of ol, 20 μ is added
Homocysteine each 100 μ l addition each 100 μ l of above compound, the 37 degree of incubation 10min of mol, 50 μm of ol and 100 μm of ol,
Exciting light 280nm emits light 590nm, measures its fluorescence intensity.
The beneficial effects of the invention are as follows：
By the measurement to fluorescence intensity can effectively find out this probe can and homocysteine structure in amino and
The generation nucleophilic addition of coloured glaze base group specificity, often the space structure of body changes altogether, causes fluorescence intensity in system
Enhancing, alternative detection homocysteine, is not responding to cysteine and other amino acid.
Detailed description of the invention
Fig. 1 is the linear graph that probe is reacted with homocysteine.
Specific embodiment
In order to make those skilled in the art more fully understand technical solution of the present invention, with reference to the accompanying drawing and most
The present invention is described in further detail for good embodiment.
It is of the invention a kind of for detecting homocysteine structure fluorescence probe para-compound, there is following structure：
One kind is used to detect homocysteine structure fluorescence probe para-compound application,
A. the dilution of compound
Above compound 0.1g is weighed in 1.5mlEP pipe, 1ml distilled water is added, is dissolved, is mixed, gradient dilution to 300
Times；
B. the fluoremetry of compound
96 hole black microwell plates are taken, above compound each 100 μ l, exciting light 280nm is added, emits light 590nm, measures it
Fluorescence intensity；
C. the fluoremetry of homocysteine
96 hole black microwell plates are taken, 0 μm of ol of homocysteine, 1 μm of ol, 2 μm of ol, 5 μm of ol, 10 μm of ol, 20 μ is added
Homocysteine each 100 μ l addition each 100 μ l of above compound, the 37 degree of incubation 10min of mol, 50 μm of ol and 100 μm of ol,
Exciting light 280nm emits light 590nm, measures its fluorescence intensity.
Experimental material and equipment are cell imaging microwell plate analyzer (C5MVDG) BioTek, the U.S. in this example.
After probe is reacted with homocysteine, probe and half Guang ammonia of homotype is can be obtained in subtracted probe fluorescence autofluorescence
The linear graph (Fig. 1) of acid reaction.(coefficient R=0.9985)
Fluorescence intensity does repetitive test and obtains result after being reacted with homocysteine its probe：
It is high that test result stability can be obtained, experimental result is reliable.
By the measurement to fluorescence intensity can effectively find out this probe can and homocysteine structure in amino and
The generation nucleophilic addition of coloured glaze base group specificity, often the space structure of body changes altogether, causes fluorescence intensity in system
Enhancing, alternative detection homocysteine, is not responding to cysteine and other amino acid.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (2)
1. one kind is for detecting homocysteine structure fluorescence probe para-compound, which is characterized in that have following knot
Structure：
2. one kind is for detecting homocysteine structure fluorescence probe para-compound application, it is characterised in that：
A. the dilution of compound
Above compound 0.1g is weighed in 1.5mlEP pipe, 1ml distilled water is added, is dissolved, is mixed, gradient dilution is to 300 times；
B. the fluoremetry of compound
96 hole black microwell plates are taken, above compound each 100 μ l, exciting light 280nm is added, emits light 590nm, measures its fluorescence
Intensity；
C. the fluoremetry of homocysteine
96 hole black microwell plates are taken, 0 μm of ol of homocysteine, 1 μm of ol, 2 μm of ol, 5 μm of ol, 10 μm of ol, 20 μm of ol, 50 is added
Each 100 μ l of above compound, 37 degree of incubation 10min, exciting light is added in each 100 μ l of the homocysteine of μm ol and 100 μm of ol
280nm emits light 590nm, measures its fluorescence intensity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810606849.1A CN108822070A (en) | 2018-06-13 | 2018-06-13 | One kind is for detecting homocysteine structure fluorescence probe para-compound and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810606849.1A CN108822070A (en) | 2018-06-13 | 2018-06-13 | One kind is for detecting homocysteine structure fluorescence probe para-compound and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108822070A true CN108822070A (en) | 2018-11-16 |
Family
ID=64144894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810606849.1A Pending CN108822070A (en) | 2018-06-13 | 2018-06-13 | One kind is for detecting homocysteine structure fluorescence probe para-compound and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108822070A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105339438A (en) * | 2013-07-05 | 2016-02-17 | 富士胶片株式会社 | Pigment multimer, coloring composition, cured film, color filter, method for manufacturing color filter, solid-state imaging element, and image display device |
-
2018
- 2018-06-13 CN CN201810606849.1A patent/CN108822070A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105339438A (en) * | 2013-07-05 | 2016-02-17 | 富士胶片株式会社 | Pigment multimer, coloring composition, cured film, color filter, method for manufacturing color filter, solid-state imaging element, and image display device |
Non-Patent Citations (1)
| Title |
|---|
| SAMUEL SAS ET AL.: "Highly luminescent hybrid materials based on smectites with polyethylene glycol modified with rhodamine fluorophore", 《APPLIED CLAY SCIENCE》 * |
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|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181116 |
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| WD01 | Invention patent application deemed withdrawn after publication |