CN108794562A - A kind of processing technology of sapindoside extract - Google Patents
A kind of processing technology of sapindoside extract Download PDFInfo
- Publication number
- CN108794562A CN108794562A CN201811001259.2A CN201811001259A CN108794562A CN 108794562 A CN108794562 A CN 108794562A CN 201811001259 A CN201811001259 A CN 201811001259A CN 108794562 A CN108794562 A CN 108794562A
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- Prior art keywords
- soapberry
- processing technology
- sapindoside
- drying
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
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- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
- Steroid Compounds (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a kind of processing technologys of sapindoside extract, it is dried including soapberry, soapberry acidolysis grinding, thickening filtration, concentration and recovery, saponin extraction, ethyl alcohol extracts, it can make active ingredient Fast Stripping, have many advantages, such as that recovery rate is high, solvent for use is safe and non-toxic, consumption is few；Simultaneously to having carried out sulfuric acid sour water solution before being extracted using petroleum ether and ethyl acetate, simultaneously after sulfuric acid sour water solution, subsequent dehydration is also allowed for, compare first alcohol and water, this kind of mode is separated off a large amount of impurity without later-period purification, and technique extraction time of the present invention is few, and recovery rate greatly improves, extractant is recycled simultaneously, it is cost-effective.
Description
Technical field：
The present invention relates to pharmaceutical processing techniques field, more particularly to a kind of processing technology of sapindoside extract.
Background technology：
Soapberry, deciduous tree, branch are carried out, leaf alternate；Without stipule；There is handle；Panicle, basidixed and side life；Flower polygamy,
Corolla light green, there is shortly clawed；Floral disc cup-shaped；Filigree has fine, soft fur, medicine back life, and hermaphrodite flower stamen is small, and filigree has fur.Drupe
Spherical shape, yellow or brown color when ripe.Seed is spherical, black, 6~July of florescence, 9~October of fruiting period, the Yangtze river basin originating in China with
Southern various regions and South East Asia Mainland various regions, India and Japan.Pericarp contains saponin, can do boxboard and wooden comb etc. for soap, timber.
At present for sapindoside extraction, it is substantially all using methanol extraction or by being extracted with water, this two classes method carries
Take effect low, impurity is more, subsequently also purifies and separates is needed to detach, and is unfavorable for mass producing.
Invention content：
The purpose of the present invention is that solve the above-mentioned problems and provides a kind of processing work of sapindoside extract
Skill, when solving sapindoside extraction of the existing technology, extraction effect is low, the problem more than impurity.
To solve the above-mentioned problems, the present invention provides a kind of technical solutions：
A kind of processing technology of sapindoside extract, including soapberry drying, soapberry acidolysis grind, are concentrated
Filter, concentration and recovery, saponin extraction.
Preferably, the step of soapberry drying, is as follows：
A, it dries：By soapberry natural drying 1-3 days of harvest, then low-temperature aeration is stored, and avoids sunlight；
B, the soapberry after the completion of drying is put into drying box, the temperature in drying box is maintained at 60 DEG C or so, is held
Continuous 30min；
Preferably, the step of soapberry hydrolysis grinding, is as follows：
A, the soapberry after drying is subjected to acidolysis with 1mol/L sulfuric acid；
B, soapberry uses 5%Na2CO3 aqueous hydrolysis at neutrality again after acidolysis；
C, the soapberry after hydrolysis carry out again drying be then ground to it is powdered.
Preferably, the step of thickening filtration, is as follows：
A, the soapberry after grinding is put into extractor, the extraction of 60-80% high purity ethanols is added twice, repeats several
It is secondary, then in recycling ethyl alcohol, until being concentrated to solvent-free taste.
Preferably, steps are as follows for the concentration and recovery：
A, reagent petroleum ether is added into concentrate, at normal temperatures, petroleum ether cycling extraction is added twice, stratification,
Supernatant is put into concentration tank, and recycling design petroleum ether is concentrated under reduced pressure；
B, lower subnatant continues to be put into extractor, and solvent ethyl acetate is recovered under reduced pressure.
Preferably, the step of saponin extraction, is as follows：
A, oil-containing sapindoside sediment is staticly settled 15-20 minutes, the clear liquid for precipitating top layer is removed；
B, sediment is carried out dehydrating by centrifuge；
C, ethyl acetate is added, is extracted twice at normal temperatures, is for the first time 2 times of concentrate, second is concentration
1.5 times of liquid, take supernatant；
D, solvent ethyl acetate is recovered under reduced pressure, saponin(e liquid cooling is frozen；
E, it is filtered after freezing, is then dried in vacuo in vacuum filter, finally obtain to obtain saponin(e.
Preferably, needing the heavy of the modified cellulose that 2000-4500mPas is added during the centrifugation
Shallow lake agent, and the mixture that the precipitating reagent is carboxymethyl cellulose, hydroxyethyl cellulose and hydroxypropyl methyl cellulose.
Beneficial effects of the present invention：Ethyl alcohol extracts, and can make active ingredient Fast Stripping, has recovery rate high, used molten
The advantages that agent is safe and non-toxic, consumption is few；Simultaneously to having carried out sulfuric acid acid before being extracted using petroleum ether and ethyl acetate
Hydrolysis, while after sulfuric acid sour water solution, subsequent dehydration has been also allowed for, first alcohol and water is compared, this kind of mode is pure without the later stage
Change is separated off a large amount of impurity, and technique extraction time of the present invention is few, and recovery rate greatly improves, while being carried out to extractant
It recycles, it is cost-effective.
Specific implementation mode：
Present embodiment uses following technical scheme：A kind of processing technology of sapindoside extract, including nothing
Suffer from son drying, soapberry acidolysis grinding, thickening filtration, concentration and recovery, saponin extraction.
Wherein, the step of soapberry drying is as follows：
A, it dries：By soapberry natural drying 1-3 days of harvest, then low-temperature aeration is stored, and avoids sunlight；
B, the soapberry after the completion of drying is put into drying box, the temperature in drying box is maintained at 60 DEG C or so, is held
Continuous 30min.
Wherein, the step of soapberry hydrolysis grinding is as follows：
A, the soapberry after drying is subjected to acidolysis with 1mol/L sulfuric acid；
B, soapberry uses 5%Na2CO3 aqueous hydrolysis at neutrality again after acidolysis；
C, the soapberry after hydrolysis carry out again drying be then ground to it is powdered；
Wherein, the step of thickening filtration is as follows：
A, the soapberry after grinding is put into extractor, the extraction of 60-80% high purity ethanols is added twice, repeats several
It is secondary, then in recycling ethyl alcohol, until being concentrated to solvent-free taste.
Wherein, steps are as follows for the soapberry concentration and recovery：
A, reagent petroleum ether is added into concentrate, at normal temperatures, petroleum ether cycling extraction is added twice, stratification,
Supernatant is put into concentration tank, and recycling design petroleum ether is concentrated under reduced pressure；
B, lower subnatant continues to be put into extractor, and solvent ethyl acetate is recovered under reduced pressure.
Wherein, the step of saponin extraction is as follows：
A, oil-containing sapindoside sediment is staticly settled 15-20 minutes, the clear liquid for precipitating top layer is removed；
B, sediment is carried out dehydrating by centrifuge；
C, ethyl acetate is added, is extracted twice at normal temperatures, is for the first time 2 times of concentrate, second is concentration
1.5 times of liquid, take supernatant；
D, solvent ethyl acetate is recovered under reduced pressure, saponin(e liquid cooling is frozen；
E, it is filtered after freezing, is then dried in vacuo in vacuum filter, finally obtain to obtain saponin(e.
Wherein, the precipitating reagent that the modified cellulose of 2000-4500mPas is added is needed during the centrifugation,
And the mixture that the precipitating reagent is carboxymethyl cellulose, hydroxyethyl cellulose and hydroxypropyl methyl cellulose.
Beneficial effects of the present invention：Ethyl alcohol extracts, and can make active ingredient Fast Stripping, has recovery rate high, used molten
The advantages that agent is safe and non-toxic, consumption is few；Simultaneously to having carried out sulfuric acid acid before being extracted using petroleum ether and ethyl acetate
Hydrolysis, while after sulfuric acid sour water solution, subsequent dehydration has been also allowed for, first alcohol and water is compared, this kind of mode is pure without the later stage
Change is separated off a large amount of impurity, and technique extraction time of the present invention is few, and recovery rate greatly improves, while being carried out to extractant
It recycles, it is cost-effective.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention, the technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention, the claimed scope of the invention.
Claims (7)
1. a kind of processing technology of sapindoside extract, which is characterized in that dried including soapberry, soapberry acidolysis is ground
Mill, thickening filtration, concentration and recovery, saponin extraction.
2. a kind of processing technology of sapindoside extract according to claim 1, it is characterised in that：The soapberry
The step of drying, is as follows：
A, it dries：By soapberry natural drying 1-3 days of harvest, then low-temperature aeration is stored, and avoids sunlight；
B, the soapberry after the completion of drying is put into drying box, the temperature in drying box is maintained at 60 DEG C or so, continued
30min。
3. a kind of processing technology of sapindoside extract according to claim 1, it is characterised in that：The soapberry
The step of hydrolysis grinding, is as follows：
A, the soapberry after drying is subjected to acidolysis with 1mol/L sulfuric acid；
B, soapberry uses 5%Na2CO3 aqueous hydrolysis at neutrality again after acidolysis；
C, the soapberry after hydrolysis carry out again drying be then ground to it is powdered.
4. a kind of processing technology of sapindoside extract according to claim 1, it is characterised in that：It is described concentrated
The step of filter, is as follows：
A, the soapberry after grinding is put into extractor, the extraction of 60-80% high purity ethanols is added twice, repeated several times,
Then in recycling ethyl alcohol, until being concentrated to solvent-free taste.
5. a kind of processing technology of sapindoside extract according to claim 1, it is characterised in that：The soapberry
Steps are as follows for concentration and recovery：
A, reagent petroleum ether is added into concentrate, at normal temperatures, petroleum ether cycling extraction is added twice, stratification, supernatant
Liquid is put into concentration tank, and recycling design petroleum ether is concentrated under reduced pressure；
B, lower subnatant continues to be put into extractor, and solvent ethyl acetate is recovered under reduced pressure.
6. a kind of processing technology of sapindoside extract according to claim 1, it is characterised in that：The saponin(e carries
The step of taking is as follows：
A, oil-containing sapindoside sediment is staticly settled 15-20 minutes, the clear liquid for precipitating top layer is removed；
B, sediment is carried out dehydrating by centrifuge；
C, ethyl acetate is added, is extracted twice at normal temperatures, is for the first time 2 times of concentrate, second is concentrate
1.5 times, take supernatant；
D, solvent ethyl acetate is recovered under reduced pressure, saponin(e liquid cooling is frozen；
E, it is filtered after freezing, is then dried in vacuo in vacuum filter, finally obtain to obtain saponin(e.
7. a kind of processing technology of sapindoside extract according to claim 1, it is characterised in that：The centrifugation is heavy
The precipitating reagent that the modified cellulose of 2000-4500mPas is added is needed during shallow lake, and the precipitating reagent is carboxymethyl cellulose
The mixture of element, hydroxyethyl cellulose and hydroxypropyl methyl cellulose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811001259.2A CN108794562A (en) | 2018-08-30 | 2018-08-30 | A kind of processing technology of sapindoside extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811001259.2A CN108794562A (en) | 2018-08-30 | 2018-08-30 | A kind of processing technology of sapindoside extract |
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| Publication Number | Publication Date |
|---|---|
| CN108794562A true CN108794562A (en) | 2018-11-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811001259.2A Pending CN108794562A (en) | 2018-08-30 | 2018-08-30 | A kind of processing technology of sapindoside extract |
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| CN (1) | CN108794562A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702843A (en) * | 2019-09-30 | 2020-01-17 | 佛山市欧若拉生物科技有限公司 | Non-calibration amount determination kit and determination method for soapberry saponin standard substance |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302600A (en) * | 2000-12-25 | 2001-07-11 | 昆明国花生物制品有限公司 | Series products containing Chinese soapherry extract |
| CN102241725A (en) * | 2010-05-13 | 2011-11-16 | 福建源华林业生物科技有限公司 | Water extraction preparation method of daily chemical sapindoside |
| CN102494928A (en) * | 2011-11-18 | 2012-06-13 | 福建省源容生物科技有限公司 | Preparation method for sapindoside production quality control grading standard product |
| CN103992358A (en) * | 2014-04-25 | 2014-08-20 | 湖南春源生物科技开发集团有限公司 | Extraction method for sapindus-saponin |
| CN106083983A (en) * | 2016-06-20 | 2016-11-09 | 福州大学 | A kind of method preparing helexin from Fructus Sapindi Mukouossi |
-
2018
- 2018-08-30 CN CN201811001259.2A patent/CN108794562A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302600A (en) * | 2000-12-25 | 2001-07-11 | 昆明国花生物制品有限公司 | Series products containing Chinese soapherry extract |
| CN102241725A (en) * | 2010-05-13 | 2011-11-16 | 福建源华林业生物科技有限公司 | Water extraction preparation method of daily chemical sapindoside |
| CN102494928A (en) * | 2011-11-18 | 2012-06-13 | 福建省源容生物科技有限公司 | Preparation method for sapindoside production quality control grading standard product |
| CN103992358A (en) * | 2014-04-25 | 2014-08-20 | 湖南春源生物科技开发集团有限公司 | Extraction method for sapindus-saponin |
| CN106083983A (en) * | 2016-06-20 | 2016-11-09 | 福州大学 | A kind of method preparing helexin from Fructus Sapindi Mukouossi |
Non-Patent Citations (1)
| Title |
|---|
| 饶厚曾 等: "无患子皂苷提取工艺研究", 《江西科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702843A (en) * | 2019-09-30 | 2020-01-17 | 佛山市欧若拉生物科技有限公司 | Non-calibration amount determination kit and determination method for soapberry saponin standard substance |
| CN110702843B (en) * | 2019-09-30 | 2022-05-17 | 东莞微领生物科技有限公司 | Non-calibration amount determination kit and determination method for soapberry saponin standard substance |
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Application publication date: 20181113 |
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