CN108815505A

CN108815505A - Improve the composition that middle-aged and the old's bone density and osteoarticular function avoid waist-leg from knotting

Info

Publication number: CN108815505A
Application number: CN201810705237.8A
Authority: CN
Inventors: 傅金荣
Original assignee: Tianyuan Jiangxi Pharmaceutical Co Ltd
Current assignee: Tianyuan Jiangxi Pharmaceutical Co Ltd
Priority date: 2018-07-01
Filing date: 2018-07-01
Publication date: 2018-11-16
Anticipated expiration: 2038-07-01
Also published as: CN108815505B

Abstract

The present invention relates to the compositions that raising middle-aged and the old's bone density and osteoarticular function avoid waist-leg from knotting.The composition is made of 500 parts by weight of kudzu root extract, 400~500 parts by weight of aminoglucose hydrochloride, 300~500 parts by weight of calcium carbonate, 50~150 parts by weight of chondroitin sulfate, 10~40 parts by weight of casein phosphopeptide and the acceptable carrier of optional physiology.Test confirms that the present composition has the effect of excellent raising middle-aged and the old bone density, enhancing osteoarticular function and waist-leg is avoided to knot.The invention further relates to the preparation method of above-mentioned composition and they preparation for improve middle-aged and the old's bone density, osteoarticular function and avoid waist-leg knot product in purposes.

Description

Improve the composition that middle-aged and the old's bone density and osteoarticular function avoid waist-leg from knotting

Technical field

The invention belongs to medicines and health protection product technical fields, are related to a kind of for improving bone density for the middle-aged and the old, improving Osteoarticular function, avoid or reduce waist-leg cramp Chinese medicine composition, further relate to this composition preparation method and they Medical treatment, medical purposes, more particularly to one kind composition made of casein and pueraria lobata.

Background technique

It has different degrees of bone density after people to person in middle and old age to lose, associated physiological performance is osteoarticular function It reduces, and waist-leg cramp often occurs.

Bone density (bone mineral density, BMD) full name is bone mineral density, is one of bone strength Important indicator is indicated with gram/cubic centimeter, is an absolute value.In clinical use bone density value since different bones is close The absolute value for spending detector is different, judges whether bone density is normal usually using T value.T value is a relative value, normal reference value Between -1 and+1.It is abnormal when T value is lower than -2.5.Bone density is an important symbol of bone mass, reflects sclerotin Porousness predicts the important evidence of fracture risk.Due to the exploitation of measurement method being increasingly modified with advanced software, make this Method can be used for different parts, and measurement accuracy significantly improves.In addition to diagnosable osteoporosis, still seen for clinical drug effect It examines and epidemiological survey, has significant superiority in terms of predicting osteoporotic fracture.Northern Han Nationality Healthy People Bone density value determines the normal value of peak bone mount age, size and each age group.Lumbar vertebrae L2--L4 and hip are measured using DXA Portion's bone density.The result shows that male's peak bone mount age each position is at 20--24 years old, L2--L4 density value is 1.228 (g/ cm3)；Women summit-value-age lumbar vertebrae was worth at 30--34 years old for 1.197 (g/cm3).The femoral neck bone mineral density summit-value-age is in 25--29 Year.In the world's osteoporosis conference in 2004 of international osteoporosis foundation (IFO), Univ Sheffield UK WHO metabolism Property osteopathy research center Johnell etc. meta-analysis are carried out to 12 clinical researches after think, no matter male or women, bone Density (BMD) is highly important risk of fractures factor.30,000 9 thousand people in 12 cohort studies are included in the research, observe altogether About 170,000 man-years.The influence caused danger to fracture of BMD in each study population is divided respectively using Poisson model Analysis, merges analysis to each result of study using weighting coefficient.The results show that BMD is fine for male and female Fracture (especially Hip Fracture) prediction index.In 65 years old age group, BMD value 1 standard deviation (SD) of every reduction, male's hip The dangerous of portion's fracture increases by 2.94 times (2.02--4.27), and women increases by 2.88 times (2.31--3.59).But this effect is in Age-dependent, 50 years old dangerous gradient are significantly higher than 80 years old.The danger ladder of various types of fracture and osteoporotic fracture Degree is below Hip Fracture, and BMD predictive value increases with the increase at age.In 65 years old age group, BMD is every to reduce by 1 SD, the dangerous of osteoporosis fracture increase by 1.41 times of (1.33--1.51) women increases, 1.38 times (1.28--1.41). For Hip Fracture, fracture extends with measurement BMD interval time, and the predictive value of BMD reduces, but does not have conspicuousness.BMD value is got over It is low, predict osteoporotic fracture (and various types fracture) effect it is bigger, T value reduce by 4 SD when hazard ratio be 2.10 Hazard ratio is 1.73 (1.59--1.89) when (1.63--2.71) T value reduces by 1 SD.For Hip Fracture, the predicting function of BMD Also similar.Johnell etc. thinks, due to selected clinical research be it is international, the conclusion which is obtained There is good application value.The analysis the result shows that, BMD can be used for the screening of susceptible case, still, in application process, Influence BMD fracture prediction is worth in view of the age.It is former can to lack generation according to bone density for the method for improving bone density Different material may be selected when carrying out Ricipe for health care food design for the difference of cause and the mechanism of action.The raw material being commonly used is as follows： 1. calcium agent：It is daily to give 1.00 grams~1.50 grams if calcium uptake is normal.In various calcium agent, calcium carbonate uses commonplace. 0.75 gram~2.5 grams daily to over-65s old man.To using more than estrogen side effect and have the possibility for inducing carcinoma of endometrium Person, give large dosage calcium, can play the role of with use estrogen it is identical, kidney stone patient cannot take in a large amount of calcium.2. Vitamin D and its activated product：It is considered that senile osteoporosis patient is often accompanied by vitamin d insufficiency, therefore advocate to give more Vitamin D, actually in addition to being associated with osteomalacia (in general, only children are susceptible to suffer from osteomalacia, such as rickets), intestines calcium uptake Obstacle and vitamin D metabolites generate reduction person, general to be not necessarily to supplement a large amount of vitamin Ds, truly have above-mentioned three kinds of situation persons, can Vitamin D is given simultaneously.3. calcitonin：Calcitonin can reduce bone absorption, reduce the calcium in blood circulation, increase the calcium in sclerotin Content, calcitonin is due to that can reduce blood calcium, so should supply calcium amount when with calcitonin, play the role for the treatment of osteoporosis. 4. phosphoric acid salt：Phosphoric acid salt treatment osteoporosis is developed in recent years, and phosphate can promote bon e formation, inhibit osteocyte It destroys, it can be with prolonged application.5.n-3 polyunsaturated fatty acid (alpha-linolenic acid)：N-3 fatty acid influences the bone metabolism of the mankind, leads to It crosses different mechanism of action to play regulatory role osteoblast and osteoclast, strengthens n-3 fatty acid and be conducive to improve bone density.

Bones and joints are connected by the capsule for borrowing connective tissue to constitute between adjacent bone.Bone and bone are indirectly connected with title Bones and joints.Phase Pair bone face between have lacuna, it is intracavitary to contain a small amount of synovia.Its movable amplitude is larger, and there are articular surface, joint in each joint Capsule, articular cavity.There are also the supplementary structures such as ligament, articular disc and meniscus in certain joints.Human body why can activity freely, just It is because there is the structure of Bones and joints, most Bones and joints are not only to provide the need of physical activity, and via cartilage protection bone It avoids wearing.About the maintenance of Bones and joints, hydroxy-proline is the delivery vehicle that calcium is transported in blood plasma to osteocyte, in osteocyte Ossein be hydroxyapatite binder, it and hydroxyl imitate apatite and together constitute the main body of bone.Therefore, as long as intake Enough collagens, it is ensured that the intake that normal body is calcareous, collagen can be at being made the health food replenished the calcium.Bone Arthropathy is the clinical findings by the acute and chronic damage of bone articular cartilage or based on cartilage degeneration, and osteoarthropathy is most of Occur in the elderly, for osteoarthropathy in addition to articular cartilage damage regression, it is orthopaedics that the later period, which changes there is also Bones and joints structural relation, Outpatient service frequently-occurring disease, common disease.Osteoarthropathy is a kind of with local cartilage degeneration, and bone loss, marginal spurs are formed And the chronic joint diseases that joint deformity and subchondral bone matter densification are characterized, also known as osteoarthritis, degenerative osteoarthropathy increase Natural disposition arthritis, senescent arthritis.It is apt to occur in 50 years old or more crowd, women is more than male, in the different degrees of influence of this disease The quality of life of gerontal patient.Nearly all case has different degrees of pain, is slowly in progress with the course of disease.Main performance joint Pain is obvious when coming into play, slightly pain relief after activity, however when weight bearing and Bones and joints hyperactivity, pain can aggravate again, The characteristics of this is osteoarthropathy.Sometimes pain can be in radioactivity, and if hip arthralgia is radiation to femoribus internus, knee joint is attached Closely.Early stage visible joint is stiff, as knee joint for a long time be in a certain position when, consciously activity unfavorable, starting difficulty, after gradually There is joint instability, joint flexion-extension motion range reduces and walking ability decline, and it especially with up/down steps, squats down, runs, the abilities such as jump Decline more obvious.Some osteoarthropathy end-stage patients are also possible that some lower limb malformations, most common with out knee, that is, are commonly called as " bowlegs ".It is main to consider following several points in terms of the treatment of osteoarthropathy：1. functional training：Reasonable exercise can restore flesh Meat convergent force, joint flexibility ratio and prevention and treatment osteoporosis, unreasonable exercise then will increase joint load, cause cartilage into one Step damage, to aggravate clinical symptoms.Some patients can usually be seen blindly to carry out walk long distances, do disco, or even run The exacerbation of symptoms after uncomfortable exercise such as walk, climb the mountain.We advocate exercise should as far as possible in the case where joint is not born a heavy burden flexion-extension motion, it is proposed that it is strong Limb on the spot bears a heavy burden, and the activity of suffering limb bend and stretch joint or seat carry out joint and bend and stretch exercise.Joint can be aggravated by trying not to squat down etc. The activity of load.It can practice sit-ups, leg raising straight etc. in bed for hip joint, knee joint, number is The more the better.Trip Swimming is the sports events for being very suitable to knee osteoarthritis patient, it without great burden, can be such that muscle fills at knee joint The activity divided.But it is capable that breaststroke requires knee joint to use twisting.Sometimes will cause it is bad as a result, therefore be proposed with freestyle swimming, Backstroke.2. the consciousness of improving the precaution：Patient it is to be understood that the harmfulness of the disease and the importance of early treatment, improve patient to it is dangerous because The understanding of element, eliminates and avoids pathogenic factor, is conducive to the recovery for controlling disease and function, while to establish the letter of Fighting Disease The heart.3. Saving cortilage：Joint weight-bearing activities should be limited, avoid standing too long or long walk, walking stick can be used with mitigate by Tired joint load；Overweight person preferably loses weight；It is noted that diseased joints are warming, wind sheltering is cold；It can lie in bed in short term not when serious Breath is braked completely.4. local physical therapy：The fever of acute stage joint, swelling preferably first carry out local cold compress, can be using heat after detumescence of bringing down a fever It applies.Chronic phase, can also be using infrared ray, ultrashort wave, acupuncture, wax therapy, massage etc..

Waist-leg knots, and scientific name is a kind of idiomuscular mandatory contraction muscle cramp.Leg cramps occur mainly in small The muscle of leg and toe, when breaking-out, are in great pain, and often people's pain is waken up when especially midnight knots, is difficult to relieve pain for a long time, shadow Ring sleep.The reason of waist-leg knots mainly has：1. cold stimulation.If winter takes exercise in cold environment, warming-up exercise is not filled Point；Summer swimming water temperature is lower, all easily causes leg cramps.Sleep does not cover quilt at night, and Calf muscle is by cold stimulation, meeting Spasm must allow people's pain to be waken up.2. muscle continuously shrinks too fast.When strenuous exercise, whole body is in tension, and leg muscle was shunk Fastly, the time loosened is too short, and local metabolic product lactic acid increases, and the contraction of muscle is difficult to coordinate with loosening, so as to cause shank Muscle cramp.3. excessive sweating.Run duration is long, and amount of exercise is big, perspires more, and salinity is not replenished in time, internal liquid with Electrolyte is largely lost, and metabolic waste accumulation, the blood circulation of muscle part is bad, is also easy to happen spasm.4. excessive fatigue. When long-distance travel, climbing the mountain, ascending a height, Calf muscle is easiest to that fatigue occurs.It is all that a foot supports complete because ascending a height each time Body weight, strength needed for the muscle of this leg lifts foot will be six times of human body weight, when its fatigue to a certain extent when, will Spasm occurs.5. calcium deficiency.In muscle contraction, calcium ion plays an important role.When calcium ion concentration is too low in blood, Muscle is easy excited and spasm.Adolescent growth blastoprolepsis, it is easy to calcium deficiency, therefore leg cramps just often occur.6. sleeping Poor position.If lain on the back for a long time, so that quilt is pressed in instep or long-time prostrate, be against instep on bed, force shank A little muscle are in absolute relaxation state for a long time, cause muscle " passive contracture ".7. often frequently occurring to have with angiosis It closes.

Those skilled in the art still expect to have new method improve middle-aged and the old's bone density, enhancing osteoarticular function, And waist-leg is avoided to knot.Those skilled in the art still expect have new drug or health product to for improving the middle-aged and the old Bone density, enhancing osteoarticular function simultaneously avoid waist-leg from knotting.

Summary of the invention

The purpose of the present invention is to provide a kind of raising middle-aged and the old bone density, enhancing osteoarticular function and avoid waist-leg The new method of cramp, another object of the present invention is to provide a kind of new drugs or health product to for improving person in middle and old age People's bone density, enhancing osteoarticular function simultaneously avoid waist-leg from knotting, it has been unexpectedly discovered that combination obtained by prescription of the present invention Excellent raising middle-aged and the old bone density, enhancing osteoarticular function and the function of avoiding waist-leg from knotting is presented in object, and is presented one Kind or a variety of excellent technical effects.It is accomplished the present invention is based on this discovery.

For this purpose, first aspect present invention provides, one kind is used to improve middle-aged and the old's bone density and osteoarticular function avoids waist The composition of leg cramps comprising：Kudzu root extract, aminoglucose hydrochloride, calcium carbonate, chondroitin sulfate, casein phosphoric acid Peptide and the acceptable carrier of optional physiology.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition comprising：500 parts by weight of kudzu root extract, 400~500 parts by weight of aminoglucose hydrochloride, calcium carbonate 300~500 Parts by weight, 50~150 parts by weight of chondroitin sulfate, 10~40 parts by weight of casein phosphopeptide and optional physiology can connect The carrier received.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition comprising：500 parts by weight of kudzu root extract, 420~470 parts by weight of aminoglucose hydrochloride, calcium carbonate 350~450 Parts by weight, 75~125 parts by weight of chondroitin sulfate, 20~30 parts by weight of casein phosphopeptide and optional physiology can connect The carrier received.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition comprising：500 parts by weight of kudzu root extract, 440 parts by weight of aminoglucose hydrochloride, 400 parts by weight of calcium carbonate, sulphur Aching and limp 100 parts by weight of ossein, 25 parts by weight of casein phosphopeptide and the acceptable carrier of optional physiology.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition, in the form of tablet or granule.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition, in the form of tablet, the acceptable carrier of physiology includes xylitol, microcrystalline cellulose, magnesium stearate.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition, in the form of tablet, in terms of every 500 parts by weight kudzu root extract, the amount of xylitol is 500~1000 parts by weight, Such as 600~900 parts by weight, such as 700~800 parts by weight, such as 760 parts by weight.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition, in the form of tablet, in terms of every 500 parts by weight kudzu root extract, the amount of microcrystalline cellulose is 200~300 weight Part, such as 220~280 parts by weight, such as 240~260 parts by weight, such as 250 parts by weight.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition, in the form of tablet, in terms of every 500 parts by weight kudzu root extract, the amount of magnesium stearate is 20~30 parts by weight, example Such as 22~28 parts by weight, such as 24~26 parts by weight, such as 25 parts by weight.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition is prepared according to the method included the following steps：

(1) it is uniformly mixed kudzu root extract with aminoglucose hydrochloride, separately mixes calcium carbonate and chondroitin sulfate equal It is even；

(2) so that casein phosphopeptide is added the water dissolution of 5~8 times of amounts, add 2~3 times of casein phosphopeptide weight of wood Sugar alcohol is uniformly mixed, makes this mixture ventilation process 2.5~3 hours at a temperature of 60~70 DEG C, is uniformly mixed；

(3) various mixtures obtained by both the above step are mixed, adds the wood of microcrystalline cellulose and surplus thereto Sugar alcohol is uniformly mixed, and adding water is lubricant, prepares wet granular, keeps wet granular dry；

(4) it is uniformly mixed dry particle obtained by previous step with magnesium stearate, composition is obtained, as particle system Dosage form formula is directly packed, or is further pressed into tablet, obtains the dosage form of granule or tablet.

According to a first aspect of the present invention for improving middle-aged and the old's bone density and osteoarticular function avoids waist-leg from knotting Composition is the dosage form of tablet.It in one embodiment, is the tablet form of chewable tablets.

It has been unexpectedly discovered that being mixed again with 2~3 times of amount xylitols after so that casein phosphopeptide is formed solution, connect Ventilation process 2.5~3 hours at a temperature of 60~70 DEG C, thus composition made from mode (ratio in casein phosphopeptide Without being handled by this method with xylitol) it can be to avoid the discoloration of drug after a long time placement.Specific experiment is：Respectively refer to reality 1~embodiment of example 5 is applied, different is only to be uniformly mixed casein phosphopeptide with corresponding amount xylitol in step (2), this is mixed It closes object and is directly used in subsequent step, obtaining 5 batches can be referred to as to supplement example 1~supplement example 5 tablet；Make embodiment 1~reality It applies 5 tablet of example and supplement example 1~5 tablet of supplement example was placed at 40 DEG C of temperature up to 6 months, make to add with tablet in June for 0 month water-soluble Solution, filtering, the solution comprising 20 μ g/ml of casein phosphopeptide is made, and (solution colour of Examples 1 to 5 is obviously than supplement after June Example 1~5 it is of light color), at 360nm measure absorbance；Its absorbance is calculated as follows for each sample and increases percentage Number：Absorbance increases percentage=[- 0 month absorbance of absorbance in June] 0 month absorbance × 100% of ÷；As a result, embodiment 1~reality It is 3.3%~7.4% that the absorbance for applying 5 tablet of example, which increases percentage, and supplement example 1~5 tablet of supplement example absorbance increases by hundred Score is 36.7%~47.2%, and the latter, which shows, is higher than the former, shows that the latter's tablet is easier discoloration, color burn.

Further, second aspect of the present invention provides a kind of prepare for improving middle-aged and the old's bone density and Bones and joints function The method for being avoided that the composition that waist-leg knots, the composition include：Kudzu root extract, aminoglucose hydrochloride, calcium carbonate, Chondroitin sulfate, casein phosphopeptide and xylitol, microcrystalline cellulose, magnesium stearate, this method comprises the following steps：

Method according to a second aspect of the present invention, wherein described for improving middle-aged and the old's bone density and osteoarticular function is kept away Exempting from the composition that waist-leg knots includes：500 parts by weight of kudzu root extract, 400~500 parts by weight of aminoglucose hydrochloride, carbonic acid 300~500 parts by weight of calcium, 50~150 parts by weight of chondroitin sulfate, 10~40 parts by weight of casein phosphopeptide, and optional The acceptable carrier of physiology.

Method according to a second aspect of the present invention, wherein described for improving middle-aged and the old's bone density and osteoarticular function is kept away Exempting from the composition that waist-leg knots includes：500 parts by weight of kudzu root extract, 420~470 parts by weight of aminoglucose hydrochloride, carbonic acid 350~450 parts by weight of calcium, 75~125 parts by weight of chondroitin sulfate, 20~30 parts by weight of casein phosphopeptide, and optional The acceptable carrier of physiology.

Method according to a second aspect of the present invention, wherein described for improving middle-aged and the old's bone density and osteoarticular function is kept away Exempting from the composition that waist-leg knots includes：500 parts by weight of kudzu root extract, 440 parts by weight of aminoglucose hydrochloride, calcium carbonate 400 Parts by weight, 100 parts by weight of chondroitin sulfate, 25 parts by weight of casein phosphopeptide and the acceptable carrier of optional physiology.

Method according to a second aspect of the present invention, wherein described for improving middle-aged and the old's bone density and osteoarticular function is kept away In the composition for exempting from waist-leg cramp, in terms of every 500 parts by weight kudzu root extract, the amount of xylitol is 500~1000 parts by weight, example Such as 600~900 parts by weight, such as 700~800 parts by weight, such as 760 parts by weight.

Method according to a second aspect of the present invention, wherein described for improving middle-aged and the old's bone density and osteoarticular function is kept away In the composition for exempting from waist-leg cramp, in terms of every 500 parts by weight kudzu root extract, the amount of microcrystalline cellulose is 200~300 weight Part, such as 220~280 parts by weight, such as 240~260 parts by weight, such as 250 parts by weight.

Method according to a second aspect of the present invention, wherein described for improving middle-aged and the old's bone density and osteoarticular function is kept away In the composition for exempting from waist-leg cramp, in terms of every 500 parts by weight kudzu root extract, the amount of magnesium stearate is 20~30 parts by weight, example Such as 22~28 parts by weight, such as 24~26 parts by weight, such as 25 parts by weight.

Further, third aspect present invention is provided including kudzu root extract, aminoglucose hydrochloride, calcium carbonate, sulphur The composition of aching and limp ossein, casein phosphopeptide and the acceptable carrier of optional physiology, in preparation for old in improving The purposes in product that year people's bone density and osteoarticular function avoid waist-leg from knotting.

Purposes according to a third aspect of the present invention, the composition include：500 parts by weight of kudzu root extract, aminoguanidine hydrochloride 400~500 parts by weight of glucose, 300~500 parts by weight of calcium carbonate, 50~150 parts by weight of chondroitin sulfate, casein phosphoric acid 10~40 parts by weight of peptide and the acceptable carrier of optional physiology.

Purposes according to a third aspect of the present invention, the composition include：500 parts by weight of kudzu root extract, aminoguanidine hydrochloride 420~470 parts by weight of glucose, 350~450 parts by weight of calcium carbonate, 75~125 parts by weight of chondroitin sulfate, casein phosphoric acid 20~30 parts by weight of peptide and the acceptable carrier of optional physiology.

Purposes according to a third aspect of the present invention, the composition include：500 parts by weight of kudzu root extract, aminoguanidine hydrochloride 440 parts by weight of glucose, 400 parts by weight of calcium carbonate, 100 parts by weight of chondroitin sulfate, 25 parts by weight of casein phosphopeptide, and The acceptable carrier of optional physiology.

Purposes according to a third aspect of the present invention, the composition is in the form of tablet or granule.

Purposes according to a third aspect of the present invention, the composition is in the form of tablet, the acceptable load of physiology Body includes xylitol, microcrystalline cellulose, magnesium stearate.

Purposes according to a third aspect of the present invention, the composition in terms of every 500 parts by weight kudzu root extract, xylitol Amount is 500~1000 parts by weight, such as 600~900 parts by weight, such as 700~800 parts by weight, such as 760 parts by weight.

Purposes according to a third aspect of the present invention, the composition is in terms of every 500 parts by weight kudzu root extract, microcrystalline cellulose The amount of element is 200~300 parts by weight, such as 220~280 parts by weight, such as 240~260 parts by weight, such as 250 parts by weight.

Purposes according to a third aspect of the present invention, the composition is in terms of every 500 parts by weight kudzu root extract, magnesium stearate Amount be 20~30 parts by weight, such as 22~28 parts by weight, such as 24~26 parts by weight, such as 25 parts by weight.

Purposes according to a third aspect of the present invention, the composition are prepared according to the method included the following steps：

In the method and step that describes of the present invention, although the specific steps of its description are in certain details or language description Step different from described in the upper and example of following detailed description part, however, those skilled in the art according to The detailed disclosure of full text of the present invention can summarize approach described above step completely.

Any embodiment in either present invention face can be combined with other embodiments, as long as they are not It will appear contradiction.In addition, any technical characteristic can be adapted for other realities in any embodiment of either side of the present invention The technical characteristic in scheme is applied, as long as they are not in contradiction.

The invention will be further described below.

All documents recited in the present invention, their full content are incorporated herein by reference, and if these are literary When offering expressed meaning and the inconsistent present invention, it is subject to statement of the invention.In addition, the various terms that use of the present invention and Phrase has that well known to a person skilled in the art general senses, nonetheless, the present invention remain desirable at this to these terms and Phrase is described in more detail and explains, the term and phrase referred to is if any inconsistent with common art-recognized meanings, with institute's table of the present invention Subject to the meaning stated.

Pueraria lobata is legume pueraria lobata Pueraria lobata (Willd.) Ohwi or sweet kudzu Pueraria The dry root of thomsonii Benth..Autumn, the excavation of two season of winter, elegant jessamine take advantage of fresh-cut into sheet or fritter more；It is dry；Sweet kudzu is practised Claim " Pachyrhizua angulatus ", mostly removing crust, after being smoked with Sulfur, slightly dry, intercept or again longitudinal sectional two halves are dry.Elegant jessamine：In longitudinal sectional rectangle Sheet or small cube, long 5~35cm, 0.5~1cm of thickness.Crust light brown has vertical wrinkle, coarse.Section yellow-white, texture is not Obviously.Matter is tough, and fibroid is strong.It is odorless, it is slightly sweet.Pachyrhizua angulatus：Cylindrical, class spindle or semi-cylindrical, long 12~15cm, directly 4~8cm of diameter；Some is longitudinal sectional or beveling sheet, not of uniform size.Surface yellow-white or light brown, do not remove crust is in greyish brown Color.Cross section is visible by fibroplastic light brown proper alignment ring grain, and longitudal section is visible by several fibroplastic longitudinal grins.Body Weight, matter is hard, rich mealiness.Pueraria lobata is sweet, pungent, and taste is cool.Returns spleen, stomach meridian.It brings down a fever, promotes the production of body fluid with expelling pathogenic factors from muscles and skin, promoting eruption, the function of Shengyang Zhixie Effect.It is thirsty for fever caused by exogenous pathogens headache, stiff nape and back, it quenches one's thirst, measles without adequate eruption, hot dysentery, diarrhea；Hypertension neck pain.Pueraria lobata Isoflavone-containing ingredient Puerarin, Puerarin xyloside, daizeol, daidzin and cupreol, arachidic acid, and contain volume Starch (content is 19~20% in fresh pueraria lobata).The dry root starch-containing 37% of sweet kudzu.The root of trilobated leaf elegant jessamine rattan is containing shallow lake Powder 15~20%.Isolated from the root of the congener of India Puerarin, daizeol, daidzin, cupreol, 4 ', 6 "-diacetyl Puerarin and stigmasterol.It has been reported that Radix Puerariae (daidzein) containing daidzein, daidzin (daidzin), Puerarin (puerarin), 4 '-methoxy puerarin (4 '-methoxypuerarin), daidzein -4 ', 7- Diglucoside (daidzein-4 ', 7-diglucoside), daidzein -7- (6-O- malonyl)-glycoside [daidzein-7- (6-O-malonyl)-lucoside], genistein (genistein), formoononetin (for- Mononetin), daidzein -8-C- celery glycosyl (l → 6)-glycoside [daidzein-8-C-apiosyl (1 → 6) - Glucoside], genistein -8-C- celery glycosyl (1 → 6)-glycoside [genistein-8-C-apiosyl (1 → 6) - Glucoside], Puerarin xyloside (puerarinxyloside, PG-2), 3'-hydroxypuerarin (3 '- Hydroxypuerarin, PG-1), 3 '-methoxy puerarins (3 '-methoxypuerarin, PG-3), 4 '-O- glucosyl groups Puerarin (4 '-O-glucosyl puerarin, PG-6), pueraria lobata phenol (puerarol), pueraria lobata glucoside (pueroside) A, B, thorn Onocerin -7- glycoside (formononetin-7-glucoside), lupenone (lupenone), cupreol (β - Sitosterol), behenic acid (docosanoic acid), lignoceric acid (tetracosanoic acid), l- 20 Four alkanoic acid glyceride (glucerol-1-monotetracosanoate), allantoin (allantoin), the Portugal cupreol-β-D- Grape glucoside (β-sitosteryl- β-D-glucoside), 6,7- dimethoxy coumarins (6,7-dimethoxycoumarin), 5- methyl hydantoin (5-methylhydantoin) and with sophoradiol (sophoradiol), cantoniensis triol (cantoniensistriol), soyasapogenol (soyasapogenol) A, B, pueraria lobata soap alcohol (kudzusapogenol) C, A and Pueraria lobata soap alcohol B methyl esters (kudzusapogenol B methylester) is the triterpenoid saponin of glucoside member.Kudzu also contains daidzin Member, daidzin, Puerarin and cupreol.Sweet kudzu root contain daidzin, Puerarin, 4 '-methoxy puerarin, daidzein and Trace daidzein -4,7 '-diglucoside.The pharmacological action of pueraria lobata mainly has following aspect：1. the effect to the circulatory system, The flavones proposed in pueraria lobata can increase brain and coronal vascular flow amount.After anaesthetizing dog Internal carotid artery injection kudzu root flavone, brain blood flow Amount increases, and vascular resistence accordingly reduces, and effect maintains 2~20 minutes, is such as injected intravenously, then cerebral blood flow (CBF) increase is lighter, also not Adrenaline can be released and norepinephrine shrinks cerebrovascular effect, but brain can then be improved to hypertensive arteriosclerosis patient Circulation, action temperature and.Kudzu root flavone and kudzuvine root wine medicinal extract are injected in the coronary artery and vein of dog, can make coronary vasodilator blood Flow increases, and vascular resistence reduces.To rat abdominal cavity and subcutaneous injection kudzuvine root wine medicinal extract and its water decoction and pueraria lobata is injected intraperitoneally The crystallization of middle proposition has protective effect to the reaction of heart ischemia caused by pituitrin, and pueraria lobata water decoction is oral to height Blood pressure dog is without apparent antihypertensive effect.It is reported that there is excited and inhibition two kinds of ingredients of heart in pueraria lobata (Japan produces) decoction.2. solving Convulsion effect, the equal flavones of beans containing day in the commercially available pueraria lobata of Chinese pueraria lobata (kind is not specified) and Japan, to mouse, guinea pig in vitro intestinal tube tool Have a papaverine sample spasmolysis, but in the domestic more Japanese production person's spasmolysis of pueraria lobata it is strong, may with contained big and flavones is more has It closes, this spasmolysis ingredient can be to the effect of antihistaminicum and acetylcholine；Other osajins isolated in pueraria lobata are produced from Japan Derivative then expands acetylcholine effect without apparent.Furthermore the substance that elbow intestinal tube has contraction is still isolated, Japan produces pueraria lobata The MTF-101 of middle proposition has muscarinic action.3. hypoglycemic effect, pueraria lobata (kind is not specified) decoction is oral to rabbit, opens Begin 2 hours blood glucose rises, and declines immediately, decline in the 3rd, 4 hour is minimum, not only makees without confrontation to rabbit adrenaline hyperglycemia With being allowed to increase instead, but blood glucose can be promoted to restore normal ahead of time.Under pueraria lobata water extract can also make after Rabbits ' Blood Sugar just rising Drop, more significant to the effect of hungry osmium rabbit blood glucose increasing, ether extract then has no significant effect glycometabolism.4. antipyretic and estrogen-like Effect, Japan, which produces pueraria lobata preserved material, has obvious refrigeration function to artificial fever rabbit, maintains 4~5 hours as long as.Pueraria lobata can increase not The weight of mature Mouse Uterus, there is estrogen-like action.The effective component of this effect is daizeol.Pueraria lobota used in the present invention Its regulation for meeting national drug standards YBZ013422006-2009Z of root extract, also referred to as Pueraria Flavonid, this Pueraria lobota Root extract is the extract extracted by pueraria lobata through water, there is sale of finished goods on the market at present.

Glucosamine, Glucosamine, also known as D- Glucosamine also have usually using its hydrochloride and use it Sulfate.Glucosamine is the compound that a hydroxyl of glucose is replaced by an amino.Molecular formula C6H13O5N, custom Claim amino sugar, abbreviation ammonia sugar.Also known as gucosamine, be widely present in nature, 2-amino-2-deoxy-D-Glucose usually with N- acetyl derivative (such as chitin) is present in micro- life in the form of N- sulfuric ester and N- acetyl -3-O- lactic acid ether (muramic acid) In object, the polysaccharide of animal origin and combination polysaccharide.Mainly applicable degenerative osteoarthritis of the knee, osteoproliferation, meniscus injury. Glucosamine, it is the substance synthesized in human body, is the important nutrient to form cartilage cell, is the day of healthy articular cartilage Right morphological element.With advancing age, the shortage of the intracorporal Glucosamine of people is increasingly severe, and articular cartilage is constantly degenerated And abrasion.A large amount of medical researches in the U.S., Europe and Japan show：Glucosamine can help to repair and safeguard cartilage, and The growth of cartilage cell can be stimulated.The major function of Glucosamine has：It is pain caused by releiving because of arthritis, stiff and swollen It is swollen：Osteoporosis consumes cartilage, eventually leads to fragmentation peeling, joint has lacked the buffering of cartilage, has been also easy to produce stiff and inflammation. And gucosamine helps to repair damaged cartilage, stimulates the generation of new cartilage, improves inflammation symptom, it is arthralgia of releiving, stiff And swelling.Strengthen cartilage structure, prevention function of joint failure：With body ages, joint tissue can be seriously worn, gucosamine It can protect and strengthen cartilage structure, prevent the function of joint generated by joint ageing failure.Lubricating joint and maintenance joint Function：Gucosamine can manufacture proteoglycan lubricating joint, prevent Bones and joints friction pain, make joint motion freely.Amino Portugal The treatment function of grape sugar mainly has：1, degenerative osteoarthritis of the knee, glucosamine in treating gonitis mainly pass through reparation Articular cartilage expedites the emergence of knuckle synovia, makes that rigid friction no longer occurs between articular surface, pain, swelling, bony crepitus no longer occurs Etc. symptoms so that joint space is restored normal, function of joint is thoroughly restored and by the reparation to articular cartilage.2, sclerotin Hyperplasia, exogenous intake Glucosamine make intra-articular aminoglucose sugared content restore balance state, and stimulation cartilage cell synthesizes Proteoglycan and collagenous fibres generate cartilage matrix, repair damaged cartilage, improve articular cartilage itself repair ability, pass through Glucosamine repairing articular cartilage expedites the emergence of knuckle synovia, plays essence to osteoproliferation arthritis (osteoarthritis) and controls Treatment effect.3, meniscus injury, Glucosamine can repair the meniscus of damage, while repair the secondary pass of meniscus injury Nodal section Cartilage breakdown, fundamentally repairs joint injury.Glucosamine can remove injurious factor, inhibit and eliminate joint cunning Film inflammatory reaction.4, Chondromalacia of patella supplements Glucosamine exogenously, and the joint that can be repaired between kneecap and femur is soft Bone.5, cervical spondylotic radiculopathy.6, cervical spondylosis myeloid form.7, Cervical Spondylosis.8, cervical spondylosis adrenergic type, amino Portugal Grape sugar eliminates soft tissue oedema by the inflammatory reaction of inhibition articuli intervertebrales soft tissue, and repairs damaged cartilaginous ring, makes intervertebral Disk protrusion is not easy to happen suddenly, and promotes knuckle synovia normal secretions, keeps neck activity more flexible.9, synovitis, Glucosamine By removing harmful substance in articular cavity, the diseases such as pain, swelling can also be slowed down by the reparation to articular cartilage and lesion synovial membrane Shape.10, Hand osteoarthritis supplements Glucosamine, can repair the articular cartilage of abrasion, mitigates since articular cartilage abrasion is made At arthralgia.11, sufficient osteoarthritis, after taking Glucosamine, Glucosamine can repairing articular cartilage abrasion, such as It takes for a long time, for the ankle-joint of the sportsman of frequent strenuous exercise, there is good protective effect.Scapulohumeral periarthritis, Glucosamine By promoting the synthesis of proteoglycan and collagenous fibres, the soft tissues such as tendon, ligament, synovial membrane, synovial bursa are repaired, pass is expedited the emergence of Section and synovial bursa synovia release soft tissue of joint adhesion, shoulder mobility function are enable to restore.The protrasion of the lumbar intervertebral disci, supplement Glucosamine can obviously improve the degeneration of articuli intervertebrales cartilage and interverbebral disc anulus fibrocartilagineus, mitigate the pain of patient.Wind Moist, arthritis, rheumatic arthritis will lead to the destruction of articular cartilage, supplement Glucosamine, can repairing articular cartilage Abrasion, mitigates the pain of patient.15, the calcium ion, enhancing bone tissue growth and health of somagenic need are supplemented.Glucosamine is one The derivative of kind native amino monosaccharide, is important component necessary to synthetic proteins glycan in cartilage matrix.Proteoglycans can be with Articular cartilage is set to have the function of absorbing impact force by inhibiting the drawing force of collagenous fibres.The morning of degenerative disease in joint Phase, it is increased for assembling the biosynthesis of dextran；In the later period of disease, then on the contrary.Thus lead to the elasticity of cartilage constantly Weaken and gradually appears arthritic many symptoms.Amino monosaccharide, which can stimulate cartilage cell to generate, has normal multimeric structure Glycoprotein inhibits some enzymes (such as clostridiopetidase A) for damaging articular cartilage, prevents cortin and certain nonsteroidal anti-inflammatory drugs The release of the endogenous toxic material prime factor of damage and reduction damaging cells to cartilage cell.In arthritic development process, supplement is outer The Glucosamine of source property may play beneficial effect.In vitro in test, if supplementing Glucosamine, cartilage is formed Polyblast can synthesize more aggregation dextrans.In arthritic animal model, Glucosamine also has Antioxidation inhibits the generation of the superoxide radical of damaging cells.By above-mentioned approach, Glucosamine plays direct Anti-inflammatory effect can be relieved the pain symptom of osteoarthritis, improve function of joint, and can prevent the development of the osteoarthritis course of disease.Ammonia Base glucose oral 90% is absorbed, and blood is permeated into rapidly by biological barrier, and be distributed to tissue and organ, especially to pass Section cartilage has compatibility, can permeate into articular cartilage matrix, reaches cartilage cell.4h blood concentration reach to peak value after oral.Medication 1~8h afterwards can measure Glucosamine concentration in liver, kidney, stomach wall, small intestine, brain, bone, muscle and articular cartilage and successively pass Increase.The ingredient declines for 24 hours.T1/2 is 18h.Glucosamine is lesser molecule through liver metabolism, is finally decomposed to titanium dioxide Carbon, water and urea.The 10% of oral dose is discharged from homaluria, 11% through excrement, remaining major part is with form of carbon dioxide through exhaling Air flue discharge.There are also researchs to report different results.After Barclay thinks that Glucosamine is oral, crosses and imitate by head Should be in conjunction with plasma protein, bioavilability 26%, unbonded aminoglucose saccharide part concentration at articular cartilage.In vitro Test prompt, when Glucosamine generates various effects, blood concentration need to be higher than 100mmolL-1.Biggee etc. is giving The Human Osteoarthritis Glucosamine 1500mg at 18 one nights of fasting, leaves and takes blood sample in certain time.The drug of blank blood sample Concentration is lower than 0.5mmolL-1, and maximum plasma concentration is 11.5mmolL-1 in test, well below obtaining in other researchs Concentration required for vivo efficacy.Thus the researchers such as Biggee produce query for the curative effect of Glucosamine, because this A little curative effects are built upon on the basis of the biological theory of current in vitro study middle and high concentration.Glucosamine is each for treating The Osteoarthritis at a position, such as the Osteoarthritis of knee joint, hip joint, swivel of hand.Since Glucosamine listing, Multinomial clinical research has been carried out to it both at home and abroad.Symptom improvement result：In 1994, Noack etc. was evaluation Glucosamine Effectiveness and reliability has carried out a multicenter, random, placebo research.252 outpatient service Human Osteoarthritis according to According to the evaluation of Li Kuisenshi (Lequesne) index, (it radiates I to III grade of histological grading, and Lequesnez index is at least 4 points, and extremely Rare 6 months or more symptoms), it is random to be grouped, Glucosamine 500mg (treatment group) or placebo (control group) are taken, often Day 3 times, medication 4 weeks.When studying beginning, every group of Lequesne index is (10.6 ± 0.45) point.It is treated at the end of research Group is down to (7.45 ± 0.5) point (averagely reducing 3.2 points), and placebo is (8.4 ± 0.4) point (averagely reducing 2.2 points) (P< 0.05, S examines).Treatment group and placebo effective percentage are respectively 55% (n=120) and 38% (n=121).Patient tolerability Good, 2 groups do not find notable difference.The studies have shown that Glucosamine is a kind of slow work of safe and effective treatment osteoarthritis Use drug.Structural improvement effect：2001, by randomized double-blind placebo controlled clinical trial, Reginster etc. studied amino Long-term influence of the glucose on osteoarthritis.212 knee Human Osteoarthritis are grouped at random, take 1500mg amino Portugal daily Grape sugar or placebo, medication 3 years.Front and back when every side knee is fully extended weight bearing is shot within 1 year and 3 years at pre-treatment and after treatment Position x-ray photo measures the mean breadth of shin, femoral joint intermediate cavity using the method for digital image analysis.Minimum articular cavity is wide Degree is the most narrow place of articular cavity, with amplification sem observation.Symptom score is carried out according to WOMAC.Result of study is shown, through X-ray film The progressive joint space of confirmation is averaged narrowing degree, is 0.31mm in placebo, and in Glucosamine group (1500mg D-1) without substantially changeing, only 0.06mm, two groups have significant difference.The patient of oral placebo, symptom ratio application amino Glucose curer slightly aggravates.Treatment group and placebo nothing in terms of the reason of test is exited in safety and early stage are obvious poor It is different.Result, it is believed that the long-term treatment of aminoglucose can prevent the development of knee OA.The Glucosamine that the present invention uses is from commercially available purchase It obtains and meets National Drug Administration's chemical drug terrestrial reference and rise the 16th contained standard criterion of page 226 of national standard.

Calcium carbonate is the most common form of human body supplement calcium agent.Calcium ion be body items physiological activity it is indispensable from Son.It maintains normal Nerve conduction for the biotic potential of maintenance cell membrane two sides.Maintain normal muscle flexible with Diastolic function and nerve-muscle conduction function are showed by calcium ion there are also some functions of hormones mechanism.Calcium Ion is existence form of the calcium constituent in compound.Calcium atom loses two electronics just at calcium ion.Calcium constituent (Ca) It is important one of human body composition, is generally always existed in the form of calcium ion.Calcium is very big in people's in-vivo content, and the overwhelming majority all exists In bone and tooth, minimal amount is present in blood and tissue.Since metabolism requires to supplement one from food daily Quantitative calcium.Adult recommends every 1000 milligrams of daily ingestion, recommends daily 1300 milligrams when growing up.So teenager needs Calcium it is higher than adult because the development of bone needs.Height growth is related with heredity, but also relates to the nutrition day after tomorrow. Guarantee that enough nutrition includes that calcium is critically important, this is also why present people is than parent's generation's average height.Various foods There is calcium ion in object, in milk and dairy produce at most.Such as 200 milliliters of milk (one glass or so) calcium ions, 300 milligrams of left sides It is right.So even if having no appetite eat that goodies are not plus two glasss of milk it is enough.A little beans systems are eaten if really mending more Product, the high food of the calcium contents such as spinach, natural food nutrition equilibrium are best selections.Calcium ion is body items physiological activity Indispensable ion.It maintains normal Nerve conduction for the biotic potential of maintenance cell membrane two sides.Remain normal Muscle it is flexible with diastolic function and nerve-muscle conduction function, pass through calcium ion there are also some functions of hormones mechanism It shows.Its main Physiological Function be all based on more than elementary cell function, mainly have the following：1. calcium ion is Coagulation factor participates in coagulation process；2. participating in muscle (including skeletal muscle, smooth muscle) contraction process；3. participating in neurotransmitter to close At with release, hormone sensitive lipase gene and secretion；4. being the important substance that bone is constituted.Wherein the generation mechanism of several important function is such as Under：Afferent nerve signal, mechanism：Promote neurotransmitter secretion.When first cell excitement, an electricity impulsion is produced, this When, extracellular calcium ion flows into this into the cell, promotes the cell secretory nerve mediator, neurotransmitter and adjacent next stage mind It is combined through the protein molecular on cell membrane, this level-one nerve cell is promoted to generate new electricity impulsion.And so on, nerve signal is just It hands on one by one, to constitute complicated signal scheme, or even the final advanced function for the brains such as study, memory occur Energy.When body calcium deficiency, the release of neurotransmitter is obstructed, and the excitation mechanism and suppression mechanism of human body are destroyed.If It is children's calcium deficiency, the meeting morbid night crying of babies, sleep terror fright at night, irritated insomnia are serious to lead to brain development obstacle, slow in reacting, more dynamic, study occur The diseases such as difficulty influence brain maturation and intelligence.Allow heartbeat, mechanism：Positively charged calcium ion allows intraor extracellular that current potential occurs Difference.Positively charged calcium ion passes through cell membrane, into cardiac muscle cell because the calcium concentration difference of intraor extracellular is larger, formed compared with Big potential difference produces the physiological effect that stimulation cell membrane is shunk.Mycardial contractility, and calcium ion has been pumped out into cell membrane Outside, reversed potential difference is formed, myocardial cell membrane starts diastole under the action of this reverse potential is poor；After diastole, cell membrane Permeability enhancing, calcium ion again passes through cell membrane and enters cardiac muscle cell, causes myocardial contraction, and so on, heart again It beats with just having the rhythm and pace of moving things.

Signal of resisting the enemy is transmitted, mechanism is：Exotic antigen activates T cell receptor, and the signal for starting calcium-mediated is logical Road promotes immune cell differentiation and growth.When the foreign invaders such as germ, bacterium, poisonous substance invade human body when, be calcium ion first It issues warning signal；Subsequent calcium ion issues the signal what characteristic invader has again, and immune system is organized immune accordingly therewith Cell, capture and phagocytosis enemy.Once calcium deficiency will occur function of immune system decline, disorder, cause disease.Such as：Itself Immunity disease lupus erythematosus, rheumatism；Skin disease：Dermatitis, acne etc..It replenishes the calcium, plays an important role, give counterevidence to these diseases are treated The function of calcium.The activity of enzyme is adjusted, mechanism is：One kind of intracellular calmodulin and calcium binding, formation is multiple Object is closed, the activity of internal a variety of enzymes can be activated.If skin is hurt, bleed, calcium ion issues signal at once, swashs step by step Fibrin ferment living, starts clotting mechanism, with hemostasis.Nutrition in food will lean on the decomposition of enzyme, can just be absorbed by the body, and albumen A variety of enzymes such as enzyme, lipase, amylase, ATP enzyme and hormone, will lean on the effect of calcium ion, can just be full of activity, therefore nutrition There is the saying of " replenishing the calcium, be the root for supplementing all nutrition ".Regulate and control the maturation and fertilization of reproduction cell, mechanism is：Sperm DNA Front end, be the acrosome being made of calcium.The front end for the DNA that sperm carries is the acrosome being made of calcium, exactly This calcium acrosome makes sperm when reaching egg cell edge, destroys and penetrate the inner layer film of egg cell, fertilization in a flash with regard to this Sample has occurred.For the waves against being made of simultaneously calcium around egg cell, this is referred to as calcium oscillation.Calcium oscillation plays activation ovum Effect, so that ovum is obtained fertility, life is bred from that point on.Therefore, if calcium is inadequate, people is directly affected Sexual function and sperm vigor, lead to infertility.

Recent studies have found that calcium participates in wider physiology course, such as the control of cell excitability, cell metabolism, thin Maintenance, regulation of cell cycle of born of the same parents' form etc..Calcium ion can be mainly manifested in as follows with the relevant enzyme of activation signal transduction Aspect：1, distribution of the calcium ion in cell has apparent provincial characteristics, and the free calcium concentration of extracellular fluid is much higher than cell Interior calcium concentration, and intracellular calcium ion then have it is 90% stored above in intracellular calcium store (in endoplasmic reticulum and mitochondria), cytoplasm Interior calcium concentration is very low.If the calcium channel of cytoplasma membrane or intracellular calcium store open, can cause extracellular calcium interior stream or The calcium of intracellular calcium store discharges, and increases calcium ion concentration in cytoplasm sharply.And after calcium ion enters cytoplasm, and can be again through cell Calcium pump (calcium ion-ATP enzyme) on plasma membrane and calcium library film returns to extracellular or intracellular calcium store, maintains intracytoplasmic low calcium State.2, the downstream signaling molecules of calcium ion are calmodulins, and calmodulin (calmodulin, CaM) is a kind of calcium knot Hop protein has 4 structural domains in molecule, and each structural domain is in combination with 1 calcium ion.When calcium ion concentration is low in cytoplasm, calcium tune Albumen is not easy to combine calcium ion；As calcium ion concentration increases in cytoplasm, calcium ion of the calmodulin in combination with different number, shape At the calcium ion/calmodulin compound of different conformations.Calmodulin itself is inactive, and it is compound to form calcium ion/calmodulin There is regulatory function after object, the activity of calmodulin-dependent protein kinase is adjusted.3, calmodulin be not calcium ion only One target molecule, in addition to calmodulin, calcium ion passes through change herein in connection with the multi-signals transduction molecule such as PKC, AC and cAMP-PDE Structure effect activates these molecules

Chondroitin sulfate (CHONDROITIN SULFATE, CS) is covalently attached the formation proteoglycans on protein A kind of glycosaminoglycan.Chondroitin sulfate is distributed widely in the extracellular matrix and cell surface of animal tissue, and sugar chain is by alternate Glucuronic acid and N- acetylgalactosamine (also known as N- acetylamino galactosamine) disaccharide unit composition, are connect by one like sugar chain Area is connected on the serine residue of core protein.Chondroitin sulfate is present in from nematode to people except exophytic all biologies In, play many important physiological functions.Although the backbone structure of polysaccharide is simultaneously uncomplicated, with regard to degree, sulfate For the distribution into isomery uronic acid again chain, the inhomogeneity of height is presented with two species diversity.The fine knot of chondroitin sulfate Structure decides the specificity of function and the interaction with multiple proteins molecule.1956, Meyer etc. was first begin to difference The research of the type and content of acidic mucopolysaccharide in tissue, identifies in connective tissue that there are CS and hyaluronic acid, sulfuric acid angle The glutinous polysaccharide such as quality.The unsaturation sugar that CS is generated under acid, alkalinity and enzymatic hydrolysis condition, oligosaccharides including low molecule CS and CS or Disaccharide is related with beta-elimination reaction.CS acid, alkalinity and with the palliating degradation degree under neutrallty condition with the ultraviolet suction at 232nm Shading value indicates that ultraviolet light absorption angle value is bigger, and expression palliating degradation degree is bigger, to reflect the stability of CS at different conditions. CS is the acid mucopolysaccharides that preparation is extracted from animal tissue, for white or off-white powder；It is odorless；Have draw it is moist. The aqueous solution of this product has toughness, heats non-condensing.This product is readily soluble in water, organic molten insoluble in ethyl alcohol, acetone and ether etc. In agent, its esters is more stable to heat, heated not also to be destroyed up to 80 DEG C.Chondroitin sulfate aqueous solution, meets higher temperature or acid is It is unstable, mainly deacetylation or it is degraded into monosaccharide or the lesser polysaccharide of molecular weight.Chondroitin sulfate is extensively medically main Application approach be as treatment joint disease drug, be used cooperatively with Glucosamine, have analgesic, promote regenerating bone or cartilage The effect of, joint prob can be improved from basic.The clinical test of random placebo control is it has been proved that chondroitin sulfate can Human Osteoarthritis pain is reduced, improves function of joint, reduce arthroncus and hydrops and prevent knee joint and swivel of hand position Narrow gaps.Pad effect is provided, the impact and friction when action are mitigated, moisture proteoglycan intramolecular can be sucked, made soft Bone thickens, and increases intra-articular synovia amount.One of critical function of chondroitin is exactly that important oxygen is supplied as conveyance conduit It is delivered to joint with nutrient, intra-articular waste is removed in help, while carbon dioxide and waste are excluded.Due to joint Cartilage has no blood supply, therefore all oxygenation, nourishing and lubricating actions all come from synovia.Chondroitin sulfate is to corneal collagen Fiber has protective effect, can promote the growth of fiber in matrix, enhances permeability, improves blood circulation, accelerates metabolism, Promote the absorption of penetrating fluid and the elimination of inflammation；Its polyanion has strong water-retaining property, can improve the moisture of corneal tissue Metabolism, has stronger affinity to cornea, can form one layer of ventilative water-retaining film in anterior corneal surface, improve eye dryness symptom.It is logical The generation for promoting matrix is crossed, framework is provided for the migration of cell, is conducive to the migration of corneal epithelial cell, so that cornea be promoted to create The healing of wound, the absorption of diffusate and the elimination of inflammation.By high-tech deep processing, nervous headache, trident mind can treat The diseases such as dysmenorrhoea, coronary heart diseases and angina pectoris, myocardial anoxia, cardiovascular and cerebrovascular disease, arthralgia, atherosclerosis and hepatitis, wherein CS Also there is anticoagulant and antithrombotic effect, it can also be used to auxiliary because of dysacousis caused by streptomysin and compromised liver function Help the adjuvant treatment for the treatment of and hyperlipidemia.It can be used as health care product, the additive in food, there is enhancing human body constitution and anti- The effects of germ, beauty, anti-aging.Improve hearing and dry skin.It can inhibit suction of the small intestine to lipid and glucose in vivo It receives to reach antiobesity action.

Chondroitin sulfate is widely present in humans and animals cartilaginous tissue.Its pharmaceutical formulation mainly contains chondroitin sulfate A (CSA) With two kinds of isomers of chondroitin sulfate C, the content of chondroitin sulfate is different in the cartilage of the animals such as different cultivars, age.Its medicine Reason effect is shown as：1, CS can remove the lipid and lipoprotein in internal blood, remove the cholesterol of heart, prevent Atherosclerosis is controlled, and increases the conversion ratio of lipid and fatty acid in the cell.2, CS can effectively prevent coronary heart disease.To reality The property tested artery sclerosis model has antiatherosclerosis and anti-cause atherosclerosis plaque forming effect；Increase the hat of atherosclerosis Shape artery divides skill or Doppler flow mapping, and can myocardial necrosis or denaturation caused by Acceleration study coronary sclerosis or embolism Healing, regeneration and reparation.3, it can increase the biosynthesis of the messenger RNA (mRNA) and DNA (DNA) of cell And have the function of promoting cell metabolism.4, anticoagulant active is low.Chondroitin sulfate has the blood coagulation resisting function mitigated, often 1mg chondroitin sulfate A (CSA) is equivalent to the anticoagulating active of 0.45U heparin.This anticoagulating active is not rely on Antithrombin III and sends out The effect of waving, it can play anticoagulant active by fibrin original system.5, chondroitin sulfate also has anti-inflammatory, accelerates wound Mouthful healing and antitumor etc. effect.The long-term clinical application discovery of chondroitin sulfate, deposits on artery and wall of vein The lipids such as fat can be removed efficiently or reduce, can significantly reduce plasma cholesterol, thus atherosclerosis Formation.Chondroitin sulfate is for treating neuralgia, nervous migraine, arthralgia, arthritis and omoplate arthralgia, abdominal cavity Postoperative pain etc..Prevent and treat hypoacusia, tinnitus disease caused by dysacousis caused by streptomysin and various noises Deng significant effect.There is auxiliary therapeutic action to chronic nephritis, chronic hepatitis, keratitis and ulcer of the cornea etc..In Shark cartilage Cartilage be known as antineoplastic action.In addition, chondroitin sulfate is also applied to the consolidant etc. of cosmetics and trauma wounds.Sulphur Aching and limp ossein is a kind of acid mucopolysaccharide, is one of the important composition in ocular tissue, has and promotes cornea water metabolism and improvement, Suitable for visual fatigue, xerophthalmia.Chondroitin sulfate can effectively improve bone mass, by the confession for increasing chondroitin sulfate It answers, body itself adjustment mechanism is inhibited, and bone sclerosis is reduced, and the flexible bone of consolidation is reduced than fragile bone, volume 10%, intensity greatly enhances.Especially face contour is not easy to deform or expand aggravation, and bioadhesion improves.Profile bone Bone journey reduces trend, restores younger state.When taking gucosamine also and meanwhile absorb chondroitin be it is extremely important, because of cartilage Element can promote the process in gucosamine infiltration joint.Multinomial studies have shown that includes that a wherein publication is miscellaneous in New England's medicine The CINEFL research institution of will points out, can more effectively protect in conjunction with intake gucosamine and chondroitin, reverse damage and promote Repairing articular cartilage and membrane bone allow bone that younger state is presented so that osteosclerosis is reduced.It is also same when taking gucosamine When intake chondroitin be it is extremely important because chondroitin can promote gucosamine penetrate into joint process.Chondroitin sulfate can also Enough ferment (such as clostridiopetidase A, elastoser and cathepsin) for inhibiting to destroy cartilage, in case cartilage is decomposed or dissolves, Or hardening.It can set about from the root of problem, inhibit the activity of COX-2, to prevent Joint Inflammation.

Casein phosphopeptide (CaseinPhosphopeptides, abbreviation CPP) is passed through using bovine casein as raw material The polypeptide of biotechnology tool bioactivity obtained.CPP molecule is made of 20 to thirties amino acid residues, including Phosphoric acid silk acyl group existing for 4~7 clusters.A large number of experiments proves that CPP can be effectively facilitated human body to the divalent mine such as calcium, iron, zinc The absorption and utilization of object nutrient.Casein phosphopeptide can be used for various nutrition, in health food, can effectively facilitate human body pair The absorption and utilization of the divalent minerals nutrient such as calcium, iron, zinc.Casein phosphopeptide is the junket egg with pancreatin or trypsin hydrolysis It is white, it is made by refining, purifying, nuclear structure is：—Ser(P)-Ser(P)-Ser(P)-Glu-Glu-(Ser：Silk Propylhomoserin, Glu：Glutamic acid, P：Phosphate).Phosphoserine residue (- Ser (P) -) cluster in this structure exists, in intestines It is negatively charged under road PH weakly alkaline environment, the further effect of digestive ferment can be prevented, hydrolyze CPP will not further and in intestines In be stabilized.Studies in China finds that the molar ratio of nitrogen and phosphorus is smaller in CPP, and the peptide chain of CPP is shorter, the density of phosphate Bigger, then CPP purity is higher, promotes the absorption of calcium and utilizes effect also stronger.Calcium is only with just easy in the presence of ionic forms It is absorbed, and is easy to form insoluble salt with acid ion and be lost again in neutral and weakly alkaline environment.Suction of the CPP to calcium Adduction is mainly shown as, can be inhibited the generation of infusible precipitate in conjunction with calcium under neutral and weakly alkaline environment, be avoided calcium It is lost, finally the Passive intake due to raising of free calcium concentration.Current study show that the effect that CPP promotees calcium uptake mainly shows In the following aspects：Promote absorption of the small intestine to calcium, the cereal in the diet of people contains a large amount of phytic acid, six phosphorus of inositol The contour phosphorus composition of acid, generates calcium phosphate precipitation in conjunction with calcium under the environment of PH7~8 of small intestine lower end.And CPP can inhibit calcium phosphate The formation of precipitating makes free calcium keep higher concentration, promotes the Passive intake of calcium, becomes vitamin D and promotes as calcium uptake The another approach of agent.Promote utilization of the bone to calcium, animal experiments show that, CPP can promote the absorption and utilization of calcium, weaken osteoclastic Cytosis and the re-absorption for inhibiting bone.Promote utilization of the tooth to calcium, it is considered that, it is postprandial to chew cheese energy saliva stimulating point It secretes, makes corrosion of the acidic materials on the Saliva buffer dental plaque of alkalinity to enamel, facilitate the generation for preventing saprodontia.In recent years it grinds Studying carefully discovery, the CPP contained in cheese can mitigate enamel and go to mineralize by the calcium binding in food at saprodontia, thus Reach anti-caries purpose.The study found that the sperm in the culture solution containing CPP, hence it is evident that have the higher energy for penetrating egg cell Power, moreover it is possible to reduce the degree of variation of sperm and keep embryonic development more stable.CPP can also improve the metal ions such as iron, zinc, magnesium Bioavilability, thus it is referred to as the peptide matters with metallic carrier function.Currently, external be applied to children's coffee for CPP In the food and health care product such as curry meal, beverage, chewing gum.To children's calcium deficiency, senile osteoporosis, the treatment of sterility and tooth Research and application in terms of health care is also in process.Because CPP is the polypeptide extracted from native protein, have bad anti- Small, safe and reliable advantage is answered, thus will be more widely used.Foreign study casein, defatted milk protein and CPP Sensitivity response, find the sensitization very little of CPP, show that it can be suitable for anti-milk constitution.But it should also be mentioned that The factor for influencing CPP effect is extremely complex, and effect during calcium metabolism must also carry out in-depth study.Casein phosphopeptide PH is in neutrality in weakly alkaline environment in human body intestinal canal, and CPP can chelate calcium, iron, zinc ion, is protected it from by diet The anion such as phosphoric acid, oxalic acid, phytic acid precipitating, to effectively facilitate the absorption and utilization to calcium, iron, zinc etc..CPP also exactly has in mind In the novel product for improving being absorbed and utilized for calcium and developing, CPP has following spy compared with traditional calcium enhancer vitamin D Point：1.CPP may additionally facilitate the absorption of the divalent minerals nutrient such as iron, zinc other than promoting calcium uptake function.Iron and zinc be in addition to The minerals that Chinese most easily lack other than calcium can affect to the absorption of iron and zinc if merely, excessively replenishing the calcium. If CPP is made an addition in the nutraceutical or nutrient and healthcare products for strengthening calcium, iron, zinc, the absorption of calcium not only can be improved, simultaneously also The absorption rate of iron and zinc can be improved, thus effectively overcome general calcium supplement to the potential for adverse effects of iron, zinc-iron alloy solution, this Selecting is characteristic that any other calcium supplement does not have.2. the active transport that vitamin D can promote the saturable calcium of upper part of small intestine It absorbs, the effect of vitamin D is influenced by age and calcium intake；To small intestine lower part, saturable Passive diffusion does not absorb CPP, this Kind, which absorbs, not to be influenced by age and calcium intake variation, and the Passive diffusion of calcium is absorbed much larger than active transport absorption.CPP is produced Product are also with good stability, and the shelf-life was up to 18 months or more under the conditions of dry be protected from light.After being added in product, in acid Good stability is all had in property to neutral range, is resistant to the high-temperature process of 120 degree, 30 minutes.Under alkaline condition, stablize Property it is poor, improved with heating temperature and heated time extend, dephosphorization acid-base reaction can aggravate, and the function of CPP will receive influence.? Under average operating, the structure and function of CPP is all stable.3. the absorption of calcium, but VD can be increased by strengthening VD in diet Excess intake can cause certain harm to kidney and bone.And if while reinforcing calcium, adding a certain amount of CPP, then in diet The calcium in enteron aisle can be made to be maintained at dissolved state, to effectively facilitate the absorption and utilization of calcium, and CPP derives from cow's milk protein Matter will not generate any toxic side effect to human body excess is taken.In addition, CPP has within the scope of very wide pH completely The characteristic of dissolution can tolerate high-temperature process, with good stability, when CPP makes an addition to food, have low amounts efficiently special Point, the product for adding CPP can keep original flavor and taste.The calcium binding capacity of CPP is maintained under certain condition by CPP The size of calcium amount in solution.In having solution system existing for calcium ion and phosphate radical, when pH is in alkalinity, there will be calcium phosphate to sink It forms sediment and is formed.The presence of CPP can reduce the calcium because of caused by calcium phosphate precipitation and lose, and more calcium is made to keep dissolved state.Ca：P =1：1(8mM：8Mm) and 2：1(12Mm：When 6Mm), the CPP of 200ppm is added in system, keeps the temperature 1 hour at 35 DEG C.Ca：P =1：1 and 2：When 1, CPP's holds calcium amount (indicating with every mole in the CPP molecule organic phosphorus molal quantity for keeping dissolving calcium) respectively It is 37.2 and 28.0.In terms of CPP prevents osteoporosis, animal model used is big for ovariectomized old female in animal experiment Mouse.It is test group that CPP is added in feed, and series 2 is not give the control group of CPP in feed.When test was carried out to 4 months, The opposite bone density of test group has been significantly higher than control group, and it is good to illustrate that CPP has in the bone loss for preventing old female rats Good effect.CPP and calcium have the combination of appropriateness, and such bond strength can protect calcium ion not to be deposited, and be enough to push away It moves a large amount of dissolving calcium to transport to intestinal mucosa, influences the absorption of calcium without regard to because combining too closely.The combination of CPP and calcium Be it is dynamic, calcium ion constantly combines by CPP, release, in conjunction with, again discharge so that a phosphate can in CPP molecule To protect more than 30 a calcium ions not to be deposited.

Scientific research proves that under normal circumstances, the absorptivity of various calcium sources is very nearly the same.Why milk is many institute's weeks Know and the favorite supplementary calcium food of the mankind, reason is other than milk calcic is relatively abundant, also because milk zymoprotein is enteral in vivo Road albumen enzyme effect produces part CPP, and CPP can promote the absorption and utilization of calcium, and in other words, absorptivity should not become producer The foundation for selecting calcium source, suitable accelerating agent of calcium absorption CPP is added in the product and is only wise move.CPP is by milk The product that casein is extracted through biotechnology is qualified " crude milk essence ", therefore CPP is added in calcium supplementing product It is most suitable.

CPP has the characteristic being completely dissolved within the scope of very wide pH, can tolerate high-temperature process, has good stabilization Property, therefore, it can make an addition in following various products：The nutrient and healthcare products milk fish for strengthening calcium, iron, zinc, such as fruit milk are learned The child nutrition foods such as raw formula milk, high-calcium low-fat milk, such as Mutritive infant rice flour, high calcium biscuit bean product, such as high calcium The carbonated beverages such as the toothpaste beer of the preventing decayed tooths permanent tooths such as nutrient oatmeals, the chewing gums such as soymilk powder, calcium bean curd, light sparkling wine (can make Foam is fine and smooth, persistently, and promotes mineral absorption therein) in the said goods in application, CPP, which can be played, makes product formula more It is perfect, reasonable, it is really achieved the purpose that these mineral nutrients are supplemented to human body, while the upgrading that can also be achieved existing product is changed Generation.In addition, the product of addition CPP, original flavor and taste can remain unchanged.The said goods are to strengthen calcium, iron It is added to CPP in product, makes it have the characteristic for being different from other similar products in the market, is in market leading position.CPP is not The absorption that only can promote calcium has good facilitation effect to iron, being absorbed and utilized also for zinc.Therefore the nutrition food of exploitation addition CPP Product and health care product can be really achieved the purpose for the minerals that effectively supplement human body lacks, meet the nutritional need of people, must can produce Raw huge economic benefit and social benefit.Casein phosphopeptide derives from natural good protein --- bovine casein, it can To effectively improve the intake and absorption and utilization rate of the divalent minerals such as human calcium, iron, zinc, also there is permanent tooth, strong tooth, repair The effect of multiple gum tooth.CPP is also exactly to be conceived to the absorption rate for improving calcium and the novel nourishing product developed, it is to be at present One of the biology peptide type food additives living of industrialized production are only realized, absorptivity is high compared with VD for it, has no toxic side effect, more The absorptivity of the divalent minerals such as other zinc, iron can be improved.CPP can promote small intestine lower part not saturable calcium Passive diffusion inhale It receives, this absorption is not by the variation at age and VD.

In the present compositions, it has had now surprisingly been found that, a small amount of behenyl is added together with chondroitin sulfate Acid glyceride can significantly improve the stability of wherein important activity ingredient Puerarin, and it is found through experiment that Puerarin stabilization Property is influenced by chondroitin sulfate.This specific method for improving Puerarin stability and result are as follows：Respectively refer to reality 1~embodiment of example 5 is applied, different is only in step (1), and Compritol 888 ATO is also added with chondroitin sulfate, and (each example adds Dosage be respectively equivalent to chondroitin sulfate weight 20%, 25%, 15%, 18%, 22%), obtaining 5 batches can be referred to as to mend Fill an a1~supplement example a5 tablet；1~embodiment of embodiment, 5 tablet and supplement example a1~supplement example a5 tablet is set to be placed in 40 Up to 6 months at DEG C temperature, version in 2015 is used《Chinese Pharmacopoeia》One contained pueraria lobata of page 333【Assay】Method measurement is each Criticize puerarin content of the tablet at 0 month and when June in tablet；Its Puerarin remnants hundred are calculated as follows for each sample Score：Puerarin remnants percentage=June 0 month puerarin content × 100% of puerarin content ÷；As a result, embodiment 1~implementation The Puerarin remnants percentage of 5 tablet of example is 90.6%~92.2%, and supplement example a1~supplement example a5 tablet Puerarin is remaining Percentage is 98.8%~99.6%, and the latter, which shows, is higher than the former, shows the important physiological activator pueraria lobata in the latter's tablet It is plain obvious more stable；In addition, respectively referring to 1~embodiment of embodiment 5, different is only that chondroitin sulfate is not added in prescription, Obtaining 5 batches can be referred to as to supplement example b1~supplement example b5 tablet, so that this five batches of tablets is carried out 40 DEG C -6 with above method Month disposition, Puerarin remnants percentage when measuring and calculating June, as a result their Puerarin remnants percentage be 98.1%~ 99.3%.It is adverse effect by chondroitin sulfate that this, which shows that puerarin content in 1~embodiment of embodiment 5 is substantially reduced, and This adverse effect can be overcome by adding this field conventional pharmaceutical adjuvants Compritol 888 ATO.Therefore, appoint according to the present invention On the one hand, wherein also adding Compritol 888 ATO.In one embodiment, wherein the additive amount of Compritol 888 ATO is equivalent to The 15%~25% of chondroitin sulfate weight.In one embodiment, Compritol 888 ATO is added together with chondroitin sulfate Add.

Of the invention is used to improve bone density for the middle-aged and the old, improves osteoarticular function, avoids or reduces waist-leg cramp Chinese medicine composition is made of kudzu root extract, aminoglucose hydrochloride, calcium carbonate, chondroitin sulfate, casein phosphopeptide.It is in The combination biological effectiveness of these existing medicinal materials especially has the bone density that increases significantly, improves osteoarticular function, avoids or subtract The effect of few waist-leg knots.For example, being directed to low 36 people of subject of bone density, give 1 gained of the embodiment of the present invention for each person every day Composition 10g, point takes twice sooner or later, and after 2 months, the bone density of whole subjects improves 23% or more up to 23.3%~ 27.6% range.This shows that group symphysis of the invention has the effect of excellent raising bone density, these subjects were at 2 months Feedback osteoarticular function has clear improvement afterwards.In another example for 48 people of subject for having 3 times or more generation leg cramps in nearly January, Give 1 resulting composition 10g of the embodiment of the present invention for each person every day, a point morning and evening takes twice, persistently takes 2 weeks, 2 weeks later 2 In a month, whole subjects do not occur leg cramps.

In addition, based on the present invention for improving bone density for the middle-aged and the old, improving osteoarticular function, avoid or reduce waist-leg The physiologic properties of various medicinal materials in the Chinese medicine composition of cramp, completely it is expected that the present invention is used to improve for the middle-aged and the old These medicinal materials can be presented respectively or group in bone density, the Chinese medicine composition for improving osteoarticular function, avoiding or reducing waist-leg cramp Close the biological effect generated.Such as the present invention is for improving bone density for the middle-aged and the old, improvement osteoarticular function, avoiding or subtract The Chinese medicine composition that few waist-leg knots can be endowed pueraria lobata, aminoglucose hydrochloride, calcium carbonate, chondroitin sulfate, casein Phosphoeptide respective canonical biometric effect as described above.Therefore, the purposes of third aspect present invention further include it is above-mentioned these Purposes involved in treatment/preventive effect.

Specific embodiment

The present invention can be further described by the following examples, however, the scope of the present invention and unlimited In following embodiments.One of skill in the art, can be with it is understood that under the premise of without departing substantially from the spirit and scope of the present invention Various change and modification are carried out to the present invention.The present invention carries out the material and test method arrived used in test general And/or specific description.Although to realize the present invention many materials and operating method used in purpose be it is known in the art that But the present invention is still described in this detail as much as possible.

Every batch of total amount of feeding is 10 kilograms in following compositions.

Embodiment 1：Prepare Chinese medicine composition

Formula：500 parts by weight of kudzu root extract, 440 parts by weight of aminoglucose hydrochloride, 400 parts by weight of calcium carbonate, sulfuric acid 100 parts by weight of chondroitin, 25 parts by weight of casein phosphopeptide, 760 parts by weight of xylitol, 250 parts by weight of microcrystalline cellulose, tristearin Sour 25 parts by weight of magnesium.

Preparation method：

(2) so that casein phosphopeptide is added the water dissolution of 6 times of amounts, add 2.5 times of casein phosphopeptide weight of xylitol, It is uniformly mixed, makes this mixture ventilation process 2.75 hours at a temperature of 65 DEG C, be uniformly mixed；

(4) it is uniformly mixed dry particle obtained by previous step with magnesium stearate, obtains composition, be further pressed into Tablet, every 2.5 grams, be chewable tablets.

Embodiment 2：Prepare Chinese medicine composition

Formula：500 parts by weight of kudzu root extract, 400 parts by weight of aminoglucose hydrochloride, 500 parts by weight of calcium carbonate, sulfuric acid 50 parts by weight of chondroitin, 40 parts by weight of casein phosphopeptide, 600 parts by weight of xylitol, 280 parts by weight of microcrystalline cellulose, tristearin Sour 22 parts by weight of magnesium.

Preparation method：

(2) so that casein phosphopeptide is added the water dissolution of 7 times of amounts, add 2 times of casein phosphopeptide weight of xylitol, mix It closes uniformly, makes this mixture ventilation process 2.75 hours at a temperature of 65 DEG C, be uniformly mixed；

Embodiment 3：Prepare Chinese medicine composition

Formula：500 parts by weight of kudzu root extract, 500 parts by weight of aminoglucose hydrochloride, 300 parts by weight of calcium carbonate, sulfuric acid 150 parts by weight of chondroitin, 10 parts by weight of casein phosphopeptide, 900 parts by weight of xylitol, 220 parts by weight of microcrystalline cellulose, tristearin Sour 28 parts by weight of magnesium.

Preparation method：

(2) so that casein phosphopeptide is added the water dissolution of 8 times of amounts, add 3 times of casein phosphopeptide weight of xylitol, mix It closes uniformly, makes this mixture ventilation process 3 hours at a temperature of 60 DEG C, be uniformly mixed；

Embodiment 4：Prepare Chinese medicine composition

Formula：500 parts by weight of kudzu root extract, 470 parts by weight of aminoglucose hydrochloride, 350 parts by weight of calcium carbonate, sulfuric acid 125 parts by weight of chondroitin, 20 parts by weight of casein phosphopeptide, 800 parts by weight of xylitol, 240 parts by weight of microcrystalline cellulose, tristearin Sour 26 parts by weight of magnesium.

Preparation method：

(2) so that casein phosphopeptide is added the water dissolution of 5 times of amounts, add 2.5 times of casein phosphopeptide weight of xylitol, It is uniformly mixed, makes this mixture ventilation process 2.5 hours at a temperature of 70 DEG C, be uniformly mixed；

Embodiment 5：Prepare Chinese medicine composition

Formula：500 parts by weight of kudzu root extract, 420 parts by weight of aminoglucose hydrochloride, 450 parts by weight of calcium carbonate, sulfuric acid 75 parts by weight of chondroitin, 30 parts by weight of casein phosphopeptide, 700 parts by weight of xylitol, 260 parts by weight of microcrystalline cellulose, tristearin Sour 24 parts by weight of magnesium.

Preparation method：

(2) so that casein phosphopeptide is added the water dissolution of 6 times of amounts, add 2.3 times of casein phosphopeptide weight of xylitol, It is uniformly mixed, makes this mixture ventilation process 2.5 hours at a temperature of 65 DEG C, be uniformly mixed；

It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims Variation is included within the present invention.

Claims

1. the composition for avoiding waist-leg from knotting for improving middle-aged and the old's bone density and osteoarticular function comprising：Pueraria lobata extracts Object, aminoglucose hydrochloride, calcium carbonate, chondroitin sulfate, casein phosphopeptide and the acceptable load of optional physiology Body.

2. composition according to claim 1 comprising 500 parts by weight of kudzu root extract, aminoglucose hydrochloride 400~500 Parts by weight, 300~500 parts by weight of calcium carbonate, 50~150 parts by weight of chondroitin sulfate, 10~40 parts by weight of casein phosphopeptide, And the optional acceptable carrier of physiology.

3. composition according to claim 1 comprising 500 parts by weight of kudzu root extract, aminoglucose hydrochloride 400~500 Parts by weight, 300~500 parts by weight of calcium carbonate, 50~150 parts by weight of chondroitin sulfate, 10~40 parts by weight of casein phosphopeptide, And the optional acceptable carrier of physiology.

4. composition according to claim 1 comprising 500 parts by weight of kudzu root extract, aminoglucose hydrochloride 420~470 Parts by weight, 350~450 parts by weight of calcium carbonate, 75~125 parts by weight of chondroitin sulfate, 20~30 parts by weight of casein phosphopeptide, And the optional acceptable carrier of physiology.

5. composition according to claim 1 comprising 500 parts by weight of kudzu root extract, 440 weight of aminoglucose hydrochloride Part, 400 parts by weight of calcium carbonate, 100 parts by weight of chondroitin sulfate, 25 parts by weight of casein phosphopeptide and optional physiology Acceptable carrier.

6. composition according to claim 1, in the form of tablet or granule；Alternatively, it is the tablet form of chewable tablets.

7. composition according to claim 1, it is characterised in that：

In the form of tablet, the acceptable carrier of physiology includes xylitol, microcrystalline cellulose, magnesium stearate for it；

It is in the form of tablet, and in terms of every 500 parts by weight kudzu root extract, the amount of xylitol is 500~1000 parts by weight, such as 600~900 parts by weight, such as 700~800 parts by weight, such as 760 parts by weight；

It is in the form of tablet, and in terms of every 500 parts by weight kudzu root extract, the amount of microcrystalline cellulose is 200~300 parts by weight, Such as 220~280 parts by weight, such as 240~260 parts by weight, such as 250 parts by weight；Or

It is in the form of tablet, and in terms of every 500 parts by weight kudzu root extract, the amount of magnesium stearate is 20~30 parts by weight, such as 22~28 parts by weight, such as 24~26 parts by weight, such as 25 parts by weight.

8. composition according to claim 1 is prepared according to the method included the following steps：

(1) it is uniformly mixed kudzu root extract with aminoglucose hydrochloride, is separately uniformly mixed calcium carbonate and chondroitin sulfate；

(2) so that casein phosphopeptide is added the water dissolution of 5~8 times of amounts, add 2~3 times of casein phosphopeptide weight of xylitol, It is uniformly mixed, makes this mixture ventilation process 2.5~3 hours at a temperature of 60~70 DEG C, be uniformly mixed；

(3) various mixtures obtained by both the above step are mixed, add the xylitol of microcrystalline cellulose and surplus thereto, It is uniformly mixed, adding water is lubricant, prepares wet granular, keeps wet granular dry；

(4) it is uniformly mixed dry particle obtained by previous step with magnesium stearate, composition is obtained, as granular preparation shape Formula is directly packed, or is further pressed into tablet, obtains the dosage form of granule or tablet.

9. the method for preparing the composition of any one of claim 1~8, includes the following steps：

10. the composition of any one of claim 1~8 preparation for improve middle-aged and the old's bone density, osteoarticular function and The purposes in product for avoiding waist-leg from knotting.