CN108815205A - Cordyceps militaris extract and the preparation method and application thereof - Google Patents
Cordyceps militaris extract and the preparation method and application thereof Download PDFInfo
- Publication number
- CN108815205A CN108815205A CN201811073804.9A CN201811073804A CN108815205A CN 108815205 A CN108815205 A CN 108815205A CN 201811073804 A CN201811073804 A CN 201811073804A CN 108815205 A CN108815205 A CN 108815205A
- Authority
- CN
- China
- Prior art keywords
- cordyceps militaris
- preparation
- extract
- ethanol
- vacuum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of Cordyceps militaris extracts and the preparation method and application thereof.Present invention discover that the extract that Cordyceps militaris raw material is extracted by specific process can be further developed as the protection drug of acute kidney injury, have broad application prospects separately as the drug for the treatment of acute kidney injury.Meanwhile the preparation method of Cordyceps militaris extract of the invention is simple, is suitble to industrialized production.
Description
Technical field
The present invention relates to effective component extractive technique fields, and in particular to a kind of Cordyceps militaris extract and preparation method thereof with
Using.
Background technique
Acute kidney injury (acute kidey injury, AKI) is the more typical emergency and severe disease of clinical departments.AKI refers to 48h
Inside the raised absolute value >=26.5umol/L (0.3mg/dL) of at least 2 serum creatinine levels or more former serum creatinine value rise >=
50%；And (or) continue 6h or more urine volume<0.5mL/kg/h.Mostly occur in shock, severe trauma, septicopyemia and certain anti-swollen
It, many times can be fast because of progression of the disease after tumor (platinum class), antibiotic medicine (aminoglycoside, beta-lactam class) such as use at the factors
Speed, it is chronic kidney disease (CKD) so that relying on renal replacement therapies all the life even results in death that treatment develops not in time.
Domestic and international result of study shows that the disease incidence of AKI in general hospital is 3%~10%, intensive care unit be 30%~
60%, critical AKI mortality is up to 30%~80%.As long as Recent study shows that AKI occurs, can not only obviously increase
The short-term death rate of patient, and no matter whether renal function restores, all may be (including long term survival rate, chronic to the prognosis of patient's long term
The disease incidence of kidney trouble) generate adverse effect.In consideration of it, the treatment for AKI is just particularly important.
Cordyceps militaris also known as northern Chinese caterpillar Fungus, Cordceps militaris etc. belong to Clavicipitaceae, Cordyceps on taxology, be traditional simply
Chinese medicine has huge medical value.Contain in Cordyceps militaris there are many chemical component, there are a variety of medicinal efficacies, with cordyceps sinensis
Pharmacological function it is similar with clinical effectiveness, but price is lower than cordyceps sinensis, therefore gradually ingredient cordyceps sinensis replaces Cordyceps militaris
Dai Pin has great potential market.
Cordyceps militaris extract has multiple functions, for example, Cordyceps sinensis polysaccharide has antitumor, hypoglycemic, anti-hepatic fibrosis and mentions
High immune function, cordycepin have antibacterial, and anti-inflammatory, anti-oxidant, adjusting human endocrine and enhancing immune function of human body etc. are significant
Effect.But the also not report about Cordyceps militaris extract in terms for the treatment of acute kidney injury at present.
Summary of the invention
For the above-mentioned prior art, the object of the present invention is to provide a kind of Cordyceps militaris extracts and preparation method thereof.This hair
The Cordyceps militaris extract of bright preparation has significant therapeutic effect to acute kidney injury.
To achieve the above object, the present invention adopts the following technical scheme that：
The first aspect of the present invention provides a kind of preparation method of Cordyceps militaris extract, includes the following steps：
(1) it is 10-20% by Cordyceps militaris raw material drying to water content, then carries out vacuum and low temperature expanding treatment；
(2) ethanol solution is added in the Cordyceps militaris raw material after vacuum and low temperature expanding treatment and carries out ultrasonic extraction, extracting solution is dense
Contracting, drying, obtain crude extract；
(3) crude extract is redissolved with the ethanol solution that volumetric concentration is 40-60%, is adsorbed by macroporous resin column, successively
Through gradient concentration ethanol solution elution from low to high, 80% ethanol eluate is collected, solvent is removed, is concentrated into medicinal extract, then
Petroleum ether dissolution, filtering is added, filtrate removes solvent, and concentration, drying obtain Cordyceps militaris extract.
Preferably, in step (1), the Cordyceps militaris raw material includes：Cordyceps militaris, fruiting bodies of cordyceps militaris or Cordyceps militaris mycelia
Body.
Preferably, in step (1), the condition of the vacuum and low temperature expanding treatment is：45-55 DEG C of swelling temperature；Extraction is true
Empty number is 6-10 times, and puffing time is 1-2 hours.Vacuum and low temperature extruding is that sample is placed in pressurized tank, by heating and
Pressurization balances sample interior pressure and external pressure, and then vacuum is put in decompression suddenly, vaporize material internal moisture suddenly,
Flash distillation, makes sample cell extruding, by pumping repeatedly, puts vacuum processing sample interior cell tissue gap can be made to become and increase,
Permeability improves, it is easier to and Extraction solvent is in contact, to improve the recovery rate of effective component.And vacuum and low temperature extruding
Processing temperature is low, the time is short, substantially will not be to the active principle destroyed in raw material.
The research of the invention finds that the too low Cordyceps militaris of water content lacks flexibility during vacuum and low temperature expanding treatment,
It is frangible in pressure process；And moisture content it is excessively high when will affect the expansion effect of Cordyceps militaris.It is found through test of many times, Cordyceps militaris is former
Expect that expansion effect is ideal when water content is 10-20%.
Preferably, in step (2), the volumetric concentration of the ethanol solution is 50-60%.
Preferably, in step (2), the condition of the ultrasonic extraction is：40-60 DEG C of temperature, time 20-30min, power
100-120W。
Preferably, in step (3), the macroporous resin column is D101 type macroporous resin column.
Preferably, in step (3), the gradient concentration ethanol solution elution from low to high is specially：
Successively with 40% ethanol elution, 4 column volumes, 60% column volume of ethanol elution 4,80% cylinder of ethanol elution 6
Product.
The second aspect of the present invention provides the Cordyceps militaris extract of above method preparation.
The third aspect of the present invention provides above-mentioned Cordyceps militaris extract answering in the drug of preparation treatment acute kidney injury
With.
The fourth aspect of the present invention provides a kind of drug for treating acute kidney injury, by above-mentioned Cordyceps militaris extract and
Pharmaceutically acceptable auxiliary material is made.
Beneficial effects of the present invention：
The present invention is found surprisingly that the extract that Cordyceps militaris raw material is extracted by specific process can be acute separately as treating
The drug of injury of kidney can be further developed as the protection drug of acute kidney injury, have broad application prospects.Meanwhile this hair
The preparation method of bright Cordyceps militaris extract is simple, is suitble to industrialized production.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool
The technical solution of the application is described in detail in the embodiment of body.
The examination that test material is this field routine is not specifically described used in the embodiment of the present invention and comparative example
Material is tested, can be commercially available by commercial channel.
Fruiting bodies of cordyceps militaris raw material used in the embodiment of the present invention and comparative example is the same place of production, harvest of same time
Cordyceps militaris, the content of effective component is without marked difference.
Embodiment 1：
(1) it is 15% by 1kg Cordyceps militaris raw material drying to water content using fruiting bodies of cordyceps militaris as raw material, then carries out true
Empty low temperature expanding processing, the condition of the vacuum and low temperature expanding treatment are：50 DEG C of swelling temperature；Extraction vacuum number is 8 times, swollen
Changing the time is 1.5 hours.
(2) ethanol solution that the volumetric concentration of 8 times of weight is 60% is added into the raw material after vacuum and low temperature expanding treatment
Ultrasonic extraction is carried out, the condition of ultrasonic extraction is：Temperature 50 C, time 20min, power 100W, extracting solution concentration, drying obtain
To crude extract.
(3) ethanol solution that the volumetric concentration of 2 times of weight of crude extract is 50% is redissolved, passes through D101 type macroreticular resin
Column absorption, successively with 40% ethanol elution, 4 column volumes, 60% column volume of ethanol elution 4,80% cylinder of ethanol elution 6
Product collects 80% ethanol eluate, removes solvent, is concentrated into medicinal extract, and petroleum ether dissolution, filtering is then added, and filtrate removes molten
Agent is concentrated into medicinal extract, 60 DEG C of vacuum drying, crushes to get Cordyceps militaris extract is arrived.
Embodiment 2：
(1) it is 10% by 1kg Cordyceps militaris raw material drying to water content using fruiting bodies of cordyceps militaris as raw material, then carries out true
Empty low temperature expanding processing, the condition of the vacuum and low temperature expanding treatment are：45 DEG C of swelling temperature；Extraction vacuum number is 10 times,
Puffing time is 2 hours.
(2) ethanol solution that the volumetric concentration of 8 times of weight is 50% is added into the raw material after vacuum and low temperature expanding treatment
Ultrasonic extraction is carried out, the condition of ultrasonic extraction is：40 DEG C of temperature, time 30min, power 100W, extracting solution concentration, drying obtain
To crude extract.
(3) ethanol solution that the volumetric concentration of 2 times of weight of crude extract is 40% is redissolved, passes through D101 type macroreticular resin
Column absorption, successively with 40% ethanol elution, 4 column volumes, 60% column volume of ethanol elution 4,80% cylinder of ethanol elution 6
Product collects 80% ethanol eluate, removes solvent, is concentrated into medicinal extract, and petroleum ether dissolution, filtering is then added, and filtrate removes molten
Agent is concentrated into medicinal extract, 60 DEG C of vacuum drying, crushes to get Cordyceps militaris extract is arrived.
Embodiment 3：
(1) it is 20% by 1kg Cordyceps militaris raw material drying to water content using fruiting bodies of cordyceps militaris as raw material, then carries out true
Empty low temperature expanding processing, the condition of the vacuum and low temperature expanding treatment are：55 DEG C of swelling temperature；Extraction vacuum number is 6 times, swollen
Changing the time is 1 hour.
(2) ethanol solution that the volumetric concentration of 8 times of weight is 55% is added into the raw material after vacuum and low temperature expanding treatment
Ultrasonic extraction is carried out, the condition of ultrasonic extraction is：Temperature 60 C, time 20min, power 120W, extracting solution concentration, drying obtain
To crude extract.
(3) ethanol solution that the volumetric concentration of 2 times of weight of crude extract is 40% is redissolved, passes through D101 type macroreticular resin
Column absorption, successively with 40% ethanol elution, 4 column volumes, 60% column volume of ethanol elution 4,80% cylinder of ethanol elution 6
Product collects 80% ethanol eluate, removes solvent, is concentrated into medicinal extract, and petroleum ether dissolution, filtering is then added, and filtrate removes molten
Agent is concentrated into medicinal extract, 60 DEG C of vacuum drying, crushes to get Cordyceps militaris extract is arrived.
Comparative example 1：
Using fruiting bodies of cordyceps militaris as raw material, the ethanol solution that the volumetric concentration that 8 times of weight is added is 60% carries out ultrasound and mentions
It takes, the condition of ultrasonic extraction is：Temperature 50 C, time 20min, power 100W, extracting solution concentration dry, pulverize, and obtain pupa worm
Careless extract A.
Comparative example 2：
Using fruiting bodies of cordyceps militaris as raw material, the ethanol solution that the volumetric concentration of 8 times of weight is 60%, refluxing extraction 3 is added
Secondary, 3 times are respectively 2,1,0.5 hours, and extracting solution is concentrated into relative density 1.01 (50 DEG C of surveys), pass through D101 type macropore tree
Rouge column absorption, successively with 40% ethanol elution, 4 column volumes, 60% column volume of ethanol elution 4,80% column of ethanol elution 6
Volume collects 80% ethanol eluate, is concentrated into medicinal extract, 60 DEG C of vacuum drying, crushes, obtain Cordyceps militaris extract B.
Test example 1：The influence for the acute renal injury in mice that Cordyceps militaris extract induces glycerol
(1) experimental animal ICR mouse, cleaning grade, male, 22~26g of weight.
(2) modeling, grouping and administration
After mouse adaptable fed, randomized grouping is divided into blank group, model group, clearing preparation-Niaoduqing (commercially available) positive drug control group
(3.25g/kg), 1 group of test (give the Cordyceps militaris extract of the preparation of embodiment 1,100mg/kg), and 2 groups of test (gives comparative example
The Cordyceps militaris extract A of 1 preparation, 100mg/kg), 3 groups of test (gives the Cordyceps militaris extract B of the preparation of comparative example 2,100mg/
Kg), every group of 10 mouse.
After animal prohibits water non-fasting 18h, using the method for intramuscular injection, 50% glycerol is injected in leg after two, and injection is total
Amount presses 8ml/kg, and every back leg injection dosage is fifty-fifty.Wherein glycerol normal saline dilution is at 50%.Blank group injects equivalent
Physiological saline.After modeling, animal continues to prohibit water non-fasting 8h, and free diet drinks water and starts to be administered later.1 time a day,
For three days on end.The pure water of blank group, model group administration equivalent.It is administered using stomach-filling mode.
(3) acquisition, processing of blood sample and index determining
Last dose for 24 hours after, anesthetized animal, abdominal aorta blood sampling, collect its anticoagulated whole blood.Then animal is put to death, takes two
Kidney, nephridial tissue are fixed for 24 hours with 10% formaldehyde, and conventional alcohol dehydration, paraffin embedding cuts 5 μm of histotomy, haematoxylin-she
Red (HE) dyeing, the intracavitary cast quantity of om observation urinary cast.
Anticoagulated whole blood centrifugation, is centrifuged to obtain blood plasma, freezen protective by 5000r/min, 10min.It is surveyed by enzymic creatinine assay method
Determine the content of creatinine in blood plasma.
(4) experimental result
Influence of the Cordyceps militaris extract to the acute kidney injury mouse serum creatinine that glycerol induces is shown in Table 1, to glycerol induction
The influence of acute kidney injury mouse renal cast number is shown in Table 2.
Table 1：The influence for the acute kidney injury mouse serum creatinine that Cordyceps militaris extract induces glycerol
| Group | Creatinine (μm ol/L) |
| Blank group | 74.12±21.32 |
| Model group | 143.54±52.60 |
| Clearing preparation-Niaoduqing positive controls | 124.22±38.57 |
| Test 1 group | 92.26±18.35 |
| Test 2 groups | 136.40±34.82 |
| Test 3 groups | 120.25±30.20 |
Table 2：The influence for the acute kidney injury mouse renal cast number that Cordyceps militaris extract induces glycerol
| Group | Cast quantity |
| Blank group | 0 |
| Model group | 17.24±5.80 |
| Clearing preparation-Niaoduqing positive controls | 15.26±5.24 |
| Test 1 group | 7.02±2.40 |
| Test 2 groups | 16.10±4.68 |
| Test 3 groups | 14.96±5.73 |
Renal cast quantity is to evaluate one of the index that kidney structure is destroyed, and Cordyceps militaris extract is to serum creatinine
Pathological damage criterion is also set to reach statistical difference while reduction reaches statistical difference.Illustrate Cordyceps militaris extract pair
Acute kidney injury is treated with unexpected technical effect.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field
For art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repair
Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (10)
1. a kind of preparation method of Cordyceps militaris extract, which is characterized in that include the following steps：
(1) it is 10-20% by Cordyceps militaris raw material drying to water content, then carries out vacuum and low temperature expanding treatment；
(2) ethanol solution is added in the Cordyceps militaris raw material after vacuum and low temperature expanding treatment and carries out ultrasonic extraction, extracting solution concentration is done
It is dry, obtain crude extract；
(3) by crude extract with volumetric concentration be 40-60% ethanol solution redissolve, adsorbed by macroporous resin column, successively via
Low to high gradient concentration ethanol solution elution, collects 80% ethanol eluate, removes solvent, be concentrated into medicinal extract, be then added
Petroleum ether dissolution, filtering, filtrate remove solvent, and concentration, drying obtain Cordyceps militaris extract.
2. preparation method according to claim 1, which is characterized in that in step (1), the Cordyceps militaris raw material includes：Pupa
Cordyceps sinensis, fruiting bodies of cordyceps militaris or cordyceps mycelium.
3. preparation method according to claim 1, which is characterized in that in step (1), the vacuum and low temperature expanding treatment
Condition is：45-55 DEG C of swelling temperature；Extraction vacuum number is 6-10 times, and puffing time is 1-2 hours.
4. preparation method according to claim 1, which is characterized in that in step (2), the volumetric concentration of the ethanol solution
For 50-60%.
5. preparation method according to claim 1, which is characterized in that in step (2), the condition of the ultrasonic extraction is：
40-60 DEG C of temperature, time 20-30min, power 100-120W.
6. preparation method according to claim 1, which is characterized in that in step (3), the macroporous resin column is D101 type
Macroporous resin column.
7. preparation method according to claim 1, which is characterized in that in step (3), the gradient concentration from low to high
Ethanol solution elutes：
Successively with 40% ethanol elution, 4 column volumes, 60% column volume of ethanol elution 4,80% column volume of ethanol elution 6.
8. the Cordyceps militaris extract of the described in any item method preparations of claim 1-7.
9. application of the Cordyceps militaris extract according to any one of claims 8 in the drug of preparation treatment acute kidney injury.
10. a kind of drug for treating acute kidney injury, which is characterized in that by Cordyceps militaris extract according to any one of claims 8 and medicine
Acceptable auxiliary material is made on.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811073804.9A CN108815205B (en) | 2018-09-14 | 2018-09-14 | Cordyceps militaris extract and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811073804.9A CN108815205B (en) | 2018-09-14 | 2018-09-14 | Cordyceps militaris extract and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108815205A true CN108815205A (en) | 2018-11-16 |
| CN108815205B CN108815205B (en) | 2022-02-01 |
Family
ID=64149841
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811073804.9A Active CN108815205B (en) | 2018-09-14 | 2018-09-14 | Cordyceps militaris extract and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108815205B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101508715A (en) * | 2009-04-01 | 2009-08-19 | 江苏省农业科学院 | Extraction and purification process for cordycepin in cordyceps militaris link |
| CN106317148A (en) * | 2016-07-29 | 2017-01-11 | 河北省科学院生物研究所 | Method for extracting cordycepin from cordyceps militaris |
| KR20180068516A (en) * | 2016-12-14 | 2018-06-22 | 주식회사 바이오아라 | Method for extracting functional material from cordyceps militaris |
-
2018
- 2018-09-14 CN CN201811073804.9A patent/CN108815205B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101508715A (en) * | 2009-04-01 | 2009-08-19 | 江苏省农业科学院 | Extraction and purification process for cordycepin in cordyceps militaris link |
| CN106317148A (en) * | 2016-07-29 | 2017-01-11 | 河北省科学院生物研究所 | Method for extracting cordycepin from cordyceps militaris |
| KR20180068516A (en) * | 2016-12-14 | 2018-06-22 | 주식회사 바이오아라 | Method for extracting functional material from cordyceps militaris |
Non-Patent Citations (3)
| Title |
|---|
| 李学军等: ""蛹虫草中虫草素的提取及纯化工艺研究"", 《生物技术》 * |
| 胡芳等: ""蛹虫草防治高尿酸血症的实验研究"", 《中成药》 * |
| 贾淑珍等: "膨化技术在中药材加工领域的应用 ", 《农产品加工》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108815205B (en) | 2022-02-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101612243B (en) | Application of natural plant pepper extract in preparing medicine for suppressing release of histamine, cosmetic and health food | |
| CN102397461B (en) | Preparation method of tablet for women's health and tranquilness | |
| CN105169105A (en) | Chinese medicinal preparation having functions of preventing tumors, nourishing yin and stomach and enhancing immunity and preparation method thereof | |
| CN101348812A (en) | Lichenin, and preparation and use thereof | |
| CN108743600A (en) | A kind of natural drug composition and the Chinese medicine composition containing the natural drug and its application | |
| CN108403882A (en) | A kind of red sage root composition and preparation method thereof for treating coronary heart disease | |
| CN110123854B (en) | Anti-inflammatory active pharmaceutical composition based on bupleurum chinense component and application thereof | |
| CN101549010B (en) | A preparing method and application of malaytea scurfpea fruit total glycosides extract | |
| CN108815205A (en) | Cordyceps militaris extract and the preparation method and application thereof | |
| CN103989740B (en) | Application of the rabdosia japonica in resisting acute lung injury medicine is prepared | |
| CN103655544B (en) | The application of Jaceosidin in preparation prevention or the Fibrotic medicine for the treatment of chronic hepatic injury regulating liver-QI | |
| CN102462710A (en) | Application of sunset abelmoschus flower total flavone to preparation of medicament for preventing and treating hepatofibrosis | |
| CN102228666B (en) | Composition prepared from pine pollen and curcuma, preparation method thereof, and application of composition in preparing medicament for treating inflammatory bowel disease | |
| AU2021101775A4 (en) | Cordyceps militaris Extract and Its Preparation and Application | |
| CN108159114B (en) | Lamiophlomis rotata total iridoid glycoside extract, extraction method and application thereof | |
| CN105343109A (en) | Application of cycloastragenol in preparation of drugs for protecting liver and promoting liver injury restoration | |
| CN101804128B (en) | Medicine composition, and use thereof in preparing medicine for curing inflammatory enteropathy | |
| CN115844933B (en) | Application of clindamycin total flavone in preparing heart failure resisting medicine | |
| CN109331153A (en) | A kind of Medical-use cold compress gel and preparation method thereof for treating children's dermatitis and eczema | |
| CN103393928B (en) | High-capacity pulse-activating injection and preparation method thereof | |
| CN1947737B (en) | Application of psammophytes strawflower total saponin in preparing medicine | |
| AU2021105573A4 (en) | Application of emodin in inhibiting the proliferation of alcohol-induced activated hepatic stellate cells in the treatment of liver fibrosis | |
| CN107485655A (en) | Flos persicae extract is preparing the application in treating prostatitis medicine | |
| CN109939176B (en) | A Chinese medicinal composition for treating diabetes, and its preparation method | |
| CN106913599A (en) | Maidenhair extractive of general flavone preparation method and applied in resistance prostatitis medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230721 Address after: 1220-8, Floor 12, Office Building 1, No. 16, Xincheng 17th Road, Changqing Street, Dongxihu District, Wuhan City, 430000, Hubei Province Patentee after: Wuhan Topaz Intellectual Property Operation Co.,Ltd. Address before: 221018 Lishui Road, Yunlong District, Xuzhou, Jiangsu 2 Patentee before: XUZHOU University OF TECHNOLOGY Effective date of registration: 20230721 Address after: 443500 Innovation Industrial Park, Changyang Tujia Autonomous County, Yichang City, Hubei Province Patentee after: HUBEI RENREN BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. Address before: 1220-8, Floor 12, Office Building 1, No. 16, Xincheng 17th Road, Changqing Street, Dongxihu District, Wuhan City, 430000, Hubei Province Patentee before: Wuhan Topaz Intellectual Property Operation Co.,Ltd. |