CN108795805A - Brevibacillus laterosporus fermentation modification method - Google Patents
Brevibacillus laterosporus fermentation modification method Download PDFInfo
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- CN108795805A CN108795805A CN201810584034.8A CN201810584034A CN108795805A CN 108795805 A CN108795805 A CN 108795805A CN 201810584034 A CN201810584034 A CN 201810584034A CN 108795805 A CN108795805 A CN 108795805A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of Brevibacillus laterosporus fermentation modification method, seeding tank and fermentation tank fill fermentation medium, basal medium are used before accessing seeding tank；Basal medium was in shaking table culture 18-20 hours, and seeding tank is cultivated 18-20 hours after connecing bacterium, culture tank culture 20 hours；The advantage of the invention is that：Bean cake powder is changed to by beancake powder to main nitrogen, viable count significantly improves 10-100 times, reaches 1013/ ml, improves work efficiency, and increases economic effect.
Description
Technical field
The present invention relates to a kind of fermentations to change method, and specifically a kind of Brevibacillus laterosporus fermentation modification method belongs to
Fermentation changes method field.
Background technology
Brevibacillus laterosporus is a kind of agricultural microorganism, and zymotechnique viable count traditional at present has reached 1011/
ml.The raw material nitrogen source used of fermenting is mainly beancake powder.Obtained viable count still has certain room for promotion.
Invention content
To solve the above-mentioned problems, the present invention devises a kind of Brevibacillus laterosporus fermentation modification method, to main nitrogen
Source is changed to bean cake powder by beancake powder, and viable count significantly improves 10-100 times, reaches 1013/ ml, improves work efficiency, and increases
Economic effect.
The technical scheme is that：
Brevibacillus laterosporus fermentation modification method, concrete operations are：Seeding tank and fermentation tank fill fermentation medium, access kind
Basal medium is used before sub- tank；Basal medium in shaking table culture 18-20 hours, after connecing bacterium cultivate 18-20 hours by seeding tank, training
Support tank culture 20 hours.
Above-mentioned cultivation temperature is 35-39 DEG C, and the ventilatory capacity of seeding tank and fermentation tank is 1:0.8-1.0, tank pressure are 0.06-
0.08 megapascal.
The advantage of the invention is that：Bean cake powder is changed to by beancake powder to main nitrogen, viable count significantly improves 10-100 times,
Reach 1013/ ml, improves work efficiency, and increases economic effect.
The present invention is further explained in the light of specific embodiments.
Specific implementation mode
Hereinafter, preferred embodiments of the present invention will be described, it should be understood that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
The percentage sign " % " arrived involved in the present invention refers to mass percent if not specified；But the percentage of solution,
Unless otherwise specified, refer to containing several grams of solute in solution 100ml；Percentage between liquid, capacity when referring to 20 DEG C
Ratio.
Embodiment 1
A kind of Brevibacillus laterosporus fermentation modification method, concrete operations are：Seeding tank and fermentation tank fill fermentation medium, connect
Basal medium is used before entering seeding tank；Basal medium was in shaking table culture 18-20 hours, and culture 18-20 is small after seeding tank connects bacterium
When, culture tank culture 20 hours.
Above-mentioned cultivation temperature is 35-39 DEG C, and the ventilatory capacity of seeding tank and fermentation tank is 1:0.8-1.0, tank pressure are 0.06-
0.08 megapascal.
Practical application example：Basal medium shakes 18 hours, 120 turns per minute, and temperature is 37 DEG C, seeding tank 18 hours,
Fermentation tank is 20 hours, ventilatory capacity 1:0.8, tank presses 0.08 megapascal, fermentation process：Strain accesses basal medium, is trained with shaking table
It supports, cultivation temperature is that 37 DEG C of incubation times are 18 hours, and 100 liters of seeding tanks are transferred to after culture, and tank presses 0.08 megapascal, ventilation
Amount is 1：0.8（V/V）, cultivate 18 hours, go to 1 ton of fermentation tank and continue to ferment, tank is put after 20 hours.
It is 3 × 10 to put tank detection viable count13PH is 7 or so.
Corn flour and bean cake powder corn flour are superfine powder.
Raw materials used in the present invention is to commonly use raw material in the production of this field, can be obtained from market, and for production
As a result it will not have an impact；Various equipment employed in the present invention are the conventional equipment used in the production technology of this field,
And operation, the parameter etc. of each equipment are carried out according to routine operation, have no special feature.
Claims (2)
- The modification method 1. Brevibacillus laterosporus ferments, which is characterized in that method is as follows：Seeding tank and fermentation tank fill fermentation medium, and basal medium is used before accessing seeding tank；Basal medium is trained in shaking table It supports 18-20 hours, seeding tank is cultivated 18-20 hours after connecing bacterium, culture tank culture 20 hours.
- The modification method 2. Brevibacillus laterosporus according to claim 1 ferments, which is characterized in that concrete operations are：Basal medium shakes 18 hours, 120 turns per minute, and temperature is 37 degree, seeding tank 18 hours, and fermentation tank is 20 hours, is led to Tolerance 1:0.8, tank presses 0.08 megapascal, fermentation process：Strain accesses basal medium, and with shaking table culture, cultivation temperature is 37 degree Incubation time is 18 hours, and 100 liters of seeding tanks are transferred to after culture, and tank presses 0.08 megapascal, ventilatory capacity 1：0.8, culture 18 Hour, it goes to 1 ton of fermentation tank and continues to ferment, tank is put after 20 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810584034.8A CN108795805A (en) | 2018-06-08 | 2018-06-08 | Brevibacillus laterosporus fermentation modification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810584034.8A CN108795805A (en) | 2018-06-08 | 2018-06-08 | Brevibacillus laterosporus fermentation modification method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108795805A true CN108795805A (en) | 2018-11-13 |
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ID=64087632
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810584034.8A Pending CN108795805A (en) | 2018-06-08 | 2018-06-08 | Brevibacillus laterosporus fermentation modification method |
Country Status (1)
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101863685A (en) * | 2010-06-07 | 2010-10-20 | 王来福 | Method for making high-concentration bacterial powder for microbial fertilizers |
| CN102524518A (en) * | 2012-01-21 | 2012-07-04 | 河北科技大学 | Method for producing antibacterial peptide by using brevibacillus laterosporu |
| CN103289937A (en) * | 2013-06-18 | 2013-09-11 | 东莞市保得生物工程有限公司 | Method for producing bacillus laterosporus live bacteria by high density solid fermentation |
| CN104004803A (en) * | 2014-05-22 | 2014-08-27 | 河北科技大学 | Method for producing antimicrobial peptide through fermentation of brevibacillus laterosporu |
| CN105594698A (en) * | 2015-12-19 | 2016-05-25 | 佛山市艳晖生物科技有限公司 | Bacillus laterosporus wettable powder preparation method |
| CN107384841A (en) * | 2017-09-15 | 2017-11-24 | 延边大学 | A kind of microbial inoculum prevented and treated lepidoptera pest and preparation method thereof |
-
2018
- 2018-06-08 CN CN201810584034.8A patent/CN108795805A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101863685A (en) * | 2010-06-07 | 2010-10-20 | 王来福 | Method for making high-concentration bacterial powder for microbial fertilizers |
| CN102524518A (en) * | 2012-01-21 | 2012-07-04 | 河北科技大学 | Method for producing antibacterial peptide by using brevibacillus laterosporu |
| CN103289937A (en) * | 2013-06-18 | 2013-09-11 | 东莞市保得生物工程有限公司 | Method for producing bacillus laterosporus live bacteria by high density solid fermentation |
| CN104004803A (en) * | 2014-05-22 | 2014-08-27 | 河北科技大学 | Method for producing antimicrobial peptide through fermentation of brevibacillus laterosporu |
| CN105594698A (en) * | 2015-12-19 | 2016-05-25 | 佛山市艳晖生物科技有限公司 | Bacillus laterosporus wettable powder preparation method |
| CN107384841A (en) * | 2017-09-15 | 2017-11-24 | 延边大学 | A kind of microbial inoculum prevented and treated lepidoptera pest and preparation method thereof |
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Application publication date: 20181113 |
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| RJ01 | Rejection of invention patent application after publication |